LOCUS       NZ_AP023354            10414 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Actinocatenispora sera strain NBRC 101916 chromosome.
ACCESSION   NZ_AP023354
VERSION     NZ_AP023354.1
KEYWORDS    RefSeq.
SOURCE      Actinocatenispora sera
  ORGANISM  Actinocatenispora sera
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinocatenispora.
REFERENCE   1
  AUTHORS   Komaki,H. and Tamura,T.
  TITLE     Whole genome shotgun sequence of Actinocatenispora sera NBRC 101916
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7044238)
  AUTHORS   Ichikawa,N., Komaki,H., Tamura,T. and Uohara,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2020) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :https://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4
            Genome Coverage                   :: 251x
            Sequencing Technology             :: SMRT cell 8 Pac V3
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018324685.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 04:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,585
            CDSs (total)                      :: 6,501
            Genes (coding)                    :: 6,404
            CDSs (with protein)               :: 6,404
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 26 of 97
            Pseudo Genes (incomplete)         :: 81 of 97
            Pseudo Genes (internal stop)      :: 1 of 97
            Pseudo Genes (multiple problems)  :: 10 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:48:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5846054
            Orig. end                         :: 5856468
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Annotated at DFAST https://dfast.nig.ac.jp/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="16"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(768..783)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.72"
                     /tool="antismash"
     gene            1339..3891
                     /gene="otsB"
                     /locus_tag="Asera_RS27470"
                     /old_locus_tag="Asera_55200"
     CDS             1339..3891
                     /EC_number="3.1.3.12"
                     /GO_function="GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="otsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786934.1"
                     /locus_tag="Asera_RS27470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55200"
                     /product="trehalose-phosphatase"
                     /protein_id="WP_030444294.1"
                     /transl_table=11
                     /translation="MSTVAPTEPLDAALRETLGRLARVPQLLVAVDYDGTLAPIVGDPA
                     AARPLPEAVTALRALAELPQTKVAVVSGRSLRDLAALSRLPSEIHLVGSHGSEFDVGFV
                     QQLSEEARQLRTTLQQQVQRIAAGAPGVRLEAKPASIAVHTRTASDEVAASVNAAVRSG
                     PATLPGVHVTEGKKVIELSVVHTDKGTALDTLRSRLGASAVLFVGDDVTDENGFARLRG
                     PDVGVKVGFGDTRATHRVADPIEVAKLLALVLDIRRRWLYGERAVPIERHSMLAGGDNL
                     ALVTPDARITWLCHPRPDSAAIFADLLGGSPAGYFGVAPQRGGLPLGQKYLGGSMTVQT
                     RWSGLTVTDWLDSTGPANRTTLVRQLTGTVTVRVQFAPRPEFGQVAVRLQPLGDGLVML
                     GSNEPAVLYSPGVDWDIVQEGEHDTAHALIDLRAAGGSAVLELRCGTDSLDPAPLPVEH
                     RADETATQWRRWLDGLRLPGVSTELVARSALTLRALTYRPTGAILAAGTSSLPEDLGGV
                     RNWDYRYCWLRDAALAARALVDLGSVDEAEGLLAWTAGVIDRTGGHPERLHPLYTVEGQ
                     TLGPEAVIDTLPGYAGSRPVRVGNAANKQLQLDVFGPIADLVDAVAQRRGVISDFDREV
                     LSAMVDAVAHRWFEPDHGIWEQRQPPRHHVYSKVMCWQTVDRALNVAARHGLPERPDWV
                     ELRAAIAENVLEYGWNEQVGAYTTAYDGIDLDAASLWVGLSGLLADDDPRFLSTVLAIE
                     AELRSGPVVYRYRSDDGMPGTEGGFHVCTAWLIESYLRTGRRADAEELYGQLIACAGPT
                     GLLPEQYDPLAERGLGNHPQAYSHLGLIRCALLLDSRP"
     misc_feature    2698..2700
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4096..5004)
                     /gene="thpD"
                     /locus_tag="Asera_RS27475"
                     /old_locus_tag="Asera_55210"
     CDS             complement(4096..5004)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR02408.1"
                     /locus_tag="Asera_RS27475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55210"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_030444293.1"
                     /sec_met_domain="PhyH (E-value: 6.5e-36, bitscore: 123.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTVTLPTTVPDEYPTRKRPTPSLIFRRDPVVWSTGATGPIDTQ
                     TLEDYDKNGYLSVDQLLTDAEVELYSAELDRLATDPAVRADERTVVEKSSNEVRSIFEV
                     HKISELVAKLVADERVVGRARQILGSDVYVHQSRINYKPGFGGAGFYWHSDFETWHAED
                     GMPAMRACSISIALTENFAQNGCLMIMPGSHRTFVSCVGETPAEHYKQSLKEQEIGTPD
                     QDSLKLLANKHGIEQFTGPAGSATIFDCNCMHGSNGNITPWPRSNIFVVFNSVENATVE
                     PFAAPSPRPPFIASRDFTPVG"
     gene            complement(5001..5414)
                     /locus_tag="Asera_RS27480"
                     /old_locus_tag="Asera_55220"
     CDS             complement(5001..5414)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="Asera_RS27480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55220"
                     /product="ectoine synthase"
                     /protein_id="WP_030444292.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.5e-53, bitscore:
                     176.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLADITETDRDVKAATWRSKRLVLAKEKVGFSLHETVMYAG
                     TETHMWYANHIEAVLCIEGEAELTNAETGEKHHIVPGTMYLLDGHEHHIMRPITDIRTI
                     CVFNPPVTGREVHDDNGVYPLIVEEETTQEVSA"
     gene            complement(5427..6677)
                     /gene="ectB"
                     /locus_tag="Asera_RS27485"
                     /old_locus_tag="Asera_55230"
     CDS             complement(5427..6677)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 1.2e-76.
                     Score: 249.9. Matches aSDomain:
                     nrpspksdomains_Asera_RS27485_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084079.1"
                     /locus_tag="Asera_RS27485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55230"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_030444291.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.4e-77, bitscore:
                     257.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLFADLESEVRSYCRTWPVAFDHAVGSRMYDENGRPYLDFFAGA
                     GALNYGHNHPELKRALLAYLERDGVTHGLDMHTTAKRDLLAALREYVLAPRGLDYKVQF
                     PGPTGTNAVEAALKLARKVTGRESIISFTHGFHGMTLGSLSVTGNAMKRGGAGIPLVHA
                     TPMPFDNYLDGQVPDFLWFERLLADSGSGLNAPAAVIVETVQGEGGINVARPEWLRSLA
                     ELCKRHDILLIVDDVQMGCGRTGPFFSFEVAGIEPDIVCLSKSIGGYGLPLALVLLRPE
                     LDVWSPGEHNGTFRGNNPAFVTAARALELFWTDDLLPAQTATKGEVVESALTAIASKHA
                     ELDIAVRGRGLAWGLAFADADVAAKVVTGAFDRGLLIETAGADGEVAKLMPALTIGHDE
                     LGEGLEILTDAVADAAH"
     aSDomain        complement(5607..6611)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Asera_RS27485_Aminotran_3.1"
                     /evalue="1.20E-76"
                     /label="Asera_RS27485_Aminotran_3.1"
                     /locus_tag="Asera_RS27485"
                     /protein_end="357"
                     /protein_start="22"
                     /score="249.9"
                     /tool="antismash"
                     /translation="DHAVGSRMYDENGRPYLDFFAGAGALNYGHNHPELKRALLAYLER
                     DGVTHGLDMHTTAKRDLLAALREYVLAPRGLDYKVQFPGPTGTNAVEAALKLARKVTGR
                     ESIISFTHGFHGMTLGSLSVTGNAMKRGGAGIPLVHATPMPFDNYLDGQVPDFLWFERL
                     LADSGSGLNAPAAVIVETVQGEGGINVARPEWLRSLAELCKRHDILLIVDDVQMGCGRT
                     GPFFSFEVAGIEPDIVCLSKSIGGYGLPLALVLLRPELDVWSPGEHNGTFRGNNPAFVT
                     AARALELFWTDDLLPAQTATKGEVVESALTAIASKHAELDIAVRGRGLAWGLAF"
     misc_feature    6666..6681
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.96"
                     /tool="antismash"
     gene            complement(6705..7256)
                     /gene="ectA"
                     /locus_tag="Asera_RS27490"
                     /old_locus_tag="Asera_55240"
     CDS             complement(6705..7256)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /locus_tag="Asera_RS27490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55240"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_211255447.1"
                     /transl_table=11
                     /translation="MTGQPQDSPSATVESMTDDRGGSVVFRAPTVADGTRLWRLAADSG
                     TLDVNSEYSYLLWCRDFAATSVVGCVGDSVASFVTGYLRPDAPDTLFVWQVAVDAAYRR
                     RGLAVRALDALVDRVARTQSVVFLEATVTPDNVPSARLFAGFARARGASLTKQSLFTEE
                     HFSGGPHNEEVLFRIGPLPR"
     misc_feature    complement(7350..7366)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.49"
                     /tool="antismash"
     misc_feature    7356..7369
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.26"
                     /tool="antismash"
     misc_feature    7357..7370
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.76"
                     /tool="antismash"
     misc_feature    complement(7359..7373)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.19"
                     /tool="antismash"
     gene            complement(7520..8773)
                     /gene="cobA"
                     /locus_tag="Asera_RS27495"
                     /old_locus_tag="Asera_55250"
     CDS             complement(7520..8773)
                     /EC_number="2.1.1.107"
                     /GO_function="GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cobA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351347.1"
                     /locus_tag="Asera_RS27495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55250"
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="WP_030444289.1"
                     /sec_met_domain="TP_methylase (E-value: 2.7e-49, bitscore:
                     166.6, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSELYPLGLRLAGRPVLVVGGGAVAQRRIPALLAADAVVTLVAPA
                     ATPALDERASLGEIHWQQRAFTPSDVDGQWLVLAATDDPAANAAVSDAASQRRVFCVRA
                     DDAAAASAWTPTVTGADELTVAVWGGGDPRRSVRLREAIAAGLADGSLDSPRFRHPEPS
                     PAGRLVPASPRPVPGVALVGAGPGDPELITVRGRRLLSRANVVVVDRLAPQLLLDGLRG
                     DVEVIDAAKIPRGRQLAQESINEVLVERALAGRFVVRLKGGDPFVFGRGGEEAAACLAA
                     GVPVTVVPGVSSAIAVPGLAGIPVTHRGLTHELVVCSGHLPPEHPGSLVDWAALARLRG
                     TVVLLMAVANLASIAARLIELGRAGGTPAAVVERGGTPQQRTVRATLATIAAAADRQGI
                     GAPAVVVIGPVVDAIGAG"
     gene            complement(8770..9843)
                     /gene="cobC"
                     /locus_tag="Asera_RS27500"
                     /old_locus_tag="Asera_55260"
     CDS             complement(8770..9843)
                     /EC_number="4.1.1.81"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (55-346). E-value:
                     1.4e-31. Score: 101.8. Matches aSDomain:
                     nrpspksdomains_Asera_RS27500_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="cobC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014753.1"
                     /locus_tag="Asera_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_55260"
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="WP_030444288.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.6e-29, bitscore:
                     101.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASRERLVALPERSLAADAVDLSHHGDTELAPGLVDLAVNVRHEP
                     APGWLADAVRAAVDDLAHYPDPGPARGAVAARHGRPESEVLLTAGAAEAFVLLARALPV
                     HRAVLVHPQFTEPEAALRSAGHRVDRVLLAPPFELAPELVPDDADLVMIGNPTNPTSVL
                     HPAETIAGLCRPGRIVVVDEAFADTVPGEPESLAGRRDLPGLVVIRSFTKTWGLAGLRV
                     GYLLGAADVVARCAAAQPLWPVSSPALAAARVLAEPRAVAAERRIAEQLDTDRRYLMAR
                     LAAAELPVVGVPRSRFVLVHRPGADRLREALRSAGYAVRRGDTFPGLGPDWLRITVRDA
                     GTTDGFVAALAAAGGAR"
     aSDomain        complement(8806..9678)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Asera_RS27500_Aminotran_1_2.1"
                     /evalue="1.40E-31"
                     /label="Asera_RS27500_Aminotran_1_2.1"
                     /locus_tag="Asera_RS27500"
                     /protein_end="346"
                     /protein_start="55"
                     /score="101.8"
                     /tool="antismash"
                     /translation="AAVDDLAHYPDPGPARGAVAARHGRPESEVLLTAGAAEAFVLLAR
                     ALPVHRAVLVHPQFTEPEAALRSAGHRVDRVLLAPPFELAPELVPDDADLVMIGNPTNP
                     TSVLHPAETIAGLCRPGRIVVVDEAFADTVPGEPESLAGRRDLPGLVVIRSFTKTWGLA
                     GLRVGYLLGAADVVARCAAAQPLWPVSSPALAAARVLAEPRAVAAERRIAEQLDTDRRY
                     LMARLAAAELPVVGVPRSRFVLVHRPGADRLREALRSAGYAVRRGDTFPGLGPDWLRIT
                     VRDAGTTDGF"
     misc_feature    9839..9852
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.36"
                     /tool="antismash"
ORIGIN
        1 caacgccacc ctctggccgc tgtaccacga cgcggtcgag ccacccgtct accgccgcag
       61 ctggtgggac aactaccgcg aggtcaaccg gcggttcgcc gcggagaccg cgaaggtggc
      121 cgccgagggc gccgtggtgt ggatccagga ctaccagctc cagctggtgc cggcgatgct
      181 ccgggagctg cggcccgacc tgcggatcgg cttcttcctg cacatcccgt tcccgccggt
      241 ggagctgttc atgcagatgc cgcggcgggt ggagatcatg caggggctgt tgggcgccga
      301 cctcgtcggc ttccagcgcc cgctcgccgc gcagaacttc ctgcggctga cccgccacct
      361 gctcggcctg cacccgaagg gcccggaggt acgggtcgac gatcgcaccg tgcgggtggc
      421 ggcgttcccg atctccatcg acgtgggcga gatcgagcgg ctcgcggcga gcgagtcagt
      481 ccgggcccgc tcggccgcga tccgcgagga gctgggcaac ccgaagcaca tcatcctggg
      541 tgtcgaccgg ctcgactaca ccaagggcat cgaccgccgg ctcaaggcgt tccgggaact
      601 gctgcaggag ggcaccctcg cggtgccgga cgtggtgatg gtgcaggtgg ccacgccgag
      661 ccgggagcgg gtggcgcact accagaccct gcgcgacgcg gtggagctgc aggtcggccg
      721 gatcaacggc gactacggca aggtcggggt gccggcggtg cactacctgc accagtcggt
      781 cgaccggacc gagctgaccg cgctgtactg cgcggctgac gtgatgatgg tgacgccgct
      841 gcgggacggc atgaacctcg tcgccaagga gtacgtggcg gcccgggtcg acctcggcgg
      901 atcgctggtg ctgtccgagt tcgccggggc cgcgtccgac ctgcgccagg cgtacctggt
      961 gaatccgcac gacaccgacg cgctcaagga cgggctggtg cgcgcggtcc gggtcgaccc
     1021 gaccgagtcg gcccgccgga tgcgggcgat gcggcggttc ctgcgcgggc acgacgtgga
     1081 ccactgggcc cggtcgttcc tcaccgcgct cggcgtaccg gaggctaccg acgcgctcgc
     1141 cgccgtggtg ccgccggcgc cgccgcccgg tcgtacccgc cggccggtcc gccgcgacgg
     1201 cggtcccgcc cgctgacgtg ccaccccgaa ggccagccga gcaccgacac cgcggaggca
     1261 gcatgagcac cgacgacgcg atcaccgcag cggaggcggc atgagcaccg acgacgcgat
     1321 caccgcagcg gaggcggcat gagtacggtg gcgccgaccg agccgttgga cgcggcgctg
     1381 cgcgagacgc tgggccggct ggcccgggta ccgcagctgc tggtggcggt ggactacgac
     1441 ggcacgctgg cgccgatcgt gggcgacccg gcggcggccc ggccgctgcc ggaggcggtg
     1501 accgcgctgc gcgcgctggc ggagctgccg cagacgaagg tcgcggtggt gtccggacgg
     1561 tcgctgcgcg acctggccgc cctgtcgcgg ctgcccagcg agatccacct ggtcggcagc
     1621 cacggctcgg agttcgacgt cgggttcgtg cagcagctgt ccgaggaggc ccggcagctg
     1681 cgtaccacgc tgcagcagca ggtacagcgg atcgccgccg gagcgccggg ggtccggctg
     1741 gaggcgaagc cggccagcat cgcggtgcac acccggaccg cgagcgacga ggtcgccgcg
     1801 agcgtgaacg cggcggtacg gtccggcccg gcgacgctgc ccggggtgca cgtgaccgag
     1861 ggcaagaagg tgatcgagct gtcggtggtg cacaccgaca agggcaccgc actggacacg
     1921 ttgcgcagcc ggctgggcgc ctcggcggtg ctgttcgtgg gcgacgacgt cacggacgag
     1981 aacggcttcg cccggctgcg cggcccggac gtcggggtga aggtcggctt cggcgacacg
     2041 cgggcgacgc accgggtggc cgatccgatc gaggtcgcga agctgctcgc gctggtgctc
     2101 gacatccgcc gccgctggct gtacggggag cgcgcggtgc cgatcgagcg gcactcgatg
     2161 ctcgccggcg gcgacaacct ggcgctggtg accccggacg cccggatcac ctggctgtgc
     2221 catccgcgac cggactcggc ggcgatcttc gccgacctgc tcggcggcag cccggccggg
     2281 tacttcggtg tcgcgccgca gcggggcggc ctgccgctgg ggcagaagta cctcggtggc
     2341 tcgatgaccg tgcagacgcg ctggtccggg ttgaccgtca ccgactggct cgattcgacg
     2401 gggccggcga accgcaccac cctggtccgg cagctgaccg gtaccgtcac ggtccgggtg
     2461 cagttcgcgc cgcggccgga gttcggccag gtggcggtgc ggctgcagcc gctgggcgac
     2521 gggctggtga tgctcgggtc caacgagccg gcggtgctct actcccccgg cgtcgactgg
     2581 gacatcgtcc aggagggcga gcacgacacc gcccacgcgc tgatcgacct gcgggccgcg
     2641 gggggcagcg cggtgctgga gctgcgctgc ggcaccgaca gcctggatcc ggcgccgtta
     2701 ccggtcgaac accgggccga cgagacggcg acgcagtggc ggcgctggtt ggacgggttg
     2761 cggctgccgg gggtgagcac cgagctggtg gcgcgcagcg cgctgacgct gcgcgcgctg
     2821 acctaccggc cgaccggggc gatcctcgct gccgggacgt ccagcctgcc ggaggatctg
     2881 ggcggggtgc gcaactggga ctaccggtac tgctggctgc gcgacgcggc gctggcggcc
     2941 cgggcgctgg tcgatctggg ttcggtcgac gaggcggagg ggctgctcgc ctggaccgcg
     3001 ggggtgatcg accgtaccgg cgggcatccg gagcggctgc acccgctgta cacggtggag
     3061 ggtcagacgc tcgggccgga ggcggtcatc gacacgctgc ccggttacgc cggctcccgg
     3121 ccggtgcggg tcggcaacgc ggcgaacaag cagctgcagc tcgacgtgtt cggcccgatc
     3181 gccgatctgg tcgacgcggt cgcgcagcgc cgcggcgtga tcagcgactt cgaccgcgag
     3241 gtgctgagcg cgatggtcga cgcggtggcg caccgctggt tcgagccgga ccacggcatc
     3301 tgggagcagc gccagccgcc ccggcaccac gtgtactcga aggtgatgtg ctggcagacg
     3361 gtcgaccgcg cgctgaacgt ggcggcccgg cacgggctgc cggagcggcc ggactgggtc
     3421 gagctgcgcg ccgcgatcgc cgagaacgtg ctggagtacg gctggaacga gcaggtcggc
     3481 gcctacacca ccgcgtacga cgggatcgac ctggacgcgg cctcgctgtg ggtggggctg
     3541 tccgggctgc tcgccgacga cgatccgcgg ttcctgtcca cggtgctcgc gatcgaggcg
     3601 gagctgcgca gtggcccggt ggtctaccgg taccgctcgg acgacgggat gcccggcacc
     3661 gagggcggct tccacgtgtg caccgcgtgg ctgatcgagt cgtacctgcg caccggtcgc
     3721 cgggccgacg ccgaggaact gtacgggcag ctgatcgcgt gcgccgggcc gaccgggctg
     3781 ctgcccgagc agtacgatcc gctcgccgag cggggcctcg gcaaccatcc gcaggcgtac
     3841 agccatctgg gcctgatccg ctgcgccctc ctactcgact cccgcccctg acccacccca
     3901 cctcaccccg ccccaggaag ttgatcaagg ccccaggaag ttgatcaagg gatccgcgca
     3961 cgacatcctg gacgttcgtg tacggatccc ttgatcaacg acgcggcctc ttgatcaacg
     4021 acgcggcccc ttgatcaacc gcgggggccg agccggggag cgcgcggagg ggcggcggcc
     4081 cgggggcgcg gtgggtcagc cgaccggggt gaagtcccgg ctggcgatga acggcgggcg
     4141 cggcgacggg gccgcgaacg gctccaccgt ggcgttctcc acgctgttga acaccacgaa
     4201 gatgttcgac cgcggccacg gcgtgatgtt gccgttggag ccgtgcatgc agttgcagtc
     4261 gaagatcgtc gccgacccgg ccggcccggt gaactgctcg atgccgtgct tgttcgcgag
     4321 cagcttgagg ctgtcctggt ccggggtgcc gatctcctgt tccttcagcg actgcttgta
     4381 gtgctccgcc ggcgtctcac cgacacagga gacgaacgtg cggtgcgagc ccggcatgat
     4441 catcaggcag ccgttctgcg cgaagttctc cgtcagcgcg atggagatgc tgcacgcccg
     4501 catcgccggc atgccgtcct cggcgtgcca ggtctcgaag tcggagtgcc agtagaagcc
     4561 ggcgccaccg aacccgggct tgtagttgat ccggctctgg tgcacgtaca cgtcggagcc
     4621 gaggatctgc cgcgcgcgac cgacgacccg ctcgtcggcg acgagcttcg ccaccagctc
     4681 ggagatcttg tgcacctcga agatcgagcg cacctcgttc gacgacttct ccaccacggt
     4741 gcgctcgtcc gcccgtaccg ccgggtcggt ggcgagccgg tccagctcgg cggagtacag
     4801 ctccacctcg gcgtcggtga gcagctggtc gacgctgagg tagccgttct tgtcgtagtc
     4861 ctccagggtc tgcgtgtcga tcggccccgt cgcaccggtc gaccagacca ccgggtcacg
     4921 gcggaagatc aggctcggcg tcggacgctt gcgcgtcggg tactcgtcgg gcacggtcgt
     4981 cggcagggtc acggtcgtgg tcatgcggac acctcctggg tggtctcttc ttcgacgatc
     5041 agcgggtaga caccgttgtc gtcgtgcacc tcacggccgg tcaccggcgg gttgaacacg
     5101 cagatcgtgc ggatgtcggt gatggggcgc atgatgtggt gctcgtgccc gtcgagcagg
     5161 tacatggtgc cgggcacgat gtggtgcttc tcgccggtct cggcgttggt cagctcggcc
     5221 tcgccctcga tgcacagcac cgcctcgatg tggttcgcgt accacatgtg cgtctcggtg
     5281 ccggcgtaca tcaccgtctc gtgcagcgag aagccgacct tctccttcgc cagcaccaac
     5341 cgcttgctgc gccaggtggc cgccttgacg tcgcggtcgg tctcggtgat gtcggcaagt
     5401 gaacgaacga tcattggtcc tccagctcag tgtgcggcgt cggcgaccgc gtcggtgagg
     5461 atctcgagtc cctcacccag ttcgtcgtgg ccgatcgtca acgcgggcat cagcttggcg
     5521 acctcgccgt ccgcaccggc cgtctcgatg agcaggccgc ggtcgaacgc gccggtcacc
     5581 accttcgccg ccacgtccgc gtcggcgaag gcgaggcccc aggcgagtcc ccgcccgcgt
     5641 accgcgatgt cgagctcggc gtgcttcgac gcgatggcgg tgagcgccga ctcgaccacc
     5701 tcgcccttcg tcgccgtctg ggccggcagc aggtcgtcgg tccagaacag ttcgagggcc
     5761 cgggccgcgg tcacgaacgc cggattgttg ccccggaacg taccgttgtg ctcgcccggt
     5821 gaccacacgt ccagttccgg ccgcagcagc accagcgcga gcggcagccc gtagccgccg
     5881 atcgacttgg acaggcagac gatgtccggc tcgatgccgg ccacctcgaa gctgaagaac
     5941 ggcccggtgc ggccgcaccc catctgcacg tcgtcgacga tgagcaggat gtcgtgccgt
     6001 ttgcacagct cggccaggga gcgcagccat tccggccggg ccacgttgat gccgccctcg
     6061 ccctgcacgg tctccacgat gaccgccgcc ggcgcgttca gcccgctgcc cgaatcggcc
     6121 agcagccgct cgaaccacag gaagtccggc acctggccgt ccagatagtt gtcgaacggc
     6181 atcggcgtgg cgtggaccag cggaatgccg gcaccgccgc gtttcatcgc gtttccggtg
     6241 accgacaatg aaccgagggt catcccgtgg aaaccatgtg tgaagctgat gatcgactcg
     6301 cgcccggtca ccttgcgggc cagcttcaac gccgcctcga ccgcgttggt accggtgggg
     6361 ccggggaact gcaccttgta gtccaggccg cgcggagcca gcacgtactc gcgcaaagcc
     6421 gccagcagat cgcgtttcgc ggtggtgtgc atgtccagcc cgtgcgtcac gccgtcacgc
     6481 tcgaggtagg cgagcaacgc ccgtttcagc tcgggatggt tgtggccata gttcagtgcg
     6541 cccgcgcctg cgaagaaatc aaggtagggg cgaccgttct cgtcgtacat tcggctaccg
     6601 acggcatggt cgaaagcgac cggccatgtc cggcagtaac tacggacttc ggattcgaga
     6661 tcggcgaaga gattcatcat cctccgtcgg ttgttcggtt gtggtcagcg tggcagcgga
     6721 ccgattcgga acaacacctc ctcgttgtgc gggccaccac tgaaatgctc ttcggtaaac
     6781 agggattgtt tggtgagcga ggcaccgcga gcgcgggcga atcccgcgaa cagacgtgcc
     6841 gagggcacgt tgtctggtgt caccgtggcc tccaggaaga ccacggactg cgtcctcgca
     6901 acccgatcga ccagcgcgtc cagcgcgcgt acggccaatc cacgccgccg gtatgcggcg
     6961 tcgaccgcca cctgccagac aaacagcgtg tccggcgcat cggggcgcag gtacccggtg
     7021 acgaacgagg ccaccgagtc accgacacag ccgaccaccg aggtggccgc gaaatcacgg
     7081 caccacagca gatagctgta ttcggagttg acgtcgagcg tgccggaatc ggccgccagc
     7141 cgccagagtc gggttccgtc ggcaaccgtc ggagcccgaa acacgaccga gccaccgcga
     7201 tcgtcggtca tcgactcgac ggtcgcgctt ggcgaatcct gcggttgccc ggtcatggct
     7261 gccgacgata gccggggcgc actgaccgct caaccggaac gggccacaga aggcccggat
     7321 tcccaatagc cacaacagat cccgagaaac tgtggcataa ttaacagaaa ataatccggc
     7381 aaaaactctg gccaggcgac ccgcccgacc ggtaacgtaa ctcagatccc ctgatgcacg
     7441 ctgcgctcct ccagcgccgc cgccggcggc cccggaaagt gccgccagcg gtgattcgaa
     7501 gccctgccag gagcgtggat cagccggcgc cgatggcgtc gacgaccggg ccgatcacca
     7561 cgaccgccgg ggcgccgatc ccctgccggt ccgccgccgc cgcaatcgtc gccagcgtcg
     7621 cccgcaccgt tctctgctgc ggcgtcccgc cgcgctccac cacggccgcc ggcgtgccgc
     7681 cggcccgacc gagctcgatc aaccgggccg cgatcgaggc caggttcgcc acggccatca
     7741 gcagcaccac ggtgccgcgc agccgggcca gcgccgccca gtcgaccagc gaacccggat
     7801 gctccggcgg gaggtgaccg gagcacacca ccagctcgtg cgtgaggccg cgatgggtga
     7861 ccgggatgcc ggccagcccc ggtaccgcga tcgcgctgga cacgccgggg accaccgtca
     7921 ccggcacacc ggccgcgagg caggccgccg cctcctcgcc gccacggccg aatacgaacg
     7981 ggtcgccgcc cttgagccgg acgacgaacc ggccggccag cgcgcgctcc accaggacct
     8041 cgttgatcga ctcctgggcg agctgacgcc cgcgcgggat cttcgccgcg tcgatcacct
     8101 cgacgtcgcc gcgcagcccg tccagcagca gctgcggcgc cagccggtcg accaccacga
     8161 cgttggcccg cgacagcagc cgccgaccac gcacggtgat cagttccgga tcgccgggcc
     8221 cggccccgac cagcgccacc ccgggcaccg ggcgcggcga cgccggtacc agccggcccg
     8281 cgggcgacgg ttccgggtgc cggaaccggg gggagtcgag gctaccgtcg gccaggccgg
     8341 cggcgatcgc ctcgcgcagc cgtaccgacc ggcgcgggtc gccgccaccc cacacggcga
     8401 cggtcaactc gtcggcaccg gtgacggtcg gcgtccaggc gctcgccgcc gccgcgtcgt
     8461 cggcccggac gcagaacacc cggcgttggg acgccgcatc cgacaccgcg gcgttcgcgg
     8521 ccgggtcatc ggtggcggcc agtaccagcc actgaccatc cacatcggac ggtgtgaacg
     8581 ccctttgctg ccaatggatc tcgccgaggg aggcacgctc gtccagagcc ggcgtcgcgg
     8641 ccggtgcgac cagcgtgacg accgcgtccg cggcgagcag ggcggggatc cggcgctgcg
     8701 cgaccgcgcc gccccccacc accaacaccg ggcggccagc aagccgcaga cccagcgggt
     8761 acagctcgct caccgggcac cgcccgccgc cgccagcgcc gcgacgaacc cgtcggtggt
     8821 accggcgtcc cggacggtga tgcgcagcca gtccgggccg aggccgggaa acgtgtcgcc
     8881 gcggcgcacc gcgtacccgg cggaacgaag cgcctcgcgc aaccgatccg cccccggccg
     8941 atggaccaac acgaagcgtg acctcggtac gccgaccacc ggcagctcgg cggcggccag
     9001 ccgggccatc aggtaccgcc ggtcggtgtc gagctgctcg gcgatgcgtc gctccgcggc
     9061 caccgcacgg ggctcggcaa gtacccgagc ggcggccagg gccggtgacg acaccggcca
     9121 cagcggctgt gccgcggcgc agcgggcgac cacgtcggcc gccccgagca ggtacccgac
     9181 ccgcagcccg gcgagccccc acgtcttggt gaagctgcgg atcaccacca ggccgggcag
     9241 gtcgcggcgg cccgccagcg actccggctc acccggcacc gtgtcggcga acgcctcgtc
     9301 gaccaccacg atgcgaccgg gccggcacag ccccgcgatc gtctccgccg ggtgcagtac
     9361 cgacgtcggg ttcgtcgggt tgccgatcat gaccaggtcg gcgtcgtccg gaaccagttc
     9421 cggcgccagc tcgaacggcg gcgccagcaa cacccggtcg acccggtggc cggccgagcg
     9481 cagcgccgcc tccggctcgg tgaactgcgg gtgtaccagc accgcgcggt gcaccggcag
     9541 cgcccgcgcc agcagcacga acgcctccgc ggcgccggcg gtgagcagta cctcggactc
     9601 cggccggccg tgccgggcgg ccaccgcgcc acgggccggg cccggatcgg gatagtgcgc
     9661 gaggtcgtcg accgccgccc ggaccgcgtc ggcgagccag cccggcgccg gctcgtgccg
     9721 gacgttgacg gcaagatcga cgaggccggg tgccagttcg gtgtcgccat ggtgactcag
     9781 atcgacggcg tcagccgcta ggctccgctc cggaagcgcg acaagacgct cacgcgacgc
     9841 cacgctgagt gttccgtccg gtgacgcagc ggtgcggtcc ccgcttgccg atgcggccgg
     9901 agacactgcc gcgggcgtgg cgtcgaccgc gccccccaga tccataggca tgacgccatt
     9961 ctcacaaccg aacgaacggc cgagtgtgtg cggcccagtc ttgccttaat gtgcctcgct
    10021 gggatttcgt gacccgcgat cccggcatgc cggccgcgca gcggccgggg cggagcagca
    10081 gcggtcccga gaaccgcagt ctcggcgtac ggggaggatt cagcgttgac gactccacag
    10141 agcggcccgc ccgacgaccg tcgcaccgcg acgccgccgg gcagtggtcg gcaccgttcg
    10201 gacctgccca tgcagccgcc gaacgcgccg accgcggccg gcccgccacc gaacgggttc
    10261 gccgcaccgc ccagctcgcc gggtccgtac ggctcgcccg gcccgtacgg accgcccggt
    10321 ggcggctacc cgccaccgaa cggcgccgcg ccgtacgtgc cgtcgacgcg ccccggcccg
    10381 cgcaacgagc cgttgccgcc cccacaagcc tcgg
//
