LOCUS       NZ_CP073318            10975 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Kutzneria sp. CA-103260 chromosome, complete genome.
ACCESSION   NZ_CP073318
VERSION     NZ_CP073318.1
KEYWORDS    RefSeq.
SOURCE      Kutzneria sp. CA-103260
  ORGANISM  Kutzneria sp. CA-103260
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kutzneria.
REFERENCE   1  (bases 1 to 11609901)
  AUTHORS   Jorgensen,T.S., Gren,T., Kontou,E.E., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Complete Genome Sequence of the Rare Actinobacterium Kutzneria sp.
            Strain CA-103260
  JOURNAL   Microbiol Resour Announc 10 (30), e0049921 (2021)
   PUBMED   34323613
REFERENCE   2  (bases 1 to 11609901)
  AUTHORS   Joergensen,T.S., Gren,T., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2021) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-APR-2021
            Assembly Method :: Flye v. 2.8-b1674; unicycler_polish v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:18:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,326
            Genes (coding)                    :: 10,257
            CDSs (with protein)               :: 10,257
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 9 of 69
            Pseudo Genes (incomplete)         :: 63 of 69
            Pseudo Genes (internal stop)      :: 2 of 69
            Pseudo Genes (multiple problems)  :: 5 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:59:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2064965
            Orig. end                         :: 2075940
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073318.1.
            Bacteria and source DNA available from Professor Tilmann Weber,
            Technical University of Denmark, Kemitorvet Building 220, room 327D
            2800 Kgs. Lyngby, Denmark.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10975
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10975
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10975
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5975](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5975)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            285..1472
                     /locus_tag="JJ691_RS09130"
                     /old_locus_tag="JJ691_18240"
     CDS             285..1472
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /locus_tag="JJ691_RS09130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18240"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_211768338.1"
                     /transl_table=11
                     /translation="MSRYRLLPTAAQETGLVEHCALARYVWNLAVEQHSHWQPGRAWAP
                     NYIEQARQLTEARAEFYWLRAGSQTVQQQALRDFAQAMRNFFGGTHRRPTWRKAGRHEG
                     FRQVGVRPEHVRKLNRRTGRVWAPKVGWVQFRWTRAVAAGVKSYRVTRDRAGRWHVAFA
                     VVPEPIAAPSTGEIVGVDRGVAVSAALSTGEFLHCPGLSEREQRRLRKLQRCLARARRG
                     SNRRGRVKAAIAKLKARAVDRRKDWVEKTSTDLARRFDLIRVEDLNIRGMTGSARGTVV
                     RPGRNVRQKAGLNRGILAAGWGRLVARLEHKAPGRVEKINPAFTSQTCSVCGHCASENR
                     ESQAVFRCVACGHQANADVNAARNIAAGRAVTARGGRALAGPANREPQLGPPSAA"
     gene            complement(1521..3572)
                     /locus_tag="JJ691_RS09135"
                     /old_locus_tag="JJ691_18250"
     CDS             complement(1521..3572)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /locus_tag="JJ691_RS09135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18250"
                     /product="MMPL family transporter"
                     /protein_id="WP_211768339.1"
                     /transl_table=11
                     /translation="MSGGTTLLNRVADFAVTRPRVVLVGAAVFLLLSVVCGYGVADQLR
                     GLGATDPSAPSSVAARTLSADFPGSAPNLVLLVDCDRGVDAPACVADGSAVAGRLAGEA
                     SVTGVTSYWQGRSATLRSRDGRQALITARILGSELAAADQSARLAAAYDGRHGSVLVRT
                     GGQAAVADQARRAIADDLARAELLSLPLTLAVLVLVFGSAVTALLPLAVGAIAMVGTGA
                     VLRAVSAFADVSVFAQELSVALGLGLAIDYALFIVRRFREERPHPDAVRRAVLTAGRTV
                     VYSAATVAVSLGALFLFPLGFLRSFAYVGVSVVLLAAVAALTVVPAALTVLGDRVDAGR
                     LVRSSDVDGRGWTRVVIRFAPLVAILVALPLVLAALPFRDVRFGSIDDRQLSADAPARV
                     VQQEIRDHFSGDAAGRINVLTSAPAAAVAARLATLPSVVAVTPLGRVGDTSVLAVESAD
                     DAVSPASQDLVRAIRAQHKDFPLEVAGPTAELVDAQAAVGSRLPLALACIAAATLLLVF
                     LMTGTVLLPVKTLLLNALSLTATFGAVVWVFQDGHLSGPLGFTPVGAIDTTLPVLMFCL
                     AFGLSMDYGLLVQSRIQEEYARTGDGREAVAVGLDRGARVVTAAAAVLVIVLVAVGTSS
                     VLNMKMLGLGSALAIAVDATVVRCLLVPAVMVLFGRITWWSPFTREKR"
     gene            complement(3572..4402)
                     /locus_tag="JJ691_RS09140"
                     /old_locus_tag="JJ691_18260"
     CDS             complement(3572..4402)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JJ691_RS09140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JJ691_18260"
                     /product="hypothetical protein"
                     /protein_id="WP_211768340.1"
                     /transl_table=11
                     /translation="MSASRLWSFVLVRFRPEVYVSYGVLWSLALEAVASDHWRPSPATV
                     ARAASVVLTLLFLRMLDEQKDLDYDRRHHPDRPLVTGAITSSELRSAMMVCTLCVLALN
                     SLLSVVSLVLATLPLGYGLALAAAERRFPAVRDGVLVSLAASYPVQLLVSCYLYVSSTG
                     AVGWRAVPVVLVFAFAFLHFEFARKTRWLGMAGERLYSLALGPALSGTVTLCWAVAAVV
                     LIVSLTTTWFVVLLVPPVLAARRFLAGTSESWPLPAAMAFVLLSYLSLIAYGLR"
     gene            complement(4405..5007)
                     /locus_tag="JJ691_RS09145"
                     /old_locus_tag="JJ691_18270"
     CDS             complement(4405..5007)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332641.1"
                     /locus_tag="JJ691_RS09145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18270"
                     /product="nitroreductase family protein"
                     /protein_id="WP_211768341.1"
                     /sec_met_domain="PF00881 (E-value: 3.8e-21, bitscore: 75.1,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIDEALTTTRAVRRGIDLRRPVTRGVIEQCLRIAVQAPTAANRQ
                     DWRFVGIDDPGLKHTIADYYRAASTAYLERAPNTADTESARFLAQHIDRMPVLLLPCVN
                     GRHSPSEHPARTASRYGSLYPAVWSFMLAARSRGLATAFTTVHLAYEREVADLLGIPYE
                     EVTQGCLVPVGYARRWFGPAPRRPVTEVTNWNRWRHG"
     gene            complement(5001..5975)
                     /locus_tag="JJ691_RS09150"
                     /old_locus_tag="JJ691_18280"
     CDS             complement(5001..5975)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="JJ691_RS09150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18280"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_211768342.1"
                     /sec_met_domain="AfsA (E-value: 9.4e-21, bitscore: 72.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQLTFSRTVPRELVHRSSVAEVFVTSLVEHGGQIEVGVQLPRSH
                     AFFGDTDHGHHDPLAFTEAARQACLAVAHQCFDVPAEARFVLRGFELRVLDPAGIAIGD
                     TPCDAVLHCHVVRRFTRDSRLHGLQLRHRAEIGGRHVLTADFAFSWLPENEWAAIRGTI
                     EPAPAARPQPRCAASVVGRACARNVVIGPPHVEGSFARAAVVVDTSHPTLFDHALDHTP
                     GMVLVEASRQLATACASVSANLSAGLVTGLRCRFTEFTELGAETTCHCQLGSVQPAGAG
                     REVHAWCWLNQSGRSVAEAQLRLSFPDTAVAGPHVDCQAERTC"
     gene            complement(5963..6838)
                     /locus_tag="JJ691_RS09155"
                     /old_locus_tag="JJ691_18290"
     CDS             complement(5963..6838)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /locus_tag="JJ691_RS09155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18290"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_211768343.1"
                     /transl_table=11
                     /translation="MTDVYLDDCAAQLDRLATDPATFGRDHGLLLLKPDAVVARRLLPA
                     VDWLSHHGFRIVAAERVSLDRHAIRAVWREPWRAATWQRRRLADLLATAADSLVLLVRA
                     ENAGPTPVCVRLTEMKGDGDPRRQRPGQLRHALGSPGFLLNMAHTADEPADVLRELAIY
                     FGDDDLQRVLRGALTGVDQSMRAKQIAAELHACFPSQCLDLAERAATLLGEVDNLLAGD
                     VVTAVARECLRDAVGPRNADLRAALAGLLLWAWQQGVDLDPWNVIVVGAAVLPMSNQQT
                     ETRLGAACTS"
     gene            complement(6835..7788)
                     /locus_tag="JJ691_RS09160"
                     /old_locus_tag="JJ691_18300"
     CDS             complement(6835..7788)
                     /NRPS_PKS="Domain: NAD_binding_4 (41-202). E-value:
                     9.7e-17. Score: 52.8. Matches aSDomain:
                     nrpspksdomains_JJ691_RS09160_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /locus_tag="JJ691_RS09160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18300"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_211768344.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.3e-11, bitscore:
                     41.1, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.7e-14, bitscore:
                     52.8, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVIVTGAAGLVGAEVCVRLAVAGHNVLGLTHHRKPPFPTTPSVQ
                     WFAADVTRPDLGLPPRSVDRLRADRCVLVHAAAVTAFGRDDSVYDAVNVGGTAHALALA
                     GRGEMPFVHISTAYVDQPYANGYERSKAAAESLVHGSGVDAVVIRPSIVVGAEATGAIR
                     LFRHMYVLLRLVLEGKVGVLPGRYDACLDLVPVDRVADLVTDVVQRFGEVRNSTLPAIA
                     GSPVTLRECSEVFAEYPSFRVPRFVPPDVFRPDALPDTQRRYHDRVVRLYEPYLRSRTV
                     FDDSATRAFHGRRPELTGVAHLRRLLDYCLAMGYLR"
     aSDomain        complement(7183..7665)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJ691_RS09160_NAD_binding_4.1"
                     /evalue="9.70E-17"
                     /label="JJ691_RS09160_NAD_binding_4.1"
                     /locus_tag="JJ691_RS09160"
                     /protein_end="202"
                     /protein_start="41"
                     /score="52.8"
                     /tool="antismash"
                     /translation="PSVQWFAADVTRPDLGLPPRSVDRLRADRCVLVHAAAVTAFGRDD
                     SVYDAVNVGGTAHALALAGRGEMPFVHISTAYVDQPYANGYERSKAAAESLVHGSGVDA
                     VVIRPSIVVGAEATGAIRLFRHMYVLLRLVLEGKVGVLPGRYDACLDLVPVDRVADL"
     gene            complement(7785..9293)
                     /locus_tag="JJ691_RS09165"
                     /old_locus_tag="JJ691_18310"
     CDS             complement(7785..9293)
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /locus_tag="JJ691_RS09165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_18310"
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_211768345.1"
                     /transl_table=11
                     /translation="MTDLDFDQLLKMALAEIDAPPTSAVVLEGSIAEGFGTPSSDIDFL
                     VLVDGEDQPGIPAVLFLGGRRVEVRMRSLRQVVEEFDTAFTVPEPDVLDRCQRLLGAVP
                     LRGSDMLHRIRDAVSAEAFADQVSDWFADRSRHALRFAQAMAELEQPDEADDWARFGLL
                     QSAKSWAAHRGETYLEPKWLSAQLDRIEARAGSPQDLVRRYREGVAGTAACVELAEDFG
                     VTGCRPEPGGVRLRRIPQVTTWPIGDRVHVLRDGADVFTLDEPAGRVWQSIVFDRPPAD
                     GVDRGTLALFVRLGLVGLSWPDGTAIRPAAPPTTPPPGRASPALTALGGTVVGPVTLVP
                     LPADRFCAAGLDLVWSNVLAENAREDLLGALAVGSDETAGFAARRMAQVACRALFSAAG
                     IHPLPPDDTLTRSMAELPFVDGAVVALARRAERPPVSLAKLDHLVAAVRGYAGATGFPA
                     SFDSAAEWQRTIELGHDWLRLAAHLDADLPIRDAADLFTPRRTA"
     misc_feature    9262..9277
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.83"
                     /tool="antismash"
     misc_feature    complement(9372..9385)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
ORIGIN
        1 atgggtcagt tcgggcgtgg tcaccacgta caccggcagc cgacccaggt ggatccggac
       61 gacatcgccg cgcccgcgaa gggaagtgac gaaccgcagt ggccggcgca gcagcggcag
      121 gccgtggccg accagcggca gccggtgggg catggtggtg acggtgttca tcaagtctcc
      181 aatcggtgga gaccccggag ttcaggccgg ggaggagccg atccgaccgc ccgcggccgc
      241 gtcggcggcc gggattgtcg gtggccccga ctacggtgat cgtcgtgtcc cgctatcgcc
      301 tgctgcccac ggccgcccag gagacagggc tggtggagca ctgcgcgctc gcgcggtatg
      361 tgtggaacct cgccgttgaa cagcactctc actggcagcc cggacgcgcc tgggccccga
      421 actacatcga gcaggcccgc cagttgaccg aagcgcgtgc cgagttctat tggttgcgcg
      481 cgggcagcca gacggtgcag cagcaggcgt tgcgggactt cgcccaagcc atgcggaatt
      541 tcttcggtgg cactcaccgc aggcccacat ggcgtaaggc cgggcggcat gaggggttcc
      601 ggcaggtcgg cgtgcggcct gagcatgtcc gcaagctcaa ccgccggact ggtcgggtgt
      661 gggcgcccaa ggttggctgg gtgcagttcc gctggacccg tgctgttgcg gccggggtga
      721 agtcctaccg ggtcacccgc gaccgggccg gtcgctggca tgtcgccttc gctgtggtcc
      781 ctgagccgat cgcggcgccg agcaccggtg agatcgtcgg cgtggaccgc ggagtggccg
      841 tgtctgcggc gttgtccacg ggagagttcc tgcactgccc tggtctgtcc gaacgggagc
      901 aacgccggtt gcgtaagttg cagcggtgcc tggcccgcgc gcggcggggt tcgaatcggc
      961 gcggtcgggt gaaggccgcg atcgcgaagc tgaaggccag ggccgtggac cggcgtaagg
     1021 actgggtcga gaaaaccagc accgacctgg cccgccggtt cgacctgatc cgcgtcgagg
     1081 atctcaacat ccggggcatg actggctcgg ccaggggcac cgtcgtgcgg cccggccgga
     1141 atgtgcgtca gaaggccggg ttgaaccggg gcatcctcgc cgctgggtgg ggccggctgg
     1201 tggcccgctt ggagcacaag gcgccgggcc gggtcgaaaa gatcaatccc gccttcacgt
     1261 cgcagacctg ctcggtctgt gggcactgcg cgtcggaaaa ccgcgagagc caagcggtct
     1321 tccgatgcgt ggcctgcgga caccaggcca acgccgatgt gaacgcggcc aggaacatcg
     1381 cggcagggcg tgccgtgacc gcgcggggag gccgggcgtt ggccgggccg gcgaaccgtg
     1441 aacctcaact cggacctcct tccgccgcgt agcgggactg atacgggttg gaatccccgt
     1501 cgttcacgac ggggaggacg tcaacgtttc tccctggtga acgggctcca ccacgtaatc
     1561 cgtccgaaca ggaccatcac ggcggggacg agcagacagc ggaccacggt ggcgtcgacg
     1621 gcgatcgcga gggcggatcc caggcccagc atcttcatgt tgagcacgct cgacgtgccg
     1681 accgcgacga gcacgatgac gaggacggcc gccgccgcgg tgacgacccg ggcgccgcgg
     1741 tccagcccga cggccacggc ttcccggccg tcgccggtcc gcgcgtactc ctcctggatc
     1801 cgggactgca cgagcaggcc gtagtccatg gacagcccga acgccaggca gaacatgagc
     1861 accgggagcg tggtgtcgat ggcgccgacc ggggtgaacc cgagtggacc tgacaggtga
     1921 ccgtcctgga acacccacac caccgcgccg aacgtcgcgg tgagactgag cgcgttgagc
     1981 agcagggtct tgaccgggag caggacggtc ccggtcatca ggaacaccag cagcagggtc
     2041 gcggcggcga tgcacgccag cgcgaggggg aggcggctgc ccacggcggc ctgggcgtcg
     2101 acgagctccg cggtcgggcc ggcgacttcc aacgggaagt ccttgtgctg ggcgcgaatc
     2161 gcgcggacga gatcctggct ggccggcgac acggcgtcgt cggccgactc caccgcgagg
     2221 acgctggtgt cgccgacccg gccgagcggg gtcacggcga ccaccgaggg cagggtggcc
     2281 aggcgagctg ccaccgccgc cgcgggcgcc gaggtgagaa cgttgatccg cccggcggcg
     2341 tcgccgctga agtggtcgcg gatctcctgc tgcaccacgc gggcgggcgc gtcggccgac
     2401 agttgccggt cgtcgatcga gccgaagcgg acgtcgcgga acggcagcgc cgccagcacc
     2461 agcggcagcg ccacgaggat ggcgaccagg ggtgcgaacc tgatgaccac acgcgtccac
     2521 ccgcgaccat ccacatcgga cgatcggacc agacggccgg cgtcgacgcg gtcgccgagc
     2581 accgtcagcg cggccggcac gaccgtgagc gcggcgaccg cggcgagcag cacgacgctc
     2641 acgccgacgt acgcgaacga gcgcaggaaa cccaggggaa acaggaagag ggcgccgagc
     2701 gacacggcga cggtggcggc ggagtacacg accgtgcggc cggccgtcag cacggcccgt
     2761 cggaccgcgt cgggatgcgg ccgctcctcg cggaaccgtc gcacgatgaa cagcgcgtag
     2821 tcgatggcca acccgaggcc gagcgcgacg ctgagctcct gggcgaagac cgacacatcg
     2881 gcgaacgcgc tgacggcccg cagcacggcg cccgtgccca ccatggcgat cgcgccgacc
     2941 gcgagcggca gcagcgccgt gaccgcactg ccgaacacca ggacgaggac cgccagcgtc
     3001 agcggcagcg aaagcagttc cgcgcgggcc aggtcgtcgg cgatcgcgcg ccgtgcttgg
     3061 tcggccaccg cggcctggcc gccggtccgc accagcacgc tgccgtgacg gccgtcgtag
     3121 gcggcggcga gccgggcgga ctggtcggcc gcggccagtt cggagccgag gatccgggcg
     3181 gtgatcaacg cctgccggcc gtcgcgtgag cgcagcgtcg ccgagcggcc ctgccagtac
     3241 gacgtgacac cggtgacgga cgcctcgccg gccagccggc ccgcgacggc ggagccgtcg
     3301 gcgacgcagg ccggcgcgtc cacaccgcgg tcgcagtcca cgagcaggac gagattgggg
     3361 gcgctgccgg gaaagtccgc cgacaacgtg cgggctgcca ccgacgaggg cgctgacgga
     3421 tccgtcgcgc cgagaccacg caactggtcg gccacgccat agccgcagac gacggacagg
     3481 agcaggaaca cggccgcgcc gacgaggacg acgcggggac gcgtgacggc gaagtcggcg
     3541 acgcggttga gcaaggtggt gccgccggac atcagcgcag cccgtaagcg atgagggaca
     3601 ggtagctcag cagcacgaag gccatggcgg ccggcagcgg ccaggactcg ctggtgccgg
     3661 cgaggaaccg ccgggccgcg aggacgggcg gcaccaacag cacgacgaac catgtcgtgg
     3721 tgagcgacac gatgagcaca accgcggcga cggcccagca gagcgtcacg gtgccactca
     3781 gcgccgggcc gagtgccagc gagtacagcc gctccccggc catgccgagc caccgcgtct
     3841 tgcgggcgaa ttcgaagtgc aggaacgcaa aggcgaagac gagcaccacc ggcaccgcac
     3901 gccagccgac cgcgccggtc gacgacacgt agaggtagca gctgaccagc agctgcaccg
     3961 ggtagctggc cgcgaggctc accaggacac cgtcgcgcac ggccgggaac cggcgctccg
     4021 ccgcggcgag ggcgagtccg tagccgaggg gcagcgtggc caatacgagc gacaccaccg
     4081 acagcaggga gttgagcgcc agcacgcaca gcgtgcacac catcatcgcc gagcgcagct
     4141 ccgaagaggt gatcgccccc gtcaccagcg gtcggtccgg gtggtgccgg cggtcgtagt
     4201 cgagatcctt ctgctcgtcg agcatccgca ggaacagcaa cgtcagcaca acgctcgcgg
     4261 cgcgggcaac cgtggccggt gacggtctcc agtggtcgct cgccaccgcc tccagggcga
     4321 gcgaccacag cacgccgtag ctgacgtaca cctcggggcg gaacctgacc agcacgaagc
     4381 tccacaaccg cgacgcggac acgatcatcc gtgccgccag cggttccagt tggtcacctc
     4441 ggtgaccggc cgccggggcg ccgggccgaa ccagcgcctg gcgtagccga ccggcaccag
     4501 acacccctgg gtgacctcct cgtacgggat gccgagcaga tcggcgacct cccgctcgta
     4561 ggccaggtgc acggtggtga aggcggtcgc gaggcccctg gaccgtgcgg cgagcatgaa
     4621 gctccagacc gccgggtaca gcgagccgta gcggctggcg gtccgtgccg ggtgctcact
     4681 gggggagtgc cggccgttga cgcacgggag cagcagcacc ggcatgcggt cgatgtgctg
     4741 ggccaggaac cgggcggatt cggtgtccgc ggtgttcggc gcccgctcca ggtacgccgt
     4801 cgaggccgcg cggtagtagt cggcgatcgt gtgcttcagc ccggggtcgt cgattccgac
     4861 gaaccgccag tcctgtcggt tcgcggcggt cggcgcctgc accgcgatcc gcaggcactg
     4921 ctcgatgacg ccacgcgtga ccggccggcg caggtcgatg ccgcgacgca cggcccgcgt
     4981 ggtggtgagc gcctcgtcga tcagcatgtg cgctccgcct ggcagtcgac gtgcggcccg
     5041 gccaccgccg tgtccgggaa gctcagccgc agctgcgcct cggcgaccga gcgaccggac
     5101 tggttgagcc agcaccaggc atgcacctca cggccggcgc ccgccggttg gaccgagccc
     5161 agctggcagt ggcacgtggt ctcggcgccg agttcggtga attcggtgaa ccggcagcgc
     5221 agcccggtca ccaggccggc gctcaggttc gcggacaccg aggcgcacgc cgtcgccagc
     5281 tgccgggacg cctcgaccag caccattccc ggagtgtggt cgagcgcgtg gtcgaacagg
     5341 gtcggatggg aggtgtccac gacgacggcg gcgcgggcga agctgccctc gacgtgcggg
     5401 ggaccgatca ccacgttgcg ggcgcacgcc cgcccgacga cggaagccgc gcacctgggc
     5461 tgtggccgcg ccgccggcgc cggctcgatg gtgccgcgta tcgcggccca ctcgttctcg
     5521 ggcagccagg agaaggcgaa gtcggcggtc agcacgtgcc ggccgccgat ctcggcccgg
     5581 tgccggagtt gcaggccgtg caggcgggag tcccgcgtga accgtcgaac cacgtggcag
     5641 tgcaggacag cgtcgcacgg ggtgtcgccg atggcgatgc cggccgggtc gagcacccgg
     5701 agctcgaacc cgcgcagcac gaaccgcgct tcggcgggca cgtcgaagca ctggtgcgcc
     5761 accgcgaggc acgcctggcg ggccgcctcc gtgaacgcca gcgggtcgtg gtggccgtgg
     5821 tcggtgtccc cgaagaacgc gtgcgaccgg ggcagctgga cgccgacctc gatctggccg
     5881 ccgtgctcga cgagcgacgt gacgaacact tcggcgaccg acgaccggtg caccagctca
     5941 cggggcacgg ttcgggagaa cgtcagctgg tgcatgccgc ccctagtctc gtttcggtct
     6001 gctggttgct catcggcagc accgcggcgc cgacgacgat cacgttccac gggtcgagat
     6061 cgacgccctg ctgccaggcc cagagcagca gcccggcgag cgccgcgcgc aggtccgcgt
     6121 tccgcgggcc gaccgcgtcc cgcaggcatt cgcgggccac cgcggtcacg acgtcgccgg
     6181 cgagcaggtt gtcgacctcg cccaacagcg tggcggctcg ctcggccagg tccaggcact
     6241 gcgacgggaa gcacgcgtgc agctcggcgg cgatctgctt ggcgcgcatg gactggtcca
     6301 cgccggtcag cgcgccgcgc agcacccgct ggaggtcgtc gtcgccgaag tagatggcca
     6361 actcccgcag cacgtcggcg ggctcgtcgg ccgtgtgggc catgttgagc aggaagccgg
     6421 gcgagccgag cgcgtgccgc agctgacccg gccgttgccg gcgcggatcg ccgtcgccct
     6481 tcatctcggt gagccggaca cataccggcg tcggcccggc gttctcggcc cgcaccagga
     6541 gcacgagcga gtcggccgcg gtcgccagca gatcggccag tcgccgccgc tgccacgtcg
     6601 cggcccgcca tggctcccgc cacacggcgc ggatggcgtg ccggtccagg ctgactcgct
     6661 cggccgcgac gatccggaag ccgtggtgtg acaaccagtc gacggccggc agcagccgtc
     6721 gcgccaccac ggcgtccggc ttgagcagga gcaggccgtg gtcgcggccg aacgtcgccg
     6781 ggtccgtcgc gaggcggtcc agttgcgccg cacagtcatc caggtagacg tcggtcatcg
     6841 gaggtagccc atcgccaggc agtagtccag cagccgccgc aggtgcgcca cgccggtcag
     6901 ttcgggccgg cggccgtgaa acgcccgggt cgcgctgtcg tcgaagacgg tccggctgcg
     6961 caggtacggc tcgtacagcc ggaccacgcg atcgtggtag cggcgctggg tgtcgggcag
     7021 cgcgtccggc cggaacacgt ccggcggcac gaatcgcggc acccggaacg aggggtactc
     7081 ggcgaacacc tccgagcact cccgcagcgt gacgggactc ccggcgatcg ccggcagcgt
     7141 gctgttgcgc acctcgccga accgctgcac gacgtccgtg acgaggtccg cgacccggtc
     7201 gaccgggacc aggtcgaggc acgcgtcgta gcggccggga agcacaccga ccttgccctc
     7261 caggaccagc cgcagcagca cgtacatgtg gcggaacagg cggatcgcgc cagtggcctc
     7321 cgcgccgacc acgatgctcg gccggatcac caccgcgtcg acgccgctgc cgtggacgag
     7381 gctctcggcc gctgctttgc tgcgctcgta gccgttcgcg tacggctggt cgacgtacgc
     7441 ggtgctgatg tgcacgaacg gcatctcacc gcggccggcc agtgccagcg cgtgcgccgt
     7501 gccccccacg ttgaccgcgt cgtagacgct gtcgtcgcgg ccgaaggccg tgacggccgc
     7561 cgcgtgcacc aggacgcacc ggtcggcgcg cagccggtcg accgatctcg ggggcagccc
     7621 gagatcgggc cgggtgacgt cggcggcgaa ccactggacg gacggtgtcg tcgggaacgg
     7681 gggcttgcgg tggtgggtca ggccgagcac gttgtgcccg gccacggcga gccggacgca
     7741 gacctcggcc ccgaccaggc cggcggcgcc ggtgacgatc acgttcatgc cgtccgcctc
     7801 ggggtgaaca ggtccgcggc gtcgcggatg ggcaggtcag cgtcgaggtg ggcggccagt
     7861 cgcaaccagt cgtggccgag ctcgatcgtg cgctgccact ccgcggcgga gtcgaaggag
     7921 gccgggaagc cggtcgcgcc ggcgtacccg cgcaccgccg ccacgagatg gtccagcttg
     7981 gcgagcgaga ccggcggccg ctcggcccgc cgggccagtg ccaccaccgc accgtcgacg
     8041 aacggcagtt cggccatcga gcgggtcagc gtgtcgtccg gcggcagcgg gtgtatgccg
     8101 gcggcgctga acagcgcgcg gcacgccacc tgcgccatcc gacgcgcggc gaacccggcg
     8161 gtctcatcgc tgccgacggc cagcgcgccg agcaggtctt cccgggcgtt ctccgccagc
     8221 acgttggacc acaccaggtc gaggccggcg gcgcagaacc ggtccgccgg cagcggcacg
     8281 agcgtcaccg gcccgacgac cgtgcccccc agcgcggtca gcgccgggct ggcccgaccg
     8341 ggcggcggcg tggtgggggg cgcggccggc ctgatggccg tcccgtcggg ccaggaaagg
     8401 ccgacgaggc cgagccggac gaagagcgcc agcgtccccc ggtcgacgcc gtcggcgggc
     8461 ggccggtcga acacgatcga ctgccacacc cggccggccg gctcgtccag cgtgaacacg
     8521 tcggcgccgt cgcggagcac gtgcacccgg tccccgatcg gccaggtggt cacctgcggg
     8581 atccggcgca gccgcacgcc gcccggttcg ggccgacacc ccgtgacgcc gaaatcctcg
     8641 gccagctcga cgcaggccgc cgtgccggcg acgccctcgc ggtagcggcg cacgagatcc
     8701 tgggggctgc cggcccgcgc ctcgatccgg tcgagctgag ccgacagcca cttcggctcc
     8761 aggtaggtct cgccgcggtg cgcggcccac gacttcgcgc tctgcagcag gccgaacctg
     8821 gcccagtcgt cggcctcgtc cggctgttcg agttcggcca tggcctgtgc gaatcgcaac
     8881 gcgtgcctgg accggtcggc gaaccagtcc gacacctggt cggcgaacgc ctcggccgac
     8941 accgcgtctc tgatgcggtg cagcatgtcc gagccccgca gcggcacagc gccgagcagc
     9001 cgttggcacc ggtcgaggac gtccggctcg ggcaccgtga acgccgtgtc gaactcctcg
     9061 accacctggc gcagggaccg catgcgcacc tcgacccgac ggccgccgag gaacagcacg
     9121 gcgggaatgc ccggctggtc ctcgccgtcc acgagcacga ggaagtcgat gtcggaggag
     9181 ggggtgccaa acccctcggc gatcgagccc tccagcacga ccgcgctcgt cggcggcgcg
     9241 tcgatctccg ccagcgccat cttgagcagc tggtcgaaat ccaggtccgt cacggttcga
     9301 gcacctccac cagaccggcg ccgccgtcga cccgcagccg tgcgccggcg cggacactgg
     9361 cgagcaggcc gttgatctgc accacggtcg gaatgccgag ttcgcgggcg acgacggcga
     9421 cgtgcgtgag cgggctgccg cgttcgacca gtagggcgga ggcgccggtc agcgcggcga
     9481 cggaacccgg atccgtgtgg cgcgccagca ggatgccggt cccaccgtca ccggcgcgca
     9541 cggccgtgcc ctccaccacg cccgggcttg acggcgtgcc acgcaggacg cgtgctggcg
     9601 tgtccgcggt ccgggcgggc gcaccccagc ccgcgcgttg gagattgccc agccagtacg
     9661 gcgcgccgcg agtctcgaag cgcgcgggcg cggtgacgcg ctcgtcccgg agccgctgct
     9721 cgcggcgcag ccacacgacc ggtttgagtt cgacgtgcgc ggtggttccc tcgcagcacc
     9781 cgcgcacctc gtccaggcgc agcaggaata cgtcctgcca gtcgtcgagc gcgccgagtc
     9841 gggccaggtc cttcccgatc gcccgcagca tccgcttggc catgccgaac gccttcgcgc
     9901 gccgccagcg cagccgctcc cgatcggcca tgccggcgct gaccttgcgg cggacgacac
     9961 cgtaggcgat tcggcgcacg ctgcccaggt gcgcgtcgag gtaaccgtcc acatcgccgc
    10021 gggtggcgtc caaggtggcc ggcggctccc gcagcatcgt gaacagtgct gagggatcct
    10081 cggtcagatc cggtgtctcc agcttcatct cgtcgacgcc gcggaacccg aaccgcgcga
    10141 tgtagtcgtc caccgcgtcg aggaacccgt gccggccggt cgcggccagc cgcgcccgca
    10201 cctggtcggc cggcgtgtcc cggaccagcg cggccaactc gggatccccg tgcagttcca
    10261 gggccagtcg gcgcaggacg gtcaccggtt cggtggattc caccgccggc ccgggaccgg
    10321 cggccgccca gaagaaccag tcgggtgcgg ccggcaacca ccggcgggtc aacaggtgca
    10381 gcacgccgac cgaggtcagc agcgacgcgt ccaggaccat gaccggcccc cagcgctcga
    10441 tgagttcccg ctggagcagc cgaaaccggc cgtacacctc gtcgcagggc atgccgtcgt
    10501 agtcgactcg gtcgaattcc gttgcggcgg cgtcgaattc gcggcagaac cggcgaaccg
    10561 tgcggccgat ggccagcgac cgcacggcga acacggcgcc ggtggccagc gccgccagcg
    10621 gagaccgccg gtacgggcgg agtccgtcgg cgatctcatc gtcgagcggc ggcacgccga
    10681 gggagacctc gaacaccttg cggttgagcc ggtatccggg cgccagccgt accatccgat
    10741 accagtgctg caagttgtag tagacacggc cgtggaagta tccgagcagg gccggcagcc
    10801 agtcggccag ttggcgccgc gcgccgcgag gaacccgcaa cgcgcgtgcg tattcctggt
    10861 acacccgcgc gtacacgtcc gccgcgaagg tgtacgtcag tggcgaggtg acgccactga
    10921 agctctcgac gatgttggag ttgtcccaga tccggcgttc gccgccgagc cgcgt
//
