LOCUS       NZ_CP073318            10816 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Kutzneria sp. CA-103260 chromosome, complete genome.
ACCESSION   NZ_CP073318
VERSION     NZ_CP073318.1
KEYWORDS    RefSeq.
SOURCE      Kutzneria sp. CA-103260
  ORGANISM  Kutzneria sp. CA-103260
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kutzneria.
REFERENCE   1  (bases 1 to 11609901)
  AUTHORS   Jorgensen,T.S., Gren,T., Kontou,E.E., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Complete Genome Sequence of the Rare Actinobacterium Kutzneria sp.
            Strain CA-103260
  JOURNAL   Microbiol Resour Announc 10 (30), e0049921 (2021)
   PUBMED   34323613
REFERENCE   2  (bases 1 to 11609901)
  AUTHORS   Joergensen,T.S., Gren,T., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2021) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-APR-2021
            Assembly Method :: Flye v. 2.8-b1674; unicycler_polish v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:18:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,326
            Genes (coding)                    :: 10,257
            CDSs (with protein)               :: 10,257
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 9 of 69
            Pseudo Genes (incomplete)         :: 63 of 69
            Pseudo Genes (internal stop)      :: 2 of 69
            Pseudo Genes (multiple problems)  :: 5 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:59:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10904128
            Orig. end                         :: 10914944
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073318.1.
            Bacteria and source DNA available from Professor Tilmann Weber,
            Technical University of Denmark, Kemitorvet Building 220, room 327D
            2800 Kgs. Lyngby, Denmark.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="50"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(40..54)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     gene            1084..1734
                     /locus_tag="JJ691_RS48995"
                     /old_locus_tag="JJ691_98110"
     CDS             1084..1734
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022805.4"
                     /locus_tag="JJ691_RS48995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98110"
                     /product="DUF3159 domain-containing protein"
                     /protein_id="WP_211766196.1"
                     /transl_table=11
                     /translation="MAAEDAEVVLSAARDRLRGLVIDVTPAFGYTVSFALTHRIGIALP
                     IALAVGIGVAAYRLARRESVWRALTVIGVVGVAGALAAGTDQAVNFYLPGLTVESLMAV
                     VTPVLLLLGWPLLGLVMAMVTGEGTGWRRCAVRRRAFTLGTLAAYGLSLVLLTIRLSLF
                     LSGQTVALGTVDVLGPFVQALGMLIGWRVYRRAVGPHRCETPSGQGETAATDR"
     gene            complement(1721..2365)
                     /locus_tag="JJ691_RS49000"
                     /old_locus_tag="JJ691_98120"
     CDS             complement(1721..2365)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099310.1"
                     /locus_tag="JJ691_RS49000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98120"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_211766197.1"
                     /transl_table=11
                     /translation="MSVELPVHTSLAGQTVTLLRELVLTGEIAAGERVNEVGLAQRLGI
                     SRGPLREAIRHLASEGLLVLTPNRGAHVPVTDDTGVHALFELRSALECAAARLAAERRT
                     DADLTRMREVSAASRAAFTTGEFPYRLDLAFHKALLDAARSPLVAEQVRLAQQRVILLR
                     AAKEVAFSHERASMDDHDTLIEALTDHDGDRAAQVMATHLDRVRDQLLTGR"
     misc_feature    2400..2418
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.98"
                     /tool="antismash"
     misc_feature    complement(2404..2422)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.81"
                     /tool="antismash"
     gene            2452..3405
                     /locus_tag="JJ691_RS49005"
                     /old_locus_tag="JJ691_98130"
     CDS             2452..3405
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800405.1"
                     /locus_tag="JJ691_RS49005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98130"
                     /product="asparaginase"
                     /protein_id="WP_211766198.1"
                     /transl_table=11
                     /translation="MHELVAEVWRGDFLESVHHGSVVAVNSDGKQVFGVGQPDDLTFPR
                     SSNKPFQALAMVRAGLDLDGELLALACASHSGEPFHIDGVRRILAGAGLTEHDLQCTAD
                     LPLGEQAQHDVLAAGGGKAPIYMNCSGKHAAMLATCVANGWPTENYLDADHPLQLAARA
                     ALEDLAGEPVGATGVDGCGAPLFAISLSGLARAFGRLAAAPAGTPEHRVARAMSQHPEW
                     VGGTGRDVTELMRGLPGAVAKDGAEGVYGIGLPDGSAVAVKIADGSSRARPVVLVAALR
                     RLGVDVAAVAAVADVPVLGHGVRVGELRPAFVMVSA"
     gene            3428..3859
                     /gene="msrB"
                     /locus_tag="JJ691_RS49010"
                     /old_locus_tag="JJ691_98140"
     CDS             3428..3859
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075655.1"
                     /locus_tag="JJ691_RS49010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98140"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_211766199.1"
                     /transl_table=11
                     /translation="MEPVVGATPKVVKSEQEWRAELTPQEYAVLREAGTERPWVGEYTD
                     TKTTGVYECRACGTELFRSDTKFDSHCGWPSFFSPLAGDRVILREDRTLGMVRVEVICA
                     TCHSHLGHVFEGEGYATPTDQRYCINSISLRLVPQTDEA"
     gene            4114..5013
                     /locus_tag="JJ691_RS49015"
                     /old_locus_tag="JJ691_98150"
     CDS             4114..5013
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="JJ691_RS49015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98150"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_211766200.1"
                     /transl_table=11
                     /translation="MGEQPWGLTGEQFLEWYLIGLGCAVALAVVIRFLPKLSAGAHHFL
                     RPTAVEIGFLAAGPQRAVETAVAELLAAGALRIESSGLLRATGAAARPSAVGAQVLARV
                     TTSHSLVDITGYLRTRPALANAGHDLAELGLIVRPHIAARFRFGSTLPVLLVLLIGVVR
                     WGNGFLLGRAIGLLTVSLVGTVVLVIAMLNFRRGRHLRTFAGDRALREFRRAPVIDAAT
                     AVALRGVHKYPDPRIAVALQRSSAPRRRPSRTGTGTGAAAGGFFFGGGGCGGGGGGGCG
                     GGGGGGCGGGGGGGGCGG"
     misc_feature    4165..4167
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5816
                     /locus_tag="JJ691_RS49020"
                     /old_locus_tag="JJ691_98160"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157007.1"
                     /locus_tag="JJ691_RS49020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98160"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_211766201.1"
                     /sec_met_domain="DUF692 (E-value: 1.4e-85, bitscore: 286.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRVTSSPRLGVGIGWRPEIDLTVERLSDVDFVEVVAENIAPGDL
                     PESLRLLRERGVPVLPHAVSLSLGGAEPLDMARVAHLAAVGQALDAPLVSDHVCFVRAG
                     GLDAGHLMPLPRTWEALDVLVANVLEAQQELPVPLALENVAALIDWPGAELTEGQFLAE
                     LVERTDCRLLVDVANLHANARNIGTDPAAFLATLPLERLAYVHVAGGVERDGLYHDTHA
                     HAMPDAVLDLLGDLCARVDPPGVLLERDDDYPSDEELAAELAAVRAVLA"
     gene            5813..6196
                     /locus_tag="JJ691_RS49025"
                     /old_locus_tag="JJ691_98170"
     CDS             5813..6196
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422375.1"
                     /locus_tag="JJ691_RS49025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98170"
                     /product="hypothetical protein"
                     /protein_id="WP_211766202.1"
                     /transl_table=11
                     /translation="MSKLASESFSAVSRDRLAAQQASLLRALLAGAPAPDGFDDNRIRA
                     QAHALLSKRSRVTWHLRPDLAAELGDRFGVLFAEYAAGHPKTVGLRARQDAEQFGLWLV
                     AQGHLTPSRRPWWRLTGRRDRIM"
     gene            6224..8341
                     /locus_tag="JJ691_RS49030"
                     /old_locus_tag="JJ691_98180"
     CDS             6224..8341
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JJ691_RS49030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JJ691_98180"
                     /product="hypothetical protein"
                     /protein_id="WP_211766203.1"
                     /transl_table=11
                     /translation="MGQRSSDDDGDRGRPEVRNEFAGTADNVIQAGTIGAVHLHVHGRA
                     REDDTDPLALAAKALAEAVRHQWTEEAGWRSLRRPEPLRVRWSVTDREVSADLATVLGE
                     GDNSVLRTQGDWRDLVEVFRALPARQLVILGEPGAGKTVLALLFVLALLDNPVAGEPVP
                     VLLSASSWNPDEHVHSWLVRRLLDEYPALGDESTYGPGAATRLVTEGWIVPVIDGLDEI
                     PVALHVAAIDAVDRVVSGGHPLVLTCRTDEYAAAVERGGLALSRALVLEIEPVGLVEAR
                     RFLCSAGPSAPRRWRPVLAAIRAEPDVPLARALASPLMVALARKVYTPTATDPRELLDT
                     ARFASQAAVERHLLDSFVTAAYSQQPERRYRLEQAREWLRFLACHLNGLDTRDLAWWEL
                     AKAVRPRTQGLLAGLSSGLIAAAAGFVADGWIGAVAYGVGAGVAVGVGHVLGHTPRPSH
                     AELRFRGTAKRFVARFMVGFAVHFVLGCAFGLDYAIASVTGLVGGCTLASHAWWSVPTD
                     ATKVSSPAITLRHERRAAVTFGVVLGVDFGFLAGLVYAFTPDLELDTIQAVLAGLGTAT
                     GIGILANLQYGRMAGVVYGVAAGVVSGLTAASAYGGPITNKVALGLAYGVAFASTVGYL
                     AVLSRAWGVFQLHRSWLALRNRLPWRLTGFLVDAHRRGVLRQAGGVYQFRHARLQERLA
                     EPEDAAPTERR"
     gene            complement(8389..9147)
                     /locus_tag="JJ691_RS49035"
                     /old_locus_tag="JJ691_98190"
     CDS             complement(8389..9147)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025377.4"
                     /locus_tag="JJ691_RS49035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_98190"
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="WP_211766204.1"
                     /transl_table=11
                     /translation="MLRERVELSMDALRAFWRWEGHDRMPAVLVSTVEWLDEDGRREAD
                     RRVLAELADRGLLAGGTVAKEVRATFHTLTRPEIEVFGWISTPQRTLGVLAAGSGGEAV
                     LMVRSGDTAQVSPVRPDDVAEVVVAQLPAVQPAHGRSVNVSETDFTGAQADSEEGFGGF
                     GNQHESPDTRALKALFKQPVLGISQFWTSARGADGRLRRAETPITVRDTAEGRWLTQTT
                     TASGRRWVIAAPAGPQLVTSKLYEVLRGLG"
     gene            complement(9181..10308)
                     /locus_tag="JJ691_RS49040"
                     /old_locus_tag="JJ691_98200"
     CDS             complement(9181..10308)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JJ691_RS49040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JJ691_98200"
                     /product="hypothetical protein"
                     /protein_id="WP_211766205.1"
                     /transl_table=11
                     /translation="MKMGAAWTGDASEAARAGAAPLLQAFRDGQQALSTHQDLMGRQTD
                     SFGVAKTSVHDVPNEPPSASFGDMVSDVSGVSVFTGGGYLNKLQDWSDKANANVAAYGS
                     YANASTYNQSNMPHEYGDPSGSASDVAVAQQQPGPGGHGPGGTGEISGPGGTGSTTHPA
                     SHYSGGPGPSAWTPSDVGNPGPGTGGLPSPGGTPGYGSGPTNTQGWPGAPSAPGTGAGG
                     WPTPGSGGQGWGSGGASGGFPGTGGVPFGMPGGGGFDGGTAGGGTGGGGARGLRAGGMA
                     GTGAGGAAAAESQTGPGARAGAGGPGGSGPGAAMAAGRPGAPGAPGAGGMAGGGRGGRK
                     EEDQEHTSAAYLEDDYSDELIGELPRTAPTVIGLD"
     misc_feature    complement(10521..10537)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.23"
                     /tool="antismash"
     misc_feature    10585..10603
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 22.16"
                     /tool="antismash"
     misc_feature    complement(10588..10600)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.78"
                     /tool="antismash"
     misc_feature    10588..10604
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.37"
                     /tool="antismash"
     misc_feature    complement(10589..10607)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 23.67"
                     /tool="antismash"
     misc_feature    10743..10754
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 ccgtggatgc cttgggggcc ttggattctc gcgaatccgt ggccgaacgg atccgctcgt
       61 tggccgccgt cgatgtggaa agccttgtgc agcaaggcgg tggggccgcc ggcgcgttgc
      121 gggcccggga gcagtggctc gctcatcccc aggctcggtt gctggagcgg ctgcccgtcg
      181 ttcagttgga acgctttgcc gacgcgccaa cggttgtgct gccgtctgcc gctcgccccc
      241 tgtcgggcgt ccgggtcctc gacctgaccc acgtcatcgc cggtcccgtg tgcggtcgaa
      301 ccctggccgc acacggggct gacgtcctgc acgtcgccgc cgcgcaccgc ccggtgatgg
      361 cggcgacgct gcgggacact tcgttcggca aacgctcgtg ctggctggac cttcggattg
      421 ccgaggaccg ccggcggctg cgggagttgg tcgccgaggc cgatgtgttc gtgcagtcct
      481 accggcacgg tgccttggcc cgtcacggtt tcgggccgga ggaactggcc gccctccgtc
      541 ccgggatcat cgccgtggac gtgtcggcgt acgggacgac cggtccgtgg ggtggccggc
      601 gcggcttcga cagcctggtg cagatggtgt ccggcatcgc cgacgacaac ggcaccccgc
      661 gatcgctgcc ggcccaagcc ctcgaccacg gctcgggctg gttggcggcc tggggcgtga
      721 tcacggcgtt gcggcggcgg gcggtcgatg gcggctcgtg gcgggtctcg gtgtcgttgg
      781 ctcggacggg gttgtggctg gattcgttgg gccgcaccgc gattccgggc agcgagcccg
      841 aggtgtcgga cctgctgacc accaccggcg agttcaccca cgtcaaggtg ccgggcacgc
      901 tcagcgccgc gccgccatac tggtcgcacg gcccacggac gcctgggggc gatccagccg
      961 cctggtggtg accgcggcaa ctcttgtcag gcgattgtca gcttcctgtc cagggaaggt
     1021 cagcgcggtt cggttcggtg gaccgtggca ccggacgaga ctgacgaacc tgatgggagc
     1081 ggcatggcgg ccgaggacgc cgaggttgtt ctgagcgctg ctcgtgatcg gctgcgcggc
     1141 ctggtgatcg acgtgacgcc ggccttcggc tacaccgtga gtttcgccct cacgcaccgg
     1201 atcggcatcg cgctgccgat cgcgctggcc gtaggcatcg gggtcgcggc ctatcggctg
     1261 gcgcgccgcg aatccgtatg gcgggccctg acggtcatcg gcgtggtcgg cgtcgcgggg
     1321 gcgctggccg ccggcaccga ccaggctgtc aacttctatc tgcccggcct cacggtggaa
     1381 agcctgatgg ccgtggtgac gccggtgctg ttgctgctcg gctggccgct gctgggcctg
     1441 gtgatggcga tggtcaccgg cgagggcacc gggtggcgcc ggtgcgcggt gcgccgccgg
     1501 gcgttcaccc tgggcaccct ggccgcatac gggttgagcc tggtgctgct gacgatccgg
     1561 ctctcgctgt tcctgtccgg ccagaccgtc gccctgggca cggtggacgt gctcggacct
     1621 ttcgtgcagg cgctcggcat gctgatcggc tggcgcgtct atcgccgcgc ggtcggcccg
     1681 caccggtgcg aaacaccttc ggggcaagga gaaaccgcgg ctaccgaccg gtgagcagct
     1741 ggtcgcggac gcggtccaga tgggtggcca tgacctgggc ggcgcggtcg ccgtcgtggt
     1801 cggtgagggc ctcgatgagg gtgtcgtggt cgtccatgga ggcccgctcg tgggagaagg
     1861 cgacctcctt ggccgcgcgc agcaggatca cccgctgctg ggcgagacgg acctgctcgg
     1921 cgaccagggg gctgcgggcg gcgtcgagca gcgccttgtg gaaggcgaga tcgagccggt
     1981 acgggaactc gccggtggtg aaggcggccc gggacgcggc gctgacctcc cgcatccgcg
     2041 tcaggtcggc gtcggtgcga cgctcggcgg ccagccgggc ggcggcgcac tccagggcgg
     2101 agcggagctc gaacagggcg tgcacgccgg tgtcgtcggt gacggggacg tgcgcgccgc
     2161 ggttgggcgt gagaaccagc agaccctcac tggccaggtg ccggatggct tcccgcagcg
     2221 ggccgcggct gatgccgagc cgctgggcca ggccgacctc gttgacccgc tccccggcgg
     2281 cgatctcgcc ggtcagcacc agctcgcgca gcagcgtcac ggtttggccg gccaagctgg
     2341 tgtgcacggg cagctcgacg ctcatgtgat gaacatccgc ctttacaacg cccacgagca
     2401 agttgttaac tgtcgacagt tgtcagttgc tgtcgagttg aggaggccgg tatgcacgag
     2461 ctcgtcgcgg aggtgtggcg gggtgacttc ctcgaatcgg tgcaccacgg ctcggtcgtc
     2521 gcggtcaaca gcgacggcaa gcaggtgttc ggcgtcgggc agcccgacga cctgaccttc
     2581 ccgcgctcgt ccaacaagcc gttccaggcg ctggccatgg tgcgcgccgg tctcgacctc
     2641 gacggtgagc tgctggccct ggcctgcgcc agccactccg gcgagccgtt ccacatcgac
     2701 ggcgtgcgcc gcatcctggc cggcgccggc ctcaccgagc acgacctgca gtgcaccgcc
     2761 gacctgccgc tgggtgagca ggcccagcac gacgtgctcg ccgccggcgg cggcaaggcc
     2821 ccgatctaca tgaactgctc cggcaagcac gcggcgatgc tcgcgacctg cgtcgccaac
     2881 ggctggccga cggagaacta cctggatgcc gaccaccccc tccagttggc cgcccgcgcc
     2941 gccctggagg acttggccgg cgagcccgtg ggcgccaccg gcgtcgacgg ctgcggggcc
     3001 ccgctgttcg cgatctcgct gagcggcctg gcccgcgcgt tcggccggct tgccgccgcc
     3061 cctgctggca ctcccgagca tcgcgtcgcc cgcgcgatga gccagcaccc cgagtgggtc
     3121 ggcggcaccg gccgtgacgt caccgagctg atgcgcggcc tgcccggcgc ggtcgccaag
     3181 gacggcgccg agggcgtcta cggcatcggc ctgccggacg gctcggcggt cgccgtcaag
     3241 atcgccgacg ggtcgagccg ggcccggccg gtggtgctgg tcgccgcgct gcgccggctg
     3301 ggcgtggacg tcgccgcggt cgccgcggtg gccgatgtcc ccgtgctcgg gcacggcgtg
     3361 cgtgtgggtg agctacgtcc cgctttcgta atggtttcgg cctgaccgga tccataaact
     3421 cttaggcatg gaaccagtcg tcggagccac ccccaaggtc gtcaagtccg agcaggaatg
     3481 gcgggccgag ctgaccccgc aggagtacgc cgttctgcgc gaggccggca ccgagcggcc
     3541 gtgggtcggc gagtacaccg acaccaagac caccggcgtc tacgagtgcc gcgcctgcgg
     3601 gaccgagctg ttccgcagcg acaccaagtt cgactcgcac tgcggctggc cgtcgttctt
     3661 ctcgccgctg gccggcgacc gcgttatcct gcgcgaggac cggaccctgg gcatggtgcg
     3721 cgtcgaggtc atctgcgcca cctgccacag ccacctcggg cacgtgttcg agggcgaggg
     3781 ctacgccacc ccgaccgacc agaggtattg catcaacagc atttctcttc gcctcgtccc
     3841 ccagacggac gaagcctgac cgaccgaccc taagccttcc tcaagaggcc ttgtccgcgc
     3901 ttttacgcgc gcccaccatg atgcgcaccc gatgccgtga aaaggcggag gggcgacgac
     3961 gtcaacgccg ccgacacctg gggaggagtc ggcgggaccg ggggtgtcgc cgggctccgc
     4021 ggctggctgg gggtcggccg cggcgcccgg tgacctcggt gcactacgca tgtccggtgc
     4081 gtctcgacca tggtcggggt acgggggtgg ggaatgggcg aacagccttg gggactgacc
     4141 ggtgagcagt tcctggaatg gtatttaatc ggtctcggct gcgcggtcgc gctggccgtc
     4201 gtgatccgat ttctgccgaa attgtcggcc ggcgcacatc atttcctccg gccgacagcg
     4261 gtggagatcg gctttctcgc cgccggcccg cagcgggcgg tcgagaccgc cgtcgccgaa
     4321 ctgctggcgg ccggcgcgct gcggatcgag agcagcggcc tgctgcgcgc caccggggcc
     4381 gccgcacggc ccagcgcggt cggcgcccag gtcctggcgc gggtgacgac cagccactcg
     4441 ctcgtcgaca tcaccggcta cctgcggacc cggcccgcgc tggcgaacgc cggccacgac
     4501 ctggccgagc tgggcctgat cgtgcggccg cacatcgccg cgcggttccg cttcggctcg
     4561 accctgccgg tgctgctcgt gctgctgatc ggggtcgtcc ggtggggcaa cggcttcctc
     4621 cttggccggg ccatcggcct gctgaccgtg tcgctggtgg ggacggtcgt gctcgttatc
     4681 gccatgctga acttccggcg cggccggcac ctgcgcacct tcgccggaga ccgggccctg
     4741 cgggagttcc ggcgcgcgcc ggtgatcgac gccgccaccg ccgtcgccct ccgcggcgtg
     4801 cacaagtacc cggacccccg gatcgccgtc gcgctgcaac ggtcctcggc acctcgccgc
     4861 cggccgagcc gtaccggcac cgggaccggg gcggctgctg gcggcttctt cttcggagga
     4921 ggcggctgcg gcggtggagg cgggggcggc tgtggtggcg gtggaggcgg tggctgtggc
     4981 ggcggtgggg gaggtggcgg atgcggcggg tgacctcgtc cccgcgactc ggggtcggca
     5041 tcgggtggcg gccggagatc gacctgaccg tcgagcgact gtccgatgtg gacttcgtcg
     5101 aggtcgtcgc cgagaacatc gcccccggtg acctgccgga gtcgttgcgg ctgctcaggg
     5161 aacgcggcgt gccggtgcta ccgcacgccg tgtccctgtc gctcggtggg gccgagccgc
     5221 tggacatggc ccgggtcgcg cacctggccg ccgtcgggca ggcgttggac gcgccgctgg
     5281 tcagcgatca cgtctgcttc gtgcgggccg gcggcctcga tgccgggcac ctgatgccgt
     5341 tgccgcggac gtgggaggcc ctcgacgtgc tcgtggccaa tgttcttgag gcgcaacagg
     5401 aactgccggt gccgctggcg ctggagaacg tggcggcgct gatcgactgg cccggcgcgg
     5461 agctgaccga gggccagttc ctggccgagc tggtcgagcg caccgactgc cggctgctcg
     5521 tggacgtcgc caatctccac gccaacgcgc ggaacatcgg caccgatccc gccgcgttcc
     5581 tggccacgct tcccttggag cgcctggctt acgtgcatgt cgccggcggc gtcgaacgcg
     5641 acggcctcta ccacgacacc cacgcccacg cgatgcccga cgccgtgctc gacctgctcg
     5701 gagacctctg cgcccgcgtc gacccgcccg gtgtgctgct cgaacgggac gacgactacc
     5761 cctccgacga ggagctcgcc gccgagctcg cggccgtccg cgccgtgctg gcatgagcaa
     5821 gcttgcgagc gaatcattca gcgcagtgag ccgcgaccgg ctcgccgccc agcaggcctc
     5881 gttgttgcgg gccctgctgg ccggcgcgcc cgcgcccgac ggcttcgacg acaaccggat
     5941 ccgtgcgcag gcgcatgcct tgctgtccaa gcgcagccgc gtcacctggc atctgcggcc
     6001 ggatctcgcc gccgagctcg gcgaccggtt cggtgtgctg ttcgccgagt acgccgccgg
     6061 ccatccgaag accgtcggcc tgcgggcgcg gcaggacgcc gagcagttcg ggctttggtt
     6121 ggtggcacag ggacacctca cgccgtcgcg tcgcccgtgg tggcgattga ccgggcgccg
     6181 ggatcgaata atgtgagggc atagatccgt gcgaggggga gcggtggggc aacgctcttc
     6241 tgacgacgac ggtgatcgtg gccggcccga agtgcgcaac gagttcgccg gcaccgcgga
     6301 caacgtgata caggccggca cgattggcgc tgtgcacctg catgtgcacg gccgtgcacg
     6361 ggaggacgac accgatccgc tggcgctagc ggccaaggcg ctggcggagg cggtccgtca
     6421 ccaatggacc gaggaagcgg gctggcgttc gttgcggcgg ccggagccgt tgcgcgtgcg
     6481 atggtcggtg acggatcggg aagtgtccgc cgatctcgcg acggtgctgg gtgaaggcga
     6541 caactccgtg ctgcggacac agggtgactg gcgcgatctg gtcgaggtgt tccgcgcgct
     6601 gccggcccgt cagctggtga tactcggcga gccgggcgcc ggcaagacgg tgctggcgtt
     6661 gctgttcgtt ttggcgttgc tcgacaatcc cgtggcgggt gagccggtgc cggtgctgtt
     6721 gtcggcgtcg tcctggaatc ccgatgagca cgtccacagc tggctggttc gccgactgct
     6781 tgacgaatac ccggcgctgg gggacgagtc cacctacggg ccgggggccg cgaccaggct
     6841 ggtcaccgag gggtggatcg tgccggtcat cgacggcctg gacgagatac cggtggcgct
     6901 gcacgtcgcc gcgatcgacg ccgtcgaccg tgtggtcagc ggtggtcatc ccctggtgtt
     6961 gacctgccgg accgacgagt acgccgcggc ggtcgaacga ggcggcctcg ccttgagtcg
     7021 ggcgttggtg ttggagatcg agccggtggg tcttgttgag gccaggcggt ttctgtgctc
     7081 cgcggggccg tcggcgccac gccgctggcg gccggtgctg gcggcgatcc gggccgagcc
     7141 cgacgtgccg ctggcgcggg cgctggccag tcccctgatg gtcgcgttgg cccgcaaggt
     7201 gtacacgccc accgccaccg atccgcgtga gctgctcgac accgccaggt tcgccagcca
     7261 ggccgcggtg gagcgacatc tgctggattc ctttgtcacg gcggcgtatt cgcagcaacc
     7321 cgagcggcgg tatcgactgg aacaggcccg cgagtggctg agattccttg cgtgccacct
     7381 gaacgggctc gacacgcgcg acctggcgtg gtgggaactg gccaaggcgg tgcggccgag
     7441 gacccagggg ctgctcgccg ggctctcctc cggcctgatc gccgctgccg cgggattcgt
     7501 cgctgatggg tggatcggcg ccgtagccta cggcgtcggg gccggcgtgg ctgtcggcgt
     7561 ggggcacgtc ctcggtcaca cgcctcgacc ctcgcacgcc gaactgaggt tccggggtac
     7621 cgcgaaacgt ttcgtcgccc ggttcatggt gggctttgcc gtccacttcg tgctggggtg
     7681 tgccttcggg ctggattacg cgatcgcctc cgtgaccggg ttggtgggcg ggtgcacgct
     7741 cgcctcccac gcgtggtgga gcgtcccgac cgatgccacg aaggtgtcgt ccccggccat
     7801 cacgctgaga catgaacgcc gggccgccgt cacgttcggc gtcgtgctgg gggtggactt
     7861 cgggttcctc gccggcttgg tctacgcgtt cacacccgat ctcgaactgg acaccatcca
     7921 ggcggtgctg gccggcctgg gcacggcgac agggatcggc atcctggcca acctgcagta
     7981 cgggaggatg gccggcgtcg tgtacggagt cgccgccggc gtcgtgtcgg ggcttaccgc
     8041 cgcctccgcc tacggcgggc ccataacgaa caaagtggcg cttggcctcg cgtacggtgt
     8101 ggcattcgcg tcgacggtcg gctacctcgc cgtgctctca agggcctggg gcgttttcca
     8161 gctccatcgg tcatggctgg cgttgcgaaa tcgtctgccg tggcgcttga cgggtttcct
     8221 cgtcgacgcg caccggcgcg gcgtgctgcg acaggctggt ggcgtgtatc agtttcggca
     8281 cgctcgcctg caggaacgct tggccgaacc cgaggatgcg gcccccaccg aacgacggtg
     8341 agtagcgtca gctggccgtg gtggcacacc ggtcagcggc ctgcgggctc agccgaggcc
     8401 gcgcaacact tcgtacagct tgctcgtcac cagttgcggg ccggccgggg cggcgatcac
     8461 ccagcgccga ccggacgccg tcgtggtctg cgtgagccag cggccctcgg ccgtgtcccg
     8521 cacggtgatc ggggtttccg ctcgccggag ccgcccgtcg gcgccgcggg ccgacgtcca
     8581 gaactggctg atgcccagca ccggctgctt gaacaaggct ttcaacgccc tcgtgtcggg
     8641 cgactcgtgc tggttgccga atccgccgaa tccctcttcg ctgtcggctt gcgccccggt
     8701 gaagtccgtc tcggacacgt tcaccgatcg gccgtgggcg ggctgcaccg ccgggagttg
     8761 cgcgaccacc acctcggcca cgtcgtcggg gcgcaccggc gacacctggg ccgtgtcacc
     8821 cgagcgcacc atcagcactg cctccccgcc gctgcccgcg gccagcacgc ccagggtcct
     8881 ctgtggagtg ctgatccagc cgaacacctc gatctcgggc cgggtcagcg tgtggaaggt
     8941 ggcgcgcacc tccttggcca ccgtgccgcc ggcgagcagg ccccggtcgg ccagctcggc
     9001 caacacccgc cggtcggcct cgcggcggcc gtcctcgtcc agccattcca ctgtggacac
     9061 cagcaccgcc ggcatgcggt cgtggccttc ccagcgccag aacgccctca gcgcgtccat
     9121 ggacaactcg acgcgctctc gtagcacgcc aacccccagt tggttccgat cttgtggtcg
     9181 tcagtccagg ccgatcaccg ttggcgcggt gcgggggagt tcgccgatga gctcgtcgga
     9241 gtagtcgtcc tccaggtagg ccgccgacgt gtgctcctgg tcctcctcct tccggccgcc
     9301 ccgaccgccg ccggccatgc cgccggcgcc cggcgcccca ggcgctcccg gcctgcccgc
     9361 cgccatcgcc gcgcccggac cgctcccgcc gggaccgccc gcgccggcgc gggccccggg
     9421 gcccgtctgg ctctcggccg ccgccgcgcc accagctccc gttccggcca tgccgccggc
     9481 tcggaggcct cgtgcacccc cgcctcccgt gccaccaccg gccgttccgc cgtcgaaccc
     9541 gccgcctccg ggcatgccga acggcacgcc acccgttccc gggaagccgc cgctggcgcc
     9601 tccagagccc cagccctggc caccgctacc gggcgtcggc cagccgccgg caccggtgcc
     9661 cggcgcggac ggtgcgcccg gccagccctg ggtgttcgtc ggtccgctcc cgtagcccgg
     9721 agtaccgccc gggctcggca gtccgcccgt tcccggtccc gggttgccga catcggacgg
     9781 cgtccacgcc gatgggcccg ggccgccgct gtagtgcgag gccgggtgcg tcgtgcttcc
     9841 tgtgccaccg ggtccgctga tctcgcccgt gccgcccgga ccatggccgc ccggccccgg
     9901 ctgctgctgg gccaccgcga cgtccgaggc cgatccactc gggtcgccgt actcgtgcgg
     9961 catgttgctc tggttgtacg tgctggcgtt cgcgtagctg ccgtacgccg cgacgttggc
    10021 gttggccttg tccgaccagt cctgcagctt gttcaggtag ccgcccccgg tgaacacgct
    10081 caccccgctc acgtcgctca ccatgtcccc gaacgacgcc gacggcggct cgttcggcac
    10141 gtcgtgcaca ctcgtcttgg ccaccccgaa cgagtccgtc tgccgcccca tcagatcctg
    10201 atgagtgctc aacgcctgct gcccgtcccg aaacgcctgc aacaacgggg cagctccggc
    10261 ccgcgccgcc tccgacgcat cccccgtcca cgccgcaccc atcttcatga ccaacgccaa
    10321 agcctggttc tcacggtcct gatggctctg cgccagcgaa gtagaggccg cccgcgcatc
    10381 ttgaaaactg ctggtacccg gcccctgcgt cagggcgctg tggatgtcac ccggccctag
    10441 tccacccatt acgcccctcc cttgatggtc gtgatctcca tgtcggcggc cgtcttggcg
    10501 atcgtgcacg ggtcatgctg ttgcgaggga tttgtgacac tgacggtaac ggtgaactgc
    10561 aacgcgtcac tcacgccaac ccctagattg catacggcat tcaacttagc ctttcgatca
    10621 tcgaccaggg agtacagcac aactggatac ccttggatcg ggtcaatcgg ctcgaagtat
    10681 ccgccgttct tcttgagcga ttcggcctgg acgtacagtg agcttaggcc gccggtgctc
    10741 ttggaaatga acccgctgcc gacgctgatg ggtgtaggaa ccgctgaagt gtcgtgccaa
    10801 tcgcaagctg ggccgt
//
