LOCUS       NZ_CP060742            10405 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces alfalfae strain XN-04 chromosome, complete genome.
ACCESSION   NZ_CP060742
VERSION     NZ_CP060742.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     The Biocontrol and Plant Growth-promoting Properties of Streptomyces
            alfalfa XN-04 Revealed by Functional and Genomic Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2020) College of Plant Protection, Northwest A&F
            University, No. 3 Taicheng Road, Yangling 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141485.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,142
            Genes (coding)                    :: 6,967
            CDSs (with protein)               :: 6,967
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 75 of 175
            Pseudo Genes (incomplete)         :: 141 of 175
            Pseudo Genes (internal stop)      :: 22 of 175
            Pseudo Genes (multiple problems)  :: 59 of 175
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:18:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2152253
            Orig. end                         :: 2162658
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="13"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            846..1946
                     /locus_tag="H9W91_RS09685"
                     /old_locus_tag="H9W91_09675"
     CDS             846..1946
                     /NRPS_PKS="Domain: Aminotran_5 (17-296). E-value: 3.1e-29.
                     Score: 94.0. Matches aSDomain:
                     nrpspksdomains_H9W91_RS09685_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644018.1"
                     /locus_tag="H9W91_RS09685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09675"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_245001718.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.1e-22, bitscore:
                     78.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAQHFAAIEARVARLLGTGQDVVIMQGEALLPLE
                     GCIRSAARPGTTALNVITGPYGQTFGNWLRDCGADVVDLAVPFHTAVTAEQVRRALAER
                     PEIDFVSLVHAEAATGNTNPVAEIGAVVREHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRIAANPRAPRRSYLSLLDWKERWIDAGRKALLHAP
                     AQLEMLALEACVERIETEGLDALMARHATAAAATRAGALALGGGLRPYVHEAADAAPVA
                     TTLRAPEGVDASDLVAAALAADPVLPLVAGGGALAQEMIRVNHYGPDATRGVVHASLAA
                     LGAALGEAGVAVDLEGARRAVTDAWS"
     aSDomain        897..1733
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS09685_Aminotran_5.1"
                     /evalue="3.10E-29"
                     /label="H9W91_RS09685_Aminotran_5.1"
                     /locus_tag="H9W91_RS09685"
                     /protein_end="296"
                     /protein_start="17"
                     /score="94.0"
                     /tool="antismash"
                     /translation="AIEARVARLLGTGQDVVIMQGEALLPLEGCIRSAARPGTTALNVI
                     TGPYGQTFGNWLRDCGADVVDLAVPFHTAVTAEQVRRALAERPEIDFVSLVHAEAATGN
                     TNPVAEIGAVVREHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVS
                     VSERAWQRIAANPRAPRRSYLSLLDWKERWIDAGRKALLHAPAQLEMLALEACVERIET
                     EGLDALMARHATAAAATRAGALALGGGLRPYVHEAADAAPVATTLRAPEGVDASDLV"
     gene            2031..2558
                     /locus_tag="H9W91_RS09690"
                     /old_locus_tag="H9W91_09680"
     CDS             2031..2558
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309984.1"
                     /locus_tag="H9W91_RS09690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09680"
                     /product="DinB family protein"
                     /protein_id="WP_198502309.1"
                     /transl_table=11
                     /translation="MTTLPDGRRIPPLTADEPAMLAGWLDLHRDTLAVKCAGLDDEQTL
                     RACVEPSALSLFGLVQHLAEVERNWIQRVFAGRDVPLLYGMPPGGFAVDGSRTFAEVLA
                     VWEREVAVSREICAGRDLDETGRLGPEQRVVTGGEAVVSLRWILVHLIEEYARHNGHAD
                     LIRERIDGVTGT"
     misc_feature    2704..2720
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.98"
                     /tool="antismash"
     misc_feature    complement(2707..2723)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.98"
                     /tool="antismash"
     misc_feature    2883..2898
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3031..3045
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.38"
                     /tool="antismash"
     gene            3054..3605
                     /gene="ectA"
                     /locus_tag="H9W91_RS09695"
                     /old_locus_tag="H9W91_09685"
     CDS             3054..3605
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="H9W91_RS09695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09685"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_076686877.1"
                     /transl_table=11
                     /translation="MTAAQADLPTDLQAKFLEMPEGLRIDSPAVADGAAIWRIARDSKV
                     LDLNSSYSYLLWCRDFAATSVVARGADGEPVGFITGYFRPERPDTLLVWQVAVDHAQRG
                     RGLAGALLDGLTAKVTAGRAPITVETTITPGNTASERLFLSYARRHGAAVEQKILFDAG
                     LFPDDGHEPEVLYRIGPVGR"
     misc_feature    complement(3096..3110)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.75"
                     /tool="antismash"
     gene            3676..4947
                     /gene="ectB"
                     /locus_tag="H9W91_RS09700"
                     /old_locus_tag="H9W91_09690"
     CDS             3676..4947
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 2.1e-72.
                     Score: 235.9. Matches aSDomain:
                     nrpspksdomains_H9W91_RS09700_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="H9W91_RS09700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09690"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_076686876.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.5e-77, bitscore:
                     257.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGIAHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGRTPDFIWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     EWLRKLADVCERWDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALEAYWTDGSAMEKQTRARGEQVEQVL
                     ASIVEENRADIKEFRGRGLVWGIEFLDKDRASAVAKRAFELGLLIETSGPEGEVVKLLP
                     ALTITPEELDEGLRTLARAVRETA"
     aSDomain        3760..4770
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS09700_Aminotran_3.1"
                     /evalue="2.10E-72"
                     /label="H9W91_RS09700_Aminotran_3.1"
                     /locus_tag="H9W91_RS09700"
                     /protein_end="365"
                     /protein_start="28"
                     /score="235.9"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGIAHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGRTPDFIWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARAEWLRKLADVCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALEAYWTDGSAMEKQTRARGEQVEQVLASIVEENRADIKEFRGRGLVWGIEF"
     gene            5001..5405
                     /locus_tag="H9W91_RS09705"
                     /old_locus_tag="H9W91_09695"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536875.1"
                     /locus_tag="H9W91_RS09705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09695"
                     /product="ectoine synthase"
                     /protein_id="WP_062771849.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-52, bitscore:
                     175.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDDTTGEKYTITPGTMYLLDGHEKHTMRIKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPDEA"
     gene            5480..6379
                     /gene="thpD"
                     /locus_tag="H9W91_RS09710"
                     /old_locus_tag="H9W91_09700"
     CDS             5480..6379
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848585.1"
                     /locus_tag="H9W91_RS09710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09700"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_062771846.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-38, bitscore: 132.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTTDLYPTRGAEEVLIERKDPVVWSEPGAEGPISAGDLA
                     GFERDGFLAIDQLITPDEVSVYHAELERLVNDPAMRADERSIVEPRSQEIRSLFEVHKI
                     SELFGKLAADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTKNHDTNGGLMIMPGSHKTFLGCAGATPKDNYKRSLQMQDAGIPSNET
                     LTHLASDHGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(6460..7521)
                     /locus_tag="H9W91_RS09715"
                     /old_locus_tag="H9W91_09705"
     CDS             complement(6460..7521)
                     /NRPS_PKS="Domain: Aminotran_5 (125-347). E-value: 1.1e-22.
                     Score: 72.4. Matches aSDomain:
                     nrpspksdomains_H9W91_RS09715_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156955.1"
                     /locus_tag="H9W91_RS09715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09705"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_076686875.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 3.9e-07,
                     bitscore: 28.3, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.1e-17, bitscore:
                     61.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLGIDHHVSAEFAPETTFLNTAVRGLLPRRAAEAVQALTAELV
                     SGGRSGSGDFATVDRARASFARLVGVDPSRVATGGSVAVHVGLIAASLPAGAEVLFPEG
                     DFSSLINPFVLRGDLKPRFAPLDELADAIRPKTALVAFSTVQSADGRTADCAAIREAAA
                     AHGARTLADTTQSAGWQPLDAGAYDYSVTGAFKWLLCPRGTSFLTVGEAAQESLAPLHG
                     GWLAAADAWNSTYGPIGEVVADARRFDEPPAFLAHHGAAAALSLIEEIGVDAIHRHDLA
                     LAARYRAGLAGLGHAPVPGASPIVSVPGLADREPELARAGVVVAARAGHLRATFHLYNT
                     EADVDRLLDVLAG"
     aSDomain        complement(6481..7146)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS09715_Aminotran_5.1"
                     /evalue="1.10E-22"
                     /label="H9W91_RS09715_Aminotran_5.1"
                     /locus_tag="H9W91_RS09715"
                     /protein_end="347"
                     /protein_start="125"
                     /score="72.4"
                     /tool="antismash"
                     /translation="LDELADAIRPKTALVAFSTVQSADGRTADCAAIREAAAAHGARTL
                     ADTTQSAGWQPLDAGAYDYSVTGAFKWLLCPRGTSFLTVGEAAQESLAPLHGGWLAAAD
                     AWNSTYGPIGEVVADARRFDEPPAFLAHHGAAAALSLIEEIGVDAIHRHDLALAARYRA
                     GLAGLGHAPVPGASPIVSVPGLADREPELARAGVVVAARAGHLRATFHLYNTEADVDRL
                     "
     gene            complement(7544..8260)
                     /locus_tag="H9W91_RS09720"
                     /old_locus_tag="H9W91_09710"
     CDS             complement(7544..8260)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067467.1"
                     /locus_tag="H9W91_RS09720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09710"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_076686874.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDAVEVVHRSFELDP
                     GRAKGDTGQVLPMLMNKYGMTEEQAREGERRLGENAAAEGLDYRIEGRDHGSTFDMHRV
                     LHLAKERGRQDELIGLLYRANFAEERSVFADDERLVELAAEAGLDEAEVRAVLADPGRY
                     ADDVRADEREAAELGANGVPFFVLDRRYGVSGAQPAEVFTQALEQAWGGRSPLTTLADA
                     GDGEACGPDGCAVPGA"
     gene            complement(8375..8983)
                     /locus_tag="H9W91_RS09725"
                     /old_locus_tag="H9W91_09715"
     CDS             complement(8375..8983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="H9W91_RS09725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_09715"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_062771837.1"
                     /transl_table=11
                     /translation="MDLDLPQLPFSLRGYGPDGHWSHEDGVLTGWAGARQDRFVPPTGE
                     ALDPAADAPRLLGAPEGDFQLIARVTVGFAAPFDAGVLYLHVGEREWAKLCLERSPDEP
                     TVCTVVTRGHSDDANAFTVDGASAWLRISRTGSAFAFHASADGERWTFVRIFSLGDEES
                     AGAALVGFMAQSPVGEGCVVTYDHIEFRPNWPKGLRDGS"
     misc_feature    complement(9415..9430)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
ORIGIN
        1 gagcgggatc gtgtcctccg tgaggcccgt cgcgtgctcg acgcagtcga tgcccgcctc
       61 caccaggtcc cgcagcgagt cctccgcgaa gcagtgggcc gtcacgcggg cgccgagccg
      121 gtgggcctcc gcgatggccg cctcgaccgc gtcgcgcggc cagcaggcgg tcaggtcgcc
      181 cgcctcgcgg tcgatccagt cgccgacgag cttgacccag ccgtcgccgc gccgcgcctc
      241 ctgggcgacg tatgcgacga ggtcgtcggg ttcgatctca tgggcgtagt tgcggatgta
      301 gcggcgcgtg cgcgcgatgt gccggccggc gcggatgatc ttcggcaggt cctcgcggtc
      361 gtcgatccag cgggtgtccg acggggagcc cgcgtcgcgg atgaggagcg cgccgatctc
      421 gcggtcggtc agcgcctgct tctccgcggt ggcggcgtcc acggggccgt gcgcgtccag
      481 gcccacgtgg cagtgcgcgt cgaccaggcc gggcagggcc cacccctcga cggtggccgt
      541 ctccgtcgcc ggggtcaccg ggcggtcgta cgtcaccctg ccgccgacca cccacagttc
      601 gtcccgtacg tcctcgggcc cgacgagcac ccgccccttc acgtgcagca ccgcgtgatc
      661 actcatggca gcagcttagg aggcggcgat gacgggcggt acggggtgcc ggggaggcgg
      721 tgcgcggggt gcgggaaagg cgtgcgacgg gacgccggga acgtgccgta tgcggcggtc
      781 ggtaggctcg tgcaccgcag ccggacgaac tcgtgagcga agtgagcagt gacgtgagca
      841 gtactgtgac gcaccccttt ctggacctgg cgccgctgag cgcccagcac ttcgccgcca
      901 tcgaggcgcg ggtggcccgg ctgctcggca ccggacagga tgtcgtgatc atgcagggcg
      961 aggccctgct gcccctggag gggtgcatcc gctccgcggc gcggccgggc acgaccgcgc
     1021 tgaacgtgat cacgggaccg tacgggcaga ccttcgggaa ctggctgcgg gactgcggcg
     1081 cggacgtcgt cgacctcgcc gtgccgttcc acaccgccgt caccgccgag caggtgcgcc
     1141 gggccctcgc cgagcgcccc gagatcgact tcgtctcgct ggtgcacgcg gaggccgcca
     1201 ccggcaacac caaccccgtc gcggagatcg gcgcggtcgt gcgcgagcac ggggccctgt
     1261 tcatgctgga cgcggtcgcc tcggtgggcg ccgagccgct gctcccggac gcgtggggcg
     1321 tggacctgtg cgtgatcggc gcccagaagg cgatgggcgg cccggccggc gtctcggcgg
     1381 tgtcggtgag cgagcgggcc tggcagcgga tcgccgccaa cccgcgggcg ccgcgccgct
     1441 cctacctctc cctcctcgac tggaaggagc gctggatcga cgccgggcgc aaggccctgc
     1501 tgcacgcgcc cgcgcagctg gagatgctgg cgctcgaagc gtgcgtggag cgcatcgaga
     1561 cggagggcct cgacgccctc atggcgcggc acgccaccgc cgccgccgcg actcgcgcgg
     1621 gcgcgctcgc gctcggcggc ggtctgcggc cgtatgtgca cgaggccgcc gacgcggctc
     1681 cggtcgccac cacgctgcgc gcgcccgagg gcgtcgacgc ctcggacctg gtcgccgcgg
     1741 cgctcgccgc cgacccggtg cttccgctgg tcgccggggg cggcgccctc gcccaggaga
     1801 tgatccgggt caaccactac ggtcccgacg ccacccgcgg tgtcgtccac gcctcgctgg
     1861 ccgcgctcgg cgccgccctg ggcgaggccg gtgtcgccgt ggacctggag ggggcccgcc
     1921 gcgcggtgac ggacgcctgg agctgaccgg cgcgccgccc gcgcggcgaa ggcagaagag
     1981 cctcgggacc cccttccccc gaggcgctcc cgcatggcag cgtgccaggc atgaccacgc
     2041 tgccggacgg acgccgtatc ccgccgctga ccgccgacga gccggccatg ctggccggtt
     2101 ggctcgatct gcaccgcgac accctcgccg tgaagtgcgc ggggctggac gacgagcaga
     2161 cgctgcgcgc ctgcgtggag ccttccgcgc tctcgctgtt cgggctggtg cagcacctgg
     2221 ccgaggtcga gcggaactgg atccagcggg tgttcgccgg gcgtgacgtc ccgctgctgt
     2281 acgggatgcc gcccggcggc ttcgccgtgg acgggtcgcg gacgttcgcc gaggtgctcg
     2341 cggtgtggga gcgcgaggtc gcggtgagcc gggagatctg cgcggggcgc gacctggacg
     2401 agacggggcg gctcggtccc gagcagaggg tcgtcaccgg cggcgaggcc gtggtcagcc
     2461 tgcgctggat cctggtgcac ctcatcgagg agtacgcgcg ccacaacggc cacgcggatc
     2521 tgatcaggga gcgcatcgac ggcgtgacgg ggacctgaca tgtgcgaggg cgtccacgaa
     2581 ggcacgggcg gagacagggg cgcgggcgcg gccgggaacg ggagcgcgat cacgaggtcc
     2641 gcgcccgcgg cgcccgcacc cgcggcgccc gcgacgcccg agaagcgggg ccggaatcgg
     2701 aatcgggtgt tctattacag caccctccgc gccccctcgg ccaattcagc cgcgcattct
     2761 catgaattct ttatcagcac agcttcccgt attcttctgc ccgcttcccg gggacctaaa
     2821 cactaagatt tcgacagcgc caatcaccgc aatccggacg cgcccgccag agcccgcacc
     2881 cctgtgacgt actccacatc gcgtgcgttt tgtccgctat ggcccggtca aatcaagaca
     2941 aaagcccatg tcttcatgcg ggcgcgcacc cccgcgtgat aacacatggg gcgcgcttgt
     3001 gtaacccctt tccccgatgc aatccgagaa attgctcggt aaattcaatc cgcatgaccg
     3061 ctgcacaagc cgatctaccg accgacctgc aagcgaaatt cctggaaatg cccgaggggt
     3121 tgcgcatcga cagccccgcc gtcgccgacg gcgccgcgat ctggcggatc gcccgcgact
     3181 ccaaggtgct cgatctcaac tcctcgtaca gctacctgct gtggtgccgc gacttcgcgg
     3241 cgacctccgt ggtggcccgg ggcgccgacg gggagccggt cggtttcatc accggctact
     3301 tccggcccga gcgcccggac accctgctgg tctggcaggt cgccgtcgat cacgcacagc
     3361 gcggtcgcgg tctggccggc gccctgctcg acgggctgac cgcgaaggtc accgccggac
     3421 gtgcgccgat caccgtcgag acgaccatca cgccggggaa cacggcttcc gagcggctgt
     3481 tcctctccta cgcgcggcgc cacggcgcgg cggtcgagca gaagatcctc ttcgacgcgg
     3541 gtcttttccc cgatgacggg cacgagccag aagtgctcta ccgcatcggg cccgtcggcc
     3601 gctgaccggt ttcgctcccc ccgcatgtcc gcaacccccc actcccacgt ctttccatct
     3661 cccaggagat ccgctgtgac catcacccag cccgacctga gcgtcttcga gaccctggag
     3721 tcggaggtgc gcagctactg ccgcggttgg cctacggtct tcgaccgcgc gcagggcagc
     3781 cgcatgttcg acgaggacgg ccacgagtac ctcgacttct tcgccggagc cggctcgctc
     3841 aactacggcc acaacaaccc ggtcctgaaa cgcgcgctca tcgactacct ggagcgcgac
     3901 ggcatcgcgc acgggctcga catgtcgacg accgccaagc gcgccttcct ggagtcgttc
     3961 cagaacatca tcctgcgccc gcgcgacctt ccctacaagg tcatgttccc gggcccgacg
     4021 ggcaccaacg ccgtggagtc ggcgctgaag ctggcccgca aggtcaaggg ccgcgagtcg
     4081 atcgtgtcgt tcacgaacgc cttccacggc atgtcgctcg gttcgctcgc ggtgaccggc
     4141 aacgccttca agcgggcggg cgccggcatc ccgctggtgc acggcacgcc gatgccgttc
     4201 gacaactacc tcgacgggcg gaccccggac ttcatctggt tcgagcggct cctggaggac
     4261 cagggctccg gcctcaacca gcccgccgcc gtgatcgtgg agacggtgca gggcgagggc
     4321 ggcatcaacg tcgcccgcgc cgagtggctg cgcaagctcg ccgatgtgtg cgagcgctgg
     4381 gacatgctcc tgatcgtcga cgacatccag atgggctgcg gccgcaccgg cgcgttcttc
     4441 tccttcgagg aggcgggcat cacgccggac atcgtgacgg tgtcgaagtc catcagcggc
     4501 tacggcctgc ccatgtcgct ctgcctgttc aagccggagc tcgacgtgtg ggagccgggc
     4561 gagcacaacg gcacgttccg cggcaacaac ccggcgttcg tgacggccgc cgccgccctt
     4621 gaggcctact ggaccgacgg ctccgcgatg gagaagcaga cccgcgcccg cggcgagcag
     4681 gtcgagcagg tgctcgcctc gatcgtcgag gagaaccgcg ccgacatcaa ggagttccgc
     4741 ggccgcggcc tggtctgggg catcgagttc ctggacaagg accgcgcgag cgccgtggcg
     4801 aagcgggcct tcgaactggg cctgctcatc gagacctccg gccccgaggg cgaggtcgtc
     4861 aagctgcttc cggcgctgac gatcaccccc gaagagctgg acgaggggct gcgcacgctg
     4921 gcccgcgcgg tacgcgagac cgcctgaccc ggcggctgac cgagcccctc accccgtaag
     4981 agaaaaggaa ccgatacacc gtgatcgtcc gttcgttcaa ggacattgaa ggcaccgacc
     5041 ggcacgtgaa ggccgcgtcc ggcacctggg agagcaagcg catcgtcctc gccaaggagc
     5101 gggtgggctt ctcggtgcac gagaccatcc tgtacgcggg gaccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca ccaagggcga ggccgagctg accgacgaca
     5221 cgaccggtga gaagtacacg atcacgccgg ggaccatgta tctgctcgac gggcacgaga
     5281 agcacacgat gcggatcaag gaggacttcc actgcatctg cgtcttcaac ccgcccgtca
     5341 cgggtcgcga ggaccacgac gagaacggcg tgtacccgct gctcacggag ccggacgagg
     5401 cctgagagcc gcgcgcaggt cggctgaggt cggctgacat cccgcacagg gccgtacgag
     5461 aggagaggca ggcaacacca tgaccaccgc acccgaacgc accacagacc tgtacccgac
     5521 ccgaggcgcc gaagaggtgc tcatcgaacg caaggacccc gtcgtgtggt ccgagccggg
     5581 tgccgaaggg ccgatctcgg ccggggatct ggcggggttc gagcgggacg gtttcctggc
     5641 catcgaccag ctgatcaccc cggacgaagt gagcgtctac cacgccgagc tggagcggct
     5701 cgtcaacgac ccggcgatgc gcgccgacga gcgttccatc gtcgagccgc ggtcgcagga
     5761 gatccgctcc ctgttcgagg tgcacaagat cagtgagctg ttcggcaagc tggccgccga
     5821 cccgcgcgtg gtgggccggg cccggcagat cctcgggtcc gacgtgtacg tgcaccagtc
     5881 acggatcaac gtgaagccgg gcttcggggc ctccgggttc tactggcact ccgacttcga
     5941 gacctggcac gcggaggacg gcctgccgaa catgcggacc gtgtcggtgt cgatcgccct
     6001 gacgaagaac cacgacacca acggcggtct catgatcatg ccggggtcgc acaagacgtt
     6061 cctcggctgc gcgggcgcca ctccgaagga caactacaag cggtcgctgc agatgcagga
     6121 cgcgggcatc ccgtcgaacg agacgctgac ccacctcgcg tccgaccacg gcatccgcct
     6181 cttcacgggc gaggccggct cggccacgtg gttcgactgc aacgccatgc acggctcggg
     6241 ggacaacatc accccctatc cgcggtccaa cgtcttcatc gtcttcaaca gcgtggagaa
     6301 caccgcggtg gagccgttcg cggcgccggt ccgcaggccc gagttcatcg gcgcgcggga
     6361 cttcaccccg gtgaggtgac cggggccggg tccgcccggc ctgacgtacc cgggactcgg
     6421 cccggcgccc ctcgtgggcg ccgggccgat ttccgttcct cagcccgcca gcacgtccag
     6481 gagccggtcc acgtccgcct cggtgttgta gaggtggaac gtggcccgca ggtgacccgc
     6541 ccgcgccgcg acgacgacgc ccgcgcgggc cagctccggc tcgcggtcgg cgagccccgg
     6601 caccgagacg atgggcgacg cacccggcac gggcgcgtgg ccgaggccgg cgaggcccgc
     6661 gcggtagcgg gcggcgaggg ccaggtcgtg gcggtggatg gcgtcgacgc cgatctcctc
     6721 gatgagggag agcgccgccg cggccccgtg gtgggcgagg aaggcgggcg gctcgtcgaa
     6781 ccggcgggcg tcggcgacga cctcgccgat cgggccgtag gtcgagttcc aggcgtcggc
     6841 cgcggccagc cagccgccgt gcagcggcgc cagggactcc tgcgccgcct ccccgaccgt
     6901 caggaaggac gtgccgcgcg ggcagagcag ccacttgaag gcgccggtca cggagtagtc
     6961 gtacgcgccc gcgtcgagcg gctgccagcc cgccgactgc gtggtgtcgg ccagggtgcg
     7021 cgcgccgtgg gcggcggcgg cctcgcggat cgccgcgcag tccgccgtac gcccgtcggc
     7081 ggactgcacg gtggagaacg ccacgagcgc ggtcttcggc cgtatcgcgt cggcgagttc
     7141 gtccagcggc gcgaaccgcg gcttcaggtc gccgcgcagc acgaaggggt tgatcaggga
     7201 ggagaagtcg ccttcgggga agagcacttc ggcgcccgcg gggagggacg ccgcgatgag
     7261 gccgacgtgc acggcgaccg agccgcccgt cgccacgcgc gaggggtcca cgccgacgag
     7321 gcgcgcgaag gaggcgcggg cccggtcgac cgtggcgaag tcgcccgatc cgctccgccc
     7381 gccggagacg agttccgccg tcagggcctg tacggcctcg gcggcgcggc gcggcagcag
     7441 gccgcggacc gcggtgttga ggaaggtcgt ctccggcgcg aactcggcgc tgacgtggtg
     7501 gtcgatcccc agggaagtca tgggatcaac ctagggtgcc cggtcaggcc ccgggaaccg
     7561 cgcatccgtc gggaccgcac gcctcgccgt caccggcgtc cgcgagggtg gtcagcgggg
     7621 aacggccgcc ccacgcctgc tcgagggcct gggtgaacac ctcggcgggc tgggcgccgg
     7681 agacgccgta ccggcggtcg agcacgaaga acggcacgcc gttcgctccc agctcggcgg
     7741 cctcgcgctc gtcggcgcgg acgtcgtcgg cgtacctgcc cgggtcggcg aggacggcgc
     7801 gcacctcggc ctcgtcgagg cccgcctctg cggcgagctc gacgagccgc tcgtcgtcgg
     7861 cgaagacgga ccgctcctcg gcgaagttcg cccggtagag cagaccgatc agctcgtcct
     7921 ggcgcccccg ctccttggcg aggtgcagca cacggtgcat gtcgaaggtg ctgccgtggt
     7981 cgcggccttc gatgcggtag tcgaggccct cggcggcggc gttctccccg aggcgccgct
     8041 cgccctcgcg ggcctgctcc tcggtcatgc cgtacttgtt catgagcatc ggaaggacct
     8101 gcccggtgtc gcccttggcg cggccggggt ccagctcgaa ggaccggtgc acgacctcca
     8161 cggcgtcgcg gtgcgggaag gcggcgagcg ccttctcgaa gcgggccttg cccacgtagc
     8221 accaggggca ggcgatgtcg ctccagatct cgacgcgcat gtgtggctct ttccgggtcg
     8281 tgcggtacgc gggtgcgtga cggtcggcgc ggagggtccc ctgcgggatc cctccgccac
     8341 cgccgtcaac acacgggcga agcggcgcat tcccctacga gccgtcgcgc aggcccttcg
     8401 gccagttcgg gcggaactcg atgtggtcgt acgtcaccac gcacccctcc cccaccggcg
     8461 actgcgccat gaagccgacg agggcggcgc ccgccgactc ctcgtcgccg agcgagaaga
     8521 tccgcacgaa cgtccaccgc tcgccgtccg ccgacgcgtg gaaggcgaac gccgagcccg
     8581 tgcggctgat gcgcagccag gcgctggcgc cgtccacggt gaaggcgttg gcgtcgtcgg
     8641 agtggccgcg cgtcacgacg gtgcagaccg tcggctcgtc cggggagcgc tccaggcaga
     8701 gcttggccca ctcccgctcg ccgacgtgca gatagagcac gcccgcgtcg aagggcgccg
     8761 cgaagccgac cgtgacgcgg gcgatcagct ggaagtcgcc ttccggcgcg cccagcaggc
     8821 gcggcgcgtc ggcggcgggg tcgagcgcct cgccggtcgg cggcacgaag cggtcctgcc
     8881 gggcgcccgc ccagccggtg agcaccccgt cctcgtggga ccagtggccg tcggggccgt
     8941 agccgcgcag ggagaagggg agctggggga ggtcgaggtc catggcgtca gtgttccagg
     9001 gtcccgtcga cccgccgcgg cagccccagc gggttgtcgt cgcgcagctc gggcgggagc
     9061 aggggctccg gggtgttctg gtacgcgacc gggcgcagcc agcgctcgat ggcggtgccg
     9121 cccaccgagg tcgacgtgga cgtggtggcc gggtaggggc ctccgtggtg ctgggcgggg
     9181 gcgacggcga cgccggtggg ccagccgtcg acgaggacgc ggcccgcgag cggcgtgacc
     9241 tcggccagga gttcggtgcc gcgggggctc acgcccgccg cctcgtcgga ggagagctgc
     9301 acggtggcgg tgaggttgcc gggcaggcgg gagaggacgg cggtgatctc cgcggcgtcc
     9361 ttgtagcgcg cgacgacggt gacggggccg aagcactcct cgatgagcag gtcgtgctcg
     9421 ccctcggcgg cgagccgtgc cgcggggacg gtgaagaagc ccgcgctcac ggtgtgctcg
     9481 ccgcccgcgc cgggcgtgac gggggagtcg acgtccgcga ggcccgcgcg ctcgcggacc
     9541 ccggcgacga agttgtcccg catccggtgg tccaggagca ctccggcgtc ggtgtcgccg
     9601 accgcatcgg tcagggactt cacgagccgg tcgccgcccg cgccctcggg ggcgaggacg
     9661 aggccgggct tcacgcagaa ctggccgacg ccgagcgtca tcgagcccgc gaggccggtg
     9721 ccgatctcct cgccgcgctc ggcggcggcg gcctcggtga cgacgacggg gttgagggag
     9781 cccagctcgc cgtggaaggg gatcggcact ggccgggccg ccgccgcgtc gaagagggcc
     9841 cggccgccgc ggatggagcc ggtgaacccg gccccggcca ccagcgggtg cttgaccagc
     9901 tcgatgcccg cctcgaagcc gtgcaccagg ccgacgacgc cctcggggat gtcgtgccgg
     9961 gcggcggccc tgcgcagcac ggaggcgacc agctcggagg tgccggggtg gtcggggtgg
    10021 gccttgacga cgaccgggca gcccgcggcg agcgcgctcg cggtgtcgcc gccgggcacg
    10081 gagaaggcga aggggaagtt ggacgcggag tagacggcga cgacgccgag ggggatcttg
    10141 tagcggcgca ggtcggggat gggcggggtc gccgacccgt cggggtggtc gatgacgact
    10201 ccgaggaagg cgccctcgtc gacgatgtcc gcgaaggcgc gcaactggaa gcaggtgcgg
    10261 gcgagttccc cggtgagccg gaccgggccg agcgcggtct ccgcgtccgc ggcctccacg
    10321 agccggtccc cggcctcctg gaggaggtcg gcggcggtgc gcaggaaggc ggtgcgcacc
    10381 gcgcggtcgg ccagggcggg ggctg
//
