LOCUS       NZ_CP060742            13060 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces alfalfae strain XN-04 chromosome, complete genome.
ACCESSION   NZ_CP060742
VERSION     NZ_CP060742.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     The Biocontrol and Plant Growth-promoting Properties of Streptomyces
            alfalfa XN-04 Revealed by Functional and Genomic Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2020) College of Plant Protection, Northwest A&F
            University, No. 3 Taicheng Road, Yangling 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141485.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,142
            Genes (coding)                    :: 6,967
            CDSs (with protein)               :: 6,967
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 75 of 175
            Pseudo Genes (incomplete)         :: 141 of 175
            Pseudo Genes (internal stop)      :: 22 of 175
            Pseudo Genes (multiple problems)  :: 59 of 175
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:18:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7125097
            Orig. end                         :: 7138157
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13060
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="28"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13060
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13060
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8060](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8060)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(838..852)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
     gene            855..1895
                     /locus_tag="H9W91_RS31590"
                     /old_locus_tag="H9W91_31580"
     CDS             855..1895
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378305.1"
                     /locus_tag="H9W91_RS31590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31580"
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="WP_076683343.1"
                     /transl_table=11
                     /translation="MNRKTTSADPELAHRLSRRGMLGVAAGATAAALVGGAAAPAAAGE
                     KGRGRPVLPPGRLGIQLYSLRDKISTLGFAPVFAELEKYGYDEIEFAGYTQGSAGAITL
                     AQLKALARDHGLHPIGSHVGYYDDNNPGAYTFAQNLDKVLDDAEALGLKHIGTASGPFR
                     YGSTVDGWKRAAEDFNTYGEAARKRGMKFYQHNHAEEFSFATDKPKVRLYDVLLAETDP
                     DFVYLEMDIYWAFCAQFRFSKRPDGRPARLDPLDYVLKQPHRYPLFHVKDGVRDETTRD
                     GYRMSDVGDGDIDYKRFLSKVTARTPQGRRYHHWQTEHDNPVDSLAFARKSSEHLHSLR
                     SGRCGD"
     tRNA            2049..2136
                     /anticodon=(pos:7127179..7127181,aa:Leu,seq:gag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="H9W91_RS31595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="H9W91_31585"
                     /product="tRNA-Leu"
     gene            2049..2136
                     /locus_tag="H9W91_RS31595"
                     /old_locus_tag="H9W91_31585"
     gene            complement(2201..2878)
                     /locus_tag="H9W91_RS31600"
                     /old_locus_tag="H9W91_31590"
     CDS             complement(2201..2878)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608085.1"
                     /locus_tag="H9W91_RS31600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31590"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_076688623.1"
                     /transl_table=11
                     /translation="MGHLKQKNLITARERLRDQVAHALRAALISGELRPGEIYSAPGLA
                     EDFGISATPVREAMLDLAREGLVEPVRNKGFRVTEVNERDLDQYTEIRLLIEVPMIGRI
                     TRTAAREDLEVLRPIADEIVRAAREHDLIGYLEADRRFHLSLLALSGNDRLVETVGDLR
                     KRSRLYGLTTLDARDQLTPSAEEHGELLDLMLTGDANAAEECMARHLGHVRSLWAQGAA
                     EGA"
     misc_feature    2950..2960
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(3030..4034)
                     /locus_tag="H9W91_RS31605"
                     /old_locus_tag="H9W91_31595"
     CDS             complement(3030..4034)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="H9W91_RS31605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31595"
                     /product="proline racemase family protein"
                     /protein_id="WP_076683341.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGVIPGATMADRRLHFIEHL
                     DHLRTLLMHEPRGHSAMSGALLQPPTRPDADYGVLYIEVSGVLPMCGHGTIGVATVLVE
                     TGMVPVTEPVTTVRLDTPAGLIRVDVSVTDGAATSATLTNVPAFCVALDRKIDVPGHGT
                     VTYDLAFGGNFYAFAHLDALGLPFTRDRKDDLLAAGLAVMAAINASPDRPVHPEQPDIA
                     GLKHVYLIAPGSDAHRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLERDFVNE
                     SFIGTEFTGRLIGRTEVAGLPAVVPRITGRAWLTGTAQYFLDPDDPFPAGFAL"
     gene            complement(4081..5004)
                     /locus_tag="H9W91_RS31610"
                     /old_locus_tag="H9W91_31600"
     CDS             complement(4081..5004)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237885.1"
                     /locus_tag="H9W91_RS31610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31600"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_076683339.1"
                     /transl_table=11
                     /translation="MTTTHPLTAAWSPSRPWRGIMVATALPLRDDLSVDYDAYAAHVAH
                     LLANGCDGVVPNGSLGEYQTLTDDERERVVRTAVEAAGDGARVMPGVSAYGSAESRRWA
                     EQAADAGCGSVLLLPPNAYRADERAVRAHYTEVAGAGVPVVAYNNPLDTRVDLTPELLA
                     RLHGDGAVVAVKDFSGDVRRAYETAELVPDLDLLVGADDVLLELALAGAVGWIAGYPNA
                     FPAACAELYRAACAHDLETALPLYRALHPLLRQDSGTEFVQSIKLSMDLAGRRGGPTRP
                     PRLPLGGPAEALVRAATEKALAAGHR"
     gene            complement(5001..6497)
                     /locus_tag="H9W91_RS36805"
                     /old_locus_tag="H9W91_31605"
     CDS             complement(5001..6497)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091457.1"
                     /locus_tag="H9W91_RS36805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31605"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_211827845.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.5e-21, bitscore:
                     75.6, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.7e-10, bitscore:
                     38.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPGDPYDVAVLGAGPAGLAAAVTAAEQGLTVALLDAAQQPGGQ
                     YYRHPAPAARATRPDALHHDWRVFAGLRERLDAAEGVTRLYGHHVWSMTREAPGSPWSL
                     HALTGEGDDDDASAPGTGTTVRARTMLLATGSHERHLPFPGWTLPGVVSAGGAQAMLKS
                     GLALPGTRVVVAGSGPLLLAAATSLAAAGADVPEVIEASGYLGYARHPAALAANPGKLT
                     EAARHGAALLRHRVRPRTRSAVTEAHGADRVEAVTVSRLGRDWRPVPGSERRVACDALA
                     VGHGLVPRTELATTLGCATRRAADGTYALELGPLQETSLRGLWAAGETSGVGGAQAALV
                     EGELAALAITARLTGRRPAPARRLRALRRRRDHLRAFADAMAAAHAPGPGWTRWLGDDT
                     EVCRCEEVTAARVREAVADYGARDPRSVKLLTRAGMGWCQGRVCGTAVACLAAAAPDAT
                     PAPPDRRLLATPVPLGTLASLTPADPPPAAPDDPSEGHPS"
     gene            complement(6481..6801)
                     /locus_tag="H9W91_RS31620"
                     /old_locus_tag="H9W91_31610"
     CDS             complement(6481..6801)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309276.1"
                     /locus_tag="H9W91_RS31620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31610"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_076683335.1"
                     /sec_met_domain="Fer2_4 (E-value: 7e-24, bitscore: 82.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQADRTTPADLVGATDATHFFTFDGRRVAARPGQSIAAALWTAG
                     ILAWRETRGAARPRGAFCGIGQCHDCLATVNGRPNRRACLVTARPGDEVTTQKGHGHAE
                     PR"
     gene            complement(6873..8060)
                     /locus_tag="H9W91_RS31625"
                     /old_locus_tag="H9W91_31615"
     CDS             complement(6873..8060)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891271.1"
                     /locus_tag="H9W91_RS31625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31615"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_076683333.1"
                     /sec_met_domain="DAO (E-value: 3.6e-70, bitscore: 236.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTRHPSGPPDFSDVIVVGAGMIGAACAHYASRAGLHVTVVDRGP
                     VAGGTTGAGEGNLLVSDKEPGPELRLALLSTRLWTELAAESPPDIEYERKGGLVVASTE
                     AELTALRALAAEQRRAGVTAADVPADRLADHEPHLAPDLTGGCHYPQDAQVQPALAAAH
                     LLRAAVGTGRLSLRLGEEVTAVLTEKGGAVRGVRTAKGEIRAPHVVNATGAWGGRLARM
                     AGVELPIRPRRGFVLVTEPLPRLVRHKVYSAEYVADVASGSAALQTSAVVEGTQAGPVL
                     IGASRERVGFDRALSVEALRRLASQATRLFPALARARALRAYAGFRPYLPDHLPAIGPD
                     PRVPGLLHACGHEGAGIGLAPATGLLITGFLTEAEPPLDPSPFAPERFAREPAPP"
     misc_feature    complement(8060..8076)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.54"
                     /tool="antismash"
     gene            complement(8136..8894)
                     /locus_tag="H9W91_RS31630"
                     /old_locus_tag="H9W91_31620"
     CDS             complement(8136..8894)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151713.1"
                     /locus_tag="H9W91_RS31630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31620"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_076683331.1"
                     /transl_table=11
                     /translation="MPENRRTIRHTPEAPTDTRSLAPGESIDAHRHDNHQIIYAGSGVV
                     AVTTDAGTWFAPGTRAIWVPAGCVHAHRAHGHLALHLLGLPADANPLGLDAPAVLTVSP
                     LLRELILACTRAPHEDTPERRRLRAVLLDQLRVSPQQPVQLPTPKDPRLAAVCGQLHRD
                     PADPRGLAELAATAGAGERTLSRLFRRELGMTFPQWRTQLRLYHALRMLADGVAVTTVA
                     HRCGWASASAFIDVFRRSLGYTPGAHQRGD"
     gene            9503..10642
                     /locus_tag="H9W91_RS31640"
                     /old_locus_tag="H9W91_31630"
     CDS             9503..10642
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151712.1"
                     /locus_tag="H9W91_RS31640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_31630"
                     /product="MFS transporter"
                     /protein_id="WP_076688621.1"
                     /transl_table=11
                     /translation="MVLLALAHACVDLYQGAVAALVPFFVAERAYTYAAASGVVLAASL
                     LSSVVQPVFGALTDRWAMPWLLPVSTLVGGIGVALAGLTGSYALTLAVVGLSGIGVAAY
                     HPEAARVARRASRGSHAAMSWFSLGGNLGFATAPLLVSAIAASGGLGASPLLVVPALAG
                     AVLCVAALRGVKAVEAATPKGVHREPGADDWPSFLRLSGAIVCRSIVFVGLGAFISLYA
                     RQRTGGGEAAGTAALFVLYIGGALGTVAGGRLAALFGRVPVVRWSYALTVPAVAGVVLV
                     PGPLFHVFVLLTSAGLYVPFSLHITLGQDFLPRRVGTAGGVTLGLTVSVGGLASPLIGA
                     LADATSLRTALAPLIALPALGWLLLRTLREPDGEAPRRA"
     misc_feature    10741..10757
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.29"
                     /tool="antismash"
     misc_feature    10745..10755
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(10749..10765)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.23"
                     /tool="antismash"
ORIGIN
        1 gcgggtgaac cgctccgtcg acatcaaccg cgtgctgcgc cgcgccgggc tgctcgacgt
       61 gcacacccag gacggcatgg aatacctcga cccgatggcc tcccgcgcct tcgccgtcgc
      121 cgaccaccag atcgcccacg tctacgtccg ccgccccgag gacctcgaag ccacccgcgc
      181 cgcactcgcc gacgtgcccg gcatcgagcg gctcctggac gacgagggca agaaggcgca
      241 tcagctcgac cacccccgcg ccggcgagct cgtcgccgtc gcggagcccg acgcctggtt
      301 cacgtactac tactggctcg acgacgaccg tgcgcccgac ttcgcgcagc tcgtcgagat
      361 ccaccgcaaa cccggctacg accccgtcga actcttcatg gaccccgagg acccctacgt
      421 ccgcgtgagg gcggcgagcg cgctggcccg caagaaactc ggcatgcgct accgcatggc
      481 ggtcgtgccc ctggacccct cacctattcg cggcagccac ggccgcctcc ccacgaacga
      541 ggacgaaagt ccgctcatcc tgtgctccac cccccgcgcc gtcgccggcc gcgtcgcggc
      601 caccgatgtg aaatcgctcc tgctccacct cgcgggcctg cagtgagagg ccgcgcgtcg
      661 ctgaagtcac ctggttccca ctgaaccaga gggtccgggc cctgccatca gggtccggcc
      721 ccgagtgatc cctgatgcgt tcttcgaacc catccccgac ccatccccga cacgaccccg
      781 accccgcccc gacccaccac cgacgtgacc tgacgcgccc ctgacaaacc cttgacctgg
      841 ccgaggagaa ccgcatgaac cgcaagacga cgtccgccga ccccgagctc gcacacagac
      901 tcagccggcg cggcatgctc ggtgtcgcgg cgggcgccac cgcagccgcc ctcgtcggcg
      961 gcgccgccgc cccggccgcc gcgggggaga agggccgggg ccgccccgtc ctgccgcccg
     1021 gacggctcgg catccagctc tacagcctgc gcgacaagat ctcgaccctc ggcttcgccc
     1081 ccgtcttcgc cgagctggag aagtacggct acgacgagat cgagttcgcc ggctacaccc
     1141 agggctccgc gggcgccatc accctcgccc agctcaaggc gctcgcccgc gaccacggcc
     1201 tgcaccccat cgggtcccac gtgggctact acgacgacaa caaccccggc gcgtacacct
     1261 tcgcgcaaaa cctggacaag gtcctcgacg acgccgaggc gctcggcctc aagcacatcg
     1321 ggaccgcgtc gggacccttc cgctacggct cgacggtgga cgggtggaag cgcgccgccg
     1381 aggacttcaa cacgtacggt gaggcggccc gcaagcgcgg catgaagttc taccagcaca
     1441 accacgccga agagttctcc ttcgccacgg acaagcccaa ggtccgcctc tacgacgtgc
     1501 tgctcgccga gaccgacccc gacttcgtgt acctggagat ggacatctac tgggccttct
     1561 gcgcccagtt ccgcttcagc aagcgccccg acggcaggcc ggcgcgtctc gaccccctgg
     1621 actacgtgct caagcagccg caccgctacc ccctcttcca cgtcaaggac ggcgtccgcg
     1681 acgagacgac ccgtgacggc taccgcatga gcgacgtcgg ggacggcgac atcgactaca
     1741 agaggttcct gtcgaaggtg acggcacgca ccccccaggg gcggcgctac caccactggc
     1801 agaccgagca cgacaacccg gtcgactccc tcgccttcgc ccggaagtcc agcgagcacc
     1861 tgcactcgct gcgctccgga cgctgcgggg actgaccccg cgcgcccggg cgcggagggg
     1921 cagggcctgg ggcccctccg tgcccgggcg ccgccggtgg acggatacgc aggtgaaagg
     1981 cactccgaaa ccttggtatt gttgtccatg tcgccgcggg gaacaccccg cgaggcggca
     2041 gacacctggt ccgggtggcg gaatggcaga cgcgctagct tgaggtgcta gtgcccttta
     2101 tcgggcgtgg gggttcaagt cccccctcgg acaccagcaa ccagcggcaa gaccgctgga
     2161 gcccagctga gacccctgtt caccaggggt cttctgtgtt ttacgctcct tctgccgcgc
     2221 cctgcgccca cagtgaccgt acgtgtccga ggtggcgcgc catgcactcc tccgccgcgt
     2281 tcgcgtcgcc cgtcagcatc aggtcgagga gttctccgtg ctcctcggcg gacggggtga
     2341 gctggtcgcg ggcgtcgagg gtcgtcaggc cgtagaggcg ggagcgcttg cggaggtcgc
     2401 cgaccgtctc gaccaggcgg tcgttgcccg agagggcgag cagcgagagg tggaagcggc
     2461 ggtcggcctc caggtagccg atgaggtcgt gctcgcgggc ggcccgtacg atctcgtcgg
     2521 cgatcgggcg cagcacctcc aggtcctcgc gggcggcggt gcgggtgatc ctgccgatca
     2581 tgggcacctc gatcaggagg cggatctccg tgtactggtc gaggtcgcgc tcgttcacct
     2641 cggtcacgcg gaaccccttg ttgcggaccg gctcgaccag gccttcccgc gccaggtcga
     2701 gcatcgcctc gcgcacgggc gtcgccgaga tgccgaagtc ctccgcgagg ccgggtgcgg
     2761 agtagatctc gccggggcgc agctcgccgg agatcagggc ggcgcgcaag gcgtgggcga
     2821 cctggtcgcg cagccgctcc ctggcggtga tcaggttctt ctgcttcaga tggcccatgg
     2881 tgtcccctcc ggtgatgccc gcgcccgtgg tgcctgcgcc tgtgcccgtg cccgcgcgga
     2941 gcaccaacgt acaatgtcac gttgtcagtt ggggcagggc ccgggacagg gcgcggggac
     3001 gggccgcggg agagggcccg gcgggcggct cagagggcga agccggccgg gaaggggtcg
     3061 tcggggtcga ggaagtactg ggccgtgccg gtgagccagg cccgtccggt gatccgtggc
     3121 acgaccgcgg gcaggcccgc cacctcggtc cggccgatga gccgccccgt gaactccgtg
     3181 ccgatgaagg actcgttgac gaagtcgcgc tccagcggga gttcgccgcg ggcgtgcagt
     3241 tgtgccatgc gggccgaggt gcccgtgccg cagggggagc ggtcgaacca gccggggtgg
     3301 atcgccatcg cgtgccggga gcggtgggcg tccgaaccgg gcgcgatcag gtagacgtgc
     3361 ttgagtccgg cgatgtcggg ctgctcgggg tggaccgggc ggtcgggtga ggcgttgatc
     3421 gccgccatga cggcgaggcc cgccgcgagc aggtcgtcct tgcggtcgcg ggtgaacggc
     3481 agccccaggg cgtcgaggtg cgcgaaggcg tagaagttgc cgccgaaggc caggtcgtag
     3541 gtgaccgtgc cgtgtcccgg cacgtcgatc ttgcggtcga gggcgacgca gaacgcgggg
     3601 acgttggtca gcgtggccga ggtcgccgcg ccgtcggtga cgctcacgtc gacccggatc
     3661 agtccggcgg gggtgtcgag gcggaccgtc gtgaccggct cggtgaccgg gaccatgccg
     3721 gtctccacca ggaccgtggc cacgccgatg gtgccgtgcc cgcacatggg cagcacgccg
     3781 gacacctcga tgtagaggac cccgtagtcg gcgtcggggc gggtcggcgg ctggagcagc
     3841 gcgccgctca tcgcggagtg gccgcgcggc tcgtgcatca gaagggtgcg caggtgatcc
     3901 aggtgctcga tgaagtgcag cctgcggtcg gccatggtgg cgccggggat caccccgacg
     3961 ccgccggtga tcacgcgtgt gggcatgccc tcggtgtgcg aatcgacggc gtggaagacg
     4021 tgacgggtgc gcatacggct ccttcgtcgg tcggtgggag cgccgggggt ctcggccggc
     4081 tcagcggtgg cctgccgcca gggccttctc ggtggccgcg cgcaccagtg cttccgcggg
     4141 tccgccgagc gggaggcgcg gcggtcgggt gggcccgccg cggcggcccg ccaggtccat
     4201 cgagagcttg atggactgca cgaactcggt cccggagtcc tggcgcagca gcgggtgcag
     4261 ggcccggtag agaggcagcg ccgtctccag gtcgtgggcg caggccgcgc ggtagagctc
     4321 cgcgcaggcg gcggggaagg cgttcgggta accggcgatc cagccgaccg cgcccgcgag
     4381 ggccagttcg aggagcacgt cgtcggcgcc gacgaggagg tcgagatccg ggacgagttc
     4441 ggccgtctcg tacgccctgc gcacgtcgcc gctgaagtcc ttcaccgcca cgacggctcc
     4501 gtcgccgtgg agccgggcca gcagctccgg cgtgaggtcg accctggtgt ccagggggtt
     4561 gttgtacgcg accacgggca cgccggcccc ggcgacctcg gtgtagtggg cgcgtacggc
     4621 ccgctcgtcg gcgcggtagg cgttcggcgg cagcagcagc acggaaccgc agcccgcgtc
     4681 ggccgcctgc tcggcccagc ggcgggactc ggcactgccg taggcggaga cgcccggcat
     4741 gacgcgggcc ccgtcgcccg ccgcctcgac ggcggtccgc acgacgcgct cgcgctcgtc
     4801 gtcggtgagg gtctggtact cgccgaggga gccgttgggg accacgccgt cgcagccgtt
     4861 ggcgaggagg tgggcgacat gtgcggcgta cgcgtcgtag tcgacggaga ggtcgtcgcg
     4921 cagggggagc gcggtggcga ccatgatgcc gcgccagggg cgggacgggg accacgcggc
     4981 ggtcaggggg tgcgtcgtcg tcatgaaggg tgcccttccg aggggtcgtc gggagctgcg
     5041 ggtggtgggt ccgctggggt gagtgaggcg agagtgccca gagggacggg cgtggcgagg
     5101 aggcggcggt ccggcggcgc gggggtggcg tcgggcgcgg cggcggccag gcaggccacg
     5161 gccgtgccgc acacgcggcc ctggcaccag cccatgccgg cgcgcgtgag gagtttgacg
     5221 ctacgggggt cgcgggcgcc gtagtccgcc accgcctcgc ggacccgggc cgccgtcacc
     5281 tcctcgcagc ggcacacctc cgtgtcgtcg ccgagccacc gtgtccagcc cgggcccggc
     5341 gcgtgcgcgg cggccatggc gtcggcgaag gcgcgcaggt ggtcgcggcg gcggcgcagg
     5401 gcgcgcaggc ggcgggccgg ggcaggccgc cgccccgtca gcctggcggt gatcgccagg
     5461 gccgccaact cgccctccac gagcgccgct tgcgccccgc cgacgccgga cgtctcgccc
     5521 gccgcccaca ggccgcgcag cgaggtctcc tggagcgggc ccagttccag ggcgtacgtg
     5581 ccgtcggcgg cgcggcgggt tgcgcagccg agggtggtcg ccagttcggt ccgcgggacc
     5641 aggccgtgcc cgacggccag ggcgtcgcag gcgacgcggc gttccgagcc ggggaccggg
     5701 cgccagtcgc ggccgaggcg ggagaccgtc accgcctcga cgcggtcggc gccgtgtgcc
     5761 tcggtgaccg cgctgcgggt gcgcgggcgc acacggtggc gcagcagggc cgcgccgtgg
     5821 cgggcggctt ccgtcagctt tccggggttg gcggcgaggg cggccgggtg gcgggcgtag
     5881 ccgaggtagc cggaggcctc gatcacctcg gggacgtcgg ctccggcggc ggcgagcgag
     5941 gtggccgcgg cgagcaggag cgggccgctg cccgccacga ccacacgtgt gccgggcagg
     6001 gccaggccgg acttcagcat ggcctgggcg ccgcccgcgc tgacgacgcc ggggagggtc
     6061 cagccgggga aggggaggtg tcgttcgtgg gagccggtgg ccaggagcat cgtgcgcgcc
     6121 cggaccgtcg tacccgtccc cggggcgctc gcgtcgtcat cgtccccctc gccggtcagg
     6181 gcgtggaggg accacgggga gccgggcgcc tcgcgcgtca tcgaccagac gtggtgaccg
     6241 tagagccggg tgacgccctc cgcggcgtcc aggcgttcgc ggaggccggc gaagacgcgc
     6301 cagtcgtggt gcagcgcgtc ggggcgggtg gcgcgcgcgg ccggggccgg atgccggtag
     6361 tactggccgc cgggctgctg ggcggcgtcc aacagggcca cggtcaggcc ctgttcggcg
     6421 gccgtgaccg ccgcggcgag gcctgccggc cccgcgccga ggaccgccac gtcgtacggg
     6481 tcaccggggc tcggcatggc cgtggccctt ctgcgtggtg acctcgtcgc cgggccgggc
     6541 ggtcaccagg caggcgcggc ggttggggcg gccgttgacg gtggccaggc agtcgtggca
     6601 ctggccgatc ccgcagaagg ctccgcgcgg ccgggcggcg ccccgggtct cccgccaggc
     6661 gaggatgccc gccgtccaga gagcggcggc gatggactgg cccggccggg ccgcgacgcg
     6721 gcggccgtcg aaggtgaaga agtgggtggc gtcggtcgcg cccacgaggt ccgcgggcgt
     6781 cgtcctgtcg gcttgcccca tgggggcggc tcctctcatg cggcggcttc cccgctgtgg
     6841 cggccatggc gtgctcgtct cccggagccg cctcacggcg gcgccggctc gcgggcgaag
     6901 cgttccgggg cgaagggcga cggatcgagc gggggctcgg cttccgtcag gaaaccggtg
     6961 atcagcaggc cggtcgccgg ggcgaggccg attcccgcgc cctcgtggcc gcacgcgtgc
     7021 agcagcccgg ggacccgggg gtcggggccg atggcgggga ggtggtcggg gaggtagggg
     7081 cggaagcccg cgtaggcgcg gagcgcgcgg gcccgggcga gggccgggaa cagccgggtc
     7141 gcctgggacg ccaggcgccg cagcgcctcc accgagagcg cgcggtcgaa gccgacccgt
     7201 tccctgctcg ccccgatcag caccgggccc gcctgggtgc cctccaccac ggcggacgtc
     7261 tgcagggctg ccgacccgct cgccacgtcg gcgacgtact ccgcggagta caccttgtgc
     7321 cgcaccaggc gcggcagggg ttcggtgacc aggacgaagc cgcggcgggg gcgtatcggc
     7381 agctccacgc ccgccatgcg ggccagtcga ccgccccagg cgccggtcgc gttgaccacg
     7441 tggggggctc ggatctcgcc cttcgcggtc cggacgccgc gcaccgcgcc gcccttctcc
     7501 gtcagcaccg ccgtgacctc ctcgccgagc cgcagcgaca ggcgtcccgt gccgaccgcg
     7561 gcgcgcagca ggtgggccgc ggccagggcc ggctgcacct gcgcgtcctg cgggtagtgg
     7621 cagccgccgg tgagatcggg cgcgaggtgg ggttcgtggt cggcgaggcg gtcggcgggg
     7681 acgtcggccg ccgtcacgcc cgcgcgccgc tgctccgccg cgagggcccg cagggcggtc
     7741 agttccgcct ccgtcgaggc cacgaccagg ccgcccttcc gctcgtactc gatgtccggc
     7801 ggggactcgg cggcgagttc ggtccacagc cgggtggaga gcagcgcgag tcgcagctcg
     7861 gggcccggtt ccttgtcgga cacgaggagg ttgccctcac ccgcgcccgt ggtgccgccc
     7921 gcgacggggc cgcggtcgac gacggtgacg tggaggccgg cgcgggaggc gtagtgggcg
     7981 caggcggcgc cgatcatccc tgctcccacg acgatgacgt ccgagaaatc cggtggtccg
     8041 gaggggtgtc tcgtgagcac agcagtaata tttcacatgg cactaaggct gccaagaggg
     8101 cgtgcgccac gaggagttgg ggtctggcgg tcgcttcagt cgccgcgctg gtgggcgccc
     8161 ggggtgtaac cgagcgagcg gcggaacacg tcgatgaacg cgctggccga cgcccagccg
     8221 cagcggtgcg cgaccgtggt gacggccacg ccgtcggcca gcatgcgcag ggcgtggtag
     8281 aggcggagct gggtgcgcca ctgggggaag gtcatgccga gctcgcggcg gaagaggcgg
     8341 ctgagggtgc gctcacccgc tccggcggtt gcggccagtt cggcgaggcc gcgcgggtcg
     8401 gcggggtcgc ggtgcagttg cccgcagacg gcggcgaggc gggggtcctt gggggtgggc
     8461 agctggacgg gctgctgcgg ggagacgcgg agctggtcga ggagcaccgc gcgcaggcgc
     8521 cggcgctccg gggtgtcctc gtgcggggca cgcgtgcagg cgaggatcag ttcgcgcagc
     8581 agcgggctga cggtgaggac ggccggggcg tcaaggccga gcgggttggc gtcggcgggc
     8641 aggccgagca ggtgcagggc gaggtggccg tgggcgcggt gcgcgtggac gcagcccgcg
     8701 gggacccaga tggcccgggt gcccggcgcg aaccaggttc cggcgtcggt ggtgacggcc
     8761 acgacgccgg aacccgcgta gatgatctgg tggttgtcgt ggcggtgcgc gtcgatggac
     8821 tcgccgggcg ccagtgaccg ggtgtccgtg ggggcctcgg gggtgtggcg gatcgtgcgg
     8881 cggttttccg gcatgggtcg gcactttatc ggaagcgcgc caggggtcgc gtggcgacga
     8941 tggagtgatg tcaacgatgg aacccacgag cgaactgccc tgcccggagg ccgacaaggc
     9001 ccgtcgcccg gagtccggca cgcgccctcg cccggagtcc gacaagggcc ctcgcccggc
     9061 ggccgacacg cgcactcacc cggagtccgg cacgcgccct cgcccggcgg ccggcacgcg
     9121 ccctcgcccg gcggccgaca cgcgccctcg cccggagtcc ggcacgcgcc ctcgcccggc
     9181 ggccgataag ggccctcgcc cggaggccga caaggccctt cgcccggagt ccggcacgcg
     9241 ccctcgcccg gcggccgaca cgcgcactca cccggagtcc ggcacgcgcc ctcgcccgga
     9301 gtccggcacg cgccctcgcc cggcggccga taagggccct cgcccggagg ccgacaaggc
     9361 ccttcgcccg gaggtcggca cgtgcactca cccggaggat acgaggccgg ccccggccgt
     9421 ggcggcaacc ggagcctcgg ccgctcccgg caccgaggcc cccacgcctc ccgagggcaa
     9481 ggccccgccg gcccgtcgcc ccatcgtcct cctcgccctg gcccacgcct gcgtggacct
     9541 ctaccaaggc gccgtcgccg ccctcgtccc cttcttcgtg gccgagcgcg cctacaccta
     9601 cgccgccgcc tccggcgtcg tcctcgccgc ctcgctgctc tcctccgtgg tgcagccggt
     9661 gttcggggcg ctcaccgacc ggtgggccat gccctggctg ctgcccgtga gcacgctggt
     9721 gggcggcata ggcgtcgccc tcgccgggct caccggcagc tacgccctga cgctcgcggt
     9781 cgtcggcctc tccggcatcg gcgtggccgc gtaccacccc gaggcggccc gcgtggcccg
     9841 gcgggcgagc cgcggcagcc acgcggccat gagctggttc tccctcggcg gcaacctcgg
     9901 cttcgcgacc gctccgctgc tggtctccgc catcgccgcc tccggcggcc tcggcgcctc
     9961 accgctcctc gtggtgcccg ccctcgccgg ggccgtgctc tgcgtcgccg cgctgcgggg
    10021 cgtgaaggcc gtcgaggcgg cgacgcccaa gggggtccac cgggagcccg gcgccgacga
    10081 ctggccctcc ttcctccggc tctccggggc catcgtctgc cgctcgatcg tcttcgtcgg
    10141 gctcggcgcc ttcatctcgc tgtacgcgcg ccagcgcacc gggggcggcg aagccgcggg
    10201 cacggccgcc ctgttcgtgc tctacatcgg cggcgccctc ggcaccgtcg cgggcggcag
    10261 gctcgccgcg ctgttcgggc gcgtcccggt ggtgcggtgg tcgtacgccc tgacggtccc
    10321 cgcggtggcc ggcgtcgtcc tcgtacccgg acccttgttc cacgtcttcg tcctgctcac
    10381 ctcggccggg ctctacgtgc cgttctccct gcacatcacc ctcggccagg acttcctgcc
    10441 gcgcagggtg ggcaccgcgg gtggcgtcac actcggcctc accgtgagcg tgggcggcct
    10501 cgcgagcccg ctgatcggcg ccctcgccga cgccacgtcc ctgcggaccg ccctggcgcc
    10561 gctcatcgcg ctgcccgcgc tcggttggct cctcctgcgc acactgcgcg aaccggacgg
    10621 cgaagccccg cgtcgcgcct gaagccccgc gtcgcgcctg aagcgcggac cctttcggtc
    10681 cccgcccccg aagcaccgca aggacctcgc gccccagcga attcggtgtc tgaggggttg
    10741 tcagtgcaat ttcacattac tacgttacgc atcacatggc ccggacgggt cctcgcgagg
    10801 ccgccgtccc ggagccgcca aacgtgctgc ccggcgcggc agttgtgcgc cctcgggccg
    10861 tcacacccca cacctcccac acaggagccc cgatgttccg gttacgccca agaaccctgc
    10921 gcagatccgt cctcgccggc gttctcgccg cgagcctctg cgtcacggcc gcgcccctct
    10981 ccgcccacgc ggccgacagc gccgcccccg cccgtgcgac ggccgccgag cccgactccc
    11041 gtgacgtcgt cgagcgcagg gcgtccgccc ccgatccggc cgcccgcgcg ctggccgcgc
    11101 ccggtcgcct caagaagggg caggtccccg ggccccgtac gccgaagccg aaggccgagc
    11161 agcgggccaa gggccaggac gggcccgcga ccgcgccctg cactctcgac ggcgtcacct
    11221 cgctcgggcc cgaggcgttc gccgacttcc tcgccgatcc cgccgtcacc gcggaaggct
    11281 gtctgcggac cctcatatgg acctgggacc ggcggctcgc ccccgtgatg tcggacgccc
    11341 acgtccaggc ggtgtccgga cgcgtcagcc ggctcgccgc cacgcacgac ggcaggaacg
    11401 gcaccaacct cctggaactg ctcacctacc tgcacgccgt cgcctaccag gacttctcgc
    11461 acgacgagat cgacgtcacc gacggcccca ccgtcgacgc catgcgccgc gccgtcgccg
    11521 ccttcggcgc ggcgccccgc acgttcgacg cgaccggcgc caacgccgac tccctgcgcg
    11581 aggcgctgta cgcggcgagc gctccggggc tgcggcagca ccaactgccg ctcgtcaaga
    11641 aggtcctggc gaccatggac gccgaccacc ccggcaccgc gaaggacgcc gcctggggcg
    11701 ggtccgcgct cgccgcgctc tccctgaact acctcggcgt ctaccccggc aaccgggaca
    11761 ccgccttcca cgcggcggcc aaggcggacc cggcttaccg cgcggccttc aaggcgttcg
    11821 ccgggcacac ccacctcaag ggcacgggca acgcctgggt ggtgcgggac gcgctcagtg
    11881 agtacggcag gttcgggcag atcgacgggg tcaggagcga gaccgtcagc ggtctcggcg
    11941 gcctcgtcgc cgtgaccgca cgcgacttcg gcgagggcag cgggccctgg gtgaaggtcg
    12001 cgacctggct gaacgagtac gacgcctgcg cgccgtacgg cgtctgccgg gcggacatcg
    12061 agcgccgcat cttcccgcac acgtacacct acgacaaggg cggcatgaag gtccgcacgg
    12121 cgctgggccg gggcgccgtc gaccagctct actacgcgag caagcaggtc aaggcgcagt
    12181 tccaccgcgt cctcggcgcc gatgagccac tggcgggcga ccccaacacc ggcctgacga
    12241 ccgtgctcta cgcctcccgc gccgactacg agaactacca ccccctgctc accggcatgg
    12301 gcaccgacaa cggaggcgtg tacatcgagc ggggcgcgac cttctacacc taccagcggc
    12361 gcgtgcccca ggactccacg ctcacgctgg aggaactctt ccgccacgag tacacccact
    12421 acctcaacgg ccgctgggcg gtccccggct tcttcggcga gggcccctgg tacgagaacg
    12481 accggaccac cgccatggac gaggggacgg ccgagttctt cgccggagcg acgcgcgacg
    12541 acggcgtgaa ggtccgcaag gcgctggtgc ggggcatcat cgacgacacg gcgaacggcg
    12601 gtccccggat gaccgtccgg cagatcctgc acgccacgta cgacggcgac ggcttccgct
    12661 tctacgacta cgcggggacg ttcttcgagt tcctgtgggc ggagcggccg agcctgctga
    12721 aggagatgta ccggcacctg cgcgccgacg acccggcggc cttcgacgcc tggcgcgacc
    12781 ggctcggcgg ggacggcgcg gtgcagcgcg cgtacgacgc cttcctcgac gagcagatcg
    12841 cgcacgtcga cgacctcttc gtgccggaca cgacgtacgt cccggtcggc gagctgcggg
    12901 acagcaccgc cgcccaggtc cgcacctcgt tcaggtcggc gacgggtgtc acgcccgagt
    12961 gccgctccaa cggggcgccg gaccggccgc gttacctctg cggcggccgc gtcaccgcga
    13021 acctcagcgc ctccgacgat ccggacgccg tcttcgagga
//
