LOCUS       NZ_AP023354            10795 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Actinocatenispora sera strain NBRC 101916 chromosome.
ACCESSION   NZ_AP023354
VERSION     NZ_AP023354.1
KEYWORDS    RefSeq.
SOURCE      Actinocatenispora sera
  ORGANISM  Actinocatenispora sera
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinocatenispora.
REFERENCE   1
  AUTHORS   Komaki,H. and Tamura,T.
  TITLE     Whole genome shotgun sequence of Actinocatenispora sera NBRC 101916
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7044238)
  AUTHORS   Ichikawa,N., Komaki,H., Tamura,T. and Uohara,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2020) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :https://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4
            Genome Coverage                   :: 251x
            Sequencing Technology             :: SMRT cell 8 Pac V3
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018324685.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 04:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,585
            CDSs (total)                      :: 6,501
            Genes (coding)                    :: 6,404
            CDSs (with protein)               :: 6,404
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 26 of 97
            Pseudo Genes (incomplete)         :: 81 of 97
            Pseudo Genes (internal stop)      :: 1 of 97
            Pseudo Genes (multiple problems)  :: 10 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:48:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3763053
            Orig. end                         :: 3773848
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Annotated at DFAST https://dfast.nig.ac.jp/
FEATURES             Location/Qualifiers
     region          1..10795
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10795
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10795
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5795](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5795
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            449..1072
                     /locus_tag="Asera_RS17925"
                     /old_locus_tag="Asera_35820"
     CDS             449..1072
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Asera_RS17925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Asera_35820"
                     /product="hypothetical protein"
                     /protein_id="WP_051802615.1"
                     /transl_table=11
                     /translation="MGATRHRRPRAHLHPARWLGCRLRNGNPLRRGSDRAEGICALFAV
                     LLLLVAVPVTILIGLNIAASRSSAAARERAERQPATAVLLANAPDSIGVAAADGAAGSV
                     PVRARWHVGDQARTGTVYATPGLHRGAQVPIWLDHRGRPVAAPPSTTQVTMSSAGTGLG
                     LLFAIVAGVGAAWLVLRWLLDRARLAAWEREWRRVNPHGNHWVR"
     gene            complement(1135..3621)
                     /locus_tag="Asera_RS17930"
                     /old_locus_tag="Asera_35830"
     CDS             complement(1135..3621)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /locus_tag="Asera_RS17930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35830"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_211255671.1"
                     /transl_table=11
                     /translation="MPNAQPDPTGVTSPNAAPGRYEGTAASAVSAPADGFVRVFDAHEH
                     NLAHVDVAVPRDVLAVFTGVSGSGKSSLAFGTIYGEAQRRYLESVAPYARRLIQQAGVP
                     HVDEITGLPPAVALEQRRAAGSSRSSVGTLTTLSNLLRMLFSRAGDYPPGIAFMDSDSF
                     SPNTAVGACPRCHGLGTVHEVTEASLVPDPSLSIREHAIAAWPGAWLGKNLRDIVATLG
                     YDIDKPWRELDQADRDWILFTDDQPVVTVHPEREAGRIQRPYQGKYQSAKRYVLHTFAD
                     SKSATMRRRMERFLVSGPCPTCHGARLKPEALAVTYAGRTIAQLTGEPLSELAELLRLT
                     AEHGSPDHPAQPIAADLVARVDVLSKMGIGYLSMDRATPTLSAGELQRLRLATQLRSGL
                     FGVVYVLDEPSAGLHPADTEALLAVLDELRAAGNSLFVVEHDLDVVRRADWVVDVGPGP
                     GEYGGRILYSGPVSGLAGAAGSVTRRYLFDGAGERRTTRRDPSSWLTLTGVRRHNLADL
                     TVSLPLGVFTAITGLSGAGKSTLLQVLAEVVRAKLGDEPADDLPDADGLEPVGRLVQVD
                     QKPIGRTPRSNLATYTGLFDVVRKAFADTPQARELGYAAGRFSFNVPGGRCETCQGDGY
                     VAVELLFLPGTYAPCPTCHGARYTPETLEITYHGRNIAAVLDLSVDAATEFFADLPVAL
                     RSLRALRRVGLGYLRLGQPATELSGGEAQRIKLATELQRPARGHTIYLLDEPTTGLHPA
                     DIELLDAQLHELVDAGNTVVVVEQDLATIAGADWVIDLGPAGGHRGGHVVATGTPEQLT
                     TNATSPTAPYLRPHLH"
     gene            complement(3707..3853)
                     /locus_tag="Asera_RS17935"
     CDS             complement(3707..3853)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Asera_RS17935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157034992.1"
                     /transl_table=11
                     /translation="MTSEQDENASERSTGRHAAPDGAPRDPRIDLSRDPHPGVPDHAAP
                     EED"
     gene            4019..4996
                     /locus_tag="Asera_RS17940"
                     /old_locus_tag="Asera_35850"
     CDS             4019..4996
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515886.1"
                     /locus_tag="Asera_RS17940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35850"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_035297641.1"
                     /transl_table=11
                     /translation="MTQPQPVLDPLSPAALFLVLSVRPGSEAAVRDVLADVSGLVRSVG
                     FRSAHGELSCVVGVGSAIWDRLFGSPRPAKLHPFRPLRGPRHHAPATPGDLLLHIRAGR
                     YDLCYELGVLILDRLAGLVVVEDETMGFRYFEQRDLLGFVDGTENPTGIGAARAVLIGD
                     EDPDFAGGSYLIVQKYLHDMSAWTALSTEDQERVIGRTKLADVELPDDVQPSNSHVALN
                     TIVDADGTEHQIVRSNMPFGSPSRGESGTYFVGYAADPGVTELMLRRMFLGDPPGNYDR
                     ILDFSTAVTGSLFYIPTVDFLDDPPEPTGGLPDGSLAIGDMRGE"
     gene            5001..5795
                     /locus_tag="Asera_RS17945"
                     /old_locus_tag="Asera_35860"
     CDS             5001..5795
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083253.1"
                     /locus_tag="Asera_RS17945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35860"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_030447847.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.2e-93, bitscore:
                     311.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISDAAWDDLSQEARRTFTRHLAGRRVVDVSGPDGT
                     ELAAVGTGRRERIDAPDGVRAHRRVAQPLVELRVPFTISRDEVDAVERGAKDADWQPVK
                     DAAKRMAFAEDRAIFEGYAAAGIGGIRAGASNPALTLPGEVRDYPDTISRAVSELRLAG
                     VAGPYSLLLSADAYTQVNETSDHGYPIQEHLRRLLDGDIIWAPAISGAFLLSTRGGDFE
                     LHLGQDLSIGYTAHDADSIELYFIQSFTFLHYTTEAAVPISS"
     gene            complement(5918..6172)
                     /locus_tag="Asera_RS17950"
                     /old_locus_tag="Asera_35870"
     CDS             complement(5918..6172)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Asera_RS17950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Asera_35870"
                     /product="hypothetical protein"
                     /protein_id="WP_157034993.1"
                     /transl_table=11
                     /translation="MREPTAAYVRAAEEIRAGDQVFISPAAGVHGRGCWWAMVVSRMPA
                     LVQGAVYLRVVPVDAIGSDEVTPMVCYANLSGLLVRRAS"
     gene            6324..6569
                     /locus_tag="Asera_RS17955"
                     /old_locus_tag="Asera_35880"
     CDS             6324..6569
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455206.1"
                     /locus_tag="Asera_RS17955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35880"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_035297643.1"
                     /transl_table=11
                     /translation="MVDLSGLRPGAATPLYVQLRDVMKAAIEAGEYGAGTRLPSETDIA
                     QQTGVARLTVRKAYRLLAEEGLVRTIPQRGTYVEPA"
     gene            complement(6766..7446)
                     /locus_tag="Asera_RS17960"
                     /old_locus_tag="Asera_35890"
     CDS             complement(6766..7446)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Asera_RS17960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Asera_35890"
                     /product="hypothetical protein"
                     /protein_id="WP_030447850.1"
                     /transl_table=11
                     /translation="MTLPSDTPRLRCGIVLRVRQGDCEVLADDGPATVGYATPFPTPHV
                     ERVSPGNLVALATGPGGTEVVVWRWYDAIVLADDPDAVTLWEAAHGENRARRRSPSRRD
                     QPGTRAYLCAGLPGADWWVAGPAISDPKAADVELTEVARLYTEHDLGPTAFPPLTPGSI
                     AVRLAVAFRHAPRPVAPARISGSRTPTLAPRSAHPGRSIKPAPQTKVFFWFRARTRRQT
                     KDFF"
     gene            complement(7644..8348)
                     /locus_tag="Asera_RS17965"
                     /old_locus_tag="Asera_35900"
     CDS             complement(7644..8348)
                     /NRPS_PKS="Domain: PKS_KR (3-114). E-value: 1.4e-08. Score:
                     26.8. Matches aSDomain:
                     nrpspksdomains_Asera_RS17965_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078466.1"
                     /locus_tag="Asera_RS17965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35900"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_030447851.1"
                     /transl_table=11
                     /translation="MTITLVTGANRGLGYETARRLVEAGQTVYLGARDPERGAKAAAEL
                     GARYVQLDVTDDGSVRAALRTIADDAGGLDVLVNNAGIAELRGPSEADAVTGESALRQF
                     DTNAVGVVRVTTAALPLLRASANPVVVNVSSALGSFWAVTNPQRRQFHYPSIVYGATKA
                     AVSMLTVQYAKAVPEVKFNAVEPGFTATDFTAGIEGGRPVEQSAEVIVRLATIGPDGPT
                     GTFTEDDHPLPW"
     aSDomain        complement(8007..8339)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Asera_RS17965_PKS_KR.1"
                     /evalue="1.40E-08"
                     /label="Asera_RS17965_PKS_KR.1"
                     /locus_tag="Asera_RS17965"
                     /protein_end="114"
                     /protein_start="3"
                     /score="26.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TLVTGANRGLGYETARRLVEAGQTVYLGARDPERGAKAAAELGAR
                     YVQLDVTDDGSVRAALRTIADDAGGLDVLVNNAGIAELRGPSEADAVTGESALRQFDTN
                     AVGVVRV"
     CDS_motif       complement(8202..8336)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Asera_RS17965_0001"
                     /evalue="1.00E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="Asera_RS17965"
                     /protein_end="49"
                     /protein_start="4"
                     /score="18.2"
                     /tool="antismash"
                     /translation="LVTGANRGLGYETARRLVEAGQTVYLGARDPERGAKAAAELGARY
                     "
     gene            8544..9401
                     /locus_tag="Asera_RS17970"
                     /old_locus_tag="Asera_35910"
     CDS             8544..9401
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511962.1"
                     /locus_tag="Asera_RS17970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35910"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_030447852.1"
                     /transl_table=11
                     /translation="MTDDTNRLGEYLRARRELVSPDSLGLPQVGVRRVPGLRREEVAML
                     AGISADYYLRLEQGRDRNPSVQVLESLARVLQLDEAATGYLISLAAERPRRTRRRPRRE
                     SVPVGVRKLLDTLGLPAFVEGRYFDVLAANPLAATISPRFAVGHNRLLDVFLDPAEQAM
                     YPDWDRATAGLVAGFRQAVGTDTDDPRFIELVGELSLGSGRFRRLWARHDVGPWEGSAV
                     LLEHPQVGEITLYREKLAIGGTSGQVLAVYHADRGSADAEKLALLTSYQVETAAPAPTA
                     HQPD"
     gene            complement(9438..10649)
                     /locus_tag="Asera_RS17975"
                     /old_locus_tag="Asera_35920"
     CDS             complement(9438..10649)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /locus_tag="Asera_RS17975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Asera_35920"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_030447853.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.6e-06, bitscore:
                     25.4, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSASQPVSVLGLGRMGTALARRLADLGFPVTGWSRSGVALDGVR
                     AAGSPAAAVAGADVVLLALYDATACRQVLARCAASLRPGATVLNLATIAPAEATELHRD
                     TPVRYVQCPVLGSAPAAATGTLTLLYGGAAPAPAEAEVLAALGTVLGCGDAATAAAFKL
                     VANGALGDAIVALGQSLGRARRLGLGPEATLDVLARTALGGLVARKRDRLTGGAHAPAD
                     FTAGALGKDADLLAAVDPDSAVLPLLLASAGVPADADVAAIVIGLADTGHRVVDAAARL
                     YAAPGVPTDDELMAPLISYARGHASGDPAHFGAAFRPTAHVEGIRDDGFVSWDLDRYRA
                     NFTGTPAADEPARTRTVTELGRTGTVGHATMVLRHGSDTFTDLFVLVCEHGEWRIANKV
                     YHRC"
ORIGIN
        1 catcgcggcg cgctgcggcc ggtggtcctg ctcgggctcg gcaccaacgg cccgttcccg
       61 gccgacatgc tggagggcat catcggccag ctcggcccgg accggacgtt gtacctggtc
      121 aacgtgtacc tgccgacccg cccctggggc ggcgaggtca accacacgct gaccaaggtg
      181 gcctccgggc acaccaacgt gcacctgatc gactggcgca gcgccgccgg ggcccaccag
      241 aacctgctct ggccggaccg gatccacccg cggcccggcg ccggcgccga cctgtacgcc
      301 cggaccgtgg tcgaggcgat gagcacgccg atgcgcgccg cgccgcccgg ccgcgtccag
      361 tgaccgcgcc ggtccaacgg cgccacgaac gagagattcg gcttgataac cgcggtagag
      421 ccgtggccgg cggcgcatca tcggccgtat gggtgccacc cgtcaccgtc gcccccgggc
      481 ccacctccat ccggcccgct ggctgggctg caggctgcgc aacggcaatc cgctgcggcg
      541 cggctcggac cgcgccgagg gcatctgcgc gctgttcgcc gtcctgctgc tcctcgtcgc
      601 cgtaccggtg acgatcctga tcgggctgaa catcgcggcc agccgcagct cggccgccgc
      661 ccgagagcgg gccgagcgcc agccggccac cgcggtactg ctggccaacg cgcccgactc
      721 gatcggcgtc gcagcggccg acggcgccgc cggttccgtt ccggtccggg cccgctggca
      781 cgtcggtgac caggcccgca ccggtaccgt ctacgcgacg ccggggctgc atcgcggcgc
      841 ccaggtaccg atctggctcg accaccgcgg ccggcccgtc gcggcgccgc cgtccaccac
      901 ccaggtcacc atgtcgagtg ccggtaccgg gttggggctg ctgttcgcga tcgtggccgg
      961 cgtgggggcg gcgtggctgg tgctgcgctg gctgctggat cgggcccgat tggcggcgtg
     1021 ggagcgggag tggcgccggg tcaacccgca cggcaaccac tgggtgcggt gatcgttgcc
     1081 gggtcagcct gggcgcgcgt ggtgccgggc tggtgttgct ggccgggctg ccggtcagtg
     1141 gaggtggggg cgtaggtagg gggcggtcgg gctggtggcg ttggtggtga gctgttcggg
     1201 ggtgccggtg gcgacgacgt ggccgccccg gtggccgccg gccgggccga ggtcgatcac
     1261 ccagtcggcg ccggcgatcg tggcgaggtc ctgctcgacc acgaccaccg tgttgccggc
     1321 atcgaccagc tcgtgcagct gggcgtcgag cagttcgatg tcggccgggt gcagcccggt
     1381 ggtcggctcg tcgagcaggt agatcgtgtg cccgcgggcc ggtcgctgca gctcggtggc
     1441 gagcttgatc cgttgcgcct caccgccgga cagctcggtc gcgggctggc ccaaccgcag
     1501 gtaacccagt ccgacccggc gcagcgcgcg caggctgcgc aacgccaccg gcaggtcggc
     1561 gaagaactcg gtggccgcgt cgacggacag gtcgagtacc gcggcgatgt tccggccatg
     1621 gtaggtgatc tccagcgtct ccggggtgta gcgggcgccg tggcaggtcg ggcacggcgc
     1681 gtacgtgccg ggcaggaaca gcagctccac cgccacatac ccgtcgccct ggcaggtctc
     1741 gcaccggccg ccgggcacgt tgaaggagaa ccggcccgcg gcgtacccga gctcgcgcgc
     1801 ctgcggggtg tccgcgaacg ccttgcgtac cacgtcgaac aggccggtgt aggtggcgag
     1861 gttggaccgc ggggtgcggc cgatcggctt ctggtcgacc tgcaccaacc ggccgaccgg
     1921 ctcgagcccg tcggcgtcgg gcaggtcgtc cgccggttcg tcgccgagct tggcccgtac
     1981 cacctcggcg aggacctgca gcagcgtcga cttgccggca ccggagagcc cggtgatcgc
     2041 ggtgaacacg cccagcggca gggagacggt caggtctgcc agattgtgcc ggcgtacccc
     2101 ggtgagcgtc agccaggacg acgggtcgcg gcgcgtggtg cggcgctcgc cggccccgtc
     2161 gaacaggtac cgccgggtga ccgacccggc cgcgccggcg agcccggaaa cgggcccgga
     2221 gtaaaggatc cggccgccgt actcgccggg gccggggccg acgtccacca cccagtcggc
     2281 gcggcgcacc acgtcgaggt cgtgctcgac gacgaacagc gagttgccgg cggcgcgcag
     2341 ctcgtccagc accgcgagca gcgcctcggt gtccgcgggg tgcaggcccg ccgacggctc
     2401 gtccagcacg tacaccacac cgaacagccc ggatcgcagc tgggtggcca ggcgcagccg
     2461 ctgcaactca ccggcggaca gcgtcggggt ggcccgatcc atgctcaggt aaccgatccc
     2521 catcttcgac agcacgtcga cgcgggccac caggtcggcc gcgatcggct gggccgggtg
     2581 gtccggcgaa ccgtgttccg cggtgaggcg caacagttcc gccagctccg acagcggctc
     2641 gccggtcagc tgcgcgatgg tacggccggc gtacgtgacg gcgagcgcct cgggcttgag
     2701 ccgggcgccg tggcaggtcg ggcagggccc ggacaccagg aaacgctcca tccggcgccg
     2761 catcgtggcc gacttggagt ccgcgaacgt gtgcagcacg tagcgtttcg cgctctggta
     2821 cttgccctgg tacggccgct ggatccggcc cgcctcgcgc tccggatgca cggtgacgac
     2881 gggttggtcg tcggtgaaca ggatccagtc gcggtcggcc tgatccagct cgcgccacgg
     2941 cttgtcgatg tcgtagccga gggtggcgac gatgtcgcgc aggttcttgc ccagccaggc
     3001 gccgggccag gcggcgatcg cgtgctcccg gatcgacagc gacggatccg gtaccagcga
     3061 ggcctcggtg acctcgtgca ccgtacccag tccgtggcag cgggggcagg cgccgaccgc
     3121 ggtgttgggc gagaacgagt cggagtccat gaacgcgatc ccgggcgggt agtcgccggc
     3181 ccgggagaac agcatccgca acaggttgga cagcgtggtc agggtgccga ccgaggaccg
     3241 ggacgagccg gccgcccggc gctgctccag cgccaccgcc ggcggcagcc cggtgatctc
     3301 gtcgacgtgc ggcacacccg cctgctggat cagccgccgc gcgtacggcg cgaccgactc
     3361 caggtagcgc cgttgcgcct caccgtagat ggtgccgaac gccagcgacg acttgccgga
     3421 accggacacc ccggtgaaca ccgccagcac gtcccgcggt accgcgacgt cgacgtgcgc
     3481 caggttgtgc tcgtgggcgt cgaagacccg gacgaacccg tcggccggag cggacaccgc
     3541 gctggcggcg gtcccctcgt accggccggg cgccgcgttc gggctggtca ccccggtggg
     3601 atcgggctgt gcgttcggca tggacccagc atgaccgacg cgtccggggc ggtaccggca
     3661 gtccaccgca ccggcgcccc ggggccgggg taccggtgcc gggtgctcag tcctcttcgg
     3721 gcgcggcgtg gtccgggacc cccgggtgcg ggtcgcggct gaggtcgatc cgcgggtcgc
     3781 gcggcgcgcc gtcgggggcc gcgtgccggc cggtggagcg ctcgctcgcg ttctcgtcct
     3841 gctcggaagt cattcgaatc ctccccgtgt ccagctcatc gcgctgaacg tccgaatccc
     3901 tgagtcggta cgcgtatcgg acagcaaggt acacgagccg gggtgcgggc cagatcaccg
     3961 acacggaatc ggcggtttac ctaccggtag ctaccccgat cgtgcgatcc tgccggacat
     4021 gacgcagccg cagccggtac tcgatccact gtcgccggcg gcgctgttcc tggtgctgtc
     4081 ggtacggccg gggtccgagg cggcggtacg cgacgtgctc gccgacgtgt ccggcctggt
     4141 gcgttcggtc ggtttccggt ccgcgcacgg cgagctgagc tgcgtggtcg gggtcggctc
     4201 ggcgatctgg gaccggctgt tcggctcccc gcgaccggcg aagctgcacc cgttccggcc
     4261 gctgcgcggg ccccggcacc acgccccggc caccccgggt gacctgctgc tgcacatccg
     4321 cgccggccgc tacgacctgt gctacgagct gggtgtgctg atcctcgacc ggctcgccgg
     4381 cctggtcgtg gtcgaggacg agacgatggg cttccgttac ttcgagcagc gcgacctgct
     4441 cggcttcgtc gacggcacgg agaacccgac cgggatcggt gccgcccgcg ccgtgctgat
     4501 cggcgacgag gacccggact tcgccggcgg cagctacctg atcgtgcaga agtacctgca
     4561 cgacatgagc gcgtggaccg cactgtccac agaggaccag gagcgggtga tcggccggac
     4621 caagctcgcc gacgtcgagc tgcccgacga cgtgcagccg agcaactcgc acgtggcgct
     4681 caacacgatc gtcgacgcgg acggcaccga gcaccagatc gtccggtcca acatgccgtt
     4741 cggctccccg tcccgaggcg agtccggtac ctacttcgtc gggtacgcgg cggatcccgg
     4801 ggtcaccgag ctgatgctgc ggcggatgtt cctcggcgac ccgcccggca actacgaccg
     4861 gatcctcgac ttctccaccg cggtcaccgg cagcctgttc tacatcccga ccgtcgactt
     4921 cctggacgat ccgccggaac cgaccggcgg cctgccggac ggatcgctgg cgatcggcga
     4981 catgcgaggg gaatgagcga atgaacaacc tgcaccgcga actggccccg atctcggacg
     5041 ccgcctggga cgacctgtcg caggaggccc ggcgtacctt cacccggcac ctcgccggcc
     5101 gccgcgtcgt cgacgtgtcc gggcccgacg gcaccgaact cgccgctgtc ggtaccggtc
     5161 gccgcgagcg catcgacgcg cccgacgggg tgcgcgcgca ccgccgggtc gcgcagccgc
     5221 tggtcgagct gcgggtcccg ttcaccatct cgcgcgacga ggtcgacgcg gtcgagcgcg
     5281 gtgccaagga cgccgactgg cagccggtca aggacgccgc gaagcggatg gcgttcgccg
     5341 aggaccgcgc catcttcgag gggtacgccg ccgccggcat cggcggcatc cgtgccggtg
     5401 cctccaatcc cgccctgacg ctgcccgggg aggtacgcga ctaccccgac acgatcagcc
     5461 gcgcggtcag cgaactgcgc ctcgccggcg tcgccgggcc ctactcactg ctgctcagcg
     5521 cggatgccta cacccaggtc aacgagacct ccgaccacgg ctacccgatc caggagcacc
     5581 tgcgccggct cctcgacggc gacatcatct gggcgccggc gatcagcggc gcgttcctgc
     5641 tctccacccg cggcggcgat ttcgagctgc acctcggcca ggacctctcg atcggttaca
     5701 ccgcacacga tgccgactcg atcgagctct acttcatcca gtccttcacg ttcctgcact
     5761 acaccaccga agccgctgtc cccatctcca gctgaccgcc gcgagccgcg tcagtccatg
     5821 atggacggcc cggctcagtg cccacggtac gaccaggccg cgcccagcac gaacgcgacc
     5881 accagcgaga gcacccagcc gatcgcgatc ccgatggtca tgacgcgcgg cggaccagga
     5941 ggcccgagag gttggcgtag cacaccatcg gggtcacctc gtcggacccg atcgcgtcga
     6001 ccggcacgac ccgcaggtac accgcgccct ggacgagggc gggcatccgt gacacgacca
     6061 tggcccacca acatccgcgc ccgtgcacgc cggccgccgg cgagatgaac acctgatcgc
     6121 ccgccctgat ctcctccgcc gcccgcacgt aggccgctgt cggttctcgc atggctgggt
     6181 cctccgtctc ggccggcacg tgacggccct gttcacgact gctcacgggt ctgaccatac
     6241 gcctcgtaga gacgtctggg aactagagga gacgtctaga gattgcccgg agcgctgccg
     6301 cctgcttggt tagcgtcggc accatggtgg atctcagcgg gctccggccg ggcgcggcga
     6361 ctccgctgta cgtgcagcta cgcgatgtga tgaaggcagc catcgaggcc ggtgagtacg
     6421 gtgccggtac ccgtctaccc tccgagaccg acatcgcgca gcagaccggt gtcgctcggc
     6481 tgacggtccg caaggcgtac cggctgctgg ccgaggaggg cctggtacgc accatcccgc
     6541 aacgtggcac ctatgtcgaa cctgcctgac ccccgccgcg cgcgatcacc agtgagaagg
     6601 tccaaccgag accgctgacg gggttcattc gcctgccgcc tgacctcgag ccagaggtcg
     6661 ggacgtagta ggtcttcggc tcgcctggtt cgtcgatcgg atttaccgtg taggcggccg
     6721 tggtccagga cgagcgggaa gcgcccgatg cgcgtcgacg tcaaactaaa agaaatcttt
     6781 agtttggcgt cgagtgcgcg cgcgaaacca aaagaaaact ttagtttggg gcgctggttt
     6841 gatcgagcgg cccgggtgcg ccgaccgcgg ggcgagcgtg ggagtcctag aacccgagat
     6901 gcgcgcgggg gcaacgggtc ggggtgcgtg gcggaaggcg acggcgagcc ggaccgcgat
     6961 cgagccaggg gtcagtgggg ggaaggcggt aggcccgagg tcgtgctcgg tgtacaggcg
     7021 ggcgacctcg gtcagctcga cgtccgcggc cttcggatcg ctgatcgcgg ggccggcgac
     7081 ccaccagtcg gcgccgggca gcccggcgca gaggtacgcg cgggtccccg gctggtcgcg
     7141 ccgggacggc gagcggcgtc gggcgcggtt ctccccgtgg gcggcttccc acagggtcac
     7201 ggcgtcggga tcgtcggcga gcacgatcgc gtcgtaccag cgccacacga cgacttcggt
     7261 accgccaggg cctgtcgcca gcgcgaccag gttgccgggg gagacccgtt cgacgtgcgg
     7321 ggtggggaac ggggtcgcgt agccgaccgt cgcgggcccg tcgtccgcga ggacctcgca
     7381 gtcgccttgc cgtacccgga gcacgatccc gcaacgcagc cgtggtgtgt cagaaggcag
     7441 ggtcatcgcg cgaggctacc gagctgccgg caccccgtcg atcgcgggtc gaggcgaagc
     7501 gccgcatccg cgccgatcga gaactggcgg ccgtcccctc ccaccgccga tcgcggactg
     7561 gtcgccggcg tgccggcgac cgaggactgg cggccggcgc gagacctcga tcggcgggtg
     7621 ggcgcggtcg ggtggtgtcc gggtcaccag gggagggggt ggtcgtcctc ggtgaaggtg
     7681 ccggtggggc cgtcggggcc gatggtggcg aggcggacga tgacctcggc gctctgctcg
     7741 accgggcggc cgccctcgat cccggcggtg aagtcggtgg cggtgaagcc gggctcgacg
     7801 gcgttgaact tcacctccgg taccgccttc gcgtactgca cggtgagcat cgagacggcg
     7861 gccttggttg ccccgtacac gatcgacggg tagtggaact ggcggcgctg cgggttggtg
     7921 accgcccaga acgagccgag cgcgctggac acgttgacca cgaccgggtt ggcggaggcg
     7981 cgcagcagcg gcagcgcggc cgtggtgacc cggacgacgc cgacggcgtt ggtgtcgaac
     8041 tggcgcagcg cggactcgcc ggtgaccgcg tcggcctcgg acgggccacg cagctcggcg
     8101 atgccggcgt tgttgacgag cacgtccagc ccgccggcgt cgtcggcgat ggtgcgcagg
     8161 gcggcgcgta ccgagccgtc gtcggtgacg tcgagctgca cgtaccgggc gccgagctcg
     8221 gccgccgcct tcgcgccacg ttcggggtcg cgggcaccga gatacacggt ctgtccggcc
     8281 tcgaccagcc ggcgggcggt ttcgtacccg agtccccggt tcgcgccggt gacgagcgtg
     8341 atggtcatgt cggtactcct tcgaacgcgg atgcacggcc ggtgtctggc cgggcggggc
     8401 cggtggccgc cggctgccgg acgaccgggt ctggacgtcg gcgagcccag cgtgcgtccc
     8461 cggcggcgcg cgagacagcg ccccgatgat cctgggggtg ccaggaccag ccaggacgcc
     8521 gccgggctgc ctacgctgaa gtcatgaccg acgacacgaa ccggctcggc gagtacctgc
     8581 gcgcgcggcg cgaactggtc tcgccggaca gcctcgggct gccccaggtc ggcgtgcgcc
     8641 gggtgcccgg gctgcgccgc gaggaggtgg cgatgctcgc cggcatcagc gccgactact
     8701 acctgcggct ggagcagggc cgcgaccgca acccgtcggt gcaggtgctg gagtcgctcg
     8761 cccgggtgct gcagctcgac gaggcggcga ccgggtacct gatcagcctg gcggcggagc
     8821 ggccgcggcg gacacgccgg cggccccgcc gggagagcgt gcccgtcggc gtgcgcaagc
     8881 tgctcgacac gctggggctg ccggcgttcg tcgagggccg ctacttcgac gtgttggcgg
     8941 cgaacccgct cgcggcgacg atctcgccac ggttcgcggt cggtcacaac cggctgctcg
     9001 acgtcttcct cgacccggcc gagcaggcga tgtacccgga ctgggaccgc gccaccgcgg
     9061 gcctggtcgc cgggttccgg caggcggtcg gtaccgacac cgacgacccg cggttcatcg
     9121 aactcgtcgg tgagctgtcg ctgggcagcg gccggttccg gcggctgtgg gcccggcacg
     9181 acgtgggccc gtgggagggt tccgcggtgc tgctggagca tccacaggtc ggcgagatca
     9241 ccctgtaccg ggagaagctc gcgatcggcg gtaccagcgg ccaggtgctc gctgtctacc
     9301 acgccgaccg gggcagcgcg gacgcggaga agctggccct gctcacctcg taccaggtgg
     9361 agaccgccgc gccggcgccg accgcccacc agccggactg accgcgcccg gacgggcgcg
     9421 aagcggggcc cggcgggtca gcagcggtgg tagaccttgt tcgcgatgcg ccactcgccg
     9481 tgctcgcaca ccaggacgaa caggtcggtg aaggtgtcgg acccgtggcg tagcaccatc
     9541 gtggcgtgcc cgacggtgcc ggtgcggccc agctcggtga cggtacgcgt ccgggccggc
     9601 tcgtccgccg ccggcgtccc ggtgaagttc gcccggtagc ggtcgaggtc ccaggagacg
     9661 aacccgtcgt cgcggatccc ctccacgtgc gcggtcggcc ggaacgcggc gccgaagtgc
     9721 gcggggtcgc cgctcgcgtg gccgcgcgcg tagctgatca gcggcgccat cagctcgtcg
     9781 tcggtgggga cgccgggtgc ggcgtacagg cgggcggcgg cgtcgacgac ccggtggccg
     9841 gtgtccgcca gcccgatgac gatcgcggcg acatcggcgt cggccggtac gccggcggac
     9901 gccagcagca ggggaagtac ggcgctgtcc gggtcgacgg ccgcgagcag gtcggcgtcc
     9961 ttgccgagcg cgccggcggt gaagtcggcg ggagcgtgcg cgccaccggt gagccggtcg
    10021 cgcttgcggg ccaccaggcc gcccagggcc gtgcgcgcca acacatccag cgtggcctcg
    10081 gggcccaggc cgagccggcg cgcgcggccc aacgactggc cgagcgcgac gatcgcgtcg
    10141 ccgagcgcgc cgttcgcgac cagcttgaac gccgcggcgg tggccgcgtc gccgcagccg
    10201 agcacggtgc ccagagcggc cagcacctcg gcctcggcgg gcgccggtgc ggccccgccg
    10261 tacagcaggg tgagcgtgcc ggtcgccgcg gccggcgcgg atccgagcac cgggcactgc
    10321 acgtaccgga ccggcgtgtc gcggtgcagt tcggtggcct cggccggggc gatggttgcc
    10381 aggttgagca ccgtggcgcc gggccgcagg ctcgcggcgc agcgggcgag cacctgccgg
    10441 caggcggtcg cgtcgtacag ggcgagcagg acgacgtcgg cgccggcgac cgcggcggcc
    10501 ggcgagccgg cggcccgcac gccgtcgagc gcgacgccgg accgggacca gccggtcacc
    10561 gggaagccga gatcggcgag cctgcgggcg agcgcggtgc ccatccggcc gaggccgagg
    10621 accgacaccg gctgcgaagc ggagttcatg ggcccagtct ggcgccagcg cagccatgcg
    10681 accagtgcag gattagcatg gcagccatgc ctgtcacgca tgctcggccg acgtcgctgg
    10741 cgccctgggt cgcgttcgtc gaggcgtgcc gggccggcag cctgtccgcg gtggc
//
