LOCUS       NZ_CP060742            10327 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces alfalfae strain XN-04 chromosome, complete genome.
ACCESSION   NZ_CP060742
VERSION     NZ_CP060742.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     The Biocontrol and Plant Growth-promoting Properties of Streptomyces
            alfalfa XN-04 Revealed by Functional and Genomic Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2020) College of Plant Protection, Northwest A&F
            University, No. 3 Taicheng Road, Yangling 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141485.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,142
            Genes (coding)                    :: 6,967
            CDSs (with protein)               :: 6,967
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 75 of 175
            Pseudo Genes (incomplete)         :: 141 of 175
            Pseudo Genes (internal stop)      :: 22 of 175
            Pseudo Genes (multiple problems)  :: 59 of 175
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:18:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4571076
            Orig. end                         :: 4581403
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10327
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="16"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10327
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10327
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5327](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5327
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(820..1995)
                     /locus_tag="H9W91_RS20620"
                     /old_locus_tag="H9W91_20610"
     CDS             complement(820..1995)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644663.1"
                     /locus_tag="H9W91_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20610"
                     /product="glycosyltransferase"
                     /protein_id="WP_198503260.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.9e-34,
                     bitscore: 116.4, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHSPGPRILHVSRPVEGGVAQVVTDLARAQAATGADVHVACPDE
                     GMLPRVWDTADGGVRGHPWTASRSPGPQLAAEVRDLARVIARVRPALVHAHSAKAGLAA
                     RLALRGRVPTVFQPHAWSFEAVGGVTAPLTRCWERRAARWTDRIVCVSEAEWITGRRAG
                     VTGAYTVIPNGVDVARFAPGRRSEARRRLLPDSFARAPLVVCVGRLCRQKGQDVLLRAW
                     AAVAEALPGARLVLVGDGPDAAGLRDLADTTVTFAGATDDTACWYRAADVVVLPSRWEG
                     MALAPLEAMACGRPVVVTDVDGARESLPPADRAHALVPPSDPAALARALLALLRDGPLR
                     AELGRRGRRHVLDHHDARRTASAIDAVYRELLGPAPTTYPATAKYRECTTT"
     misc_feature    2020..2035
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.24"
                     /tool="antismash"
     misc_feature    complement(2077..2091)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.14"
                     /tool="antismash"
     gene            2382..2621
                     /locus_tag="H9W91_RS20625"
                     /old_locus_tag="H9W91_20615"
     CDS             2382..2621
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="H9W91_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20615"
                     /product="chaplin"
                     /protein_id="WP_062774708.1"
                     /transl_table=11
                     /translation="MSRTTKVLAFSAVAAAAVAGSAGAASADADAKGTASNSPGVVSGN
                     VVQVPVNVPVNVCGNTVNIVGLLNPAFGNKCVNH"
     gene            2882..3460
                     /locus_tag="H9W91_RS20630"
                     /old_locus_tag="H9W91_20620"
     CDS             2882..3460
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H9W91_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H9W91_20620"
                     /product="hypothetical protein"
                     /protein_id="WP_062774710.1"
                     /transl_table=11
                     /translation="MRALPVRRIATTTLCATLLLGTAGPALASADSASREETRTAPRAP
                     VMTPKAMLAEAKRLQDAGGVITPAADLLVAVLEAEGYRLSAAEAAKHAQAVRTEARKVS
                     ARQPAAPAAAPAARAPEPPAGSLPALQKTVDNMLKATTTGDATGVVREGRSLVTGLLQG
                     VLGGLGGAAQPAPAPAAAPAAGPQQTPAT"
     gene            3649..3927
                     /locus_tag="H9W91_RS20635"
                     /old_locus_tag="H9W91_20625"
     CDS             3649..3927
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644659.1"
                     /locus_tag="H9W91_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20625"
                     /product="hypothetical protein"
                     /protein_id="WP_062774713.1"
                     /transl_table=11
                     /translation="MKAMKAAAVLAGSLAIAGSAAVPAFAGAQAPSGPNGGLDDLASKG
                     LEAATPVGTNMLNPKNSDSVANTVKGVVRGLSSEGPGKLIGGLPAGK"
     gene            4231..4893
                     /locus_tag="H9W91_RS20640"
                     /old_locus_tag="H9W91_20630"
     CDS             4231..4893
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473218.1"
                     /locus_tag="H9W91_RS20640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20630"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_211826862.1"
                     /transl_table=11
                     /translation="MSATELPLRLPAQSAGGRGPAPAPPAEGPGRWFGVLLVVTGAAGL
                     LAAWVITLDKFKLLEDPDFRPGCSLNPVVACGSIMRSEQAAVFGFPNPLLGLVAYAVVA
                     GVGASVLAGARFPRWYWLALNAGMLFGVAFCTWLQFQSLYRVNALCLWCCLAWAATILM
                     FWSVTAHNVRRGFLPAPRGLRTVVAECAWVPPALHMGVIGMLVLTRWWDFWTSGTAG"
     misc_feature    complement(4921..4935)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.19"
                     /tool="antismash"
     gene            5001..5327
                     /locus_tag="H9W91_RS20645"
                     /old_locus_tag="H9W91_20635"
     CDS             5001..5327
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="H9W91_RS20645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20635"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_079250378.1"
                     /sec_met_domain="melC (E-value: 3.8e-37, bitscore: 126.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTPWVTREQSPAPAQAPAEVPDGPPAGFDEMYRGRRIQGEPVAV
                     REGRTPESWRITVDGRPLGLMRRADGSFLSMVDHYQSYDTPLAAARGAVDEIGPLQTLS
                     GRHH"
     gene            5365..6237
                     /locus_tag="H9W91_RS20650"
                     /old_locus_tag="H9W91_20640"
     CDS             5365..6237
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="H9W91_RS20650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20640"
                     /product="tyrosinase family protein"
                     /protein_id="WP_076685203.1"
                     /transl_table=11
                     /translation="MAPTRKNQSRLTKAERRRFVDAVLKLKRRGEYDAFVRTHLDFYVS
                     DGESRLRVAHMAPSFFPWHRRFLLDFERALQRIDAGVTVPYWDWTKDRTPAASLWGDDL
                     MGGTGRGPDRRVTTGPFARGSGHWVIKVGVTDGDFLTRDLGRPRAPIALPTAREVADAM
                     ADPRYDVPPYDSTARAGFRNRLEGWTSARGDARWRNHNRVHRWVGGHMLGGASVNDPVF
                     WLNHAFVDLLWDRWRRRHPRSAPYLPAEPPGAGSSQYRRVVARHERMPPWDVRPDDLLD
                     HRAHYRYGG"
     gene            complement(6333..6608)
                     /locus_tag="H9W91_RS20655"
                     /old_locus_tag="H9W91_20645"
     CDS             complement(6333..6608)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116479.1"
                     /locus_tag="H9W91_RS20655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20645"
                     /product="chaplin"
                     /protein_id="WP_062774722.1"
                     /transl_table=11
                     /translation="MSRIAKVTAATLGTSAVVLGGAGMAMADADAKGAAVGSPGAISGN
                     VVQVPVHAPINLCGNTVNVVGLLNPAFGNECTNVSKHEHGHGGYGR"
     misc_feature    complement(6742..6755)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.23"
                     /tool="antismash"
     gene            complement(6812..7072)
                     /locus_tag="H9W91_RS20660"
                     /old_locus_tag="H9W91_20650"
     CDS             complement(6812..7072)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H9W91_RS20660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H9W91_20650"
                     /product="hypothetical protein"
                     /protein_id="WP_123985604.1"
                     /transl_table=11
                     /translation="MSAHLTRPLLIFALALVPPLGIVSAAATPTGPSPAVTTTSESSKS
                     ARDASVRDASVHDTSVRDASVGSSPACDWRYGSSCVVGGRM"
     gene            7269..8483
                     /locus_tag="H9W91_RS20665"
                     /old_locus_tag="H9W91_20655"
     CDS             7269..8483
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501751.1"
                     /locus_tag="H9W91_RS20665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20655"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_076685201.1"
                     /transl_table=11
                     /translation="MKIDCVGGGPASLYLAILAKLKDPAHEVTVHERHAEGASHGWGVT
                     FWPDLLEELRAHDPESAAVIAERCVSWRGGFAHVGDRTTAHDGDLGHAIGRHEMRTILA
                     DRARSLGVRLAFGTEIGGRGDLPDADLVVAGDGAHSRLRTAHAEHFGTRVTTGANRFIW
                     LGTTRVFTAFTFAFVETEHGWIWCYAYGYSGGHSTCVVECSAATWTGLGFDRIAHTDAL
                     RLLEKLFAEPLDGHGLLGRDDIEGHAQWQAFPTVTNATWSHGNLTLIGDAAHTTHYSIG
                     AGTTLALRDAACLAGELRGVGPRELPDALARYERRRKHALLAVQSAARHSAQWYENLPR
                     YIGMPPERMFALLGQRHSPLLPYVPPQIYYHLDQTVGRLNSLRWLKQRLGPRVARALQG
                     RRPES"
     gene            8749..9669
                     /locus_tag="H9W91_RS20670"
                     /old_locus_tag="H9W91_20660"
     CDS             8749..9669
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935403.1"
                     /locus_tag="H9W91_RS20670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_20660"
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="WP_211826863.1"
                     /transl_table=11
                     /translation="MTQGEAFTNPDALKGLTAYDRTGEKIGSIERVYLDDRSGRPEWVT
                     VKTGLFGMKESFVPLSGARLQQDALHVSCTKEAVKEAPRVDADQHLEPGQEHELYAHYG
                     LSRPGMGTTGTGTGTGMGAAGTAAGTGTGMAAGERPMAGAAGGREYAMRGKERDDRAQD
                     MIRSEERLRVGTEEEEVGHAHLRKTVVNEDVTTSVPVTHEEVRVVREPIREGDRSRADI
                     GEAETEVTLHAEHAVVSKESVPVERVHLETERVTETEEVTGTVRKEQIEFDDGTKDSRR
                     RGMKGDRRGTKGTDAWREGKQGPNH"
ORIGIN
        1 ggtcgatctc ccagaggtcg cagccgaggc cgcgcagggc ggacagccgg tcggcgggtc
       61 cgccgtggcc cgcgagcagc aggacggcgc gcacgtcgtt ctggatgacg gcccggtgca
      121 gttcgtcctc cgtgcgcagc accggcagtc cgggcgcggc ggacggctcg gcgccgtcct
      181 cgtcggcggg ccgctgctcc gccgcctcgt ccacgacgcc gacggggcgc agcccgcagc
      241 ggggccgccg cagcagggcc gcggccacgg cctgggcctg cccgccgggg ccgagcacca
      301 ggaccggccg tggtcttcgc agggcgcggg cccgcagccg ccggtggacc agggcgcgtc
      361 cgcagacgcc gatcaggcac tgcgtggcgg ccatcatgag cagcgtgcgc ggggcgagct
      421 gcggcatgtg cgcgggcagg gcgggcgcga gcgccgcgac gagggcccag ccgagcgcga
      481 cccggccgca gagcgagggc acctcgtcca gcgtggcggg cacggcggtg tacgcgtaca
      541 ggcggccccg ggcgttcagg gcgaggacgc cgacgacgag caggggggcg agcacggcga
      601 tgcggcgggg atcggccagc aggaacatgc cgagcagcgc ggcggcggcg tccacggtga
      661 ccagggcggc ggccgtgcgc cgcccccgcg cgggccccct cgccggggcg ccggcgcgcg
      721 ggccgagggc gccgcggggc gccacgacga ggggtccggc cggggggtac gtgtcctgcc
      781 gcggctgtcc cgcggaggag gcgacggttc gttcggcact cacgtggtcg tgcactccct
      841 gtacttggcg gtcgccgggt acgtggtggg cgccgggccc agcagctccc ggtacaccgc
      901 gtcgatcgcc gacgcggtcc gccgggcgtc gtggtgatcg aggacatggc ggcggccccg
      961 ccgcccgagt tcggcccgca gcgggccgtc ccgcagcagg gcgagcagcg cccgtgccag
     1021 cgccgccggg tcgctgggcg gtacgagggc gtgcgcgcgg tcggccgggg gaaggctctc
     1081 cctggctccg tccacgtcgg tgaccaccac gggacggccg caggccatgg cctccagcgg
     1141 ggccagggcc atgccctccc agcgggaggg caggacgacc acgtcggccg cccggtacca
     1201 gcaggcggtg tcgtccgtcg cccccgcgaa cgtcacggtc gtgtccgcca ggtcccgcag
     1261 cccggcggcg tcggggccgt cgccgacgag gacgagccgg gccccgggca gcgcttcggc
     1321 gacggcggcc caggcgcgga gcaggacgtc ctgccccttc tgccggcaca gccggcccac
     1381 gcacaccacg aggggggccc gcgcgaagga gtcgggcagc agccggcgac gggcctcgga
     1441 ccggcggccg ggggcgaagc gggcgacatc gacgccgttg gggatcaccg tgtacgcacc
     1501 cgtgacgccc gccctgcggc cggtgatcca ctcggcctcg ctgacgcaga cgatgcggtc
     1561 cgtccagcgg gcggcgcgcc gctcccagca gcgggtcagg ggcgcggtga cgccgccgac
     1621 cgcctcgaag gaccaggcgt gcggctggaa gacggtcggg acgcgcccgc gcagggcgag
     1681 gcgcgcggcg aggcccgcct tggcgctgtg ggcgtgcacg agcgccggcc gcacgcgggc
     1741 gatgacgcgg gccaggtccc gtacctctgc ggcgagttgt gggccggggg agcgcgacgc
     1801 cgtccacgga tggccgcgta cgccgccgtc ggcggtgtcc cagacccggg gcagcatgcc
     1861 ctcgtcgggg caggccacgt ggacgtcggc gcccgtcgcg gcctgggcgc gggccaggtc
     1921 ggtgaccacc tgggccacac cgccctcgac gggccggctg acgtgcagga tccgcgggcc
     1981 cggggagtga cgcatgacgg cgttggcctt ccgacgtgaa gagctctgaa ccacagcgga
     2041 gataaggcat ctgcaaggaa tgccgggatc tgacgtatat gactgaaata tcccaatgcg
     2101 atgcgcaact gtagctcggt ggaggagtag ctccgccgat cccggcgcgt gtgtcggatc
     2161 ggtgaaatgc gtggtggggc ggggtcgatg ggaacggcac acgtatccgg tgcgcccagc
     2221 gggccgccgg tcgcggcgtc ggccggtgcc ctcgccgtca gcggggcgca cggcacacat
     2281 cactcttctg ggtgcacaac tcggcgtccg gatcggcagt tgatttcccg tacacgggca
     2341 ttccgcccgt gcgtcacacc tttgccaagg agcagcaccc catgtcccgt accaccaagg
     2401 tcctcgcctt ctccgccgtc gccgccgccg ctgtggccgg atcggcgggt gccgcctccg
     2461 cggacgccga cgcgaagggc accgcctcca actcacccgg cgtggtctcc ggcaacgtcg
     2521 tacaggtgcc ggtcaacgtc cccgtgaacg tctgcggcaa caccgtgaac atcgtcggcc
     2581 tgctgaaccc cgcgttcggc aacaagtgcg tcaaccactg acacagcgcc cggagaacgg
     2641 ccgtccccgc ggaggcgggg gcggccgttc ccgcgttcgg agcagtcgcc gggggcccgt
     2701 gccccgaatg gagtggcgtc aggaaattat ccgaccgtcc gtcaaatctc gtccccacct
     2761 gcgctgatgc tcccggacgc ccggtggagc gccgggccgg gtggaccgct cggttgcggt
     2821 gggcccgcac cgctcgcacg gtgatcgcaa gatccgacgt atcaccgaaa ggctgtgacc
     2881 tatgcgcgct ctgcccgtac ggcgtatcgc gaccaccacc ctgtgcgcga cgctgctcct
     2941 cggcacggcc ggccccgcgc tcgcctccgc ggactccgct tcccgcgagg agaccaggac
     3001 cgcgccccgc gcacccgtca tgacgccgaa ggccatgctg gccgaggcga agcggctgca
     3061 ggacgccggc ggtgtcatca ccccggccgc cgacctgctg gtggcggtgc tggaggccga
     3121 ggggtacagg ctctccgccg cggaggccgc gaagcacgcc caggccgtac ggacggaggc
     3181 ccggaaggtg tccgccaggc agcccgccgc tccggccgcc gccccggccg ccagggcccc
     3241 cgagcccccg gcgggcagcc tccccgccct gcagaagacg gtcgacaaca tgctcaaggc
     3301 caccacgacc ggtgacgcca ccggcgtcgt cagggagggg cgctcgctcg tcacggggct
     3361 gctccagggc gtgctcggcg gcctcggcgg cgcggcccag ccggccccgg cgcccgctgc
     3421 ggccccggcc gccggaccgc agcagacgcc cgcgacctga gcggcggacg gcgggcggcc
     3481 ttcatgcgcg cgggtccgcg ggaagttccc ggtccagggg tttccgtacc ggcccgtgct
     3541 cgttggttcc ttacgaagtg acacgacagg tgaagtgaca cgtccgggcg atcgacgcca
     3601 gggcggcacg tccaccgggc gacacgcgtt gccagagaaa ggaacaagat gaaggccatg
     3661 aaggctgccg ccgtccttgc cggttccctc gccatcgcgg gttccgccgc cgtgcccgcg
     3721 ttcgccggcg cccaggcgcc ctccggcccg aacggcggcc tcgacgacct cgcgagcaag
     3781 ggtctggagg ccgccacccc ggtgggcacg aacatgctca accccaagaa cagcgactcc
     3841 gtggccaaca cggtcaaggg cgtcgtcagg ggcctgagct ccgaaggccc cggcaagctc
     3901 atcggcggcc tccccgccgg caagtagccg cgcctcgcgc agcccccccg ggaacacaga
     3961 gggccggctc ccttcgacgg gccggccctc cggcgtgcgc acgggcggtc aaggcgtccc
     4021 gtacggccac cagttcgacc cggagccgtc ctgagcccca ccggtgtgac cccatcgtgt
     4081 gatggctgcc cgcggctccg ccacccgggt gacaacggag ccggtcgcgc cgccgggcgt
     4141 ctatacggtc tcccccatga ccacgaccac gacttccggc ggcccccgcg acgccgcgca
     4201 ccgcccgccg ccgacgcccg gccccggccg atgagcgcca cggaactgcc gctccggctg
     4261 ccggcgcagt ccgcgggcgg gcgcggcccg gccccggcgc cgcccgccga ggggcccggc
     4321 cggtggttcg gcgtgctgct cgtggtcacc ggggcggcgg ggctgctcgc ggcgtgggtc
     4381 atcacgctcg acaagttcaa gctgctcgaa gacccggact tccggccggg gtgcagcctc
     4441 aacccggtcg tggcctgcgg cagcatcatg cggagcgagc aggcggcggt cttcgggttc
     4501 cccaacccgc tcctcggcct cgtcgcctac gccgtcgtgg ccggcgtggg cgcgagcgtg
     4561 ctggcgggcg cccgcttccc ccgctggtac tggctggcgc tcaacgcggg catgctcttc
     4621 ggcgtcgcct tctgcacctg gctccagttc cagtcgctct accgcgtcaa cgcgctctgc
     4681 ctgtggtgct gcctggcctg ggccgcgacc atcctcatgt tctggtccgt gacggcgcac
     4741 aacgtccggc gcggattcct ccccgcgccc cgggggctgc gtaccgtcgt cgcggaatgc
     4801 gcctgggtcc cgcccgccct gcacatgggc gtcatcggca tgctcgtcct cacgcgctgg
     4861 tgggacttct ggacgagcgg aacggcgggg tgagccggcc gtggggcacg cacggtgagc
     4921 cggaacggct cgtcgaccgg gtgacgcgcc gtcacctcct cggcgtcgcg gcctcggccc
     4981 tgctcgccgc cgccgagatc atgcggacgc cctgggtcac gcgcgaacag agtccggccc
     5041 cggcgcaggc cccggccgag gtgccggacg gcccgcccgc cggcttcgac gagatgtacc
     5101 ggggccgccg catccagggg gagccggtgg ccgtgcgcga gggacgtacg ccggagagct
     5161 ggcggatcac cgtggacggc cgtccgctgg gcctcatgcg ccgcgccgac ggcagcttcc
     5221 tcagcatggt cgaccactac cagtcgtacg acaccccgct ggccgccgca cgaggcgccg
     5281 tggacgagat cggccccctg caaaccctga gcggccggca ccactgagtc cggcggccca
     5341 ccccggaacg cggaacggac ggacatggcc cccacgcgca agaaccagag caggctgacc
     5401 aaggccgagc ggcgcaggtt cgtcgacgcg gtgctgaagc tgaagcgccg gggcgagtac
     5461 gacgccttcg tgcgcacgca cctcgacttc tacgtctcgg acggcgagag ccgcctgcgg
     5521 gtcgcgcaca tggccccctc gttcttcccg tggcaccgcc gcttcctgct ggacttcgag
     5581 cgggcgctcc agcggatcga cgcgggcgtg accgtcccgt actgggactg gacgaaggac
     5641 cggaccccgg cggcgtccct gtggggcgac gacctgatgg gcggcacggg acgggggccg
     5701 gaccgccggg tcacgacggg gccgttcgcc cgggggagcg gccactgggt catcaaggtg
     5761 ggcgtcaccg acggggactt cctcactcgc gacctggggc gtccgcgcgc ccccatcgcc
     5821 ctgccgaccg cgcgggaggt ggccgacgcg atggcggacc cgcgctacga cgtcccgccc
     5881 tacgactcca cggcacgcgc gggtttccgc aaccggctgg agggctggac gtcggcgcgg
     5941 ggcgacgcgc ggtggcgcaa ccacaaccgg gtccaccgct gggtcggcgg gcacatgctc
     6001 ggcggggcct ccgtcaacga cccggtcttc tggctgaacc acgccttcgt cgacctgctg
     6061 tgggaccgct ggcgccgccg ccatccccgc tcggccccct acctgcccgc ggagccgccg
     6121 ggcgccggct cgtcccagta ccgccgcgtc gtcgcgcgcc acgagcggat gccgccgtgg
     6181 gacgtgcgcc cggacgacct cctcgaccac cgcgcgcact accggtacgg ggggtagccc
     6241 ccggcgggcc gttcaccggg ggccggtgga ccgctcaccg agggccggcg ggccggcggg
     6301 ccggcgggcc gctcggggcg gcccgggggc actcagcggc cgtagccgcc gtggccgtgc
     6361 tcgtgcttgc tgacgttcgt gcactcgttg ccgaaggcgg ggttcagcag gccgacgacg
     6421 ttgacggtgt tgccgcagag gttgatcggg gcgtggaccg gaacctgcac gacgttgccc
     6481 gagatggcgc cgggggaacc gacggcagcg cccttcgcgt ccgcgtccgc catggccatg
     6541 cccgcgccac cgagtacgac ggcactggta ccgagggtgg cggcggtgac cttcgcgatg
     6601 cgagacatca cgttctcctt tgacttggtg agcgcggctg cctacgcgca gccgcaccct
     6661 cgttaacgcc ccgcgtcaac gtgaagtcac ggcaaggagt gccgcgaccg ccatgaacaa
     6721 ggacatttcg cagatgagat ggcacgcaga gtggtcgcgt ggaggagagg gacgtcgcgt
     6781 ggtggttcgg cgccgctcgc cccgcgcggt gtcacatgcg cccgccgacg acgcacgagc
     6841 tgccgtaccg ccagtcgcag gcgggcgaac tcccgacgga ggcgtcccgc acagaggtgt
     6901 cgtggacgga ggcatcccgt acggaggcgt cccttgcgga cttgctgctc tcggaggtgg
     6961 tggtcaccgc cggggagggc cccgtcggcg tggccgccgc ggacacgatc cccagcgggg
     7021 ggaccagggc cagcgcgaag atcaggagcg gcctggtgag gtgagccgac acgtccggtc
     7081 tcctcgtgat cgacgtaacc cgagacagag tgatggtgca cacatctcac ccaagatcgc
     7141 atgccgatca ctttcggtga gcggcggtgg ggtgacgtgc ggcgacactg ccgcgccctg
     7201 ggcggcggag acgccacggg cggctacttt atgtcaccgt tccgcttccc tcggcgacag
     7261 gagtccccgt gaagatcgat tgcgtcggcg gaggccccgc atccctgtat ctggccatcc
     7321 tggcgaagct caaggacccg gcccacgagg tcaccgtcca tgagcggcac gccgagggcg
     7381 caagccacgg ctggggcgtg accttctggc cggacctcct ggaggagctg cgggcgcacg
     7441 accccgagtc ggccgcggtc atcgccgagc ggtgcgtgag ctggcgcggc ggcttcgccc
     7501 acgtcggcga caggaccacc gcgcacgacg gcgatctggg ccatgccatc ggacgccacg
     7561 agatgcggac gatcctcgcg gaccgggccc gctccctcgg cgtgcgcctg gcgttcggca
     7621 ccgagatcgg cggtcgcggc gacctgccgg acgcggacct ggtcgtcgcg ggcgacggcg
     7681 cgcacagcag gctccgcacg gcgcacgcgg agcacttcgg cacccgggtg accaccggcg
     7741 ccaaccgctt catctggctc ggcacgaccc gcgtcttcac ggccttcacc ttcgcgttcg
     7801 tggagaccga acacggctgg atctggtgtt acgcgtacgg gtacagcggc gggcacagca
     7861 cctgcgtggt ggagtgctcg gcggccacct ggaccggcct gggcttcgac cggatcgccc
     7921 acaccgacgc cctgcggctg ctggagaagc tgttcgccga gccactggac ggccacgggc
     7981 tgctcggccg cgacgacatc gagggccacg cgcagtggca ggcgttcccg acggtcacca
     8041 acgccacctg gtcccacggc aacctgacgc tgatcggcga cgccgcccac accacgcact
     8101 actccatcgg cgcgggcacc acgctggcgc tccgcgacgc cgcgtgcctg gcgggggagc
     8161 tgaggggcgt gggcccgagg gagctgcccg acgccctcgc gcgctacgag cgccgccgca
     8221 agcacgccct gctcgcggtg cagagcgcgg cccggcacag cgcccagtgg tacgagaacc
     8281 tgccccgcta catcggcatg cccccggagc ggatgttcgc gctcctcggc cagcgccact
     8341 cgccgctgct gccctatgtg ccgccgcaga tctactacca cctggaccag acggtcggcc
     8401 gactcaactc cctccgctgg ctgaagcagc ggctggggcc ccgcgtggcc agggcgctgc
     8461 agggccggcg gccggagtcc tgacgggcgg agtcccgacg gccggtggcc catgaatgca
     8521 aaccccaagt gttcacccgt tcggaccctc ggggccgaaa ccgctgtgac cggctcgatc
     8581 gcctcggttg ccggggcgga gaacgcgggc gagccgttcc ccgcacgctc cgtgactccc
     8641 cgccttgcgg ggcgggccgg ggcggcgcgc gatggacctt aagaggcgtg cgtcgttccg
     8701 tggcggatcg gatttcgccc gtcgcatgct caaagggagt gatcacccat gacgcagggc
     8761 gaggccttca ccaaccctga cgcgctcaag ggcctgacag cctatgaccg gaccggcgag
     8821 aagataggca gcatcgaacg ggtctacctc gacgaccgca gcggccgccc cgagtgggtg
     8881 accgtcaaga cgggcctctt cggtatgaag gagagcttcg tgccgctgtc cggggcgcgc
     8941 ctccagcagg acgccctgca cgtctcgtgc accaaggagg ccgtgaagga ggcgccgcga
     9001 gtcgacgccg accagcacct ggagcccggt caggagcacg agctgtacgc gcactacggc
     9061 ctgtcccgcc ccggcatggg caccactggc accggtacgg gcacgggcat gggcgccgcc
     9121 ggtacggccg ccggtacggg caccggcatg gccgcgggcg agcgcccgat ggccggagcc
     9181 gccggtggcc gcgagtacgc catgcgcggc aaggagcgcg acgacagggc gcaggacatg
     9241 atccgctccg aggagcggct gcgggtcggc accgaggagg aagaggtcgg ccacgcccac
     9301 ctccgcaaga cggtggtgaa cgaggacgtc acgacgtccg tgccggtcac gcacgaggag
     9361 gtccgcgtcg tccgcgagcc gatccgcgag ggcgaccgct cgcgcgccga catcggcgag
     9421 gcggagacgg aggtgaccct ccacgccgag cacgcggtgg tgagcaagga gtccgtgccg
     9481 gtggagcgcg tccacctgga gaccgagcgg gtcacggaga ccgaggaggt caccggcacc
     9541 gtccgcaagg agcagatcga gttcgacgac ggcacgaagg acagccgccg ccgcggcatg
     9601 aagggcgacc gccggggcac gaagggcacc gacgcctggc gcgagggcaa gcagggcccg
     9661 aaccactgac ccgcgcccag ggccaccggc cccccggcag aggccctcac ccaccgggtg
     9721 agggccttcg ccatgtccga ccggggccgg gccgcgccgc cgcctgcccc tgctgcctcg
     9781 ccgcgccacg cgtcgtgtcc gatgagcggc cgatgaccac gccccatcgg gctcagacga
     9841 tcgacttgat ggtcctgtcc gcgtaccgca gcgtctcgtc gatggtcctg cccatctcac
     9901 gcacggcgtc gggccgtgcc gtcaggaggg cgctgacgtt gatgctgctc acagcgagga
     9961 ggcaaccggc cacggtcagg aactcccggt accgctcctg cccctcatgc ccgagcgatg
    10021 cccagtgccg gggctccggc ggcagattca ccctccactt ctcgagggcg tgggtgccgt
    10081 ggacgctgac gtactccatg atctccgtgg cgcgatgcag tacgtcgatc atcgcgtcga
    10141 agccccgctg cgactggtcc agagccgcct ccgcggcttc gacctcggtt cggagacgct
    10201 cctcctcctt cttgttccga cgctgcctca cgacgtagaa acctgcggcg gccagcgctg
    10261 ccaccggggc ggcgaccatg ccggtgagca ggagggtccc accggccatc cccgcaccgc
    10321 cggcggc
//
