LOCUS       NZ_CP040419            12154 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3069 chromosome, complete genome.
ACCESSION   NZ_CP040419
VERSION     NZ_CP040419.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977296)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 372x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655825.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:32:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,913
            CDSs (total)                      :: 1,844
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 6 of 44
            Pseudo Genes (multiple problems)  :: 5 of 44
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:54:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 119265
            Orig. end                         :: 131419
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040419.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(807..1688)
                     /locus_tag="FE326_RS00620"
                     /old_locus_tag="FE326_00605"
     CDS             complement(807..1688)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635130.1"
                     /locus_tag="FE326_RS00620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00605"
                     /product="homoserine O-succinyltransferase"
                     /protein_id="WP_208954324.1"
                     /transl_table=11
                     /translation="MPIVCDQNRYGSDVSMISNAEIQMNRTHPIKIAILNLMPNKITTE
                     HQLLRLLGKSPLPIEVTLLYTKTYAPTHMIDDYLQKQYRVFDDVKDDTFDAFIITGAPV
                     EHLSFEQVDYWNELTDIFTYVHSNVRSTLFLCWASQAALHFYYDIPRFEVSEKIFGIER
                     FKTRVKNSLTDGFESYFNVPQSRYFYHQLSDIQSHSDLILYAGSEQSGAQIVASKDYRS
                     VFIAGHFEYDADTLHLEYKRDQERGLHTSLPTCYFVNDDPTQGYCSTWQNHAERFFTNW
                     IKHIVDPTVSDA"
     gene            complement(1723..2193)
                     /locus_tag="FE326_RS00625"
                     /old_locus_tag="FE326_00610"
     CDS             complement(1723..2193)
                     /EC_number="4.4.1.21"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648167.1"
                     /locus_tag="FE326_RS00625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00610"
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="WP_208954325.1"
                     /transl_table=11
                     /translation="MEKIASFTVDHLNLKPGLYLSRTDQIGERKLYSYDLRFTAPNYEP
                     VMNTAEIHTIEHIVATYLRNDDQFKQEIIYFGPMGCRTGFYLITTDAIAPADLLDLLIR
                     AFQFTASFQGEIPGADARSCGNYLDQNLPMAKYYAKHYVTVLENVTDLTFSY"
     misc_binding    complement(2265..2512)
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
     gene            complement(2713..3468)
                     /locus_tag="FE326_RS00630"
                     /old_locus_tag="FE326_00615"
     CDS             complement(2713..3468)
                     /EC_number="1.1.1.100"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (4-126). E-value: 5.7e-08. Score:
                     24.8. Matches aSDomain:
                     nrpspksdomains_FE326_RS00630_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF006393.0"
                     /locus_tag="FE326_RS00630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00615"
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="WP_111951094.1"
                     /sec_met_domain="adh_short_C2 (E-value: 3.9e-37, bitscore:
                     125.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKVVIVTGSSRGLGKAIAQKFADQGAYIVINYVKSEDKAQQLV
                     QDIGEDRAIAIQADVRDSEQINHLVTKTIEHFGQLDVIVSNALINFKFDPHAQQSLDTI
                     DWKNYNEQFEGSVKAALNLVQASLPYLKDSPQARIITIGTNLFQNPVVPYHEYTSGKAA
                     LLGFTRNIAKELGPDGITANMISGGLLQATDASSVTTPEVFDLIANSSALQRVTTPEDV
                     AEVVAFIGSEASRGLTGQNITVDSGFTMN"
     aSDomain        complement(3091..3456)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FE326_RS00630_PKS_KR.1"
                     /evalue="5.70E-08"
                     /label="FE326_RS00630_PKS_KR.1"
                     /locus_tag="FE326_RS00630"
                     /protein_end="126"
                     /protein_start="4"
                     /score="24.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVIVTGSSRGLGKAIAQKFADQGAYIVINYVKSEDKAQQLVQDIG
                     EDRAIAIQADVRDSEQINHLVTKTIEHFGQLDVIVSNALINFKFDPHAQQSLDTIDWKN
                     YNEQFEGSVKAALNLVQA"
     CDS_motif       complement(3385..3453)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FE326_RS00630_0001"
                     /evalue="1.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FE326_RS00630"
                     /protein_end="28"
                     /protein_start="5"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VIVTGSSRGLGKAIAQKFADQGA"
     gene            complement(3557..4126)
                     /locus_tag="FE326_RS00635"
                     /old_locus_tag="FE326_00620"
     CDS             complement(3557..4126)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Mac"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635128.1"
                     /locus_tag="FE326_RS00635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00620"
                     /product="sugar O-acetyltransferase"
                     /protein_id="WP_077863115.1"
                     /sec_met_domain="Mac (E-value: 8e-11, bitscore: 38.8,
                     seeds: 568, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKHEYNKMIRGELYDPRDPYLRDLMTKQHALMDAFNESGRTNHA
                     TQNKLLEKLLGQYHGESSHIRRPFFIDYGLNITIGKHFFANYNCTMLDVAPITIGKNVM
                     FGPNVSLNTPEHPLSAAERNMGLEFARPITIEDNIWIGASVTVVGGVTIGQGAVIAAGA
                     VVTKDVPANTVVGGVPARVLKTIENC"
     misc_feature    4104..4121
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.33"
                     /tool="antismash"
     gene            4348..4701
                     /locus_tag="FE326_RS00640"
                     /old_locus_tag="FE326_00625"
     CDS             4348..4701
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS00640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_00625"
                     /product="hypothetical protein"
                     /protein_id="WP_077863116.1"
                     /transl_table=11
                     /translation="MHTRFKEGLKSFIISYVAMILYHLPSAYNPLFDIVHMMVIVGTLL
                     YGGYYFYRNGASSNGMAGLLIQVSLFTIGFNIALPLTREIFSARIMFMTGCSLSLIFLK
                     HIENNLYRIDRKS"
     gene            5001..7154
                     /locus_tag="FE326_RS00645"
                     /old_locus_tag="FE326_00630"
     CDS             5001..7154
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /locus_tag="FE326_RS00645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00630"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_077863117.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.9e-37, bitscore:
                     124.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 2.7e-233, bitscore:
                     773.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTNFRVIYQQDNMDCGTACVQMILHHYNSEFSIHKLKDLTDKGK
                     QGTSALGIKRCLESLNFDCLVIDADNAVWTDSAMTYPAIAHLNVHNGKHYVVVYGYQDN
                     KLFIADPDDGKYNMSIETFSAVWSRTLFVPSPGRHYKPIKEKVQGLMSFVPKIIQYKKF
                     VVFSIIASLLIMGLSIGSSYYFQGIIDSIIPANNISMLNIISFGLMISYVLRVIFGYIR
                     NYLLFILGQKLSRDIILNYFKHVLKLPISFFASRQSGEIISRFLDASTIIDALANTSLT
                     LLLDSIMVVVVGIVLLLQHNVLFGVTLVTLPLYIGTIILFIIKLDKSNEEEMESAAVLN
                     SSIIESLNGIETIKSYNSEKVIYQRVVGEFEDLMIKTLTTMTLDNMQGAIKTGTQLVGN
                     ALVLWLGAYYVMSGEMSIGALITYNALLVFFTEPLESVINLQVQIQKAQVASRRMNDIL
                     SIEVEGNISNDTVEIEEMGEADLKFDDVYFSYGLGELILRGITCEIKVGDKIALVGESG
                     SGKSTLAKMLVRFYAPSMGNIYFGKHDINTIDVHILRHNIVYVPQDSFFLAGTIRDNLL
                     FGLDYEPTQNQIEQACEVACIKSFIESLPLQYHTIVEEAGSNLSKGQKQRLAIVRALLT
                     NSQVIIFDEATSGIDVFSEQRIMDNIIKMTERTIIFITHSLPAAKLCEKIMVMDQGDLV
                     EEGIHEVLRYAGGTYQKLWEVIS"
     gene            complement(7219..7974)
                     /locus_tag="FE326_RS00650"
                     /old_locus_tag="FE326_00635"
     CDS             complement(7219..7974)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="FE326_RS00650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00635"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_004635118.1"
                     /transl_table=11
                     /translation="MYPLIICEDSIGQLNAIKQIINLYTGFYESRFNVSLITSSPHEVL
                     DYLETYQPKKGVFLLDVHLNSTIDGLDIAEVIRKNDVNAKIVFITTDEHAAPLTFKRNL
                     EAMSFIEKPSNVEELRKNIFETLNIAYNRLTTSLDEKKQLFTFSRYGETVNIDFEKVLY
                     LQTSIEESHKLTLTTTLGEYEFSGSLIKCEENFPELFRISKSALINPGNVVSINYKNRD
                     IVLKNNHIIQYAFRRAKSIKEKFSNPNKN"
     gene            complement(7992..8288)
                     /locus_tag="FE326_RS00655"
                     /old_locus_tag="FE326_00640"
     CDS             complement(7992..8288)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="FE326_RS00655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00640"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_208954326.1"
                     /transl_table=11
                     /translation="MVGILLDNAQEYVTTIEEKHIDILITSHTKVMELVISNPYIPDHY
                     TLRNFKQEGFSLKGNDRGMGLSTITKIEKKHNNLTVLFEKNTIFSAKIITTKL"
     gene            complement(8299..9147)
                     /locus_tag="FE326_RS00660"
                     /old_locus_tag="FE326_00645"
     CDS             complement(8299..9147)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS00660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_00645"
                     /product="hypothetical protein"
                     /protein_id="WP_208954327.1"
                     /transl_table=11
                     /translation="MIIHDLFYATDIVALLYGLVLYGIFFSLKRINFEVLNSILLLVYT
                     KGIVEFLVALPINTLKYYNLHNHILVMITLKILTFIVSIAIYKKIYPYFTSNIDQVKAK
                     KIVTYSLLILNILFSSYFIFIQWFDLYRKLLYFTLTITIIFVIITVGFLYALHKYLQLK
                     FQHELSNQHLEALTKYNHIVEEETYKTKEFQHDYKNLMLSTTSFIENNDLAGLKEMMHS
                     LIDYSKDYFSHSTFQYKDIHNIKHPALKSIILAKLVTFTELGITCQFECRKPLDNIYRY
                     I"
     misc_feature    9885..9898
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.99"
                     /tool="antismash"
     gene            9948..10367
                     /locus_tag="FE326_RS00665"
                     /old_locus_tag="FE326_00650"
     CDS             9948..10367
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF038270.1"
                     /locus_tag="FE326_RS00665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00650"
                     /product="Msa family membrane protein"
                     /protein_id="WP_112791385.1"
                     /transl_table=11
                     /translation="MYRKILLSLISTVIIFGIGIYLNVSMSLLMFAVISYVLPLMGNIV
                     IDYYVQTENILIGSGIISTITTTGYAIFAILMENNINFDGFIRQHTYRSGNMTMGLEPG
                     LADMSQLIFVFALNLSVLYFIQYLSRGDFSHVRRK"
     gene            10351..11238
                     /locus_tag="FE326_RS00670"
                     /old_locus_tag="FE326_00655"
     CDS             10351..11238
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635115.1"
                     /locus_tag="FE326_RS00670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE326_00655"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_208954328.1"
                     /transl_table=11
                     /translation="MLDVSNVSKQYSGNDFYSLKDVSFTIEKGEIVGLIGKNGAGKTTL
                     LKLLAKSHFPTAGVIRYQGLDIFSKDNVLRPFGLMIETVYYSHLTVKQNIEFYLDIHDQ
                     SEYKKNIQSVLETVELWHKKDFKPDGFSFGMKQRLSLAMSLVTNPEFMILDEPFVGLDP
                     DGVNDLMRILKQWAADRGTSIIVSSHQLNELEAICDRYLYIEGGALKKQFGKAAVEVTV
                     IELVDALQDRDALFSAYEAIQAISEDGKQIEVDSNSAAFHQILGTITASHNIKKIFTKE
                     NALNQYFKGSRDHE"
     gene            11231..11995
                     /locus_tag="FE326_RS00675"
                     /old_locus_tag="FE326_00660"
     CDS             11231..11995
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE326_RS00675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE326_00660"
                     /product="hypothetical protein"
                     /protein_id="WP_208954329.1"
                     /transl_table=11
                     /translation="MSKLNQVVFHNTLRKKEVWVYLAFTLLPLLVFVTELTDTKFLRLT
                     GDFSEMNFLDFYGTTLGIIDGMILPTIVIAYIASTMFYGEINKGILFLYKDINRKKVMN
                     SKVFSLMKTYGLYLLLVMLSSLVIYYFFVIQMKGGTFSLLGSVAPVTYYSVLGVLGLFG
                     IDIVAILVAANLSVRFSTGVTVLGTIFFLFLMQAISRVDGLNYLSPMSYRELFNVGQMT
                     FSMSFILLVFLCVVYVVSAYTLLYRQFSKVEY"
ORIGIN
        1 gtggataata cgttcccatc accatatttg ttgaatccaa ttaagtttgg agcggatatt
       61 gttattcatt cggcgactaa gtttattggt ggtcatggga cctcaattgg tggtgttatt
      121 gttgagagcg gtcagttcga ctggacacaa aacaataaat tccctctctt atcagagccc
      181 gatgattcat accacggctt gatttttgct gaagcagtac ctgatgcagc tttcacgact
      241 aaaatccgag cgggccttct gcgtgatacg ggggcagcta tttctccgtt caatgcattc
      301 ttattattgc aaggcgtaga gtctctacat gttcgagtag aacgtcatgt ggagaatgcc
      361 gaagcagtag cgcaattctt agctaaccat gagaaggtag aatgggtgga ttatgcggga
      421 ttagcggata gcccttatta cgaactgaag caaaaatact tgccaaaagg agctggctcg
      481 gtcttcacgt tcggagtgaa aggtggctat gataaggcgg ttgaattcat cgaagcatta
      541 gaactcttct cactactcgc caatgttggt gatgcgaagt cattagttgt tcatccggct
      601 agtatgaccc acagccaatt aacagaggaa gaattaaaat caggaggcat ctatccagaa
      661 acaattcgcg tatcaattgg tatcgagaat gtggatgata ttattgatga tctaacgcaa
      721 gcacttgcga aaatttaaag taaataacta aaaaagacat ccagcatatg tagcggatgt
      781 ctttttagat tcatcgtttt ataagattaa gcatctgaaa cggttgggtc aacaatatgc
      841 ttgatccagt tagtgaagaa gcgttctgca tggttctgcc aagtggaaca gtatccttgt
      901 gtcggatcat cattgacgaa ataacaggta ggcaatgatg tgtgtaaacc acgctcctga
      961 tcgcgcttgt attcaaggtg caacgtatca gcatcgtatt caaaatgccc agcaataaag
     1021 acagatcggt agtccttgct ggcaacaatt tgggcacctg attgctcact gccggcgtat
     1081 aaaatcaaat ccgaatgact ctgaatatca cttaattgat gataaaaata acgggattga
     1141 ggcacattaa aataggactc aaatccatct gtcaagctat ttttcacacg tgtcttgaat
     1201 cgttcaatcc caaaaatctt ctccgatact tcaaagcgag gaatatcata atagaaatgt
     1261 aaggcggctt gactagccca gcataaaaag agcgtactgc ggacattcga gtgaacatat
     1321 gtgaagatat cagttaattc gttccaataa tccacttgtt caaatgataa atgttcaacc
     1381 ggtgcacctg taatgatgaa tgcatcgaat gtgtcatctt tcacatcatc gaagacgcga
     1441 tattgcttct gtaaataatc atctatcatg tgcgttgggg cgtatgtctt cgtatagagc
     1501 agagtaacct ctattggaag aggggatttt cctagtaatc gcagtaattg gtgttcagta
     1561 gtgattttat ttggcattaa gtttaagata gcaatcttaa tcggatgagt tcggttcatt
     1621 tgtatttctg cattagatat catagacacg tcggatccat aacgattctg gtcacaaaca
     1681 ataggcataa tcatccctcc atttccgtat attactacta tactagtaac taaatgtcag
     1741 atcagtgaca ttttctaaga cggtgacgta atgtttggca taatatttag ccattgggag
     1801 attctggtcg aggtaatttc cacagctacg tgcatcggct ccgggaattt caccttggaa
     1861 gctggcggtg aattggaatg cacggattaa taaatcgagt aaatcagcag gggcaatcgc
     1921 atcggtcgta attaagtaaa aacctgtgcg acatcccatt ggcccgaagt agataatctc
     1981 ttgtttgaac tgatcgtcat ttcgcagata cgtagctacg atatgttcaa ttgtatgaat
     2041 ttcagctgta ttcatgaccg gttcataatt tggtgcagtg aagcgcaaat catacgaata
     2101 taacttccgt tcaccaatct gatctgttcg tgataagtac aatcctggtt ttaagttcaa
     2161 gtgatccact gtaaagcttg caattttttc cattatttgt ctctccttta tatgtttgcc
     2221 tataaaaaaa tacccgttcc tttgccgact aaatgtgtag tcaacaaagg gacgagtatt
     2281 gttcgtggta ccacccttat tcgcctgagt ctttcaacgc agcgcctcat cagcacatac
     2341 ttcctcgcag aaatataagc ctagccatat aacggtcgca cccgtcgagc cagcgtgaac
     2401 cagctcaaat cctcggagct catcttcatt cgcttaacca ttttcccttc gcaccaaacg
     2461 ggaatctctg taaacagcgc tacaaactac tcttctcctg aaatgataat cttataatat
     2521 taattaatag tttacagata gttaaaggta atgtcaagta ggaaatttta tttttttaac
     2581 aagttagtta atattgagag gactatataa ggcttcttag gagacattat ctataagaag
     2641 ccctgtattt tggtaattta ggttaaataa gtacatattt ttccacaatt aatcaacatt
     2701 ttgtgtgctt aatcaattca ttgtaaaacc actatcaaca gtaatattct ggccagttaa
     2761 tcctctggaa gcttctgatc caataaatgc gacgacttct gctacatctt cgggagtagt
     2821 tacacgttgt aaggcagaag aattagcaat tagatcgaag acttctgggg ttgttacaga
     2881 actggcatct gttgcctgta agagtccacc tgatatcata ttagcggtaa taccatcagg
     2941 acccaattct tttgcaatat tacgggtgaa gcctaataat gctgctttgc cagaggtata
     3001 ctcgtgataa ggtactactg gattttggaa taagtttgtt ccaattgtaa taattcgagc
     3061 ttgtggacta tcttttaagt aagggagact agcttgtact aggttaagag cggctttaac
     3121 ggatccttcg aattgctcat tataattctt ccaatctatt gtatcaagtg attgttgagc
     3181 atgagggtca aacttaaaat taataagcgc attactgact ataacatcaa gttgaccgaa
     3241 atgttcgatt gttttagtga ctaggtggtt aatttgctca gagtctctaa catcagcttg
     3301 aatagcaatg gctcgatctt cacctatatc ttgaactaat tgctgggctt tatcttcact
     3361 tttgacataa ttaatgacta tataggcacc ttgatcagca aatttttgag cgattgcttt
     3421 tcctaatccc cgactagaac ctgtaacaat gactactttt ttctccatga tgattcctcc
     3481 ttaaaattat ttcctataaa gtatacgcta acatgtttgt tttttaaatt attagccctt
     3541 gaaataatga gtatttttag caattctcaa tggttttcag tacacgtgcg ggaacgccac
     3601 cgacaacggt attggcaggg acatctttag tgacaacagc tcccgcggca atgacggctc
     3661 cttgtccgat agtgacacca ccgacaacag tgacgctagc gccaatccag atattatctt
     3721 caatagtgat cggtcgggca aattctaatc ccatattacg ctcagctgcc gagagaggat
     3781 gttcaggagt gttcaagcta acgttgggcc cgaacatgac gtttttaccg atcgtaatcg
     3841 gggcgacatc cagcattgtg caattataat tagcaaagaa atgcttacca atcgtaatat
     3901 tgaggccata atcgataaaa aatggccgac gaatatgact gctttcgcca tgatattggc
     3961 ccagtaattt ctctagcaac ttattttgag tagcatgatt ggtgcggccc gattcgttga
     4021 aggcatccat taaggcatgt tgttttgtca tcaagtcgcg caagtacgga tcacgaggat
     4081 catatagttc gccacgaatc atcttgttat attcatgttt attcatcaaa tcgctcctta
     4141 ctttgtttat tataccggtc agaatatacc ttggtcaata ttaactatta agcgaattta
     4201 tcgcaggata aacaagaaaa aaatagtgta ttcagacgaa ttagagagca ggatggataa
     4261 aattatgatg ctagctaaat accggacgta tcctatttat gatatacttt gggtaactgt
     4321 agcaataaat tctggagggg gagtctaatg catacgcgat ttaaagaagg attaaagtca
     4381 ttcattattt catatgtagc aatgatactc tatcatcttc catctgctta taatccattg
     4441 ttcgatatag tgcacatgat ggtgattgta gggacgttgc tttatggagg ttattatttt
     4501 tatcgtaacg gtgcttcaag taatggcatg gccggcctgc ttattcaagt gagtctattt
     4561 acaatagggt tcaatatcgc cttgccactt actcgagaga tctttagtgc ccgcatcatg
     4621 tttatgactg gatgttcatt atcacttatc tttttaaaac atatagaaaa taatttatat
     4681 cgaattgacc ggaagagtta gtataatata taaaatatga aagaagctct agagcaatgc
     4741 agatatatgt tactgataat atccaaaatt ttattgggac ccttaaaatt gactttaaat
     4801 tctatatttc tacacagtaa tgatttgtga gtgtttaata tgtcttacgg gaatttttta
     4861 tttagttatt tgataagttg gtataatttc aaaagataac gataaggtag taaatttgta
     4921 cgtttgggga agaatactca aataaataaa ttagatgtca tactatagtt agtcagtgaa
     4981 gaaaatagga gacatatgaa atgaatacca actttagagt aatttatcaa caagataata
     5041 tggactgtgg gacagcctgt gtccagatga tcttgcatca ctataattct gaattctcta
     5101 ttcataaact aaaagatcta acagataaag ggaaacaagg cacttcggca ttaggtataa
     5161 agcggtgttt agaatcatta aattttgatt gtcttgttat tgatgcagat aatgcagtat
     5221 ggacagattc agcaatgaca tatccagcta ttgctcattt gaatgtacac aatgggaagc
     5281 attatgttgt tgtgtatggt tatcaagata ataagttatt tattgcagac ccagatgatg
     5341 ggaaatataa tatgtctata gaaacatttt ctgcagtatg gtcaagaaca ctattcgtac
     5401 cttcaccagg aaggcactat aaacctatca aagaaaaagt tcaaggattg atgtcatttg
     5461 ttcctaaaat aattcagtat aagaaatttg ttgttttttc gattattgca tcattgctta
     5521 taatgggatt aagtataggt agttcttatt attttcaagg aataattgat tcaattattc
     5581 cagctaataa tatttcaatg ttaaatatta tttcatttgg attgatgatt agctatgtgt
     5641 taagagtgat atttggatat attagaaatt atttgttgtt tattttagga caaaaattga
     5701 gtagggatat catattaaat tattttaaac acgtattaaa attacccata agtttttttg
     5761 cgtctagaca atctggggaa attatctcta gattcttaga tgctagtaca atcattgatg
     5821 cattagccaa tacatcctta acattacttt tggacagcat aatggtcgtt gtggttggca
     5881 tcgttctatt gcttcaacat aatgtattat ttggtgtaac tttagtaaca ttacctctgt
     5941 acattgggac aattatactg tttatcataa agttagataa gtcaaatgag gaagaaatgg
     6001 aatcagcggc agtactaaac tctagtatta ttgaaagttt aaatggcatt gaaacaataa
     6061 aatcatacaa cagtgagaaa gttatttatc aaagagttgt tggggaattc gaagatttaa
     6121 tgataaaaac attaacaaca atgacgttag ataatatgca gggcgctatt aaaaccggta
     6181 ctcaactggt gggtaatgca ttggttttat ggttaggagc atattatgtg atgagtggtg
     6241 agatgagcat aggagcactg attacttata acgcgttatt agttttcttt acagaaccgc
     6301 ttgagagtgt tattaattta caggtacaaa ttcaaaaagc acaagtagct agtcgacgaa
     6361 tgaatgatat actatcgata gaagttgaag gaaatatcag taacgataca gttgaaattg
     6421 aagaaatggg agaagcggat ttaaagtttg acgatgtata tttctcatat gggcttggtg
     6481 aattgatact aagaggcata acatgtgaaa ttaaagtggg tgataaaata gcattagtgg
     6541 gtgaaagtgg atcaggtaaa tctacgttag ctaaaatgtt agttcggttt tatgccccct
     6601 ctatgggaaa tatttatttc ggaaagcatg atattaatac aattgatgtg catattttaa
     6661 gacataatat agtatatgtt ccacaagatt cattcttttt ggcaggaacg attcgagata
     6721 acttgttatt tggattagac tacgagccga ctcaaaatca aattgaacaa gcttgtgaag
     6781 tagcgtgtat taaatcattt atagaatcat tacctctgca ataccatacg attgttgaag
     6841 aagcaggatc aaatttatca aaggggcaaa aacaacgctt agcgattgtt agagcattac
     6901 ttacgaactc acaagttatt atttttgatg aagcaactag tggtatagat gtcttctccg
     6961 agcaaagaat aatggataat attataaaaa tgacagaacg tacgataata tttattaccc
     7021 atagcttacc tgcggctaaa ttatgtgaga aaataatggt tatggatcaa ggagatttag
     7081 ttgaagaagg aattcatgaa gtgttgagat atgctggagg aacgtatcaa aagttatggg
     7141 aagttatttc ttagttagtg aattgaatat aaaaaccgtt acaattatac ttcataagta
     7201 gttgtaacgg cttttatatt agtttttatt tggattagaa aatttttctt tgattgattt
     7261 agcacgtctg aaagcatact gaataatatg attgtttttt aggacaatat cacgattttt
     7321 ataattgata gacacaacat taccagggtt aattaaagct gatttggaaa tacggaataa
     7381 ttcagggaaa ttttcttcgc actttattaa acttccagaa aattcatatt cacccaatgt
     7441 agtggtcagc gttaatttgt gtgattcttc aatactagtc tgtaggtata aaactttctc
     7501 aaaatcaata ttaactgtct ctccatatct agaaaaagta aataattgtt ttttttcgtc
     7561 aagagaggta gttaatctat tatatgcgat gttcagtgtt tcaaaaatat ttttgcgtaa
     7621 ttcctctaca ttagacggtt tttcaatgaa tgacatagct tctaaattac gcttaaatgt
     7681 taaaggagca gcgtgttcat ccgttgtaat aaatactatt tttgcattta catcattttt
     7741 acgaatgact tctgctatat ccaacccgtc gattgtacta tttaggtgaa catctaacaa
     7801 gaagactcct tttttgggtt gatatgtttc taaataatct aaaacttcgt gtggagaaga
     7861 tgttattaat gacacattaa aacgagattc ataaaaacct gtataaaggt taataatttg
     7921 tttgattgcg tttaattgac caattgagtc ttcgcaaata atgagtggat acattggctg
     7981 aatcctttca attataattt ggtagtaata atttttgcag aaaaaatggt gttcttttcg
     8041 aataacactg tgagattatt atgttttttc tcaattttag ttattgttga taatcccatt
     8101 ccgcgatcat ttcctttcag agaaaatcct tcttgtttaa aattacggag ggtgtaatga
     8161 tcaggtatgt agggattaga aataacaagt tccatcactt tagtgtggct agtaatcaat
     8221 atatcaatat gtttttcttc aattgtcgta acgtattctt gtgcattatc taataggata
     8281 ccgaccacac gaatgaaatc aaatgtatct atatatatta tctagtggct tacgacattc
     8341 aaattgacag gtaattccca attcggtaaa ggtaactaat ttagctagaa taatactctt
     8401 tagagcagga tgtttgatat tatgaatatc tttatattga aatgttgagt gactgaaata
     8461 gtctttggaa taatcgatta atgaatgcat catttctttc agaccagcta agtcattgtt
     8521 ttcaataaaa ctggtggtgg atagcattaa atttttatag tcgtgttgaa attctttagt
     8581 tttataggtt tcttcttcga caatatgatt atatttagtg agtgcttcta aatgttgatt
     8641 ggatagttcg tgttggaatt ttaattgtaa atatttgtgt aaagcatata aaaaacctac
     8701 agtaatgatt acaaatatga tagttatggt aagtgtaaaa taaagaagtt tacgatacaa
     8761 atcaaaccat tgaataaaga taaaatatga tgagaataaa atattgagga ttaatagtga
     8821 gtatgttact attttttttg ccttaacttg gtcaatattg gatgtaaaat atgggtagat
     8881 ttttttataa atagcaattg acacaataaa cgttagtatc tttagagtaa tcatgactaa
     8941 tatgtggtta tgtaaattgt aatattttag ggtattgatg ggtaaagcaa ccaagaattc
     9001 tacaattcct ttagtataaa cgagtagaag aattgaatta agtacttcga aatttattct
     9061 tttcaacgaa aaaaatatgc catacaacac taaaccatat agtaaagcca caatatcagt
     9121 ggcataaaaa agatcatgta taatcataga aaaaatccaa acaacgggaa ctgatatact
     9181 tacaaaaatt ctctttaaga caggtttatt ttgagtgaat aaaaaattga acgtagtgaa
     9241 gaacaaagct acgtcaagca atatttcata aataaataga atgattggat taacaaacat
     9301 aaaaacaccc ttataaatta tctatataag ggtattttat catgttatta atatttatgt
     9361 ctagcatctt ttatgttttg acagtaaaca gttaaatata gcacgatgtg tatttgtttt
     9421 attactataa ccaccgtaaa ttttatgtaa ttaacgttcc ttaagtacct taatgtcctt
     9481 tttctaatta gatttgtcat taaaatctcc tccaaataat aattatatat agattaatat
     9541 tcattgtggt cacggtaatt tagtcatcgt aaacgtagta ataatgcttc taaccgttag
     9601 ggtgtttaac agatattacg tttaggagcc aaaagataac gaataggaaa aaattctctc
     9661 taactaacac aagaaaggta ttataaatta tagacggaag ggagggaaga aaatgcttaa
     9721 aacattaaga aaattattta gttataatca gattaatttg ggtagattcc acttagttta
     9781 taacggaaaa aatgtgaagt agtaatttgt tatttaactt aatgactaaa tttccgataa
     9841 gcatgctgtt gtattatgta tatgaaaagt atggactaca agattgtatg gctattcatt
     9901 ggttacataa tatgttaatt gggaaatttg tataaggagt aagtaaaatg tatagaaaaa
     9961 tattattgtc acttattagt acagtgataa tttttggtat agggatttat ttaaatgtat
    10021 caatgtctct tttgatgttt gctgtaatat catatgtatt accgttaatg ggcaatatag
    10081 taattgatta ttatgtacaa acagagaata tattaatagg aagtggtatc atttctacga
    10141 ttacaacgac gggttatgct atttttgcga tattgatgga aaataatatc aattttgatg
    10201 gatttattag acagcacaca tatcgtagcg ggaatatgac aatgggactt gaaccgggat
    10261 tagcagatat gagtcaatta atttttgttt tcgcattgaa tctatccgta ttatatttca
    10321 tacaatattt aagtagagga gatttttcgc atgttagacg taagtaatgt aagcaagcaa
    10381 tatagtggta atgattttta ttcactaaag gacgtcagtt ttacgataga aaaaggagaa
    10441 attgtcggtt taatcggaaa gaatggagcg gggaagacga cattactgaa gttgttagcg
    10501 aagtctcact tcccaacggc tggagtgatt cggtatcaag ggctggatat attctcaaaa
    10561 gataatgtct tgcgtccatt tgggttgatg attgagacgg tttattacag tcatttaaca
    10621 gtgaaacaaa atatagaatt ttacttagat attcacgatc aatcagaata taagaaaaat
    10681 attcagtccg tactggaaac agttgagtta tggcacaaga aagattttaa gccagatggg
    10741 ttctcttttg ggatgaaaca gcgcttgtca ctggcaatga gtttagtgac gaatcctgaa
    10801 tttatgattt tagatgaacc atttgtagga ttggatccag atggcgtgaa tgatctgatg
    10861 cgtattttga agcagtgggc tgcagatcgt ggcacatcaa ttattgtctc aagtcatcaa
    10921 ttgaatgaat tagaagccat ttgtgatcgt tatttgtaca ttgaaggtgg tgcacttaag
    10981 aaacaatttg gcaaagcagc tgttgaagtc actgtgattg aattggtcga tgcactgcaa
    11041 gacagagacg cgttattcag tgcatatgaa gctattcagg ccatctcaga agacggcaaa
    11101 caaattgaag tcgattcaaa tagtgcagcc ttccaccaaa ttttgggtac tattacagct
    11161 agccataata tcaaaaagat tttcaccaaa gaaaatgcat taaatcaata tttcaaaggg
    11221 agcagagacc atgagtaaac ttaaccaagt tgtatttcac aatacattgc gcaaaaaaga
    11281 agtatgggta tatctagcat ttacccttct gccactcctt gtttttgtga cggaattaac
    11341 ggacacgaaa ttcttacgct taacgggcga tttttcggag atgaactttc ttgattttta
    11401 tggaacgacg ctggggatta ttgatggcat gattctgccg actatcgtca ttgcctatat
    11461 tgcctctacg atgttctacg gcgaaattaa caaggggatt ttatttttat acaaagatat
    11521 taatcgaaaa aaagttatga attcaaaggt gttctcgctg atgaaaacgt atgggttgta
    11581 tctattattg gtcatgctct catcgttagt gatttactac ttcttcgtta tccagatgaa
    11641 aggtggcacc ttttcgcttc taggtagtgt agcacctgtg acgtattata gcgtgttagg
    11701 cgtgttaggg ctgttcggga tagacattgt ggcgatattg gtagcagcta atttatctgt
    11761 acgattctca acaggtgtca cagtgttggg gacgattttc ttcctgttcc tcatgcaagc
    11821 tattagccga gtcgatggct tgaattactt atcgccaatg agttatcgcg agctatttaa
    11881 tgttggacag atgacgttta gtatgagttt tatactgcta gttttcttgt gtgtggtgta
    11941 tgtcgtgagt gcgtacacct tgttgtaccg gcaatttagc aaggtggagt attaacgtga
    12001 atagaatcag gagaatgact aatgacaaca tttaaccaag tgctgttaaa aaataactta
    12061 atgaagtggg aggtctggct ttatttagca ttcgcgctgt atccactact tgtcttcatc
    12121 gggcaattgt ttcaagtgaa ttttatgcaa ttag
//
