LOCUS       NZ_CP073318            11146 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Kutzneria sp. CA-103260 chromosome, complete genome.
ACCESSION   NZ_CP073318
VERSION     NZ_CP073318.1
KEYWORDS    RefSeq.
SOURCE      Kutzneria sp. CA-103260
  ORGANISM  Kutzneria sp. CA-103260
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kutzneria.
REFERENCE   1  (bases 1 to 11609901)
  AUTHORS   Jorgensen,T.S., Gren,T., Kontou,E.E., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Complete Genome Sequence of the Rare Actinobacterium Kutzneria sp.
            Strain CA-103260
  JOURNAL   Microbiol Resour Announc 10 (30), e0049921 (2021)
   PUBMED   34323613
REFERENCE   2  (bases 1 to 11609901)
  AUTHORS   Joergensen,T.S., Gren,T., Gonzalez,I., Roman-Hurtado,F.,
            Oves-Costales,D., Thomsen,E., Charusanti,P., Genilloud,O. and
            Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2021) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-APR-2021
            Assembly Method :: Flye v. 2.8-b1674; unicycler_polish v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:18:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,326
            Genes (coding)                    :: 10,257
            CDSs (with protein)               :: 10,257
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 9 of 69
            Pseudo Genes (incomplete)         :: 63 of 69
            Pseudo Genes (internal stop)      :: 2 of 69
            Pseudo Genes (multiple problems)  :: 5 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:59:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1004021
            Orig. end                         :: 1015167
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073318.1.
            Bacteria and source DNA available from Professor Tilmann Weber,
            Technical University of Denmark, Kemitorvet Building 220, room 327D
            2800 Kgs. Lyngby, Denmark.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11146
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11146
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11146
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6146](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6146)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            4..1176
                     /locus_tag="JJ691_RS04375"
                     /old_locus_tag="JJ691_08750"
     CDS             4..1176
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /locus_tag="JJ691_RS04375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08750"
                     /product="DUF3089 domain-containing protein"
                     /protein_id="WP_211767514.1"
                     /transl_table=11
                     /translation="MLWGSLAAAVTLTASLVGPADALPAPVWLCHDSCGPTTATYPVSH
                     DQVPLDTTDVTGGPGVATGPGVDCFYVYPTVDTLPNLVPAASDEEFAQAIAQMGMLLPH
                     CRIFAPVYRQRTLPELALSMITKIPPDYSTGISDVEQAFQHYWDHDNTDPATGRRRGVV
                     LLGHSQGSAVLTALIQHKFDGNPEMTKQLVSAILLGGHIVVPTGRPDGGGSDPKATFQH
                     VPACQHATDTACVVAYSSFKPGGQTPGSDADIVRVAGDPSHQVLCVNPAALLTNAQGPV
                     PLDTVLYTRELVGGNDLNPYGRISGLGQSLTVPNYPTGFARYYGTLTGQCKQATDSNGT
                     VSWLDIVGGDNLVGPPQANFYGLHALDFNLAAGDLGTLIAKQAAAWSAGT"
     gene            1250..1528
                     /locus_tag="JJ691_RS04380"
                     /old_locus_tag="JJ691_08760"
     CDS             1250..1528
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JJ691_RS04380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JJ691_08760"
                     /product="hypothetical protein"
                     /protein_id="WP_211767515.1"
                     /transl_table=11
                     /translation="MGTKQDVVRQAESCWGISHAERHRADAVEITERAVAALRERAAAE
                     PDNADHEQKLSWALINLAQLHSARREHAKADAAALESVAILRRMLAR"
     gene            1525..2430
                     /locus_tag="JJ691_RS04385"
                     /old_locus_tag="JJ691_08770"
     CDS             1525..2430
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /locus_tag="JJ691_RS04385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08770"
                     /product="methyltransferase"
                     /protein_id="WP_211767516.1"
                     /transl_table=11
                     /translation="MTAALLGLHRAAATLSAYSAAVQLGLIDHIDRTPASVEDLAMTCG
                     ASVRGVRVVLATLADSGLVQRRADGRYEPATPGLAGVHPLLPWRDQVTDSVRTGLPARE
                     AERDVITGLLHGMRADVVTELPAARRALDVSVDGAPCGAALAARDPKCRVSLLDVPANR
                     RTDWSPQFDFLPGGLFDARPVAGEYDLVVLPGVCHLLDGAAAAELIRRLAPALRSGGTL
                     AVIDTLAGSQGAATHELSLLLRTRGGAIHDPADYRRWLAGAGPRRRGPAAGAHRADRAQ
                     ALVRLVHTLPRGVEAGWPAS"
     gene            2399..5035
                     /locus_tag="JJ691_RS04390"
                     /old_locus_tag="JJ691_08780"
     CDS             2399..5035
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /locus_tag="JJ691_RS04390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08780"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_211767517.1"
                     /transl_table=11
                     /translation="MESRLVGRHRELGIVDRAIDAVQGGDGRVLLVSGQAGIGKSRLAI
                     SALHKAKDHGFAVLMGSSSPLQAGLAYAPVVEALRRHLGSLPEPESARLLDGLHDIAGL
                     IADPRLSPAPLSGDPELERTRMFEAVLRLTGRITARRPALFVVDDLHWADRGTIELLHH
                     LSRGSRRRLLLWFGYRSAEPGGPLAALAMTVRRDDSSVEMALEPLSDNAVGELVGDLLG
                     TRPQDDVLRKVTARAQGVPLFVTALVGGGALRDAAGLPVIVRDVVLDRLQRLDDRARRL
                     LELIAVAGAGGSPRLLRAVWAEDGFDAVLRKLFDQGLIEELVDGPAQSFSALTYRVAHP
                     LYAEVAYAELTVGQRRSLHAAIAAELGPADVLEAAPHYRDAGDMVDPVRASEVLGAAGK
                     RALDVHDAHEAVNYLSVALNHAKAAGRADLVPDLMTDLAFALQASGRLDDAALMWDDAD
                     SIAQSAGDTLRQRLLGYYRSMLEAERGHPAELPRPVPFDDDDPPIGLMVHWLVAVRAGD
                     TATMWQALDQMINLARLRDDDLARSVAHFGAGMVARLGLDYAGAHTQNLIALETAELAP
                     PDVRDQYTYTPRLLAPSLALLRGDPAGGVRAADNPGAVSVQVFLPSAVHYVAYVRTFAL
                     FAAGDLPGALAVADESLALAERVVGQDRLVGRALAARAFLLVENGDLARAERDLKDARA
                     DYDTTYDDFVLAVDLAELAYALRTGAAVDDSAFLPHRPLRDLTVDWLRVAYSGYLAVAR
                     KNHAVAQQVLDDLRNGAKTSPFLDALAVRQEGLMLDDPERLDRAAARLAAMGAPLPVPK
                     PGTPPLSKREREIVRLVGAGLTNGQIAERLFLSERTIETHLHNSYKKLRLTTRPALTRW
                     ALEHAGD"
     gene            complement(5001..6146)
                     /locus_tag="JJ691_RS04395"
                     /old_locus_tag="JJ691_08790"
     CDS             complement(5001..6146)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="JJ691_RS04395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08790"
                     /product="YcaO-like family protein"
                     /protein_id="WP_211767518.1"
                     /sec_met_domain="YcaO (E-value: 1.9e-32, bitscore: 112.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVPAERGLPLDAALARGLAAAGELGLSPDFTPLLDANPGSWQCT
                     LRYGCVKVQAADGLGHGDPETAKVGALFEALEHYLCGLDGLRNRQVRLHDAHEVAHGPL
                     AADPLIAQLADGPARELGCVRYEALDGSADAHVPVFLTIPDYRGESGESARFTVEDFYD
                     YTAADPRIEAVGWAAGAYAAEALVHAVNETIERDALAQLLADRSRPLRVVDPAALPADL
                     AELLVTAADRSGRSVHLIDMTSDLDVPAFYAYQAPASGERPAAAGSAAALGSRVAAARA
                     LTELIQGQSAPVTRPIPDFTDRLPDAAEVGFDETKQPTTPREHLDLLVNMLRDKGFTPY
                     AREQYGTRNLAVVNVFVPGLERSTDGRRLRVSRRRAQAPTG"
     misc_feature    complement(6327..6341)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     misc_feature    6337..6352
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.88"
                     /tool="antismash"
     gene            6575..7393
                     /locus_tag="JJ691_RS04400"
                     /old_locus_tag="JJ691_08800"
     CDS             6575..7393
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /locus_tag="JJ691_RS04400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08800"
                     /product="M55 family metallopeptidase"
                     /protein_id="WP_211767519.1"
                     /transl_table=11
                     /translation="MRVLVSVDMEGIAGVVHSDDIQPGHREYERNRALLTAEANAAVRG
                     VYAYAADAQVLVADAHAQFRNLLPELLNQRAELLRGTPRPNGMLAGLDADIDAVLFVGY
                     HGKAGAARSVLAHTVSGRVIADVRCDGSSLGELGLNAALAAHYGVPSVLVSGDDTVAAE
                     AVEVAAGMRCVVVKRALGARAARMIHPEEACRRIELEVPLALADRAAVRARRFEGLVRL
                     EVQVHRPYMAEHALLVPGMERVDGCTLRYEAPDFPTAYNIVELIAVLGSL"
     gene            7581..8066
                     /locus_tag="JJ691_RS04405"
                     /old_locus_tag="JJ691_08810"
     CDS             7581..8066
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639276.1"
                     /locus_tag="JJ691_RS04405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08810"
                     /product="DUF5662 family protein"
                     /protein_id="WP_249227673.1"
                     /transl_table=11
                     /translation="MSYDSTPDTLWHSLRVAELMGELIKELLDRSVRHDLSKTREPERA
                     VYDEVVPQLRATTYGSVEYRTLVDAMGEGLRHHYAHNRHHPEHFPDGINGMTLVDLLEM
                     LADWKAATERASHGDLADSLTINRERFGIAPQLMDILANTARHFGWLATEPDRNAAP"
     gene            complement(8649..9263)
                     /locus_tag="JJ691_RS04410"
                     /old_locus_tag="JJ691_08820"
     CDS             complement(8649..9263)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646368.1"
                     /locus_tag="JJ691_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08820"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_211767520.1"
                     /transl_table=11
                     /translation="MTADKPLRADAQRKREALLATAREVFDAGDFFDLRFDDFARLAGV
                     GTGTLYRHFPTREALAEAVYHGEVATLCDRARQLQATLPAAEALAAFLRGMVDHMDAHQ
                     GLARTLATLMADRSSALAEGSRALEQAVTDLVAAAVKDGAVRDDVSAGAVMMALHGIGA
                     AHDRPGWRAEADGVITLVLDGLRRSYSGDSLREQNSAGELT"
     misc_feature    complement(9272..9285)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.75"
                     /tool="antismash"
     misc_feature    complement(9276..9291)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 20.61"
                     /tool="antismash"
     misc_feature    complement(9277..9291)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.75"
                     /tool="antismash"
     misc_feature    complement(9285..9299)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     misc_feature    complement(9298..9312)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.12"
                     /tool="antismash"
     misc_feature    complement(9312..9326)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.13"
                     /tool="antismash"
     gene            9354..10313
                     /locus_tag="JJ691_RS04415"
                     /old_locus_tag="JJ691_08830"
     CDS             9354..10313
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228220.1"
                     /locus_tag="JJ691_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJ691_08830"
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="WP_211767521.1"
                     /transl_table=11
                     /translation="MKILMFGRGVIATVYGWALQQAGHDVEFYVRPGRAAAYGDTVDLD
                     LFDMRRRVWGQRVVEKWPVRYREALEPDHDFDLIVLSVPHHRLAEAAAFLAPRVSKATV
                     LVFGNVWAEPLAAIGALPVDRIAWGFPQAGGGFGADGVLRGALLPSVVFGTLGQPPTDR
                     ERAVRQAFREAGFRLKERPDFRGWLWVHFVSDAGLFSQGLRLGSLSKLAGSTSDFREAL
                     LTGRELLPLLQARGIDLRRHRGGVLLFRVPTWLMAPALAWLAAHVAPVRVNFAAHSAPD
                     AEEPREVCRDTLAEARRLGVAVPRLEAAEPSFAREGKG"
     misc_feature    10483..10496
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.77"
                     /tool="antismash"
     misc_feature    10714..10728
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.56"
                     /tool="antismash"
ORIGIN
        1 cccatgctct ggggatccct cgcggccgcc gtcacgttga cggcgagcct ggtcgggccg
       61 gccgacgcgc tgccggcgcc ggtgtggctg tgccacgaca gctgtggccc gacgacggct
      121 acgtacccgg tcagccacga ccaagtaccc ctcgacacca cggacgtgac gggcggcccg
      181 ggcgtggcga cggggccggg cgtcgactgc ttctacgtct acccgacggt ggacaccctg
      241 ccgaacctgg tcccggcggc gtcggacgag gagttcgcgc aggccatcgc gcagatgggg
      301 atgctgctgc cgcactgccg gatcttcgcc ccggtgtacc gccagcggac gctgccggag
      361 ctggccctgt ccatgatcac caagatcccg ccggactact cgaccggcat cagcgacgtg
      421 gagcaggcct tccagcacta ctgggaccac gacaacacgg atccggcgac gggccggcgc
      481 aggggagtgg tgctgctggg ccactcgcag gggtcggcgg tgctgacggc gctgatccag
      541 cacaagttcg acggcaaccc ggagatgacc aagcagctcg tctcggcgat cctgctcggc
      601 gggcacatcg tggtcccgac ggggcggccg gacggcggag gcagcgaccc gaaggcgacg
      661 ttccagcacg tgccggcctg ccagcacgcc accgacacgg cctgcgtggt ggcctattcg
      721 agcttcaagc ccggcggcca gacgccgggc agcgacgcgg acatcgtgcg ggtggccggc
      781 gacccgagtc accaggtgct gtgcgtgaac ccggcggccc tgctgaccaa cgcccaggga
      841 ccggtgccac tggacaccgt cctgtacacg cgggaactgg tcggcggcaa cgatctcaac
      901 ccgtacggcc ggatcagtgg cctcgggcag tcgctgacgg tgccgaacta cccgaccggc
      961 ttcgcccgct actacggcac gctgaccggc cagtgcaagc aggcgaccga cagcaacggc
     1021 acggtgtcgt ggctggacat cgtcggcggc gacaacctgg tcggcccgcc gcaggccaac
     1081 ttctacggcc tgcacgcgct ggacttcaac ctcgccgcgg gcgacctcgg caccctgatc
     1141 gccaagcagg cggcggcctg gtcggcgggt acgtaacttc gctgcccggc cccgattacg
     1201 taccggatcg cattgtcgcg ttcacccgga cgccgccagc attgccggca tggggacgaa
     1261 gcaggacgtt gtccggcagg cggagagctg ctggggcatt tcgcacgccg agcgccaccg
     1321 ggcggacgcc gtggagatca cggaacgggc ggtggcggcg ctgcgggagc gggccgccgc
     1381 cgagccggac aacgccgacc acgagcagaa gctgtcctgg gcgctgatca acctggccca
     1441 gctgcactcg gctcgccgcg agcacgccaa ggccgacgcg gcggcgctgg agagcgtcgc
     1501 gatcctgcgc cggatgctgg cccgatgacc gccgcgctgt tgggcctgca ccgcgccgcg
     1561 gccactcttt cggcctattc ggcggcggtg cagctgggcc tgatcgacca catcgaccgc
     1621 accccggcca gcgtcgagga cctggcgatg acgtgcgggg caagcgttcg cggagtgcga
     1681 gtggtgctcg cgacgctggc cgacagcggc ctggtgcagc gccgcgccga cggccgctac
     1741 gagccggcga ccccggggct ggccggggtg cacccactgc tgccttggcg ggaccaggtg
     1801 accgactcgg tgcgcaccgg cctgccggcc cgcgaggccg agcgcgacgt gatcaccggg
     1861 ctgctgcacg ggatgcgcgc cgacgtggtc accgagctgc cggccgcccg gcgtgcgctg
     1921 gacgtcagcg tggacggggc accctgcggc gcggcgctgg ctgcccgcga cccgaagtgc
     1981 cgcgtcagcc tgctggacgt gccggccaat cgacggaccg actggtcgcc gcagttcgat
     2041 ttcctgcccg gcggcctgtt cgacgcccgc ccggtggccg gcgagtacga cctggtggtg
     2101 ctgcccggcg tctgccatct gctggacggc gccgccgccg ccgagctgat ccgacggctg
     2161 gccccggcgc tgcgctccgg cggcacgctg gcggtgatcg acacgctggc cggctcgcag
     2221 ggcgcggcca cccacgagct gtccctgctg ctgcgcaccc gcggcggcgc catccacgac
     2281 ccggccgact accgccgctg gctggcgggg gccggcccgc gtcgacgtgg gccggctgcc
     2341 ggcgctcacc gtgctgaccg ggctcaagcc ctagtccgat tggtgcatac tctgccgcgt
     2401 ggagtcgagg ctggttggcc ggcatcgtga gctggggatc gtcgaccggg cgatcgacgc
     2461 ggtccaaggc ggcgacggcc gcgtgctgct ggtgtccggc caggccggca tcggcaagtc
     2521 gcggctggcg atcagcgcgc tgcacaaggc caaggaccat ggcttcgcgg tgctgatggg
     2581 ctcgtccagc ccgttgcagg ccggcctggc ctacgccccg gtggtggagg cgctgcgccg
     2641 tcacctgggc tcgctccccg agccggagtc ggcacggctg ctggacggcc tgcacgacat
     2701 agccggcctg atcgccgatc ctcgcctgtc gccggccccg ctgtccggtg atccggagct
     2761 ggagcggacc cggatgttcg aggccgtgct gcgcctgacg gggcggatca ccgccaggcg
     2821 gccggcactg ttcgttgtgg acgatctgca ctgggccgac cgcggcacga tcgagctgct
     2881 gcaccacctg agccggggga gccgccgccg gctgctgctg tggttcggct accgcagcgc
     2941 cgagccgggc ggcccgctcg cggcgttggc gatgacagtg cgacgcgacg actcctcggt
     3001 cgagatggcg ttggaaccgt tgtcggacaa cgcggtcggc gaactggtcg gcgacctgct
     3061 cggcacgcgg ccgcaggacg acgtgctgcg caaggtgacc gcccgcgccc agggcgtgcc
     3121 gctgttcgtc acggcgctgg tcggcggcgg ggcgctccgg gacgccgccg ggctgccggt
     3181 gatcgtccgt gacgtggtgc tggatcgctt gcagcgtttg gacgatcggg cccggcggct
     3241 gctggagctg atcgccgtgg ccggggccgg cggctcgccg cggctgctgc gcgcggtgtg
     3301 ggccgaggac ggattcgacg ccgtgctgcg gaagttgttc gaccagggcc tgatcgagga
     3361 gctggtggac ggccctgccc aaagcttctc ggcactgacc taccgggtgg cgcatccgct
     3421 gtacgccgag gtcgcctacg cggaactcac ggtgggacaa cgacgttcgc tgcacgcggc
     3481 gatcgccgcc gagctgggcc cggccgacgt gctggaggcc gcgccgcact accgcgacgc
     3541 cggcgacatg gtcgacccgg tccgggcgag cgaagtcctt ggcgcggctg ggaaacgcgc
     3601 gctggacgtg cacgacgcgc acgaggccgt caactatctc agcgtggcgc tgaaccacgc
     3661 gaaggccgcc gggcgcgctg acctggtgcc ggacctgatg accgacctgg ccttcgcgtt
     3721 gcaggccagc ggccgcctcg acgacgccgc cttgatgtgg gacgacgcgg actccatcgc
     3781 gcagagcgcg ggcgacacgc tgcgccagcg gctgctcggc tactaccggt cgatgctgga
     3841 ggctgagcgc ggccatccgg cggagctgcc ccggccggtg ccgttcgacg acgacgaccc
     3901 gccgatcggg ctgatggtgc actggctggt ggcggtccgg gccggcgaca cggccacgat
     3961 gtggcaggcg ctggaccaga tgatcaacct ggcccggctc cgggacgacg acctggcccg
     4021 gtcggtcgcg cacttcggcg cgggcatggt ggcccgcctc ggcctcgact acgccggtgc
     4081 gcacacccag aacctgatcg ccctggagac ggccgagctc gccccgcccg atgtgcgcga
     4141 ccagtacacg tacacgccgc gactgctggc gccgagcctg gcgctgctcc gcggcgatcc
     4201 cgcgggcggg gtgcgggccg ccgacaatcc gggagccgtg tcggtgcagg tctttctgcc
     4261 gagcgcggtg cactatgtcg cctacgtgag gactttcgcg ctgttcgcgg ccggcgacct
     4321 gcccggggcg ctggccgtcg ccgacgagag cctggcgctg gccgagcggg tggtcgggca
     4381 ggaccggctg gtcggccggg cgctggccgc ccgcgcgttc ctgttggtgg agaacggcga
     4441 tctggcccgt gccgagcggg acctcaagga cgcgcgggcc gactacgaca ccacgtacga
     4501 cgacttcgtg ctggcggtcg acctcgccga gctcgcctac gcgctgcgca ccggcgcggc
     4561 cgtcgacgac tcggctttcc tcccgcaccg gccgctgcgc gacctcaccg tcgactggct
     4621 acgggtcgcc tactccggct acctggccgt cgcacgcaag aatcacgctg tggcgcaaca
     4681 ggttctggac gacctgcgca acggcgccaa gacctcgccg ttcctcgacg cgctggccgt
     4741 ccggcaggaa gggctgatgc tggacgaccc cgagcggctc gaccgggccg ccgcgcggct
     4801 ggcggcgatg ggcgccccgc tgcccgtgcc gaaaccgggc acaccgccgt tgagcaagcg
     4861 ggaacgggag atcgtgcggc tggtcggcgc ggggctgacc aacgggcaga tcgccgagcg
     4921 gctgttcctc agcgagcgca ccatcgagac gcacctgcac aacagctaca agaagctgcg
     4981 gctgaccacc cgcccggcgc tcacccggtg ggcgcttgag cacgccggcg actgacccgc
     5041 agccgccggc cgtcggtcga ccgctccagc cccggcacga acacgttcac cacggccagg
     5101 ttcctggtcc cgtactgctc ccgcgcgtac ggggtgaatc ccttgtcgcg caacatgttc
     5161 accagcaggt ccaggtgttc gcggggcgtg gtcggctgct tggtctcgtc gaagccgacc
     5221 tccgccgcgt ccggcagccg gtcggtgaag tcgggaatcg gccgggtgac gggggcgctc
     5281 tggccctgga tcagctcggt cagggcccgg gccgccgcga cccggctgcc cagcgccgcc
     5341 gcgctgccgg ccgctgccgg ccgttcgccc gaagccggcg cctggtacgc gtagaaggcc
     5401 ggcacgtcca ggtcggaggt catgtcgatc aggtgcacgg agcgtcccga ccggtcggcc
     5461 gcggtgacca gcagctcggc caggtccgcc ggcagcgccg ccgggtcgac cacccgtagc
     5521 ggtcgggagc ggtcggccag cagctgggcc agcgcgtccc gctcgatcgt ctcgttgacc
     5581 gcgtgcacca gcgcctcggc ggcgtacgcg ccggcggccc agccgaccgc ctcgatgcgg
     5641 gggtcggccg cggtgtagtc gtagaagtcc tcgacggtga agcgggccga ctccccggat
     5701 tccccgcggt agtccgggat ggtcaggaac accggcacgt gcgcgtcggc gctgccgtcc
     5761 agcgcctcgt agcggacgca gcccagttcg cgggccgggc cgtcggcgag ctgggcgatc
     5821 agcgggtccg cggccagcgg gccgtgcgcc acctcgtgcg cgtcgtgcag gcggacctgc
     5881 cggttgcgca ggccgtccag gccgcacagg tagtgctcca gggcctcgaa cagcgccccg
     5941 accttggccg tctccgggtc gccgtggccg aggccgtcgg ccgcctgcac cttcacgcag
     6001 ccgtaccgca gcgtgcactg ccagctgccc gggttcgcgt ccagcagggg agtgaagtcc
     6061 gggctcaggc ccagctcccc ggcggcggcc aggccacgcg cgagcgcggc gtcgaggggc
     6121 agaccccgtt cggccggtac cggcatcacg tgcaacgtca actccgtcct gctggatgcg
     6181 cccccctggg tacgcagtgc aggagcggcg ggggcggttc cagatacgcc cacgctgaca
     6241 tccgtgccga ctttttccaa ggtgtgtact aagccctgct cagcttttgg gtcgaacggg
     6301 cagtggttgg cctccctccc ggacagatcg ccacacttga ccaactggtt ggtcagacaa
     6361 cgagatccgg tctcagatca ccaggtcaga gccgttacaa ctggttgacc cgccccttgt
     6421 caactctggc cgagtccgtg tccgccacac ggagaagatt gtgtcgtcaa acagttgccg
     6481 tatgcacggc tgattcgcca ctgagcgcca catccagccg gccgaggcac catgggcttg
     6541 ggtggccggc gatcgacgga gaggtgtggt caacgtgcga gtgctcgtct cggtcgacat
     6601 ggagggcatc gccggggtgg tccacagcga tgacatccaa ccaggtcatc gcgagtacga
     6661 gcgcaaccgc gcgctgctga ccgcggaggc caatgccgcg gtgcggggcg tctacgccta
     6721 tgcggcggac gcgcaggtgc tggtcgctga cgcgcacgca caattccgga acctgttgcc
     6781 cgagctgctg aaccagcggg ccgaactgct gcgggggacg cccaggccca acgggatgct
     6841 ggccgggctc gacgccgaca tcgacgccgt gttgttcgtc ggctaccacg gcaaggccgg
     6901 cgcggcccgt tcggttctgg cgcacactgt ttccggccgg gtcattgccg atgtgcgttg
     6961 cgatggttcg tccctcggcg aactcggcct caacgccgcg ctggccgctc actacggggt
     7021 gccgtcggtt ctggtcagcg gcgacgacac cgttgccgcc gaggcggtcg aggtggcggc
     7081 gggtatgcgc tgtgtggtgg tcaagcgggc gctcggcgcc cgcgcggcga ggatgatcca
     7141 tcccgaggag gcgtgccggc gaatcgagct cgaggtgccg ctggcgctgg ccgaccgggc
     7201 cgccgtgcgc gcacggcggt tcgagggcct ggtgcggctg gaggtccagg tgcatcggcc
     7261 gtacatggcc gaacacgccc tgctggtgcc cggcatggaa cgcgtagacg gatgcacttt
     7321 gcgctacgaa gcgccggact tcccgaccgc gtacaacatc gtcgagctca tcgccgttct
     7381 cggctcactg tgatcgtgcc gaggacgacg gcgcggggcg tggcgggcca cgagtgagac
     7441 gcgctgggcc gtggtgggac tgccgacgcg ggcgttcccc ggatcaggtg cagaccgcac
     7501 acttgatcct gcgcgttggg aatcgcatca cgggcgtcct ggccaatggc tgaggaggcc
     7561 cacatcaggg ggtacatgac atgtcgtacg actccacgcc agacactctt tggcacagcc
     7621 tgcgagtcgc cgagctgatg ggcgagctga tcaaggagtt gctcgaccgg tcggtccgac
     7681 acgacctgag caagacccgc gagccggagc gggccgtcta cgacgaggtc gtgccgcaac
     7741 tgcgcgccac gacctacggc agcgtggagt accggaccct ggtcgacgcg atgggcgagg
     7801 ggctgcgaca ccattacgcg cacaaccgtc atcatccgga gcactttccc gacggaatca
     7861 acggcatgac cttggtcgac ctgcttgaga tgctggcgga ctggaaggcg gccaccgaac
     7921 gtgcctcgca cggtgacctc gccgacagcc tgacgatcaa ccgcgaacgg ttcggtatcg
     7981 caccacagct gatggacatc ctggcgaaca ccgctcgcca cttcgggtgg ctggccacgg
     8041 agccagaccg aaacgcagcg ccatgacaac ggcgtggccg ccgagatcgc cagtagccac
     8101 atcatcgacg aggcgtccga acactctctc acgccgccga gaggtggttg ctcgctcata
     8161 ccgatgaccg cccgtacgtc gaggtagggg ccacctacgc tgttggcatg accgagatcg
     8221 gttcgagggc acgcaggctg cccgcacctc cattcgtcgt ctgggggtcg ctcacggagc
     8281 cgcaccggcc aggaacacgg ccctggctca acttgctgcc cgacgaagtc gaacccagag
     8341 tcctggaggc agacaagccc aaccgcgtcg tgtggtcatc cctttggcca ggcagaccca
     8401 acgaccaggt gcacttggag ctgacggctg ctcggggcgg agagacctcg ctgacgttca
     8461 ccttggtcac ccctgacgaa gcccctgacc agagcaaaac cggccacctt cggcggaggc
     8521 tcaaccaact gctcttcgct gaccttcgtt actcctacgg ccagtgaccg ccgcaactcc
     8581 cggacatcgg cacatgccgc gttcagtgct gaccttggcc tcggaggggt cgccgatggt
     8641 ggtgcatttc aggtcaactc gccggcactg ttttgctcgc gcaggctgtc gcctgagtac
     8701 gaccggcgga gcccgtccaa cacgagggtg atgacaccgt cggcctcggc ccgccacccg
     8761 gggcggtcat gggccgcacc gatgccgtgc agcgccatca tcacggcacc ggcactcacg
     8821 tcgtcgcgga cggcgccgtc cttcacggcg gcggccacca ggtcggtgac agcctgctcc
     8881 agcgcccggc tgccctcggc gagggcactc gagcgatcgg ccatgagcgt ggccagcgtc
     8941 cgggccaggc cctggtgggc gtccatgtgg tcgaccatgc cgcgcagaaa ggccgccaac
     9001 gcctccgccg cgggcagcgt ggcctgtagc tggcgggcgc ggtcgcacag cgtggcgact
     9061 tccccgtggt agaccgcctc ggccagcgcc tcccgggtgg ggaagtggcg gtacagcgtg
     9121 ccggtgccca ccccggccag gcgggcgaag tcgtcgaagc gcaggtcgaa gaagtcgccg
     9181 gcgtcgaaga cctcccgggc tgtggcgagc agggcctcgc gcttgcgttg ggcgtcggcc
     9241 cgcagtggct tgtcggcggt catgcgctgc ctcctgttga caaatggaga tgacctccat
     9301 attttggaaa gtggagatga tctccagatc gatcgtaccc cacgaggtgc agcgtgaaga
     9361 tcctgatgtt cggccgaggc gtgatcgcca cggtctacgg ctgggccctg caacaggcgg
     9421 gacatgacgt cgagttctac gtccgcccgg gccgcgcggc ggcgtacggg gacacggtgg
     9481 acctcgacct gttcgatatg cggcgccggg tgtgggggca gcgcgtcgtg gagaagtggc
     9541 cggtgcgtta ccgcgaggcg ctggagccgg accacgattt cgacttgatc gtgctgagcg
     9601 tgccgcacca ccgcctcgcg gaggcggcgg ctttcctggc cccgcgcgtc agcaaagcga
     9661 cggtgctggt cttcggcaac gtctgggccg agccgctggc cgcgatcggc gcacttcccg
     9721 tcgaccggat cgcctggggc tttccccagg ctggtggcgg tttcggcgcg gacggcgtgc
     9781 tccgcggggc gctgctgccg tcggtcgtct tcggcaccct cggccagccc ccgaccgacc
     9841 gggagcgggc cgtgcgccag gcgtttcgcg aggccgggtt ccggcttaag gagcggcctg
     9901 acttccgcgg ctggctgtgg gtccatttcg tgtcggatgc gggcctgttc tcgcagggcc
     9961 tgcggctggg ttccctgtct aagctggccg ggtcgacgag cgacttccgt gaggcgctgc
    10021 tgaccggccg cgaactgctg ccgcttctcc aggcgcgcgg cattgacctg cgacgtcacc
    10081 ggggcggtgt gctgctgttc cgggtgccca cctggctgat ggcccccgcg ctcgcctggc
    10141 tggctgctca tgtcgcgccg gtgcgcgtga attttgcggc gcactccgcc cctgacgccg
    10201 aggagccgcg tgaggtctgc cgggacaccc tggccgaagc acgacggttg ggcgtcgcgg
    10261 tgccgcgact ggaggcggcg gaaccgagct tcgcccgcga ggggaagggc tgagccgaag
    10321 cgcttagccg gaaacgtcct cttctagccg ctgagcgggt gtttgtttca acggtcgccc
    10381 tcgttgtgtg tagtgctgtc tgccgtatcg gcggggcggg atttcattga gatgcggtga
    10441 tcgcggcaac gtgtaacgcg acggcacccc tcggttcatg agcgaagata cgtcctgaga
    10501 cttgacttcg accggtgatg tgcgggaccg ctgatcgggt atgtcgacgg aaccgacaac
    10561 ccgaatggtc tacgatggcc gaccagtgga atcacccgaa tggtccttgg gggtataggt
    10621 attttgggtc tgcactagct ccgctgaccg tggcaatcct cccttctgtt ggagtacccc
    10681 atgcgtagta gccgtgttct gcctattgcg atcttcgcaa tctgtggact cctgtcgtcg
    10741 gcggtcaccg cagtggcagg ggaagccagg acgaccggca gccgggccct gtcggcgtcc
    10801 ccagtgacgc agacgttcac cttcaccggt ggtgagcaga ccctggcgat accggtgggg
    10861 gtgacgcgtg tgacggtcac ggcggtaggc gccaagggag cgtctggcgc gagcgagtgt
    10921 ggcacgggcg gcgcgggagg cttcggcgaa acggccacgg cgacggtgtc ggtcaccgga
    10981 gggcaaacgc tgtatgtcga ggtcggcggc aacggtgcca ccaacgccag cgcgggcgcg
    11041 ggcggaggag gcggtttcaa cggcggcgga ggcggctcgg gcggcttcca gagcggcggg
    11101 acctcttcgg gggtctgcgc gggtagctcg gttggcggtg gcggtg
//
