LOCUS       NZ_JAGPXN010000058      1425 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Micromonospora sp. C31 C31_contig_58, whole genome shotgun sequence.
ACCESSION   NZ_JAGPXN010000058
VERSION     NZ_JAGPXN010000058.1
KEYWORDS    WGS; RefSeq.
SOURCE      Micromonospora sp. C31
  ORGANISM  Micromonospora sp. C31
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Sahin,N., Saygin,H. and Ay,H.
  TITLE     Plant growth-promoting actinobacteria from halophytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Sahin,N., Saygin,H. and Ay,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2021) Molecular Biology and Genetics, Ondokuz
            Mayis University, Kurupelit, Samsun 55139, Turkey
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. APR-2021
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018070575.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:57:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,539
            CDSs (total)                      :: 5,480
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 23 of 123
            Pseudo Genes (incomplete)         :: 110 of 123
            Pseudo Genes (internal stop)      :: 3 of 123
            Pseudo Genes (multiple problems)  :: 12 of 123
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAGPXN010000058.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1425
                     /collection_date="2018-07-24"
                     /db_xref="taxon:2824876"
                     /geo_loc_name="Turkey: Samsun"
                     /host="Atriplex sp."
                     /isolation_source="ENVO:01001001"
                     /lat_lon="41.18 N 36.15 E"
                     /mol_type="genomic DNA"
                     /organism="Micromonospora sp. C31"
                     /strain="C31"
                     /submitter_seqid="C31_contig_58"
     gene            <1..>1425
                     /locus_tag="KBX06_RS27415"
                     /old_locus_tag="KBX06_27415"
     region          1..1425
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..1425
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1425
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4:>1425](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>1425
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             5..>1425
                     /NRPS_PKS="Domain: PKS_AT (272-474). E-value: 3.6e-73.
                     Score: 238.5. Matches aSDomain:
                     nrpspksdomains_KBX06_RS27415_PKS_AT.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285629.1"
                     /locus_tag="KBX06_RS27415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27415"
                     /product="type I polyketide synthase"
                     /protein_id="WP_210839947.1"
                     /sec_met_domain="mod_KS (E-value: 5e-91, bitscore: 291.7,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 6e-75, bitscore: 238.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRGTAVNQDGASNGLTAPNGPSQQRVIRQALANAGLGVADVDVV
                     EAHGTGTTLGDPIEAQALLATYGQGRDEPLLLGSVKSNIGHTQAAAGVAGIIKMILSMR
                     HGVVPASLHVDAPSSHVDWSSGAVELVTESRAWPGVGRARRAGVSSFGISGTNAHVIIE
                     QPEPGLLALSAGESAVAESVVASGVPVPWVVSGRSEGALVGQAVRLASFVRDHAGLSPV
                     DVSWSLVSTRAALEHRAVVWGSDVDELVAGLSAVSGGRLSGVVSAGRRAVLFTGQGSQR
                     AGMGRELYEAFPVFASAFDGVCAQFDGLLPRGLREVVFAEAGSAEAALVDQTVFAQAGL
                     FAVEVALWELLSSWGVRVDFLAGHSIGEVTAAYVAGMLSLADACTLVAARGRLMQALPA
                     GGVMAAVGASEEAVAELIAVSGVAVDIAAVNGPASVVVSGAADEVASVVGTSRERGWRV
                     KELSVSHAFHSRLMDP"
     CDS_motif       58..78
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0001"
                     /evalue="4.30E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="24"
                     /protein_start="17"
                     /score="-1.7"
                     /tool="antismash"
                     /translation="APNGPSQ"
     CDS_motif       139..183
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0002"
                     /evalue="6.60E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="59"
                     /protein_start="44"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       238..282
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0003"
                     /evalue="1.20E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="92"
                     /protein_start="77"
                     /score="14.1"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       547..594
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0004"
                     /evalue="1.40E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="196"
                     /protein_start="180"
                     /score="-0.3"
                     /tool="antismash"
                     /translation="ESVVASGVPVPWVVSG"
     aSDomain        823..>1425
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KBX06_RS27415_PKS_AT.1"
                     /evalue="3.60E-73"
                     /label="KBX06_RS27415_PKS_AT.1"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="474"
                     /protein_start="272"
                     /score="238.5"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRAGMGRELYEAFPVFASAFDGVCAQFDGLLPRGLREV
                     VFAEAGSAEAALVDQTVFAQAGLFAVEVALWELLSSWGVRVDFLAGHSIGEVTAAYVAG
                     MLSLADACTLVAARGRLMQALPAGGVMAAVGASEEAVAELIAVSGVAVDIAAVNGPASV
                     VVSGAADEVASVVGTSRERGWRVKELSVSHAFHSRLMDP"
     CDS_motif       826..873
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0005"
                     /evalue="8.40E-07"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="289"
                     /protein_start="273"
                     /score="21.2"
                     /tool="antismash"
                     /translation="FTGQGSQRAGMGRELY"
     CDS_motif       1081..1191
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0006"
                     /evalue="6.50E-19"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="395"
                     /protein_start="358"
                     /score="60.1"
                     /tool="antismash"
                     /translation="DFLAGHSIGEVTAAYVAGMLSLADACTLVAARGRLMQ"
     CDS_motif       1285..1323
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0007"
                     /evalue="1.90E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="439"
                     /protein_start="426"
                     /score="14.6"
                     /tool="antismash"
                     /translation="AAVNGPASVVVSG"
     CDS_motif       1387..1413
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KBX06_RS27415_0008"
                     /evalue="1.20E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="KBX06_RS27415"
                     /protein_end="469"
                     /protein_start="460"
                     /score="8.8"
                     /tool="antismash"
                     /translation="LSVSHAFHS"
ORIGIN
        1 cggtggtgcg tggcacggcg gtgaaccagg acggtgcgtc gaacgggttg acggcgccga
       61 acggtccgtc gcagcagcgg gtgatccggc aggccctcgc gaacgccggg ttgggtgtcg
      121 ccgacgtgga cgtggtggag gcgcacggta cgggtacgac gttgggtgat ccgatcgagg
      181 cgcaggcgtt gttggcgacg tacgggcagg gccgggacga gccgttgttg ttggggtcgg
      241 tgaagtcgaa catcggtcac acgcaggccg ccgcgggggt ggcggggatc atcaagatga
      301 tcttgtcgat gcggcatggc gtggtcccgg cgtcgttgca cgtggacgcg ccgtcgtcgc
      361 acgtggactg gtcgtcgggt gcggtggagc tggtgaccga gtctcgggcg tggccgggtg
      421 tggggcgtgc ccggcgggcg ggtgtgtcgt cgttcggcat ctcggggacg aacgcgcacg
      481 tgatcatcga gcagccggag ccgggtcttc tggcgctgtc ggcgggtgag tcggcggttg
      541 cggagtccgt cgtggcgtcg ggggtgccgg tgccgtgggt ggtgtcgggg cgctcggagg
      601 gtgctctggt gggtcaggcg gtgcggttgg cgtcgttcgt gcgtgatcat gcgggtctgt
      661 ctccggtgga tgtgtcgtgg tcgttggtgt cgacgcgggc ggcgttggag caccgtgcgg
      721 tggtgtgggg ttcggacgtc gacgagttgg tggcgggttt gtcggcggtg tccgggggtc
      781 ggttgtcggg tgtggtgtcg gcgggtcgtc gggcggtgtt gttcacgggt cagggttcgc
      841 agcgtgccgg tatgggtcgg gagttgtacg aggcgtttcc ggtgttcgcg tcggcgttcg
      901 acggggtgtg tgcgcagttc gatggtctgt tgccgcgtgg gttgcgggag gtggtgttcg
      961 cggaggcggg cagtgccgag gcggctctgg tggatcagac cgtcttcgct caggcggggt
     1021 tgttcgccgt cgaggtggcg ttgtgggagt tgctgtcgtc gtggggtgtg cgggtcgact
     1081 ttttggcggg tcattcgatt ggtgaggtga cggcggcgta cgtggcgggg atgctctccc
     1141 tcgctgacgc gtgcactctg gtggcggcgc ggggtcgtct gatgcaggcg ttgccggctg
     1201 gtggggtgat ggcggcggtg ggtgcgtcgg aggaggctgt cgccgagttg atcgcggtgt
     1261 cgggtgtggc ggtggatatt gcggcggtga atgggcctgc gtcggtggtg gtgtcgggtg
     1321 ccgctgatga ggtcgcgtcg gtggtgggga cttctcgcga gcggggttgg cgggtcaagg
     1381 agttgtcggt cagtcacgcg ttccattcgc ggttgatgga tccga
//
