LOCUS       NZ_CP072563            10852 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Bacillus sp. LJBV19 chromosome, complete genome.
ACCESSION   NZ_CP072563
VERSION     NZ_CP072563.1
KEYWORDS    RefSeq.
SOURCE      Bacillus sp. LJBV19
  ORGANISM  Bacillus sp. LJBV19
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 3973013)
  AUTHORS   Lu,J., Wang,B., Fu,P. and Peng,H.
  TITLE     Complete genome sequence of Bacillus velezensis LJBV19, a biological
            control agent and plant growth promoting bacterium isolated from
            soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3973013)
  AUTHORS   Lu,J., Wang,B., Fu,P. and Peng,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) School of Agriculture and Biology, Shanghai
            Jiao Tong University, Jiangchuanlu, Shanghai 200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V4; CANU v. v1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 318.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017797845.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 04:46:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,800
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 39 of 66
            Pseudo Genes (incomplete)         :: 42 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 23 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:43:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 392746
            Orig. end                         :: 403598
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP072563.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5852
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            40..993
                     /locus_tag="J6600_RS01955"
                     /old_locus_tag="J6600_01955"
     CDS             40..993
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016937258.1"
                     /locus_tag="J6600_RS01955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01955"
                     /product="ABC transporter permease"
                     /protein_id="WP_015239156.1"
                     /transl_table=11
                     /translation="MKLRYLLLLLAVLACTSIFIGVEDLSPLDIFHLNKEQAGTLFDSR
                     LPRLVSIIIAGMSLSICGLIMQQISRNKFVSPTTAGTMDWARLGILISLMLFTSASPLV
                     KMSIAFVFALAGNFLFMKILERIKFNDAIFIPLVGLMLGNIVSSIATFIAYKYDLIQNL
                     SSWLQGDFSLVVKGRYELLYLSIPLVIIAYVYADKFTVAGMGESFSVNLGLKYKRVVNI
                     GLIIVSLIASLVILTVGMLPFLGLIIPNMVSIYKGDNLRSSLPHTALLGAVFVLFCDIL
                     GRVIIFPYEISIGLMVGVIGSGIFLYMLLRRKAYES"
     gene            983..1930
                     /locus_tag="J6600_RS01960"
                     /old_locus_tag="J6600_01960"
     CDS             983..1930
                     /codon_start=1
                     /function="ABC-type enterochelin transport system,permease
                     component"
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388263.1"
                     /locus_tag="J6600_RS01960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01960"
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="WP_014416905.1"
                     /transl_table=11
                     /translation="MSHNKKITLLAALAMICIGFFLFYGLGNWEYSLPRRCFKVLAMML
                     TGGAIAFSTVIFQTITNNRILTPGILGLDSLYMLIQTVIVFLFGSMNIVMMNKNLNFVI
                     SVGLMVLLSLVLYQLMFKGEGRNVFFLLLVGVVFGTLFNSLSSFMQMLIDPNEFQVVQD
                     KMFASFNNINTDLLWFAGAVFLLTGIYVWRFTRFFDVLSLGREHAVNLGIDYDKLVKQM
                     LIVVAILVSVSTALVGPIMFLGLLVVNLAREFLKTYRHTYLIAGSVLISIVALVGGQFV
                     VEKVFTFSTTLSVIINFAGGIYFIYLLLKENKSW"
     gene            1924..2682
                     /locus_tag="J6600_RS01965"
                     /old_locus_tag="J6600_01965"
     CDS             1924..2682
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388264.1"
                     /locus_tag="J6600_RS01965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01965"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_063636991.1"
                     /transl_table=11
                     /translation="MVEVKNVSKQYGGKVVLEKTSLSIQKGRLTSFIGPNGAGKSTLLS
                     IMSRLIKTDSGEITVDGQEIGACKSNDLAKKISILKQTNQINIRLTIKDLVSFGRFPYS
                     QGKLTDEDWVHINQALSYMKLDELKDKYLDQLSGGQCQRAFIAMIIAQDTDYIFLDEPL
                     NNLDMKHSVEMMKLLKRLVEDFGKTVVVVIHDINFASVYSDHIVALKNGRIVKEGPAEE
                     IIETSVLEDIYDMTIPIQEIDSQRIGVYFS"
     gene            2704..3648
                     /locus_tag="J6600_RS01970"
                     /old_locus_tag="J6600_01970"
     CDS             2704..3648
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234484.1"
                     /locus_tag="J6600_RS01970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01970"
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_012116805.1"
                     /transl_table=11
                     /translation="MKKFALLFIALVTIFVVSACGNGDKTSGKSKETITIKDDLNKEGV
                     KVPKNPKKVVVFNFGMLDTMDELGLQDHVAGLPKQNLPKYLSSYKSDKYANTGGLKEPD
                     FEKVADIDPDLIIIAHRQSDSYKEFSKIAPTIYLDDDYTNYVDRFKHNTEVIGKIFNKE
                     NEVKDKLAKIDKSIADLKKKTSSTDKNGLVVMANDGKISAFGSKSRYGFIHDVFGVKPA
                     DKNIEASLHGQSISYEYIAKTNPDYMFVVDRGTAIGTKSSTKQVVENDYVKSVKAVKNN
                     HVVYLDSATWYLSGGGLESMAQMVKEVKDGIEK"
     misc_feature    4178..4192
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.4"
                     /tool="antismash"
     misc_feature    4500..4514
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    4501..4515
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.93"
                     /tool="antismash"
     misc_feature    complement(4857..4870)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.23"
                     /tool="antismash"
     gene            5001..5852
                     /locus_tag="J6600_RS01975"
                     /old_locus_tag="J6600_01975"
     CDS             5001..5852
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="J6600_RS01975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01975"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_209387881.1"
                     /sec_met_domain="DUF692 (E-value: 5.8e-28, bitscore: 95.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYPNLGVGMLVHNSEVYMDYLERLGDVSEVYELLLIQYLNEPDE
                     LNKMVDRLGKPIIAHTTRFSFGTDTPMDSKLLNSMNDMMLRCKAKWCGDHLCFTGVPGL
                     AAGGLLPPIGNEESIDIFVRNILEAKKVINTPLLIENVVEYFQAGTITHAEILKEVVER
                     ADIGIVLSMENISSSKMYSDCDYYEFIDTIPSDRIVQIHCTIGNSKQQKEMKSDIFGTV
                     YDKQLMQYKALEYLSKKSGVQPKAFIWELETETDSHPEVEELRERMNWAKSLFFKNKEV
                     VS"
     gene            5852..6649
                     /locus_tag="J6600_RS01980"
                     /old_locus_tag="J6600_01980"
     CDS             5852..6649
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="J6600_RS01980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="J6600_01980"
                     /product="hypothetical protein"
                     /protein_id="WP_209387882.1"
                     /transl_table=11
                     /translation="MSTLQLQTFLTCYIRNNSLRERMKSQSTENILYEYDLSKEELKLI
                     ETLDFTQLNRTADHLEEERKGKRCADFSEFTSVLQQANLFDSFFSSFTQSYTDGFLTSE
                     IEAERFLEHAHKYISDNSLPDILADLATYSYQVFRISQVEIQDVYNKNKLNWNKPLFLK
                     RPFSKKTFSYNLMEIINNENIKKIEDIETIQKKETTYLFQKDYSNLISSNIFEVDDSVF
                     FRLVEQNLTGYEILNRANSINEKGKWRDLISELYDLDIVGTTD"
     gene            6905..8701
                     /locus_tag="J6600_RS01985"
                     /old_locus_tag="J6600_01985"
     CDS             6905..8701
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /locus_tag="J6600_RS01985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01985"
                     /product="M3 family oligoendopeptidase"
                     /protein_id="WP_209387883.1"
                     /transl_table=11
                     /translation="MTNQLNKYSLTWDLDAFFLGGSLSEDFKIYMNHIKTDIKKFELLV
                     SSSELKESRDLTIWRNILDIWQDLSCRLGEAEAYISCLIAQDMRDKEARNLKMNFSVIE
                     SAFNAALEKLDQLFLEMKKSDWEKMMNDDYFKQVSFPLKERREKIKELSSLDKENLIKE
                     LAIDGYHAWGDLYSNIISKITIPYTINGSSKLLSVGQAMNILDSSDRSIRQQIFHLLND
                     IWEREEDLIGSILNHLCGFRLNVYNQRGWSNPVKESLINNRISEETLLTMWDVINKNKQ
                     GLIQFLERKAKIIGVDKLAWYDVFAPLGKTKSQMSYEDAVDTIVKKLGEFDMDLSVFAE
                     KVFQEKWIEAEDRPGKRSGAFCIYFSDSAQSRIFTTFSGSSSIVSVLAHEIGHAYHQYT
                     MRDLPKLVQNCGLCLSETASMLTELIVTDSAICETVSKEQKILLLEEKIRQAVSCLLGI
                     QARFLFETRLNNERKKGIVSAKRLKELTVQAQQDAFSDCLSEYHPWLWASRLHYYITEV
                     PFYNYPYTFGYLFSIGIYKRYSENKINFSNQFIELLKSTGSMTAEELALKYLGVDLTGT
                     EFWQDAIELIKDDINNFLELTE"
     gene            8750..10003
                     /locus_tag="J6600_RS01990"
                     /old_locus_tag="J6600_01990"
     CDS             8750..10003
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /locus_tag="J6600_RS01990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01990"
                     /product="MFS transporter"
                     /protein_id="WP_209387884.1"
                     /transl_table=11
                     /translation="MDEKGLFKKNLNFRYIWFAQSGSTLGDWFNQVAITQTVLMLTNSP
                     AAVGMLLLCRSLPVVFLGPILSPLVDKWPKKVIMIATDILRALLVLSFIGAIVYNNVYV
                     LYINSFLFGVISVLFNPTKQALLPLIVNRKDLPEANAFMSSTDAIVNIIGAVLGGIVST
                     VFSPITCFLVNSLSFLWSAYYVFKIKYQENKKSDIQNQPYIQLLKEGLLESNQNILIRY
                     VILIGLTWGLVGGGYYILIPFLGNNIYHLGAVGIGALYAIDGLGILLGTYIVKKIIKNN
                     FKRALFSFGISYSVQSMLFVLLTHSTVFYLGSLFLFLMRVCGGVIIPLSNYFVQMGTTD
                     EIRGRVFSLYNSTYMGVMQISYFISGYAYEKYSVPIVGAIGGMVSMLCGLIWLLQVMKG
                     KFAGYRENNRTSQKFIDY"
     gene            10035..10715
                     /locus_tag="J6600_RS01995"
                     /old_locus_tag="J6600_01995"
     CDS             10035..10715
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /locus_tag="J6600_RS01995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J6600_01995"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_209387885.1"
                     /transl_table=11
                     /translation="MKPDYVDNLEEMEKFFDEKAKDWDEYAKHDPYNHYFKAISSHVSE
                     TKEKIRILELGCGTGLTLHSILDKAPNAIITCIDLSENMLEVLSKKFTEYSSQIQIRHG
                     SFFEFPFGKEQFNYIISSQTFHHFTPNTKKALYEKIYLSLERGGKYIEGDFLVSIEEEK
                     ESLNEYYKYVAPLEEKEEGKYHFNIPLTLETQKQLLSDSGFKWENDIYYEDETELAVYT
                     GLKE"
ORIGIN
        1 aatcagtcat accgtttgtg atgggtgcag ggaggcagca tgaagctacg ttacttactt
       61 ttgctactgg cggttttggc gtgtacttct atatttatcg gtgtagaaga tttgtcgccg
      121 ctggatattt ttcatttaaa taaagaacag gcaggtacat tgtttgacag ccggctgccg
      181 cgtttggtga gcattatcat cgccggcatg agcctgagca tctgcggatt aatcatgcaa
      241 cagatcagcc ggaataaatt tgtatcgccg acgactgcgg gaacgatgga ctgggcaagg
      301 ctcggaattt tgatttcatt aatgcttttt acgtcagcga gtccgctggt gaaaatgtct
      361 atcgctttcg ttttcgcgtt ggcaggaaat ttccttttca tgaaaatcct cgagagaatc
      421 aaattcaatg atgcgatttt tattcctctc gtcggtttaa tgcttgggaa catcgtcagc
      481 tcaatcgcca cgtttatcgc atataaatac gatctgatcc aaaacctgtc atcttggctg
      541 cagggagatt tttcactggt cgtcaaaggc cggtatgagc ttttatatct gagtatcccg
      601 ctggtgatca tcgcttatgt gtatgcggat aaatttacgg tggcgggaat gggagagagc
      661 ttttccgtca atctcggcct gaaatacaaa cgcgtcgtca atatcggtct gattatcgtg
      721 tcgctcatcg cttcactggt gatcctgacc gtcgggatgc tgccgtttct cggtttgatc
      781 attcccaata tggtgtcgat ttacaaaggg gacaacctga ggagcagcct gccccacacc
      841 gctctgctgg gcgctgtttt tgtgctgttt tgcgacattt tgggaagggt gatcatcttc
      901 ccgtacgaaa tttccatcgg cctgatggtc ggtgttatcg gcagcggcat cttcctgtat
      961 atgctgttaa ggagaaaagc atatgagtca taataaaaaa attacgctgc tggcggcgct
     1021 cgccatgata tgtatcggat tcttcctgtt ctacggactg ggcaattggg aatactccct
     1081 gccgagacga tgcttcaaag tgcttgccat gatgcttacc ggaggagcga tcgcgttttc
     1141 aacggtcatc ttccagacga ttacaaataa ccgcatactg acaccgggca ttttaggcct
     1201 cgattccctg tatatgctca ttcagactgt catcgtgttc ctgttcggtt caatgaatat
     1261 cgtcatgatg aacaaaaatc ttaatttcgt catctccgtc gggctgatgg tgctcttgtc
     1321 actcgttttg tatcagctca tgtttaaagg cgaggggcgg aacgtgtttt tccttctgct
     1381 tgtcggcgtc gtgttcggca cgctgttcaa cagtctgtca tcctttatgc agatgctgat
     1441 tgatccgaat gagttccagg ttgttcagga caaaatgttt gccagcttta acaatatcaa
     1501 caccgatctt ttatggtttg ccggtgctgt ctttcttctg acgggcattt atgtctggcg
     1561 gttcacgcgg ttttttgacg tgctgtcgct cggcagggag cacgccgtca acctggggat
     1621 tgattacgat aagcttgtga aacagatgct cattgtcgtc gccattcttg tgtctgtctc
     1681 tacggctctt gtggggccga ttatgttttt aggactgctt gtcgtcaatc tggcgagaga
     1741 gtttttaaaa acctacaggc acacgtattt aatcgcgggt tctgtcctga tcagcatcgt
     1801 cgcgctcgtc gggggccagt tcgtcgtgga gaaagtgttc acgttttcca cgacgctgag
     1861 cgtcatcatt aattttgccg gcgggattta ttttatctat ctgctgttaa aggagaataa
     1921 atcatggtag aggtcaaaaa tgtaagcaaa caatacggag ggaaagtcgt ccttgagaaa
     1981 acgtctctat ccattcaaaa gggcaggctc acctcattta tcggtccgaa cggcgcggga
     2041 aaaagcacgc tgctgtctat catgagccgg ctgattaaga cggattccgg ggaaatcacg
     2101 gtcgacgggc aagagatcgg ggcctgcaaa agcaacgatc tggcgaaaaa aatcagcatc
     2161 ttgaagcaga cgaaccaaat caatatcaga ctgacaatta aagacctggt cagcttcggc
     2221 agatttccgt attcccaagg gaaactgacg gatgaagatt gggtgcatat caaccaggcc
     2281 ctcagttata tgaagctgga tgaattaaag gataaatacc tcgatcagct cagcggaggc
     2341 cagtgccaga gggcatttat cgcgatgatc atcgctcagg atacggacta tatttttctc
     2401 gacgagccgc tgaacaattt agatatgaag cattccgttg aaatgatgaa gctgttgaag
     2461 cggcttgtgg aagacttcgg aaaaacggtc gtcgtagtga ttcacgatat caacttcgca
     2521 tccgtatatt cagaccatat cgtcgctctg aaaaacggcc ggatcgtcaa agaagggccg
     2581 gcggaggaga tcattgaaac ctccgtgctt gaagatattt atgatatgac catcccgatt
     2641 caagagattg acagtcagcg gatcggtgtg tacttttctt aaaaaccaga tgaggtgaaa
     2701 atcatgaaaa aattcgcatt gctattcatt gcgctagtta cgatttttgt tgtttctgca
     2761 tgcggaaacg gggacaaaac atcaggcaaa tcaaaagaaa caatcacaat caaagatgac
     2821 ctgaacaaag aaggagtcaa agtgccgaag aatccgaaaa aagtcgtcgt attcaacttc
     2881 ggaatgctgg acacgatgga tgaactcggt ttacaggatc acgtggcggg acttccgaaa
     2941 caaaaccttc caaaatactt atcttcatat aaaagtgata aatacgccaa taccggcggt
     3001 ttaaaagaac cggattttga aaaagtcgcg gatattgatc cggatttaat cattatcgcc
     3061 cacagacaat ctgattctta taaagagttc tcaaaaatcg cgccgacgat ttatcttgat
     3121 gatgactata caaactacgt tgacagattc aagcacaaca ccgaagtgat cggcaaaatc
     3181 tttaacaaag aaaatgaagt gaaagataag cttgcgaaaa ttgacaaatc cattgccgat
     3241 ctgaagaaaa agacaagcag cacggataaa aacgggttag tggtgatggc gaacgacggc
     3301 aaaatcagcg cgttcggatc taaatcaaga tacggcttca ttcatgatgt gttcggcgtc
     3361 aaaccggctg acaaaaacat tgaagcctca ctgcacggac aaagcatttc ctatgaatat
     3421 atcgccaaaa caaaccctga ttacatgttt gtcgttgacc gcggaacagc gatcggcaca
     3481 aaatcttcaa caaaacaagt ggttgagaat gattatgtaa aatcggtgaa agccgtgaaa
     3541 aacaaccatg ttgtctacct tgattcagct acatggtatt tatcaggagg cggcctggaa
     3601 tccatggcgc aaatggtgaa agaagtaaaa gacggaatcg agaagtaaat taaattatta
     3661 tctttttaaa aggcttgagg ctgtcggact gccctgatct gagagcatct ttaaacagcc
     3721 agctgccgac agaagatcgg cgactggctg ttttgttgta gaattaatat tcaatagtta
     3781 ctctgcaatg actgtcgtaa tgttgagata gaatattcaa acttactgat ttactggtta
     3841 atgaaattat ataaaagaat cagttagagg ctattggaaa atgctaactt cgactgtttt
     3901 ctgatactct tgagttggaa tttttacatg tgtcaggaat taaccttcct acagaatagg
     3961 cagcgaccgt taaaataagg agtggtgtca cagttagttc aatctttatg aaaagaaacc
     4021 tgtcttgtta cagtcctaat cagcaggaat ttaaataata tcgtaggtat ctcattcctc
     4081 tgtctttgtg aacactacaa aaagtcttaa ttaagaggca acattgaaac tcacgtgttt
     4141 agctcaatgt tccttttttt ggctgcctgt ttgttggttt tttctgataa tcatgcttaa
     4201 tcaagagata ccaagatccc gatacatgtt gttggcatca attcagttcc tcttactaat
     4261 tctgcgactg tagacggtac tgttccctct ccccatactt catacttttt tagaacttca
     4321 gtttaacctt tccgaagtta tttcagattt cagttccttt gtttgtaaat tatccaaaac
     4381 gtatactagc ttatccatct gataagagtg gataggagcc cacggatgct taattatttc
     4441 atccgtgttt tgaaattttc atgcttgaat tttaatttga tttttaatct tttaacaggc
     4501 gtgctgatcg gccattcttg ttgaatttct ttaaagcatc attgatcatt ctaatttcat
     4561 gaaaaatgct accaaaattg ttttaaattc tccgttttca actagacggc attttatttg
     4621 aggaaagctt caggtgcata atagagaaga tttttcttag ttatggaaaa aataaatgtt
     4681 attaaaggct aaaggagaga aaagagaaag atcagacttt ttaagttcaa gaataattat
     4741 taatagtcct gttaaaagga taagacgaga ccttcagaag aaagcgttaa attaaacata
     4801 aaaggttttt ttctctcatt aactctgcat atacaaaaaa atgtagaata atgtagtttt
     4861 ttgtaaaaat atgaaaaaat ttgtagtaat aaaaatgttt aaaagtgtta aaatgtgtta
     4921 tataatccaa ttaaagggtt ttaatggatt tttagatttg tgaaatttat ttacaggaag
     4981 tattgggagg cctgtttaga atgaattacc cgaatttagg agtaggtatg cttgtccata
     5041 attctgaagt ttacatggat tatcttgaaa gattggggga tgtatccgaa gtctatgaat
     5101 tgttacttat tcaatactta aatgaaccag atgagttaaa caaaatggtt gatcgactag
     5161 ggaagcctat tattgctcat actacaaggt tttctttcgg aacagataca cctatggata
     5221 gtaagctgtt aaatagtatg aatgatatga tgctcagatg taaagcaaaa tggtgcgggg
     5281 atcacttatg ttttacaggt gttccaggct tggcagctgg tggtttactt cctcctattg
     5341 ggaatgagga gtctattgat atctttgttc gtaatattct tgaagctaaa aaggtaatca
     5401 atactccgct cctgattgag aatgttgtag agtattttca ggcaggaaca ataacacatg
     5461 cggagatcct gaaagaagta gttgagaggg cggatattgg aatagtgctc agtatggaga
     5521 atatcagcag ttcaaaaatg tattccgatt gtgattatta cgagtttatt gacactattc
     5581 cttctgaccg gattgttcaa attcactgta ccataggtaa cagtaagcaa caaaaagaga
     5641 tgaaatctga tatatttggc acggtttatg ataaacagtt gatgcagtat aaggctttgg
     5701 agtatctatc caaaaaaagc ggtgtacaac caaaagcgtt tatatgggag ttagaaactg
     5761 aaactgattc tcatccagaa gttgaagaat tgcgtgaacg gatgaattgg gctaaatcat
     5821 tgtttttcaa gaacaaagag gtggtctcgt aatgagtaca ctacaattgc aaacctttct
     5881 aacttgttat atcaggaata attctttacg ggaaagaatg aaatcacaat caactgagaa
     5941 tattctttat gaatatgatc tatcaaaaga agagttaaaa ttaatagaga ccctagactt
     6001 tactcaactt aatcggactg ctgatcattt ggaagaggaa cgaaaaggaa agagatgtgc
     6061 tgatttttcg gagtttactt cagtcttgca gcaagcaaat ctatttgatt cattcttcag
     6121 ctcatttacg cagtcttata ccgatgggtt tctcacatca gaaatagagg cagaacgctt
     6181 tttagagcat gctcataagt atattagtga taatagttta cccgatattc ttgcagatct
     6241 tgccacatat tcatatcaag tatttagaat atctcaagtt gagatacagg atgtatataa
     6301 taaaaataaa ctcaactgga ataagcctct ctttcttaaa cgaccattta gtaaaaaaac
     6361 tttttcttat aacttaatgg aaattattaa taacgaaaat attaaaaaaa tagaagatat
     6421 agaaactatt caaaaaaaag agacaacata cttatttcaa aaagattact caaatttgat
     6481 tagttcaaat attttcgaag tagatgattc tgttttcttc agactagtgg agcagaactt
     6541 aactggttac gaaattctga atagggcaaa ttccattaat gagaagggta aatggagaga
     6601 tttaatttct gagttatatg atttggacat agtcggaact acggattgaa agtattactt
     6661 gaaagggggt gaaatcatga aaaaagtaca agtgcgtaaa aatgacggaa aagtcgaaaa
     6721 aaacagcaat aatggtaagc aataatctgt aataacaaag aaatatgcgg gggctgtccc
     6781 ccgcatattt tgtttagaat aacattatga aaaaaataat ggtatatttc aagagtttta
     6841 agtattttac tgcttatttt agtagttaat cgtgggccac aaaggtaagt gaaagggtag
     6901 tgctatgact aatcaattaa ataagtatag cttaacatgg gatttggatg ctttcttctt
     6961 aggaggaagc ttatctgagg attttaaaat ctatatgaat catattaaaa ctgatataaa
     7021 gaaatttgaa cttctagtaa gttcttctga actaaaagaa agtagagatc tcacaatttg
     7081 gagaaatatt cttgatatct ggcaagattt atcatgtcgt ttaggagaag cagaggctta
     7141 tattagttgt ttgattgctc aggatatgag agataaagaa gcccgaaatc taaaaatgaa
     7201 tttttcagta attgaatcag ctttcaatgc agcactagaa aagctagatc aattatttct
     7261 ggaaatgaaa aagtctgatt gggaaaaaat gatgaatgat gattatttta agcaagtttc
     7321 ttttcctttg aaggagagaa gagaaaaaat aaaagagctt tcatctttag ataaagagaa
     7381 tctaatcaag gaactggcta ttgacggcta tcatgcttgg ggagatttgt atagcaatat
     7441 aattagtaaa ataacaatac cttatacgat aaacggaagc agcaagcttc tatctgttgg
     7501 acaggcaatg aatatattag acagctctga tcgatccatt cgccaacaaa tatttcattt
     7561 attaaatgat atttgggaga gagaggagga tcttatagga tctattctaa atcatttatg
     7621 tggattccgt cttaatgtat ataatcagcg gggatggtca aatcctgtta aagaatcatt
     7681 gattaataat agaatttctg aggaaaccct attaacaatg tgggatgtaa ttaacaaaaa
     7741 taaacaaggt ctaattcaat ttttagaacg caaggcgaaa attataggag tggacaagtt
     7801 ggcttggtat gacgtatttg caccacttgg taaaaccaaa agccaaatga gttatgaaga
     7861 tgctgtagac accatagtta aaaaattagg ggaattcgac atggatttat cagtttttgc
     7921 ggaaaaagtg tttcaagaaa aatggataga ggctgaggat cgtcccggaa aacgatctgg
     7981 agcattttgt atatattttt ctgacagtgc tcaatcacgt attttcacga cgttttctgg
     8041 tagttcatcg attgttagtg tgcttgccca tgaaataggt catgcatacc accaatatac
     8101 tatgcgtgac ttacctaaat tggtgcaaaa ctgtggatta tgcttatcag aaacagcttc
     8161 aatgctgact gaacttattg ttacagactc cgcaatttgt gaaactgtga gtaaggaaca
     8221 aaaaatctta cttttagaag aaaaaataag gcaggcagta tcttgcttgc ttggtataca
     8281 agctcgcttt ctttttgaga caagacttaa taacgagcgg aaaaaaggga tagtcagtgc
     8341 aaaacggctc aaagagctaa ctgttcaggc acagcaggat gcctttagtg attgtctctc
     8401 tgaataccac ccttggctgt gggcttctag acttcattat tatattacag aagtgccttt
     8461 ttataattac ccatatacat ttggatattt attcagtatt gggatttata agagatattc
     8521 agagaataaa ataaattttt cgaatcaatt tattgaattg ttgaagagta caggaagtat
     8581 gactgcggaa gagttggctc taaaatattt aggcgttgat ctaacaggca ctgagttttg
     8641 gcaggatgca atcgaattaa tcaaggatga tatcaataac tttctagaat taacagaata
     8701 gaaaaacaat gtaataattt cgaggttaac gttaaaggtg tggtaataag tggatgaaaa
     8761 aggcttattt aaaaagaatt taaatttccg atatatttgg tttgcgcaat cgggcagtac
     8821 tttaggtgac tggttcaatc aggtagctat cacacaaaca gtgctaatgt taactaattc
     8881 tcccgcagca gtaggtatgt tattgctttg tcgttcttta ccagttgttt ttttaggacc
     8941 tattttaagt ccactcgttg acaaatggcc aaaaaaagta atcatgattg ctacggatat
     9001 tctcagagct ttgcttgttt taagttttat aggggctatt gtctataata acgtatatgt
     9061 gttatatatt aattcatttt tgtttggtgt aattagtgtc ttatttaatc cgacaaaaca
     9121 agcgttgttg ccactaattg ttaacagaaa ggatttgcca gaagcaaatg catttatgtc
     9181 tagtacagat gcaatagtta atatcattgg ggctgtttta ggtggtattg tatccactgt
     9241 atttagtcca ataacgtgtt ttctggttaa ttccttgtca tttctttggt cggcttatta
     9301 cgtttttaaa ataaaatatc aagaaaataa gaaatctgat attcaaaatc agccctatat
     9361 acaactttta aaagaaggat tattggaaag caaccagaac attttgatcc gttatgtaat
     9421 cttaattgga ttaacatggg gattggttgg tggaggatat tatattctaa ttcctttttt
     9481 aggtaataac atttaccatt taggtgctgt aggtataggc gctttatatg ctatagacgg
     9541 actgggtatt ttgttaggta cttatattgt aaagaaaata attaaaaata attttaaacg
     9601 tgcactattt tcttttggaa tatcttactc agttcaatcc atgctgtttg tgcttttaac
     9661 acactccaca gtgttctact taggaagtct atttttattt ttaatgagag tatgtggggg
     9721 ggtcattatt cctctaagta attattttgt gcaaatgggg acaaccgatg agatcagggg
     9781 aagggtcttt tcattatata attcaaccta tatgggtgta atgcaaattt catactttat
     9841 tagtggttat gcttatgaga aatatagtgt gcctattgtc ggagcaatag gaggcatggt
     9901 atccatgtta tgcggattaa tatggttatt acaagtaatg aaggggaaat ttgcaggata
     9961 ccgggaaaat aataggacta gccaaaaatt tattgattat taaattaact cgaactaatt
    10021 taggaggggt aaatttgaaa cctgattatg ttgataatct cgaggaaatg gaaaagttct
    10081 ttgatgaaaa agcgaaagat tgggatgaat acgcgaaaca cgatccttat aatcattatt
    10141 ttaaagctat ttcttctcat gtaagtgaaa caaaagaaaa gattagaata ttagaacttg
    10201 gttgcggtac cgggttaaca ttacactcca ttttagataa agccccaaat gcaataataa
    10261 cttgtattga tttatcagag aatatgctgg aggtgctgag taaaaaattt accgagtata
    10321 gctcacaaat tcaaataaga catggatcgt tttttgaatt cccatttggt aaagaacaat
    10381 ttaactatat aatttcttct caaacatttc accactttac acctaacaca aaaaaagcat
    10441 tatatgaaaa aatctactta tctttagaaa ggggcggaaa gtatattgaa ggggactttt
    10501 tagtatcaat agaagaggag aaagagtcac ttaacgagta ctataagtat gtagcacctc
    10561 ttgaagaaaa ggaagaggga aaatatcatt tcaacatacc gttaacctta gagactcaaa
    10621 agcaattact atcggattcc ggatttaaat gggaaaacga tatatattat gaagacgaaa
    10681 ctgaattagc tgtatatacg ggattaaagg aatagtataa atgtcataga cataaaataa
    10741 agaggtgaaa tttatgtgtg ggatttttgg ttttttaaat aactttcatg gtgataaaca
    10801 agatctaata acaaaacagc ataatcttat taagcatcga ggtccggacg at
//
