LOCUS       NZ_CP073318            10402 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Kutzneria sp. CA-103260 chromosome, complete genome.
ACCESSION   NZ_CP073318
VERSION     NZ_CP073318.1
KEYWORDS    RefSeq.
SOURCE      Kutzneria sp. CA-103260
  ORGANISM  Kutzneria sp. CA-103260
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kutzneria.
REFERENCE   1  (bases 1 to 11609901)
  AUTHORS   Jorgensen,T.S., Gren,T., Kontou,E.E., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Complete Genome Sequence of the Rare Actinobacterium Kutzneria sp.
            Strain CA-103260
  JOURNAL   Microbiol Resour Announc 10 (30), e0049921 (2021)
   PUBMED   34323613
REFERENCE   2  (bases 1 to 11609901)
  AUTHORS   Joergensen,T.S., Gren,T., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2021) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-APR-2021
            Assembly Method :: Flye v. 2.8-b1674; unicycler_polish v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:18:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,326
            Genes (coding)                    :: 10,257
            CDSs (with protein)               :: 10,257
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 9 of 69
            Pseudo Genes (incomplete)         :: 63 of 69
            Pseudo Genes (internal stop)      :: 2 of 69
            Pseudo Genes (multiple problems)  :: 5 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:59:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6366219
            Orig. end                         :: 6376621
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073318.1.
            Bacteria and source DNA available from Professor Tilmann Weber,
            Technical University of Denmark, Kemitorvet Building 220, room 327D
            2800 Kgs. Lyngby, Denmark.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="39"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5402)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(160..1404)
                     /locus_tag="JJ691_RS27925"
                     /old_locus_tag="JJ691_55790"
     CDS             complement(160..1404)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="JJ691_RS27925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55790"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_211762591.1"
                     /transl_table=11
                     /translation="MLAPARLNVVLQHQNRLVRELMATHLTREPGVTLAGTVASAPELV
                     QLCRLCRPEVAVFQADPPRWSNDRLVSLLLEPGRRLRMVGVHEMLPAAYVIRAYEAGIS
                     ALVSSNRGLDALVEAIKVPSAAVEITRLDKGRRQALTTRELEVLYLISAGYAPRQVAAE
                     LGISPHTVEHHKQRIFAKLDVHNQAHAAASATRLGLMASVIELPRQERGQDLLRVCVLT
                     RDEGCLLADRVRRILTEHDIPVVGADEPGVATVLVDPEPGDWQALTGGRSAPVVVASRQ
                     LPLQHGLEVLAAGITVLPAASVDGMIVPAVRAAGQGYLMVHAVNSRAMLGVARGADRTW
                     WRWQLSLTRREQEIIASIGRGHSAKQTARLLGISVRTVENLQSSLFRKLGVHSKAAALA
                     AAQDLGLLEESDGVS"
     gene            1681..2058
                     /locus_tag="JJ691_RS27930"
                     /old_locus_tag="JJ691_55800"
     CDS             1681..2058
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /locus_tag="JJ691_RS27930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55800"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_211762592.1"
                     /transl_table=11
                     /translation="MRMRFHWILPAETDPVTFCRTAEAAGIDQALLPAAFQRPDPVAPA
                     THIASATKEIGLMVCPRTDMRSPTHLVRQVNTVSAVTNGRVSVVGVASLAGSARGPNRL
                     VCPPRLNSACFGGAARFPGVR"
     gene            2097..2978
                     /locus_tag="JJ691_RS27935"
                     /old_locus_tag="JJ691_55810"
     CDS             2097..2978
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /locus_tag="JJ691_RS27935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55810"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_211762593.1"
                     /transl_table=11
                     /translation="MTRLLVAALATLAAVATAPAAFASIGPLHVVAMGDSYASGTGAGD
                     YEAGTEGNCWRSANSYSEQVVARLRARGTSVQFTNVACSGAATSDLHQEFLGQPPQLDA
                     LQVDTAIVLLTIGTNDIGYAAYGGLCIQGDCSGAPTQAITAELPDMAKNLAQLFRDIHD
                     RSPHAKIVLTGYGSQLTAGPNADGQLDPICDAGVFSPQERAQGNVLASQLDKTLRDTTA
                     QAGPDTGYVSEYAADSTHLVDAFAGHSLCQAGTPFYRGFDALTPGQEGPDAVLHLNKDG
                     QSALADLVLSQV"
     gene            complement(2984..4000)
                     /locus_tag="JJ691_RS27940"
                     /old_locus_tag="JJ691_55820"
     CDS             complement(2984..4000)
                     /NRPS_PKS="Domain: PKS_ER (18-232). E-value: 5.2e-11.
                     Score: 34.1. Matches aSDomain:
                     nrpspksdomains_JJ691_RS27940_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225362.1"
                     /locus_tag="JJ691_RS27940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55820"
                     /product="alcohol dehydrogenase"
                     /protein_id="WP_249227060.1"
                     /sec_met_domain="ADH_N (E-value: 2.2e-28, bitscore: 97.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4e-18, bitscore:
                     64.9, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTYRAYEVTGKRDFRLVERELVDPAPGQVRIRVEANGVCHSDAV
                     TVEGQRPDPSVPVVPGHEIVGVIDAIGEGVRIWQVGDRVGVGFLGGHCGVCEFCRRGDF
                     VNCADQPWTGMSVDGGYAEVAYAKASGLVRVPDVLRAVDAAPLVCAGITVFTAIREINA
                     RPGALVAVQGIGGLGHLAVQQANKLGYEVAAIARGTDKAQLAAELGAHHYIDSTVKDPA
                     AALKRLGGAAGIVATASNVASMSPLVAGLAPRGQLLVVGASADPLSVQGLDLVFGTRTV
                     KGSLTGTSIENEDNLKFAAAQGIRPMVEVLPLAEAPKAYEHMMSGAARFRVVLDTTS"
     aSDomain        complement(3305..3946)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJ691_RS27940_PKS_ER.1"
                     /evalue="5.20E-11"
                     /label="JJ691_RS27940_PKS_ER.1"
                     /locus_tag="JJ691_RS27940"
                     /protein_end="232"
                     /protein_start="18"
                     /score="34.1"
                     /tool="antismash"
                     /translation="ERELVDPAPGQVRIRVEANGVCHSDAVTVEGQRPDPSVPVVPGHE
                     IVGVIDAIGEGVRIWQVGDRVGVGFLGGHCGVCEFCRRGDFVNCADQPWTGMSVDGGYA
                     EVAYAKASGLVRVPDVLRAVDAAPLVCAGITVFTAIREINARPGALVAVQGIGGLGHLA
                     VQQANKLGYEVAAIARGTDKAQLAAELGAHHYIDSTVKDPAAALKRLGGAA"
     misc_feature    complement(4079..4094)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     gene            complement(4114..5004)
                     /gene="thpD"
                     /locus_tag="JJ691_RS27945"
                     /old_locus_tag="JJ691_55830"
     CDS             complement(4114..5004)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="JJ691_RS27945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55830"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_211762595.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-35, bitscore: 123.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAVTDRYPTRGRAPAGPIPRTEPVVWTGPGTGPIPDAVVDSYD
                     SSGFLAIERLISPAEVQALRQELARLCADEAVRRDERAVVEKDSAAVRSIFEVHKISEP
                     IAALARDPRILGRVRQILGSEVYVHQSRVNLMPGFTGKGFYWHSDFETWHAEDGMPLMR
                     AVSVSIALTDNFPYNGGLMLMPGSHRTFVPCPGETPDDNYRESLREQEIGVPSQQALTE
                     LATTHGIEQFTGPAGSALMFDSNCMHGSGSNITPFPRSNVFLVFNSVDNALTDPFGAPR
                     PRPDFVAGRDFTPLG"
     gene            complement(5001..5402)
                     /locus_tag="JJ691_RS27950"
                     /old_locus_tag="JJ691_55840"
     CDS             complement(5001..5402)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156386.1"
                     /locus_tag="JJ691_RS27950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55840"
                     /product="ectoine synthase"
                     /protein_id="WP_211762596.1"
                     /sec_met_domain="ectoine_synt (E-value: 7.2e-54, bitscore:
                     180.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLHDLDGTNRDVVTPNWRSKRIILAGDGVGFSMHETVLKAG
                     TVNDFWYANHVEAVLCVEGEGELTDKETGEVFQLRPGTLYLLDGHERHQVRPRTDIRTV
                     CVFNPPVTGREVHDENGVYPLIRSEELPT"
     gene            complement(5399..6643)
                     /gene="ectB"
                     /locus_tag="JJ691_RS27955"
                     /old_locus_tag="JJ691_55850"
     CDS             complement(5399..6643)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-355). E-value: 5.9e-67.
                     Score: 218.0. Matches aSDomain:
                     nrpspksdomains_JJ691_RS27955_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787752.1"
                     /locus_tag="JJ691_RS27955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55850"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_211762597.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.2e-69, bitscore:
                     232.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFDTLESEVRSYCRSWPVVFDQAVGSTLSDEQGRQYLDFFAGA
                     GALNYGHNNPLLKQPLLEYLAADRVVHSLDMNTVAKREFLETFEELVLRPRDLDYRVQF
                     PGPAGNNAVEAALKLARKVTGREAVVNFTNAFHGMTLGALSVSGGSMKRGGAGIPLVHA
                     TPMPYDSYFDGQYPDFLYFERLLDDKGSGLNRPAAVIVETVQGEGGINTARFEWLAGLA
                     DLCRRHDILLIVDDVQMGCGRTGPFFSFEAAGITPDIVCLSKSIGGYGLPMALTLMRPD
                     LDVWEPGEHNGTFRGVNPSFVTAKAALEHYWADDSLEKGTLAKGEQVGRALLELTQTVD
                     GVEARGRGLARGLAFQRTELGNQVAAAAFERGLLVETSGPDSEVAKLMPPLTITDDELA
                     RGLDVFADAVRAVAA"
     aSDomain        complement(5579..6577)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJ691_RS27955_Aminotran_3.1"
                     /evalue="5.90E-67"
                     /label="JJ691_RS27955_Aminotran_3.1"
                     /locus_tag="JJ691_RS27955"
                     /protein_end="355"
                     /protein_start="22"
                     /score="218.0"
                     /tool="antismash"
                     /translation="DQAVGSTLSDEQGRQYLDFFAGAGALNYGHNNPLLKQPLLEYLAA
                     DRVVHSLDMNTVAKREFLETFEELVLRPRDLDYRVQFPGPAGNNAVEAALKLARKVTGR
                     EAVVNFTNAFHGMTLGALSVSGGSMKRGGAGIPLVHATPMPYDSYFDGQYPDFLYFERL
                     LDDKGSGLNRPAAVIVETVQGEGGINTARFEWLAGLADLCRRHDILLIVDDVQMGCGRT
                     GPFFSFEAAGITPDIVCLSKSIGGYGLPMALTLMRPDLDVWEPGEHNGTFRGVNPSFVT
                     AKAALEHYWADDSLEKGTLAKGEQVGRALLELTQTVDGVEARGRGLARGLAF"
     gene            complement(6640..7143)
                     /gene="ectA"
                     /locus_tag="JJ691_RS27960"
                     /old_locus_tag="JJ691_55860"
     CDS             complement(6640..7143)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787753.1"
                     /locus_tag="JJ691_RS27960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55860"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_211762598.1"
                     /transl_table=11
                     /translation="MLDSDQVAIERPSPVDGRELWRLARDSAVLDLNSSYAYLLWTRDF
                     ADTSAVARGAAGAVGFVSGYRRPDGLDTLMVWQVAVAESHRGRGLARAMLDELVGRVRP
                     RFLETTIAPANDASIRLFTSLARAHDVELARSALFTPELFPDEHEAEQLYRIGPWPNNP
                     RDSS"
     misc_feature    complement(7188..7203)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    complement(7215..7228)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.91"
                     /tool="antismash"
     misc_feature    complement(7216..7229)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.75"
                     /tool="antismash"
     misc_feature    7244..7255
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.43"
                     /tool="antismash"
     misc_feature    7260..7275
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.6"
                     /tool="antismash"
     gene            7447..10107
                     /locus_tag="JJ691_RS27965"
                     /old_locus_tag="JJ691_55870"
     CDS             7447..10107
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099780.1"
                     /locus_tag="JJ691_RS27965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_55870"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_211762599.1"
                     /transl_table=11
                     /translation="MTVRFRLLGEIRVLRDDQPVDIGHRRLRCLLAALLVDVGRVVPLD
                     TLVDRMWREQAPGRPRDTLYTYMSRLRTVLAGIENVGLAQRSGGYVLTADADSVDLHRF
                     RRLRAEATAAGDDATMADRLSRALAEWDGEPFAGTTSDWLDEQRTLLTTEHLAARLDFA
                     EVRLRRGEHAGVLAALAALAAEHPLDERLSGLLMLALGRNGRQAEALREYDRIRRLLAE
                     ELGTDPGPALQQLHRQVLAGEIALPDQFPVPHQLPGVGRFVGRSALLAALDRPSTMVTV
                     LHGPGGIGKTQLALEWAHRNAERHPDGQLFVDLRGFAAAGEPMAAQVAVRGFLDAFGVA
                     PARIPVGFDAQVGLYRSLVAGRRLLIVLDNAVDTAQVAPLLPGTPTCTVLVTSRDRLPG
                     LVTAHGARALAVDVLTAEESRELLTGRVGSAEPVAVQDLLSRCGGFPLALSIVAGRAEA
                     QPDFPLAVLADELRESGLDALDDADPAASLPAVLARSYVALPPEQAKVFGLLGLAPGPD
                     IGLAAAAALTGLPPARTRSVLRALQRVSLLHQDVPDRWRLHDLIALYAADQAGSDDVAL
                     RRLVDSCVRTACGGDHLLDPHRQPIEVEPLLLPDKDSALAWFTAEHACLLAALQLAVRR
                     GWHLEVWRLAWALTTFHRRQGHLSVNLDCWLLALDAADQVGDPGLRQRTHRLLGRAFAR
                     VGSNARALFHLRQSLELAEDVPERAHSHIALARALARAGDDEQALSHATDALALYRELA
                     APAWEATALNEIGLISARVGKLDDARLACEAALLLHREQGNRDGEANTLDNLGRLGSDV
                     AAVRHYEQALALYRELGNTHDEADTLDRLAERHLGLGDRARASARWREALALYQAQFRD
                     ADSDRVRRRLGSFGR"
ORIGIN
        1 cgtctacatg acctgcctga ccagtcacgg cgtcgcggtg cacgcgatcg aaccgttcgg
       61 ctccggctgg acctacgacg acggcgtgac gcagcggctc accgaggcac agcagcagaa
      121 ggtcgaccac gactgccagc tggcctcgtt caagctgaac tagctgacac cgtcggattc
      181 ctccagcagc cccagatcct gggccgcggc cagcgccgcc gccttgctgt gcacgccaag
      241 cttgcggaac aggctgctct ggaggttttc cacggtacgc acggagatcc cgagcaggcg
      301 ggcggtctgc ttggccgagt gaccgcggcc gatggaggcg atgatctcct gttcccgccg
      361 ggtcagcgag agctgccacc gccaccaagt acggtccgca ccacgtgcga cgcccagcat
      421 ggcccgggaa ttcaccgcgt gcaccatcag atagccctgg ccggccgccc gcaccgccgg
      481 cacgatcatc ccgtcgacac tggccgcggg cagcacagtg atgcccgccg ccagcacctc
      541 cagcccgtgt tgcaggggaa gttgccgact ggccaccacc accggggccg agcggccgcc
      601 ggtaagcgct tgccagtccc cgggttccgg gtcgaccaac accgtggcca cccccggctc
      661 gtcggcgccg acgaccggga tgtcgtgctc ggtcaggatt cgccgcaccc ggtcggcgag
      721 caggcagccc tcgtcgcgcg tcagcacgca cacgcgaagc agatcctggc cgcgttcctg
      781 ccggggcagt tcgatcaccg aggccatcag ccccagccgg gtggcacttg ccgccgcgtg
      841 agcctggttg tgcacgtcca acttggcgaa gatgcgctgc ttgtggtgtt ccacggtgtg
      901 cgggctgatg ccgagctcgg cggcgacctg ccggggcgcg tagccggcgc tgatcaggta
      961 cagcacctcc agctcgcgag tggtcagcgc ctgacgtctt cccttgtcca gccgggtgat
     1021 ctccaccgcc gccgacggca ccttgatcgc ctcgaccaac gcgtccaggc ctcggttgga
     1081 cgacaccagc gcgctgatgc cggcctcgta ggcccggatc acgtaggccg ccggcagcat
     1141 ctcgtgcacg ccgaccatcc gcagccgccg tcccggctcc agcagcagcg acaccagccg
     1201 gtcgttgctc cagcgcggcg ggtcagcctg gaagaccgcc acctcgggcc ggcacagccg
     1261 gcacagttgg accagttcgg gggccgaggc cacggtgccg gccagcgtga cgccgggctc
     1321 cctcgtcagg tgcgtcgcca tcagttccct gaccagccgg ttctggtgct ggaggacgac
     1381 gttgagccga gccggtgcca gcattgtcga ttctccctcc gccgccccga cgaggtatgg
     1441 cctgcaagcg attacatcgg cccttcgccc atggctcctc cgcgattagc atgacgagag
     1501 cacatcatgt ggcctgttgg tggcggtcaa cgagaatcca ctcgacggcg gctgattatc
     1561 gcagcgacgc gtgccaccgg caacaaatcg gccgtcgatt acctaggtat tccaaaggca
     1621 cggacacctg gggagaacaa gccataccgg cgccggccgc ggccgaccta gcatgcggcg
     1681 atgcgcatgc ggttccactg gattctgccg gccgagaccg atccggtgac gttctgccga
     1741 acggccgagg cggccggcat cgaccaggcg ctgctgccgg cggcgttcca acgacccgac
     1801 ccggtcgcac cggccacgca catcgccagt gccaccaagg agatcgggct gatggtctgc
     1861 ccgcgcaccg acatgcggtc gccgacccac ctggtgcggc aggtgaacac ggtgtcagcg
     1921 gtgaccaacg ggcgggtgag cgtggtcggc gtggcctcac tggccggttc ggcgcgcggg
     1981 ccgaaccggc tggtgtgccc gccgcggctg aactccgcgt gtttcggcgg tgccgcccgg
     2041 tttccgggag tacggtgacg atcactcggc agtcacgtac gagcacggga gcgaagttga
     2101 cacgacttct cgtcgccgcc ctggccacgc tcgctgccgt cgccacggcc ccggcggcct
     2161 tcgcttccat cggtccgctg cacgtggtgg ccatgggtga ctcgtacgcg tcgggcaccg
     2221 gcgccggcga ctacgaggcc ggcactgagg gcaattgctg gcgcagcgcc aactcctact
     2281 cggagcaggt cgtcgcccgg ttgcgggcgc ggggcaccag cgtgcagttc accaatgtcg
     2341 cgtgcagtgg cgcggcgact tccgatctgc accaggagtt tctgggccag ccgccgcagc
     2401 tggacgcgtt gcaagtggac accgctatcg tgctgctgac catcggcacc aacgacatcg
     2461 gctacgccgc gtacggcggg ctgtgcatcc agggcgactg ctccggcgcg ccgacccagg
     2521 ccatcacggc cgaactaccg gacatggcca agaacctggc ccagctgttc cgggacatcc
     2581 acgaccgcag cccgcacgcc aagatcgtgc tgacgggcta cggcagccag ctcaccgccg
     2641 gcccgaacgc cgacggccag ctcgacccga tctgcgacgc cggcgtgttc agcccgcagg
     2701 aacgggccca gggcaacgtt ctggccagcc agctggacaa aacgttgcgt gacaccaccg
     2761 cccaggccgg gcccgacacc gggtacgtca gcgagtacgc cgccgactcc acccacctcg
     2821 tcgacgcctt cgccggacat tcgctgtgcc aggccggaac tccgttctac cgcggcttcg
     2881 acgcgctgac ccccggccag gaggggccgg atgccgtgct gcacctgaac aaggacggcc
     2941 agtccgcact ggccgacctc gtgctgagcc aggtctgagc cgatcagctg gtggtgtcca
     3001 gcaccacccg gaaccgggcc gcgccggaca tcatgtgctc gtaggccttc ggggcctcgg
     3061 ccagcggcag cacctcgacc atcggccgaa ttccctgcgc ggcggcgaac ttcaggttgt
     3121 cctcgttctc gatcgacgtg ccggtgaggc tgcccttcac cgtgcgcgtg ccgaagacca
     3181 ggtcaagacc ctgcacggac agcggatcgg cggacgcgcc gaccaccagc agctggccgc
     3241 gcggggccag gccggccacc agcggggaca tcgacgccac gttggacgcg gtggccacga
     3301 tgccggcggc gccgccgagg cgcttgagcg cggccgcggg gtccttgacg gtgctgtcga
     3361 tgtagtggtg cgcgccgagt tcggcggcca gctgcgcctt gtcggtgccg cgggcgatcg
     3421 ccgccacctc gtagccgagc ttgttggcct gctggacggc caggtgcccg aggccgccga
     3481 tgccctggac cgccaccagc gcgccgggcc gggcgttgat ctcgcggatc gccgtgaaga
     3541 cggtgatgcc ggcgcacacc agcggggcgg cgtccaccgc gcgcagcacg tccggcaccc
     3601 ggaccaggcc gctggccttg gcgtaggcca cttccgcgta gccgccgtcc accgacatgc
     3661 cggtccacgg ctggtcagcg cagttcacga agtcgccgcg gcggcagaat tcgcacaccc
     3721 cgcagtgccc gccgagaaag ccgacgccca cgcggtcgcc gacctgccag atccgcacgc
     3781 cctcgccgat cgcgtcgatc acgccgacga tctcgtggcc cggcaccacc ggcacgctcg
     3841 ggtcgggccg ctggccctcg accgtcaccg cgtcggagtg gcagacgccg ttggcctcca
     3901 cccggatccg cacctgaccc ggcgccgggt ccaccagttc ccgttccacc agccggaagt
     3961 cccgcttccc ggtcacctcg tacgcccgat acgtgctcac ggccagctcc ccttcgttca
     4021 ttgaaaatga ccggtcgtct cagaccaact atgcaccacc gcgcacctgt gcgccaaccg
     4081 tgaccgtggt cacggctggc gcacaggggg agctcagccg agcggcgtga agtcccggcc
     4141 ggccacgaag tccggccgcg gccgcggcgc gccgaacggg tcggtcaggg cgttgtccac
     4201 gctgttgaac accaggaaga cgttcgaccg cgggaacggc gtgatgttgc tgccggagcc
     4261 gtgcatgcag ttcgagtcga acatcagcgc cgacccggcc gggccggtga actgttcgat
     4321 gccgtgcgtg gtggccagtt cggtcagcgc ctgctggctc ggcacaccga tctcctgctc
     4381 ccgcagggac tcccggtagt tgtcgtccgg cgtctcgccc gggcagggca cgaaggtccg
     4441 gtgcgagccg ggcatcagca tcagcccgcc gttgtacggg aagttgtcgg tcagcgcgat
     4501 ggacacactc accgcccgca tcagcggcat gccgtcctcg gcatgccagg tctcgaagtc
     4561 ggagtgccag tagaagccct tgccggtgaa gccgggcatc aggttcaccc ggctctggtg
     4621 cacgtagacc tcggagccga ggatctgccg cacccgcccc aggatccgcg gatcccgggc
     4681 cagcgcggcg atcggctcgc tgatcttgtg cacctcgaag atcgagcgca cggcggccga
     4741 gtccttctcc accacggccc gctcgtcccg gcgcaccgcc tcgtcggcgc acagccgggc
     4801 cagctcctgg cgcagcgcct gcacctcggc cgggctgatc agccgctcga tggccagaaa
     4861 gccggagctg tcataggaat ccaccaccgc gtcgggaatg ggcccggtgc ccggcccggt
     4921 ccagaccacc ggctcggtcc gcgggatcgg gccggccggc gcccggccgc gggtcggata
     4981 gcggtcggtg acagcggttg tcatgtcggc agctcctcac tgcgaatcag tggatacacc
     5041 ccgttctcgt cgtgcacctc gcgtccggtg accggcgggt tgaacacgca cacggtgcgg
     5101 atgtcggtgc ggggccgcac ctggtgccgc tcgtgcccgt cgagcaggta gagggtgccc
     5161 gggcggagct ggaagacctc gccggtctcc ttgtcggtca gctcgccctc accctcgacg
     5221 cacagcaccg cctcgacgtg gttggcgtac cagaagtcgt tgacagtgcc ggctttcagc
     5281 acggtctcgt gcatggagaa gccgaccccg tcgccggcca gaatgatgcg cttgctgcgc
     5341 cagttcgggg tcacgacgtc gcggttggtg ccgtcgaggt cgtgcagcga gcggacgatc
     5401 acgcggccac cgcccgcacc gcgtcggcga acacgtccag gccccgggcc agctcgtcgt
     5461 cggtgatggt cagcggcggc atcagcttgg ccacctcgct gtccggcccg gacgtctcga
     5521 ccagcagccc gcgctcgaac gcggccgccg ccacctgatt gcccagttcg gtgcgctgga
     5581 aggccagtcc ccgggccagc ccgcggccac gagcctccac accgtccacg gtctgggtca
     5641 gctccagcag agcccggccg acctgctcgc ccttggccag cgtgcccttc tccagcgagt
     5701 cgtcggccca gtagtgctcc agcgccgcct tcgccgtgac gaacgacggg ttgaccccgc
     5761 ggaacgtccc gttgtgctcg ccgggctccc agacgtccag gtccgggcgc atcagcgtga
     5821 gcgccatggg caggccgtag ccgccgatcg acttggacag gcacacgatg tccggcgtga
     5881 tgccggcggc ctcgaagctg aagaacggcc ctgttcggcc gcagcccatc tgcacgtcgt
     5941 cgacgatcag caggatgtcg tgccggcgac acaggtcggc cagcccggcc agccactcga
     6001 agcgggcggt gttgatgccg ccctcgccct gcaccgtctc cacgatcacc gcggccgggc
     6061 ggttcaggcc gctgcctttg tcgtcgagca gccgctcgaa gtacaggaag tccgggtact
     6121 ggccgtcgaa gtagctgtcg tagggcatcg gggtggcgtg caccagcgga atgccggccc
     6181 cgccgcgttt catcgagccg ccgctgaccg acagcgcgcc cagggtcatg ccgtggaagg
     6241 cgttggtgaa gttgacgacc gcctcacggc cggtcacctt gcgggccagc ttcagcgcgg
     6301 cctcgacggc gttgttgccg gccggaccgg ggaactgaac ccggtagtcc aggtcccggg
     6361 gccgcagcac cagttcctcg aaggtctcca ggaactcgcg cttggccacg gtgttcatgt
     6421 ccagcgagtg cacgacccgg tcggccgcca ggtactccaa cagcggctgc ttgagcagcg
     6481 ggttgttgtg gccgtagttg agcgctcccg cgccggcgaa gaagtcgagg tactgccggc
     6541 cctgctcgtc gctcagcgtg ctgccgacgg cctggtcgaa caccaccggc caggaccggc
     6601 agtagctgcg cacctcggat tcgagggtgt cgaagatgct cacgatgagt cccttggatt
     6661 gttcggccac ggtccgatgc ggtacagctg ctcggcctcg tgctcgtccg gaaaaagctc
     6721 gggagtgaac agcgcggagc gcgccagttc gacgtcgtgg gcccgggcca gcgaggtgaa
     6781 caggcgaata gaggcgtcgt tggccggggc gatggtcgtt tccaggaacc ggggccgcac
     6841 ccgaccgacg agttcgtcga gcatcgcccg ggccaggcca cgcccccggt gcgactcggc
     6901 caccgcgacc tgccacacca tcagcgtgtc caggccatcc gggcgccggt accccgacac
     6961 gaacccgacg gcgccggccg cgccgcgggc caccgccgag gtgtccgcga aatcccgcgt
     7021 ccacaacaga taggcgtacg acgagttgag atcgagcacc gccgaatcgc gggcgagacg
     7081 ccacagctca cgaccgtcca ccggggacgg ccgttcgatt gccacctgat cgctgtcgag
     7141 cattcgaccg aacgtagcag cgaattcgaa aggagaacac agtggcttgt gattcggatc
     7201 acaaatgttg cgggtttatt tcatacattc gttggggccg accggaaacg aattcgcact
     7261 gcgacggtgg tcacatcgtc cgttgtggac ggtggtttgc cgcggccgga taacgggaat
     7321 cgcgcgcgcg ttgactgccg ctgccccagg gccccgactc cctggggcag cgggacgggc
     7381 cgcctcccgt ccgcgacgag cgtgccggat cgagcttgca aggacccttc acgtaccttg
     7441 ccggtggtga cggttcggtt ccggctgctc ggtgagatcc gggtgctgcg cgacgaccag
     7501 cccgtggata tcgggcaccg gcggctacgc tgcctgctcg cggcgctgct ggtggacgtc
     7561 ggccgggtgg tgccgctgga caccctggtg gaccggatgt ggcgcgagca ggcccccggc
     7621 cgtccgcgcg acacgctgta cacctacatg tcccggctgc ggaccgtgct ggccggcatc
     7681 gagaacgtcg gcctggccca gcgttccggc ggctacgtgc tgaccgccga tgccgattcg
     7741 gttgatttgc accgttttcg ccggcttcgg gccgaggcca cggcggccgg cgacgacgcc
     7801 acgatggccg accggctgag ccgggcgctg gccgagtggg acggggaacc gttcgccggc
     7861 acgaccagcg actggctcga cgagcagcgc accctgctga ccaccgagca ccttgcggcc
     7921 cggctggact tcgccgaggt gcggcttcga cgtggcgaac acgctggcgt gctggccgcg
     7981 ctggcggcat tggctgcgga gcatcccctg gacgaacggt tgagcggact actcatgctc
     8041 gccctgggcc gcaacggccg ccaggccgag gcgttgcggg agtacgaccg gatccgccgg
     8101 ctgctggccg aggagctggg cacggatccc ggtccggcgt tgcaacagct gcaccggcag
     8161 gtgctcgccg gcgagatcgc cctgcccgac cagttcccgg taccgcacca actgcccggt
     8221 gtcggccgct tcgtcgggcg gtcggccctg ctggcggcgc tggaccgacc gtccacaatg
     8281 gtgactgtgc tgcacgggcc gggcggcatc ggcaagaccc agctggcgct ggaatgggcc
     8341 caccgcaacg ccgagcgcca tcccgacggg cagctgttcg tcgacctcag gggattcgcc
     8401 gccgccggcg agccgatggc cgcccaggtg gccgtgcggg gcttcctgga cgccttcggc
     8461 gtcgcgccgg cgcggatccc ggtcggcttc gacgcgcagg tcgggctgta ccggagcctg
     8521 gtcgccggtc gacgcttgct catcgtgctg gacaacgcgg tcgacaccgc gcaggtggcg
     8581 cccctgctac ccggcacgcc gacgtgcact gtgctggtca ccagccggga tcggctgccc
     8641 ggcctggtca ccgcgcacgg ggcccgtgcg ctggccgtgg acgtgctcac tgcggaggag
     8701 tcccgggaac tgctgaccgg tcgggtcggc tcggccgaac ccgttgcggt gcaggacttg
     8761 ttgtcccgct gtggcggttt cccgctggcg ctgagcattg tcgccgggcg ggccgaggcg
     8821 cagcccgact tcccgctggc ggtgctggcc gacgagttgc gggagtccgg tttggacgcc
     8881 ctcgacgacg ccgacccggc cgccagcctg cccgcggtgc tggcccgctc gtacgtcgcg
     8941 ctgccaccgg agcaggcgaa ggtgttcggg ctgctgggtt tggcccccgg ccccgatatc
     9001 ggcctggccg ccgcggccgc cctcaccggc ctgccgccgg cccggacccg atccgttctc
     9061 cgcgccctgc aacgggtttc gctgctgcac caggacgtgc cggatcgctg gcggctgcac
     9121 gacctgatcg ccctgtacgc cgccgaccag gccggctccg atgacgtggc gctgcgccgt
     9181 ctggtcgact cctgtgtccg caccgcctgc ggcggcgacc acctgctcga cccgcatcgg
     9241 cagccgatcg aggttgagcc tcttctgttg ccggacaagg actccgcact ggcatggttc
     9301 accgccgagc acgcctgtct gctcgccgct ctgcaattgg cggtccgccg cggctggcat
     9361 ctggaggtgt ggcggctggc ctgggcgttg acgaccttcc atcgccgaca ggggcatctg
     9421 tcggtgaatc tggactgctg gctgctcgcg ctggacgccg ccgaccaggt cggcgatccc
     9481 gggctgcggc agcgcaccca ccggctgctc gggcgggcct tcgcccgggt cggctcgaac
     9541 gcccgggcgc tgttccatct ccggcaatca ctggaactcg ccgaggacgt gccggagcgg
     9601 gcccattccc acatcgcgct ggcgcgcgcc ttggcacggg ccggcgacga cgagcaggcc
     9661 ctcagccacg ccaccgacgc ccttgcgctg taccgggaac tagccgcgcc ggcgtgggaa
     9721 gccactgccc tcaacgagat cggcctgatc agcgcccggg tcggcaagct cgacgacgcc
     9781 cgtctcgcct gcgaggccgc gctgctactg caccgtgaac agggcaatcg ggacggagag
     9841 gccaacacac tcgacaacct cggccgcctc ggctccgacg tggcggcggt gcggcactat
     9901 gagcaggcgt tggcgctgta ccgggagctc ggcaacaccc acgacgaggc cgacacactc
     9961 gaccgattgg ccgaacggca cctgggcctc ggcgaccgcg cacgggcctc agcacggtgg
    10021 cgggaggcgt tggccttgta ccaggcccag ttccgcgacg ccgacagtga ccgcgtgcga
    10081 cgccgactcg gatcgttcgg acgataaacc gtattcgcac ggacgatgac ggcggcgttt
    10141 ttccggggca gcatggccgc atgtactccc tcgacatcga cggcgccgac atccccggcg
    10201 agggcgcccg gctgcgggac gccggttccg ccgtgccggt ggaacttccc ggcggcgtgc
    10261 gggtctgggc cgtgccgcac ctggagacgc tgaagtcgct gctgctggac cgtcgggtgt
    10321 cgaaggatcc cgagctgcac tgggccgcgt ggcgggccgg cgagatcgcc gccgactggc
    10381 cgctgcggat ctgggtgtcg gt
//
