LOCUS       NZ_CP060742            11284 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces alfalfae strain XN-04 chromosome, complete genome.
ACCESSION   NZ_CP060742
VERSION     NZ_CP060742.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     The Biocontrol and Plant Growth-promoting Properties of Streptomyces
            alfalfa XN-04 Revealed by Functional and Genomic Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2020) College of Plant Protection, Northwest A&F
            University, No. 3 Taicheng Road, Yangling 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141485.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,142
            Genes (coding)                    :: 6,967
            CDSs (with protein)               :: 6,967
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 75 of 175
            Pseudo Genes (incomplete)         :: 141 of 175
            Pseudo Genes (internal stop)      :: 22 of 175
            Pseudo Genes (multiple problems)  :: 59 of 175
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:18:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6546475
            Orig. end                         :: 6557759
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11284
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11284
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11284
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6284](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6284)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(378..1982)
                     /locus_tag="H9W91_RS29145"
                     /old_locus_tag="H9W91_29135"
     CDS             complement(378..1982)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (109-472). E-value:
                     1.3e-20. Score: 66.0. Matches aSDomain:
                     nrpspksdomains_H9W91_RS29145_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="H9W91_RS29145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29135"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_211827598.1"
                     /sec_met_domain="PF00561 (E-value: 2.1e-26, bitscore: 91.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAAARYGTAGTLALSALVVAPAQSASGATAPSAADRGVVVAAE
                     RAEAAGIRWGACPKAEDMPPSLKCGTVVVPLDYARPGGKQIRLTVSRARATGGDGRGKV
                     ARQGSLVYNPGGPGGSGMHFPVMGIMPEWKRVAAAYDLVGYAPRGVGRSAPLSCQNPER
                     FIKAPTSAPSHPGAAYKKARIAKAKAHARGCAARNPGLRHYTTLNNARDLDVLRAALGE
                     RRLTFVGASYGTYLGAVYATLFPSHVRRMVFDSAVNPDPAQIWYRNNLDQSAAFERRWA
                     DFRAWMAKHHAAYGLGRTPEAVMRSYDRANRKLAREPAGGKVGPGELQAAFLQAGYYDD
                     YWPMRAAALSQYLKGNPEPLVEQAAPEPRAAAEAENVNAVYTAVECNDAPWPTDFGTWD
                     RDNSLLARSAPFETWENVWMNLPCAYWPAPRQQPVDVRTAEGELPPTLILAGERDAATP
                     YEGALEMRRRMGGSALVTERGTGTHGIGGGGNACVNRHLEAYLLKGRVPARDASCAPRR
                     EPEPQTAERAARPAEAL"
     aSDomain        complement(567..1655)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS29145_Abhydrolase_1.1"
                     /evalue="1.30E-20"
                     /label="H9W91_RS29145_Abhydrolase_1.1"
                     /locus_tag="H9W91_RS29145"
                     /protein_end="472"
                     /protein_start="109"
                     /score="66.0"
                     /tool="antismash"
                     /translation="LVYNPGGPGGSGMHFPVMGIMPEWKRVAAAYDLVGYAPRGVGRSA
                     PLSCQNPERFIKAPTSAPSHPGAAYKKARIAKAKAHARGCAARNPGLRHYTTLNNARDL
                     DVLRAALGERRLTFVGASYGTYLGAVYATLFPSHVRRMVFDSAVNPDPAQIWYRNNLDQ
                     SAAFERRWADFRAWMAKHHAAYGLGRTPEAVMRSYDRANRKLAREPAGGKVGPGELQAA
                     FLQAGYYDDYWPMRAAALSQYLKGNPEPLVEQAAPEPRAAAEAENVNAVYTAVECNDAP
                     WPTDFGTWDRDNSLLARSAPFETWENVWMNLPCAYWPAPRQQPVDVRTAEGELPPTLIL
                     AGERDAATPYEGALEMRRRMGGS"
     gene            complement(2161..2943)
                     /locus_tag="H9W91_RS29150"
                     /old_locus_tag="H9W91_29140"
     CDS             complement(2161..2943)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125238.1"
                     /locus_tag="H9W91_RS29150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29140"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_119271221.1"
                     /transl_table=11
                     /translation="MFWVPLLLLAWAATGLTCGRLCVAAARAATPGKAAPTGRRLTLYE
                     AAFLSGGPARVADLALVAMAGARRLLIAHTGWATVVDPEGRDDIERSVLGAIGPAGQSR
                     IAPIRRGAAGADAVRALADRLVAAGLAVPEGAGEGVAAGVRQVRAAAVAVLALGAVAVL
                     MPGQEPAGAGETPVAAWFALPLLLCAGCLLIARVEVHPYTRWASPAGQALLRTLAAGDD
                     RLTAVAVRGVSAVDDPGLRAAFSHRAGRTGREAAQRGH"
     misc_feature    2978..2996
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    complement(3005..3020)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     misc_feature    complement(3005..3022)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.61"
                     /tool="antismash"
     gene            complement(3051..3794)
                     /locus_tag="H9W91_RS29155"
                     /old_locus_tag="H9W91_29145"
     CDS             complement(3051..3794)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385411.1"
                     /locus_tag="H9W91_RS29155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29145"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_076684018.1"
                     /transl_table=11
                     /translation="MRSRISNGTGLVVTGLVATLVALLFPLWSYEDRSGTGLDRLDAET
                     VATDFGPLTALDRDFVAKVRLAGLWELPAGRQAEERGTTKAVRTAGEHLVAGHTFLDAR
                     VREVAARLRLELPDQPGRQQRQWLAELSAARGGTYDEKFADILRRAHGAVFPVVAEVRA
                     GTRNSLVRTLADDANTTVLDHITVLEKTGLVDFDGLARDAAPDQAPATRSPAPPGPTAS
                     PAPASPATPSPMYPLPPAASRPKPE"
     gene            complement(3941..4996)
                     /locus_tag="H9W91_RS29160"
                     /old_locus_tag="H9W91_29150"
     CDS             complement(3941..4996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049031.1"
                     /locus_tag="H9W91_RS29160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29150"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_211827599.1"
                     /transl_table=11
                     /translation="MSVAAVVYATAVLLSCVPLVMGVVTVRRGGSRHGEGSRAGDGAFV
                     RGTPEAAFLSGGPARAADAVIAGLHEDGRLVVAGPGVVGITRPVARNAAEQAVIDAHTA
                     APSGALHWLRAAVMRSRPVQETGDALAERGLVVRPGPRRTWRRWAGAQVFLTFIGLPTA
                     GILTALQYADDDYTEPDRLLDSVLHVLLPAAALGIGVGVVSLTLTSGQLTPAGRRALHQ
                     YVDSSRHLTGAAHLVATRGLPAAHPDLRSQLIAAARARGLAAPPSSPAPLPFQDPGLWS
                     AGAAAWCGSTTGFGGAGGGSACSGGGGGAACSGSGSACSGGSSCSGGGGSSCGGGGGGS
                     SCGGGGGGGSC"
     misc_feature    4973..4984
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: weak, score: 17.94"
                     /tool="antismash"
     gene            complement(5001..6284)
                     /locus_tag="H9W91_RS29165"
                     /old_locus_tag="H9W91_29155"
     CDS             complement(5001..6284)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073955.1"
                     /locus_tag="H9W91_RS29165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29155"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_076684016.1"
                     /sec_met_domain="DUF692 (E-value: 2.4e-98, bitscore: 327.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIADAVEAMPGIDWVEAVAENICPGHLPDSLLR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLTALAERAEALGSPLVTEHIAFVRAGGALTAT
                     QPLEAGHLLPVPRTRDALDVLCENVRAAQDALPVPLAVENIAALIAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPVKALEELPVEAIAYVHVAGGFERDGVWHDSH
                     AHPVPEPVLAILADLASRTAPPGVLLERDENFPGPAELERELDAIRATVKTSPAEPGAA
                     PDTVPPASAAARQRTALAQAALLSALVAGTPAPEGFDHTRLKVQSRALGAKRADVVAKV
                     APELPEILGAAYRAEFLGYARVRPMTGGYRHDALGFAEHLLLAGRPASPDARQRLSDWW
                     LDRSGPAPRSRRPAARLLHATRRVLLRR"
     gene            complement(6389..7159)
                     /locus_tag="H9W91_RS29170"
                     /old_locus_tag="H9W91_29160"
     CDS             complement(6389..7159)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643870.1"
                     /locus_tag="H9W91_RS29170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29160"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_076684015.1"
                     /transl_table=11
                     /translation="MRHVNGTALIIAALVATLGAIAFPVWSYADRSGTGEADLAAGTVP
                     TRWGPLSAADRDLLVKVRLAGLWEIPAGQQAVERAPTEAVKEAGDHLIVGHTDLDKRVR
                     SAAAQLGVELPNQPTAQQQGWLDELTEASGETYQRTFANLLRASHGKVFSVIAQVRDTT
                     RNSLVRQLATDSNQTVLDHITMLEATGLVDFDDIANGAAADATESPTGPPPPPSGAAPP
                     EAPPAGPEGEVSTTSRPSPGPPGRIDTGRPEPQM"
     gene            complement(7225..7980)
                     /locus_tag="H9W91_RS29175"
                     /old_locus_tag="H9W91_29165"
     CDS             complement(7225..7980)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128420.1"
                     /locus_tag="H9W91_RS29175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29165"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_232626883.1"
                     /transl_table=11
                     /translation="MSSDETTSTGTRDGGPAADSPFRHESTPRDEAPQFVLPLVARIER
                     AAPPARTDALETAARAVLVMLSDERSLGDGEWARAMRDWQDARIRKVVRRARGGEWRKA
                     EALPGITVTGKSAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPGGADATHPVLWMS
                     PDVDMSAGKAMAQAGHGAQLAWWELSAEDRTAWRDAGFPLSVRTAAPAQWRALTTSGLP
                     VVRDAGFTEIAPGSCTVVADHPALRTPRH"
     gene            8048..8965
                     /locus_tag="H9W91_RS29180"
                     /old_locus_tag="H9W91_29170"
     CDS             8048..8965
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602442.1"
                     /locus_tag="H9W91_RS29180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29170"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_076688779.1"
                     /transl_table=11
                     /translation="MERDPVLRLSGVGRRYGLRGPWVLRGVDLGIRAGALIRVQGGNGT
                     GKSTLLRLLAGVDAPSEGRLTQRPRTAFVPERFPAALPFTAVGYLTHLARVHGLARGAA
                     GRSAERWLERFGAGGHARTPLSELSKGGSQKVAVAQALLADPELLVLDEAWTGLDVPAR
                     AELDRAVAERTAAGGSVVFVDHDPRRLADVADETYAVVGGGLERRTEPPGPEGAGPRGP
                     VVVIVAQGPPGAALPAPVAAVAVGVGAGGAAGATRLTVPESRSDLVLRELLTARVPWHV
                     VRVEEPGAVPSPTSAPAAEEGTSR"
     gene            8962..9630
                     /locus_tag="H9W91_RS29185"
                     /old_locus_tag="H9W91_29175"
     CDS             8962..9630
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="H9W91_RS29185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29175"
                     /product="ABC transporter"
                     /protein_id="WP_076684014.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPVILYAVFLGVGVQSGQPVLDSLAY
                     AAAVLLPVAAWLVRICAGSEPPAARACTAAAAGPARAHLAALLTAFLAAVALGVTAVLV
                     VTAVSDAASTNARLSVPRLPAGAAGLLAMLVSALLGTAVGALTSRPLLRSAGKAVPAML
                     LGALLLLVTTGSPAKSVLTDLTTGSRDGTVPVPWGAVLAALAVAVAAFWSACALSSRRG
                     "
     gene            9748..10992
                     /locus_tag="H9W91_RS29190"
                     /old_locus_tag="H9W91_29180"
     CDS             9748..10992
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H9W91_RS29190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H9W91_29180"
                     /product="hypothetical protein"
                     /protein_id="WP_123985590.1"
                     /transl_table=11
                     /translation="MRGADGTSGVGRAMGVRRPRAAACAVLAVLLTASGCGNGSEPDGS
                     RTGGSGASRPESASPRWEFEHIADFSGGFTDVAALGEDDIWAVGTENNGASNAHLLHYD
                     GERWKREPLPEALGDTDYPPVLEEIGDDTLWLRPQKYATGDGVTPWVRWDGVRWSAVPN
                     PPPGNVGDLEAAGPRDIWALTGERTAVHFDGSRWTTTRLPHRTVDLAVAGPDDVWAVGS
                     RSTGPGTEQVHGEPYDQPASSHWDGTSWQQVETPQSRFDEPLPPEPGAGLLKVFALADG
                     EVRAYGNNTFNHGEVEDEPQEEFIRLRRADDRWVEQEEAPGGCALRVPVAQDDEGLFLD
                     GNWFLHDDGRCLKIKRHRLPVSTGARKGSGQTLWLEEIHRVPGTDTWLGAGKVQVTQSG
                     DPFGAPVLVRLKRAA"
     gene            complement(11000..11215)
                     /locus_tag="H9W91_RS29195"
                     /old_locus_tag="H9W91_29185"
     CDS             complement(11000..11215)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983655.1"
                     /locus_tag="H9W91_RS29195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_29185"
                     /product="hypothetical protein"
                     /protein_id="WP_062775595.1"
                     /transl_table=11
                     /translation="MADDESYCAAPGTDTAAAVRALGLGPDAAAEGPFAACVLCQEPTE
                     YPESHKGATLCPVCAWQEAERQACSG"
ORIGIN
        1 gttcaacaag tcgcacatcc cggccttcct ggccgacgag acgccgcgga actacatcag
       61 cgtctacccc ttcgtgcgct cctacgactg gtatctgctg cccgacgagg accgccgccg
      121 catgctcgcc gaccacggca agatggcccg cggctacccc gacgtgcgcg ccaacacggt
      181 cgcctcgttc tcgctcggcg actacgagtg gatgctggcc ttcgaggccg acgagctgta
      241 ccgcatcgtc gacctcatgc gccacctgcg tgcctccgag gcccggatgc acgtccgtga
      301 agaggtgccg ttctacaccg gccgccgcaa gtccgtcgcc gacctcgtgg cgggcctggc
      361 ctagatccga gggtccctca gagcgcctcg gcgggccgcg cggcccgctc ggcggtctgc
      421 ggctccggct cccggcgcgg cgcgcacgac gcgtcccgcg ccgggacgcg gcccttcagg
      481 aggtaggcct ccaggtggcg gttgacgcag gcgttgccgc cgccgccgat gccgtgcgtg
      541 cccgtgccgc gctcggtcac cagggccgag ccgcccatcc tgcggcgcat ctccagggcg
      601 ccctcgtacg gggtggccgc gtcgcgctcg ccggccagga tcagggtcgg gggcagctcg
      661 ccctcggccg tacggacgtc gaccggctgc tgccggggcg ccggccagta ggcgcacggc
      721 aggttcatcc agacgttctc ccacgtctcg aacggcgccg agcgggcgag gagcgagttg
      781 tcgcggtccc aggtcccgaa gtccgtcggc cagggcgcgt cgttgcactc cacggccgtg
      841 tagacggcat tgacgttctc cgcctccgcc gccgcccgcg gttcgggcgc cgcctgttcg
      901 accagcggct ccgggttgcc cttgaggtac tgcgagagcg ccgccgcgcg catcggccag
      961 tagtcgtcgt agtacccggc ctggaggaag gccgcctgga gttcacccgg gccgaccttg
     1021 ccgccggccg gctcgcgggc cagcttccgg ttcgccctgt cgtagctgcg catgaccgct
     1081 tccggcgtcc tgcccaggcc gtacgccgcg tggtgcttcg ccatccacgc gcggaagtcc
     1141 gcccagcgcc gctcgaacgc cgccgactgg tcgaggttgt tgcggtacca gatctgcgcg
     1201 ggatcggggt tcaccgccga gtcgaagacc atgcgccgca cgtgcgaggg gaagagcgtg
     1261 gcgtagacgg ccccgaggta ggtgccgtac gaggcgccca cgaacgtcag cctccgctcg
     1321 ccgagcgcgg cccgcagcac gtcgaggtcg cgtgcgttgt tcagtgtcgt gtagtgccgc
     1381 agccccgggt tgcgggcggc acacccgcgc gcgtgcgcct tcgccttggc gatgcgcgcc
     1441 ttcttgtacg cggcgccggg gtggctcggc gcgctcgtgg gcgccttgat gaaccgctcg
     1501 gggttctggc aggagagcgg ggcggagcgg ccgacaccgc gcggggcgta ccccaccagg
     1561 tcgtaggcgg ccgcgacgcg cttccactcc ggcatgatgc ccatgaccgg gaagtgcatg
     1621 cccgacccgc cgggcccgcc cgggttgtag accagcgagc cctgccgcgc gaccttcccg
     1681 cgcccgtccc cgccggtcgc ccgcgccctg ctgacggtca gtcggatctg cttgccgccg
     1741 ggccgcgcgt agtccagcgg cacgacgacc gtcccgcact tcagggacgg gggcatgtcc
     1801 tcggccttgg ggcaggcgcc ccagcggatt ccggcggcct ccgcgcgctc ggcggcgacc
     1861 acgacgcccc ggtccgcggc ggacggcgcg gtggctccgc tcgcgctttg cgcgggggcg
     1921 acgacgaggg cgctcagggc cagggttccg gcggttccat aacgggcggc tgctgctctc
     1981 atcgcgctga tgaccccttc ggttcgggcg tgcgttcccg caggcggtga cgtccgcgcg
     2041 cgcccgcgag ggctcgccgt cgggcgtcgg catcggcagc ggcgtcggcg tgggtgtcaa
     2101 acacagggga tgattcgtcc gatagtcggt gaagtaaagc accggcccgt cggtgtcggc
     2161 tcaatgaccc cgttgagccg cttcccggcc cgtccggccc gcccggtgcg agaacgccgc
     2221 gcgcagcccg gggtcgtcga cggcgctcac cccccgtacc gccacggccg tgaggcggtc
     2281 gtcgcccgcc gcgagcgtcc gcaggagcgc ctgccccgcg ggcgaggccc agcgcgtgta
     2341 cgggtggacc tcgacgcggg cgatcagcag gcagccggcg cacagcagca gcggcagcgc
     2401 gaaccacgcg gcgaccggcg tctccccggc gcccgcgggt tcctgacccg gcatgagcac
     2461 cgccacggcg ccgagcgcga gcaccgccac cgccgcggcc cgcacctggc gcaccccggc
     2521 cgccacgccc tcgcccgcgc cctcgggaac ggcgaggccc gcggcgacca gccggtccgc
     2581 cagcgcccgc acggcgtcgg cgccggccgc cccgcggcgt atcggcgcga tgcgggactg
     2641 ccccgccggg ccgatcgcgc cgagcaccga acgctcgatg tcgtcgcgcc cctccgggtc
     2701 gaccacggtc gcccacccgg tgtgcgcgat cagcagccgc cgcgccccgg ccatcgcgac
     2761 gagcgccaga tcggcgacgc gcgcgggccc gccggacaag aacgccgcct cgtagagcgt
     2821 gagacggcgg ccggtcggcg cggccttccc cggcgtcgcg gcacgggcgg cggccacgca
     2881 gagcctgccg caggtcaggc cggtcgcggc ccaggcgagg aggagcaggg ggacccagaa
     2941 catggcggtg ttctatgcga aggcgtcgct tcgcgccatg gcatattcag catgcgggac
     3001 gtcggtgacg ggtaggtaac gttccgtttc ccgccgggtg atggcggggg ttactccggc
     3061 ttcgggcggg aggcggccgg cggcagggga tacatggggg acggggtggc cggcgaggcc
     3121 ggggccggtg acgcggtggg gcccggcggc gcgggggagc gggtcgcggg ggcctggtcg
     3181 ggggccgcgt cccgcgcgag gccgtcgaag tccaccaggc cggtcttctc caggacggtg
     3241 atgtggtcga ggacggtcgt gttcgcgtcg tcggcgagcg tacgcaccag tgagttgcgg
     3301 gtgccggcgc gcacctcggc caccaccggg aacaccgcgc cgtgcgcccg gcgcaggatg
     3361 tcggcgaact tctcgtcgta cgtcccgccg cgcgccgcgc tcagctcggc gagccactgc
     3421 cgctgctgcc ggccgggctg gtccggcagt tccaggcgca gccgggcggc gacctcgcgt
     3481 acgcgcgcgt ccaggaacgt gtggcccgcc acgaggtgct cgcccgcggt ccgcaccgcc
     3541 ttggtggtgc cgcgctcctc ggcctgccgg cccgcgggca gctcccacag gcccgcgagc
     3601 cgcaccttgg cgacgaagtc ccggtccagg gcggtaagcg gcccgaagtc ggttgccacc
     3661 gtctccgcgt ccagtctgtc caggccggtg cccgagcggt cctcgtagga ccagaggggg
     3721 aagagcagcg cgacgagcgt cgcgacgagc cccgtgacga cgagaccggt gccgttgctg
     3781 atgcgggatc gcatgggcgc ctcctggtgc ggcaccgcac gctagtgccc cccgcgcggc
     3841 ccggggcggc gttacggcgg ggcgtacgcc aacctccgta cggcgccacc ggcccgggcg
     3901 gcgcggcgtg cgccgcccgt cgggaggagg gcggactgct tcaacaggac ccgccgcccc
     3961 cgccgccccc gcaggaggag ccgccaccgc caccgccaca ggacgagccg ccgccgcccg
     4021 agcaggacga gcctcccgag cacgcgctcc cgctgcccga acaggccgcg ccgccaccgc
     4081 cgccggagca cgcggagccg ccgcccgcgc cgccgaagcc ggtggtggac ccgcaccagg
     4141 ccgccgcccc ggcgctccag aggcccgggt cctggaacgg cagcggcgcc ggggacgagg
     4201 ggggcgccgc gaggccccgg gccctggccg cggcgatgag ctgggaccgc aggtcggggt
     4261 gggccgcggg gagcccgcgg gtggccacga ggtgggcggc gcccgtcagg tgccgggacg
     4321 agtccacgta ctggtggagg gcgcggcggc cggcgggcgt gagctgaccg gacgtcaggg
     4381 tgagggagac gacgccgacg ccgatgccga gggcggcggc ggggagcagg acgtgcagga
     4441 cgctgtcgag cagccggtcc ggctccgtgt agtcgtcatc ggcgtactgc agcgccgtca
     4501 ggatgcccgc ggtcgggagc ccgatgaagg tcaggaagac ctgagccccc gcccagcgcc
     4561 gccaggtgcg ccgcggcccg gggcgcacga cgaggccccg ctccgccagc gcgtcgcccg
     4621 tctcctggac cgggcggctg cgcatcacgg cggcccgcag ccagtgcagc gcaccgctcg
     4681 gcgccgccgt gtgggcgtcg atgaccgcct gctcggcggc gttgcgcgcg accggccgcg
     4741 tgatgccgac gacgccgggg cccgcgacga ccaggcggcc gtcctcgtgc agccccgcga
     4801 tgaccgcgtc ggcggcgcgg gcggggccgc cggagaggaa cgcggcctcc ggggtgcccc
     4861 gcacgaaggc cccgtcaccg gcgcgggagc cctcgccgtg ccgggacccg ccgcggcgga
     4921 ccgtcaccac gcccatgacc agcggcacgc aggagagcag gacggcggtc gcgtacacga
     4981 ccgcggcgac gctcatggcg tcaccgccgc agcaggacgc ggcgggtggc gtgcagcagc
     5041 cgggcggcgg gccgccggga gcgcggcgcc gggcccgagc ggtccagcca ccagtcgctc
     5101 agccgctgcc gggcgtccgg tgacgcgggc cgccccgcga gcagcaggtg ctcggcgaag
     5161 ccgagggcgt cgtggcggta gccgccggtc atcggcctga cccgggcgta gccgaggaac
     5221 tcggcgcggt aggcggcgcc gaggatctcc ggcagctccg gcgcgacctt cgcgacgacg
     5281 tcggcgcgct tggcgcccag cgcgcggctc tgcaccttca ggcgcgtgtg gtcgaagccc
     5341 tcgggcgccg gggtgcccgc cacgagtgcg gagagcaggg cggcctgggc gagtgcggtg
     5401 cgctgccggg cggcggcgga ggcgggcggc acggtgtccg gcgcggcgcc cggctcggcg
     5461 ggggaggtct tcaccgtggc ccggatggcg tcgagttcgc gctccagctc ggccggtccg
     5521 gggaagttct cgtcccgttc gagcaggacg ccgggcggcg cggtgcgcga cgcgaggtcg
     5581 gccaggatcg cgaggaccgg ctcggggacg ggatgggcgt ggctgtcgtg ccagaccccg
     5641 tcccgctcga agccgcccgc gacatggacg tacgcgatgg cctcgaccgg cagttcttcc
     5701 agggccttga ccgggtcctc gccgcggttg acgtggttgg tgtggaggtt ggcgacgtcg
     5761 atgagcagcc gcacaccggt gcggtcggcc agctcgtaca ggaactgccc ctccgtcatc
     5821 tcctcgccgg gccaggcgat gagcgccgcg atgttctcca cggcgagcgg cacgggcagc
     5881 gcgtcctggg cggcgcggac gttctcgcag agcacgtcga gcgcgtcgcg ggtgcggggc
     5941 accggcagca ggtgcccggc ttcgaggggc tgggtggcgg tgagggcgcc accggcccgt
     6001 acgaacgcga tgtgctcggt gaccagcggc gagcccaggg cctcggcccg ctcggcgagc
     6061 gccgtcagac gcccctcgtc gggacggtcc gcgccgccga ggcccagtga gacaccgtgc
     6121 ggcacgacgg tcacgccacg ctcgcggagc cgcagcagcg agtcgggcag atgccccgga
     6181 cagatgttct ccgcgacggc ctcgacccag tcgatgccgg gcatcgcctc cacggcgtcc
     6241 gcgatctcag gacgccagcc gataccggtc cccagacgct ccatttctcc cccttcgtcc
     6301 ccccgagttc cccggtcgcc cggtcactcg tccccgggac catcaccggg acgggaccgg
     6361 ccgaaccccg caggggagac gttcagagct acatttgagg ttccggccgg ccggtgtcga
     6421 tgcgtcccgg cggccccggc gagggccgcg acgtggtcga cacctcgccc tcgggcccgg
     6481 cgggcggagc ctcgggcggg gcggccccgg acggcggggg aggaggcccg gtggggctct
     6541 cggtggcgtc ggccgcggcg ccgttggcga tgtcgtcgaa gtcgacgagc ccggtggcct
     6601 ccagcatcgt gatgtggtcg aggacggtct ggttggagtc cgtggcgagc tgcctgacca
     6661 gcgagttgcg ggtggtgtcg cggacctggg cgatcacgct gaagaccttg ccgtgcgagg
     6721 cgcgcagcag attggcgaac gtgcgctggt acgtctcccc gctcgcctcc gtcaactcgt
     6781 ccagccagcc ctgctgctgg gcggtcggct ggttgggcag ctccacgccg agctgcgcgg
     6841 cggcggagcg cacccgcttg tcgaggtcgg tgtggcccac gatgaggtgg tcaccggcct
     6901 ccttgacggc ctcggtcggc gcgcgctcca cggcctgctg gcccgcgggg atctcccaga
     6961 gcccggcgag ccgcaccttc accagcagat cccggtcggc cgccgagagc ggcccccacc
     7021 gggtgggcac cgtcccggcg gcgaggtcgg cctcgcccgt gccggagcgg tcggcgtagg
     7081 accacacggg gaacgcgatc gcgcccagcg tggcgacgag tgccgcgatg atcagagccg
     7141 tgccgttgac gtgccgcaag gtgcctcccg tgctcccgtg ctcaccggtg ataccgagga
     7201 gtacggaaga cacgggcggg gcgttcagtg gcggggcgtg cggagcgccg ggtggtcggc
     7261 gaccaccgtg caggagcccg gggcgatctc cgtgaagccc gcgtcgcgga ccaccggcag
     7321 gccgctcgtg gtcagcgccc gccactgggc gggggcggcc gtgcggaccg agagggggaa
     7381 gccggcgtcg cgccaggccg tgcggtcctc ggcggacagc tcccaccagg cgagttgggc
     7441 gccgtggccc gcctgcgcca tcgccttgcc cgccgacatg tcgacatcgg ggctcatcca
     7501 caggacgggg tgcgtcgcgt cggccccgcc cggcggctcc gggtcgtcca ggtcggtgcc
     7561 cgacacctgg agtttcgcca gctccttggg ccagccgtcc agcgggaccg gcgggaagac
     7621 gcgcacctcc gcggacttgc ccgtgaccgt gatgccgggc agcgcctcgg ccttgcgcca
     7681 ctcgcccccg cgcgccctgc gcaccacctt gcggatgcgg gcgtcctgcc agtcgcgcat
     7741 cgcccgcgcc cactcgccgt cgccgagcga acgctcgtcg ctcagcatca ccaggacggc
     7801 acgggccgcg gtctccaacg cgtccgtgcg ggccggggga gcggcacgct cgatgcgggc
     7861 gacgaggggc aggacgaact gcggggcctc gtcgcgcggt gtgctctcgt ggcggaaggg
     7921 gctgtccgcg gcgggcccgc cgtcccgcgt gccggtggaa gtcgtctcgt cgctgctcac
     7981 gggcccagtc tgccaggcgg ggaatccgcc ccgcggggcg tcggccgcgt acgcgaggat
     8041 ggcccgcatg gaacgtgacc ccgtgctccg gctcagcggc gtcggccgcc gttacgggct
     8101 gcgcggcccc tgggtgctgc ggggggtgga cctcggcata cgggcgggcg ccctgatacg
     8161 cgtccagggc ggcaacggga ccgggaagtc gaccctgctg cggctgctcg ccggtgtcga
     8221 cgcgccctcc gagggccggc tcacccaacg cccgcgcacg gcgttcgtgc ccgaacgctt
     8281 ccccgcggcg ctgccgttca ccgccgtcgg ctacctcacg cacctggccc gcgtgcacgg
     8341 cctcgcgcgc ggcgcggccg ggcggagcgc cgagcggtgg ctggagcgct tcggcgccgg
     8401 cggccacgcg cgcaccccgc tgtccgaact gtccaagggc ggcagccaga aggtggccgt
     8461 ggcgcaggcc ctcctcgccg atcccgagct gctggtcctc gacgaggcct ggacgggcct
     8521 ggacgtcccc gcccgcgccg aactcgaccg cgcggtcgcc gaacgcaccg ccgccggcgg
     8581 ctccgtcgtc ttcgtcgacc acgacccgcg ccgcctcgcg gacgtggcgg acgagacgta
     8641 cgcggtggtg ggcggcggac tcgaacggcg cacggagccg ccgggcccgg agggcgccgg
     8701 gccgcgcggt cccgtcgtcg tcatcgtcgc gcaggggccg ccgggcgcgg cgctccccgc
     8761 gccggtcgcg gccgtcgcgg tcggcgtcgg cgccgggggc gcggcggggg ccacgcggct
     8821 cacggtcccg gagagccgct ccgacctggt cctgcgtgaa ctcctgacgg cacgggtgcc
     8881 ctggcacgtg gtgcgggtgg aggagccggg cgccgtcccg tcccccacct cggcgcccgc
     8941 cgccgaagag gggacctccc ggtgaccgcg ctcctgcggt accaggccgc cctgctcgca
     9001 cggtcccagc ggtggctcgc gcccgtcatc ctgtacgccg tcttcctcgg cgtcggcgtg
     9061 cagagcgggc agcccgtcct ggactcgctc gcctacgccg ccgccgtgct gctgcccgtc
     9121 gccgcctggc tggtgcggat ctgcgccggg agcgaaccgc ccgccgcccg cgcctgcacc
     9181 gccgcggcgg ccggcccggc gcgcgcccac ctcgccgcgc tgctgaccgc gttcctcgcg
     9241 gcggtggcgc tcggcgtgac ggcggtgctc gtcgtgaccg ccgtcagcga cgcggcgagc
     9301 accaacgcca ggctgtccgt gccccggctg cccgcgggcg ccgcaggact gctcgcgatg
     9361 ctggtcagcg ccctgctcgg cacggcagtc ggggcgctca ccagccggcc gctgctccgc
     9421 tcggcgggga aggccgtgcc cgccatgctg ctcggcgcgc tgctgctgct cgtcaccacg
     9481 gggtccccgg cgaagtcggt cctcaccgac ctgaccacgg ggtcacgcga cgggaccgtg
     9541 ccggtgccgt ggggggcggt gctcgcggcc ctcgccgtgg ccgtggcggc gttctggtcc
     9601 gcctgcgccc tcagctcccg gcgcggctga cccccggggc gcggggcacg cccgggggcg
     9661 aggggggata ggctgcccgc cttctgcacg gcaacaacac cagcaacatc agcaacaccc
     9721 aacgagcgac aacggggagg gcgccggatg cgtggagccg acggaaccag cggagtcggc
     9781 cgggccatgg gcgtcaggcg cccccgcgcg gcggcctgcg cggtgctggc cgtcctgctg
     9841 acggcctcgg ggtgcggcaa cggctccgag ccggacggct cccggaccgg cggctccggc
     9901 gcctcacgcc ccgaaagcgc ctcgccccgc tgggagttcg agcacatcgc ggacttcagc
     9961 ggcggcttca ccgacgtcgc cgccctcggc gaggacgaca tctgggcggt cggcaccgag
    10021 aacaacggcg cctccaacgc ccatctgctg cactacgacg gcgaacggtg gaagcgtgag
    10081 cccctgcccg aggccctcgg cgacaccgac tacccgcccg tgctcgaaga gatcggcgac
    10141 gacaccctgt ggctgcggcc gcagaagtac gccacgggcg acggcgtcac cccctgggtg
    10201 cgctgggacg gcgtccgctg gtccgcggtg ccgaacccgc cgccgggcaa cgtcggggac
    10261 ctggaggccg cggggccgcg ggacatctgg gccctcacgg gcgagcggac cgccgtgcac
    10321 ttcgacggca gccgctggac cacgacgcga ctgccccaca ggaccgtcga cctcgcggtg
    10381 gcgggaccgg acgacgtgtg ggcggtcggc agccgttcga cgggccccgg caccgaacag
    10441 gtccacggcg agccgtacga ccagcccgcg tcgtcgcact gggacggcac gtcgtggcag
    10501 caggtggaga ccccgcagtc ccgcttcgac gagcccctcc cgccggagcc gggcgcgggt
    10561 ctcctcaagg tcttcgcgct cgcggacggc gaggtgcgcg cgtacggcaa caacaccttc
    10621 aaccacggcg aggtcgagga cgaaccccag gaggagttca tccggctccg ccgtgcggac
    10681 gaccggtggg tcgagcagga ggaggcgccc ggcgggtgcg cgctgcgggt cccggtggcc
    10741 caggacgacg agggcctgtt cctcgacggc aactggttcc tgcacgacga cggccggtgc
    10801 ctgaagatca aacgccaccg cctgcccgtg tcgacggggg cccgcaaggg ctccggtcag
    10861 acgctgtggc tggaggagat ccaccgggtg cccggcacgg acacatggct gggcgcgggc
    10921 aaggtccagg tgacccagtc cggcgacccc ttcggcgccc ccgtgctcgt acgcctgaag
    10981 cgcgccgcgt aacccggcgt cagcccgagc acgcctgccg ctcggcctcc tgccaggcgc
    11041 agaccgggca cagggtggcg cccttgtggg actccgggta ctccgtgggc tcctggcaga
    11101 gcacgcacgc ggcgaacggg ccctcggccg cggcgtcggg gccgaggccc agggcgcgca
    11161 cggccgcggc cgtgtccgtg cccggagcgg cgcagtaact ctcgtcgtcg gccatacgac
    11221 caggttaacg ccgcgccccc gccgagccga tctgctccga gacgcggacg aagcggtacc
    11281 cgcg
//
