LOCUS       NZ_CP060742            22512 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces alfalfae strain XN-04 chromosome, complete genome.
ACCESSION   NZ_CP060742
VERSION     NZ_CP060742.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces alfalfae
  ORGANISM  Streptomyces alfalfae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     The Biocontrol and Plant Growth-promoting Properties of Streptomyces
            alfalfa XN-04 Revealed by Functional and Genomic Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254035)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2020) College of Plant Protection, Northwest A&F
            University, No. 3 Taicheng Road, Yangling 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141485.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 05:42:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,142
            Genes (coding)                    :: 6,967
            CDSs (with protein)               :: 6,967
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 75 of 175
            Pseudo Genes (incomplete)         :: 141 of 175
            Pseudo Genes (internal stop)      :: 22 of 175
            Pseudo Genes (multiple problems)  :: 59 of 175
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:18:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 255747
            Orig. end                         :: 278259
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22512
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="3"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22512
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22512
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12512](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12512)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            338..3595
                     /locus_tag="H9W91_RS01020"
                     /old_locus_tag="H9W91_01015"
     CDS             338..3595
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481642.1"
                     /locus_tag="H9W91_RS01020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01015"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_245001459.1"
                     /transl_table=11
                     /translation="MRALRQELDFGLLGPLVVRRAGEVLDPGRRKQRLLLVRLLLADGC
                     AVTPQTLCEDLWAEEAGRAPGGAMASLHAHVSKVRAVLEPQHLRVRGTFAVLVTEPAGY
                     ALRVPPESRDTVRFERTAARAHQLLGQGQPEHVLRETDRALGMWRGAALADAAHHLFAA
                     HESARLEELRQTVRETRTTALLLSGRLPEAVDAAQELTREHPLRETGWALLLRALYLAG
                     RHPEALGHYADLRRRLADGLGLEPGPALRALHEGILRHELPPLPLPGTAGQPARTADRG
                     CPGHPAAPAASDGKPAASPGGQDVTGPAGARPAAATGPDADPSDISGDAVARGPAGEAS
                     PAGTVAEASAGPAGPADAHGHRPGDSGAEDIRPVRPAQLPRDLPVFAGRAAESAWLSAV
                     NGAPGRPAPSGAPVVVIGGAPGVGKTTFAVHHAHRIASSFPDGQLFVNLCGFHPTAPAV
                     EPGDALHGFLTALGVPAQRIPEDTDGRSTLFRSLLAGRRVLLVLDNARDEQQVRPLLPA
                     GGGCLTLVTSRNRLPGLIATEGAKTLTLALPSQAEAHETLTRRLGAERLDAEPAATADI
                     IQLCGRLPLALAVVAARAELDPTFPLHAVADDLRHAHGGLEAFTGFDDTTDVRSVFSWS
                     YRSLDEAVARLFRLLALHPGPDITAAAAAGLAGLPLPRAQRLLSVLTRSCLVEQPLPGR
                     YALHDLLRGYATELTHTHDDTSERHHATLRVLDHYMFTAYQGNRLFKQDSLPELDLGSP
                     SADVTPEDLTTVGQANRWFAAEHRVLAALLHTAVGQRMDHHITGLAWSLKEYLNRQEYW
                     PEAITALTPALDVARRRGDRLEEGRCLRHIGGIHGYLGHEEQALCHLNRAITILGELGA
                     VGEQARAHYALACALHLFGRTPEAVVASERGLALSRATGDQLWTAECLVELAWFHSNLG
                     QHEKGLTLSQEAMSLFQLLDAPWQQAQNWDVLGHILRQLGRYEESVTTYRQAVEAFEEL
                     GDQRNAVGALMRLGDTRLAMGDSEAARAEWTRALAGAEQHALSRSAQEVRERLAALDSE
                     MPLDPAAPAESAGDARAPAPASGAPAHKVPVPVSRQV"
     gene            complement(3686..4519)
                     /locus_tag="H9W91_RS01025"
                     /old_locus_tag="H9W91_01020"
     CDS             complement(3686..4519)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (25-264). E-value:
                     5.9e-42. Score: 135.9. Matches aSDomain:
                     nrpspksdomains_H9W91_RS01025_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116052.1"
                     /locus_tag="H9W91_RS01025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01020"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_211824741.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-29, bitscore:
                     102.2, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9e-22, bitscore:
                     77.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFITVGQENSTSIDLYYEDHGTGQPVVLIHGYPLDGQSWEKQAA
                     ALLKAGYRVITYDRRGFGRSSRPTTGYDYDTFAADLNTVLETLDLHDAVLVGFSMGTGE
                     VGRYLGTYGSARIARAAFLASLEPFLLKTDDNPEGVDGSVFEGIEQAVTADRYAYFTAF
                     CEDFYNLDENLGTRISEEAVRNSWNTAAGASWYASLACVATWTTDFRADVAAIDVPALI
                     LHGTADRILPIEATGEPFHRALPQAEYVVIEGAPHGLLWTHAQEVTDALLAFLAK"
     aSDomain        complement(3728..4444)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS01025_Abhydrolase_1.1"
                     /evalue="5.90E-42"
                     /label="H9W91_RS01025_Abhydrolase_1.1"
                     /locus_tag="H9W91_RS01025"
                     /protein_end="264"
                     /protein_start="25"
                     /score="135.9"
                     /tool="antismash"
                     /translation="PVVLIHGYPLDGQSWEKQAAALLKAGYRVITYDRRGFGRSSRPTT
                     GYDYDTFAADLNTVLETLDLHDAVLVGFSMGTGEVGRYLGTYGSARIARAAFLASLEPF
                     LLKTDDNPEGVDGSVFEGIEQAVTADRYAYFTAFCEDFYNLDENLGTRISEEAVRNSWN
                     TAAGASWYASLACVATWTTDFRADVAAIDVPALILHGTADRILPIEATGEPFHRALPQA
                     EYVVIEGAPHGLLWTHA"
     gene            complement(4602..5432)
                     /locus_tag="H9W91_RS01030"
                     /old_locus_tag="H9W91_01025"
     CDS             complement(4602..5432)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Thioesterase (45-254). E-value: 1.9e-08.
                     Score: 27.0. Matches aSDomain:
                     nrpspksdomains_H9W91_RS01030_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481660.1"
                     /locus_tag="H9W91_RS01030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01025"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_076688197.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.3e-18, bitscore:
                     66.3, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 5.2e-06, bitscore: 25.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSTRPSRRTTLAGLTALAALSTVTVALPGAAGASPRHKALPTIV
                     LEHGAFADAASWDGVVKRLQRSGYPVVAAANPLRGPANDAAYLRSVVDHIDGPVVLVGH
                     SYGGTVISQAAAGLEDKVKALVYIAAFLPDAGESSLGLTDKFPGSTLGQVIEPVNYTLP
                     DGGQGADVYIKPEKFRKQFAADVPADKARLMAAGQRPVAAAALEEKSTTAAWKAIPSWS
                     LVTTKDRNIPVAAQRFMSARAHAHTTEIDASHAVTVSRPDAVTRIVEKAARTAR"
     aSDomain        complement(4671..5297)
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9W91_RS01030_Thioesterase.1"
                     /evalue="1.90E-08"
                     /label="H9W91_RS01030_Thioesterase.1"
                     /locus_tag="H9W91_RS01030"
                     /protein_end="254"
                     /protein_start="45"
                     /score="27.0"
                     /tool="antismash"
                     /translation="LEHGAFADAASWDGVVKRLQRSGYPVVAAANPLRGPANDAAYLRS
                     VVDHIDGPVVLVGHSYGGTVISQAAAGLEDKVKALVYIAAFLPDAGESSLGLTDKFPGS
                     TLGQVIEPVNYTLPDGGQGADVYIKPEKFRKQFAADVPADKARLMAAGQRPVAAAALEE
                     KSTTAAWKAIPSWSLVTTKDRNIPVAAQRFMSARAHAHTTEIDASH"
     CDS_motif       complement(5103..5147)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H9W91_RS01030_0001"
                     /evalue="1.20E-05"
                     /label="NRPS-te1"
                     /locus_tag="H9W91_RS01030"
                     /protein_end="110"
                     /protein_start="95"
                     /score="17.9"
                     /tool="antismash"
                     /translation="DGPVVLVGHSYGGTV"
     gene            5869..6438
                     /locus_tag="H9W91_RS01035"
                     /old_locus_tag="H9W91_01030"
     CDS             5869..6438
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114144.1"
                     /locus_tag="H9W91_RS01035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01030"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_237283502.1"
                     /transl_table=11
                     /translation="MDARVTTSAPARMRARHISSDVDCDAFIEEIYDQYGPLLVRYATR
                     LLDGDWHRAEDMIQEAATRAWKHARFLGARREHVRPWLYTVVKNLVIDHHRARRCRPLE
                     QVPAQELDAVWDGLETTLTSHVILEALKELNEQQRAVIHLMYYMECSVAQAAEYLGIPP
                     GTVKSRAFYAIRALRTTLAERGVVGQ"
     gene            complement(6850..7569)
                     /locus_tag="H9W91_RS01040"
                     /old_locus_tag="H9W91_01035"
     CDS             complement(6850..7569)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176075.1"
                     /locus_tag="H9W91_RS01040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01035"
                     /product="response regulator transcription factor"
                     /protein_id="WP_245001460.1"
                     /transl_table=11
                     /translation="MSAQNDADRQDHAAPAPAPAPAPARRVLLVDDHPLFREGLAAAID
                     LDDHFTVVAEAASGEAALTETARTDPDLVVMDLCLPGISGIEATRRLLTAHPHLRIMVM
                     TMSEDDDSLLAAMRAGAHGYLLKGALREEVLRALHTVARGGAVFGPQMTERLSALFAAF
                     GRTPAKEAFPTLTARERDILDLLAAGLTYRQIAQRLCVVEKTVRNHVGAIFSKLQVHDR
                     AAAIVRARDAGLGPQPQ"
     gene            complement(7475..9580)
                     /locus_tag="H9W91_RS01045"
                     /old_locus_tag="H9W91_01040"
     CDS             complement(7475..9580)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330919.1"
                     /locus_tag="H9W91_RS01045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01040"
                     /product="histidine kinase"
                     /protein_id="WP_211824742.1"
                     /transl_table=11
                     /translation="MALGLLAAVLVVKLTVLSVSGEQWRAVHYSPPQTIAEVMRLAVGL
                     PVWVIGVLLLADRSGRGMGALLLAAGVVLVVPRTVSDLLAFIGDWSAPVDVTVLALAAV
                     ALAGHPLSVLVFPVCFLREPAVRRCRWYVIAPAAAAHAGHGALWFLGVPGAPPFFSPWS
                     GTATAQWALQHIGPVSHLVDWVSGAAALAVTAAVAHCSLSGDDAAERRRWLLLAVAYAV
                     CVSLLLGQWWPERWTMAARAGGGTVWVVVICLTVARSDVWRLQRATVHRLSVAFVLTVF
                     GTAAVGAALVAWAALPAVRSTASLTVAGCALLAGWVTRPVVVQSSLRVERFFYGSRARP
                     HHAMRKLVTRLQEAPDPRDVPEQICRSAVEDLGVSGAAVEVDTRSGVRRLGAVGVPVIE
                     PKQVFALRHHGQVVGRLEVARDGTSTPVERDSDLLSLLADQAGPALAALRLVEETQAAR
                     ESLVLAREEERRRLRREIHDGLGPQLAAVQMRLGTAQACVGPQSSAPRHLRAAAEGLSE
                     ALTEVRRITAGLTPAVLVERGLLEAIRILAHRLSTEVVQVVVADPATKMPVLAPAVETA
                     AYRIAAEAMTNAVRHSGARRVLVGLAVTPASLTVKVSDDGNGLPGGGVPGTGLASFTER
                     AEEIGGTTGVHTGPHGTAVHATLPLTRQGRELEEGHERPERRGPAGSCGTGTGTGTGTG
                     SASTAGG"
     gene            complement(9643..9864)
                     /locus_tag="H9W91_RS01050"
                     /old_locus_tag="H9W91_01045"
     CDS             complement(9643..9864)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H9W91_RS01050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H9W91_01045"
                     /product="hypothetical protein"
                     /protein_id="WP_123985647.1"
                     /transl_table=11
                     /translation="MRSFAAVLGVIIAMTAVSTSPAQAAAEERLIAHYHSKSGCLDAGA
                     RGKRQGKWATYRCLPSWDGPGPWGLWVS"
     misc_feature    9875..9889
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            complement(10001..10420)
                     /locus_tag="H9W91_RS01055"
                     /old_locus_tag="H9W91_01050"
     CDS             complement(10001..10420)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /locus_tag="H9W91_RS01055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01050"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_211824744.1"
                     /sec_met_domain="PF13471 (E-value: 9.4e-23, bitscore: 79.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGLVTTEVSERPPFGLLLTARCAVVVAFGLARLSPGGLRRVLRL
                     ISVRARPAGHEQALRARKAVVAASVRCAGPWCLPRSIATALLCRAGGTWPDWHTGVCAE
                     PFRAHAWVVADGEPVGESNASLVPFHSVIVVRAGR"
     gene            complement(10417..10674)
                     /locus_tag="H9W91_RS01060"
                     /old_locus_tag="H9W91_01055"
     CDS             complement(10417..10674)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330917.1"
                     /locus_tag="H9W91_RS01060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01055"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_062769902.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 5.6e-19,
                     bitscore: 66.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7.6e-10, bitscore: 37.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRFALPPHVTNTETEHGAVLLNEKTGRYWQANDAGARVLRCLLDG
                     GTAETAAAALRPGDPKAYERALRDVRAMIDGLRDAGLVIS"
     aSDomain        complement(10423..10665)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_H9W91_RS01060_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.20E-21"
                     /identifier="RREFam002.1"
                     /label="H9W91_RS01060"
                     /locus_tag="H9W91_RS01060"
                     /protein_end="84"
                     /protein_start="3"
                     /score="66.7"
                     /tool="antismash"
                     /translation="ALPPHVTNTETEHGAVLLNEKTGRYWQANDAGARVLRCLLDGGTA
                     ETAAAALRPGDPKAYERALRDVRAMIDGLRDAGLVI"
     gene            complement(10674..12512)
                     /locus_tag="H9W91_RS01065"
                     /old_locus_tag="H9W91_01060"
     CDS             complement(10674..12512)
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /locus_tag="H9W91_RS01065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01060"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_211824752.1"
                     /sec_met_domain="Asn_synthase (E-value: 8.3e-33, bitscore:
                     113.2, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFIVLPDHPAADAVLALSPRRAGDKVVRHPSGRPWVIGSWCDEE
                     LTAASAGSNAVVLLGRTRIGETELARKLAVMRSVTDMDSLSRSTHGCFHLLASIGGQVR
                     AQGSLTTVCQVFYGSVAGVAVAADRPQSVAALTGATIDEELVALRLLSPTAPHPLSQAC
                     LWRGVTALPPGHYLLMPANSDARPVRWWTPPEPSVPLAQGADTLRRALEAAVETRTAHS
                     ATVSTDLSGGMDSTSLCFLAAPRAARLVTYHHVPLDPSNDDGIWAGRCAADLPDARHVK
                     EHSGAAPAWYAGCLSTDEDVEGPFPLIRTRAKLEHLARTMAALGSTAHLQGIGGDELFH
                     ARAACAHALARAHPLRSARQVRVVRSMSRWTTSATIGYLLSGGTYARWLRSSAALLGPE
                     PRKRATGPAWESPPAMPVWATPRAVDAVRSLIRAAADGEPEPLAPLPVQHDMLSVAQLC
                     GEMTRRASRFTARHGVSYEAPYLDDRVVEAAMSVRLEDRIAAGRYKPVLATAMRDVLPA
                     YAQGRATKGDYSGELYSGLRQHRAELLELCEESVLVGMGLVDRVALRGLLLGLHPDTSP
                     FMAFDSTLACELWLRSLYRDNRSGTTASLPPNREEI"
     gene            complement(12613..12735)
                     /locus_tag="H9W91_RS01070"
                     /old_locus_tag="H9W91_01065"
     CDS             complement(12613..12735)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056410.1"
                     /locus_tag="H9W91_RS01070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01065"
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_103557564.1"
                     /transl_table=11
                     /translation="MQSEKLVYDMPLIVEVGGFRELTRSVRPGHWIDFFGGWWL"
     misc_feature    14221..14230
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(14480..15634)
                     /locus_tag="H9W91_RS01075"
                     /old_locus_tag="H9W91_01070"
     CDS             complement(14480..15634)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087552.1"
                     /locus_tag="H9W91_RS01075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01070"
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /protein_id="WP_211824754.1"
                     /transl_table=11
                     /translation="MQQLKLGIISQTRKENERRLPIHPAHFERIDADLQPSIYLQAGYG
                     EHFGVSDRQLAPWVAGFRSREELIAECDVILLTKPLHEDLAELREGQVLWGWPHCVQDE
                     KVTQAAIDRRLTLIAFEAMNHWTREGSFNLHVFHKNNELAGYSSVLHAMQLTGATGDYG
                     RRRRAAVIGFGATARGAVTALSALGVHDVDVLTARGVAAVSSPIHSARIVHFDHDVADE
                     TLDPRRSVVLTEHGPEPLADFLAGHDIIINCVLQDTAAPLMFLLEEDLPKLAPGTLVID
                     VSCDQGMGFAWARPTTFTEPMFTVGGHVHYYGVDHSPSYLWDAVTWENSEALIPFLRPV
                     LEGPAGWDGNRTIHRAIEIRGGTVQNPAVLAFQHRAEQYPHALL"
     gene            15983..16854
                     /locus_tag="H9W91_RS01085"
                     /old_locus_tag="H9W91_01080"
     CDS             join(15983..16343,16343..16854)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /locus_tag="H9W91_RS01085"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="H9W91_01080"
                     /product="IS5 family transposase"
                     /protein_id="WP_245001477.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MDQSVVGPGVPLTDAQWARIEPLLPDRTPRRGGRWRDHREVIDAL
                     AFKFQTGTQWVHLPEKYGNWRGVYNRLRMWAVDGTWERVFTALVAQADADEDVSWAVSV
                     DSTIVRAHQHAAGARKKGAPAGEPEDHAIGRSRGGLTTKIHLAADGRCRPLAFVLTAGQ
                     AGDAPAFTDVMARLRVPRRRGRPRTRPDLVLADKAYSSRAIRDHLRRRGIRAVIPERAD
                     QQANRRQRGQTGGRPPAFDREAYKQRNTVERCINRLKQWRGIATRYEKTATVYLAGLHI
                     AGIFLWSAR"
     gene            complement(16870..17019)
                     /locus_tag="H9W91_RS01090"
                     /old_locus_tag="H9W91_01085"
     CDS             complement(16870..17019)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H9W91_RS01090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H9W91_01085"
                     /product="hypothetical protein"
                     /protein_id="WP_211824756.1"
                     /transl_table=11
                     /translation="MWWWTGSTAGDSPQKALKAGALGRRVTVTGVTGVGFVFRPQPTIC
                     LLWH"
     gene            complement(17343..17942)
                     /locus_tag="H9W91_RS01095"
                     /old_locus_tag="H9W91_01090"
     CDS             complement(17343..17942)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054496.1"
                     /locus_tag="H9W91_RS01095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01090"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_237283500.1"
                     /transl_table=11
                     /translation="MPSGVHIHNVRGQLFEAAERVLLRGGPDALTSRAVTEEAGCAKGV
                     LHRHFTDFDAFLVELVLDRVHGIEDHATTLRAAAGTGGVVDNLADALQELFGPVTVAIV
                     ALITFRDSLRARLREAGLTGIPLVMQGTAMIAAYLTAERGMGRLAGDADIDTLAPTLVG
                     AGHLLFADRTNAPPQPDAIRKMVTTVIAGALRPLQP"
     gene            18004..18834
                     /locus_tag="H9W91_RS01100"
                     /old_locus_tag="H9W91_01095"
     CDS             18004..18834
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745714.1"
                     /locus_tag="H9W91_RS01100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01095"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_205576637.1"
                     /transl_table=11
                     /translation="MPTLSQGQPDASGPEPHRHRQTAESFGVDAERYDRARPPYPQALV
                     DRIVTASPGLHVLDVGAGTGIEARQFQAAGCTVLGVEPDERMAAFARRSHIEVEVARFE
                     DWQPAGRQFDAVIAGTAWHWVDPVAGAAKAAQVLGPGGRLAPFHHVPQLPVELIDTLAE
                     ALQRVAPYSPFNPGLLKGSAVDAYQPLFTKIADGIRQTGSFTEPEQWRFTWERTYSREQ
                     WLDQLPTFGGLTQLPADKLAKVLDSAGTAIDKLGGHATMPYTSIVITSTRSHTV"
     misc_feature    18013..18015
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(19486..19935)
                     /locus_tag="H9W91_RS01105"
                     /old_locus_tag="H9W91_01100"
     CDS             complement(19486..19935)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092127.1"
                     /locus_tag="H9W91_RS01105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01100"
                     /product="DNA-binding protein"
                     /protein_id="WP_076688185.1"
                     /transl_table=11
                     /translation="MSSTPEQPTDPFTPPQPEQARVHRAHASLFRIAERHAATDEQRHR
                     QVHPSVIGPHEAVRLVSFLLSGAAQLDDAEPEVDHADITAALSLVPMVRGEMDELETGL
                     LQMARGRGMTWQEIAFGLGLGTPQAARQRYERLASRAASDAEGPE"
     gene            20445..21134
                     /locus_tag="H9W91_RS01110"
                     /old_locus_tag="H9W91_01105"
     CDS             20445..21134
                     /GO_function="GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982175.1"
                     /locus_tag="H9W91_RS01110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01105"
                     /product="haloacid dehalogenase type II"
                     /protein_id="WP_211824758.1"
                     /sec_met_domain="HAD_2 (E-value: 0.0012, bitscore: 17.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSELAIDAIVFDVLGTLVDEPAGIRAGIRELDPSLDDPGVEQLLS
                     LWQQHIDREQRRVLDGVRPYLTSDVLDLEAARLVADAAGVDDPAAVAALAMSGRRLPPW
                     PDTVAGLSRLAERFPLIGLSNASRTALLGLNAHAGLRWHQALSAEEARTYKPDPAVYQL
                     AVTVSGRPPERLLMVAAHAWDLRGAQSLGLRTAYVARPVGDPPTPSDGFDVHADDLAGL
                     AAQLDHP"
     misc_feature    20454..20456
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(<21173..>21356)
                     /locus_tag="H9W91_RS36010"
                     /pseudo=""
     CDS             complement(<21173..21356)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981454.1"
                     /locus_tag="H9W91_RS36010"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEEAYPFRRNRRHLRRRQIKHTIPEPGNQRASVNAVPAAAVGPPA
                     RQNDLPTQERS"
     misc_feature    complement(21464..21482)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.81"
                     /tool="antismash"
     misc_feature    complement(21475..21489)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            complement(21650..22165)
                     /locus_tag="H9W91_RS01115"
                     /old_locus_tag="H9W91_01110"
     CDS             complement(21650..22165)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180149.1"
                     /locus_tag="H9W91_RS01115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9W91_01110"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_198501443.1"
                     /transl_table=11
                     /translation="MSSPSAPAHTDVVTARLVLRSWTKSAATAVLGGARSPHWADDFPA
                     EGDRVIAGLFTQYPDWLGAYGHRLIIERDSGLVVGSIGLFWPPKDGVLEIGYGIVASRR
                     GRGYAPEATQALAAFALTAPDVHTVSADVELSNPASVRVLEKAGFHRWTTAGTIARFRI
                     ALPNHDRR"
ORIGIN
        1 cagcagccac accggccctg atccccggcg agtcgcggtg cgtgatcgat catgggcccg
       61 tacgcccgga gccgggcaca cggttacgct tgcacggcac ccgccgcacg aagtcaaggg
      121 gtatgggtca cgacggtcac gaccccagcg gtcggccgga gcggacccgt tacccgtacc
      181 tcgcgccccc ggggggacgc gagagggttc tccccgcttc ggacgacccg tgccgctttc
      241 ccctccccag gcccgggagg ggcgccgcga cagtgaccgc gtgccgtcgt gcgcggagag
      301 gcggcgagaa tgttgagcaa ggtactgggt gggatgggtg agggctttga ggcaggaact
      361 cgacttcggg ctgctgggcc cgctggtggt gaggcgggcg ggcgaggtcc ttgatccggg
      421 gcggcgcaag cagcggctgc tcctggtacg actgctgctc gcggacggct gtgcggtcac
      481 tccccagacc ctgtgcgagg acctgtgggc ggaggaggcg ggtcgcgcgc cgggcggggc
      541 gatggcctcc ctgcacgccc atgtcagcaa ggtccgcgcg gtcctggagc cgcagcacct
      601 gcgcgtgcgc gggacgttcg cggtcctggt caccgagccc gccggctacg ccctgcgcgt
      661 cccgcccgag agcagggaca ccgtgcgctt cgagcggacg gccgcccgag cgcaccagct
      721 gttgggccag ggacagcccg aacacgtgct gcgggagacc gaccgggctc tggggatgtg
      781 gcgtggtgcc gcactcgccg atgccgcaca tcacctgttc gccgcccacg agagcgcgcg
      841 gctggaggaa ctgcggcaga ccgtccggga gacccgcacc accgccctgc tcctgagcgg
      901 acggctgccc gaggccgtcg acgcggcaca ggagctgacg agagagcacc cgctgcggga
      961 gaccggctgg gccctgctgc tgcgtgcgct gtatctggcc ggcagacacc ccgaggcact
     1021 ggggcactac gcggatctgc gccggcgcct cgccgacggc ctcggcctcg aacccggccc
     1081 cgcgctgcgt gccctgcacg agggcatcct ccgccatgag ctgccgcccc tgcctctgcc
     1141 gggaacggcg ggacagccgg cccgcaccgc agaccgcggc tgtccggggc acccggctgc
     1201 gcctgcggca tccgacggca agcccgcggc ctctcccggg ggccaggacg tcacaggacc
     1261 tgccggtgca cgacctgctg ccgccacagg cccagatgcc gaccccagtg acatctccgg
     1321 tgatgccgtg gcccgcgggc cggccggcga ggcgtccccg gccggcacgg tcgccgaggc
     1381 atccgccggt ccggcaggtc cggccgatgc tcacggccac cggccgggag acagtggcgc
     1441 ggaggacatc cgaccggtgc gcccggcaca actgccacgg gacctgccgg tgttcgcggg
     1501 ccgggcggcc gagagcgcct ggctgtccgc cgtgaacggt gcgcccggcc ggccggcgcc
     1561 gtccggcgct cccgtcgtcg tcatcggcgg cgccccggga gtcggcaaga ccaccttcgc
     1621 cgtccaccac gcccaccgca tcgcctcctc cttccccgac ggacagctct tcgtcaacct
     1681 ctgcgggttc catccgaccg cacccgccgt ggagcccggc gacgccctgc acggcttcct
     1741 caccgcgctc ggcgtccccg cccagcgcat ccccgaggac accgacggcc gcagcacgct
     1801 gttccgcagc ctcctggccg gccggcgggt cctcctcgtc ctggacaacg cccgcgacga
     1861 acagcaggtc cgacccctgc tgcccgcagg cggcggctgc ctcaccctgg tcaccagccg
     1921 caaccggctc ccgggcctga tagcgaccga aggggccaag accctcaccc tcgcgctgcc
     1981 ctcacaggca gaggctcacg agacgctgac acggcggttg ggggccgaac gcctggacgc
     2041 cgagcccgcc gccacggccg acatcatcca gctgtgcgga cgtctccccc tggcactcgc
     2101 cgtagtggcc gctcgcgccg aactcgaccc gacgttcccc ctgcacgcag tcgccgatga
     2161 cctgcgccat gcccacggtg gcttggaggc gttcaccgga ttcgacgaca ccactgatgt
     2221 ccggagcgtg ttctcctggt cctaccggtc cctggacgag gccgtcgcac ggctcttccg
     2281 gctcctggcc ctccatcccg ggcccgacat caccgccgcg gctgccgccg gcctcgctgg
     2341 gctccctctc ccccgggccc aacgcctgct ctccgtactc acccgctcct gcctcgtcga
     2401 acaaccactg ccaggccggt acgccctgca cgacctgctg cgcggctacg ccaccgagct
     2461 cacccacacc cacgacgaca ccagcgagcg tcaccacgcc accctgcggg tccttgacca
     2521 ttacatgttc acggcctacc aaggcaaccg gctgttcaag caggactcac tgcccgaact
     2581 cgacctcggc tcaccgagcg cggacgtcac cccggaggat ctcaccaccg tggggcaggc
     2641 gaaccggtgg ttcgccgccg agcaccgggt actggccgcc ctgctgcaca ccgccgtcgg
     2701 ccagaggatg gaccaccaca tcaccggcct cgcctggtcg ctgaaggagt acctcaaccg
     2761 ccaggagtac tggccggaag ccatcaccgc gttgactccc gccctggacg tggcccggcg
     2821 ccggggcgac cgcctcgaag aaggccgctg cctgcgccac atcggtggca tccacggcta
     2881 cctcggtcac gaggaacagg ccctgtgcca cctgaaccgc gccatcacga ttctcggtga
     2941 actcggcgcc gtgggcgagc aggcccgcgc ccactacgcc ctggcgtgcg ccctgcacct
     3001 gttcggccgg accccggagg cggtggtggc ttccgagcgg gggctcgccc tgtcccgggc
     3061 gacaggggac cagctctgga ccgccgaatg cctcgtcgag cttgcctggt tccactccaa
     3121 cctggggcag cacgagaagg gcctcacgct gagccaggag gccatgtccc tgttccagct
     3181 tctggacgcc ccctggcagc aggcccagaa ctgggacgtt ctgggccaca tcctccgtca
     3241 gctcggccgg tacgaggaat cggtgaccac ctaccggcag gcggtggaag ccttcgagga
     3301 actgggtgac cagcggaatg ccgtcggcgc gctgatgcgc ctgggtgaca cacgtctggc
     3361 catgggcgac tcggaggctg cccgggctga atggacccgg gccctcgccg gcgccgaaca
     3421 acacgcactg tcccgcagcg cccaggaggt cagggagcgg ctggccgctc tggacagcga
     3481 gatgcccctc gatcccgccg cgccggcgga gtccgccgga gacgcccgcg ccccggctcc
     3541 ggcgagcggc gcccctgcgc acaaggttcc cgtgcctgtc tcgaggcagg tctagcggat
     3601 ctcccggagc cgatccgatg gcgcgggcgt tcgttgccag ggcggccgct ccgccggagc
     3661 cgcggagcgg ccgccctggt gtgggtcact tggccaggaa ggcgagcagg gcgtcggtga
     3721 cttcctgggc gtgggtccac agcagaccgt gcggggcgcc ctcgatgacc acgtactcgg
     3781 cctgcggaag ggcccggtgg aagggttctc cggtggcctc gatcgggagg atgcgatcgg
     3841 cggtgccgtg caggatcagc gcgggtacgt cgatcgcggc gacgtcggcg cggaagtcgg
     3901 tcgtccaggt cgccacgcag gccagcgagg cgtaccagga ggcgcccgcg gcggtgttcc
     3961 agctgttgcg tacggcctcc tcgctgatcc gggtgccgag gttctcgtcg aggttgtaga
     4021 agtcctcgca gaacgcggtg aagtaggcgt accggtcggc ggtgacggcc tgttcgatgc
     4081 cctcgaagac actgccgtcg accccctcgg ggttgtcgtc ggtcttgagc aggaacggtt
     4141 cgagggaggc gaggaaggcg gccctggcga tccgggcgga cccgtaagtg cccaagtaac
     4201 ggccgacttc gccggtaccc atggagaatc cgaccaggac ggcgtcgtgg aggtcgaggg
     4261 tctccaggac ggtgttgagg tcggcggcga aggtgtcgta gtcgtaaccg gtggtgggcc
     4321 ggctggaacg gccgaagccg cggcggtcgt aggtgatgac gcggtagccg gccttcagca
     4381 gggctgcggc ctgcttctcc caggactggc cgtcgagcgg atagccgtgg atcagcacga
     4441 ccggctgccc ggtgccgtgg tcctcgtagt agaggtcgat ggaggtggag ttctcctggc
     4501 cgacggtgat gaatgccatg atgctacttc cttcctatat gaggggtagt gccgtgtatg
     4561 ggatgtatag gtgtgaataa ggggatggac gcggggtgtg gtcagcgggc cgtgcgggcg
     4621 gccttctcga cgatccgggt gacggcgtcg gggcgtgaga cggtcaccgc gtgggaggcg
     4681 tcgatctcgg tcgtgtgcgc gtgcgcccgg gccgacatga agcgctgggc ggccacgggg
     4741 atgttccggt ccttggtggt gaccagcgac caggagggaa tggccttcca ggcggccgtc
     4801 gtggacttct cctccagcgc ggccgcggcc accggacgct gtccggcggc catgagccgc
     4861 gccttgtccg ccggcacgtc cgcggcgaac tgcttgcgga acttctccgg cttgatgtac
     4921 acgtcggccc cttggccgcc gtccggcagc gtgtagttca ccggctcgat gacctgaccg
     4981 agggtgctgc ccgggaactt gtcggtcagc cccagcgaac tctcgcccgc gtcgggcagg
     5041 aacgccgcga tgtagacgag cgccttcacc ttgtcctcca gtccggccgc ggcctggctg
     5101 atgacggtgc cgccgtagga gtggccgacc aggaccacgg gcccgtcgat gtgatcgacc
     5161 acgctgcgca ggtaggcggc gtcgttcgcc gggccgcgca gcgggttcgc ggcggccacc
     5221 accgggtagc cggaccgctg cagccgcttg acgacgccgt cccagctcgc ggcatcggcg
     5281 aacgccccgt gctccagcac gatcgtcggc agcgccttgt ggcggggcga ggctcccgcc
     5341 gccccgggga gggcgacggt cacggtggac agggccgcga gggcggtgag accggcaagg
     5401 gtggtccggc ggctcgggcg ggtggagcgc atcgtggaag tcctcttcct tgggttgtct
     5461 gtcgtgggtg gcaaggcgct gatgagctgt gtggggacca caccgaaacg caccggggcg
     5521 caccggggcg caccggggcg cggccagagg tggttgccag gctgaacagt tccggtcctc
     5581 ataccgctct ccggggccaa aggcctcgct gacggcgcct tcttcgtctc ctacgttccg
     5641 aggccaggtc cggttcaccc gtgttgcacg gaccgggatc agcgggacgc cgaggccggt
     5701 ggggtctgac ccggtcaggg ccgcggcagg agtgacaagg gacggcgaca agaagacgaa
     5761 aaggtttcgc caaggcggtc cgcacagtgt tggccccacg ctcccgctcg tgtgggggca
     5821 cgccacagaa cacgtgaaca ggatcgagcg ccacctatga acgggacctt ggacgcaagg
     5881 gtcaccacct ccgcccccgc ccggatgcgg gcccggcaca tctcatcgga cgtcgactgc
     5941 gacgccttca tcgaagagat ctacgaccag tacggtccct tgctcgtccg atacgccaca
     6001 cggctgctgg acggcgactg gcacagggcg gaggacatga tccaggaagc cgcgacccgc
     6061 gcctggaagc acgcccggtt cctgggcgcc cgccgcgaac atgtccggcc gtggctgtac
     6121 accgtggtca agaacctggt catcgaccac catcgcgccc gccgatgccg ccccttggaa
     6181 caggtccctg cccaagagct ggacgccgtt tgggacggct tggagaccac gctcacctcc
     6241 cacgtgatcc tcgaagcgct gaaggaactc aacgaacagc aacgagccgt cattcacctc
     6301 atgtactaca tggaatgcag cgtggcccag gccgccgaat acctcggtat cccacccggc
     6361 accgtcaaaa gccgcgcctt ctacgccata cgcgccctgc gcacaacact cgcggaacgg
     6421 ggcgtggtcg gccaatagcc tcccccggct ccttcgacct gcggccccgg caggaccagg
     6481 gaacgccgag tatccatcag gagcccggga gggggcatac cgcaagactc tggggcggcc
     6541 ggttctcagc cggtcacggc tatccggaga ctcccgcccg gcgcatgctc gctttcgacg
     6601 ccgcctccct cagtagccct gatcgtgctg gctcggggag gccgacccgg cctgccttgg
     6661 gtgccccgct gaagcccgtc ggctgtctgg ctgctgctcc ccagccgcct tcaccggtgg
     6721 agggtggtgc gctgtcgcgt ccctgaccgg tgtggctgac cgtccgcgtc gacgagcttc
     6781 accgcgtcca gcaccactgt cgacggccgg gtggacggct gggaagtacc gaccgccgtc
     6841 accgggtctt cactgcggtt gcggtccaag gcccgcgtcg cgggcccgga cgatcgccgc
     6901 ggcacggtcg tggacttgga gcttggagaa gatggcaccg acgtgattgc gcacggtctt
     6961 ctccaccacg cagagccgct gcgcgatctg tcggtaggtg aggccggccg cgagcagatc
     7021 gaggatgtcg cgttctcggg ccgtgagcgt cgggaacgcc tccttcgccg gtgtgcggcc
     7081 gaaagcagcg aacagtgcgc tcaaccgttc cgtcatctgc gggccgaaga ccgctccgcc
     7141 ccgcgcgacc gtgtgcaggg cgcgtaggac ttcctcacgc agagctccct tcaggaggta
     7201 gccatgagca ccggcgcgca tcgccgcgag gaggctgtcg tcgtcctcgg acatcgtcat
     7261 caccatgatg cgaaggtgtg ggtgggccgt gagcagtcgg cgtgtggcct cgatgcccga
     7321 gataccggga aggcagaggt ccatgaccac caggtcggga tcggtgcgtg cggtttcggt
     7381 gagtgccgct tcgccgctgg ccgcctccgc gaccacggtg aagtggtcat ccaggtcgat
     7441 cgcggctgcg aggccttccc ggaacagggg gtggtcatcc accagcagta ctcgccgagc
     7501 cggtgccggt gccggtgccg gtgccggtgc cgcatgatcc tgccggtccg cgtcgttctg
     7561 ggcgctcatg accctcctca agttctcggc cctgccgggt gaggggcaag gtggcgtgca
     7621 cggcggtgcc atgagggccc gtgtggacgc cagtcgtgcc gccgatctcc tcggcccgct
     7681 ctgtgaacga ggcgaggccc gtacccggta cgcccccgcc gggcagaccg ttgccgtcgt
     7741 cactgacctt gacggtcagc gacgcgggtg tgacagccag gccgacgagt acgcggcggg
     7801 ctcccgaatg acgcacggcg ttggtcatcg cctcggcggc gatgcggtag gcggccgtct
     7861 ccaccgcggg ggccagaaca ggcatcttcg tcgcgggatc ggccacgacc acctgaacca
     7921 cctcggtact caaccggtgc gccagaatcc ggatcgcttc gaggagacca cgctccacca
     7981 gcacagctgg tgtcaggccg gcggtgatgc ggcgtacttc ggtcagtgcc tcactgagtc
     8041 cctcggcggc tgccctcaga tgccgcggcg ccgacgactg cggcccgacg cacgcctgag
     8101 cggtgcccaa cctcatctgt acggcagcca gttgaggtcc caggccgtca tggatctcac
     8161 ggcgcagccg acggcgttcc tcttcacggg ccagtacgag gctttcccgg gcggcctgcg
     8221 tctcctcgac cagccgcagc gccgccaggg cgggtcccgc ctggtcggcg agcagggaca
     8281 gcaggtcgct gtccctctcc acaggagtgc tcgtgccgtc gcgcgccact tccaggcgac
     8341 ccactacctg gccgtggtgg cgaagagcga acacctgttt aggctcgatg accggcactc
     8401 cgaccgcgcc gagcctgcgt acacctgacc gggtgtcgac ctcgaccgct gctcctgaca
     8461 cgcccaggtc ctccaccgca ctgcggcaga tctgctccgg cacatcccgg gggtcgggag
     8521 cctcctgcaa tcgcgtcacc agttttctca tggcgtggtg gggcctggct ctcgaaccgt
     8581 agaagaagcg ctcgactcgg agagacgact gcaccaccac gggccgggtg acccaaccgg
     8641 ccagcagcgc gcaacccgcg acggtcaggg acgccgtact gcgtacggcc ggaagagccg
     8701 cccaggcgac caaggccgcg ccgaccgccg cggtgccgaa gacggtgagg acgaacgcca
     8761 cggacaatcg gtggacagtc gcacgttgca gtcgccagac atcgcttctg gcgacggtga
     8821 ggcagatgac gacgacccag accgtgcctc ctccggcacg ggcggccatg gtccagcgtt
     8881 ctggccacca ctggccgagc agcaaggaca cacagacggc gtaggcgacg gcaagcagaa
     8941 gccagcgacg gcgctccgcc gcgtcgtcac cggacagcga gcagtgggcc acggcggccg
     9001 tcacggcgag cgccgccgca ccactcaccc agtccaccag atggctcacc gggccgatgt
     9061 gctgcagggc ccactgcgcc gtcgcggtcc ccgaccacgg actgaagaag ggcggcgccc
     9121 ccggcacccc gaggaaccac agagccccat ggccggcgtg ggcagcggcg gccggcgcga
     9181 tcacgtacca gcgacaccgt cgcacggcgg gttcgcgcag gaaacacacg ggaaagacga
     9241 ggacgctcag ggggtggccg gcgagagcga cagccgccag cgccagaacc gtcacatcca
     9301 ccggcgccga ccagtcgccg atgaaggcga gcaggtcgga caccgtacgc ggcacaacca
     9361 gcacgactcc tgcggccagc agcagcgccc ccatgcctcg accggaacga tcggcgagga
     9421 gcagcacccc gatcacccac accggaagcc ccaccgccaa ccgcatgacc tcagcgatgg
     9481 tctgcggcgg tgagtagtgg acagcgcgcc actgctctcc ggacacggac agcaccgtca
     9541 gtttcaccac cagcactgcc gcaagcaggc ccagcgccat tccggccgcc cgtgccacgg
     9601 gcttggcgtg cgggtggccg gagacggcga ccgaccccgg cctcaagaca cccacaggcc
     9661 ccacggccct ggcccgtccc aggagggcag gcaccggtag gtggcccact tgccctggcg
     9721 cttgccccgg gctcccgcgt caagacaccc cgacttggag tggtagtgcg cgatcagccg
     9781 ctcctcggcc gcagcctggg caggggacgt cgagacggct gtcatggcga tgatcacgcc
     9841 cagcacagca gcgaacgacc tcatggaatt ccacatcatc actctcgttc ctccaccttc
     9901 agcggcggca cgcgttcgcg ttccgtatga ggaagaacga ctgatcggcc gttgtgtttc
     9961 acgtgttggc ccgcaagggc ggacacgtgc cggaacccct tcagcggccg gcccgcacga
    10021 cgatgacgga atgaaacgga acgagagagg cattgctctc tccgaccggt tcgccgtccg
    10081 ccaccaccca cgcgtgcgcg cggaagggct ccgcgcatac cccggtgtgc cagtccggcc
    10141 atgtcccgcc ggcacggcac agaagggctg tggcgatgga gcgaggcaag caccacgggc
    10201 cggcgcagcg cacactggcg gcaaccaccg ctttgcgggc acgcagcgcc tgctcatgcc
    10261 ctgcgggacg ggcccggacg ctgatgaggc gcaggacccg ccgtaggccg ccgggcgaga
    10321 gacgggcgag accgaacgcc acgacgactg cgcaccgtgc ggtgaggagg agaccgaagg
    10381 gtgggcgctc ggacacttcg gtggtgacca ggccactcat gagatcacca ggccggcatc
    10441 acgcaggccg tcgatcatgg cccgtacgtc gcgcagtgcc cgctcgtatg ccttgggatc
    10501 gccggggcgc agggcagccg cggcggtttc ggccgtcccg ccgtcgagca ggcaccgcag
    10561 cacccgcgca ccggcgtcgt tcgcctgcca gtaccggccg gtcttctcgt tcaggagcac
    10621 cgcgccgtgc tcggtctcag tgttggtgac atgcggggga agggcgaagc gcatcagatt
    10681 tcctcgcggt tcggtgggag ggacgcagtc gtgccactcc ggttgtcccg gtacagggat
    10741 cgcagccaga gctcgcatgc cagcgtcgaa tcgaaggcca tgaaggggct ggtgtctggg
    10801 tgcaggccga ggagcagtcc cctcagtgcc acgcggtcga ccagacccat ccccacgagc
    10861 acggactcct cgcacagttc gaggagctcg gcccggtgct ggcgcaggcc gctgtacagc
    10921 tcaccgctgt agtcgccctt ggtcgcgcgg ccttgcgcgt acgccggaag aacgtctcgc
    10981 atggcggtcg caagcaccgg tttgtagcgt ccggccgcga tccggtcctc cagccggacc
    11041 gacatcgcgg cctcgacgac acggtcgtcg aggtaagggg cttcgtagga gaccccgtga
    11101 cgtgcggtga agcgggaggc ccggcgtgtc atctcaccac agagctgagc gacgctcagc
    11161 atgtcgtgct gcaccggcag cggggcgagc ggctccggct ccccgtccgc cgccgcgcgg
    11221 atgaggctgc gtacggcgtc gaccgctcgg ggcgttgccc acaccggcat ggcgggcggg
    11281 ctttcccacg ccggccccgt ggcccgcttc ctcggctccg ggccgaggag ggcggcagag
    11341 gaccggagcc agcgggcata ggtgccaccg gacaggagat agccgatagt ggcggacgtc
    11401 gtccagcggc tcatactcct gacgacccgc acctgccgcg ccgatcggag cgggtgtgcg
    11461 cgagccagcg cgtgcgcaca tgcggcacgg gcgtggaaca gctcgtcccc tccgatgccc
    11521 tgaaggtgcg ctgtcgaacc cagcgcggcc atcgttcggg ccagatgttc cagtttcgcc
    11581 cgggtccgga tgagaggaaa ggggccctcc acgtcctcgt cggtggacag acacccggca
    11641 taccaggccg gcgccgcgcc ggaatgctct ttgacatgcc gtgcgtcagg cagatcggct
    11701 gcgcaccgac ctgcccagat gccgtcgtcg ttcgacggat ccaagggcac atggtggtag
    11761 gtgaccagcc gggcagctcg tggggcggcg aggaagcaca gactggtgga gtccatgccg
    11821 ccagagagat cagtgctcac cgtggcggag tgagcggtcc gcgtctcgac tgccgcctcg
    11881 agcgcccggc gcagtgtgtc ggcgccttgg gccagcggaa cgctcggttc gggtggcgtc
    11941 caccaccgga cggggcgcgc atcgctgttg gcgggcatca gcaggtaatg gcccggcggc
    12001 aaggcggtga cccctcgcca caggcaggcc tgggacaaag gatgcggcgc ggtgggagag
    12061 aggaggcgaa gtgccacgag ttcctcatcg atggtggccc cggtcagcgc ggccacactc
    12121 tggggccggt cggcggccac cgccaccccg gccacggatc cgtagaagac ctggcacacc
    12181 gtggtcaggc tgccttgagc cctgacctgg cccccgatgg aggcgagcag gtggaagcat
    12241 ccgtgcgtcg agcggctcaa cgagtccatg tcggtcactg agcgcatcac cgcgagcttg
    12301 cgggcgagtt cggtttcgcc gatgcgggtc cgaccgagca ggaccaccgc attggagccg
    12361 gcggaggcgg cggtgagttc ctcgtcgcac caactcccga tgacccaggg ccggcccgag
    12421 gggtgtctga ccaccttgtc cccggccctg cgcggcgaca gagcgagcac ggcatcagca
    12481 gcggggtgat ccgggagaac gatgaattcc atggggttcc ttgtcccaga gtgggcgatc
    12541 gacgcgaagc ctcgcgccga tcgcccggtg ttctgctacg tgccggaccg tcgcatcaga
    12601 gcgcggtctc gatcacagcc accagccacc gaagaagtcg atccagtggc cggggcgaac
    12661 ggaacgggtc agctcacgga agccgccgac ctccacgatc agcggcatgt cgtacacgag
    12721 cttttcggac tgcatgggag tacctctttc ttctcgtcgt ctgcggtgga cgaggtctgc
    12781 cgtccatccg ccgcccgtct gatccggaca agaagagcat cgacttcccg ctgacccagg
    12841 cacatgagcc atacgccatg accgtcccgg aacgcgccgg gacggcagcc ccctcctcag
    12901 cccggctcat acggtgagcg gtacgccccg caccgaactc agcagaacat cgcggccgcc
    12961 gtcatcgtca ccggcagcgg accatcacgc tcccggcagt accgccggac catcccgccg
    13021 gccaccggcg tcggtatgcc aatgccgcag tcggcacaca tggggcggcg gggctcccgc
    13081 tcgccctgtg ctgggcggac caccgcaccg ggtgcacacc atcgggtacg cgccatcggg
    13141 tgcgctcagt ggccggtttc ccggcctcgg tatggtccgg accctgtggg tggtgcgcct
    13201 gccgaccgtc cgggcgcacc ggccggtaac gccgtcctgg ccggtatagc ggtccgcgcg
    13261 gacacgggag tcgtcgcccc cttggcgaac gcgtcctgga ggcggcgcag ccggtcggcc
    13321 cgagcgccgt cggatccagc gcctcgcggc ggccgggctc ctgcttctgg tcgtcgggct
    13381 cagggtcggt ggccgggggc gcgagcagtt gctcgggcat cgtcgcctgc tggtggacgg
    13441 cgacctacgc ccagggcatc tcccactccc ccgtggcctg gtccgaagcc cggggcgcgg
    13501 ccgactctcc gggatcggac aggagttgcc gctccggatc gagcgcggga gtgggtgggc
    13561 ggttccaagc ggggaggtcg ggcaggggcc aacgggtggt gcaggcgatg accgcgctcc
    13621 aggccgcgta cggccttcgg ttcgctgatg tccaggcagt gggtcgagat ggcggcagcc
    13681 acgacctggt catccaggcc ggggtcgcgg tggtggccgc gcaggacgag ggcaaggtgg
    13741 cgtcgggctt cggcgagccg gtgccgacgg tgtcagcgcc cgccctcgtc catcaggaac
    13801 accgtcgcag tgacgtcgac ggccgccagg cgacatcgac cacggcggcg gcccgggccc
    13861 ggatcgcggt agccgcggcg cgggcgtggt cgaggaggga gaggatgacg tcgacagcga
    13921 ctccggaggt gaggatcgcg ctcttcttcc accatgcgcg cagctgcgcg agcgggtcgt
    13981 acgggcggcc acggcgttca gcttggcgcg agcccgctcg gagagcacgg gcaggaaacg
    14041 cggctcgccg tcgtcgacgg cggtgacgta ctcgtgctcc agcccggcca ggcaggcggc
    14101 ggtctggtcg ctgcgccggg cagtccagcg gatcagctca tgcggcaccc cggcgatctc
    14161 catcaccggc aggcgccccg gggtgacgat gcgcggctcg gttgccaacc cgaggtccgc
    14221 gaaggcggcc tgtgttctca tgcagggccg tggtgtggat cgagttgtcg gccctcattg
    14281 ggaatgttcc gtcccgccgc aggcctgagc tgttcgcggc tgtctcgttc cgggacacgc
    14341 cttggggctc acgatcttgt cctcagaagc gacgccaggt ccgggaatcg gcgagatacg
    14401 ccccacggcc ggcagaaggc cggggcctct ggtcgacggc atacccggtg tgggtgtgcc
    14461 gtcgaccaga ggcgtcggct cagagcagtg cgtgcggata ctgttcggcg cggtgctgga
    14521 aggccaggac tgcggggttc tggacggtgc cgccgcggat ctcgatcgct cggtgaatcg
    14581 tgcggttacc gtcccagccg gccggtcctt cgagaaccgg ccgcaggaag gggatcagtg
    14641 cctcactgtt ctcccaggtg acggcatccc acaggtagga gggactgtgg tccacgccgt
    14701 agtagtggac gtggccgccg acggtgaaca tcggctcggt gaaggtggta ggccgcgccc
    14761 aggcgaagcc catgccctgg tcgcaggaga cgtcgatgac gagggtgccg ggggcgagtt
    14821 tcggcaggtc ctcctccagc aagaacatca gcggcgccgc ggtgtcctgg agcacgcagt
    14881 tgatgatgat gtcgtgtccg gcgaggaagt cggccagcgg ctccgggccg tgctcggtga
    14941 gcacgacgct gcgccggggg tcgagggtct cgtcggccac gtcgtggtcg aagtgcacga
    15001 tcctcgcgga gtggatcggt gaactgaccg cggcgacccc gcgggcggtc aacacgtcga
    15061 cgtcatgcac gcccagcgca ctgagcgcgg tcaccgcgcc gcgggcggtg gcgccgaagc
    15121 cgatcaccgc cgcacgccgt cggcgtccgt agtcaccggt cgctccggtc agctgcatgg
    15181 catgcagcac cgacgagtag ccggccagtt cgttgttctt gtggaagacg tgcaggttga
    15241 acgaaccctc ccgggtccag tggttcatcg cctcgaaggc gatcagtgtg agcctgcggt
    15301 cgatggcggc ctgcgtgacc ttctcgtcct gcacgcagtg cggccacccc cacagcacct
    15361 gtccttcccg cagctccgca aggtcctcgt gcagcggttt ggtcagcagg atgacgtcac
    15421 actccgcgat cagctcctcg cgagagcgga aaccggcgac ccatggtgcg agttgccggt
    15481 ccgacacacc gaagtgctct ccgtaaccgg cctgtaggta gatgctgggc tgaaggtcgg
    15541 cgtcgatgcg ttcgaagtgc gctgggtgga tcggcagacg gcgttcgttc tctttgcggg
    15601 tctgcgaaat gattccgagc ttgagctgct gcaaaatacc cctctgattg ctacctgtgt
    15661 aacacaccct gcagccaagg ggcttgatcg gcagtgctcc tatgggtccg cgctacggtg
    15721 cggacgtcgg ccgacgacgg ccccgggccc tgtgagggag ggccgcgcgc tgcgcgtcgt
    15781 gccgcaaggg cctcccggag cgcgcctcgt ggtggcggaa gcgcgcgacg accgggccgt
    15841 ggggcggcca cacccggtgg atgccgtcat tgcggtgccg gatcacctcc acctcaatcc
    15901 actccaacat ccgctcgacc gcgtcctcgt gcgcgtggcc tcgatcaccc gccgcgtctc
    15961 ctcttcccct agggcgtttc gtttggatca gtcggtcgtt ggtccgggtg tgccgttgac
    16021 tgatgcgcag tgggcgcgga tcgagccgtt gctcccggac cggacgccga ggcggggtgg
    16081 ccgctggcgt gaccatcgtg aggtgatcga cgcgctcgcc ttcaagttcc agaccggtac
    16141 ccagtgggtg catctgccgg agaagtacgg caactggcgc ggcgtctaca accgactgcg
    16201 gatgtgggcc gtcgacggca cgtgggagcg ggtgttcacc gccctggtgg ctcaggccga
    16261 cgcggacgaa gacgtcagct gggccgtctc cgtcgactcc accatcgtgc gcgctcacca
    16321 gcacgcggcc ggggcccgca aaaaggggcc ccggccggcg agccggaaga ccacgccatc
    16381 ggccggtccc gcggcggact gaccacgaag atccacctcg cggccgacgg ccggtgccgg
    16441 ccgctggcgt tcgtcctcac cgccggtcag gccggagacg cacccgcctt caccgacgtc
    16501 atggcccgcc tgcgcgttcc ccgccgacga ggacggcccc gcaccaggcc ggacctggtc
    16561 ctggccgaca aggcgtactc ctcccgcgcg atccgcgacc acctgcgccg acgcggcatc
    16621 cgcgcggtga tcccagaacg ggccgaccag caggccaacc ggcgccagcg cggacagacg
    16681 ggcggcagac caccggcctt cgaccgtgaa gcgtacaaac agcgcaacac cgtcgagcgg
    16741 tgcatcaacc gcctgaagca gtggcgcggc atcgccaccc gctacgagaa aaccgcgacc
    16801 gtctacctcg ccggacttca catcgcgggc atcttcctct ggtccgcccg ctgatccaaa
    16861 cgaaaccgcc tagtgccaca gcaggcagat cgtcggctgc ggccggaaca cgaagccgac
    16921 accggtcaca ccggtcaccg tcacccggcg tccgagagcc ccggccttga gcgccttctg
    16981 cggggagtcg cccgccgtcg agccggtcca ccaccacatc gccggcgacc tcacctcgcc
    17041 tacgagggcc tggagatggc cgccgccccc ggtctcacac tcaccctgtt ctgaacgtag
    17101 caacgggcac cgacccggcc gggctttgtt ggcccgccgg tgggcatcga gagagctccg
    17161 aggttttcga gggatcagcg ccgacgagtt catctcgccc agcgccgtgg tggccaactc
    17221 cgtgtacctc cacaggcgat ggggggcctg gcccgcgtcg cagaagaaac cgccaccgta
    17281 gagccggact acggggacca ggccggggtg cagtcccccg tcgcctgacc cggggaaccg
    17341 acctacggct ggagtggtcg caaggcgccg gcgatgacag tggtcaccat cttgcggatg
    17401 gcgtcgggct gcggtggggc gttcgtccgg tcggcgaaca gcaggtgccc ggccccgacg
    17461 agggtggggg cgagtgtgtc gatatcggcg tcaccggcca gacgtcccat tccccgctcg
    17521 gcggtcaggt acgccgcgat catggcggtg ccctgcatga ccaatgggat gccggtcaga
    17581 ccggcttcgc gcagccgggc gcggaggctg tcccggaaag tgatgagcgc gacgatcgcc
    17641 accgtgaccg gcccgaacag ttcctgcagc gcgtcggcga ggttgtccac cacgccgccg
    17701 gtgccggcgg ccgcgcgtag agtcgtggcg tggtcctcga tgccgtggac gcggtcgagg
    17761 accagttcca ccaggaaagc gtcgaagtcg gtgaagtgcc ggtgcagcac gcccttggca
    17821 cagcccgcct cctccgtgac cgcccgactg gtcaacgcgt caggcccgcc ccgaagcagg
    17881 acacgctcgg cggcttcgaa caactggccg cgcacattgt ggatgtgcac ccctgacggc
    17941 attgtctccc cttcgaaccc gctccggctt aacaagtggg cgtccgccca ctagggtggg
    18001 cgcatgccca ctttatcgca ggggcagccg gacgcatccg gccccgagcc ccaccgtcac
    18061 cggcagacag ccgagtcgtt cggagtggac gccgaacgct acgaccgcgc ccgaccgccg
    18121 tatccccagg ccctggtaga caggatcgtc accgcgagcc ccggcctgca cgtcctcgac
    18181 gtcggagccg gtaccggcat cgaggcccgg caattccagg cagccggctg cacggtgctc
    18241 ggtgtcgaac ccgacgagcg gatggccgcc ttcgcacgac gcagccacat cgaggtcgaa
    18301 gtcgccaggt tcgaggactg gcagccggcc ggccggcagt tcgacgcggt catcgccggc
    18361 accgcatggc actgggtgga ccccgtcgcc ggcgcggcca aggctgccca ggtactcggg
    18421 cccggcggcc gactcgcccc tttccaccac gtcccccaac ttccggttga gctgatcgac
    18481 accctcgccg aagcacttca acgggtcgcc ccctattctc cgttcaaccc gggcctgctg
    18541 aagggatcgg ccgtggacgc ctatcagcca ctgttcacga agatcgccga cgggatccgg
    18601 cagaccggca gcttcaccga gcccgaacag tggcgcttca cgtgggaacg cacctactcc
    18661 cgagaacagt ggctcgacca gctccccacc ttcggcggcc tcacccaact ccccgcagac
    18721 aaattggcga aggtcctgga cagcgccggg accgcgatcg acaagctcgg cggccacgcc
    18781 accatgccct acacgtccat cgtgatcacc tcgacccgct cccacacggt ctgatcgccg
    18841 caaacgtcgg gcgtcgccgg gcacgcgcaa gcccatgagc tcggcgacac ccccggggag
    18901 gcagcggggt tcgagcacct ggccggcatc agcatgccgg gcccagggcc accactgggc
    18961 ggccgcgggt gtctgtccag caggccgggt gtgggatcaa ctccatgccg tcctgctgac
    19021 gtaactgccg gtgacgaagt aactgggctg gtcccggacg gtgatcgcct tcagccacct
    19081 acgggccgct cggcggggta cagaagggga ccgagcccgg tcggccgcgc acgtccaggt
    19141 agcacgcacc acgtcctggg cgacgggcaa agcatcccat tcgcgacgcg ctgaccggcg
    19201 gttccatcca tcccgggcct ggtcgaacgc ccgcgacggc gcacctggaa ccctgctcgg
    19261 cgaccgcaga tccggccacg aaaagcatcg ctgccgcgtc cgggcgcaga gcatcgaacc
    19321 gagcactgcc gccgccgcat tcccccaagg acccgggccc ggcgtccgcc tgtggaggat
    19381 cggcatgcgc ccgcgtgcag cagccccagg cgccaggcct gcccctgccc ctgcccctgc
    19441 ccctgcccct gcccctgccc ccgggcaatc cggggcgggc ccttcctatt ccgggccttc
    19501 cgcgtcggat gcagcacggc tggccagtcg ttcgtagcgc tgcctggctg cttgcggagt
    19561 gccgagcccg aggccgaagg cgatctcttg ccacgtcatg ccgcggccac gtgccatctg
    19621 gagaagtccg gtttcgagtt cgtccatctc gccgcgaacc atcgggacaa ggctcagtgc
    19681 ggcggtgatg tcggcgtggt ccacctccgg ctcagcgtcg tcgagctgtg cggccccact
    19741 gagcaggaac gacacgagtc tgacggcttc gtgcggcccg atgaccgagg ggtgaacctg
    19801 tcggtggcgt tgctcgtcgg tagccgcgtg ccgctcggcg atgcggaaca gggacgcgtg
    19861 tgcgcggtgc actcgcgcct gctccggctg cgggggcgtg aacgggtcag tgggctgttc
    19921 aggagtcgac gacatgagaa gaggatgagg catttcatga atcgttgtca acggaccgtt
    19981 ttaaacgact cgttcaatcg tgagcacggg aacttctgcg gcgcggaaac ttcctgactc
    20041 tcggccgttg cggcggggcg ggtgttggcc atgcaccagg gcaggattcg gctaggtgct
    20101 gggcgttctc ggggagatcc agtcggccga ccgcgccgcg cacttgcccc gaaaccaccc
    20161 tgctccactg atccagcccg gcgtgacgcc gccgattcgc cgccgcattc cttctccgca
    20221 tccggtctac aggtcagggt ccctgatccg gctgagctgt cggtcgctag ctctgggaca
    20281 acgcggaggt cgtgcgccgc tcgcgagttg tggcttggcg ggcgcggcgg ctggcgacgt
    20341 tgatgcggtt gccccacagg ccgtgcggtc cgggaatcag ggcggcgtcc ccctgcgttc
    20401 gactaacagc cccccgtagg cgcctgcttc gaccaggaga actcatgtca gagttagcga
    20461 tcgatgccat cgtgttcgac gtgctcggta cgctcgtcga cgagcccgcc ggtattcgcg
    20521 ccggtattcg tgaactcgac ccatcgctcg acgatcctgg ggtcgagcag cttttgtcac
    20581 tgtggcaaca gcacatcgac cgcgagcaac gtcgcgtcct cgacggtgtg cggccctacc
    20641 tcaccagcga cgtcctcgac ctggaagccg cccggctcgt cgccgatgcc gccggagtcg
    20701 acgacccggc cgccgtcgcg gcgctggcta tgtcaggtcg caggctcccg ccgtggcctg
    20761 acaccgtggc ggggctttcc cgactggccg aacggtttcc actgatcgga ctttccaacg
    20821 cgagccggac ggcgctgctg gggctcaacg cccacgcggg actacgctgg caccaggccc
    20881 tgtccgccga agaagcccgg acctacaagc cggacccggc ggtctaccag ttggccgtca
    20941 ccgtctccgg acgaccgccg gagcggctcc tgatggtcgc tgcccacgcc tgggacctgc
    21001 gcggagcgca gagccttggc ctgcgcaccg cttacgtcgc tcgccccgtc ggcgacccgc
    21061 ccactccctc ggacggattc gacgtgcacg ccgacgatct ggccgggctg gctgcacagc
    21121 tcgaccatcc ctaggagcac cgacgggtca tcggctgtgc cagagtcggg gcgttgatcg
    21181 tttgctcaac ttcgttcctg tgttggcaga tcgttctggc gagccggtgg gccgacggcc
    21241 gctgctggca cggcgttgac gctcgcgcgc tggttgcccg gctctgggat ggtgtgcttg
    21301 atctggcgtc ttcgtaggtg gcggcggttg cggcggaagg ggtacgcctc ttctcccaaa
    21361 aaacctgtct cgtaatgacc tcaagagggc cgacacgccc ccctcgatct cgcgcgcgca
    21421 tacgcgggcc ggttgtgcag ggcactcgta gagggggagc cggagaggtc agcctggccg
    21481 aagtcgaccg tgtgtccatt ggcagaccgc gtcccgcgtc cgccgccgtc acagcgccgg
    21541 gccaagccgg tctgcttatc cgggtcttgt ctctccgaac acggtggttg cccgggaact
    21601 cggccaccgc gatggggtcc tggcggcatc cctccaggac cccgcacagt cagcgccgat
    21661 cgtgattcgg gagcgcgatc ctgaacctgg ctatggtgcc cgcggtcgtc caccggtgga
    21721 agccggcttt ctccagtact cggaccgagg ccgggttcga cagttccacg tctgctgaca
    21781 cggtgtggac gtccggcgcg gtgagggcga acgcggccag ggcctgagtg gcctcggggg
    21841 cgtaaccccg gccgcggcgg gaggccacga tgccgtatcc gatctcgagg acgccgtcct
    21901 tgggcggcca gaacaggccg atggagccca ccaccagacc gctgtcgcgc tcaatgatca
    21961 ggcgatggcc gtaggcgccg agccagtcgg ggtactgggt gaagaggcct gcgatcacgc
    22021 ggtcgccttc ggcgggaaag tcgtccgccc agtgagggga gcgggcgccg ccgaggaccg
    22081 cggtggccgc gctcttggtc caggaccgca gtacgaggcg cgcggtgacc acgtcggtgt
    22141 gggcgggtgc agaaggggaa gacatgtgta tctcctggtc gttggaacga ccggccgcgc
    22201 tctgttatcg cacgggccgg acaagggcat gccgatgaag gccgacggcg gccatattca
    22261 tcaacctcct catcccgaga tcacgcgtct gtcacgctgg cgcgagggta gtagccactt
    22321 tccggccagg gcaacacatt tccgggacgc tgcccgaggc cttcactcgc tgaagggtgt
    22381 gcctccggcg agtgacggct cgttgctctg gtcatgcaca gcagaggggc tacagcccgt
    22441 ggcagaccat ccactcaggc cgcccttccc cacgccgtag ctcagtcgat ggccgcgtca
    22501 cccgaggagt gc
//
