LOCUS       NZ_AP023356            21179 bp    DNA     circular CON 27-APR-2024
DEFINITION  Actinoplanes ianthinogenes strain NBRC 13996 chromosome, complete
            genome.
ACCESSION   NZ_AP023356
VERSION     NZ_AP023356.1
KEYWORDS    RefSeq.
SOURCE      Actinoplanes ianthinogenes
  ORGANISM  Actinoplanes ianthinogenes
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinoplanes.
REFERENCE   1
  AUTHORS   Komaki,H. and Tamura,T.
  TITLE     Whole genome shotgun sequence of Actinoplanes ianthinogenes NBRC
            13996
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9897806)
  AUTHORS   Ichikawa,N., Komaki,H., Tamura,T. and Uohara,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2020) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :https://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4
            Genome Coverage                   :: 168x
            Sequencing Technology             :: SMRT cell 8 Pac V3
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018324205.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,101
            CDSs (total)                      :: 8,999
            Genes (coding)                    :: 8,910
            CDSs (with protein)               :: 8,910
            Genes (RNA)                       :: 102
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 16 of 89
            Pseudo Genes (incomplete)         :: 75 of 89
            Pseudo Genes (internal stop)      :: 7 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:15:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6944986
            Orig. end                         :: 6966165
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023356.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21179
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="11"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21179
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21179
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11179](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11179
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(4..18)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            1599..2105
                     /locus_tag="Aiant_RS31620"
                     /old_locus_tag="Aiant_63820"
     CDS             1599..2105
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688984.1"
                     /locus_tag="Aiant_RS31620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63820"
                     /product="FBP domain-containing protein"
                     /protein_id="WP_189333710.1"
                     /transl_table=11
                     /translation="MEPISEATIRASFINGSKGEAARIKLPADFRDTPWSDLDFYGWVD
                     PAAPQRAALLLPREDRPVAVLLRKAERAGAGSAARSSMCQVCLTDHAAGGVSLFTAPLA
                     GAAGRNGNSVGEYLCADLACSLYLRGKKRPRLRLVRFEETLTLAEKIDRALTKLSAFTA
                     RIAAA"
     gene            complement(2325..3122)
                     /locus_tag="Aiant_RS31625"
                     /old_locus_tag="Aiant_63830"
     CDS             complement(2325..3122)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /locus_tag="Aiant_RS31625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63830"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_189333709.1"
                     /transl_table=11
                     /translation="MKKYRFYLASMGAAFGLYALLTALIAVHFFIDTDIAVRDGIARDL
                     PHWLISASKAAARLGQGGPLMYLSLVIAAAQSFRLKTVKHFALWACTSASLIMIVGSVK
                     MYTRRGAPADPQPGAVEFFSKEPCGGPACQSFPSGHVANSVVWYGLILILLNHWASSQA
                     RRTILASAVSVTAVANVVSGHHWLSDSLGGMLAGSGILGFLLIADRANPRIWGWLDKRI
                     FTSRRSGTTRPFREIGGMRPERSSGSPRGWQSPAQRTAPAGPH"
     gene            complement(3377..4933)
                     /locus_tag="Aiant_RS31630"
                     /old_locus_tag="Aiant_63840"
     CDS             complement(3377..4933)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442527.1"
                     /locus_tag="Aiant_RS31630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63840"
                     /product="sodium:proton antiporter"
                     /protein_id="WP_189333708.1"
                     /transl_table=11
                     /translation="MNGVQLLLIIGVGIAVTSVAHRHRFEPGWVIVVLAAAASFIPGVP
                     RLELESHLILAIVIPPLLWSATRGASVSGFGANLRPIVSLGVVLVVLTAGVLGWLSSLL
                     LPSIGVAAAFVLGSVLAPPDTITTVSHGDEIGLPKRVTSILTGESLVNDATALTLFSIA
                     VAAVNGEHATWTGGIGEFLRTATLGVLIGGAFAVLGLTVRKRLGNPTLETSLSLLLPFT
                     AFLTAEEVHASGILAVVAAAFLMSANLTLDPRHQYPGGYRTRLQEEQVWRVADFLLETF
                     VFAYIGLQLRFVLDDLADSAEPGLTRTLIAAGVLLAAAIAARLIGVFGLFGRWTLKQRM
                     IDRRIARDPRAARVHAGRVARRRGGEPLPPPTMKENALVGWTGMRGILTLAAAAAIPEH
                     TASGAPFPGRDAIQAIALIVTLGTLLLQGTTVGWLARRLRFDLTAEHAAAEELRTEGHR
                     IVAEAAAAPAPDVDASFEAQRLALGRAVMERRITEELARELIEDIDLRQAARHTNPADS
                     H"
     gene            complement(4953..5531)
                     /locus_tag="Aiant_RS31635"
                     /old_locus_tag="Aiant_63850"
     CDS             complement(4953..5531)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441885.1"
                     /locus_tag="Aiant_RS31635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63850"
                     /product="hypothetical protein"
                     /protein_id="WP_229830779.1"
                     /transl_table=11
                     /translation="MERPMTPDDVVAVIMGRLAEQHKVVPGYVIRDAVEAALRRRFPDV
                     PPAELWVPAEVAAELVSGFGDGAAVAEDDSATMPAVLSGDETGRLLAGLGLAVHVATFN
                     LDRDRLHVAQILNGSAAVLVALGAASRAAHADGTSAVLLSPATLRTVRTTMIGVLQGVR
                     TRGWQAEHLDPVATVTMFTDVLTMLGAVP"
     gene            complement(5551..6756)
                     /locus_tag="Aiant_RS31640"
                     /old_locus_tag="Aiant_63860"
     CDS             complement(5551..6756)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688989.1"
                     /locus_tag="Aiant_RS31640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63860"
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="WP_189333707.1"
                     /transl_table=11
                     /translation="MSDEVSDEVHDEARLAALHSYRVLDEPRAAVLDDLTRLAATMFDT
                     PMAAVSLIDRDRQWFAGSIGLADAQTPLDVSFCAQILPARRPLIVPDATAHPLFSGYRN
                     VVEAPNIRFYAGAPVIDEDGHVLGAMCVIDDVPREVSDRQVEALTTFAGQAATHLSAIR
                     NRLRMADLGDHLSRAAQREDDLVATITHELRTPVTSIQGYLELLSENEELAAYKRLIEP
                     IHRNGQRLVAMVDHILSGTRPHDAPLPAPVGPVDLNTVVAAALTSCRALTALRAAPVDV
                     FSGPPIVVQADLARLSHAIEQVLRNALLFTPADRPITIRVGAEPRPTVEVIDCGVGIPD
                     DELAFVFDRFYRGRHAREQAIPGVGLGLTIARASIAAHLGDLTVTSGPAGTTARVTLPA
                     VA"
     gene            complement(6805..7305)
                     /locus_tag="Aiant_RS31645"
                     /old_locus_tag="Aiant_63870"
     CDS             complement(6805..7305)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Aiant_RS31645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Aiant_63870"
                     /product="hypothetical protein"
                     /protein_id="WP_189333706.1"
                     /transl_table=11
                     /translation="MRTPVACTTVTILMFAGACSDPPSGSATYGPATSAPAPTGAAVSS
                     AECNAGISTEALPVWAREGFNFDGSGVPHRLGDQGGLMAILFAPPKADPVDGTKILWVS
                     RLPVETAAPLTVSGVRSDGLTATQELPNGAGPSAVKIPEPGCWDLTLTWSGHTDRMRLW
                     FTG"
     gene            complement(7416..8393)
                     /locus_tag="Aiant_RS31650"
                     /old_locus_tag="Aiant_63880"
     CDS             complement(7416..8393)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Aiant_RS31650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Aiant_63880"
                     /product="hypothetical protein"
                     /protein_id="WP_189333705.1"
                     /transl_table=11
                     /translation="MFRALLLTTASLTAGVLAAPGAALAADTSTSLTAAEMSAALKAVA
                     ATTATAEIPGYAGSLRATLDGRVAAMAFAVDPATGRGYYRMAYTGYGTEAVYAVAGKGE
                     YDYLTGSAERAAAKMAGRPEARYVFVPNAKLTLDGWSSRQMSAPSEIVVEDARHAGTKT
                     THDDGSADYSFTGEEKTRVTFTVDATGVVTRGRVVDTGVEQTITFGYGPQTVTLPATGQ
                     VISQTTLTKAVAYYDLARKMSRLTSDIMIGVDDRARGRNVTVAAVRKGARAAVAGFNES
                     LAVKVVTVRNVTGGVTISATNPYTGVRIAYRLKLSGKHTVARKL"
     misc_feature    8541..8554
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.92"
                     /tool="antismash"
     gene            8592..10001
                     /locus_tag="Aiant_RS31655"
                     /old_locus_tag="Aiant_63890"
     CDS             8592..10001
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688991.1"
                     /locus_tag="Aiant_RS31655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63890"
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_189333704.1"
                     /transl_table=11
                     /translation="MATPTQSEIRDDEQEQRRQSLSTAAARNLTTTTKTVPQMQEISSR
                     WLLRKLPWVQVSGGAFRVNRRMTYRIGDGRLSFTNVGAQVRVIPGELRELSVLSEFDEA
                     EVLAAMADKFVQQEYQPGQVIVEFGSVADHVYLIAHGKVNKVGVGNYGDPVNLGVLADG
                     EAFGEYSLTDDQRIWDYTAKAMTAVTLLAMPRSAFTALLGQSDRLRAHVDRFRAKNRRP
                     QNKHGEAAISVAAGHTGEPQLDGTYVDYEQSPREYELSVAQTILRVHTRVADLYNDPMN
                     QLEQQLRLTVEALRERQEYEIVNNREFGLLHNADLRQRIHTRSGPPTPDDLDELLSMRR
                     ATKMFVAHPQAIAAFGRECTKRGIYPPMVEHDGGTFLSWRGVPILPCGKIPVTETHTTS
                     ILAMRTGEADQGVVGLHQTGIPDEYQPSLSVRFMGIDEQAIMSYLVSAYYSAAVLVPDA
                     LGILDHVELSH"
     gene            10001..11179
                     /locus_tag="Aiant_RS31660"
                     /old_locus_tag="Aiant_63900"
     CDS             10001..11179
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688992.1"
                     /locus_tag="Aiant_RS31660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63900"
                     /product="family 2 encapsulin nanocompartment cargo protein
                     terpene cyclase"
                     /protein_id="WP_229830777.1"
                     /sec_met_domain="T1TS (E-value: 2.7e-53, bitscore: 180.9,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLTLDLGREILRDLRAAPSGDLAAVLGAVPGTGGPMTSGPAPDG
                     VPAMIGLGPSGLGTSAARAFRPVPARPDGPHLRCPGPLRDDPALGERVNEAVVRWAEGI
                     GIYPGRLDTLRSANFGRFMMLAHPATSDPDRLLAATKCLVAEWAADDYYVDEVSLGADP
                     MVVGSRLANLHAVVDPVSMVPRYLADYRDHHRLLPISRAFRSAMEHFAEYASVAQLGRF
                     QHQMAILFLAWTQEADWHANRRTPPVWEYLVQRHLNSYLPPMILIDVLGGYELPADEFY
                     HPRVRRAFTTAANAAVLINDLYSGKNESENDHNLPTVLAANEGLGHRAAVLRTVEIHNE
                     LMDDFVRLAGSLSVTGSPQLRRFLADTWAWLGGSREWHATSGRYHDSQEEKS"
     gene            11176..12018
                     /locus_tag="Aiant_RS31665"
                     /old_locus_tag="Aiant_63910"
     CDS             11176..12018
                     /EC_number="2.1.1.255"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0042214 - terpene metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688993.1"
                     /locus_tag="Aiant_RS31665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63910"
                     /product="geranyl diphosphate 2-C-methyltransferase"
                     /protein_id="WP_189333703.1"
                     /transl_table=11
                     /translation="MTTTAVSPLRTDFERSVANYWNTNRVDPVNLRLGEVDGLYHHHYG
                     VGEPDWSVLDGPEPGVIAELHRLESAQADLLLDHLGPIRPEDALLDGGSGRGGTSIMAN
                     ARFGCRVDGVSISEYQVGFANEQAARRGVADRVRFHFRNMLDSGFADGSRQAIWTNETT
                     MYVDLFDLYAEFARMLRFGGRYVCITGCANDVTGRRSKSVSKINEHYTCDIHPRSDYFK
                     ALAANDLVPINVVDLTAATIPYWELRAKSEVATGIEEAFLTAYREGSFHYLLIAADRV"
     gene            12103..13902
                     /locus_tag="Aiant_RS31670"
                     /old_locus_tag="Aiant_63920"
     CDS             12103..13902
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688994.1"
                     /locus_tag="Aiant_RS31670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63920"
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="WP_189333702.1"
                     /transl_table=11
                     /translation="MALPIEDYAIIADTQTAALVGRNGSIDWLCVPRFDSGAIFAALLG
                     SADNGHWTVAPTDVITTRRRYRDETLVLETEFETAGGVARLIDFMPPRTDSPSVIRIVE
                     GVRGQVDFSMELRLRFDYGHVVPWVYREGDALVGVAGPDAAWLRTPVDLRGENLTTKAD
                     FSVRAGERVPFVLTWRPSHLPPPEPLDPAHELGVTEGYWRGWISACTYEGEWRDAVVRS
                     LLTLKALTYAPTGGIVAAATTSLPEKLGGVRNWDYRFCWLRDATITLQALLFSGFQSEA
                     TAWRKWLLRAIAGNPAELQIMYGVGGERRLDEYLADWLGGYDGNPVRIGNAAAEQFQLD
                     VYGEVMDALHQGRRAGLKADDPAWGLQVKLMEFVENHWQDPDEGIWEVRGGPKQFTHSK
                     LMAWVAADRAVKAVEDFGLDGPLERWTELRSRIREDILEHGYDPDRKTFTQYYGSAELD
                     AAMLMVPLVGFLPADDERVAGTVAAIEKHLLVDGFVQRYTQHPDTDVDGLPPGEGAFLA
                     CTFWLADNYALMGRHDEARETFARLLALRNDVGLLSEEYDTVAGRLVGNFPQAFSHVPL
                     IDTARTLSSALGPTEARVREGLK"
     gene            complement(14121..14258)
                     /locus_tag="Aiant_RS31675"
     CDS             complement(14121..14258)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Aiant_RS31675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_212846553.1"
                     /transl_table=11
                     /translation="MDPVHGGGYGFGVRVAEAPQFLSMSATQTEFPASLLLTAEFVLLR
                     "
     gene            16013..16999
                     /locus_tag="Aiant_RS31680"
                     /old_locus_tag="Aiant_63940"
     CDS             16013..16999
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688996.1"
                     /locus_tag="Aiant_RS31680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63940"
                     /product="aldo/keto reductase"
                     /protein_id="WP_189333700.1"
                     /transl_table=11
                     /translation="MRERKLGDLTVSAIGFGAMGMSHGYGPGPDRDANIGLLRAAVERG
                     VTLFDTAEVYGPWVNEELVGEALEPFKGQVVIATKFGFVIDDDGRQGAGVNSRPENIRK
                     VAEASLRRLRVDAIDLFYQHRVDPDVPIEDVAGTVRDLIAAGKVRHFGMSEAAPGTIRR
                     AHAVQPVTAVQSEYSLWWRRPEEELLDTLAELGIGFVPFSPLGRGFLTGKIGADTVFAE
                     NDMRNGLPRFSAAAREANQALVELIGRIAAGKAATPAQVALAWLLAQRPWIVPIPGTRR
                     LDRLDENLGAAGIELTAADLAEIETAAAKIEVQGSRYPEQMEKMTNL"
     gene            complement(16996..18219)
                     /locus_tag="Aiant_RS31685"
                     /old_locus_tag="Aiant_63950"
     CDS             complement(16996..18219)
                     /EC_number="2.6.1.9"
                     /GO_function="GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (50-361). E-value:
                     7.2e-32. Score: 102.8. Matches aSDomain:
                     nrpspksdomains_Aiant_RS31685_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441888.1"
                     /locus_tag="Aiant_RS31685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63950"
                     /product="histidinol-phosphate transaminase"
                     /protein_id="WP_189333699.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 9.4e-30, bitscore:
                     103.0, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTGIDTAILHKGAHSASYFDLARAAGEGAEIVDFCIPCNPYFP
                     TPEMFDELTRELKSILKYYPSDSATITKKLAAVLNLHPQTVAMANGSTELITWIDHLLV
                     KESLAIPIPTFGRWTDQPLETGKRVDMFPLQERDGFRLDLEEYVEFIRERRSRVAVVCN
                     VNNPDGNYLPRRDVIRFMDQLSDLDLVVIDESFIDFSDAERYPSVGPDAVIRPNTVVLK
                     SLGKNFGLHGIRFGYMLANPAIAGKIAKMLPKWNLNSLAEKVVHMIEQHEMEYEESLRL
                     LSRDRRSMGRELQRIPGLTVFPSQGNFFLVKLPTEWSGVALRDFLVANHGIMTRECGNK
                     LGMTSQFMRLVVRPAGDVDRLVDGMIDYSQRFHGRSVYDTDPLESSSRRWTEQYEEKEY
                     APRRAAVG"
     aSDomain        complement(17137..18069)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Aiant_RS31685_Aminotran_1_2.1"
                     /evalue="7.20E-32"
                     /label="Aiant_RS31685_Aminotran_1_2.1"
                     /locus_tag="Aiant_RS31685"
                     /protein_end="361"
                     /protein_start="50"
                     /score="102.8"
                     /tool="antismash"
                     /translation="DELTRELKSILKYYPSDSATITKKLAAVLNLHPQTVAMANGSTEL
                     ITWIDHLLVKESLAIPIPTFGRWTDQPLETGKRVDMFPLQERDGFRLDLEEYVEFIRER
                     RSRVAVVCNVNNPDGNYLPRRDVIRFMDQLSDLDLVVIDESFIDFSDAERYPSVGPDAV
                     IRPNTVVLKSLGKNFGLHGIRFGYMLANPAIAGKIAKMLPKWNLNSLAEKVVHMIEQHE
                     MEYEESLRLLSRDRRSMGRELQRIPGLTVFPSQGNFFLVKLPTEWSGVALRDFLVANHG
                     IMTRECGNKLGMTSQFMRLVVRPAGDVDRL"
     gene            18348..18518
                     /locus_tag="Aiant_RS31690"
                     /old_locus_tag="Aiant_63960"
     CDS             18348..18518
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="Aiant_RS31690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Aiant_63960"
                     /product="hypothetical protein"
                     /protein_id="WP_189333698.1"
                     /transl_table=11
                     /translation="MRTGLIGCVVLGSVTVADEARARPGGGEPVRAVQWTVDSGARRHP
                     VPVPGDVRKGC"
     gene            18577..19062
                     /locus_tag="Aiant_RS31695"
                     /old_locus_tag="Aiant_63970"
     CDS             18577..19062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441889.1"
                     /locus_tag="Aiant_RS31695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Aiant_63970"
                     /product="DUF6069 family protein"
                     /protein_id="WP_189333697.1"
                     /transl_table=11
                     /translation="MTVEPVKPTVNAGKLWAGGGATAAVAALIAIAGILLGRGIFDVDV
                     LAPKGQGTWGDASTGWYALGAAGAALVATGLVHVLILTTPRPMRFFGWVVSLATVSAVL
                     APFVTDASRASQFYTAGLNMILGIAIGSLVAGVARSATRLVRTDIPPAPRPYPQQYR"
     misc_feature    complement(19434..19448)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(19435..19450)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.97"
                     /tool="antismash"
     misc_feature    19438..19451
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.89"
                     /tool="antismash"
     misc_feature    complement(19438..19453)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.49"
                     /tool="antismash"
     misc_feature    19687..19698
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(20424..20438)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.34"
                     /tool="antismash"
     misc_feature    complement(20425..20439)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.54"
                     /tool="antismash"
     misc_feature    20483..20493
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
ORIGIN
        1 cggtggaccg gaacggccgg ctcttcccgg tcgagctcac ccttcaggcc accaacatgg
       61 gtggcgggcg gccgatgttc cacgcgttcc tgcacgacat cagcgaccgg cgggccgccg
      121 aggagcagct gcgccgacag gccgagctga tcgacgcggc gccggccgcg atcatcgtgc
      181 gcgacccgga cgggaccatc cggttctgga accgcggcgc cgagcaggtc tacggctggc
      241 cggcctcggc cgcggtcggc cgcaacatcc accggctgct ctccacggtc tttcccacgc
      301 gactcgacgc gatcgcccgc gccctggagg agaccgggtc ctgggagggc gaactggtgc
      361 accggcgggc cgacggccgg gtggtggtcg cgctcagccg gcacgtgcgg cggccggccg
      421 cggacggcgc cggtcacgag atcatcgaga cgaacaccga catcaccgag cgccggcggg
      481 ccgaggaggc ccgggaggag agcgagcggc ggttccgggt gcagttccgc cagtccacga
      541 tcgggcaggt gatgctcggg ctggacggcg tcgtcctgca cgtcaacgat gccctggcgg
      601 ggatggtcgg gcggtccgcc gccgacctgt gcgggcacgg cgtggacctg ctgacccacc
      661 cggacgaccg ggacggtgac accgaggcgc tcgccgggct gttcgccgag gagagcgact
      721 cgtacgagcg ggccaagcgc ctgctgcacg ccgacggcca ctacgtcgac gtgcagaccg
      781 gggtgcgcct ggtccgggac gcggacggcc gcccgctgca cctgatcggc gtggtgcagg
      841 acgtcaccga gcagaaccgg gcccgccgcg aacgcgacgc cgcgcaactc gtccttgccg
      901 agcgcaacga gcagctgcag caggccaacc agctgaaact cgacctgatg ggcatgctgt
      961 cgcacgacat cggcacgccg ctcacggcga tcatgggtta cggcgaggtg ctcaccgacg
     1021 cggagctgcc cgggccgctc ggcggggcgg cggcgcggat catcaccggg gcgcaccgga
     1081 tcgacgagct gcggcacaac gtgctggcca tgtgcaagct ggacgccggc gagctgacca
     1141 cggtccggca gccggtcgcg ctgcgtgcgg cgctgcggga cgcggccgac gccgccgacg
     1201 ccgtggtgcc gatcgagtgc ccggacgacc tcgaggtgct cgccaacccc gcccacctgc
     1261 ggcagatcat cgtgaacttc ctgacgaacg cgcggaagta cgggggcggc gcgacccgcg
     1321 tgtccgtcca ctcccgggac accggcgtgg agatcgcggt gcacgacgag ggtgacggcg
     1381 tccccgagtc cctgcgcgag cacctcttcg agcggtacac ccgggccgcc gacgccgcgg
     1441 ccgagggtca cggcctgggc ctgcacatcg tcgccagcct ggcccaggcg aacggcggca
     1501 gcgtcgccca ccgcccgggc gtcccgaccg gcagcgtctt cagcctgcgt ctggaaatcg
     1561 ggtgacgccc ccgccgggat gtgcgagggt ccgacctcat ggaaccgatc agcgaggcga
     1621 cgatccgagc ctcgttcatc aacggcagca aaggcgaggc ggcccggatc aagctgcccg
     1681 ccgacttccg cgacaccccg tggtctgacc tcgacttcta cggctgggtg gaccccgcgg
     1741 ccccgcaacg ggcggccctg ctgctgcccc gcgaggaccg cccggtcgcc gtcctgctgc
     1801 gcaaggcgga acgcgccggc gccggcagcg ccgcccgctc cagcatgtgc caggtctgcc
     1861 tgaccgacca cgcggccggc ggcgtcagcc tgttcaccgc cccgctggcc ggtgccgccg
     1921 gccgcaacgg caactcggtc ggcgagtacc tctgcgccga cctcgcctgc tccctctacc
     1981 tgcgcggcaa gaaacgcccg agactgcgcc tggtccgctt cgaggagacg ctcaccctcg
     2041 ccgagaagat cgaccgagcg ctaaccaagc tgtccgcctt caccgcacgc atcgctgccg
     2101 cataaaccca ttttgtacga tctatccaca gtcccgtggc tacccgcacg gcccgccgtc
     2161 cgtccggacc gcgaggccgc gccgggctga tgcggcccct gacggtccga ccacccgcga
     2221 ggccgcgccg ggctgatgcg gcccctgacg gtccgaccgc cccgccgccc gcaacggtgc
     2281 cgccgccacc ggcgcgggtc gtgtgcccgc cggacctccc gtggtcagtg cgggccggcg
     2341 ggcgcggttc gttgcgccgg cgattgccat ccgcgcggac ttccggacga tcgttcaggg
     2401 cgcatgcctc cgatctcccg gaacggcctc gtcgtccctg atcgtctcga ggtgaagatc
     2461 cgcttgtcga gccaccccca tatgcgcggg ttcgcccgat cggcgatgag cagaaaaccg
     2521 agtatgccgc taccggcgag catgcccccc agtgaatcgc tgagccagtg gtgacctgac
     2581 acgacattcg ccaccgcggt cacggacacg gccgaggcca ggattgttct gcgggcttga
     2641 gagcttgccc aatgattgag caagatcagg atgaggccgt accagacaac agagttcgcc
     2701 acgtgcccgg agggaaacga ttggcaagca ggccctccgc acggctcctt gctgaagaat
     2761 tcgaccgcgc ccggctgggg gtcggccggc gctccgcgac gcgtatacat ctttacggac
     2821 ccgacaatca tgatcagtga tgccgacgtg catgcccaca gcgcaaaatg cttcaccgtc
     2881 ttcaatcgga aagactgtgc agcggcgatg acaagagaaa ggtacatcag cggaccgccc
     2941 tgcccgaggc gagccgctgc cttgctcgcg gaaatcaacc agtgtggcag gtcgcgcgct
     3001 atcccatcgc gcaccgcgat gtctgtatcg atgaagaagt ggacggctat gagcgcggtg
     3061 agcagtgcgt acagcccgaa tgccgcgccc atggacgcca ggtaaaacct atacttcttc
     3121 acggcgcgcg atcatacacg aacggagcga cgcaaaagcg gctagtcgat tattgtttcg
     3181 gcgtggggga tggattcatt tgttggcggg gcttggcgaa tgatgtgatg gacggcgagt
     3241 cgtgtcgacc gtggtattca gaccaggcag tcggtcgttt gctccatggt gatcgtggca
     3301 acgcgacagc ctttcgtgca cccaggaaca gccatggaac gggccgctca tcgccgaccg
     3361 cgcgatccgg acgcggtcag tgggagtcgg ccgggttggt gtgccgggcg gcctggcgga
     3421 ggtcgatgtc ctcgatcagt tcgcgggcca gttcctcggt gatccggcgt tccatcacgg
     3481 cccggccgag ggccaggcgc tgcgcctcga acgaggcgtc cacgtccggg gccggcgcgg
     3541 cggccgcctc ggcgacgatg cggtggccct cggtgcgcag ctcctccgcg gcggcgtgtt
     3601 cggcggtcag gtcgaaccgg aggcggcggg ccagccagcc gaccgtggtg ccctggagca
     3661 gcagcgtgcc gagggtgacg atgagggcga tggcctggat cgcgtcgcgg ccggggaacg
     3721 gcgcaccgga cgcggtgtgt tccgggatcg cggcggccgc ggcgagggtg aggatgccgc
     3781 gcatgccggt ccagccgacc agggcgttct ccttcatcgt cgggggcggc agcggctcgc
     3841 cgccgcggcg gcgagcgacc cgtcccgcgt ggaccctggc ggctcgcggg tcccgggcga
     3901 tccggcggtc gatcatgcgc tgcttcaggg tccagcggcc gaacagcccg aacacgccga
     3961 tgaggcgcgc cgcgatcgcg gccgccagca gcacgccggc ggcgatcagg gtgcgggtca
     4021 ggcccggctc ggccgagtcg gccaggtcgt ccaggacgaa cctgagctgg aggccgatgt
     4081 aggcgaagac gaacgtctcc agcaggaagt cggcgacccg ccacacctgc tcctcctgga
     4141 gccgggtgcg gtagccgccc gggtactggt gtctcgggtc gagggtgagg ttggcgctca
     4201 tcaggaacgc ggccgcgacc accgcgagga tgccggaggc gtgcacctcc tcggcggtga
     4261 ggaacgcggt gaacggcagc agcagcgaca gcgacgtctc cagcgtcggg ttgccgagtc
     4321 gtttgcgcac ggtgaggccg agcaccgcga aggccccgcc gatcagcacg ccgagcgtgg
     4381 cggtgcgcag gaactcgccg atgccgccgg tccaggtggc gtgctcgccg ttgaccgcgg
     4441 cgaccgcgat gctgaacagg gtcagcgcgg tggcgtcgtt gaccaggctc tccccggtga
     4501 ggatcgaggt gacccgtttc ggcaggccga tctcgtcgcc gtgggagacc gtcgtgatgg
     4561 tgtccggcgg ggccagcacc gagccgagca cgaacgcggc ggccaccccg atcgacggca
     4621 gcagcagcga ggagagccag ccgagcaccc cggcggtgag caccacgagg accacgccga
     4681 gcgagacgat ggggcgcagg ttggcgccga agccggagac cgaggcgccc cgggtcgcgg
     4741 accagagcag cggcgggatg acgatcgcca ggatcaggtg gctctccagc tccagccgcg
     4801 gcaccccggg gatgaaggag gccgcggcgg ccagcaccac gatcacccag cccggctcga
     4861 accggtgccg gtgggcgacc gacgtgaccg cgatcccgac cccgatgatg agcagcagct
     4921 gcaccccgtt cacgagatca atctaggcct gctcagggca cggcgcccag catggtgagc
     4981 acgtcggtga acatggtgac ggtggccacc gggtcgaggt gctcggcctg ccagccacgg
     5041 gtgcgcacgc cctgcaggac accgatcatg gtggtccgca cggtgcgcag ggtggcgggg
     5101 gagagcagca cggccgaggt gccgtcggcg tgcgcggccc gggaggcggc gccgagcgcg
     5161 accagcacgg ccgccgaccc gttgaggatc tgcgccacgt gcaggcggtc ccggtccagg
     5221 ttgaaggtgg cgacgtgcac ggccagcccg agcccggcca gcagccgccc ggtctcgtca
     5281 ccgctgagca ccgccggcat ggtcgctgag tcgtcctcgg ccaccgccgc cccgtccccg
     5341 aacccgctca ccagctcggc cgccacctcg gccggcaccc acaactcggc cggcgggacg
     5401 tcggggaaac ggcggcgcag cgcggcctcg accgcgtccc ggatcacgta accggggacc
     5461 accttgtgct gctcggcgag ccgtcccatg atcaccgcga ccacgtcgtc gggggtcatc
     5521 ggccgctcca tccacctacc gtatccgggc tcaggccacg gcgggcaggg tcacccgggc
     5581 ggtggtaccg gccgggccgc tggtcacagt gaggtccccg aggtgtgcgg cgatcgaggc
     5641 gcgggcgatc gtcagtccca ggccgacgcc ggggatggcc tgctcacggg cgtgccgccc
     5701 gcgatagaag cggtcgaaga cgaaggccag ctcgtcgtcc gggatgccca ccccgcagtc
     5761 gatgacctcc accgtgggcc ggggctcggc gccgacccgg atcgtgatcg gccggtcggc
     5821 cggggtgaac agcagcgcgt tgcgcagcac ctgctcgatc gcgtgggaca gccgggccag
     5881 gtcggcctgc acgacgatcg gcgggccgga gaacacgtcg accggcgcgg cgcgcagcgc
     5941 cgtgagcgcc cggcacgagg tcagcgccgc ggcgaccacg gtgttcaggt cgaccgggcc
     6001 gaccggggcg ggcagcggcg cgtcgtgcgg gcgggtgccg gacaggatgt ggtcgaccat
     6061 cgcgaccagt cgctgcccgt tgcggtggat cggctcgatc aaccgcttgt acgccgccag
     6121 ctcctcgttc tcgctgagca gctccaggta tccctggatg ctggtcaccg gggtacgcag
     6181 ctcgtgcgtg atggtggcga ccaggtcgtc ctcgcgctgc gcggcccggg acaggtgatc
     6241 gccgaggtcg gccatccgca gccggttgcg gatcgcggac aggtgcgtgg cggcctgccc
     6301 ggcgaacgtg gtgagcgcct ccacctggcg gtcgctgacc tcgcggggca cgtcgtcgat
     6361 cacgcacatc gcgccgagca cgtgcccgtc ctcgtcgatc accggggcgc cggcgtagaa
     6421 ccggatgttc ggtgcctcga ccacgttgcg gtacccggag aacagcgggt gcgcggtggc
     6481 gtccgggacg atcagcgggc ggcgggccgg caggatctgc gcgcagaacg acacgtcgag
     6541 cggggtctgc gcgtcggcca ggccgatgct gccggcgaac cactgccggt cccggtcgat
     6601 cagcgacacg gccgccatcg gggtgtcgaa catggtggcc gcgagccgcg tgagatcgtc
     6661 cagcaccgcc gcgcggggct cgtccagcac ccggtaggag tgcagtgcgg cgagccgggc
     6721 ttcgtcgtgg acctcgtcgc tgacctcgtc gctcatagcc tgaccatcgg cgcggcatcg
     6781 gcgatcctga gggtctgtga tccgttaccc ggtgaaccag agccgcatcc ggtcggtgtg
     6841 tcccgaccag gtcagggtga ggtcccagca ccccggctcg gggatcttca cggccgacgg
     6901 cccggcgccg ttcggcagct cctgggtggc ggtgagaccg tccgagcgga cgccgctgac
     6961 ggtcagcggg gcggccgtct cgaccggcag acgggacacc cagaggatct tggtgccgtc
     7021 caccgggtcg gctttcggcg gggcgaacag gatcgccatc aggccgccct ggtcaccgag
     7081 gcggtgcggg acgccggagc cgtcgaagtt gaagccctcc cgggcccaga ccgggagggc
     7141 ctcggtgctg atcccggcgt tgcactcggc cgacgacacc gccgccccgg taggagccgg
     7201 agcggaggtg gcgggaccgt aggtggcgct cccggacggt gggtccgagc aggcgccggc
     7261 gaacatcagg atcgtgacgg tcgtgcaggc gacgggcgta cgcaaagcaa ccacctcctg
     7321 cctggcactg tggcagccac cgcgaccggc ggaccacccc cggcgccacg acccgcacca
     7381 tctgcccacc ggcgggaaat cgggaaccga acgggtcaga gcttgcgggc gacggtgtgc
     7441 ttgccggaga gcttgagccg gtaggcgatc cggacgccgg tgtaggggtt ggtggcggag
     7501 atcgtgacgc cgccggtgac gttccggacc gtgaccacct tgacggcgag gctctcgttg
     7561 aagccggcga cggcggcgcg cgcgcccttg cgcacggcgg ccacggtgac gttccggccc
     7621 cgggcccggt cgtcgacccc gatcatgatg tccgaggtca gccgggacat cttccgggcc
     7681 aggtcgtagt aggccaccgc cttggtcagc gtcgtctggc tgatcacctg gccggtggcg
     7741 ggcagcgtga cggtctgcgg tccgtagccg aaggtgatcg tctgctcgac cccggtgtcg
     7801 accacccggc cgcgggtgac caccccggtg gcgtcgacgg tgaaggtgac ccgcgtcttc
     7861 tcctcgccgg tgaaggagta gtcggcggag ccgtcgtcgt gggtggtctt ggtgccggcg
     7921 tgccgggcgt cctcgacgac gatctccgag ggcgcgctca tctgccggct gctccagccg
     7981 tccagggtga gcttggcgtt cggcacgaag acgtagcggg cctcggggcg gccggccatc
     8041 ttcgccgcgg cgcgctcggc cgagccggtc aggtagtcgt actcgccctt gccggcgacc
     8101 gcgtagacgg cctcggtgcc gtacccggtg taggccatcc ggtagtagcc gcggccggtc
     8161 gccgggtcga cggcgaacgc catcgcggcc accctcccgt ccagggtcgc ccgcagcgag
     8221 ccggcgtagc cggggatctc cgccgtggcg gtcgtcgcgg ccaccgcctt gagcgccgcg
     8281 gacatctcgg ccgcggtcag cgacgtgctg gtgtcggccg cgagggcggc gccgggggcg
     8341 gccagcacgc cggcggtgag cgaggcggtg gtgaggagca gggcacggaa caccggaggt
     8401 gtcctttccg gaacaggagt gagtcgccgg ttccttcggc accccggtcg gccacttgag
     8461 aaccgccctt cgcgacgaaa ttgctggtca gagcatgtgt gcagattccg cgaatcccga
     8521 caaacaagtc agatatgcga tagaacgacc ataagtaggc acggaccgaa cgacgaacgg
     8581 ggttacgagc ggtggccact cccacgcagt ccgagattcg ggacgacgag caggagcaac
     8641 ggcggcagag cctgagcacg gcggcggccc ggaacctgac gaccaccacc aagacggttc
     8701 cgcagatgca ggagatcagc tcgcgctggc tgctgcgcaa gctgccgtgg gtgcaggtgt
     8761 ccggcggcgc cttccgggtg aaccggcgga tgacctaccg gatcggcgac ggccggctca
     8821 gcttcaccaa cgtcggcgcc caggtccggg tgatccccgg cgagctgcgc gagctctccg
     8881 tgctcagcga gttcgacgag gccgaggtgc tggccgcgat ggccgacaag ttcgtgcagc
     8941 aggagtacca gccgggtcag gtgatcgtcg agttcggctc ggtcgccgac cacgtctacc
     9001 tgatcgcgca cggcaaggtg aacaaggtcg gcgtcggcaa ctacggcgac ccggtcaacc
     9061 tcggcgtgct ggccgacggc gaggcgttcg gcgagtactc gctcaccgac gatcagcgga
     9121 tctgggacta caccgccaag gcgatgacgg cggtgaccct gctggccatg ccgcggtcgg
     9181 cgttcaccgc cctgctcggg cagagcgacc ggttgcgggc gcacgtcgac cggttccggg
     9241 ccaagaaccg ccggccgcag aacaagcacg gcgaggcggc gatctcggtc gcggccgggc
     9301 acaccggcga gccgcagctg gacggcacgt acgtggacta cgagcagagc ccgcgggagt
     9361 acgagctgag cgtcgcgcag accatcctgc gggtgcacac ccgggtcgcc gacctctaca
     9421 acgatccgat gaaccagctg gagcagcagc tccggctgac cgtcgaggcg ctgcgcgagc
     9481 gccaggagta cgagatcgtc aacaaccgcg agttcggcct gctgcacaac gccgacctgc
     9541 ggcagcgcat tcacacccgg agcgggccgc ccaccccgga cgacctggac gagctgctga
     9601 gcatgcggcg ggccaccaag atgttcgtgg cgcacccgca ggcgatcgcg gcgttcggcc
     9661 gggagtgcac gaagcgcggg atctacccgc cgatggtgga gcacgacggc ggcacgttcc
     9721 tgtcctggcg cggggtgccg atcctgccgt gcggcaagat cccggtgacc gagacgcaca
     9781 ccacctcgat cctggcgatg cggaccgggg aggcggacca gggcgtggtc ggactgcacc
     9841 agaccgggat cccggacgag taccagccga gcctgtcggt gcggttcatg gggatcgacg
     9901 agcaggcgat catgtcctac ctggtcagcg cctactactc ggcggcggtg ctggtgccgg
     9961 acgcgctggg catcctggac cacgtcgagc tgtcgcactg atggtgctca ccctcgacct
    10021 cggcagggag atcctgcgcg acctgcgtgc ggcgccgtcc ggtgatctcg cggcggtgct
    10081 gggcgccgta ccgggaaccg gcgggccgat gacgagcggt ccggcgccgg acggcgtacc
    10141 ggcaatgatc ggtcttgggc cgagcggact cggcacctcc gccgcgcgtg cctttcgtcc
    10201 cgtgccggcg cggccggacg gcccgcatct gcgctgtccc gggccgctgc gggacgaccc
    10261 ggcgctgggg gagcgggtca acgaggccgt cgtgcgctgg gccgagggga tcggcatcta
    10321 ccccggccgg ttggacacgc tgcgctcggc gaacttcggc cgcttcatga tgctggccca
    10381 cccggccacc agcgacccgg accggctgct cgccgcgacg aaatgcctgg tcgcggagtg
    10441 ggcggcggac gactactacg tggacgaggt gtcgctgggc gccgacccga tggtggtcgg
    10501 atcgcggctg gccaacctgc acgcggtggt cgatccggtg tcgatggtgc cgcggtacct
    10561 ggccgattac cgggaccacc atcggctgct gccgatctcg cgggcgttcc gcagcgccat
    10621 ggagcacttc gccgagtacg cgagcgtggc gcagctgggc cggttccagc accagatggc
    10681 catcctgttc ctggcctgga cgcaggaggc ggactggcac gcgaaccggc gcacgccacc
    10741 ggtctgggag tacctggtcc agcggcatct caacagctac ctgccgccga tgatcctgat
    10801 cgacgtgctg ggcggttacg agcttccggc cgacgagttc taccacccgc gggtgcggcg
    10861 ggcgttcacc acggcggcca acgcggccgt gctcatcaac gacctgtact ccgggaagaa
    10921 cgagtccgag aacgatcaca acctgccgac cgtgctggcg gcgaacgagg ggctcggtca
    10981 ccgggcggcg gtgctgcgga ccgtcgagat ccacaacgag ttgatggacg acttcgtccg
    11041 gctggccggg tcgctcagcg tgaccgggtc gccgcagttg cgccggttcc tggcggacac
    11101 ctgggcgtgg ctgggcggaa gccgggagtg gcacgccacg agcggccgct atcacgactc
    11161 tcaggaggag aaatcatgac aacgacggcc gtctcgccgc tgcggaccga cttcgagcgc
    11221 tcggtcgcga actactggaa caccaaccgg gtcgacccgg tcaacctgcg cctcggcgag
    11281 gtcgacgggc tgtaccacca tcactacggc gtcggcgagc cggactggag cgtgctcgac
    11341 ggtcccgagc cgggcgtgat cgccgagttg caccggctgg agagcgctca ggccgacctg
    11401 ctgctcgacc atctcgggcc gatccggccg gaggacgcgc tgctcgacgg cgggtcgggt
    11461 cggggcggga ccagcatcat ggccaacgcc cggttcggct gccgggtgga cggggtgtcg
    11521 atctccgagt accaggtcgg gttcgccaac gagcaggccg cccggcgcgg ggtcgccgac
    11581 cgggtccggt tccacttccg gaacatgctg gactccgggt tcgccgacgg gtcccggcag
    11641 gcgatctgga cgaacgagac gacgatgtac gtcgacctgt tcgacctcta cgcggagttc
    11701 gcccggatgc tccggttcgg cgggcgctac gtctgcatca ccggctgcgc gaacgacgtg
    11761 accggccggc ggtccaagtc ggtcagcaag atcaacgagc actacacctg cgacatccac
    11821 ccgcgcagcg actacttcaa ggcgctcgcg gcgaacgacc tggtgccgat caacgtggtc
    11881 gacctgaccg ccgcgaccat cccgtactgg gagctccggg ccaagtcgga ggtggcgacc
    11941 gggatcgagg aggcttttct cacggcgtac cgggaaggca gttttcacta cctcctgatc
    12001 gccgccgacc gggtctgacc agcccggccg gatgcgccgc cgccgggggc ctcgtgcccc
    12061 cggcggcggt ttgttttccg gcgccggggg cactgatcga gcatggcgct gccgatcgag
    12121 gactacgcga tcatcgctga cacccagacc gccgcgctgg tcggccgcaa cgggtcgatc
    12181 gactggctgt gcgtgccccg gttcgactcc ggggcgatct tcgcggcgct gctcggctcg
    12241 gccgacaacg gtcactggac ggtcgctccc acggacgtga tcaccacccg tcgccggtat
    12301 cgcgacgaga ctctggtgct ggagaccgag ttcgagaccg ccggcggcgt ggcccggctc
    12361 atcgacttca tgcccccgcg gaccgactcc ccatcggtga tccggatcgt cgagggtgtc
    12421 cgggggcagg tcgacttcag catggagctg cggctgcgct tcgattacgg gcacgtcgtc
    12481 ccatgggtgt accgcgaggg cgacgcgctg gtcggggtgg ccggtccgga cgcggcgtgg
    12541 ctgcggaccc ccgtcgatct gcgcggcgaa aacctcacca caaaagccga tttttcagta
    12601 cgagctggtg agcgcgtgcc gttcgtgctc acctggcgcc cctcccacct gccaccaccc
    12661 gagccgctcg acccggccca cgaactcggc gtcaccgagg gttactggcg cggctggatc
    12721 tccgcctgca cctacgaggg cgagtggcgc gacgccgtgg tgcgctcgct gctcaccctg
    12781 aaggcgctca cctacgcccc gaccggcggc atcgtcgcgg ccgccaccac cagcctgccg
    12841 gagaagctcg gcggcgtgcg caactgggac taccggttct gctggctgcg cgacgccacc
    12901 atcacccttc aggcgctgct cttctccggc ttccagagcg aggcgaccgc ctggcgcaag
    12961 tggctgctgc gcgccatcgc cggcaacccc gccgagctcc agatcatgta cggcgtcggc
    13021 ggggagcgcc gcctcgacga gtacctcgcc gactggctcg gcgggtacga cggcaacccg
    13081 gtgcggatcg gcaacgcggc ggccgaacag ttccagctcg acgtgtacgg cgaggtgatg
    13141 gacgcgctgc accagggccg gcgggccggg ctcaaggcgg acgacccggc gtgggggttg
    13201 caggtcaaac tgatggagtt cgtcgagaac cactggcagg acccggacga gggcatctgg
    13261 gaggtgcgcg gcggcccgaa gcagttcacc cactccaagc tgatggcgtg ggtcgcggcc
    13321 gaccgggcgg tcaaggcggt cgaggacttc ggcctggacg ggccgctgga gcggtggacc
    13381 gagctgcgct cccggatccg ggaggacatc ctggagcacg gttacgaccc ggatcgcaag
    13441 acgttcaccc agtactacgg ctcggcggaa ctggacgccg cgatgctgat ggtcccgctg
    13501 gtcggcttcc tgcccgccga cgacgagcgg gtggccggca ccgtcgcggc gatcgagaag
    13561 cacctgctcg tcgacggttt cgtgcagcgt tacacgcagc acccggacac cgacgtggac
    13621 gggctgccgc cgggggaggg ggcgttcctg gcctgcacgt tctggctcgc cgacaattac
    13681 gcgctgatgg gccggcacga cgaggcccgg gagacgttcg cccggctgct ggccttgcgg
    13741 aacgacgtgg gcctgctgtc ggaggagtac gacaccgtgg ccggtcggct ggtcgggaat
    13801 tttcctcagg ccttcagcca tgttccgctg attgatacgg ctcggacgct gagtagtgct
    13861 ttgggtccga ctgaggctcg ggtgcgggag gggctcaagt agcgcctggg cggggaggtc
    13921 gcttaccgct cttgccttct gccttcggtg cggtggcccg cgctattgcg gaacattgct
    13981 ggaaaaaata ttgggttggc acgctcgggc actgaccggt gaggtgtcgg gggcggaaca
    14041 attgattcat gcggcagttg tcgttcttcg ggaaggcgga tctcgcgaag atgcgggacc
    14101 ggactgctgc gcgcaattat tcaccggaga aggacgaatt ccgccgtgag caggagcgac
    14161 gccggaaatt cggtctgcgt cgccgacatg ctgagaaact gcggcgcctc ggccacccgg
    14221 acgccgaagc catagccgcc accatgcacc gggtccaccc tccgggccga ccaccccagc
    14281 cccagtcggc gccagcccgg ccgcggccaa gtcagcgtca gccgcagccg atccagccgc
    14341 aaccgatccg gtcgcacccg cagcaggcca ggccgcagcc gcagccaatc ccgtcaccgc
    14401 cgacccggcc gcagccgcag ccgcagccga tccagccgca gccgcggcag gccaggccgc
    14461 agccgcagcc aatcccgtcg ccgccgatcc agccgcagcc gcaaccgcaa ccgatccagc
    14521 cgcagccgcg gcaggccagg ccgcagccgc agccaatccc gtcgccgccg acccggccgc
    14581 agcggcagtc gacccggccg cagccgcagt cgatcccgtc gcagccgcag tcgagccggc
    14641 cccggccggt ccaagcgcag ccgcttcagg cgcggcccat cgtcgagccg ctgcgggttc
    14701 ccgggagttg tccgacgaaa gccgcgctca ttgagcagaa accgcaggcc cgcgctcctg
    14761 ccacgcggtc ggcgagacag ccgactgcat caccggccag gcggtccgtg ccgcgaacgt
    14821 cgatgtcgcc gaagccgcga ccagcggcat tgccggcggt gctgcacgct ctgcaactgg
    14881 cgtcgccacc gtcactggcg accaccaatg ggtccacacc gttgcgggtg gggcacgttt
    14941 cgcgatcggc cgcagcgctg gtgatgcggc acgtttcccg accggaggca ttgtcggctg
    15001 cgctgcacgc tctgcagccg gcgtcgttgc tgacggtgcg gcatgtttcc cggccggcgt
    15061 catcgctgct ggtgtggcac gtttcgcgat cggccgcagc gctggtgatg cggcatgttt
    15121 cgcggccggc gtcatggctg gtggtgcggc acgtctcgcg accggccgca gcgctggcgt
    15181 tgttcgtggg cgcgccggtt gtctcggccg tatcgggatt ctcggtgcct gcgccatctg
    15241 gggtgctgac tgtccgggcc gctttgttct ccttgtcggc cgggaggacg cgcgctttcc
    15301 ggtggcgggt ggtgacaagt ctatcggtgc gggtgcgtgc ggggcgaggt gggaatgaaa
    15361 tgaatgaggg tggtcactgt ggggcggtga taaaggtgac actcggggtt gtccgcgaca
    15421 atcgggttcg tgggtctccc tgtcgggtgg ctcgggtttg agagatgatt cgcgcttatt
    15481 ctttttgtat tctggctccc gggtggtgat tcgtggcggt atgacttttg ggggaattta
    15541 cggatctttt cagggcgcag ttggagcgcg ggtgacctgt ggatcgtccg ctgtccctcg
    15601 tggtgggtga cggcatgcgg cgcgtcgggc ggtgcggcgt ggtggtctcc gacgtctggg
    15661 tcggtgcagt tgcggagccg gcggggattc cgggcgtcgc ctcgtggggc gaacgataac
    15721 cgacgggtta atcggcggtt cgatcttttg cgttgttcgg tggggtcggt ggggcgatcg
    15781 ttggtggcgt gagagtgacc atgatcgaca ttggggtgtg ggcgacgcgg gcgatggcgg
    15841 gggtggccgg gctggggccc atcattccgc tggggccccc ggagtgcggt cggggcggtg
    15901 gcccgcggga ccgaaggagg ggcgtcggat cgcgggtacg gctgtggagc ctcgggacca
    15961 cagcgtggtt tcggggttgc cgggggcggc cggttccgta gcgtgtgggg gcatgcggga
    16021 aagaaaactg ggggatctga cggtctccgc gatcgggttc ggcgcgatgg ggatgagcca
    16081 cggttacggg cccgggccgg atcgggacgc gaacatcggg ctgttgcggg ccgccgtcga
    16141 gcggggggtc acgcttttcg acacggccga ggtctatggg ccgtgggtca acgaggaact
    16201 ggtcggtgag gcgctcgaac cgttcaaggg gcaggtggtc atcgccacca agttcgggtt
    16261 cgtcatcgat gacgacgggc ggcagggcgc cggggtgaac agccggccgg agaacatccg
    16321 gaaggtcgcg gaggcgtcgc tgcggcggct gcgggtggac gcgatcgacc tgttctacca
    16381 gcaccgggtg gatccggacg tgccgatcga ggacgtggcc ggaacggtgc gggacctgat
    16441 cgcggctggc aaggtgcggc atttcgggat gtccgaggcg gcccccggga cgatccggcg
    16501 ggcgcacgcg gtgcagccgg tgaccgcggt gcagagcgag tattcgctgt ggtggcgccg
    16561 gcccgaggag gaactgctcg acacgctcgc ggagttgggc atcggcttcg tgccgttcag
    16621 tccgctgggc cgcggtttcc tgaccgggaa gatcggtgcg gacaccgttt tcgccgagaa
    16681 tgacatgcgt aacgggctgc cccggttctc ggcggcggcg cgggaggcga atcaggcgct
    16741 ggtcgagctg atcgggcgga tcgcggccgg caaggcggcg accccggcgc aggtggcgct
    16801 cgcctggctg ctggcgcagc ggccgtggat cgtgccgatc ccgggcaccc ggcggctgga
    16861 ccggctggac gagaacctgg gcgcggccgg gatcgaactc accgcggccg acctcgcgga
    16921 gatcgagacg gccgccgcga agattgaggt gcaggggagc cgctaccccg agcagatgga
    16981 gaagatgacc aacctttagc cgacggcggc gcggcgcggc gcgtactcct tctcctcgta
    17041 ctgctcggtc cagcggcggc tgctcgactc cagcgggtcg gtgtcgtaga ccgaccggcc
    17101 gtggaaacgc tgcgagtagt cgatcatgcc gtcgacgagg cggtccacgt caccggcggg
    17161 ccggaccacg aggcgcatga actggctggt catgccgagc ttgttgccgc actcgcgggt
    17221 catgatgccg tggttggcga ccaggaagtc gcgcagcgcc acgccggacc actcggtcgg
    17281 cagcttgacc aggaagaagt tgccctgcga cgggaagacg gtgaggcccg ggatgcgctg
    17341 gagctcccgg cccatcgacc ggcggtcgcg gctgagcagg cgcaggctct cctcgtactc
    17401 catctcgtgc tgctcgatca tgtggaccac cttctccgcg agggagttca ggttccactt
    17461 cggcagcatc ttcgcgatct tgccggcgat cgccgggttg gccagcatgt agccgaacct
    17521 gatgccgtgc agaccgaagt tcttgccgag cgacttgagc accaccgtgt tcggccggat
    17581 caccgcgtcc gggccgaccg aggggtagcg ctcggcgtcg gagaagtcga tgaacgactc
    17641 gtcgatgacc accaggtcca ggtcgctgag ctggtccatg aaccggatga cgtcccgccg
    17701 cggcaggtag ttgccgtccg ggttgttcac gttgcagacc accgcgaccc gggaccggcg
    17761 ctcccggatg aactcgacgt actcctccag gtcgagccgg aagccgtcgc gttcctggag
    17821 cgggaacatg tccacccgct tgccggtctc cagaggctgg tcggtccacc ggccgaaggt
    17881 cgggatcggg atggccaggc tctccttgac cagcaggtgg tcgatccagg tgatcagctc
    17941 ggtggagccg ttcgccatcg ccaccgtctg cgggtgcagg ttgagcacgg cggccagctt
    18001 cttggtgatg gtggccgagt cgctcgggta gtacttcagg atgctcttca gctcccgggt
    18061 gagctcgtcg aacatctccg gggtggggaa gtacgggttg cacgggatgc agaagtcgac
    18121 gatctccgcg ccctcgccgg ccgctcgggc caggtcgaag taggaggcgc tgtgagctcc
    18181 cttgtgcagg atcgcagtgt cgattccggt ccgtgccacg gtgtcctccg gatgcctctc
    18241 ggtgacgtta ccgcccccga gtacgggact cgcagcccga tcgttcacac catcgttgat
    18301 ccacacggag gtttaagccg gaggaaagga acacaatgcg ggggatcgtg cgtaccggat
    18361 tgatcggatg tgtggtgctc ggatcggtga ccgtggcgga cgaggcgcgc gcccggccgg
    18421 gtggcgggga gccggtccgg gccgtgcagt ggacggtgga cagtggcgcg cggcggcatc
    18481 cggtaccggt gccgggtgac gtccgcaagg gctgctgacg ctttcacccc cgtcggatga
    18541 ggcggtcatg cccggcccac ggcgatcctc agcgccatga ccgtcgaacc tgtcaaaccc
    18601 actgtcaacg ccgggaagct gtgggccggc gggggcgcca ccgcggccgt ggccgcactg
    18661 atcgccattg ccgggattct gctcggacgc ggaatcttcg acgtcgacgt cctggcgccg
    18721 aaaggtcagg gcacctgggg agacgccagc accgggtggt acgccctggg tgccgccggc
    18781 gccgcgctgg tcgccaccgg cctggtgcac gtgctgatcc tgaccacccc gcggccgatg
    18841 cggttcttcg gctgggtggt cagcctggcc acggtgtccg cggtgctcgc gccgttcgtc
    18901 accgacgcga gccgggcgtc gcagttctac accgcggggc tcaacatgat actcggtatc
    18961 gcgatcggtt ccctggtcgc cggcgtcgcc cggtccgcga cccggctggt ccggaccgac
    19021 atcccgcccg ctccgcggcc ctatccgcag cagtaccggt agaggggggc ctgagatggc
    19081 gaccatgaca gaaccgccgg agacgccggt ctttcacgtg ctcaccgtgc cggaggcgct
    19141 ccaggccgag aacgtcgacc agcagcgcgg gctgagcagc gacgaggcgg cccagcgccg
    19201 ggagagatac ggcccgaaca agttcgccga ggccaagaag gaaccctggt ggcgctcctt
    19261 cgtccggcag tacgccgatc cgatgcagat cgtgctgctg gccgccggca tcggcagcct
    19321 ctacccgctc aagcagtggg gcaccggcgt catgctgatc gccctgaccc tgctcaacgc
    19381 cttcctcggt ctgcaccagg agggcaaggc ggcggccgcg atcgacgcgc tccagaagat
    19441 gatgatcatc aagacccggg tacgccgcga cggcaagctc gaggaactgc ccgccgagga
    19501 gctggtcccc ggcgacgtgg tgctgatcga ggccggtgac gtggtcccgg ccgacgggcg
    19561 gctgctgcgc gcggccaccc tggaggtcgc cgagtcggcg ctgaccgggg agagcctgcc
    19621 ggtcgccaag aacgtcgagc cgctggagaa ggtcgacgcc gacctgggcg accgggccga
    19681 catggcgtac atgaacacca acgtgacccg gggttccggc gagctgctgg tcaccgcgac
    19741 cgggatgaac accgaggtcg gccggatctc cgggatgctc cagcaggcga aggacgagga
    19801 cacgccgctc acccggcagc tcgccaagct gaccaaccaa atcctgtaca tcgccgggtt
    19861 cgcgctgctc gcctcggtcg tgatcaacct ggcccggggc aacgagttca acgtggtctt
    19921 cacggccgcg gtcgccttcg cggtctcggc catcccgacc ggcctgccgg ccgtggtcac
    19981 cacgatcctg tcgctcggca cccagctgct cgccaagtcg aacgcgatcg tcaaacggct
    20041 gcggtccacc gagacgctcg gctccacctc ggcgatcaac tcggacaaga ccgggacgct
    20101 gacgctgaac cagatgaccg ccaccgagat ggcgatcccg gggcggcggt acgtgatctc
    20161 cgggagcggt tactccaccg agggcaccat caagcgggtg gcgggcgagc cggacacccc
    20221 gctggaggag tatctgctgc cgatgatcct ggcctccgac gcggtggtca gcgacggcac
    20281 catgatcggt gacccgaccg agggcgcgct ggtggtgctc gccgagaagg gtggtctgga
    20341 cgccgaggcg acccggcaga agtacccgcg ggtggccgag ctgccgttcg acgcggcgta
    20401 caagatgatg gcgacattcc atgaaatgtc gggaaaaatt aggtgcttcg tcaagggcgc
    20461 gccggaccag attctcgctc gctgcaatgt gacggacgag cagcgcgaac gctacctcgc
    20521 cgagaacgag cgcctggcca aacagggcct gcgtgtcctg gccaccgccc gtaaggacct
    20581 cgatgccgtc gacgccagta aagatctcct cgagctgctg accgacctgg aaccgctcgc
    20641 cctggtcggc atcgtcgacc cgccgcggcc gcaggccaag gccgcgatcg agcaggcaca
    20701 cgcggccggc atcgaggtcc gcatgatcac cggtgaccac gcgatcaccg ccgccgcgat
    20761 cgccggcaag ctcggcatcc gtggccgggc gatcaccggc gccgagttcg ccgcgatgag
    20821 cgacgacgag ctggaccggg agatcgacgg catcggcgtg atcgcccggg tcaccccgga
    20881 gcacaaggta cgccttgtcg acgcgctcaa gcgcaagggc cacatcgtcg cgatgaccgg
    20941 tgacggcgtg aacgacgcgc ccgcgctgaa gaaggccgac atcggcatcg cgatggggat
    21001 caccggcacc gaggtgagca aggaagccgc cgcgatgatc ctcaccgacg acgacttcgc
    21061 caccatcgtg aaggccgtcg agctgggccg ggccctgtac gccaacctga agaagtacat
    21121 cttcttccag atgggcgtgc tggccgccat gatcgtcacg ttcctcgggg cgagcatcg
//
