LOCUS       NZ_JAGPXN010000041      9315 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Micromonospora sp. C31 C31_contig_41, whole genome shotgun sequence.
ACCESSION   NZ_JAGPXN010000041
VERSION     NZ_JAGPXN010000041.1
KEYWORDS    WGS; RefSeq.
SOURCE      Micromonospora sp. C31
  ORGANISM  Micromonospora sp. C31
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Sahin,N., Saygin,H. and Ay,H.
  TITLE     Plant growth-promoting actinobacteria from halophytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Sahin,N., Saygin,H. and Ay,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2021) Molecular Biology and Genetics, Ondokuz
            Mayis University, Kurupelit, Samsun 55139, Turkey
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. APR-2021
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018070575.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:57:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,539
            CDSs (total)                      :: 5,480
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 23 of 123
            Pseudo Genes (incomplete)         :: 110 of 123
            Pseudo Genes (internal stop)      :: 3 of 123
            Pseudo Genes (multiple problems)  :: 12 of 123
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9315
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAGPXN010000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9315
                     /collection_date="2018-07-24"
                     /db_xref="taxon:2824876"
                     /geo_loc_name="Turkey: Samsun"
                     /host="Atriplex sp."
                     /isolation_source="ENVO:01001001"
                     /lat_lon="41.18 N 36.15 E"
                     /mol_type="genomic DNA"
                     /organism="Micromonospora sp. C31"
                     /strain="C31"
                     /submitter_seqid="C31_contig_41"
     region          1..9315
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..9315
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9315
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[337:6790](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      338..6790
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            77..220
                     /gene="fxlA"
                     /locus_tag="KBX06_RS27135"
                     /old_locus_tag="KBX06_27135"
     CDS             77..220
                     /codon_start=1
                     /gene="fxlA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LD_lanti_pre"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018829768.1"
                     /locus_tag="KBX06_RS27135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27135"
                     /product="FxLD family lanthipeptide"
                     /protein_id="WP_210839879.1"
                     /sec_met_domain="LD_lanti_pre (E-value: 4.7e-12, bitscore:
                     34.7, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAQTQNGRKLPNHPLSLLEVADVAGLVNLTDDGCGSTCTACTTN
                     VA"
     gene            338..3412
                     /locus_tag="KBX06_RS27140"
                     /old_locus_tag="KBX06_27140"
     CDS             338..3412
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018805746.1"
                     /locus_tag="KBX06_RS27140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27140"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_210839881.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.5e-203,
                     bitscore: 665.6, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 8.4e-22,
                     bitscore: 66.7, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.2e-23, bitscore:
                     73.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTALVRAVAHPAIELPPWPDLTEPRPDLVPSWVTWLRQTWAIDT
                     VAEAITLSSPVLAAQVSKLCAGETASVRETRRTVQAVVRYAQRLLGRPTPFGLLAGVAP
                     AVFGSRAPTRWGTAHQAIARAGAGWLTDVITHIESCPELLSRLVVVANSTLTVRDDRLI
                     VPYQPQPGLDGHLGAAEVSLRHSAPVRAVIAAARSPIRVEVLASQLRGAFPQASAAAVT
                     AMLTQLIAHRALITSLHAPSTEPDALGYLLSQLDEAGDGTGTPVAEVIAALREIHAGLR
                     RHNTAQPGDAPAARRLVASRIRSLAISEHEPLAVDLRLDASVTLPDQVAREVEQATLVL
                     ARLSVYPYGTAAWRAYHQRFYERYGIGSLVPVRDVVDPDSGIGLPDGYPGSPAPDPRSP
                     MSRRDEALLQMAQRAALDAATEVVLDEATIAQLDLGPQRLRLPPHLEACVRVQAADGKD
                     LARGRFTVEVTSISRAAGGLTGRFLSVLRPTDAGWLAAALSELPAGDRDTVAAQVSFPP
                     LDPGTAHVARAVQVLPTVISLAEHRWPSRSVLTADDLTVGCDGRRLYLAAPDRGHRIEA
                     VGMHALNLRTHTPPLARFLIELGRAQCAQVTAFDWGAAARTKLPFLPRVRYGRAILTPA
                     TWRLEAAELPDRHQPWYAWDDALDEWRARRRLPRLVHLVEADRRLPLDLDESAHRVLLR
                     AHLLTSPRAVLVEAPTPEDLGWCGSRPHEVIVALHATEPPPWPPLPTPTPARVIGRDQG
                     QSPAASRILLAKLYGDIRRQDLLLAEHLPRLIAEWDAPPDWWFLRFRDPDQHLRLRIAL
                     SNASAFGPAAERVSAWADDLRRRGLLREVQYATSYPETGRWGSGPAMQAAEAVFIADSH
                     AVLTQLRQTVRPSRQALVAAHTAAIAVAYTGSLETGMRWLVDHVPATAPQPVPRPLFRE
                     AVRLAQPGGDWAALRAVAGGSAIVDAWKNRDVALAVYRTHLPGPHTDGIDSHDVLGSLM
                     HAHFIRAVGIDFDDEDQCRYLARAAALAYFARSRS"
     aSDomain        782..1060
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KBX06_RS27140_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="3.40E-21"
                     /identifier="RREFam001.1"
                     /label="KBX06_RS27140"
                     /locus_tag="KBX06_RS27140"
                     /protein_end="241"
                     /protein_start="148"
                     /score="66.7"
                     /tool="antismash"
                     /translation="VVANSTLTVRDDRLIVPYQPQPGLDGHLGAAEVSLRHSAPVRAVI
                     AAARSPIRVEVLASQLRGAFPQASAAAVTAMLTQLIAHRALITSLHAP"
     gene            3409..4704
                     /locus_tag="KBX06_RS27145"
                     /old_locus_tag="KBX06_27145"
     CDS             3409..4704
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019901917.1"
                     /locus_tag="KBX06_RS27145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27145"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_210839883.1"
                     /sec_met_domain="LANC_like (E-value: 1.5e-47, bitscore:
                     150.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLAYESHELAVAVADLLAHPDDPSLPIHQPWWRQSLALGVPGIA
                     LLHVELAAAGLRPWQRAHHWLAAATSGPVTTGPDSHPFHGAPALAHVLASAAVHQPGSY
                     ARALEGLDTAIAADARRRVAAAHARIDAGELPALAEFDAIRGLTGIGAYLLRRSSGGQE
                     LRAVLEYLVRLTSPLHREGEMLPGWWTGNGPNGRRDDRFPGGHGNTGMAHGIAGPLALL
                     ALAALRGVVVQGQLAAIATICSWLDRWRSTTDAGPIWPYWVTRSQLRAGHASPSSAQRP
                     SWCYGTAGLARAQQLAALATGDLVRRVAAEHALARALTDRRQLAATTDPSLCHGYAGLA
                     HIAHRVAADALPGNAARLNARVPHLLNRIDPAGAGPRRTAAALIRDVPGPGLLEGATGV
                     ALAALSCSTSSVPDSSWDFCLLTARPTQREDV"
     gene            4709..6790
                     /gene="fxlM"
                     /locus_tag="KBX06_RS27150"
                     /old_locus_tag="KBX06_27150"
     CDS             4709..6790
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fxlM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Methyltransf_3"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04364.1"
                     /locus_tag="KBX06_RS27150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27150"
                     /product="methyltransferase, FxLD system"
                     /protein_id="WP_210839884.1"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.8e-34, bitscore:
                     108.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PCMT (E-value: 1.1e-46, bitscore: 148.1,
                     seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="Methyltransf_3 (E-value: 9.9e-09,
                     bitscore: 23.6, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTPAWRQYTVEFSTPAAAEQIAATVLAPALAAAQDAGVLHGWWY
                     VRKYPSWRWRYLADDPTSHLFENLLETLAVDDRIVTWTRGLYEPETLAFGGSAGMHVAH
                     DLFHQDSRQLLLGPGFAESAMLGRRELAILLCSVLMRSGGLDWYEQGDVWTKVAELRPA
                     PALPAESATAAALSRAIDRLMSVDIRSLSRPGSDGPLAGYGAWLDAFEAAGQALADLAR
                     HGRLRRGLRAVVAHHVIFHANRMGLSVRDQSTLAALAVRNVFDPIRRSTVSTSAPIPPA
                     TSVDEMTTLREPADLRSELTDRLRTENIIRTPSVNAAIRQTPRHLFLPGVPLEQAYADA
                     PVYTKTDGRGASISAASQPRIVAMMLEQLDARPGHRIMEAGAGTGYNAALLAAIVGETG
                     RVVTIDVDDDLIDGARKHLAAAGVANVEVVHGDGALGHPDAAPYDRIIATVGAWETPTT
                     WLEQIAPDGRLVVPLRLRGAASRSIVFERYEGGWRDVGSELAVFMPLRGIADDARRLVT
                     LTDEQDVTLQVHKDQSADAAALAGVLDAERHETWTGVIFPPMVPFEWLDLWLACRLDNA
                     IMRMNVKPEAIERGTVSPMFPWGAMATTRGADLAYLTLRPAPPAADGGKLYEVGVIGHG
                     PSGEGLAQHVADEIRVWDAQYRSRTVHLEIPDSPTEANPSAGRFLLPRPHHPITVTWQ"
     gene            6793..7965
                     /locus_tag="KBX06_RS27155"
                     /old_locus_tag="KBX06_27155"
     CDS             6793..7965
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019901919.1"
                     /locus_tag="KBX06_RS27155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27155"
                     /product="hypothetical protein"
                     /protein_id="WP_374225682.1"
                     /transl_table=11
                     /translation="MGPTSHVLRRFPLVARPRPAAKALTSRVDDLRVLAERADHEGDLA
                     AASAVYNQAALIASDCGQPELARHWCHEHADAYLHALPLGAQAARHALEPLVNLARLHI
                     RDGNGDAALGLLNNLFQALSSRTDTLIDGHPVPASRLTRTDDDHREVRQWLWGVLLADG
                     SRALTGAGRWKEAYEHLQRHNGIGQRMLDGRQVAVIAHATSGEIERARAIITETVRGEP
                     WEEAVTACLAYLCARAGGEPDGAYLDTLLRTYRRLTPTPSLAVFHTRLGLTVLDAAGGV
                     DQPGVRSLAADLIDQALAYGDAYVARELLGHPGICAVIGAGREAGLVDIMQEGMMHAQL
                     PSVARKRLLQSLTTSRAVITRGSLVPGPRLEPGGVGRQIGGVATEEALPG"
     gene            complement(7868..8326)
                     /locus_tag="KBX06_RS27160"
                     /old_locus_tag="KBX06_27160"
     CDS             complement(7868..8326)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074343.1"
                     /locus_tag="KBX06_RS27160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27160"
                     /product="hypothetical protein"
                     /protein_id="WP_210839888.1"
                     /transl_table=11
                     /translation="MTTDSPWLIGSWMSVVQQPNLVDELLGQEDEYAGVALLALTLNHH
                     DPDVVLPRIKRGLGSMRAQTRANALQSLGHHARLHGSIDVESLARLRLALRHRTVLGGY
                     EIRGYAANAASDVGMFVSRAGLPRWLRRRFAGPRHPVRASARAQVNPS"
     misc_feature    complement(8042..8044)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8410..8907)
                     /locus_tag="KBX06_RS27165"
                     /old_locus_tag="KBX06_27165"
     CDS             complement(8410..8907)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722406.1"
                     /locus_tag="KBX06_RS27165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KBX06_27165"
                     /product="hypothetical protein"
                     /protein_id="WP_210839889.1"
                     /transl_table=11
                     /translation="MSIRQLLGLYSRILNELIQRGVVRSRNAPAGDLAESVVAKAYRGE
                     LAPQSEKSWDVRAAGGTLLQVKCRVIEPGSRRTQVYSPFRSWSFDACVFVILNSVTYDI
                     EQAVEVPAGQVKTAARASSWVAGHRITVSQTRAGIDGAVDVTARLREAYEAMDRREQTQ
                     VV"
     misc_feature    9166..9181
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
ORIGIN
        1 ggatacgccg cacgctcacc catgatcagc ccaacgccgg ccggacctac cggttacgac
       61 cacggctcac attcacatgc cggcccaaac ccagaatgga cgaaagctac cgaaccaccc
      121 tctcagcctg ctggaggtgg cagacgtcgc cggtctggtc aacctgaccg acgacggctg
      181 cggcagcacc tgcaccgcgt gcaccaccaa cgtcgcctga tcaacgggct ttgaaaggcc
      241 ggccggcgcc gccacgacgg tcggcgccgg ccgggcaccc ccgtctcacc gaggtgatca
      301 agtgagccgc tccgtggagt cttctgccta tcggtcgatg ggcaccgcgc ttgtccgcgc
      361 cgtcgcccat ccggccatcg agttgccccc gtggccagac ctgaccgagc cccgccccga
      421 cctcgtgccg tcctgggtta cctggctacg acagacctgg gccatcgaca ccgtggccga
      481 ggcgatcacg ctctcgagcc cggtgctcgc ggcgcaggtc agcaagctct gcgcgggtga
      541 gaccgcttcg gtacgcgaga cacggcggac ggtccaggcc gtcgtccggt atgcccagcg
      601 tctgctgggt cgccccacgc cgttcgggct gctcgcgggc gtggcacccg ctgtgttcgg
      661 ctcgcgagca cctacccgat ggggcacagc ccatcaggcg atcgcccggg caggcgctgg
      721 ctggctcacc gacgtgatca cccatatcga aagctgcccg gagctgctct cccgtctcgt
      781 cgtggtcgcg aacagcacgc tgacggtccg cgacgaccgg ctgatcgtcc cctaccagcc
      841 gcagcctggg ctcgacggcc atctgggagc ggcggaggta tccctacgcc attcagcgcc
      901 ggtacgggcc gtcattgcgg cagcccgcag cccgatccgg gtggaggtcc tcgcctcgca
      961 gctccgtgga gcgtttccgc aggcgtcggc agcagccgtc acggcgatgc tcacccagct
     1021 gatcgcccac cgcgccctca tcacgagcct gcacgctccc agcacggagc cggacgcgct
     1081 cgggtacctg ctgagccaac tcgacgaggc cggcgacgga acgggtacgc ccgtcgctga
     1141 ggtcatcgcc gctctccgcg agatccatgc cgggctacgg cggcacaaca cggcgcagcc
     1201 gggcgacgct ccagcggccc gacgcctggt ggcctcgcgg atacggagtc tcgcgatctc
     1261 cgagcacgag ccgctcgccg tggatctccg gctggacgcc tcggtgacgc tccccgatca
     1321 ggtagccaga gaggtggaac aggccacgct ggtgctggcc cggttgagcg tgtaccccta
     1381 cggcactgcc gcctggcgcg cctaccacca gcgcttctac gagcgctacg gaatcggctc
     1441 gctggtgccg gtgcgggatg tggtcgaccc ggacagcggc atcgggctgc cggacggcta
     1501 cccgggcagt cccgcacctg atcctcgctc accgatgtcc cgccgcgacg aggcactgct
     1561 gcagatggca cagcgtgcgg cactggacgc cgcgaccgag gtcgtcctcg acgaggcaac
     1621 gatcgcgcag ttggacctgg gaccgcagcg gctacgtctt cctccgcacc tggaggcatg
     1681 cgtacgggtg caggccgccg acgggaagga cctggcccgc ggcaggttca cggtcgaggt
     1741 cacctccatc tcccgggccg ctggcgggct caccggccgg ttcctcagtg tgctgcggcc
     1801 gaccgacgcg ggatggctgg ccgctgccct gagcgaactg cccgccggcg accgggacac
     1861 cgtcgccgcc caggtgtcgt tcccgccact ggatcccggg accgcgcacg tcgcgcgcgc
     1921 cgttcaggtg ctgccaacgg tgatcagcct ggccgagcac cgctggccca gccgatcagt
     1981 gctcaccgcc gacgacctga ccgtgggttg cgacggccga cgcctgtacc tcgcggcacc
     2041 ggaccggggc catcggatcg aggcggtcgg catgcacgcg ctgaacctgc gtacccacac
     2101 tcccccgctg gcccggttcc tcatcgagct cgggcgggcc cagtgtgccc aggtcacggc
     2161 cttcgactgg ggcgccgctg cccggacgaa gctgcccttc ctcccccggg tgcgctacgg
     2221 gcgggcgatt ctgacgccgg cgacctggcg gttggaggcc gccgagctgc ccgaccggca
     2281 ccagccctgg tacgcctggg acgacgccct cgacgaatgg cgggctcgcc ggcgactgcc
     2341 gcgcctggtg cacctggtcg aggccgaccg tcgtctaccg ctggatctcg acgagtcggc
     2401 ccaccgcgtc ctgctgcgcg cccacctgct cacctcgccg cgagcggtcc tcgtggaggc
     2461 ccccacgccg gaggacctcg ggtggtgcgg cagccgcccg cacgaggtca tcgtcgcgct
     2521 gcatgccaca gagccgccac cctggccgcc gttgcccaca ccgaccccgg cccgggtcat
     2581 cggacgcgat caggggcaga gtccagccgc ctcgcggatc ctgctcgcga aactgtacgg
     2641 agacatccgc cgtcaggacc tgctcctggc tgagcacctg ccccggctga tcgccgagtg
     2701 ggacgccccg cccgactggt ggttcctccg gttccgcgac ccggaccagc acctacggct
     2761 ccgcatcgcg ctgtccaatg cctcggcgtt cgggccggcg gccgagcgcg tcagcgcgtg
     2821 ggccgacgac ctgcgacggc ggggcctgct gcgggaggtg cagtacgcca cctcctaccc
     2881 ggagaccggc cggtggggat ccgggcccgc catgcaggcc gccgaggcgg tgttcatcgc
     2941 cgactcccac gccgtactca cgcagctgcg ccaaacggta cggccaagcc ggcaggcgct
     3001 ggtcgccgcg cacaccgcgg ccatcgccgt cgcctacacc ggcagcctcg aaaccggaat
     3061 gcgctggctg gttgaccacg tgcccgccac ggcaccccag ccggtccccc ggccgctgtt
     3121 ccgcgaagct gtccgcctcg cccagcccgg tggtgactgg gcggcgctgc gtgcggtcgc
     3181 cggaggctcc gccatcgtcg acgcctggaa gaaccgggac gtcgccctcg ccgtctaccg
     3241 gacccaccta cccggcccac acaccgacgg catcgattct cacgacgtcc tcggctcgct
     3301 catgcatgcg cacttcatca gagcagtcgg catcgatttc gacgacgagg accaatgccg
     3361 ctacctcgcc cgggccgccg ccctggccta cttcgcccgg agccggtcat gagcctcgcg
     3421 tacgagagcc acgagttggc cgtcgcggtc gccgatctcc tcgctcaccc cgacgatccc
     3481 agcctgccga tccaccaacc ctggtggcgg cagtcgctcg ccctcggcgt acccggcatc
     3541 gccctgctgc acgtcgaact ggcagcggca gggctaagac cgtggcaacg cgcccatcac
     3601 tggctcgccg ccgctaccag cggcccggta acgacaggcc ccgacagcca ccccttccac
     3661 ggcgcacccg cgctggcgca cgtcctggcg agcgctgccg tccaccagcc cggctcgtac
     3721 gcccgcgcgc tggagggcct cgacacggcg atcgccgccg acgcgcggcg ccgcgtggcc
     3781 gctgcgcacg cccgcatcga cgccggcgag ctaccggctc ttgccgagtt cgacgcgatc
     3841 cgagggctca ccggtatcgg ggcgtacctg ctgcgccgat cgtccggcgg gcaggagctt
     3901 cgcgccgtac tcgagtacct ggtccgtctc acctctcccc tgcaccgcga gggcgagatg
     3961 ctgcccggct ggtggaccgg caacggcccc aacgggcgcc gcgacgaccg attccccggc
     4021 gggcacggca acaccgggat ggcacacggc atcgccgggc ccctggcgct gctggcactt
     4081 gccgccctgc gcggcgtcgt cgtccagggt cagctcgcag cgatcgcaac catctgctcc
     4141 tggctcgacc ggtggcgcag caccaccgat gcaggcccga tctggccgta ctgggtcacc
     4201 aggtcgcagc tgcgcgccgg ccacgcatcg ccgtcgagcg cccagcggcc gtcctggtgc
     4261 tacggcacgg ccggtctggc acgcgcgcaa caactcgccg ctctcgcgac cggtgacctg
     4321 gtccgtcgtg tggctgccga acacgcgctc gcccgcgccc tgaccgaccg acgccagctc
     4381 gccgcgacca ccgatccctc cctgtgccac gggtacgcgg gcctggccca catcgcccac
     4441 cgcgtcgccg ccgacgctct tcccggaaat gcagcccgcc tcaacgcccg ggtcccccac
     4501 ctgctcaacc ggatcgaccc cgcaggtgcc ggccctcggc gtacggctgc tgccctgatc
     4561 cgcgacgtgc cgggccctgg attgctcgaa ggcgccaccg gagtggcact ggcggcgctg
     4621 agctgcagca cctcgtccgt gccggacagc tcctgggact tctgcctact caccgcccgg
     4681 cctacccaac gtgaggatgt ctgatccgat gccgacacct gcatggcggc agtacaccgt
     4741 cgagttctcc acacccgccg cagcggagca gatcgccgcc accgtgctcg ccccagcgct
     4801 ggctgctgcg caggacgctg gcgtcctgca cggctggtgg tacgtccgca agtacccctc
     4861 gtggcggtgg cggtacctcg ccgacgatcc cacctcccac ctcttcgaga atctgctgga
     4921 gacgctcgcc gtcgacgacc gcatcgtcac ctggacccga ggcctctacg agccggagac
     4981 gctggccttc ggcggctcgg ccggcatgca cgtcgcgcac gacctcttcc atcaggacag
     5041 ccgacagctg ctccttggac caggcttcgc cgagtcggcc atgctcggaa gacgcgaact
     5101 cgccatcctt ctgtgcagcg tgctgatgcg cagcgggggc ctggactggt acgagcaggg
     5161 cgacgtctgg accaaggtcg ccgaactacg tcccgcccca gccctacccg ccgaaagcgc
     5221 caccgccgcc gccctgagcc gggcgatcga ccggctcatg tccgtggaca tccgcagcct
     5281 gagccgtccc ggctcggacg ggccgctcgc gggctacggc gcatggctcg acgcgttcga
     5341 ggcagccggt caggccctgg ccgacctggc acggcacggc cgcctacgac gaggcctgcg
     5401 cgcggtcgtc gcccaccacg tcatcttcca cgccaaccga atgggtctgt ccgtacgcga
     5461 ccagtccacc ctcgccgcac tcgcggtcag gaacgtcttc gatcccatca ggaggagcac
     5521 cgtgtccacc tcagcaccca tcccgccggc cactagcgtc gacgagatga ccacgcttcg
     5581 tgaacccgcc gacctgcgat ccgagctcac cgaccggctg cggacagaga acatcatccg
     5641 gacgccgagc gtcaacgccg cgatacggca gacaccacgg catctgttcc tgcccggcgt
     5701 gccgctggag caggcgtacg ccgacgcacc ggtctacacc aaaaccgacg gtcgcggcgc
     5761 ctcgatcagc gccgcctcgc agccccggat cgtggccatg atgctcgaac agctcgacgc
     5821 ccgaccgggc caccggatca tggaagcagg tgccggcacc ggctacaacg cggctctcct
     5881 ggccgccatc gtcggcgaga ccggacgcgt cgtcaccatc gacgtcgatg acgacctcat
     5941 cgacggggcc cggaaacacc tagccgccgc cggcgtcgcc aacgtcgaag tcgtccacgg
     6001 cgacggcgcg ctcggacacc ctgacgccgc accgtacgac cggatcatcg ccaccgtcgg
     6061 tgcctgggaa acgcccacca cgtggctcga gcaaatcgcc ccggacggcc gcctggtcgt
     6121 gccgctccgc ctgcgcggcg ccgcgtcccg ctcgatcgtc tttgagcggt acgaaggtgg
     6181 ctggcgcgac gtcggcagcg agctggccgt cttcatgccg ctgcgcggca ttgcagacga
     6241 tgcgcgacgt ctcgtcaccc tcaccgacga gcaggacgtg accctgcagg tacacaagga
     6301 ccagagcgcc gacgcggccg cactcgctgg tgtgctcgac gccgaacgac acgagacgtg
     6361 gaccggcgtg atcttcccgc ccatggtgcc gttcgagtgg ctggacctat ggctggcctg
     6421 ccgtctcgac aacgccatca tgcggatgaa cgtcaaaccc gaggccatcg agcgcggcac
     6481 cgtctccccc atgttccctt ggggagcgat ggccaccacc cgcggcgccg acctcgcgta
     6541 cctcactctc cggccggcgc cgccggctgc cgacggcggc aagctgtacg aggtgggcgt
     6601 catcggccac ggcccctccg gcgagggcct cgcccagcac gtcgccgacg agatccgcgt
     6661 ctgggacgcc caataccgct cccggaccgt gcacctcgag atccccgact cgcccaccga
     6721 ggcgaacccg tcggctggac ggttcctcct cccccgaccg caccacccca tcaccgtcac
     6781 ctggcagtga cgatgggccc gaccagccac gtcctgcgac gtttccctct tgtggcccga
     6841 ccgcggcccg cagctaaggc tctgaccagc cgcgtcgacg acctgcgcgt cctcgccgaa
     6901 cgcgccgatc atgagggcga cctggctgcg gcgtctgccg tctacaacca ggctgcgctg
     6961 atcgcctcgg actgcggcca gcccgagttg gcccggcact ggtgccacga acacgccgac
     7021 gcgtacctgc acgccctccc cctgggcgcg caggccgcgc ggcatgccct cgagccgctg
     7081 gtcaacctcg cccgcctgca catcagagac ggcaacggcg acgcggctct cgggctcctc
     7141 aacaacctct tccaagctct ctcatcccgc accgacaccc tgatcgacgg ccacccggtg
     7201 ccggcgagca ggctgacccg aaccgacgat gatcatcgcg aggtacggca gtggctgtgg
     7261 ggtgtgcttc tcgcggacgg gtcccgcgca ctgaccggcg ccggccggtg gaaagaggcc
     7321 tacgagcacc tccagcggca caacggcatc ggccaacgga tgctcgacgg gcgccaagtc
     7381 gcggtcattg cccacgccac ctccggcgag attgagcgcg ctcgcgcgat catcaccgag
     7441 acggtccgcg gcgagccgtg ggaggaggcc gtcaccgcat gtctggccta cctgtgcgcc
     7501 agggccggcg gcgagccgga tggtgcctac ctcgacacgc tgctccgcac ctaccgacgg
     7561 ctaacgccca cacccagcct cgccgtcttt cacacccgcc tcggtctcac ggtcctggac
     7621 gcggcgggcg gagtagacca gccgggggta cgaagtctgg ccgcagacct gatcgaccaa
     7681 gctctcgcct atggcgacgc ctacgtggcg cgtgaactcc ttggccaccc cggcatctgt
     7741 gctgtcattg gtgccggacg cgaagctggg ctcgtcgaca tcatgcagga gggaatgatg
     7801 catgcccaac tgccaagcgt tgctcgcaag cggctgctgc agtcgctgac taccagcaga
     7861 gcggtgatca cgaggggttc acttgtgccc gggccgaggc tcgaaccggg tggcgtgggc
     7921 cggcaaatcg gcggcgtagc caccgaggaa gccctgcccg gctgacgaac atccccacgt
     7981 cgctggcggc attggctgcg tatccgcgaa tctcgtaccc gccgagcacg gtccggtggc
     8041 gtaaggccag gcgcagccgg gcgagggact cgacgtcgat cgaaccgtgc aggcgggcgt
     8101 gatgccccaa tgactgcaag gcgttggccc gcgtctgcgc cctcatcgag cccagcccgc
     8161 gcttgatccg cggcagcacg acgtcagggt cgtgatggtt aagcgtcaaa gcgagaagag
     8221 caacgcccgc gtactcatcc tcttggccga ggagttcgtc gaccagattc ggctgttgga
     8281 ccacgctcat ccagcttccg atcaaccaag gtgaatcggt cgtcatcgta ggatgatgtc
     8341 acgctggctg gacgccaccg accgggcgat gaggacccgg gcgtcacccg cccgcggccg
     8401 gtcaggaggt caaacaacct gcgtctgctc ccgtcggtcc atagcctcat aggcttctcg
     8461 cagccgagcg gtcacgtcga ctgcgccatc gatgccagca cgggtctgac tcacggttat
     8521 ccggtgaccg gcgacccatg agctcgctcg ggcggctgtc ttgacctggc cggctggcac
     8581 ctcgacggct tgctcgatgt cgtaggtgac gctgttcagg atgacaaaca cgcaggcatc
     8641 gaagctccaa ctacggaacg gcgagtagac ctgggtacgt cggctgccgg gctcgatgac
     8701 gcgacacttg acctgcagca aggtgccgcc tgccgctcga acgtcccaac tcttctccga
     8761 ctgtggcgcg agctcgcctc gataggcctt cgcaacgacg gattcggcca ggtcaccggc
     8821 aggggcgttg cgcgacctga caacgccgcg ctggatcagc tcattgagga tccgagagta
     8881 gagcccaagc agttgccgga tgctcatcga tcggagatca ggatcggtag ctggctgcac
     8941 ggcgtgacgg tacacccagc cgcgtataag cactcctcgc gaacccgctc tgcagcaagt
     9001 gagaaccccg ggccaggccg cggtcactaa acttcccatc acctcaatta gggcgtaggt
     9061 agtttctttc cttcttccac tggcccaaat ttggcaacag ctcccggaat acgccacctt
     9121 cggtggtgaa cgaatcaacc agataccact cgttcgcctg ccagatcgac gggattttga
     9181 ccatccgtct cagagggtgg ttcgggaggc tttgtctgat gtgggtttga cggttcgtta
     9241 gcggccctgg tgggggctac gcggacgtcg ccggcggggc gagggtgaac agtccggcct
     9301 cgggttcggt gagga
//
