LOCUS       NZ_CP047645            20747 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Bacillus subtilis strain PMB102 chromosome.
ACCESSION   NZ_CP047645
VERSION     NZ_CP047645.1
KEYWORDS    RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4103088)
  AUTHORS   Wu,J.-J., Huang,J.-W. and Deng,W.-L.
  TITLE     Bacillus subtilis PMB102 suppresses Alternaria leaf spot on Chinese
            cabbage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4103088)
  AUTHORS   Wu,J.-J., Huang,J.-W. and Deng,W.-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) PhD program in microbial genomics, National
            Chung Hsing University and Academia Sinica, No. 145, Xingda Rd.,
            Taichung 40227, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-JAN-2019
            Assembly Method                   :: Canu v. 1.8; Pilon v. 1.23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.97x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina GAIIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018141065.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:52:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,262
            CDSs (total)                      :: 4,141
            Genes (coding)                    :: 3,965
            CDSs (with protein)               :: 3,965
            Genes (RNA)                       :: 121
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 121 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 21 of 176
            Pseudo Genes (multiple problems)  :: 27 of 176
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:29:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3479233
            Orig. end                         :: 3499980
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047645.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Ph.D. Program in Microbial
            Genomics, National Chung Hsing University and Academia Sinica, No.
            145, Xingda Rd., Taichung 40227, Taiwan.
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="9"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10747)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(437..1369)
                     /gene="hprK"
                     /locus_tag="GSN02_RS18000"
                     /old_locus_tag="GSN02_18005"
     CDS             complement(437..1369)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="GSN02_RS18000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18005"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003228097.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
     misc_feature    1481..1496
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            1551..2741
                     /gene="nagA"
                     /locus_tag="GSN02_RS18005"
                     /old_locus_tag="GSN02_18010"
     CDS             1551..2741
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="GSN02_RS18005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18010"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_046160894.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.4e-42, bitscore:
                     142.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSTLDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKEESIIASMGHTDADSAMLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     gene            2738..3466
                     /gene="nagB"
                     /locus_tag="GSN02_RS18010"
                     /old_locus_tag="GSN02_18015"
     CDS             2738..3466
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="GSN02_RS18010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18015"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_014478107.1"
                     /transl_table=11
                     /translation="MKVMECQTYEELSQKAARITADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYARLSSNDPNSYHFYMNDRLFQHIDSKPSRHFIPNG
                     NADDLEAECRRYEQLVESLGGTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIRTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            3484..4215
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="GSN02_RS18015"
                     /old_locus_tag="GSN02_18020"
     CDS             3484..4215
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="GSN02_RS18015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18020"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_014478108.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(4234..8103)
                     /gene="yvnB"
                     /locus_tag="GSN02_RS18020"
                     /old_locus_tag="GSN02_18025"
     CDS             complement(4234..8103)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="GSN02_RS18020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18025"
                     /product="metallophosphoesterase"
                     /protein_id="WP_212082498.1"
                     /transl_table=11
                     /translation="MRKYTVIASILLSFLSVLSGGHHESKAFPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRNNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKRLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADSYEFIEIFNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTEGRADRIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKAVIERTFKTEREHPDQPEVIDSDADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPERETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDQDIAWMDQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            8268..8729
                     /gene="pchA"
                     /locus_tag="GSN02_RS18025"
                     /old_locus_tag="GSN02_18030"
     CDS             8268..8729
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pchA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="GSN02_RS18025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18030"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_021480697.1"
                     /transl_table=11
                     /translation="MNNSIHDELFQAIQQFALKRDKRVWQKVQIPSSQHLDHLKKDWTL
                     TQLHIVSCLHTSKNVNNSFLASRLHISKAAVSKAIQALLKHNIITVTKKPGNKKEIFYT
                     LTESGKELAGLHERLHQKAKEQYKQLFNEFSIDELKTVTAFINLWIKHM"
     gene            complement(8768..9985)
                     /gene="cypX"
                     /locus_tag="GSN02_RS18030"
                     /old_locus_tag="GSN02_18035"
     CDS             complement(8768..9985)
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="GSN02_RS18030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18035"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_038829907.1"
                     /sec_met_domain="p450 (E-value: 3e-36, bitscore: 123.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKIDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEDFCYAEFGLYTRGPVSLH
                     IVFDRA"
     gene            complement(10001..10747)
                     /gene="pchC"
                     /locus_tag="GSN02_RS18035"
                     /old_locus_tag="GSN02_18040"
     CDS             complement(10001..10747)
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="GSN02_RS18035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18040"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_021480699.1"
                     /sec_met_domain="CDPS (E-value: 1.9e-83, bitscore: 277.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEPLTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFMDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITCEG"
     misc_feature    10873..10888
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     misc_feature    10875..10889
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(10876..10891)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     gene            11172..11681
                     /gene="pchR"
                     /locus_tag="GSN02_RS18040"
                     /old_locus_tag="GSN02_18045"
     CDS             11172..11681
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="GSN02_RS18040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18045"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_003228073.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            11702..12913
                     /gene="pchE"
                     /locus_tag="GSN02_RS18045"
                     /old_locus_tag="GSN02_18050"
     CDS             11702..12913
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="GSN02_RS18045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18050"
                     /product="MFS transporter"
                     /protein_id="WP_003228068.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(12941..13300)
                     /gene="yvlD"
                     /locus_tag="GSN02_RS18050"
                     /old_locus_tag="GSN02_18055"
     CDS             complement(12941..13300)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="GSN02_RS18050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18055"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(13302..13499)
                     /gene="yvlC"
                     /locus_tag="GSN02_RS18055"
                     /old_locus_tag="GSN02_18060"
     CDS             complement(13302..13499)
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="GSN02_RS18055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18060"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            complement(13504..14601)
                     /gene="yvlB"
                     /locus_tag="GSN02_RS18060"
                     /old_locus_tag="GSN02_18065"
     CDS             complement(13504..14601)
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="GSN02_RS18060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18065"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_070547846.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDIQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            complement(14626..14952)
                     /gene="yvlA"
                     /locus_tag="GSN02_RS18065"
                     /old_locus_tag="GSN02_18070"
     CDS             complement(14626..14952)
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="GSN02_RS18065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18070"
                     /product="protein YvlA"
                     /protein_id="WP_032726939.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LREA"
     gene            15170..15400
                     /locus_tag="GSN02_RS18070"
                     /old_locus_tag="GSN02_18075"
     CDS             15170..15400
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="GSN02_RS18070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18075"
                     /product="hypothetical protein"
                     /protein_id="WP_003235419.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIHEQKRMNREMIAELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    complement(15186..15201)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            complement(15599..16420)
                     /gene="hag"
                     /locus_tag="GSN02_RS18075"
                     /old_locus_tag="GSN02_18080"
     CDS             complement(15599..16420)
                     /codon_start=1
                     /gene="hag"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391416.1"
                     /locus_tag="GSN02_RS18075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18080"
                     /product="flagellin Hag"
                     /protein_id="WP_029318328.1"
                     /transl_table=11
                     /translation="MRINHNIAALNTLNRLSSNNSASSKNMEKLSSGLRINRAGDDAAG
                     LAISEKMRGQIRGLEMASKNSQDGISLIQTAEGALTETHAILQRMRELTVQAGNTGTQQ
                     TEDLTAIKDEMDALTEEIDGISQRTEFNGKKLLDGSETAGFTFQIGANAGQNINVTIGN
                     MDATTLGVKGIDVSADDFSFDTEIVKIDSAITSVSNQRAKLGAVQNRLEHTINNLGASS
                     ENLTAAESRIRDVDMAKEMSDFTKNNILSQASQAMLAQANQQPQNVLQLLR"
     gene            complement(16758..19631)
                     /gene="uvrA"
                     /locus_tag="GSN02_RS18080"
                     /old_locus_tag="GSN02_18085"
     CDS             complement(16758..19631)
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="GSN02_RS18080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GSN02_18085"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_003228057.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVAPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALAFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
ORIGIN
        1 gaagttcccc cagcggccga tcgcctggcc gagcaaaatg ctgggcgcgg caatgtccgc
       61 aagcttccag aacgaaagtt ttttcactct cgaaaacaca taacccgtta aaatggcgcc
      121 gattaatccg ccatgaatgg caatgccgcc cttccaaatc ttaatgattt ctcccggatg
      181 tgccgcataa tagtcccatt caaaggcaac gtaataaatt cgcgcgcaaa taatcgcaat
      241 cggaatggca aacaggacaa gatcaataaa cgtatctttc tgcagccccc gtttttcact
      301 ttccctcatc gcaatccaca gcccgagcaa agcgccgaga ccgataatga ttccgtacca
      361 atggacggct aacggcccca gctgaaatgc tattggattg agtggttcta tcgcttcatt
      421 catttaacgc caactcctat tcttcttgtt caccgtcttc aatgacgtcc gccagcttgt
      481 tggtgaattg ttcagcggca ttcagcccca tccgcttcaa tctgaagttc attgctgcca
      541 cttcgatgat gactgcaaga tttcggcctg gacggacggg aatcgtcagt tttgtgattt
      601 ctgtatcaat gattttcatg gtctcttcct caagtccgag ccggtcgtat tgcttgccct
      661 gctcccaaag ttcaagattc atgacaatcg tgattcgttt attgcttctg accgcgccag
      721 cgccaaatag cgtcatcacg ttgataatgc caagccccct gatttcaagg agatgctcaa
      781 ttaattccgg agcgtttccg acaagagtat cctgatcctc ctgccgaatt tcaacgcaat
      841 catcggcgac aaggcgatgc cctcttttca caagctccag cgctgtttcg ctttttccga
      901 cgccgctctt tcctgtgatc agcacgccga caccatatat atcgacaaga acgccatgaa
      961 ttgctgtggt aggcgccagc ctgctctcaa ggaagttggt taaacggctt gacagtcttg
     1021 tcgttttcag cggcgatctg aggacaggca ccccattttt ctcggaggcg tcaatcagct
     1081 cctgcgggat gggcatatct ctagaaagaa taatagctgg tgttacatca gtgcacagag
     1141 aatccattcg ctgttttttc tcctcttcag gaagctgttc aaagaaagaa agctctgttt
     1201 ttccgagaag ctgcacgcgc tctctcgggt aatatgtaaa atatccggca atttcaatgc
     1261 ctggtcttga taggtcactc attgtaatcg ggcggttaat tccttcttct ccgctgatta
     1321 attccaaatt gaactgttcc attacgtctt ttgtgcgaac ctttgccacg atatgttcct
     1381 cctgttccgg gctgccccga gcttgctcac aatactttca ttttatcact ttcaggcttg
     1441 aacctaaaac agattttata aaaggcggga aaacacctca gctggtctag atcactagtc
     1501 tgaaaaagag taaaataaag gtattcaaat tccagaaagg cggatcatct atggcagaga
     1561 gtcttcttat caaagacatt gcgatcgtga cagaaaatga agtaatcaaa aacggctatg
     1621 tcggaatcaa tgacggaaaa atcagcacag tctcaacaga acggcccaag gagtcatatt
     1681 caaaagagat tcaagcacct gcggattccg ttttgcttcc cggcatgatt gacattcata
     1741 tccacggcgg ctatggcgca gatacaatgg atgctagctt ttccacgctc gacatcatgt
     1801 cgtcaagact gcccgaagaa ggcacgacga gctttttagc cacaacgatt acccaagagc
     1861 atgggaacat ttctcaagct ttggtgaacg caagagagtg gaaagcggct gaagagtcct
     1921 ctttattagg tgcagaactg ctcggcattc atttagaggg cccctttgtt tcgcctaaaa
     1981 gagccggagc gcagccaaag gagtggatta ggccttcgga tgttgcgctt ttcaaaaagt
     2041 ggcagcagga ggcgggcgga ctgatcaaaa ttgttacact tgcgccggag gaggatcagc
     2101 attttgaact gatccgccat ttgaaagaag aatcgattat cgcgtccatg gggcacacgg
     2161 atgccgattc cgcaatgctg tcggatgccg caaaagcagg ggcgagccat atgacccatc
     2221 tgtacaacgc gatgagcccg tttcatcacc gagaacccgg agtgatcgga acggcgctgg
     2281 cccatgatgg gtttgtgaca gagctaatag ccgatggcat ccactcccat cctttagcgg
     2341 ctaagcttgc ttttttggcg aaaggcagca gcaagctcat tttaattacc gattccatga
     2401 gggcaaaagg actgaaagac ggcgtttatg agttcggcgg ccaaagtgtg acggtaagag
     2461 gacgaacagc tcttctttca gacggaaccc ttgccggttc gatcctcaaa atgaacgaag
     2521 gtgcacggca tatgcgtgag tttacaaatt gctcttggac ggatatagct aatataactt
     2581 ctgcaaacgc ggccaaacag ctgggcatct ttgaccgaaa aggcagtgtg actgtgggaa
     2641 aagatgcaga tttagtgatt gtcagcagtg attgcgaggt gattctcacc atttgccgcg
     2701 gaaacattgc atttatatcc aaggaggctg accagatatg aaagtaatgg aatgtcaaac
     2761 gtatgaagag ctaagccaaa aagcagccag aataacggca gatacaatca aagaaaaacc
     2821 tgatgccgtt ctcgggttag cgacgggcgg cacaccggaa ggcacttatc gcgagctgat
     2881 ccgcctgcac caaactgaga atctctcatt tcaaaacgtc actacagtta atttggatga
     2941 gtacgccaga ctttcaagca atgacccgaa cagctatcac ttctatatga atgaccgttt
     3001 atttcagcat atcgacagca agcctagccg gcattttatt ccgaatggaa atgcagacga
     3061 tttggaagcc gaatgcagac gctatgaaca gctagtcgag tccctcggcg gcactgacat
     3121 tcagcttctc ggcatcggcc gaaacggaca catcggcttt aacgaaccgg gaacctcttt
     3181 caagtcgcga actcatgttg tgacattaaa tgagcaaacc cgccaagcga atgccagata
     3241 ttttccttcc atagacagcg tgccgaaaaa agcactgaca atgggaattc gaacgattct
     3301 ctcaagcaaa cgcattctgc tgcttatttc tgggaaaagc aaagcagaag ctgttcgtaa
     3361 gctgttagaa ggaaacataa gcgaagattt tcccgcatct gctttgcacc tgcattccga
     3421 tgttacggtt ttgattgatc gtgaagccgc gtcattaaga ccttgaaagg aacatgctga
     3481 cttatgaata tcaataaaca atcgcctatt ccgatttatt atcagattat ggagcaatta
     3541 aaaacccaaa ttaagagcgg agagctgcag ccggatatgc ctcttccttc cgagcgcgaa
     3601 tatgccgaac aattcgggat cagccggatg acagttcgcc aggcgctttc taatttagtt
     3661 aatgaaggct tgctctatcg cctgaaaggg cggggcacct ttgtcagcaa gccaaaaatg
     3721 gaacaagcac ttcaagggct gacaagcttt actgaggata tgaaaagccg cgggatgaca
     3781 ccgggcagca ggctcattga ttatcagctt attgattcaa ctgaggagct cgcggctata
     3841 ttaggctgcg ggcacccctc ctctatccat aaaatcactc gggtgcggct ggcaaatgat
     3901 attccgatgg cgattgagtc ctcacatatt ccctttgagc ttgcgggtga attgaacgaa
     3961 tcgcattttc agtcgtcgat ctatgatcat attgaaaggt ataacagcat accgatttcc
     4021 cgtgcaaaac aggagcttga gccaagcgct gccaccacgg aagaagcgaa tattcttggt
     4081 attcaaaagg gagcgcctgt cctattaatt aaacgaacga catatctgca gaacggaact
     4141 gcttttgagc atgcaaaatc cgtatacaga ggcgaccgtt atacatttgt ccactatatg
     4201 gatcgtcttt cataaaaaag cctccaaccc tttttaagga ttggagacat ggcgaaaatc
     4261 aaactggtct ggtgccggac gatatgtttc ttttttcgtc ttgaacttcc agatcggtga
     4321 tttcgttttg ccgttaaaac tgtcttccac tataatgtac caataataaa cagactgcgg
     4381 ttcaagatga tcccagcgga attcagctgt atccccgctc ttcacttgct cccgttttcc
     4441 gagctcttca ttggtatata cgttacattc aaaataatcg gtttccacct ttttgattct
     4501 tggcttcaaa tcaagcgaaa gggaaaattc atccttgttg ccataggtat ccgtatcata
     4561 gtaattctta tcctttacat acggagaata cgtcgatacg tgcaccatgt catttgcctg
     4621 gtcaaattga agcaaacgca aatacccttg accgccttct ggtccgccct gataatcggc
     4681 cagcatttga tgcacaagac gatccggctt cccatcacca tcatcatcca gctcatctgt
     4741 ttttctcatc gcactatgat aatgcccgct caacaccatc acgacattcg ggttcggctt
     4801 tacgatttct ttgaatattt tctctccgat cggacttctg tttccgctga ccagcaaata
     4861 ctcatgaaaa gctaaaatgg ccatccggtc aggatgcttc ttcagcacct gatccatcca
     4921 cgcaatgtcc tgatccgtta ttccccatcc catgtaaagc atgatataat cattgccgtt
     4981 gctagaaatc aggtcataat gtccgcggtt atttttatag gaaccgccat aatgcaattt
     5041 tttatcaaaa cgatcgctgc caaaatattt gccaaacgcc ctgtaagagc cgtccttatg
     5101 ccccacatcg tggttgccgg ccagcacgcc gtaaggtatt ccgctcttat ccagaacgga
     5161 cattgatcga tcagcgtttt tccactgtct gatatcggcg gaatcatcta caatatcacc
     5221 cgtgtggaac acatatttga tattgagctg cttttgatta tcctttatcc attcggtctg
     5281 tttatcaaaa atatgcggat agctttcggc gtaatactgt gtatcagaca tccagacaaa
     5341 tgtgtacata tccttcgcag ggggaatttc atcctgtaca atcacattca ttttcgactc
     5401 ccgtacaaaa tcggcggcaa tcacagacgc tttcagcgta aacggtttgt catccttcgc
     5461 gacgaagctg tcaacaggct gccattcgtt tgtccgataa ttccaagcat acatcgtcac
     5521 ctttctcccc ggcaaagaac tgcccttcca aaccgcttct gccagatcat tctcatcaat
     5581 tgatggatca accgtcactt caaagcgatg ataagggaaa agctctttgt tttctgtcga
     5641 tactgttttt ccatcagcag ctgatacccg ctccaattct ttattcgtaa atggggtctc
     5701 cctttccggc acaaagcttt tcggcggttc agtcagtgac gcgtgcttaa atattttcac
     5761 atgattgcgc gctgtatatt gaaatcccct ataaaaacca acatccatgg catcattcgt
     5821 cggatctttc acccggacag aaagcttggc cttatgcgtg tcggcatcgc tgtctataac
     5881 ctccggctgg tccggatgct ctcgttcggt tttaaacgtc ctctcaatca ctgctttatt
     5941 gcccgcgaga tccgttgctg tcacttttag agaatgtttt cccggcaaaa ggtcagaaga
     6001 tgacgtatga tatgggagtg taatgctttc tccatctaaa gacgcttccg cttcctcaat
     6061 tttgctccat ttatcataca tatccgcctg caggataagg tttcctttat acgtttgccc
     6121 ctcggtaata ttcggctcaa tgtgaggtcc tgaattatct acccgtacaa ccaggctgac
     6181 attttccttg ccgtcgcttg cttgaatatg gtgagcgcct tcagaaagct ttgatgtgtc
     6241 ccactccagc agctgtgatg taaacagtcc gtcaggcaga ttgaactgaa attgccagct
     6301 tttattcgag cgctgattat cgccaataaa gagttccttc tcaggagaca ccctctgatc
     6361 cctgatcacc gttccatccg ataacaccaa tcgggcgttt ttgactaaga agtcatcacg
     6421 gttttcagcc gtatcaaacg gagacacctt cgaacccgat cgtattgtaa tacgctgcag
     6481 ctgcttccct ttccgaagca gtgtctctgg caatggaaca gaataggtgc ggtatttatt
     6541 tgttgtatca tcgaacacct tcagcacttt tccttccatc gttaccgcgt ttttaaaata
     6601 aaggttcgtt ttcctcgtat cgaatgcaaa atatacttct ttttccatcg cgggctctgt
     6661 cacttgtttt ttcccgtcaa tccatagctt aatgcggtca gctcgccctt ctgtcgtggc
     6721 tttgaggaat tgagttcccg acagcgtgtc tctgttttca atattcatcc gcagaccgtg
     6781 cgacttgctt gtttgtttaa tatcaatcat tttgaccggc gttctcgctt cattgatccc
     6841 gtccccggcc gccacataat actctagctg atccttcccg atcaattcag gcgaatacac
     6901 aatataatgg tagagattat cgttgcggtc tttttcagca agaattcgct tgaacggttt
     6961 gttctcatcc gtccggtagt aaaaggccgc tgttttcact agacttcggt cccgtatgtc
     7021 agcccgcagc tcaatgcttt cagcagggcg gacaggcttg cggtcagtta aatcctcaat
     7081 gaccggcttt tctttatcga gcttgatttt ccgtttctgc tcgggcacct gtgcctgtaa
     7141 tacagttccg ggagacggat tcttttcccc gattgaaatg ttcagaagtt ctgcggttcc
     7201 gttcaacgga tacttataaa gcactgatga cagccggcgc agcgcatgtt tccgataatg
     7261 cgcaaccgaa atgtcctttc ctgtattcgt tgatatcaca acggctcgcg gcctcgtatt
     7321 ggccataccc tctgtctcgt ctatcaccgc gagatttttt ccttctttta gttgtgtctg
     7381 ataataacga ttgaaatcct ctgacgtcaa ttctggatgc ccggcaggct tcagccagac
     7441 gacaaacgtt tcgccggatg gtatcaagat cctttcatca ggtctccaaa tcagatcgga
     7501 gtcaggccct tcttttgggt agcggtaacg gatatggtag tctttaaaat caatcgttct
     7561 gtcagtcgta ttaaaaatct caataaattc ataactgtct gcacgtccta tattcttcgt
     7621 atcaaccgcc agctctgtga tcagaagctc cggaatatgg gcaaggtcag cctggtatcc
     7681 ttcaagcttc agtctcttcg tctccgactc cacccgctgc ttcccattcg acatcacaat
     7741 gtaatactca agctcgctgt tccaaatcaa acgtttcgga atttgtgcca aaaacttgcg
     7801 gaccgctccg ggggtcgggt ccatcggcac cattttgtag cccagctcat tactttgttt
     7861 ataaaaaaga aaggcggaaa atgcttgata cgaattatcg cgaacttgag cctcaatcat
     7921 tagattgttt ctcatttcag ctttcacatc aatttgagga ggattttcaa gcaagggtat
     7981 gtaatgaggc gtttgaaata gttcctgctg tacaacagga aaagctttcg attcatgatg
     8041 gccgccagac aaaacggaaa gaaaagacag taatatagaa gcaataaccg tgtatttcct
     8101 catcaaagca cctccctttt catgtatttt gcgtctgaac agggaagctt atgagttcat
     8161 ttttgtgcat aaagaacaaa aaaacgtctg cctcttgaaa attccactat tccccagtaa
     8221 actaaggata gttaaccagt taacaattaa atcagagagg caaattgatg aacaacagca
     8281 ttcatgatga attatttcaa gctatacaac aattcgcatt aaaaagagat aaacgcgtct
     8341 ggcaaaaggt gcagattccg tcctcacaac atctcgacca cctcaaaaaa gactggacat
     8401 tgacccagct tcacattgtt tcctgtttac ataccagcaa aaatgtgaat aacagcttct
     8461 tagcatcccg cttacatatc tcaaaagcag ctgtttcaaa agcaattcaa gctttgttga
     8521 aacataacat cattacagtg acaaaaaagc cgggcaacaa aaaagaaatt ttttatacgt
     8581 taactgaaag cggcaaagaa ctggcggggt tacatgaacg gcttcatcaa aaagcaaagg
     8641 agcaatacaa acagctcttc aatgaatttt caattgatga gttgaaaaca gtgactgcgt
     8701 ttattaactt gtggatcaag catatgtaaa aaagcctcac ccgcacggga gagacctttc
     8761 catattatta agccctgtca aatacaatat gaagtgagac gggtccgcgt gtatacagac
     8821 cgaactcagc ataacaaaaa tcttcctcta atctgatatt ccgcatctta tccagcacaa
     8881 tattagctac aatttcgatt tcggttttgg caaaagctgc tcctacacag ttatgaatgc
     8941 cggatccgaa agcgagatgc cgagcggcgc cgctaaaagc gctcttgatg ccaagatctt
     9001 cccgatgaat attaaacacg tcaggctgtt caaatgcttc agggtcccgg ttagccgcac
     9061 cgatcataca aaaaacaatc gtatcttttt tgatttccat accgccgacc actgtatctt
     9121 gggacagctg ccgcggaatc agctgaaccg gcggtttata acgcaatgtc tccgcaatgg
     9181 ctctcggaac taacgaacgg tcagccaaaa catcattcat ctgctcagga ttgttgagca
     9241 aatggtagat catcagtgcc agcgtcttat cagccggttc cgttgcggct aacagcacat
     9301 taagaatcag tgcgagtata tccttgtccg acatcgccat gccttcatat tcagaagtac
     9361 ataggatgga aattaaatct gatcccggat tgacgcgacg ttcttcaatg accggcatca
     9421 agtattggga aagctgttcg ctgcaccata acgaatgtgc ccgcgcttca ggagattgag
     9481 agatactcgt gataaaatcg gcaactccgc tgtgccactc agagattttt tcatggtctc
     9541 ttttatccag cccgagcatg tccatcgtga cgcacaccgc aaacgtcttt ccaaaatcat
     9601 tgacgagatc aattttccct ctttcaagat aaggcgctaa caagttttct gcattttgtt
     9661 taatcaatgg agacagatga tccagtgcgt caccgatgaa gcttctcact acaattcttc
     9721 ttttggcaga gtgttctttt ccatgcattt gggccagcac agggcctcgc attactggtt
     9781 cggcacgctc aacaagtgat ttcgtcgtga aaatatccgg atgctgaagg atatagcgga
     9841 catcatgata gcggctgata aaataactgt ctatcgactc ttcataatga accggatctt
     9901 cctcccgcag ttgtgaaaaa taagcatatg gattgttttg aaattgatca gaaagcacac
     9961 taaacaattt aatcgattgg ctcatgtttt actcccccta tcatccttca catgtgatcc
    10021 gtttctcaga aagcatctgc gccatttcct gtacaatgag atacccttga ttcggccgca
    10081 tacaaataga aaattcatgg ttactgatct tctcaccgag cttccacgga cgatgataag
    10141 caaggagcgt ctcttccacc tctaaaatat ccggagctcc gataaaaaac ggcagctcag
    10201 ctatgacata ttccacagcc aaattcagca tatcctcact gacttcttcc atcatcaaac
    10261 tgacgccccg tgcacgcccg attatcgctt cacgagacat gtccaaacaa gcatgtcgaa
    10321 aatgaggctg ctcaaaaaaa gcatgttcaa cttcttgtct caacagctgg taggctttgt
    10381 tatccataaa atcagaaaat gtatgaatcg ccttcggatc cccgccatga gccacaagag
    10441 ctctttctgc aaatctcctg tttcgtgata cctctttcct tacttttcgt tcagcctttc
    10501 ctctcggagt tccaagcgct tctagaagat tagcggcctc atgccccgca agtaaaacag
    10561 aaacgctttt aaattgagct ttcgcccatc caattaatct gtaaatataa tcctctgaaa
    10621 acctgctgtt aaatgggctg atccccacca aaacatgcct gcgccgttca aatatttctc
    10681 tgcagttttc cgttaatggc tcagcaataa aatgcacaga ccttctttcc gttaccattc
    10741 cggtcatctc attcacccct aaaacttatc ccgctttaag tcatcaccct caaatacaag
    10801 atggaatagc tagaacaact gaacaagacg aatggcaggc atcacttaaa gtttccaaat
    10861 gttttttaag attttaataa tcattttcaa tacccatatc atgataaatg tttactagta
    10921 aacattagtc attttatccc ttttccttcc aaacgtcaat atgatctgtg cccaaatatt
    10981 tgaaaataaa tgtaaacatc cattttccac acacttttta cacgcatatt cagcatgaca
    11041 tatcaacaaa tagtctaaac accaacaatt gacaacgaaa acaagaaaag ataaaataaa
    11101 attgttcacc ggtaaacgat ttgttttatt gtttcatcgt aaactattat tcctaagaaa
    11161 gaaggtagcc tatgtctgat ttgacaaaac agatgatata cgacatatac gtgagactgc
    11221 tgcaccttaa tgaacaaaaa gcgaacactt cacttcagca attttttaag gaggccgcag
    11281 aagaggatgt agctgaaatt cccaaaaata tgacaagcat tcacgtcatt gactgcatcg
    11341 gccagcatga acccattaac aatgcgggga ttgccagaaa aatgaactta tcgaaagcga
    11401 atgtaacgaa aatcagcaca aaactgatca aggaagaatt cattaacagc tatcagctga
    11461 cagataacaa aaaagaagtt tattttaaat taacccgtaa aggcagacgg attttcgact
    11521 tacatgagaa actgcataaa aaaaaggagc tggcttttta ccaattcctc gattcatttt
    11581 cacaagaaga acaaaaggct gtattgaagt ttctagagca gttgacgtca acacttgaag
    11641 cagaacaaac cgatgggact ccggacaaac ctgtaaagta aacaaaaagg cggtgtacac
    11701 tttggctcat acaaaatcaa aggcagtatt gatcttatac actgtttgct tcagtgcatt
    11761 ttttgcatct ttaagccaga acatttattc acctattctt ccgatcatta aagaatcatt
    11821 ccatgtttcc acagctatgg tgaacctgtc agtctcagtt tttatgattg tgacagcaat
    11881 aatgcaaatt atattaggag cgatcattga ttttaaaggc gctcggatcg tcttgattac
    11941 cggtattctg gcaacggcag cagccagcat cggctgtgcg gtgactactg actttacttt
    12001 gtttctgata ttcagaatga tacaggcagc cggttccgca gcactgcctc ttattgctgc
    12061 cacaacgatc ggacagctgt ttacaggcaa tgaacgcggg agtgcaatgg gaacgtatca
    12121 aatgctcctg tctgtcgcac cggctattgc cccagttcta ggaggattca taggcggagc
    12181 agccggatac gaagggattt tttggatact tgcggccatc tcgatcgttt tgctggtgac
    12241 aaacagcatc acctttccta aagattctcc aactgaatct atgcagcaag ccaaaggcaa
    12301 tgtgttcgct cattataaat caatatttac aaatcgaaca gggaacgtca ttttgacttt
    12361 aagttttgtt ctctttttca tttattttgc agtaattgtc tacctcccaa tattgctgac
    12421 agagcattac catatagatg tgggtatagc aggactgtta tatttgccgc tggcgctgag
    12481 cacgattgca ggtacgtttc tgtttaaaag aatacaggca aaaatcgggc tgcacacctt
    12541 gtttatcgga agcaatgtga ttgccgcctg cagcatcatt ttatttgctg ttacacattc
    12601 cgtttctctc gttctcatgg ctctgacgct ggcactgttt ggcatctcga tgggggttat
    12661 tcctcccttg tactctacaa tgattactaa tgaatttgag cacaacagag ggagtgcaat
    12721 cggaatgttt aactttatcc gatatacagg catggcagca ggtccgatgg tatctgccta
    12781 cttgctcaca atgatgccgt ctgccatgtc ctttagcctc ctaggccttg gatttgccgc
    12841 attgagcttt tgccttcttc cgccaatgtt ttcgccgcag aagcgcacga aacaaaaaaa
    12901 gcaccacatg taaaaaagct gcctttgcgg gcagcttttt ttattttttt ctaagcggct
    12961 ctaaaatgcc tttttgaatc agcaaatgga agaccgacag gataacggat gcccatatcg
    13021 ctgtgccgaa gccgtcgatt tgaaagctgt cacccattat ggacgcggtc atcataagcg
    13081 tgatggcgtt aatcacaaac aagaagagtc caagcgtcac catggtgacc ggcagtgtaa
    13141 agataattaa aagcggttta atcagcacgt ttaagatcga taaaatgagg ctggcgataa
    13201 tcgctgcccc aatgctgctg atatggatgg agtcaatata tccatcaatg acgattaata
    13261 acaatgcatt gactaaaatg ctgactgccc attttaccat gtcatttcat atccctttct
    13321 gacgggacga taaaaatcca aatgatataa atgagcaaaa ccggcaagac acttgtcata
    13381 atggctaaga tgacagttat gactctgagg agtgatgcgt cccaattgaa atattccgcg
    13441 agacctccga taacaccggc gattttctta tttttttctg agcgataaag cttattcatt
    13501 cgatcacctc tgtgagtact ttagtttgat ggcgcctgtt agtgattctg agaagacggt
    13561 gatgttttga tcatgtgcct gatttgaagt gaacatcatt tctttttgaa tggtatcatt
    13621 tttttccttc agcatctcaa catccattaa ttcatgggac agcgaaccaa gattgctttt
    13681 cagctctgcc ttcactgcga gatcgtccgg aatcgcaagc tccacacttc cagtcgttgt
    13741 ttttgcataa atggagcggc agtctgattc tgttacgtta atcgcaatat ttccgttaaa
    13801 gctttgaacg tcaatggatt cgcttttccc tctgagatcg atcaatccgt taatggtttc
    13861 cgcttctatc gttccgcagc tgtggctggc gagcttaatt tgcccgttgg ccgtttcagc
    13921 tatggctttt tcggctgtta agtgcgagaa cgacagcacg ccgtttgtcg ttttcgctga
    13981 gaactctttt acatgtaaat gttcccctct gaccggaccg ttaaacagct tcactcgaat
    14041 cttatcatac tccttctgag gaatatacaa agtgacattc gttttcatcg tctttttctc
    14101 agtacggata aaaaacttat ttcctttgat ttcacattca atatgctgaa gaaatgcgtg
    14161 tctcgccgca tcctggctat cagcgcgata taccttcgcc tggcattccg ctctgatatc
    14221 gtcatcttcc caaggaacga tattcacgct gccattggca atttgtagct caacactgga
    14281 aaagtcagta tctttaaatt gaaaaatgtg ctgaatatca tacgcatggc caaagtttaa
    14341 atcaaggtca acttccttga ccttttttac agcagaatca atccagtcaa acagcttggc
    14401 tcctaaagac ggcttgccgc tttctttttt tgccgttgta aatggctctt cctcgtcatg
    14461 aacgtgaact gagagcgctg tgattttctc ttctttttcc ttgtagtcgc tgtcaagctt
    14521 ttcaatcagt gtcaaagctt cttgtgctgt aagctttcct tcctcaacaa gcttaaggat
    14581 tcgttccttt tcttgcttca ttacgatctc ctccccattg tctacttatg cttcacgcag
    14641 gaccttaatg ccctgaatca cattccaaat gataatgcca atgacaacta agaaataaat
    14701 aaccgctccc ccgatgatta caaatacagg aagcaagctg tctccatcaa tcgcaacggc
    14761 tccgccaagt aaagcaataa aaaggaacag ccagccgaca aacggaacta tgtgagaaat
    14821 aagagagcgt atcgcatggc gttttgtttc tttctcattt acaacaaaat aagcgacaat
    14881 cggcacaatg attggcgcaa taaacacact gaaataacaa agtgatgcaa taatagcttg
    14941 attacggttc aattcattcc acactcctat tgtgaattgt ccatgacaaa agatcaggga
    15001 cgttttactt atttatacgt ttaaaatctc ttcaggtttc aaattcattc tttagacatg
    15061 ctccgattta cggaaattgg attttaaaaa gttttagaag tagaatcttc cggctatacc
    15121 gattacaata tgtaaaaagc ttacggcaac caaggaggct ggacaagcaa tggagaaaga
    15181 tcccagtgac tatacagtaa cacaagaatc agtgttaaag ctgattcatg aacaaaaaag
    15241 aatgaatcgt gaaatgatcg ccgagcttga gcagattcac ggacccttcc cgatctctca
    15301 cgatatccaa tatataaaag tgctgctcga cagcagcaac acgcatattg ttcaagattt
    15361 aatgagtgta agtaagcagc tgtataagaa gactttgtaa ttgcggaaaa catatcattc
    15421 attataactg aaacaaagtt aaaggttaga tgacaatatc ttgccttttt tgttttgaaa
    15481 aaacaagtga ttccctggct gtcacaaaat gattgtaaca aaaaaagcct gccggcacat
    15541 ccagttaatg gactctgtcg gcaagctgta actatcagaa agagatagcc gttcaaaatt
    15601 aacgtaacaa ttgaagcacg ttttgcggct gttggtttgc ttgagcaagc atcgcttgag
    15661 aagcttgaga aagaatgttg ttctttgtga aatcgctcat ttctttcgcc atgtcaacgt
    15721 cacggatacg agactcagca gctgttaagt tttcagaaga agcaccaagg ttgttgattg
    15781 tgtgctctag acggttttgc accgcaccaa gtttagcacg ttggtttgaa acacttgtaa
    15841 ttgcactatc gattttaaca atctcagtgt caaaactaaa atcatctgct gaaacatcga
    15901 taccttttac tcctaaagtt gttgcatcca tgttaccaat tgtgacatta atattttggc
    15961 cagcatttgc accaatttgg aatgtaaatc ctgctgtttc actaccgtct aaaagttttt
    16021 ttccgttaaa ttcagtacgt tgagaaattc catcgatctc ttcagttaaa gcatccattt
    16081 catctttaat tgcagttaaa tcttcagttt gctgtgtacc tgtgtttcct gcttgaacag
    16141 taagttcacg catacgttga aggattgcat gggtttcagt caatgcaccc tcagctgttt
    16201 ggataagaga aattccatct tgagagtttt tagaagccat ttcaagacct ctgatttgtc
    16261 ctctcatttt ttcagaaatc gcaagacccg ctgcgtcatc tcccgcacgg ttgatgcgaa
    16321 gaccagaaga aagtttctcc atgttttttg agctcgcact gttgtttgaa gacaaacggt
    16381 ttagtgtgtt aagcgctgca atattgtggt tgattctcat gtgaaaaata cctccatgtg
    16441 tatatgtttt tgaaatcaaa gacttccttt tctttgattt ctggcgtatc agattccaat
    16501 aaagcttcta acctatgtag attcagttat tgcttaagga tgcctttaag ccccttaata
    16561 gggtttgtct gcttaatttg gaaatcatca tccgcttttc aattcatata tcgacagcaa
    16621 tttgtaaatg tttaggcgtt ttcccaaaga aattttacca aagacgaatc taaacctatt
    16681 atgccgtaaa gaaaaagctt gcctgctaag cccaaggatg gacttgctga caagctgtcc
    16741 cctcttaaca gagggtttta agatgtagct gtttctttcg ctttcaagag cgatttcatg
    16801 cgtgttttgt cacgttcgat aacaggcttc aaataacggc ctgtatacga ttcttccact
    16861 tcagtgattt cctcaggcgt tcctgacgcg acaatggttc cgcccccggc tccgccttcc
    16921 gggcccaaat ccacaatgta atcggccgtc ttaatgatat caaggttgtg ctcaataacc
    16981 agtacagtgt ctccgttgtc taccaaccgt tgcagcacga caagaagcct ggcgatatcg
    17041 tcgacatgca aacctgtcgt cggctcatct aaaatgtaga gcgtgcgtcc ggtcgagcgt
    17101 ttgtgcagct cagatgcgag cttcacgcgc tgcgcttctc cgcctgacaa ggtcgtcgcc
    17161 ggctggccga gcgtaatata acctaaacca acatcataaa gggtttggag cttgcgtttg
    17221 attttcggga tattttcaaa gaaagaaaga gcatcttcaa ctgtcatatc aagcacatca
    17281 gagatgcttt ttcctttgta cgtcacttca agcgtttcac ggttatagcg tttgccgtga
    17341 cacacctcgc atggaacgta tacgtcagga aggaagtgca tttcaatttt aataatcccg
    17401 tctccgcggc aggcttcaca tcgtccgccc ttcacgttga agctgaaacg gcctttttta
    17461 tagccgcgga ccttcgcttc atttgtctgc gcgaatacat cacgaatgtc atcaaataca
    17521 ccggtgtatg tcgcagggtt ggatctcggc gttcttccga ttggcgcctg gtcaatgtca
    17581 atgactttgt ctaaatgatc caaaccttta atctctttat ggctgccggg tttcgctttc
    17641 gctttatgaa gcttttgcgc cagcgcctta tgcaaaattt cattaacgag tgtactcttt
    17701 cctgaaccgg aaacacctgt aactgctgta aacgtcccaa gcgggaactt ggcattcact
    17761 tttttcaggt tgttttctga agcaccttta atttcaatgt aacgtccgtc cggctttctt
    17821 ctttcaggag gcaatgggat aaactttttc cctgataaat agctgcccgt taatgagttt
    17881 ggatcttcca tcacttcttc cggcgtaccc gcagatatca cctgtccgcc gtgaatgccg
    17941 gctcccggtc caatatctat taaataatct gctgccatca tcgtgtcctc atcatgttcg
    18001 acaacaatca gcgtgttccc gaggtctctc atatttttca gagcgctgat caagcggtcg
    18061 ttatcacgct gatgcagacc gatagacggc tcatctaaaa tataaagcac accggataag
    18121 cgcgagccta tttgagtcgc cagcctgatg cgctgcgcct ctcccccgga caatgtaccc
    18181 gctgccctgc tcaatgtcag gtaatcgaga ccgactttgt ccagaaagct taagcgctcc
    18241 acaatttcgc gcaaaatcaa attggcgatc tgcatatcct tctcagaaag ggtaaggtct
    18301 ttaaagaagg caagtgcgtc ggcgacagac agctcggtaa tttttccaat gtggcggccg
    18361 tcaatcagta cggcaagcgc ctctttcttt aaccgatagc ctttgcacgt cggacaagac
    18421 ttctgagaca tatactgctc catttgctca cgtatgaaat cggagcctgt ctccttatag
    18481 cgtctttcaa tgttgcgcaa tacgccttca aattgaattt caccttcgcg gatttgtcca
    18541 aaatcatttt catatcggaa ataaatcagg tcatctccgc tgccgtacag cactttatcc
    18601 agttgatgct tcggcaaatc tttgaccggc acatccatat caatcccgta gtgggtgcag
    18661 actgcctcaa gcagctgagg ataatattgt gagctgatcg gtgtccaagg ggcgaccgca
    18721 ttctccttca atgacaaatc ttgattgggg atgacaagat cggcatccac ttcaagcttc
    18781 attccgagac cgtcacacgt cggacacgcc ccgaacggac tgttaaacga aaacagacgc
    18841 ggctcaagct caccaattga aaatccgcag tgcggacagg catgatgctc gctgaacatc
    18901 agctcttcct caccgatgac atcgatcata acccgtcctt cacctaaacg gagcgccgtt
    18961 tccaatgaat cagacagccg ggctgccacg ccttctttca cgacaatccg gtcaatgaca
    19021 acctcaatgg aatgcttctt gttcttttct aattcgatat cgtcggaaag ctcagccatc
    19081 tcgccgtcaa ttctgactct gacatagcct tgttttctaa tctgttcaag cactttgaca
    19141 tgagcgcctt ttcggcccga gacaatcggc gccagcacct gaagcttcgt ccgttccggg
    19201 tattccagaa ttctgtccac catttgctcg atggtctggg atgtaatctc aattccgtgc
    19261 tccggacaat gaggcttccc tactctcgca tataaaagac gcagataatc ataaatctca
    19321 gttaccgtac cgacagtaga cctcggatta cggcttgttg ttttctgatc aatgctgatg
    19381 gcgggagaga gcccctcaat tgcatccaca tcaggcttat ccatttgccc taaaaactgg
    19441 cgggcataag cagacagcga ctcgacatac cgtctctgtc cttcagcata tatcgtgtca
    19501 aaggcaaggg aggatttacc tgatccggac aaacccgtga caacgacaag ctgatctctc
    19561 ggaatcgtta catctatatt tttcaggtta tgcgccctgg ctcccttcac ctctatccga
    19621 tccatagcca tttgttcatc atccttccgc ttttagctct aaaagtaaat cgcgaagctc
    19681 cgcggctctc tcaaagtcaa gcgccttggc ggcttccttc atttcatgct ccatctgttc
    19741 aacgactttc tggcgttctt tcttcgtcat tttcgacagc ttcggcgcag cttttgtttt
    19801 gtattccgct ttatcctcag ctgctactgt ggcacgaatg acatcgcgaa tttctttatt
    19861 tatcgttttc ggcgtaatgc cgtgctcttc attaaaccgc tcctgctgct cgcggcggcg
    19921 ttttgtttca ttaatcgcaa tttccataga cttcgttatt ttatcggcgt acataatgac
    19981 gcggccttcc gcatttctcg ctgcccgtcc gatggtctgg atcagcgacc gctccgaacg
    20041 aaggaaacct tccttatccg catccaaaat cgcaacaagg gatacttcgg gaatgtccaa
    20101 accttccctc agcaggttga tgccgacaag cacatcgtac ttgccaaggc gcaggtcgcg
    20161 gataatttca atccgttcaa gcgtcttgat ttcagaatgc agatagttta ctttaatgcc
    20221 gatttccttt aaataatcgg tcagatcctc tgacatcttc ttcgtcaaag ttgtcacaag
    20281 cacccgttca tttcgctcga ttcttgcttg aatttcgccg atcaagtcgt caatctgacc
    20341 ttcgattgga cggacgtcaa taagcgggtc aagaagcccc gtcggacgaa tgatctgttc
    20401 gaccatttca tctgtatgtt caatctcgta cggccctggt gttgcggata catacacgat
    20461 attgtgcata tgcttttcaa attcctcaaa acgaagcgga cggttatcaa gcgctgatgg
    20521 aagccggaag ccgtgatcca cgagcacctg tttccgcgcc tggtctccgt taaacatgcc
    20581 gcgcacctgg ggaattgtca catgcgactc atccaccacg atcataaaat cgtctgggaa
    20641 ataatcaaga agcgtatacg gcgttgaacc tggaggccgc agagtcaaat gtcttgaata
    20701 gttctcgatg ccggagcaga aacccatttc acgcatcatt tcaagat
//
