LOCUS       NZ_CP058561            11827 bp    DNA     circular CON 15-DEC-2024
DEFINITION  Vallitalea guaymasensis strain Ra1766G1 chromosome, complete genome.
ACCESSION   NZ_CP058561
VERSION     NZ_CP058561.1
KEYWORDS    RefSeq.
SOURCE      Vallitalea guaymasensis
  ORGANISM  Vallitalea guaymasensis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Vallitaleaceae; Vallitalea.
REFERENCE   1  (bases 1 to 5626074)
  AUTHORS   Postec,A.
  TITLE     Vallitalea guaymasensis genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5626074)
  AUTHORS   Postec,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2020) Microbiologie Environnementale et
            Biotechnologie (MEB), Mediterranean Institute of Oceanography (MIO),
            UMR 7294, 163 avenue de Luminy, Marseille 13288, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JUNE-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141425.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 15:20:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,781
            Genes (coding)                    :: 4,757
            CDSs (with protein)               :: 4,757
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 16 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:55:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 847752
            Orig. end                         :: 859579
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058561.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11827
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11827
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11827
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6827](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6827
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(968..1645)
                     /locus_tag="HYG85_RS03795"
                     /old_locus_tag="HYG85_03800"
     CDS             complement(968..1645)
                     /NRPS_PKS="Domain: ACPS (101-161). E-value: 1.3e-12. Score:
                     39.6. Matches aSDomain:
                     nrpspksdomains_HYG85_RS03795_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013786.5"
                     /locus_tag="HYG85_RS03795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03800"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_212692353.1"
                     /transl_table=11
                     /translation="MKALAIIKDIDVNNTERFNVSLMPKERQERVELFTDKKKIKRFLA
                     TEQAASQFTAISFGIPVSNFNGGIGEKPYLKDYPDISVSRSYAGEYIVIAAERSYNIGI
                     DCEAVQEFNNNIVKYFFTHRESEYIESSKDRNTAFTLLWTRKESFIKCIGRGIDYPINT
                     IDVTPRENIDNIHSDRPIFSENDKVNDYYINSYIFEKLVISVCSDNNDAFPILDHIYKG
                     AVR"
     aSDomain        complement(1163..1342)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYG85_RS03795_ACPS.1"
                     /evalue="1.30E-12"
                     /label="HYG85_RS03795_ACPS.1"
                     /locus_tag="HYG85_RS03795"
                     /protein_end="161"
                     /protein_start="101"
                     /score="39.6"
                     /tool="antismash"
                     /translation="IGIDCEAVQEFNNNIVKYFFTHRESEYIESSKDRNTAFTLLWTRK
                     ESFIKCIGRGIDYPI"
     gene            complement(1807..2463)
                     /locus_tag="HYG85_RS03800"
                     /old_locus_tag="HYG85_03805"
     CDS             complement(1807..2463)
                     /NRPS_PKS="Domain: Abhydrolase_1 (18-120). E-value: 2e-21.
                     Score: 68.6. Matches aSDomain:
                     nrpspksdomains_HYG85_RS03800_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF023568.5"
                     /locus_tag="HYG85_RS03800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03805"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_212692354.1"
                     /sec_met_domain="PF00561 (E-value: 9.7e-16, bitscore: 56.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIKVNEVTLFYQKYGSGNPLIMLHGNGETHEIFNRSISLLQEYF
                     TVYAIDFRDHGNSSKVSELHYEDHAKDIYEFINQLNIKNPVYYGFSDGGIVGLMLASEY
                     PNLFSKLIVSGPNINPKGLRICSRLMMKLMYIINKSQKVKMMLQEPNISLSDLSKIKVP
                     TYITGGSFDVITKKHLYLIHENVKNSILKIFKYHTHTNYIVDSEIIGKYIIEVCK"
     aSDomain        complement(2104..2409)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYG85_RS03800_Abhydrolase_1.1"
                     /evalue="2.00E-21"
                     /label="HYG85_RS03800_Abhydrolase_1.1"
                     /locus_tag="HYG85_RS03800"
                     /protein_end="120"
                     /protein_start="18"
                     /score="68.6"
                     /tool="antismash"
                     /translation="NPLIMLHGNGETHEIFNRSISLLQEYFTVYAIDFRDHGNSSKVSE
                     LHYEDHAKDIYEFINQLNIKNPVYYGFSDGGIVGLMLASEYPNLFSKLIVSGPNINP"
     gene            complement(2468..4063)
                     /locus_tag="HYG85_RS03805"
                     /old_locus_tag="HYG85_03810"
     CDS             complement(2468..4063)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="HYG85_RS03805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03810"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_212692355.1"
                     /transl_table=11
                     /translation="MKISKKRLGFFGIIVRNFIIFTVSVLAVLILAGIIGIAVIYFDFA
                     NYYSAHFEDSKEFFIEGNYDSFQRERLLGEDGFFTVFDDKNNVIYRSEGAESFNLTKKQ
                     VDLIPESEAGLTISIDEYVGQDDNKYINVAYETVDEEDNTIKSNIILDSNFNILFSDIP
                     NIEKRLSESDFNLLTGKYKDDINISKLSYTSKNGKIYNVVFCRNVDSDNLNTSMKVINY
                     IGIAYLVFFVVAVLFFVISLNKKVKEPLLLLGNAIKHIAEGKTDKIIKYKGPREFEEIC
                     NSFNDMALKLHETEKKRIGVEQEKQKMLADISHDLKTPITVIQGYAKAVADKKVNDNER
                     SEYLNTIYKKSNSLAELINTFSEYSKLERPDFKIQTVKTNISEFARNYLIDKYEELEFL
                     GFELDIQIPDEVIYCMLDEFQMKRVFENIISNTVKYNEKGTIISFVLERLDDKCIIRIG
                     DNGIGITDDLKKTIFNPFSMGDSSRSKLKGSGLGMAIVKKIVTAHRGTIELIQEKLEGL
                     SVVYEITFPLTEEE"
     gene            complement(4050..4742)
                     /locus_tag="HYG85_RS03810"
                     /old_locus_tag="HYG85_03815"
     CDS             complement(4050..4742)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598530.1"
                     /locus_tag="HYG85_RS03810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03815"
                     /product="response regulator transcription factor"
                     /protein_id="WP_212692356.1"
                     /transl_table=11
                     /translation="MHNTILVAEDNCEISKVIKLYLEVEGYEVLIADNGLDGLSLVKEN
                     KIDLAILDIMMPKMDGYELTREIRKISNIPVLILSAKNEDIDKILGLNMGADDYITKPF
                     NPLEIVARVNAALRRHYKLSGNEKNSGILNLGELNLDTNNCRLVKQEQEIQLTATEFKI
                     LALLMRVPERIFSKVQIIEHIKGEYFETDDNSVTVHISHLREKIGMNKNGNQYIKTVKG
                     LGYKIEDK"
     gene            5001..6827
                     /locus_tag="HYG85_RS03815"
                     /old_locus_tag="HYG85_03820"
     CDS             5001..6827
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /locus_tag="HYG85_RS03815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03820"
                     /product="YcaO-like family protein"
                     /protein_id="WP_212692357.1"
                     /sec_met_domain="YcaO (E-value: 5.1e-45, bitscore: 152.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLEHKKHFKEVSPTETVTKLKNILSELGIETEETWSEESFVETY
                     SLRLTFKGTTIGTNGKGITREYALASAYGELFERYQNNLLGIIISKKESGSDFYEAPDE
                     KIVTAEEIVSYDNSLMNLYFSNRSKKDTSIKEKAKAFKSTQKMDLLKHNLENRYIVVPF
                     YSMRDEKIVYLPRGTCRRFYGSNGMCAGNTPAEALVQGLSEIIERVVQKKLFLEKPTLP
                     DVPDDYIKRYPYIDKMYAKLKEQEDYYVTLKDCSFGGKYPVAALVIVEKNTGNYGVKLG
                     CHPDFGVAMERAFTEATQGNDIFTYAQRSHIDFSNLDSSNSMNIYNSYKLGVGQYPYQL
                     FGNKPTYEFVPLKDVSSMSNQEILNYWVTDIINDGYDVLIRDVSNLGFPSYYIVIPGLS
                     EMWEDKDVIFRAYNTRFFISPLVANPTKINRSNTKYIIATMEFFMNCVLENTMESYCKF
                     TEDWPCENFGCDTRFFVVMCHVINEEFSIAAEKMKYIVNMAQRASIPKDQFKFYKGMYN
                     YFVAMSQIHDHNKVLEYLRVFYSEDTCKKLDNLFCVPKQVMVKLFVPKSTDSPKLQEVK
                     DSYKQCQEIIHKLHSAQIKNPINQKDIYELFA"
     gene            6964..7155
                     /locus_tag="HYG85_RS03820"
                     /old_locus_tag="HYG85_03825"
     CDS             6964..7155
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HYG85_RS03820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HYG85_03825"
                     /product="hypothetical protein"
                     /protein_id="WP_113671765.1"
                     /transl_table=11
                     /translation="MKEQEKYGLFLDNELSSLTSSFDIRSEDTDDLPIAAAAAGPEGAG
                     AAAAAPEAFYAPGGQALA"
     gene            7371..8480
                     /locus_tag="HYG85_RS03825"
                     /old_locus_tag="HYG85_03830"
     CDS             7371..8480
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HYG85_RS03825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HYG85_03830"
                     /product="metalloprotease"
                     /protein_id="WP_212692358.1"
                     /transl_table=11
                     /translation="MQSPCINQNLELMPKDDGRIFIFNKSTKKTYSLGRNEYKVLSILD
                     GNKTMEEIHKKCNNYSLYDIEKLINEFSKIGLLKTEDTNQSPTDIIEKKVVDIKDYQKK
                     RFSIMDKKIFKIFSLKKGILHPDNSLDKDKMIFRILYFSIIYLSLPIFLIGLLVSIGTS
                     SLSNSLTAPSWYIFIPAFLISIFFHEFAHIIVAKNNGAHIAEVGIRLILIVPCIYSSIV
                     GISYIESKIKRILISAAGILLNLQVAGIGFILTAFTEGNANNFFLWLSMFNLMLVMFNL
                     IFLLKFDGYYILTELLNDKKLKENSLSFISYIVFGKLFRKATVTNMRIKLSLKKKIIYV
                     LYGTLNIAFVGCIFVTFITNIIRFISSKI"
     misc_feature    8619..8634
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.73"
                     /tool="antismash"
     gene            8722..9201
                     /locus_tag="HYG85_RS03830"
                     /old_locus_tag="HYG85_03835"
     CDS             8722..9201
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025701.5"
                     /locus_tag="HYG85_RS03830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03835"
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="WP_113671767.1"
                     /transl_table=11
                     /translation="MSKVFNVQKLFVLTAVFLMLGASTVFASSDYYLDTPTEIDFNYIE
                     ESVKDANKVMDMEFTVVDDDNVQDVIKQNIDILKFNPFKLSFRDGHYLYIAVGYGEQRT
                     GGYSIQVNELYESQDRIVISTELLCPGADDIVTMNITYPYLIIRTSDLFLPIYYK"
     gene            complement(9330..10202)
                     /locus_tag="HYG85_RS03835"
                     /old_locus_tag="HYG85_03840"
     CDS             complement(9330..10202)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF018184.5"
                     /locus_tag="HYG85_RS03835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03840"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_212692359.1"
                     /transl_table=11
                     /translation="MDLLDSMNNAMNYIEEHLAENISYEQVARRACCSEYHFQRMFPFI
                     AGITLAAYIRRRRLTMAAFELQNTDIKVVDVAMKYGYQSTEAFSRAFKNLHGVMPMLAR
                     KQGTALKAYPRMTFHISIKGDVEMDYRIERKEAFEMYGISKEINAVDNRQYIDIPAFWH
                     KCIENGKLDDMHKDFNLDQDECLHAALYNFREGILSYMICYYPPENVSLPAGYTKLSVP
                     ATTWAIFSTAECTQEGGTEAIQNIWKRIFPEWFPNSGYEHADGPEFEMYYNKGDGKFIE
                     EVWIPVVKK"
     gene            complement(10302..11420)
                     /locus_tag="HYG85_RS03840"
                     /old_locus_tag="HYG85_03845"
     CDS             complement(10302..11420)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444464.1"
                     /locus_tag="HYG85_RS03840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYG85_03845"
                     /product="aldo/keto reductase"
                     /protein_id="WP_212692360.1"
                     /transl_table=11
                     /translation="MIYKEYGNTGKKVSAVGFGGMRFDESRSLEENAELVRYANSKGIN
                     YFDTAPGYCNDTSELIYGEAFKDMPGQFYVSTKGMPTKLDTAEKAIAAVKRSIERLGVK
                     KIDFYHIWCIRKLEHYELAMKPGGQYEGLLQCQKEGLIDHIVLSSHQTGNEVSKILNDG
                     KIDGILLGVNILNFTYRWEGVQTAYDMGYGVVAMNPLAGGAIPTHEKELSFLAEGNGSA
                     TEAALRFVISCPQISVALNGFTNKEHIDMACRIADEAKPMSDQELERIRKMVSQSMNEA
                     CTGCGYCDKCPKGIPIPSFMQLYNEKHVFHKSDEQMKNILNDSFEWGILAGKRGLESEC
                     IECGLCERECTQHLPIIERLKEISMWAGNQDS"
ORIGIN
        1 ataggtaaag tctgtgtatc taaaaaatct cggtcaacaa tgtaatatgc acaaatatct
       61 gtagtttcag taggtccaaa aagattagca tacattaagt gggggaaatg ctttttccaa
      121 taattaagat atttcaatgg catgacctct ccagcaaaca gaactttttc aagatattca
      181 ggtttacaat atttgaataa atctaatttg gctgcaattc ctagcgctga tggtacccaa
      241 taaattgtat ttatccttcg cttattcata aattcaataa gcttgatagg gaatgtaaaa
      301 aactccttag gtatcatctg atatgcacta ccagtaaaaa atgaagcaaa catatcagta
      361 actgacatac tgaaataaag tggtgtctga gaaccaaata ctattttctc atctatctta
      421 aaacaagtaa taaaccaatc aatgtaactt ataacgttct gatgagtaag caacgcacct
      481 tttggacttc ctgttgaccc tgaagtaaat attgaatatg caggatcagt agaaagaatc
      541 tgagaacgga ctttatcaag taactcatcc ttcacttgtt ttttcatgat ttccccaaat
      601 tccatcttat gacattcaac ttgaaaatta tttactatgt catggaattc tgattcatat
      661 atgactgctg ctggtgtaag ggtatccatt atctttgcta gtctatcaaa tggtgaatca
      721 cagtcaagaa ctacataaaa atttccgctg tacagaaccc ctaacatagc agcagtaaca
      781 ttaatattgc gattcataag aattgcaaca ggttttttca tcaaatcctt ttctgcaatt
      841 gtagaaccta ttactctagc cttttcttgt agcagtttat aggttatctc agacttactg
      901 tctccaacag ctacattttc tggatataag cttactgttt tatcaaaaaa atcaagtatg
      961 gttttattca tctgactgct cctttataaa tatggtctaa tatgggaaaa gcatcattat
     1021 tatcactgca aactgatata acaagttttt caaaaatata actgttaata taataatcat
     1081 ttactttgtc attctcagaa aatattggtc tatcactgtg aatattatct atattttctc
     1141 ttggtgtcac atcaattgta ttaataggat aatctattcc tcttccaata catttaataa
     1201 aactttcttt tctagtccac agtaaggtaa atgctgtatt tctatctttt gatgattcaa
     1261 tatattcact ttctctatga gtaaaaaaat attttacaat attattatta aattcttgta
     1321 ccgcctcgca atcaatacca atattgtatg aacgctcagc ggctatcaca atatactccc
     1381 cagcatatga acgacttact gagatatctg gataatcctt caagtaaggt ttttcaccga
     1441 tacctccatt aaaatttgaa actggaatac caaagcttat ggcggtgaat tgggaagctg
     1501 cttgttccgt agctaaaaat cgttttattt ttttcttatc agtaaaaagt tctacacgtt
     1561 cttgcctctc ttttggcatt aaagatacat tgaatcgttc tgtattattt acatcaatat
     1621 ctttaattat tgctaatgct ttcacaacct cacctccaag ctatgaacat agtttatcat
     1681 cttaaaatta aattatctca aaataaacat taaaatttct taaatgttac attaagattt
     1741 cttaatgtta cgttaataaa agcaataaaa aaagattgct tcttaggcaa tcttgatcaa
     1801 aatatcttat ttacatactt ctatgatgta ttttccaata atctcactat ctacaatata
     1861 attggtatga gtatgatact taaagatttt taaaatacta ttttttacgt tttcatgaat
     1921 taaataaaga tgttttttag taataacgtc aaaacttcct ccagttatgt aagtaggtac
     1981 cttaattttt gaaaggtcag acaaagaaat attgggttct tgtaacatca tcttaacctt
     2041 ttgagattta ttaatgatat acattaactt catcatgagt cttgaacaaa ttctcagtcc
     2101 tttgggatta atgttaggac cactgacaat aagctttgaa aacaagtttg gatattctga
     2161 cgcaagcata agtcctacaa ttcctccatc actaaaacca tagtatacag gatttttgat
     2221 attaagctga ttaatgaatt catagatatc cttagcatgg tcttcataat gcagttcaga
     2281 caccttagag ctgttcccat gatctcgaaa gtcaatagca tacacagtaa aatattcttg
     2341 taaaagtgaa attgacctat taaaaatctc gtgtgtttca ccattcccat gaagcataat
     2401 caaaggattt cctgaaccat atttttgata aaataacgtg acttcattaa cttttattaa
     2461 catattctta ttcctcttct gtcagaggga aagttatttc atatactaca gatagcccct
     2521 ccaacttttc ttgtataagt tcaattgttc ctctatgagc agtaacaatt ttcttgacta
     2581 tagccattcc cagtccagaa cctttcagtt tgctacgtga ggaatcaccc attgaaaatg
     2641 gattaaatat agttttcttc aggtcatctg taattccaat accattgtcg ccgatacgaa
     2701 tgatacattt atcatctagt ctttctagaa caaaggatat gatagtaccc ttctcgttat
     2761 attttacagt atttgagata atattttcaa atacacgctt catctgaaat tcgtccagca
     2821 tacaataaat aacttcatct ggtatctgaa tatcaagctc aaaacctaag aattctagtt
     2881 cctcatactt atctatgaga taatttcttg cgaattcact gatgtttgtt tttacagtct
     2941 gaattttgaa atctggtctt tcaagcttac tatattcact gaatgtatta ataagctctg
     3001 ctagtgaatt ggactttttg tatatggtat tgagatattc actacgctca ttgtcgttta
     3061 cttttttatc agctacagct tttgcatatc cttgaataac agttataggt gtttttaagt
     3121 cgtgagaaat gtcagccagc atcttttgtt tttcctgttc taccccaata cgtttcttct
     3181 ctgtttcatg taatttcaaa gccatatcat tgaagctgtt acagatctct tcaaattctc
     3241 taggtccttt gtattttata atcttatcag tcttaccttc tgcaatgtgc tttattgcgt
     3301 tacctaaaag cagaagaggt tcttttactt ttttattgag actgattaca aaaaataata
     3361 ctgcgacaac aaaaaatact agatacgcaa tcccaatata attaataacc ttcatagagg
     3421 tattcaagtt atcggaatca acgttacggc aaaaaaccac attatatatt tttccatttt
     3481 tagaagtata acttaacttt gagatattaa tatcatcttt atattttcct gttaaaaggt
     3541 tgaagtcaga ttctgaaagt cgtttttcta tattggggat atctgagaat aatatattaa
     3601 aattactatc aagtattata tttgatttga tagtattatc ttcttcatct actgtttcat
     3661 atgcaacatt aatgtatttg ttatcatctt gacctacata ttcatctatt gatattgtaa
     3721 gtccagcttc actttcaggt atcaaatcca cttgcttctt agttaaatta aaagattcag
     3781 cgccttcact tctgtagatt acattgttct tatcatcaaa aacagtaaaa aaaccgtctt
     3841 ctccaagtaa tctttcccgc tgaaaagaat cataattacc ctcaataaaa aactctttac
     3901 tatcctcaaa atgggcagaa taataattag caaaatcgaa atatattaca gcaattccga
     3961 taatacctgc aagaataaga actgctaaaa cactcacagt aaatatgata aaattgcgga
     4021 ctattattcc aaagaaaccg agtctttttt tacttatctt caattttata tcccaaacct
     4081 ttcacagttt ttatatattg attaccattt ttattcatac cgattttttc acgcaagtga
     4141 gatatatgca ccgtaacgga attatcatcg gtttcaaagt attcaccttt tatatgttct
     4201 attatctgta cttttgaaaa gattcgttca ggtaccctca tgagtaaggc aagaattttg
     4261 aattctgtag ctgtcaattg aatttcttgt tcctgcttaa ccaatcgaca gttatttgtg
     4321 tcaaggttta actctcctag attcaatata ccactgttct tttcgttgcc acttaatttg
     4381 taatgccttc ttaacgcagc atttaccctt gcaactattt caaggggatt gaatggcttg
     4441 gttatgtaat catcagcacc catgttaagt cctagaattt tatcaatatc ctcattcttg
     4501 gcagataaga ttaaaacagg tatattactg attttcctta tttcacgggt aagctcatag
     4561 ccgtccattt ttggcatcat gatatcaaga attgcaaggt caatcttatt ttcttttact
     4621 aatgaaagac cgtcaagccc gttatctgca attaatacct cgtaaccttc aacttcaaga
     4681 tataatttta ttacttttga tatttcacag ttgtcttcag caacaagaat cgtgttatgc
     4741 atattgttcc cctttacgct tttctaatgt tttcttatca atattgtaat taattatagc
     4801 atataatatg cactgtgata atattatttt tttgtaagtc gaacccagtc agaataaatg
     4861 tttttttctg actcaatgta taattatctt aattattata taattataaa taatttattg
     4921 catttgtttg caaaatatgg tatatttatt aaaaaggtat tacattctgt ataatcaaaa
     4981 taatgaatgg aggattacag atgagtttag aacacaaaaa acattttaaa gaagtatctc
     5041 cgactgagac ggttactaaa ctaaaaaaca tcttaagtga acttggtatt gaaacagagg
     5101 aaacttggtc agaggaaagt tttgtggaaa cctactcatt acgattgaca ttcaaaggaa
     5161 ctaccattgg aactaatgga aaagggataa ctcgtgaata tgcattagca agtgcatacg
     5221 gtgagttatt tgaaaggtat caaaataatc tattaggtat tattatcagt aaaaaggaaa
     5281 gtggttctga tttctacgaa gctccagatg aaaaaattgt gacagctgaa gaaatcgtta
     5341 gctatgacaa ttcactcatg aatttgtatt tttcaaatcg ttcaaaaaaa gatacttcta
     5401 ttaaagaaaa agcaaaagca tttaaaagca cacaaaagat ggacttgctt aagcataatc
     5461 ttgaaaatcg ttatattgtt gtacccttct atagtatgcg tgatgaaaaa atagtctatc
     5521 tgccaagagg tacatgcaga cgtttttatg gaagtaatgg aatgtgtgct ggaaacactc
     5581 ctgcagaagc acttgtacaa ggcttgtcag aaattattga aagggttgtt caaaaaaaat
     5641 tatttttaga aaaacctact ctaccagatg ttccagatga ctatataaaa agatacccat
     5701 atattgataa aatgtatgca aagctaaaag aacaagaaga ttactatgta accttaaaag
     5761 actgctcttt tggcggaaag tatcccgtag cagctttagt cattgttgag aaaaacactg
     5821 gaaattatgg tgtaaaacta ggatgtcatc ctgattttgg agttgccatg gaaagagctt
     5881 ttacagaagc aactcaagga aatgatattt ttacatatgc acaaagaagc catattgatt
     5941 ttagcaacct ggattctagt aattcaatga acatttataa tagttataaa ctaggtgtgg
     6001 gacagtatcc ttatcaacta ttcggcaaca aaccaaccta tgaatttgtc ccattaaaag
     6061 atgtttcgtc aatgagtaat caggaaatat tgaattattg ggtaactgat ataattaacg
     6121 atggatacga tgtattaata agagatgttt ctaatctagg atttccatca tattatatcg
     6181 tgattcctgg attatctgaa atgtgggaag ataaagacgt aattttcaga gcttataata
     6241 ctagattctt catttcaccg ttagttgcga atcccactaa aatcaatcgc tccaatacaa
     6301 aatatattat tgcaactatg gaattcttta tgaattgtgt attagagaat actatggaat
     6361 cttattgtaa attcactgaa gattggcctt gtgagaactt tggttgtgat accagatttt
     6421 ttgtagtaat gtgtcatgta ataaatgaag aattcagtat tgctgctgaa aaaatgaaat
     6481 atattgttaa tatggcacag cgagcatcca ttcctaaaga tcaattcaag ttttataaag
     6541 gtatgtataa ttattttgtt gcaatgtctc agatacatga ccataataaa gtactagaat
     6601 atcttagggt attctatagt gaagatacat gcaaaaaact agacaatcta ttctgtgtgc
     6661 ctaaacaagt aatggttaaa ttatttgtac caaaatcaac tgattctcct aagcttcaag
     6721 aagtaaagga ttcttataaa caatgtcaag aaattattca taaattgcat tcggcccaaa
     6781 taaaaaatcc tataaaccaa aaagatatct atgaactatt tgcataaata tagaaacata
     6841 tttgttttgt actcttattc acgttattta gctgaattgt aattatctag tagtttttaa
     6901 tatgctagtc attgttacaa tcagttagat aaatataaca actaatgaaa ggaggcatta
     6961 tttatgaaag aacaagaaaa atatggtttg tttttggata atgaattatc tagtttaaca
     7021 tcatcttttg atattagatc agaagataca gatgatcttc caattgctgc cgctgctgca
     7081 ggaccagaag gtgccggagc tgctgctgct gctccagaag ctttttatgc tcctggtgga
     7141 caagcgttag cttaagttaa caattagtcc tattggaaat ttctaattaa cgacttatta
     7201 gttacatata aaataaagta tccataaata aaacagtatg tattgcttat cagaacgata
     7261 cctgtattac aacacttctg atttgtagta atacatactg cttttactat atataatcta
     7321 taaatataac tttcttacag caaaataaag aacgaatagg tggtgtatat atgcaaagtc
     7381 catgtataaa tcagaatctg gaactaatgc caaaagatga tggcagaata tttattttta
     7441 acaaatctac aaaaaagacc tattccctag gtcgtaatga atataaagta ttaagtatat
     7501 tagatggtaa taaaacaatg gaagaaattc ataaaaagtg taataactat tcattgtatg
     7561 atatagaaaa attaataaat gagttttcaa aaataggatt attaaagaca gaggacacca
     7621 atcaatcccc taccgatatt attgaaaaaa aagttgtaga tattaaagac tatcaaaaaa
     7681 aacgattttc cataatggat aaaaaaatat tcaagatttt ttcattgaaa aaaggtattc
     7741 ttcatcctga taatagttta gataaagata aaatgatctt cagaattctt tatttttcta
     7801 ttatctattt gtcattacct atatttttga ttggattact tgtttcaata ggtacttctt
     7861 cattaagtaa ttccttgaca gctccctctt ggtatatatt cattcccgca tttttgataa
     7921 gtattttctt tcatgaattt gcacacatca ttgtggcaaa aaacaatgga gctcatatag
     7981 cagaagttgg aatacgttta atactaattg ttccttgtat ctactcaagt attgtaggta
     8041 tctcttatat tgaaagtaaa attaagagaa tattaatctc agctgctgga attttactta
     8101 atttacaagt agctggaatt gggtttattc ttactgcttt cacagaagga aatgcaaata
     8161 attttttcct atggctatct atgtttaatc tcatgttggt aatgtttaat ctaatatttt
     8221 tattaaaatt cgatggttat tacatactaa cggaattatt aaacgataaa aaattaaaag
     8281 agaactcgtt gtcctttatt agttatatag tgtttggaaa gctttttaga aaagcaacag
     8341 taactaatat gagaataaag ttatctctta agaaaaagat aatatatgtt ctttacggaa
     8401 ctttaaatat agcttttgtt ggatgtattt tcgtaacatt cattacaaat atcataagat
     8461 ttatatccag taaaatataa tttgataatg cttttaatct aaatctatat accttataaa
     8521 aaaatagaat atgattaatt ttctatagaa caacagaaat aatatttttt tctgaataat
     8581 attaatatta taaattaata ttgctccaaa actaaatatt catattattt tcacattata
     8641 tattgttcta tatttatctt agtgttatta taagtaaggt atttcgaaat acaaccattt
     8701 ttatgaggag gaatttagat tatgagtaag gtttttaatg tacaaaaatt atttgtacta
     8761 acagctgttt ttttaatgtt aggggcatct actgtatttg cttcatcaga ctattatctt
     8821 gacactccaa cagaaattga ttttaattat attgaagaat cagtaaaaga tgctaataag
     8881 gtaatggata tggaatttac tgtagtagat gatgataatg tgcaagatgt aataaaacaa
     8941 aatatagaca ttttaaaatt caacccattc aaacttagct tcagagatgg gcattattta
     9001 tatatagctg tcggatacgg cgaacaaaga acaggaggat atagtattca agtaaatgaa
     9061 ttatacgaat cccaagatcg tattgtaatc tcaactgagt tattatgccc tggtgcagat
     9121 gacattgtta ctatgaatat tacatatcca tatttaatca taagaacaag tgatttgttc
     9181 ttgccaattt attataagta aagacattta tttattaact tatttaagac aacagtttaa
     9241 ataccctata tattgatggg ttttaaactg ttgtcctttt atattacttg cttctcttca
     9301 ctacttttgt tatcatatac ttttttattc tattttttta caactggtat ccaaacttct
     9361 tcaataaact ttccatcacc tttattataa tacatttcaa attcaggtcc atctgcatgc
     9421 tcatatccag aattaggaaa ccattctggg aagatacgtt tccatatgtt ttgtattgct
     9481 tctgttccgc cttcctgtgt acattctgca gttgagaata tagcccatgt tgtagcagga
     9541 acagaaagct tggtataacc agcaggtaac gaaacatttt ccggaggata atagcaaatc
     9601 atataagata atataccttc tctgaaattg tataatgctg catgaagaca ttcatcttga
     9661 tccagattaa aatctttatg catatcatca agttttccat tttcaatgca tttatgccaa
     9721 aatgctggaa tatctatata ttgacgatta tcaacagcat ttatttcttt tgatattcca
     9781 tacatctcaa atgcttcttt tctttcaatt ctataatcca tctccacatc tcctttaata
     9841 gaaatatgaa aggtcatccg tggataagct ttgagtgctg taccctgttt gcgcgctaac
     9901 ataggcatga caccatgtag attcttgaaa gctcgtgaaa aagcttctgt tgattgataa
     9961 ccatatttca tagctacatc tacaactttt atatcagtat tttgcaattc aaaagctgcc
    10021 attgtaagtc tcctacgtct gatatatgca gctaaagtaa ttcctgcaat aaatggaaac
    10081 atacgttgaa aatgatattc agaacaacaa gctcttctgg ctacctgttc ataactgata
    10141 ttttcagcaa gatgttcttc aatatagttc attgcattat tcatactatc aagcaaatcc
    10201 acgtaatccc ctccttcgag atacagtata tcaagttttc tttatggaag cattgcattt
    10261 tatgtgcatt aaatatcaac aaaccaatta tattataaag cttaactgtc ctgattacca
    10321 gcccacatac ttatttcttt taatcgttca atgataggta gatgttgtgt acattctctt
    10381 tcacatagtc cacattctat acattcactt tccaaacctc ttttaccagc tagaataccc
    10441 cattcaaaag agtcattcaa gatatttttc atctgttcat cagatttatg gaaaacatgc
    10501 ttctcattat ataattgcat aaaacttggt ataggtatac cttttggaca tttatcacaa
    10561 tagccacaac cagtacaggc ttcattcatt gattgactta ccatttttct gattctttct
    10621 aattcttgat ctgacattgg ttttgcctca tcagcaatcc tgcatgccat atctatatgc
    10681 tctttgttcg taaatccatt taacgcaact gaaatctggg gacatgatat tacgaatctc
    10741 aatgccgctt cagttgctga gccatttcct tctgctaaga aactaagctc cttctcatga
    10801 gtgggtatgg ctccaccagc caatggattc atagctacaa caccataccc catatcatat
    10861 gcagtctgga caccttccca acgataagtg aagttaagta tattcactcc taataatatt
    10921 ccatctatct tcccatcatt caagatttta cttacttcgt tccctgtttg atgagaagat
    10981 aacacaatgt gatcaatgag accttctttt tgacattgaa gaagtccttc atattgtcca
    11041 cctggcttca ttgcaagctc ataatgttca agttttctaa tacaccaaat atgataaaaa
    11101 tctatttttt taacacctaa tcgttcaatt gatcgtttta ccgccgcaat tgctttttct
    11161 gctgtatcta attttgtagg catccctttt gttgaaacat agaattgccc tggcatatct
    11221 ttaaaagcct caccatatat taattcactg gtatcattgc aatagcctgg tgctgtatca
    11281 aaatagttga ttcctttact gttagcataa cgaaccaact ctgcattttc ttctagtgac
    11341 cttgattcgt caaatctcat acctccaaaa ccaactgctg acactttttt cccagtattt
    11401 ccatattctt tatagatcat aatatataac tccttccata gccaacttaa aaatgtcctc
    11461 atgattacaa gagttagtat aactgctaga gttaactcta agtcaaggtt tttataagat
    11521 aagctatgat tttttattaa tactattatt ctctataaag tattatataa ttaattactt
    11581 tgatactgtg tttttaccat agatgcaaat attccttgtt gctcttctaa ctgtgaaggt
    11641 gtacctgttt ccactataga accgtctttg ataaccacga ttttatcagc tcctgaaaca
    11701 gttctcatac ggtgagcaat aattagaact gttttgtttt ttataagttc acttaaggca
    11761 ttttgaattt tactctcatt ctcagtatca agggatgctg tagcctcatc aagcaagatg
    11821 ataggag
//
