LOCUS       NZ_CP040419            22697 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3069 chromosome, complete genome.
ACCESSION   NZ_CP040419
VERSION     NZ_CP040419.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 372x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655825.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:32:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,913
            CDSs (total)                      :: 1,844
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 6 of 44
            Pseudo Genes (multiple problems)  :: 5 of 44
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:54:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 561939
            Orig. end                         :: 584636
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040419.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22697
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="2"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..22697
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22697
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12697](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12697
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            1483..1803
                     /locus_tag="FE326_RS02860"
                     /old_locus_tag="FE326_02785"
     CDS             1483..1803
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /locus_tag="FE326_RS02860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02785"
                     /product="transposase"
                     /protein_id="WP_208954528.1"
                     /transl_table=11
                     /translation="MYVKTRNYRTHLKPLLDKFTEWLDHHQYLTKAKLGKAIIYARKML
                     PSVYLLLDHAGLSISNNSAEQAMRIPALGRKNWLFSQSIKGAIANGYFLTVIQTAIANG
                     LD"
     gene            complement(2001..2738)
                     /locus_tag="FE326_RS02870"
                     /old_locus_tag="FE326_02795"
     CDS             complement(2001..2738)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /locus_tag="FE326_RS02870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02795"
                     /product="IS3 family transposase"
                     /protein_id="WP_208954740.1"
                     /transl_table=11
                     /translation="MERPSRRQQSRIAFELTEDHFRLKYVLMVIDLLASTYHYYMAQKA
                     NDPKDHPLEQLIQKIFNDSNETYGYRRITLTLRNMGYCVNHKRVQRLMQKLGIKCMKFF
                     HKSRRYNSYKGKVGKVVSNRVNRRFMTSVPLQKLVTDVTEFKCLKDEKLYLSPIMDLYN
                     NEIIAFKIAKRPTLDIALEPLTEAISRIERHAPYRTTIHSDQGWHYQHRAWISYLKEHG
                     IYQSLSRKGNCLIIHRWRISLGS"
     gene            complement(2702..3250)
                     /locus_tag="FE326_RS02875"
                     /old_locus_tag="FE326_02800"
     CDS             complement(2702..3250)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /locus_tag="FE326_RS02875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02800"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208954530.1"
                     /transl_table=11
                     /translation="MSKTYSYPFKLNVVQAYLDGLLGYHLLAKKYDVSSEQLIQNWVNQ
                     YQLYGEARLKRKKTKKKYRLRFKLNVLKFKLETGASYKEMANAFGIGEPSIIANWKRLF
                     MEGGVQSLNKPIGGPPKMPKSSRSSTQKTKQSKQKTDQVARENAQLREENNLLKIELAY
                     LKKLRERGLSDPREDNNQG"
     gene            complement(3327..3596)
                     /locus_tag="FE326_RS02880"
                     /old_locus_tag="FE326_02805"
     CDS             complement(3327..3596)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /locus_tag="FE326_RS02880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02805"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_208954531.1"
                     /transl_table=11
                     /translation="MIGQKIKDRREYLNLSQRELAEGLCTQALISKIEQGKIKPSSKII
                     QQLSNRLQVPTSYFFRENSNHKSSDVQNFEENQNLLNYKNSYEI"
     gene            complement(3669..4262)
                     /locus_tag="FE326_RS02885"
                     /old_locus_tag="FE326_02810"
     CDS             complement(3669..4262)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="FE326_RS02885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02810"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_208954532.1"
                     /transl_table=11
                     /translation="MKVLIIDQHCFSGKCIFTALTQANTIDTFYFHEPSHDLINKIILL
                     DIDIILLVIHLETLNEFKLAKKIIDETRSKLCFMSALDLLEFKQIAYDIGAFAFFNETI
                     DIDTLITQLKKIHNQSSPSYIINDCRHVLTSQEKLILEKLANGETQKSIAFDLGITTRT
                     IRNHLYSINKKLDTQSTIQAIVKAHSLGIIYFNL"
     gene            4419..4616
                     /locus_tag="FE326_RS02890"
                     /old_locus_tag="FE326_02815"
     CDS             4419..4616
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS02890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_02815"
                     /product="hypothetical protein"
                     /protein_id="WP_208954533.1"
                     /transl_table=11
                     /translation="MKQSDKDTVQGNKEHLVEKNFKIYDEVTDEDLQENGGVAWTSLVP
                     ISLAICPSTKCASITRPCNK"
     CDS_motif       4419..4529
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKQSDKDTVQGNKEHLVEKNFKIYDEVTDEDLQENGG"
                     /locus_tag="FE326_RS02890_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       4530..4616
                     /prepeptide="core"
                     /locus_tag="FE326_RS02890_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-0.70"
                     /molecular_weight="2834.4"
                     /monoisotopic_mass="2832.5"
                     /core_sequence="VAWTSLVPISLAICPSTKCASITRPCNK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKQSDKDTVQGNKEHLVEKNFKIYDEVTDEDLQENGG"
                     /leader_location="[4418:4529](+)"
                     /alternative_weights="2852.4"
                     /alternative_weights="2870.4"
                     /alternative_weights="2888.5"
                     /alternative_weights="2906.5"
     gene            4628..4825
                     /locus_tag="FE326_RS02895"
                     /old_locus_tag="FE326_02820"
     CDS             4628..4825
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386877.1"
                     /locus_tag="FE326_RS02895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02820"
                     /product="plantaricin C family lantibiotic"
                     /protein_id="WP_208954534.1"
                     /transl_table=11
                     /translation="MNKDFDKELLNISGNLKEELSEADLEQISGAGVGKWIRKLSKGNT
                     GKVCTLTWECGICPSHTCFL"
     CDS_motif       4628..4720
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MNKDFDKELLNISGNLKEELSEADLEQISGA"
                     /locus_tag="FE326_RS02895_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       4721..4825
                     /prepeptide="core"
                     /locus_tag="FE326_RS02895_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-0.80"
                     /molecular_weight="3616.3"
                     /monoisotopic_mass="3613.9"
                     /core_sequence="GVGKWIRKLSKGNTGKVCTLTWECGICPSHTCFL"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNKDFDKELLNISGNLKEELSEADLEQISGA"
                     /leader_location="[4627:4720](+)"
                     /alternative_weights="3634.3"
                     /alternative_weights="3652.3"
     misc_feature    5250..5263
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.91"
                     /tool="antismash"
     gene            5449..7907
                     /gene="lanM"
                     /locus_tag="FE326_RS02905"
                     /old_locus_tag="FE326_02830"
                     /pseudo=""
     CDS             5449..7907
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DUF4135"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024964.4"
                     /locus_tag="FE326_RS02905"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02830"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /pseudo=""
                     /sec_met_domain="DUF4135 (E-value: 7e-56, bitscore: 186.8,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYLVYTTITFFKEFIFEIVKHFKNDKSILVQNTLIIEEDKIKCM
                     ILGQGDSHNKGKTVVKIKMSTGKTLYYKPRNLEVDLLFNKVIQLLSEKENIDLCTYGVC
                     SLENHGWVEEVEYKPVKTLDELFMFYKNMGSLLAILYALNAVDIHIDNIIANGEIPMLI
                     DCESLFHSSEYHEEEDNATRYLNNYLSQSVLSIGVLPFIFGTGSTDISALSDQKNKKLQ
                     LKFQQ"
     misc_feature    7940..7953
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.26"
                     /tool="antismash"
     gene            8026..9729
                     /locus_tag="FE326_RS02915"
                     /old_locus_tag="FE326_02840"
     CDS             8026..9729
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="FE326_RS02915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02840"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_208954538.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6.2e-17, bitscore:
                     58.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKDKQEIIGRDGLSLADLKKIAKTNAGNLRVFKVYDITKYLDTY
                     EITQPLLCYINNSHYIVVTCKKKDRIWVNDPQVGRYNISEQEFSNLFSGYIAILEKNKK
                     NKDIFEFSSKKNARLFSYITTHSSVVIQLIFWTLVFQVLNILFPAMIQKIIDQEINITI
                     FSLFMILGLSIVYYLISKYQLKLATKLQIIINKLLTIDFVKKVFSIKSSFIEINSIGYF
                     TNSLSNINTIKEILVSLSTLIVINVTLMLIYTIALIYFSPILTLIVYGFVIMQGIVLWK
                     AAPKIRDFIGGEIKNNIDFQNYYIESLKNYNNIKSMGDPKYVVNFILDKFTGQLHSFEK
                     RMDIQIKLTSFLGTVQWLLPFIVLIAGYLLMDSINLTMGSLVAISAIALRIVTPLASAS
                     EVYQSILMMKEIFENIDNVLTQPEENADKGNSIDTMPFPIKFNDIGFSYGNTEIFKNIT
                     LNIERNDKIIIKGKSGEGKTTLLKLLTTLYAPTVGKIYFGGKELKNYKLNHIRSKIGYM
                     SQDYSLISGTIMDNITMFDDRISLKDVEKAAQLVCIDEFINQMPMNYYTF"
     gene            10001..12697
                     /gene="lanM"
                     /locus_tag="FE326_RS02925"
                     /old_locus_tag="FE326_02850"
     CDS             10001..12697
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="FE326_RS02925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02850"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_208954540.1"
                     /sec_met_domain="DUF4135 (E-value: 2e-61, bitscore: 205.0,
                     seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 3.1e-29, bitscore:
                     98.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTTNKLTSLFIKENYLNSIDDNIQVIIEGEQTFLNKDKISKTLY
                     EYFYNTIYEVSIKQLLKWFHSSFDYIDETDKQYNINKFISFKPFYSTREFLILDKLIVK
                     IIENKTNFAKEVISNFQKSYENIKSKYRLGESINLCIEDLIFEVGDLHNSNKSSCVIIT
                     NQKLKLVYKPRTAANDKFFYEIVNFLENHLNLVQTEYPSLIISEATFSIFPFIDKQELT
                     DNAEAEEYYYRLGISTMVLYVVGATDIHFENIMSNKQFPIFIDLETLFDVTKIKIQEES
                     NEFNSELLQSVLTSHIFDYSLNPNQTDIVGIGGYTTSVNLESEEKLINKDTDEMHLSFI
                     QTKKDIFNVPRLANCFLEITEKKTQFIKGFYDAYNIILRHKNYLKKIIKDYSSSIILRT
                     LIRPTYIYHNYRRKILEDLTIIHKNERLWKKLEQNSMVNKEIINLEKRALTRYDIPFFY
                     CIMNGCSLCSEGARVEGFLPITPLEYYLNKLEKITTEDCLVQLWYLNQSIEIYNNYLFK
                     KINTENKNDIKMYLERRIGAHLAEDVSFCSIKMCYNSDIVLSPMNYDLYDGLYGLKFMD
                     KIFSERYNVHLIRDRNKIQNIIDKLSKINLANFSAYSGQFSQLKYNLLKFEISNSNIDF
                     KNDWITYSQLVEKINKLSTPNIDFLNGISGIISLMIELYHKTKDERIIIDIERTSNFLW
                     EEYQESPIQNSIISFAHGAIGIEYVFSKVVPLLSDDSKIKQKLIRHLDEKLTEIRNYIQ
                     HVIINETEINYTWCNGLTGFLVAWLAINKHLNCQFSELEKKLINKYIDNYTFKMLDDSI
                     CHGHAGNLLSLNYILDRNLNKGYRRGDLIDLKIQIERCLQNKIMYNKICSGYEYKDIKS
                     FSLFLGDLGVYASYILSTEGISSSILY"
     gene            12716..13576
                     /locus_tag="FE326_RS02930"
                     /old_locus_tag="FE326_02855"
     CDS             12716..13576
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="FE326_RS02930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02855"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_208954541.1"
                     /transl_table=11
                     /translation="MLSMQSISKKYGELYALKDAELEIKKGEMVALIGNNGSGKTTLMK
                     IISGITKASTGTFEWETNRQTNLIETPEFFDYLSGYKNLEYFRIQRGIQDQHIIEDILE
                     RVGLPNDRKKFKQYSLGMKQRLAIGLCLLGNPEFMVLDEPTNGVDAQGIIDIRNLLLTL
                     NKEEQLTMLISSHNINELYNIATRFVFIKSGEIKKDISKSELNENIGTFYQVNIGRIQD
                     IEALINDTVLQSNYYTIDDKNTLKLVKNFETSQFIEQLSQKELIDDISEVQVTLEDYYL
                     EIMKG"
     gene            13584..14339
                     /locus_tag="FE326_RS02935"
                     /old_locus_tag="FE326_02860"
     CDS             13584..14339
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS02935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_02860"
                     /product="hypothetical protein"
                     /protein_id="WP_208954542.1"
                     /transl_table=11
                     /translation="MVKKYIYSELYRIKHIRMYIALFFITILFPIATTAFLSIISLQQT
                     EPTTKEFGLYSLYMLIFIGLYLAYPIVICANTVKPKYVERQILSSGITKNILYFSDLFY
                     INIIQLIMLVLYSVSSIISTTLLLESAPGAEDSLDLVTVKEFIIIMCCIYITMILSSIV
                     LYSLQLVLSNKIFATGLFMFLVYVIPLVIAQIRYFSDIADSVAQIIPFLQVAFFYKGDS
                     PLSMLLLIHGIWIAVTIMICNTIYNKIEA"
     gene            14366..14548
                     /locus_tag="FE326_RS02940"
                     /old_locus_tag="FE326_02865"
     CDS             14366..14548
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS02940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_02865"
                     /product="hypothetical protein"
                     /protein_id="WP_004636946.1"
                     /transl_table=11
                     /translation="MKESLFFVSLLFLAVFLILGVYWLTQRWVYDVDVRGLRKWILTSF
                     VLYAITYGISLYLIN"
     misc_feature    14764..14784
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.95"
                     /tool="antismash"
     misc_feature    complement(14765..14785)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.44"
                     /tool="antismash"
     misc_feature    complement(14896..14910)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.87"
                     /tool="antismash"
     gene            <15051..15542
                     /locus_tag="FE326_RS02945"
                     /old_locus_tag="FE326_02870"
                     /pseudo=""
     CDS             <15051..15542
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /locus_tag="FE326_RS02945"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02870"
                     /product="DNA topoisomerase"
                     /pseudo=""
                     /transl_table=11
                     /translation="HKEDRIGVAKLVGDKGLMKKLGLGTEATRSGIIETIKYRKYIEND
                     GKTKLKPTKKGLLLYKITKYVLVSSPEMTTKWEAYLEQIGQGNKSPDPFIKRVKESIKP
                     TFAELNDNEAGTESVQSVKTQNLEEIGNYQVRTKNKLFEVTPEGASEHFPLWRNIAGHI
                     "
     gene            complement(15980..16741)
                     /locus_tag="FE326_RS02950"
                     /old_locus_tag="FE326_02875"
     CDS             complement(15980..16741)
                     /NRPS_PKS="Domain: PKS_KR (6-163). E-value: 8.1e-10. Score:
                     30.8. Matches aSDomain:
                     nrpspksdomains_FE326_RS02950_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907568.1"
                     /locus_tag="FE326_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02875"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_112787852.1"
                     /transl_table=11
                     /translation="MTKTYSLITGASSGIGRDLAYKLASEGHNVILVARREQRLKEIAV
                     DIEENYHVSAEYLVQDIGDVARLDDFYEKTKAFNIDLWVNNAGISVSGDFLEMDPNDIL
                     KMLKLNTGALAKLSILYLQDYADEPVQLLNVSSVAGYALMEGAPLYSVSKHFVSTLTEN
                     LAHELANKNKPARAKVLAPAATETEFVGVVRGDAEADVDYSDVFERYHTSEQMAEFAWE
                     LLESDKVVGIVDLDDFEFKLTDPQFKYAGGN"
     aSDomain        complement(16253..16723)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FE326_RS02950_PKS_KR.1"
                     /evalue="8.10E-10"
                     /label="FE326_RS02950_PKS_KR.1"
                     /locus_tag="FE326_RS02950"
                     /protein_end="163"
                     /protein_start="6"
                     /score="30.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="LITGASSGIGRDLAYKLASEGHNVILVARREQRLKEIAVDIEENY
                     HVSAEYLVQDIGDVARLDDFYEKTKAFNIDLWVNNAGISVSGDFLEMDPNDILKMLKLN
                     TGALAKLSILYLQDYADEPVQLLNVSSVAGYALMEGAPLYSVSKHFVSTLTEN"
     CDS_motif       complement(16634..16723)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FE326_RS02950_0001"
                     /evalue="1.80E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FE326_RS02950"
                     /protein_end="36"
                     /protein_start="6"
                     /score="14.2"
                     /tool="antismash"
                     /translation="LITGASSGIGRDLAYKLASEGHNVILVARR"
     gene            complement(16809..18707)
                     /locus_tag="FE326_RS02955"
                     /old_locus_tag="FE326_02880"
     CDS             complement(16809..18707)
                     /GO_function="GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634574.1"
                     /locus_tag="FE326_RS02955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02880"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_208954544.1"
                     /transl_table=11
                     /translation="MLNYLKTNREGQFLVIGILLTVLGFSLSIMDLTSSRWAFYGAIVF
                     LGFYAAKEALVETFKHKAPNDALLMILSALGATLIQYESEGAILLLIFAGAEVLEDYVT
                     DKATDSISELMSSVPNQAHIMRENGKVDRVPTDSLAIGDTVIVQKGAQLPIDGTIDRQA
                     VLNESALTGESIPVEKSSGDMVYAGTLNEGDVFYLTVTQTSNDTIFSNIIHMVEQAQQQ
                     PSKKARWIDTFESYYVTGVLIAVPLFIIGLFFLQELSWQDAFYRGLVLLTVASPCALVA
                     SITPATLSAISNGAKNGILFKGGKVMERFGELKTLYTDKTGTLTQGEFSVTDYELADDY
                     ISALVYMEQQSNHPIAQAIVAHFQSEHIDLPTGSDQIEEIAGAGLRMGNITVGKPDLFK
                     QSTGIETVQAAIDSTDTTVIIAEHDRIVGYVNLADKIRVNSQQAVQQFQAAGVKVVLLT
                     GDQQRVAEAVATDIGIPKTYAELLPEDKMNFIQSSQSSNEVVGMIGDGINDAPALAHAD
                     IGIAMGSGSQAAMESADIVIVKNDLQKLFNSYRLSQRLDKIIKQNLFFAIGVIITLITL
                     NILGWLDLPMGVVFHEVSTILVILNGLRLLRNSSTAAVEINDTAQSTADESETVTQ"
     regulatory      complement(18824..18921)
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    18996..19011
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.19"
                     /tool="antismash"
     gene            19258..20358
                     /gene="lipA"
                     /locus_tag="FE326_RS02960"
                     /old_locus_tag="FE326_02885"
     CDS             19258..20358
                     /EC_number="2.8.1.8"
                     /GO_function="GO:0016992 - lipoate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lipA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634572.1"
                     /locus_tag="FE326_RS02960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02885"
                     /product="lipoyl synthase"
                     /protein_id="WP_111973909.1"
                     /sec_met_domain="PF04055 (E-value: 1.7e-12, bitscore: 44.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIHIKMPKLLPNMEMGKLVAWVVDEGEIFEAGDPLYEVETDKVV
                     NIIEAEMDGLMMKHRYEEGDDVPVGAIVAEVGPRSSKPDWLRVSYNGSAIKNVSNILEE
                     NRLTTVCEEANCPNIGECFGNNTATFMIMGEKCTRGCKFCNIERTARPGQLDPLEPIRV
                     ADAVRKMKLEHAVVTQVARDDLKDGGAAHMAETVRQIREINPETTIEVLISDLRGSYEA
                     LEDVLAADPDVLNHNMEMPKEWYRKVQPQCNYEQSLNVLRNSKKISPNTLTKTGFMVGL
                     GESDEEISNLMDDILATGCDILTINQYLQPSSEHHKLARYVTPEKFNEYEEMAREKGFK
                     YVVAAPLVRSSYKAAEAFEHAKAKQA"
     gene            20362..21363
                     /locus_tag="FE326_RS02965"
                     /old_locus_tag="FE326_02890"
     CDS             20362..21363
                     /EC_number="6.3.1.20"
                     /GO_function="GO:0017118 - lipoyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /locus_tag="FE326_RS02965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02890"
                     /product="lipoate--protein ligase"
                     /protein_id="WP_208954545.1"
                     /transl_table=11
                     /translation="MLFINNDSNDVYFNFALEHYFVTEKQMNEPLLVFWRTEPTLMVGK
                     YQNIYEEVDVNYAKQHDINIVRRPSGGGTIYTDLGSWQFTFIHYQTKREIFFDDFTQPV
                     IEALNRLGVPAEFNGRNDIVIENKKISGNAQYKEKGITVHHGSLLFDTNIEEMVRSTTV
                     DEHKIISKSIKSIRDRVTNIAEYLSNDFTAMDFKQYMVQELMGNEATIYHLTRTDIARI
                     NELADQLFRSLEAKYKRNPTFTRTTTHYLAGGKLSVSLNIKSGRIAELQLTGDFFSGED
                     IEEFCQKLVGTRLDEQAIREKITELTDQSLIYNISNEEIATAILDIPQRNID"
     gene            21379..21723
                     /locus_tag="FE326_RS02970"
                     /old_locus_tag="FE326_02895"
     CDS             21379..21723
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142512.1"
                     /locus_tag="FE326_RS02970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02895"
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="WP_004634568.1"
                     /transl_table=11
                     /translation="MMENLNVELLNEAQTHQEVLHQQLTQVIDPELGIDVLNLGLIYKL
                     DLNKKGDCKVTMTLTSMGCPLADEIMNGVRFALLDCDPVEFVNIDLVFSPAWSTSHMSR
                     YARISLGVRA"
     gene            22102..22476
                     /gene="arsD"
                     /locus_tag="FE326_RS02975"
                     /old_locus_tag="FE326_02900"
     CDS             22102..22476
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /gene="arsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634566.1"
                     /locus_tag="FE326_RS02975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_02900"
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="WP_112789158.1"
                     /transl_table=11
                     /translation="MKKMVIFDPAMCCSTGVCGPSVDENLLRVSTVINKLKKKDVVVER
                     HNLANDPKAFVENELINQIIDSEGTEALPITTVDGEVVKKKEYPTNDEFCSFLDIPKAY
                     LDSSKEQSTKKCCCGKGNCC"
ORIGIN
        1 tctatcaccg cagctcaagg aatacttgaa agtggatggg gacgatcaga tctggcaact
       61 ataggaaata atctctttgg ggtaaaagga tcataccaag gtaatactat aaatatgaat
      121 actagagaag aaactccaac tggtaaagat tattatataa atgctggttt cagattatat
      181 cctagttggg ctgctagtat tgaagatcat ggtaaatttt taaatgaaaa ttcaaggtat
      241 cgtaagttaa taggagaaac tgattataga actgtagctc atctattaca gcgagcagga
      301 tacgctacag cgggaaatta tgcttcttca ctaataggac ttattgaatc taataaatta
      361 tctttatggg atcaggaagc attctctggg agaaaattat ctccaacagt aagttctaca
      421 ttatctggga atagctctaa acaagtatat acagttgttt caggtgattc actttgggga
      481 atagcaaata aaaatggtat tacattagat cagttactac agtggaataa tttgaataga
      541 aattctatta ttcacccggg agataacttg tttgtatcta gtactaaaaa taatgtagtt
      601 tcaagtacca ctagccatac atctacagct tcttcaaata aacaaccaac agctaaaggt
      661 aaaacatata cagttgtttc aggtgattcg ctttggggaa tagcgaacaa aaatggtatt
      721 acattagatc agttactaca gtggaataat ttgaatagaa attctattat tcacccggga
      781 gataacttgt ttgtatctag tactaaaaat aatgtagttt caagtgctac aagtcataca
      841 cctacacctt cttcaaataa acaaccaaca actaaaggta aaacatatat agttgtttca
      901 ggtgattcac tttggggaat agcgaacaaa aatggtatta cattagatca gctactacag
      961 tggaataatt tgaatagaaa ttctattatt cacccgggag acaacttgtt tgtatctagt
     1021 actaaaaata atgtagtttc aagtgctaca agtcatacac ctacaccttc ttcaaataaa
     1081 caaccaacaa ctaaaggtaa aacatataca gttgtttcag gtgattcact ttggggaata
     1141 gcgaacaaaa atggtattac attagatcag ctactacagt ggaataatct aaataaaaac
     1201 tccattattc atccaggaga tacgttatta attaataaat aatatatatt attttaaagt
     1261 ggtaactaag tacgtaattg agtaggcttt ttccggtaat tgaccgatta tagcctactc
     1321 aattttgcta ttaatcaggt cattattgtc gtaattaata gacgttagag cccaagcatt
     1381 taattatttt tttcgtcagc gcccatgatt taggcgacta gttccacctc aaaaaatcct
     1441 ctaaaatagt taatgtaaag taaaaactat tgagaggatt ttttgtatgt caaaaccaga
     1501 aattatcgta cccacttgaa gccattgctt gataaattca ccgaatggtt agaccatcat
     1561 cagtatctaa caaaggctaa actgggcaag gccattatct atgctcgtaa gatgcttccg
     1621 agtgtgtact tattactcga tcatgcgggt ttgagcatta gtaacaattc cgctgagcaa
     1681 gctatgagaa tacccgcttt gggacgtaaa aattggttgt tttctcagag catcaaagga
     1741 gcgatagcaa atgggtattt cttaacggtc atccaaacag ctatagcaaa tggtctggat
     1801 tgatgtgacc ccaaaagttg aagttttaaa tagtggtgat taagcatgtg attgagtggc
     1861 ttgtttccgg taattaactg gagataggcc gctcaatttt acttttattc gatcgttatt
     1921 gtagaaatga atgtagtttt cgattctgca cttgaggtca ttaaaagaca ctttctcttg
     1981 accatcatac atttcttgct ttaagagccc aaagaaattc tccatcggtg aattatcaag
     2041 cagtttcctt tccgagacaa gctttgataa atgccgtgct cctttaaata actaatccat
     2101 gcacgatgtt gataatgcca cccctgatca ctgtggatcg ttgtacgata aggggcatgg
     2161 cgttcaatac gagaaattgc ctctgttagc ggctctaggg caatatctaa tgttggacgt
     2221 ttcgcgatct taaaggcaat aatttcgtta ttatataaat ccattatcgg acttaggtat
     2281 agtttttcat ccttgagaca cttaaattct gtgacatcag tgaccagttt ttgtagtgga
     2341 acggatgtca tgaatcgtcg atttacccga ttgctgacta ccttccctac tttcccttta
     2401 taggaattat aacgacgact tttatggaaa aatttcatac atttgatccc cagcttctgc
     2461 ataagacgtt ggactcgttt atgatttaca cagtacccca tgtttcttaa ggtgagtgtt
     2521 attcgtcgat agccgtatgt ctcgtttgaa tcgttaaaaa ttttttgaat aagctgttca
     2581 agcggatgat cttttgggtc atttgctttt tgggccatat aatagtgata tgttgatgcc
     2641 agtaaatcaa taaccattaa gacatatttt aagcgaaagt ggtcttcagt tagctcaaag
     2701 gctatccttg attgttgtct tcgcgagggt cgctcaatcc ccgctctctc aactttttta
     2761 aataggctaa ctcaatcttt aataaattat tttcttctcg taattgtgca ttctcacgag
     2821 ctacttgatc tgttttttgc tttgactgtt ttgttttctg ggtagatgag cgtgatgatt
     2881 taggcatttt gggtggcccc cctatcggct tgtttagact ttgtacgcct ccttccataa
     2941 atagccgttt ccaattcgct ataatagagg gttcgcctat gccaaaggcg ttagccatct
     3001 ctttatagga agcgccagtc tctagcttaa attttaatac attaagcttg aaacgtaacc
     3061 tatatttctt ctttgttttc tttcgtttta gcctagcttc cccataaagt tgatactgat
     3121 taacccagtt ttgaatgagt tgttcactgg atacatcata ttttttagca agcagatgat
     3181 agcctaatag accatcaaga tatgcttgga caacatttaa tttaaacggg tatgaatatg
     3241 ttttggacat aaatgaaccc ccgaaaatta gaataattga ctctaacttt tgggggtcag
     3301 tacaagaatg tcactaaatc taaatattaa atctcatatg aatttttgta gtttaataaa
     3361 ttttgatttt cttcgaagtt ttggacatca ctagatttat gattactatt ttctctaaag
     3421 aaataggatg taggaacttg taatctattg cttaactgct gtataatttt gctgctgggt
     3481 ttgattttgc cttgttcaat cttactaatt aatgcttggg tacacaatcc ttcagctagt
     3541 tctctttgac ttaaatttag atattctctt ctatctttaa ttttttgtcc aatcaaaatt
     3601 atcacagtcc ttttcaaata taaatttata ataagtaagt atacaacatg ttagaataat
     3661 cgaagtattt ataaattaaa gtatataata ccaaggctat gcgcttttac aattgcttga
     3721 attgtagatt gagtatccaa ttttttatta attgaatata aatggtttct gattgtgcgt
     3781 gtcgtaatcc ctagatcaaa agcaatagat ttttgtgttt ctccatttgc taatttttct
     3841 aaaattaatt tttcttgaga agttaaaaca tgtctacagt cattaattat atacgaggga
     3901 gaactttgat tatgtatttt ctttaattga gtgattaatg tatcgatatc gatagtttca
     3961 ttgaaaaatg caaatgcacc aatatcataa gctatttgtt taaactctaa taaatcaaga
     4021 gcagacataa aacatagttt agaacgtgtt tcatctatga tttttttggc aagtttaaat
     4081 tcatttagag tttctaaatg aatcactaat aaaataatat caatatctag gagaattatt
     4141 ttgttaatta aatcgtgaga aggttcatga aaataaaacg tatctatagt attagcttgt
     4201 gttaaggctg taaaaataca cttcccagag aaacaatgtt ggtcaattat gagaactttc
     4261 atacataaga tcctccacac tctatatttt atgaatatat tataacatat tgtgaatttg
     4321 ttatcaacat ttcctatttt acttattgtt attcgtgtaa taaagtttta aaataacaat
     4381 taggagctcc tagaatatta aatatgagga ggaaaaaaat gaaacaatca gataaagata
     4441 cagtgcaagg gaataaagag catttagtag aaaagaattt taagatttat gatgaagtta
     4501 cagacgaaga tttacaagaa aatggtggag tagcttggac atcacttgtt ccaatttctt
     4561 tagcaatatg tccatcaaca aaatgtgcgt ctattacaag accatgtaat aaataggagg
     4621 tagaatcatg aataaagatt ttgataaaga attgttaaat atttcaggga acttgaaaga
     4681 ggagttatct gaagcagact tagagcagat ttctggagct ggtgtcggta aatggattcg
     4741 caaattgagt aaaggtaaca ctgggaaagt ctgtacttta acttgggaat gtggtatttg
     4801 tccttcccat acgtgtttct tataggttaa tgattttgaa atagaaatat aaattaagtt
     4861 gttctgcctc ttaaatgtaa atatttttct aatgagaaaa agaggcagga caacttaaac
     4921 aacatttaaa ctttaattta aggaggagtc aaaagaatga aaagcagttt tttggaagta
     4981 aaagaaagta caaaaaagct attatttcaa gatctcaaag ggagcaatat tgacgaaata
     5041 taccaagatt ttgatataaa ctctatcaat caaactagag aaagtatatt gttcatcttt
     5101 aatgaggagc agaaaaagaa aaatgaaaag caatttgaat acacggcata taaaaattta
     5161 gggaatttta gtacttattt ttgtcaatat tttttgactc aatttgattt gtattcaaat
     5221 gaatatatag aagaaataga agacattgaa tttttcaaaa aaaatcttac agatcaatta
     5281 agtaataaaa ttttaaaaat atcatatcgg acacttattt acgagatgaa tgtaatgcga
     5341 aaagagaata tgtttgaatc aactttaaaa gatgaacaat atcttgaatt ctgcaataag
     5401 ttaaaagata gtacgtttgt taaggagata ttattaaaat atcctgtgat ggattactta
     5461 gtgtatacaa ctattacctt ttttaaagaa tttatttttg aaatagtaaa acactttaaa
     5521 aatgataaat ctattttagt tcagaacaca ctaattattg aagaagataa gataaagtgt
     5581 atgattttgg gacaaggaga ctcacataat aaagggaaaa cagttgtaaa aataaaaatg
     5641 agtactggaa agacactcta ttataaacca agaaatttag aagtagatct attatttaat
     5701 aaagttattc aattactttc tgagaaggaa aatatagatt tatgtacgta tggcgtgtgt
     5761 agcttggaaa atcatggatg ggtggaagaa gtagagtata aaccagtcaa aacactggat
     5821 gaattattca tgttttataa aaatatgggc tctttactag ctatactata tgcactgaat
     5881 gctgtagata tacatattga taatatcatt gcaaatggag aaattcctat gttgattgat
     5941 tgtgaaagtt tatttcatag ttcagaatat catgaagaag aagataatgc tacaagatat
     6001 ttaaataact atttatcaca atcagtatta tctattggag tgctcccatt tatttttgga
     6061 actggatcaa cagatatcag tgccttatcg gatcaaaaaa ataaaaaact tcaattaaag
     6121 ttccaacaat aaagaatagt aacagatcga atatgaaatt agtaagagag tacgttacta
     6181 tggaagaaag taaaaataat cctaatgcaa atgatttaaa aattagtgag gaagaaaaag
     6241 agaaaaatat tttagaagga tttgaacttg gttaccatgc agtactaagc tgtagaaaac
     6301 agatactgca cttaattgac agaagtaacg ataagatgat attacgtcac attatcaagc
     6361 ccactttatt ttattcaaat ttaattgaaa taagcaatca cccttcaata ttaatagatt
     6421 ttgagctttt agaattatta tttgctagaa ttcaagatga agaacattct aaaaaaataa
     6481 atcaagaaga atttaaagat ttacttcaaa gagatgtgcc gtactttttc tcttgtttta
     6541 aagataaaga tttatataaa aatggagaag tgatagaaaa aaatagtttt actgaaagta
     6601 aatactcaat ttttataaga aagatgaaca tgttatctat agaagataaa gaaagacaga
     6661 tggacttaat taagtcgcga ttatctatta gtagatttaa tcaagatcag tataatctaa
     6721 aatcatttaa agaaaataac gctattgatt taaatactaa aaaacaattt attatagata
     6781 gagcaattaa aataggaaat gaagttataa atagtgctgt gaaatataac aacacatata
     6841 gttggctatc ttatcaatta attggcgaag tgggcgcccc ttctttttta tccattaatg
     6901 aaatggatta taatttatat gatggtttag caggacaagg aattatgttg cttgctttat
     6961 ttttacagac taatgataac aaatacaaaa aaatgttaaa tgccattata gatgatatct
     7021 tgaacaatgt acaaactcta aaaggctgtc ctttaggagc tttttcagga ggatatggaa
     7081 tcatatattt attagctttg tattgtaaga aagtggagga gagagacaca tatatagata
     7141 aacttttgtt tttaattgaa agtagtttag atgaattaaa aacagtagaa gagtatgaca
     7201 ttataagcgg ttttgcgggt atattattaa tactaacaaa tttgtattgc aataatataa
     7261 aatcgacttc tgtagttaaa gcaatggata tctgtgtaaa atatttgatt gatggagtaa
     7321 caaccgataa aaataaatat atcggatgga aacaaagcga aggaggagaa atacttactg
     7381 gattttcaca tggaatagcg ggaattacgt atagtttaca aaagtatcaa gaattaagta
     7441 tgaattcaat taatcaatca gaagtgatta ataaaattat aaaagaaact agagagtttc
     7501 aagcaaagtc taaaaacaat ggtaaattta ttgatttacg taaggagtca tataataaca
     7561 ttccctatgc gtggtgccat ggtttgccag gaattatctg ttctggtgaa caagactacc
     7621 aaacaaaaca aaacttgata gatatgacag aaaatgcctt tggaagaaat cattgtttgt
     7681 gtcatgggga tttaggaaat ctaattatat taagtgaatt caataaagat caaaattgga
     7741 tagtagatta tgcatatcat atttgtacta cagaaacatt attttctgga attggaattt
     7801 cagacctctc tagcgatgaa tttatggtag gaaattcggg aatatgttgg ggattgatat
     7861 acttatttaa taataaccta ccaaacgtat tgaaattaga aatttaacgt aagggaaatg
     7921 aaatatggat actccaatca ttttacaaaa aaataagtat gagtgtggct tggcagctct
     7981 tgcaatgttg tttagttaca aggataaaaa atagatatat catttttgaa aaaagataag
     8041 caagaaataa ttggaagaga cggattaagt ttagctgact taaaaaaaat tgccaaaacc
     8101 aatgcaggaa acctaagagt atttaaagta tatgatatca caaaatattt agacacttat
     8161 gaaattactc aaccgttgtt gtgttatata aataatagtc attatatagt ggttacatgt
     8221 aagaaaaaag atagaatatg ggtaaatgat cctcaagtag gcagatataa tatttcagaa
     8281 caagaatttt caaatctatt ttcaggctat atagccattt tagaaaaaaa taaaaaaaat
     8341 aaagatatat ttgaatttag ctcgaaaaag aatgctagat tattcagtta tataactact
     8401 cattcatctg tggtaataca gcttatcttt tggacgttgg tttttcaggt attaaatatc
     8461 ttatttccgg caatgataca gaaaattatt gatcaagaaa taaatataac aatttttagt
     8521 ttatttatga tactaggatt atcgatagtc tattatctta tatcaaaata tcaattgaaa
     8581 ttggcaacaa aattacaaat tattatcaat aagttattga cgatagattt tgtaaaaaaa
     8641 gtattctcga taaaaagttc atttattgaa ataaattcaa ttgggtattt tactaatagt
     8701 ctttctaata taaatactat aaaagaaatt ttggttagtt tgtctacact tattgtaatt
     8761 aacgttactt taatgttgat ttacacaata gcattaattt acttttcgcc aatattaaca
     8821 ctgatagtat acggatttgt aattatgcaa ggaattgttt tatggaaagc tgctcccaaa
     8881 ataagagatt ttataggagg ggaaataaaa aataatattg atttccagaa ttactatata
     8941 gaatcgttaa aaaattataa caacataaaa tcaatgggag acccaaagta tgtagttaac
     9001 tttattcttg ataagtttac tggtcaattg cattcttttg aaaaaagaat ggatattcaa
     9061 attaaattaa cttcattttt aggcacggtg caatggttat taccatttat agtgttaata
     9121 gctggctatt tacttatgga ttctattaac ttaacaatgg ggtctttagt agctataagt
     9181 gccatagcat taagaatagt tacaccgtta gcatcagctt ctgaagtcta tcaatcaatt
     9241 ttaatgatga aagaaatttt tgaaaatatc gataatgtgt taactcaacc tgaagaaaat
     9301 gctgataaag gcaacagtat tgatactatg ccttttccaa ttaaatttaa tgatattggt
     9361 ttttcttatg ggaatactga gatattcaaa aatataacat taaatattga aagaaatgat
     9421 aaaattatta taaaagggaa atctggagaa gggaaaacta cgctactaaa attactaact
     9481 actttatatg ctcctacagt agggaagatt tattttgggg gtaaagaatt aaagaactat
     9541 aaattaaatc acataagaag taaaattgga tatatgagtc aagattattc tctgatatct
     9601 ggaactataa tggacaatat aactatgttt gatgacagaa ttagtttaaa agatgtggaa
     9661 aaagcagccc agttagtttg tatagatgaa tttataaatc aaatgccaat gaattactac
     9721 actttttgaa ttcgaatggt tcttcgctgt caggaggaca aatccaaaga atttgtcttg
     9781 ctagagcaat tgttaggaat cctgaattat tagttattga tgaaggtaca agtaatttag
     9841 ataaggactt agaaagaaaa gttatacgca acctttttaa tttgaactgt tgcatttgta
     9901 ttgtaagtca tcggcataac gagattgatg gaatcaataa agaaattgtt attaataata
     9961 attctttact aattaaaaaa tataataaag gggatataaa ttgaacacaa ctaataaact
    10021 aacaagtctt tttataaagg aaaattattt aaatagtatt gatgacaata ttcaagttat
    10081 tatagaagga gagcagacat ttttaaataa ggacaagatt tcaaaaacat tgtatgaata
    10141 cttctataat acaatatatg aagtgtctat caaacagtta ttaaaatggt tccattcctc
    10201 ttttgattat atagatgaaa cagataaaca atataatata aataaattta taagttttaa
    10261 accattttat tctacaagag aatttctaat attagataaa ttgatagtga aaattataga
    10321 aaataaaaca aattttgcaa aggaagtgat aagtaatttt cagaagagtt atgagaatat
    10381 aaaatctaaa tatagacttg gggaatctat aaatttatgt atagaagatt taatatttga
    10441 agtaggagat ttacacaata gtaataaaag tagctgtgtt atcataacta atcagaaatt
    10501 aaaactagtc tataaaccaa gaacagcagc caatgataaa ttcttttatg aaatagtaaa
    10561 ttttcttgaa aaccatctaa atttggttca aacagaatat ccatcactaa taatctcgga
    10621 agctacattt tctatttttc cttttattga taaacaagaa ttaactgata atgctgaagc
    10681 tgaggaatac tattatcgac taggcatttc aactatggtt ctttatgtag ttggagcaac
    10741 agatatccat tttgagaata ttatgtcgaa taagcaattt cctatattca ttgatttaga
    10801 aacattattt gatgttacta aaataaaaat acaggaagag agtaatgaat ttaacagcga
    10861 actactccag tctgtgttga caagtcatat atttgattat tcattaaatc ctaatcaaac
    10921 cgatatagta gggattgggg gctatacgac atctgttaat ttggaatcag aagaaaagct
    10981 tattaataaa gatacagatg aaatgcattt atcttttata cagactaaaa aagatatatt
    11041 taatgtacct aggctagcta attgttttct tgagataaca gaaaagaaga cacaatttat
    11101 aaaagggttc tacgatgctt acaatattat tttaagacat aaaaactatt tgaaaaaaat
    11161 aataaaagac tatagttcat ctataatttt acgtacgttg atcagaccaa catatattta
    11221 ccataattat cgaagaaaaa tattagaaga tcttactatt atccacaaaa atgaaagatt
    11281 atggaaaaaa ttagagcaaa attctatggt aaataaagaa attataaatc tagaaaaaag
    11341 agcattaaca agatatgata ttccattttt ttattgtata atgaatgggt gttcactttg
    11401 ttcagagggc gctcgagttg aaggattttt acctattact cctttagaat actatttgaa
    11461 taaactagaa aaaataacca ctgaagattg tttagttcaa ttatggtatt taaatcaaag
    11521 tattgaaata tataacaact atttatttaa aaaaataaat actgaaaata agaatgatat
    11581 aaaaatgtat ttggaaagaa gaataggtgc acacctagct gaagatgttt cgttttgctc
    11641 aataaagatg tgttataata gtgacatcgt tttatctccg atgaattatg atctatatga
    11701 tggtttgtat ggcttgaaat ttatggacaa aattttttca gagagataca atgtacattt
    11761 aattagagat aggaacaaaa ttcaaaatat tattgataag ttatctaaaa ttaatctagc
    11821 caatttttct gcatattcag gccaattttc acaattaaaa tataatttat taaaatttga
    11881 aatttctaac tcaaatattg attttaagaa tgattggata acatacagtc aattagtaga
    11941 gaaaataaat aaactaagta cccccaatat tgatttttta aatggtattt cgggaataat
    12001 ctcacttatg atagaacttt atcataagac taaagatgaa agaatcatta ttgatataga
    12061 aagaacttca aacttcttgt gggaggagta tcaagagtct ccaatccaaa actcaattat
    12121 tagttttgct catggtgcaa ttggaataga gtatgttttt tctaaagtgg ttcctttatt
    12181 gtctgatgat tcaaaaatta aacagaaatt aattagacat ctggatgaaa agttgactga
    12241 aataagaaat tatattcagc atgttattat aaatgaaact gaaataaatt acacatggtg
    12301 taatggatta acaggtttcc tggttgcgtg gctcgctata aacaaacact taaattgtca
    12361 atttagtgaa ttagagaaaa agttaattaa taaatatata gataattata cttttaagat
    12421 gttagatgat tctatttgtc atggacatgc aggaaactta ttgtctttaa attatatttt
    12481 agatagaaat ttaaataaag gctataggag aggagatctt attgatttaa aaattcagat
    12541 agaacgttgt ttacagaata agataatgta taataaaatt tgttctggtt atgaatataa
    12601 agatataaaa agtttcagtt tatttttggg agatttagga gtatatgcga gttatatctt
    12661 gagtactgaa ggaatctcct cgtcaatact ttactaactg caaggaggat aaaagatgtt
    12721 aagtatgcaa tcaatttcga aaaaatatgg agagctatat gcgctaaaag atgcggaatt
    12781 agagataaag aaaggtgaga tggtcgcatt aattggaaat aatggatccg gaaaaacgac
    12841 attgatgaaa attattagtg gtattaccaa agcgagtacc ggaacgtttg agtgggagac
    12901 caatcgtcaa actaatttga ttgaaacacc ggagttcttt gattatctat ctggatataa
    12961 gaatttagaa tattttcgca ttcaaagagg gattcaagat caacatatta tcgaagatat
    13021 actggaaaga gtcggtttac cgaatgaccg taaaaaattt aaacagtatt cattagggat
    13081 gaagcaacga ttagctatag ggttatgttt gttagggaat cctgaattta tggttttgga
    13141 tgagcccaca aatggagtgg atgctcaggg gattatcgat attcgaaatt tactcttaac
    13201 tttaaataaa gaagagcaat tgacgatgtt gattagtagc cataatataa atgaacttta
    13261 caatatcgcc actcgatttg tatttatcaa aagtggagag atcaaaaaag atatcagtaa
    13321 atcagaatta aatgaaaata tcggaacatt ttaccaagtg aatatcggcc ggatacaaga
    13381 tatagaagcg ctaataaatg acacggtatt acagtcgaat tattatacaa tcgacgacaa
    13441 gaatacgcta aaattggtta aaaattttga aacaagtcaa tttattgaac aattgtctca
    13501 aaaagagctc attgacgata tatctgaagt gcaagtgaca ctagaagact attatctaga
    13561 aataatgaaa gggtagaaag cttatggtga aaaaatatat ctattcagaa ctataccgaa
    13621 tcaaacatat tcgaatgtac atcgcattgt ttttcataac aattttattt ccgatagcta
    13681 caacagcttt tctttcgata atttctctcc agcagacaga gcctactacg aaagaatttg
    13741 ggttgtattc attgtatatg ttaatattta ttggtcttta cttagcctat ccgattgtta
    13801 tttgtgcgaa cacagtgaaa cctaaatacg ttgaacgaca aattttgtca tcagggatta
    13861 caaaaaacat tctatatttt tctgatttat tttatattaa cattatccaa ctaattatgt
    13921 tagtattgta ttcggtgtct agtatcatat cgactacttt gttgttagaa agcgctccag
    13981 gggcagaaga ctcactggat ctagttaccg tgaaagagtt tataataata atgtgttgta
    14041 tctatataac catgatttta tccagtattg tattatatag tttgcagcta gtgttgagca
    14101 ataaaatttt tgcgacagga ttatttatgt ttttagtcta cgtgattccg ttggttattg
    14161 cacaaatcag atatttctcg gatatagcgg attcagtcgc tcaaataatt ccgtttttac
    14221 aagttgcctt tttctataaa ggggatagtc cattaagtat gttattgctt attcatggta
    14281 tttggatagc agtgaccatt atgatatgta acacgattta taacaaaata gaagcttaaa
    14341 aataataaga aggagttgtt gaaaaatgaa agaatcatta ttttttgtgt cactattgtt
    14401 tttagcagtg tttttaatct taggagttta ttggctaacc cagcgatggg tatacgatgt
    14461 tgatgttagg ggattgcgta aatggatttt aacgtccttt gtattgtacg ctattactta
    14521 tggaattagc ttgtatttaa ttaattaaaa aataattcta caattttggg tgttggtgag
    14581 gatttttctc gccagcacct tatacaattt tacctgtaga ggtgattagt ggagatgaga
    14641 cttcaatttt aatgatatac tctgttgctg taacatttgt gctaaaaaaa tggtagatct
    14701 tcagtggtcc ataggtttac gtcgatatca atctgctaat taattttata tgttatcatt
    14761 ttcaaatata tgatatgcta tttttataag tgtttaatgc tagaagtcca ataagatttt
    14821 tacattttta tatcaatgtg gtacaaagaa gcatacgggt tatacactca tatacttaca
    14881 ctacgagagg aatagatgcc agcgttctca ggaacggtgg cctatttggt gatgaaagtc
    14941 cactatgagc ctttacagta ggaattatta gccaaaggca agggtgtcta tcgtgagatg
    15001 gaatctgaag gaagccagag gcagacacct gcactgacga acagaaatct cataaagaag
    15061 accggatagg tgtggctaaa cttgtcggtg ataagggatt aatgaaaaaa ttaggattag
    15121 gaactgaagc aactcgatct ggaataattg aaactataaa atatcggaaa tatattgaaa
    15181 atgatgggaa gacaaaacta aagccgacta aaaaaggatt actactatat aagataacta
    15241 aatatgtttt agtgagtagt cctgaaatga ctacaaaatg ggaagcttat cttgaacaga
    15301 ttggtcaagg aaataaatca cctgatccat tcattaaacg cgtaaaagaa agcattaagc
    15361 caacgttcgc tgagcttaat gataatgaag caggtacaga atcggtacag tcagttaaaa
    15421 cacaaaattt agaagaaata ggcaattatc aagttaggac taagaataag ttgtttgagg
    15481 taacgccaga gggagcaagt gagcattttc ctttatggag aaatattgca gggcatatat
    15541 gagtaaatca gatatgcgtg acttattgat gaaagggaag actagtaaga aaaagaaatc
    15601 taaaaaggaa atgagtttaa agcgtactgg gtgctgaaaa atcagagggt agaatttgag
    15661 ttttgattga taactttata caaaaaggaa aaatgtcccg aatattatac tgaaatccga
    15721 gacaagtaat tttgcattca ttttgtattg aggacaattg aaatgggcta tagacactgt
    15781 tgtgacaagg attgtaagaa gggagtttaa atcttcttat gcgcattaca tattaagttg
    15841 caatatctca ttgcacaccg aaccccggaa gagtgattca ctttttcgag gtttttttga
    15901 ttacttatta aaaaatagcc gagaaacgct agtaagatag acgtttctcg gctattggtg
    15961 tttcaattag ttaaaccgct tagttaccgc cagcatattt gaactgaggg tctgttaatt
    16021 tgaattcgaa atcatctaag tcaacgattc ccaccacttt atcggattcg agcaactccc
    16081 aggcaaattc tgccatctgt tcactggtgt ggtagcgttc gaagacatca gagtaatcaa
    16141 catctgcttc agcatcacca cgaacaacgc cgacaaattc ggtctcagtt gcagcaggag
    16201 caagtacttt agcccgagct ggtttgtttt tatttgctaa ttcgtgggct aagttttcag
    16261 ttaaggtact gacaaagtgc ttagacacgg aatagagtgg ggcaccttcc attaaagcat
    16321 agccggccac agatgagaca ttgagaagtt ggacaggttc atccgcataa tcttgcaagt
    16381 aaagaattga caacttagct agagcccccg tattgagttt taacattttt aagatatcat
    16441 tgggatccat ttccaagaag tcaccggata cactgattcc ggcattgtta acccagagat
    16501 caatgttgaa tgcttttgtt ttttcataaa aatcatctaa gcgggccaca tcaccaatat
    16561 cctgcacgag gtactcggca cttacgtggt agttttcttc gatatcgaca gcaatttctt
    16621 ttaaccgttg ttcacggcgg gccacgagga taacattgtg tccttcacta gctagtttgt
    16681 atgcgagatc acgcccgata ccagagcttg ctccagtaat taaactatat gtttttgtca
    16741 tgagttatca acctttctat ttgttactta gtataccata gataataatg ttaggaggga
    16801 acggtgcttt attgcgtgac ggtttcagat tcatcagcgg tagattgtgc tgtatcatta
    16861 atttcaacag ctgctgtaga gctgtttcgc aagaggcgca ggccatttaa gataacgaga
    16921 atagtcgata cttcatgaaa aacaacgccc atcggtaaat cgagccagcc taaaatattc
    16981 agtgtgatga gtgtgataat cacaccaatc gcaaagaaaa ggttttgctt aataatctta
    17041 tctaggcgtt gacttaagcg ataactattg aacaattttt gtagatcatt cttaacaatc
    17101 acaatatcag cagattccat ggccgcttgt gaaccgctgc ccatcgcgat tccaatatcg
    17161 gcatgagcta aggctggagc atcgttaata ccatcgccga tcatgcccac cacttcattg
    17221 ctagactggc tggattggat gaaattcatt ttatcttctg gaagcaattc agcataggtt
    17281 ttaggaatac ctatatcggt agcgactgct tcggctacgc gttgttgatc accagtgagt
    17341 aagactactt taacgccagc tgcttggaac tgttggaccg cttgctgaga gttgacgcga
    17401 attttatcgg ctaaattaac ataaccgacg atgcgatcat gttcagctat aataacggtc
    17461 gtatctgtac tgtcgatagc tgcttggacg gtttcaattc ctgttgattg tttgaataaa
    17521 tcaggtttgc cgactgtgat attgcccatt cttaagccag ctcccgcaat ttcttcgatt
    17581 tgatccgatc cagttggcaa gtcgatgtgt tctgattgga agtgagcaac aattgcttga
    17641 gcaattgggt gattagattg ttgttccata tagacaagcg ctgaaatata atcatcggct
    17701 aactcataat cagtgacact gaattctcct tgagtgagtg taccagtttt gtcggtatag
    17761 agtgttttca attcaccgaa gcgttccata accttacccc ctttgaagag gataccgttc
    17821 tttgcgccgt tactaatagc gctgagtgtg gctggagtga tagacgcaac taaggcacaa
    17881 gggcttgcta ctgttaagag aacgagtcca cgatagaagg cgtcttgcca actgagctct
    17941 tgcagaaaaa ataatccaat aataaataga ggaactgcaa tcagaacacc tgtgacataa
    18001 taggattcaa atgtatcaat ccagcgagct tttttggagg gttgctgttg tgcttgttcg
    18061 accatatgaa taatgttgga gaagatagtg tcattacttg tctgagtaac tgttagataa
    18121 aagacatctc cttcattcaa tgttccggca tagaccatat caccgctaga tttttctact
    18181 ggaatagatt caccagtaag ggcggattcg ttcaatacag cttggcgatc gatcgtgcca
    18241 tcaatcggaa gttgggctcc tttttgaacg atcactgtgt caccgattgc taaggaatca
    18301 gtgggaacac gatcaacctt gccgttctcg cgcataatat gagcttgatt aggaacgcta
    18361 gacatcaact cagagatgct gtcagttgcc ttatctgtta cgtaatcttc taacacttct
    18421 gcaccagcaa aaataagaag taatatagcg ccttctgatt cgtattggat tagggtggct
    18481 cctaaggctg agagaatcat cagcagagca tcattggggg ctttatgttt aaaagtttca
    18541 actagcgctt cttttgctgc atagaagcct aaaaatacaa tagcaccata gaatgcccac
    18601 cgactactcg ttaaatccat tattgatagg ctgaatccta gtactgttaa tagaatacct
    18661 atgactaaaa attgtccttc acgatttgtt tttaaataat ttaacattgt aatctctcct
    18721 ttggtaaaaa ataacttgaa taatgagtaa ataaactgcg ctagtgaaat gatcctcctt
    18781 ttttagctac tgtactaaaa aaggcacacc tgtggacaga attaactgtc tcacagatgt
    18841 gccttaacac tctgctgcta cgtttagtag ccgggctgca tgaatagtca cctattcacc
    18901 gcttgctcag atttgtgctc cttttcagta gaagcgctat gtaattttta ataactcatc
    18961 taacttattt atagtatact acatggtaat aagattgtaa agtatttcac gaatattttt
    19021 caattttttt gatagtaaat agtaacgagt gatggaaaga ataacgggtt ctcactgtgt
    19081 ttaaagacga aattgttttt ataatatgcc ttattatact tttgtatatg gaattttatt
    19141 aaatgttagg tttattgata attttttatc ttttttattg taatggttat tattttttgg
    19201 tagattatag gcaagaaatc ggtataaaaa ttattctaca ttggagtgat tataattatg
    19261 actattcata tcaagatgcc aaaattactt cccaacatgg aaatgggaaa acttgttgct
    19321 tgggttgtcg atgaaggtga aatattcgaa gctggcgatc cgttatacga agttgaaaca
    19381 gataaagtcg ttaatattat tgaggcagag atggatggct tgatgatgaa gcatcgttac
    19441 gaagaaggag atgatgtacc cgttggagca attgttgctg aggtggggcc acgctcgtca
    19501 aagcctgatt ggttgcgcgt gtcttacaat gggagtgcga ttaaaaacgt ctctaatatt
    19561 ttagaagaga atagattaac aacagtttgt gaggaagcaa attgtccgaa tatcggggaa
    19621 tgtttcggca ataacaccgc aacctttatg attatgggag aaaaatgtac ccgtggctgt
    19681 aagttttgta atattgaacg gaccgctcgc ccgggccagc ttgatccact tgagccaatt
    19741 cgagtggccg atgcagtccg taagatgaag cttgagcatg ctgtggttac tcaagtcgca
    19801 cgagatgatc ttaaagatgg tggtgcagcg catatggctg agaccgttcg ccaaatccgc
    19861 gagatcaatc cggagacaac aatcgaagtc ttgatctcag acttacgcgg ctcttatgaa
    19921 gcattggaag atgttctcgc agccgatccc gatgtcttga accacaacat ggagatgcca
    19981 aaagaatggt accgaaaagt ccagccacaa tgtaattacg aacagtcctt gaatgtctta
    20041 cgcaacagta agaagatttc accgaatacc ttaacgaaga ctggattcat ggtcggtcta
    20101 ggggagtctg atgaagagat ctctaacttg atggatgata tcttagcgac cggttgtgat
    20161 attttgacga ttaaccaata cttgcaacca tctagtgagc accataagtt ggcacgctac
    20221 gtgacaccgg agaagttcaa tgaatatgaa gaaatggccc gcgaaaaagg atttaaatat
    20281 gtcgtagcag caccgctcgt tcgttcatca tacaaagcag cggaggcctt cgaacacgct
    20341 aaagcaaagc aggcttagcc tatgttgttt attaataatg attctaatga cgtatatttt
    20401 aattttgctt tagagcatta ttttgtaacg gaaaaacaaa tgaatgagcc cttattagtt
    20461 ttttggcgaa cggagccaac gttgatggtt gggaagtatc aaaatattta tgaagaagtg
    20521 gatgtcaact atgcaaaaca gcacgatatt aacattgtgc ggcgtccatc tggtgggggg
    20581 actatttata cagatttagg cagttggcaa ttcaccttta ttcattatca gacgaagcga
    20641 gagatattct ttgatgattt cacgcaaccg gttattgaag cactcaatcg tcttggcgtg
    20701 ccggctgaat tcaatggtcg gaatgacatt gtgattgaga acaaaaaaat atcgggcaat
    20761 gcacagtaca aagaaaaagg gattacggtc catcatggct cattgctctt cgatacaaac
    20821 attgaggaaa tggtgcgcag tacgacggtg gatgaacaca aaatcatctc aaaaagcatc
    20881 aaatctattc gcgatcgggt gaccaatatt gctgagtatt tgtctaatga ctttacagca
    20941 atggacttca agcagtatat ggtccaagaa ttgatgggta atgaagcaac aatctatcac
    21001 ttaacgagaa ctgatattgc tcgcattaat gaactggcgg atcaattatt tcgcagtttg
    21061 gaagcgaaat ataagcggaa ccccacattt acacgtacaa caacgcatta tttggcggga
    21121 ggtaagctca gtgtgtcgct caatatcaag tcgggacgta ttgctgaatt gcaattgaca
    21181 ggggattttt tctcaggtga agatattgaa gaattttgtc agaaacttgt tgggacacgc
    21241 ttagacgaac aagcgattcg agaaaaaata acagaattaa ctgatcagtc gttgatttac
    21301 aatatctcga atgaagaaat cgccacagcc atcttagata tcccacaacg caatatagat
    21361 taaggtaggt gcacagatat gatggaaaac ttaaatgttg aattgttgaa tgaagcccaa
    21421 acgcatcaag aggtcttaca ccagcaactc acgcaagtca ttgatcccga gttaggtatt
    21481 gatgtgttga atttaggctt gatttacaaa cttgatttaa ataaaaaagg ggactgtaaa
    21541 gtaaccatga cgcttacatc aatgggctgt ccgctagctg atgagattat gaatggtgtc
    21601 cgttttgctc tgcttgattg tgatcctgtt gaatttgtta atattgatct ggttttttca
    21661 ccggcatgga gcacgagcca tatgtcgcgc tatgcgagga tttctctcgg cgttagagca
    21721 taaaaaataa atagagaaaa taaaagaagc tccccgcaaa ttggggagct tcttcaagta
    21781 gaaaggagat gataaatgaa aagaaaaaca aaaagctgtt tgtcccatcc atctatttat
    21841 atgataccgc attctggaaa cggtgtcaag tggaaatgga aattatttat ttttttagta
    21901 aggaaatcat gaactaccta taattcagta accaatcgta atgaatgagg ctatgtattt
    21961 tgaatattaa ttgtcttagt aagaagacat atattgtgcc tttttttaca atggattaaa
    22021 cttgtggtat gataaagatg taaacagata catggatgac tataaaaaat actgagagaa
    22081 taagaaaggg agtcgggtaa aatgaagaaa atggttatat ttgatcctgc tatgtgctgt
    22141 tctacaggag tttgtgggcc ttctgttgat gaaaatttgc tgagggtatc tacagtaatc
    22201 aataaattaa aaaagaaaga tgtagtagtt gaaagacata atctagccaa tgatccaaaa
    22261 gcatttgtgg agaatgaatt aattaaccaa ataattgaca gtgaaggaac agaggcactg
    22321 ccgattacaa cagtagatgg agaagtcgtg aagaagaaag aataccctac aaatgatgaa
    22381 ttttgctcat ttttagatat tcctaaagca tatctggatt catcgaaaga acaatcaaca
    22441 aaaaaatgtt gttgtggaaa aggtaattgt tgctaaaagg gagtgaggtc atatgtttga
    22501 atcatttaat gcagataata ttgatttaac taaatattta ttcttcacag gaaaaggtgg
    22561 tgtcggtaaa acatcaactg cttgtgccgt ggcagttgca ctcgcagatc aaggtaaaaa
    22621 aattatgttg gtcagcacgg atccagcctc taatttacaa gatgtgttcg atacagaatt
    22681 aaataataaa ggggtct
//
