LOCUS       NZ_CP040419            10297 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3069 chromosome, complete genome.
ACCESSION   NZ_CP040419
VERSION     NZ_CP040419.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 372x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655825.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:32:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,913
            CDSs (total)                      :: 1,844
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 6 of 44
            Pseudo Genes (multiple problems)  :: 5 of 44
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:54:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 816310
            Orig. end                         :: 826607
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040419.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    735..748
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.5"
                     /tool="antismash"
     gene            975..1574
                     /locus_tag="FE326_RS04025"
                     /old_locus_tag="FE326_03950"
     CDS             975..1574
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636437.1"
                     /locus_tag="FE326_RS04025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03950"
                     /product="response regulator transcription factor"
                     /protein_id="WP_112779370.1"
                     /transl_table=11
                     /translation="MKILLIDDHKLFSQSLALVLDQTTSDVQVDMINSEGELPDDLSEL
                     TVYDVLVLDINLDKGFSEDGFELAERVRAVAVDLPILMLTGFDLPVYEYQAHKLELSGF
                     VNKNIGTEDLLSLLKHVKDGGRHFTTENWFIDELTPRERELLGAIATGKKRKMIAEELY
                     ISERTLTNHMQSIMDKLEVNSTIEAIQKARELGYLK"
     gene            1702..1884
                     /locus_tag="FE326_RS04030"
                     /old_locus_tag="FE326_03955"
     CDS             1702..1884
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS04030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_03955"
                     /product="hypothetical protein"
                     /protein_id="WP_111974584.1"
                     /transl_table=11
                     /translation="MMKYAPYSLLALAGILVYLTVNRYITMHSINVVPPIGAILCVIFF
                     FVWRNHLKQQGYYDD"
     gene            complement(1920..2291)
                     /locus_tag="FE326_RS04035"
     CDS             complement(1920..2291)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS04035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_143333344.1"
                     /transl_table=11
                     /translation="MDNSLPANKSSQLYLIDNRMLDSHLKQLHQHYSAKLELFHSIVAN
                     HPINQHLPYLPPRALKTHLPLPNHINLRDLIKTCQRHHLPNSLDSDHFPLLLELSTIPL
                     DTLEEVTQQLLDIILSLLQ"
     gene            2407..2751
                     /locus_tag="FE326_RS04040"
                     /old_locus_tag="FE326_03960"
     CDS             2407..2751
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS04040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_03960"
                     /product="hypothetical protein"
                     /protein_id="WP_111951603.1"
                     /transl_table=11
                     /translation="MKVECTAVERLIQRGVNIKHKQLPKICFWLGVVVFTVAVLLPTDL
                     FQSITWIEKILGELKPVGLATIFLLPIIGSVGVVGSIMNKSVLYGSLNVALILSFPLMI
                     LVSNIVQALF"
     gene            2752..2994
                     /locus_tag="FE326_RS04045"
                     /old_locus_tag="FE326_03965"
     CDS             2752..2994
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636435.1"
                     /locus_tag="FE326_RS04045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03965"
                     /product="DUF6290 family protein"
                     /protein_id="WP_208954650.1"
                     /transl_table=11
                     /translation="MIKNKLSIVSVRLNKEEEKAINSYATLTGQPVSQLLKQALLREIE
                     DKVDYTIGVEALEEHEKDLQTFTIDEVAGELGIEL"
     gene            complement(3111..3740)
                     /locus_tag="FE326_RS04050"
                     /old_locus_tag="FE326_03970"
     CDS             complement(3111..3740)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152256.1"
                     /locus_tag="FE326_RS04050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03970"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_236654080.1"
                     /transl_table=11
                     /translation="MINQAWGQFSRGNIPKAKQLVDDYINEQATTDYNALNLLGYIYLE
                     DGKIDKAESTYQQWLQLSRKNHDIEQEHIALHQLAMVYRMREDYGQALELIQQEARIIE
                     QHFPNNLLKQSVNNYEQGYLRMKLQQLNEAVSFMAQSLTQAEQTDDLIAQACAQRGMGE
                     ICVAQHKPSLARKYFKRAIQLFEQGGDTIGAQEVQLLMSQLLTDKT"
     misc_feature    3810..3823
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.59"
                     /tool="antismash"
     misc_feature    complement(3832..3848)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.87"
                     /tool="antismash"
     misc_feature    3837..3850
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     misc_feature    complement(3838..3851)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.28"
                     /tool="antismash"
     gene            3877..4761
                     /locus_tag="FE326_RS04055"
                     /old_locus_tag="FE326_03975"
     CDS             3877..4761
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037898.4"
                     /locus_tag="FE326_RS04055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03975"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_172764157.1"
                     /transl_table=11
                     /translation="MIGPKMKARRKTLGLNQRELAEGICAQALISKIERESSSPSSKIL
                     QKLSDRLGVPVTYFLSDSDDGDIEAAKNKENYKRLETLLRAELMKGEYQTISMMMAINA
                     DLIAACRDDYFINLFKWLQGVLYYRIDRNPDKALGLLKEIESQVADEYIELHIDILMSI
                     GMIYDELSDDERASDAYQEATFLLNQSISFKKRVHVLRNHALSLKNMDEMKEALKYVLR
                     AIEVLIKHKSLHMMGDLYLLKGNLFERLGDTEEATQAYEDAIWVFKTLNIKESLTVATV
                     ALHNILPNNKLNN"
     gene            5001..5297
                     /locus_tag="FE326_RS04060"
                     /old_locus_tag="FE326_03980"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850392.1"
                     /locus_tag="FE326_RS04060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03980"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_111974587.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3e-18, bitscore:
                     63.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTTNKLSKTLLAVAAIAALAAPVSAASRFVSGGVWTYGAGGGGA
                     FSNYYHGQRRHSSTVVSLSNSKSKKAWAKKERTSKAFIPTKFGEKAAFYYGVD"
     gene            5389..7413
                     /locus_tag="FE326_RS04065"
                     /old_locus_tag="FE326_03985"
     CDS             5389..7413
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="FE326_RS04065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03985"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_004636433.1"
                     /transl_table=11
                     /translation="MIKKIFVVVSVFLTLVLLLTGINSHKHQLDFSQYKAVNIFGIQED
                     VHVADRETVTERLEQFAREHDIVIARRIVEPNETGEVFFTYDVYGQGDLPDELEVASEE
                     SAGKSDLANSYLIVSGEVDTELLRSEFDSMGFLAITSGEASPINLIMPLIAEMSTLISF
                     VIFILTFAALTIIYRVKDLRLAGIRLLAGESSWQVTLRPVKEDLILIVSSYVINLMIGL
                     VSLFALDLATMLNISLIAIGLLVLPLMYSGISVGLSIIYLMSLRRHMLVDLLKGKLPLY
                     RLISLMLVGQMIAVVVVGWSINETIVATSKYQELLSGQQIWQSNPDLVTLSAGMSADGG
                     NASESSRRDKIWYNFAHDAVENYNAMLVRNNLEQYVLNDVSDGIHKDDYVPIGNTIYVT
                     PNYLDREGIDVSPTIREKLHHLEQGEFGLLLPEKLKSESEKYIEIFTDDMNSYGKAELD
                     ADSEQLYTFQAVVDYVPNNQKRFLYNDAEIVRTQFLDDPIIVVITPKSMGETLDTFLTW
                     GADVASMLYSDYEGTIDLLKQHGVYEWVSYVEGGQLKYLQNMASMKTNLIILIAGAVLS
                     LVTSILLFVSMNILYFEQFRRELFIRRIAGMSPWENHQYYLMAQLGVITVSSGISWYLT
                     GSIGVASGTFGLFTVSALGALFIQARREHHTAMTVLKGK"
     gene            7416..8048
                     /locus_tag="FE326_RS04070"
                     /old_locus_tag="FE326_03990"
     CDS             7416..8048
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="FE326_RS04070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03990"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_172764158.1"
                     /transl_table=11
                     /translation="MLEVQNLVKAFDGRRIFDRVQVRFEPGKSYALVGASGSGKTTLLN
                     ILAYLEPFESGQVLYDGKSLTEVKSQHYFRDHMGYVFQNFGLIESQSVKDNLDLGFVGK
                     KLSKSEKISRQKRALEQVNLGYLDLKQKVFTLSGGEAQRVAIAKLILKNPPLILADEPT
                     AALDETNGKEVIQMILSLLSEERTIIIATHDPLVWEQVDEVIDVTGL"
     gene            8236..8571
                     /locus_tag="FE326_RS04075"
                     /old_locus_tag="FE326_03995"
     CDS             8236..8571
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="FE326_RS04075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_03995"
                     /product="response regulator transcription factor"
                     /protein_id="WP_244325296.1"
                     /transl_table=11
                     /translation="MPVYEYQAHKLELSGFVNKNIDTEDLLSLLKHVKDGGRYFTTENW
                     FIDELTPRERELLEAIATGRKRKIIAEEMYISERTLTNHMQSIMDKLEVNSTIEALQKA
                     RELGYLK"
ORIGIN
        1 tactaaaaat aaacacacag gttattgggt actcgtcgcg gcgttttcgt tgcaattaat
       61 tccactactc ggactagtta tattggcggt gttgtctagt cagttattta ctcaggtatg
      121 gttatttttt atggctgttg cttcaacact tcttatgcta attgctggct ttaatcataa
      181 agctgttgta ggtcaggcat cgaaatggtt gagcttaata ttctgtactt tactctatat
      241 ttatattttt gtgcttattt tagggttaaa tctaagttta gtgttattta tattatatat
      301 tatcgtaagt attatgtata tattgacatt aactgacata tttggcaatc atcaagtgaa
      361 tgctgtcagg ttaattaaac gggaagaggc tctaaaacaa gatttctcca attatttaca
      421 cgatgatgtt ttgcaagata ttaatgtatt gatcaagatg acaacgttgg agccgtctga
      481 gaagacacaa gcgttcttgc gggagcaact gtcagcttta aatgatcaat tgcgccagca
      541 gatgaatcag tatagtccac aattatccaa gcacttaact ttgcaagaga actatcgtct
      601 gcttgtgcgg tcactagagc agacatatcc gaatcaagct gtgtcgacat ctttttcgat
      661 gaatcggaat ttgactttac cagagccgta tgatgtgcta gtttatcggt ggctcaggga
      721 gttgattcat aatgtgtaca agcatgcagg ggcgaatcag ttagatattc atgtggcagc
      781 gtacgatgag ttgataattg tgttggtgga agatgatggg tgctttaaga agggatcaca
      841 tcttaagatt ggtcatggat tgtacgtgat ccaagagcaa gtggaagcaa ttggtggcca
      901 actgtatata gaggcgaatc gtccacgggg gttgcggatg cgggttgagt tcagtgtgat
      961 gggaggcgag ataattgaag attttgttaa ttgatgatca taagttattt tcgcaaagtt
     1021 tggcattagt tctagatcag acaacaagtg atgtgcaagt ggatatgatt aatagtgaag
     1081 gggagttgcc tgatgatcta agcgagttga cagtatatga tgtgttagtg ttggatatta
     1141 atttagataa gggattcagt gaagatgggt tcgagttggc agaacgggtg cgtgctgtag
     1201 cggtagattt gccaattttg atgttgactg gttttgattt gccggtctat gaatatcagg
     1261 cgcataaatt ggaattaagt ggatttgtca ataaaaatat tggtaccgaa gatttattga
     1321 gcctactcaa gcatgtgaaa gatggtgggc gtcattttac aacggagaat tggtttattg
     1381 atgagttgac accacgcgag cgagagttgt taggtgctat tgcgacaggc aagaagcgga
     1441 agatgatcgc tgaagagctt tatatttctg agcgcacgtt gaccaatcat atgcagagta
     1501 ttatggacaa gctagaagtg aactccacga ttgaagcgat ccagaaagcc cgggagttag
     1561 gttatttgaa gtgatcggtg gcgggagaaa gatgactttt ggggtatacg gatcttgcgc
     1621 tttgaatgat tgcttgattt actcatgcat tgcgctgaat tggggagtaa actgggggta
     1681 gattaggtaa tggaggagat tatgatgaaa tatgcaccgt atagtctgct agccttagca
     1741 ggtatattag tttatctgac agtgaatcgc tatattacaa tgcacagcat taatgtcgtt
     1801 cccccgattg gcgcaatctt gtgtgttatt ttctttttcg tctggcgcaa tcacttgaag
     1861 cagcaagggt attatgatga ttaaatagat gaagtctcag tgaaggtgct gaggcttttt
     1921 tattggagta gacttaagat aatatcaagc agctgctggg tgacttcttc gagtgtgtct
     1981 aggggaatgg tggatagctc taatagcaat gggaagtgat cactgtcaag tgagttgggc
     2041 aggtggtgtc gttggcaggt tttgattaag tcacgcaggt tgatgtgatt agggagcggc
     2101 agatgtgtct tgagagcgcg cggcggtagg tagggtaagt gctggttaat tgggtgattg
     2161 gcaacgatag agtggaacag ctctaatttc gcactgtaat gttggtggag ttgctttaag
     2221 tggctatcca gcatgcggtt gtcgatgaga tagagttggg acgatttgtt ggccggcagt
     2281 gagttatcca aaggtagata attagtttgt gtggtattga gatatttgat tgaagtataa
     2341 aaaaactatg accaaagtgt tgtaaccgga ttctttaagt ggtaaactgt tgttggaaac
     2401 aaattaatga aagtagaatg taccgctgtc gaacgattaa ttcaaagggg tgttaatatc
     2461 aaacataaac agttacctaa gatatgtttt tggttaggag ttgttgtgtt tactgtagcc
     2521 gtgctgcttc caactgattt attccaaagc atcacctgga ttgagaaaat acttggtgaa
     2581 ttaaaaccag tcggattagc gaccatattt ttgttgccaa ttatcgggag tgtaggagtg
     2641 gttgggtcca ttatgaacaa atccgtcttg tacggcagtt tgaatgttgc attgattctg
     2701 tcattcccgc tgatgatatt ggtttctaat attgtgcagg cattgtttta aatgatcaaa
     2761 aataagttat ccattgtatc agtaagattg aacaaagaag aagagaaagc gataaatagc
     2821 tatgccacat taacgggaca gccagtatca caactgctta aacaagcact tcttagagag
     2881 attgaagaca aggtagatta tacaattgga gtagaagcgc tagaagaaca cgagaaagat
     2941 ctacagacat ttacgattga tgaagtagct ggggaactag gaattgagtt gtgagttgat
     3001 cgtattagct ctagcagttg ggcacggtaa agttatttac aagtagtacc agttacaata
     3061 aaaagacaag ctatcagaga gatgtctcag tggcttgttt ttttaatatg tcaagttttg
     3121 tcagtgagca actgactcat taatagctga acttcttggg cgccaatagt atcgccacct
     3181 tgttcaaata attgaatcgc tcgcttgaaa tatttccttg caagagacgg cttgtgttgg
     3241 gcgacgcata tttcacccat accacgctga gcgcatgctt gagcaattaa atcatctgtt
     3301 tgttctgctt gtgttaaact ttgagccata aaagagactg cttcgtttag ttgttgcaat
     3361 ttcattctta agtagccttg ttcgtagtta ttgacggact gtttaagtaa gttattagga
     3421 aagtgctgtt caataatacg tgcttcttgt tgaatcaatt ccaatgcttg tccgtaatct
     3481 tcacgcatgc gatagaccat cgccagttga tgtaatgcta tatgttcttg ttctatatca
     3541 tgatttttac ggcttaattg tagccactgt tgataagtag attcagcttt atctatttta
     3601 ccgtcttcta gatagatata gccgagtaag ttcaatgcat tgtaatcagt cgttgcttgt
     3661 tcatttatat aatcatcaac gagttgtttg gcttttggga tatttccgcg cgaaaattgt
     3721 ccccatgctt gatttatcat ttatttcacc ccatttgtct attgatttat tatactggat
     3781 tcagaataat atttcgagta tttattaaaa ataattattt taatcataac tatattattt
     3841 tataataaaa ttaataaaaa tattggagga attatgatga ttggaccaaa aatgaaagca
     3901 cgtcggaaaa cattgggatt gaatcaacgt gagttagcag aagggatatg tgcacaggcg
     3961 ttaattagta agattgagcg agaatcgtcg tcaccaagta gtaaaatact acaaaaactt
     4021 agtgatcgat taggcgtgcc ggtgacgtat ttcttaagtg attcagatga tggtgatatt
     4081 gaagcggcta aaaataaaga aaattataag cgactagaaa ctttattacg cgctgagtta
     4141 atgaaaggtg agtatcagac aatctcaatg atgatggcga ttaatgcaga tttaattgct
     4201 gcgtgtcgtg atgactactt tattaatcta ttcaagtggc ttcaaggtgt attatattac
     4261 cgaattgacc gtaacccgga taaagctctt gggttactca aagaaatcga aagtcaagtc
     4321 gctgatgaat atattgagtt acacattgat attttgatga gtattgggat gatttatgat
     4381 gaattaagcg atgatgaacg ggcatcagat gcctatcagg aagcaacgtt cttactgaat
     4441 caatctattt catttaaaaa acgggttcat gtcctgcgta atcatgcact aagtttgaaa
     4501 aatatggatg aaatgaaaga agcgttgaag tatgtattga gagccatcga agtgcttatt
     4561 aaacacaaat cactgcatat gatgggagat ttatatcttt taaaaggtaa cttattcgag
     4621 cgattaggcg atactgaaga agccacccaa gcttatgaag atgcaatttg ggtgtttaag
     4681 acactgaata tcaaggaatc attgacagta gcaactgttg cattgcataa tattttacct
     4741 aataataaat taaacaatta aattattaat ataaaaaaca accaaaaaac atttttgaaa
     4801 ttttgataaa aaaagtgttg atattgtatt taggtgggta tatacttatc tcatacctaa
     4861 gatatccagt tatcttaaga cttgtcaatg agaagcgata aatttatgaa aggttgcaaa
     4921 aagagtggtc gcattatcta ttaggagcca ttaagctaac caatattcat aggtaaactc
     4981 atataaagga ggttttcaca atgaaaacca caaacaaact atcaaaaaca ttattagcag
     5041 ttgcagcgat tgcggcattg gcagctccag tatcagcagc ttctcgattt gtttcaggag
     5101 gagtatggac gtatggagca ggaggtggtg gagcattctc gaattattat catggacaac
     5161 gacgacactc ttcaaccgtt gtgagcttat caaattctaa aagcaaaaaa gcatgggcta
     5221 aaaaggaacg aacatcaaag gcatttattc ctacaaaatt tggtgaaaaa gcagcctttt
     5281 actacggtgt tgactagata gaatcaatat ttttttacaa tctgattact tttaaaataa
     5341 caaagtaatc agattgtatt tatagataga tggaaagaag aatgagcaat gataaagaaa
     5401 atatttgtcg tagtatctgt ttttttgact ttagtattgt tattaacagg aataaattca
     5461 cataagcatc aactggattt ctcacaatat aaggcagtta atatatttgg tattcaagaa
     5521 gatgtacatg tagctgatcg tgaaactgta actgaacgat tagaacaatt tgctagggag
     5581 catgatattg tgattgctag acgtattgtt gaaccgaatg aaactggtga agtatttttt
     5641 acgtatgatg tgtatggtca aggtgacctc cccgatgaat tagaagtagc ttcagaagaa
     5701 agtgcaggaa aaagtgacct ggccaatagt tatttaattg tctcaggtga agttgatacg
     5761 gagctgttga gaagtgaatt tgatagtatg ggattcctag cgattactag tggagaagct
     5821 tcgcctataa atcttattat gcctttaata gcagaaatgt caacgttaat tagttttgtt
     5881 atatttatat taacctttgc cgctttaacc atcatctatc gagtaaaaga tttaaggtta
     5941 gcaggcattc gattgttagc aggagaatct tcatggcaag taacgctacg accagtgaaa
     6001 gaagatttga tcctaattgt atcatcatat gtgattaatt tgatgattgg attagtttct
     6061 ttattcgctc ttgatcttgc aaccatgctt aatatcagtc ttatagcaat aggtctgctg
     6121 gttcttcccc taatgtattc agggatatca gtgggcttga gtataattta tttaatgagt
     6181 ttgagacgac atatgttagt tgatctactt aaagggaaac ttccattgta ccgtttaatt
     6241 agcttaatgt tagttggtca gatgatagca gtcgttgtcg ttggatggag tattaatgaa
     6301 acgattgttg ctactagcaa gtatcaagaa ttattatctg gtcaacagat ttggcaaagc
     6361 aatcctgatt tagtgacttt atcagctgga atgtcagcag atggggggaa tgcctccgaa
     6421 agtagccgac gtgataaaat ttggtataat tttgctcatg atgcagtaga aaattataat
     6481 gctatgctag ttagaaataa cttagagcaa tatgtattga atgatgtgtc agatggtatt
     6541 cataaagatg actatgttcc tataggtaat acaatttatg taacacctaa ttacttagat
     6601 agagaaggaa ttgatgtatc tcctactatt cgtgaaaaat tacatcattt agaacagggg
     6661 gaatttggct tgctccttcc tgaaaaatta aaatcagaaa gtgaaaaata cattgagata
     6721 tttacagatg atatgaatag ctatggtaaa gcagaattag atgcagatag cgaacaactt
     6781 tatacttttc aagctgttgt cgattatgta cctaacaatc agaaacgttt cttatataat
     6841 gatgcggaga tagttagaac acaattttta gatgacccga ttattgtcgt tataactccc
     6901 aaatcaatgg gagaaactct agatacattc ttgacttggg gggcagatgt ggccagtatg
     6961 ctttactctg attacgaggg aacaattgat ttacttaaac aacacggtgt ttatgagtgg
     7021 gtttcgtatg tagaaggcgg acagttgaag taccttcaga atatggcgag tatgaagact
     7081 aacttaatca ttctcattgc cggcgctgtc ttaagtttag tgacatcaat tctactgttt
     7141 gtgtcaatga acatattgta tttcgagcag ttccgtcgtg agttattcat tagacgcatt
     7201 gctggcatga gcccatggga aaatcatcaa tactacctga tggctcaact cggagtcatt
     7261 acggtttcat caggcatctc ttggtattta accggcagta tcggggtggc ttcaggtaca
     7321 ttcggcttat tcactgtcag tgcattgggt gccttattca tccaagcacg gcgggaacac
     7381 cacacggcaa tgaccgtact gaaaggaaaa taatgatgtt agaagttcag aatttagtaa
     7441 aagcctttga tgggcgccgg atttttgacc gtgttcaggt gagatttgag ccggggaaat
     7501 cgtatgcctt agttggggcg agtggcagcg ggaagacgac tttgttaaat atcttagcgt
     7561 atttagagcc gttcgagagt gggcaggtgc tgtatgatgg caagtctctg acggaggtga
     7621 agtcgcagca ttatttccgc gatcatatgg gatatgtatt tcagaatttt gggttaattg
     7681 agagtcagtc ggtgaaggat aatttggact taggatttgt cgggaagaaa ttatcgaaat
     7741 cagagaaaat atcaaggcaa aaacgagcgc tagaacaagt taatttgggc tatttggatc
     7801 tgaagcaaaa agtgttcaca ttatctggcg gggaagcgca acgggtggcg attgcgaaat
     7861 taatcttgaa gaatccgcca ttgatacttg ctgatgagcc cactgccgca cttgatgaga
     7921 caaatggcaa agaagtgatt cagatgattt tatcgctgct gagcgaagag cgaactatta
     7981 ttatcgccac acacgatcca cttgtctggg agcaagtcga tgaagtcatt gatgtgacgg
     8041 ggttataaga ctgtttaaga gtcaagttta cggcgggcag accgtgaaac ttggcttttt
     8101 tcagggtgaa tattattgtc ttatacataa ttcaggggag gaaatttcga atcgctaacc
     8161 ccttgatatg ataggggttt caccgatcca aaaataaaca tcgacagacc aaaccctttc
     8221 ataataggat ttgatttgcc ggtctatgaa tatcaggcgc ataagttgga gttaagtgga
     8281 tttgtcaata aaaatattga taccgaagat ttattgagcc tactcaagca tgtgaaagat
     8341 ggtgggcggt attttacaac ggagaattgg tttattgatg agttgacacc acgcgagcga
     8401 gagttgttag aagctatcgc gactgggagg aagaggaaaa taattgctga agaaatgtat
     8461 atttctgagc gcacgctgac caatcatatg cagagcatta tggacaagct agaagtgaac
     8521 tccacgattg aagcgcttca gaaagccaga gaattaggtt atttgaagtg ataaaagata
     8581 ttatgaataa atagataaag tctcagtaat tatgtactga ccccaagagt tagagtgaat
     8641 aattctaatt ttttggggtc acatcatttt aagggattgt tttagtcata ttgtggtgcg
     8701 gtcatacact agatagggct acaagctcat ccctcgaatg tattcaattt cttcttaagt
     8761 tgtcttatct tttgaaaaga ttatagacca ataaaaagtc aagttctacg gcgggcagac
     8821 ggtggaattt gactttttaa atgagctaat aaaaatgagt caatcactag ggtttatacg
     8881 agcgtgttct tttggtaaaa taggaattga cgatagggaa gatgagatca tataggggag
     8941 ttaatcgatg tcaaagaaga aattgttatt aattgatgga tatagtattg cgttccgggc
     9001 attctatgcg atgcatagtc agttgcaccg gatgaagaat aagaatggac tacatacgaa
     9061 tgcgctctat gggttccata atatgttaga agcggtgatg gaaaaagaac aaccgacgca
     9121 tgcattagtg gccttcgatg cggggaagac tacttttcgg catgagttgt ttgatgatta
     9181 taagggcggt cgtgagagta tgccggctga gttatccgaa cagatacctt atttgaagga
     9241 attaattgca gcgtatggtc tacagacgta tcaattgccg gattatgaag cggatgatat
     9301 tatcggaacc ttaagtactc aagcatcaga tgaattcgaa gtcgtcatta ttacggggga
     9361 caaggatttg acgcaactgg ccagtgatca gacccgggtt gatattacga agaaggggat
     9421 tacgcagttg aagtcgtata cgccggaatc aatgatggaa gagatgggga ttgccccaga
     9481 acaaattacc gatctgaagg gattatcggg ggattcttcg gataatatcc caggtgtgac
     9541 acagattgga gataagacgg cgcttaagtt gttgaagaag ttccatacag tagaagaatt
     9601 gtatgaacgt attgatgaga tgaatgcctc taagcgcaag gaatatttaa tcgctgagaa
     9661 agaaacagcg ctacttagca agcaattggc cactattatt actgatgcac ctattgagat
     9721 ttcaccggag tcattagctt attcaggaaa aaatatggag gcgttgattg gcttctataa
     9781 ggaaatggac ttcaatagtc atttagaaaa gttagatacg gatgtgtact tcgaagaatt
     9841 acaagatgaa gcagaagcgg ttgaatatga atgggtcgaa gaagtaacgg aagcaatgtt
     9901 cggacagagc gggacgctgt atttcgaaat gttcgaagag aattaccata cggcacctta
     9961 tattattgcg agctggacta atgagaagaa agtctatgtg gctgagccag agacattgct
    10021 agcttctaag gcttttcaag agtggctagt ggatgagtca gctcggaaat atgcgtatga
    10081 tgcgaaagca ttaatggtgt ccttgaagca acgtgaattg gagattacca atattacttt
    10141 tgatttgtcg ctggcatcgt atgtattgac cgctgagaat tctagtggag gcgatgtggc
    10201 ggctattgcc aacaagcatg gatatgtgga tgtcttagaa gatgaagcgg tctacggtaa
    10261 agggaaaaaa gtaagtattc ctaatgataa aacaatc
//
