LOCUS       NZ_CP040417            12154 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3077 chromosome.
ACCESSION   NZ_CP040417
VERSION     NZ_CP040417.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 351x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655765.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:25:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,806
            Genes (coding)                    :: 1,779
            CDSs (with protein)               :: 1,779
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 12 of 27
            Pseudo Genes (incomplete)         :: 15 of 27
            Pseudo Genes (internal stop)      :: 6 of 27
            Pseudo Genes (multiple problems)  :: 6 of 27
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:08:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1410769
            Orig. end                         :: 1422923
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7154)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            120..260
                     /locus_tag="FE328_RS06685"
     CDS             120..260
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS06685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_181452867.1"
                     /transl_table=11
                     /translation="MKFYEDNKKLIHIIIGVLVFLAFVGAFGTIYDYGKDFGRSLYLFF
                     T"
     gene            366..863
                     /locus_tag="FE328_RS09420"
     CDS             366..863
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /locus_tag="FE328_RS09420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="WP_244325327.1"
                     /transl_table=11
                     /translation="MVDCDLKQCFDTLNHDKMMYHVEQFVQDKAILKFIRKSLRCGTIE
                     LSGEFTDSETDAPQGGVISPLLCNIYLNELDKELERRGHKFVRYADDFIIFKSSKRVCE
                     RILKSITQFIEKDLKLQVNQEKSKTGSPTQLKFLSCLIHNNFGNCRFRPTDETKAKFKK
                     KT"
     gene            904..1239
                     /locus_tag="FE328_RS09425"
     CDS             904..1239
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /locus_tag="FE328_RS09425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="WP_244325364.1"
                     /transl_table=11
                     /translation="MVKEINQVTQGWINYFGLDFVKRFVRRTEEWLRRRLRQLILQRWK
                     KCSTKIKMLQKYGLTEDEAKRIAFSRKAYWHLSQTYEVNKAITTKRLHKWGLKSLTTIA
                     ESDYARY"
     gene            complement(1365..1607)
                     /locus_tag="FE328_RS06695"
                     /old_locus_tag="FE328_06625"
     CDS             complement(1365..1607)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS06695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_06625"
                     /product="hypothetical protein"
                     /protein_id="WP_077863095.1"
                     /transl_table=11
                     /translation="MSDKEKVLNGYMFCAILVLNILWFLLNYSLSAFDFWGTVFSLNSY
                     ISIVVIIGYASMLISAKKKGEIDSYWIKDNQEKDK"
     gene            complement(1626..2207)
                     /locus_tag="FE328_RS06700"
                     /old_locus_tag="FE328_06630"
     CDS             complement(1626..2207)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_06630"
                     /product="hypothetical protein"
                     /protein_id="WP_077863096.1"
                     /transl_table=11
                     /translation="MMKKKLGATVATVLLGVSAIPFNVSAHEEVTTEEVQIQHGENNQE
                     HHEEQIGQMTLVNEHTGVLNFNEGITHAFNGDGTVTLTDSQGNSEVLPQETVDKNGENV
                     RLVYEEAQNGQLAVTAVLPQENGRQKRSAGKCLAGIGAGAFTTGTSLGIKGAGAGTVAV
                     PGVGTVGLGVVGAVGGAIGGGLGGAAASCF"
     gene            complement(2410..2544)
                     /locus_tag="FE328_RS09490"
     CDS             complement(2410..2544)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS09490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_258392289.1"
                     /transl_table=11
                     /translation="MKKLTNRELRLTVGGSYSSACKRVWNAKPGDITIALYCPLPKVK"
     misc_feature    complement(2561..2578)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.05"
                     /tool="antismash"
     gene            3076..4206
                     /locus_tag="FE328_RS06705"
                     /old_locus_tag="FE328_06635"
     CDS             3076..4206
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635172.1"
                     /locus_tag="FE328_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06635"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_244325328.1"
                     /transl_table=11
                     /translation="MVLYGIVIYRYFLNDRANRKRELIHILLFVLIKGSIEFLTALPIN
                     LFALGHLHNHVGVFLLASIVDVGLSVIAYKQVIKYFKNITLSSYETDILRHVLLGYNLA
                     LIFYFGYMKHQALYFDLLVFTSVALVGNGIVVGLVVFYVLRYSETKTREEAISKRLDYM
                     VKYTNIIEENQLELKRFKHDYQNMLLSLSILAEAGNLDKIKQKTASLRQYSAQQLLKEY
                     TQYSDLARVHHTLLKSILIAKLTELHHMNIVFRFVCPEPVESLNIADFDLIRMVGILID
                     NAREHVKDMEDGMIAVSVHKTTRGTHIKIENTYANQVTSIATLMTPGYSSKDDHSGLGL
                     SNVEKIKNKYDHVFLNYRIDDKFEVTLIIMNEEGEY"
     gene            4207..4944
                     /locus_tag="FE328_RS06710"
                     /old_locus_tag="FE328_06640"
     CDS             4207..4944
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="FE328_RS06710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06640"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_208957791.1"
                     /transl_table=11
                     /translation="MHSIIICEDNQEQLAYLSMLVQNYIQFHENQFRLVYEGTDPQTTL
                     NYIQQEAIKKGLYLIDINLGADMTGVDLAEAIRDTDIQSKIIFITSEEDQATHILNRHI
                     EPLAYIMKNEALETMQSNLHRALDDAYSRFSEITVRTKDVFSFNFDTLTYQFDLDEVIS
                     IEVSGDHRLTLKTITGQYDFFNTLAQIEQDYPTLLRIGRSDMINPVNIKHIDYKRRNVT
                     MINDEQFTIAASRILLLRQLYKS"
     gene            complement(5001..7154)
                     /locus_tag="FE328_RS06715"
                     /old_locus_tag="FE328_06645"
     CDS             complement(5001..7154)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292468.1"
                     /locus_tag="FE328_RS06715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06645"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_208957792.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.3e-43, bitscore:
                     144.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.7e-238, bitscore:
                     790.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDTFKFVKQQDIMDCGVACIQMILKHYDSDMPAHKLRHMTGTDI
                     DGTSALGLKSALEELQFECLAVQADNSVWTDPDMNYPAIAHVLLEDNRQHYIIIYGFKN
                     NKLLIADPAEGKYKMSPEEFTTIWTNILLIPKPNKQYQSVVEKIGGLTSFLPALFKSKK
                     IMFFTIIASFLATSLSIVSSYYFQGIIDRVIPQEDLSLLNIMSIGLLGVYLLRVLFDYI
                     RSQLLIILGQSMSSHIMLDYFKHVLYLPMQFFNTRKNGDIISRFLDANKIIRALASSAL
                     ALFLDITMVAIVGTFLFIQNRILFVITLLSLPIYFVTVLFFVKRYNKANEEEMSSAAIL
                     NSSIIESLDGMETIKSYNSEQNVYETVQNQFLNLMKKSYKTVTLDNMQRSIKQSVQLLT
                     SAGVLWAGSYYILHNSMSIGQLITYNALLVFFTNPLENIINLQAELQTAEIASKRMNEV
                     LAIESEYNLTDKQAAITFDGGIRINHLTFSYNLKESTLKNITCHIPFNQTVALVGMSGS
                     GKSTLAKLLLRLHEVAEGTIQYDNILINKIPHNYLRDHVTYLPQESFFFKGTIIENLLF
                     GLSHQPSELEIITACERAKVRHVIDSLPLGFNTPLEEGAANLSGGQKQRLAIARALLRD
                     TNIYIFDEATSSVDTVTEQKIIQSISELKNKLIIHITHHLPIAKQCEQILVMHDGQLVE
                     EGTHEQLLANSGTYHTLWQSTFP"
     gene            complement(7170..7883)
                     /locus_tag="FE328_RS06720"
                     /old_locus_tag="FE328_06650"
     CDS             complement(7170..7883)
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /locus_tag="FE328_RS06720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06650"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_208957793.1"
                     /sec_met_domain="Abi (E-value: 2.3e-13, bitscore: 47.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSFIKKIGSPVKIIFLGIIFSVIATLIISVGQIPRDIFPAFVEN
                     NMPYISRYIYTIVAFILLIWWLKWWNKSVTPIYMNNTLFQKKNWVYGIGGYVLIFILVL
                     LFILAKGYIFGSNSVEISKTLLDEFSFEQNMHLGSLSILYIIFLGPILEEFIYRLGILG
                     QLVEMNTPTWLAVILSVLIFSFSHHNGIISQHLISGLGYSLLMIKTKSIYPGIIAHILF
                     NTTTLIYRWYMLYYL"
     gene            8329..9801
                     /gene="gltX"
                     /locus_tag="FE328_RS06725"
                     /old_locus_tag="FE328_06655"
     CDS             8329..9801
                     /EC_number="6.1.1.17"
                     /GO_component="GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gltX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635166.1"
                     /locus_tag="FE328_RS06725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06655"
                     /product="glutamate--tRNA ligase"
                     /protein_id="WP_112779159.1"
                     /transl_table=11
                     /translation="MSEAIRVRYAPSPTGNLHIGNARTALFNYLFARHNDGTFIIRIED
                     TDKKRHVDEGEESQLKYLQWLGIDWDEGPVQGGDFGPYRQSERNELYQKYVDELLARDL
                     AYKCYVSSEELEEMREEQRANGVMPHYDGRHAHLSEEEQAQFEAEGRQPVIRVRVPEQT
                     TYTFDDMVKGDISFESKDIGGDWVIQKVDGTPTYNFACAVDDHHMKISHVLRGDDHISN
                     TPKQLMIYDAFDWEPPRFGHMTLIINAETGKKLSKRDESILQFIEQYNNLGYLPEALFN
                     FITLLGWSPKGEEEIFTKQEFIDMFDYKRLNTSPAAFDQKKLEWITNQYIKELPDEDVV
                     ALIQPHLVEAGVFPADPSEADREWVTKLANLYKEQMSYGQEIVSLADLFFGEGIAFDEG
                     AREVLSGETVPTVLEAFKKHLADVDVFEKDNIFPAIKAVQKETGVKGKNLYMPIRVATT
                     GQQHGPELPLAIELLGKHKAISHIEQALEVIG"
     misc_feature    complement(9906..9920)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.16"
                     /tool="antismash"
     gene            10176..11750
                     /locus_tag="FE328_RS06730"
                     /old_locus_tag="FE328_06660"
     CDS             10176..11750
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="FE328_RS06730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_06660"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_208953404.1"
                     /transl_table=11
                     /translation="MDKKAKVLIGVLLVISGLETVFNAATYAMVFDIIEQQKLNLVVVY
                     TVVVILGYILFSGIRHIKDRTINHYVKEEKASIQKKILDNEEQKFKLFEHTGSSDKMSF
                     FQNDLKLFEDNYLRKKFEIISQVIVLLGVLAFSLYSNFLLTVIFSLFAAVPHFVSGFMN
                     KKIQQSTEHWTQATAKANHSLIDLFKNFWTLMVYHATNKEIKSVSEDILKKEAANATMQ
                     NTISLAQMSTMMVGYTMMMIPIGIGMYLTISGDLPIATFVAVQYSSSMIINSLLTTVRL
                     KNELQSAKPIKDKIDKELSFVPRSTRSKKLNGHIQSVVFKDVSFSFGETKVLDNFNLTL
                     NGVDKVLLQGESGSGKTTIFKLLTKQLLPDSGTIYINGIDTRELDIEDILQCFGYISQQ
                     ALVFDDTIEKNITLGHDFSTEEIMKACEMAQIGELIKKTGLDYVIGEDGQNLSGGQLKR
                     LEIARAILFKRKGLLVDEALSSLDKETGRAIVKTLMDLDKLMIDIEHHIAAELTEGYTD
                     VITLSRS"
ORIGIN
        1 catcagaaaa aaattaacat aatgtttagt aaaataataa atgtatatgt gttatctgtt
       61 agatgataac acaatacatt tattatttta tactctgagg aaagaaagga agtatattga
      121 tgaaatttta tgaagataat aaaaaattaa tacacattat cataggtgta ttggtatttc
      181 tagcatttgt tggagcattt ggtactattt atgactatgg gaaagatttt ggcagaagtt
      241 tatatctttt tttcacctaa tgattgaata ttgtaatcaa aaaagaaagg cttcaccact
      301 agtgattaaa tctaattaat tcctagcggt gaagtcttga atattacgaa tctggcttaa
      361 aatatgtggt agattgtgat ttgaagcaat gcttcgacac acttaatcat gataagatga
      421 tgtatcacgt ggaacaattt gttcaagata aagctatttt aaaattcata cgtaaatcat
      481 tgagatgtgg caccattgaa ctatctggcg aatttacgga tagtgaaact gatgcaccgc
      541 aaggtggtgt gatatcacca ttactgtgta atatctatct gaatgagcta gataaagagc
      601 ttgaacgacg cggacataaa tttgtgcgat atgcggatga ttttataatc tttaaatcat
      661 caaaacgagt atgcgaacga atcctgaaga gtataactca attcattgaa aaagacttga
      721 aacttcaagt caatcaagaa aagagcaaga caggaagtcc aactcagtta aagttcttga
      781 gctgtctaat ccacaacaac ttcggcaatt gtcgattccg accgacggac gaaacgaaag
      841 caaaattcaa gaaaaaaact taagagatta accagacgca atcgaccagg aatatttacg
      901 gagattgtaa aagagatcaa ccaagtgact caaggttgga ttaattactt tgggttagat
      961 tttgttaaaa gattcgtgag aaggactgaa gagtggttgc gaaggcgact tagacagctt
     1021 atattgcaac gttggaagaa atgctcaacc aagattaaga tgcttcagaa atatggttta
     1081 acagaagatg aggcaaaacg aattgccttc tctcggaaag cctattggca tctatctcaa
     1141 acatatgaag tcaataaggc aattacaaca aagagactcc acaaatgggg attaaaatca
     1201 ttaaccacca tagcggagtc tgattatgca aggtattgaa ccgccgtata cggatccgta
     1261 cgtacggtgg tttgagaggg caactagtca attaatggct agtctcctac tcgatttatc
     1321 ttcataaaat tttaccaata tacttccttt cttcccatta tattttattt gtctttctcc
     1381 tgattgtctt ttatccagta actatcaatc tcgccttttt tcttagcaga aataagcata
     1441 gatgcgtagc caattatcac tacgatactt atatacgaat ttaatgagaa aactgttccc
     1501 caaaagtcaa atgctgaaag tgaatagttt aataaaaacc aaagtatgtt aagtactaat
     1561 attgcacaga acatatagcc attcaacact ttttctttat cactcatatt ttctcatcct
     1621 ttcaattaaa aacaacttgc agctgctcca ccaagtccgc caccgatagc tccgccaaca
     1681 gcacctacaa cacctaaacc tacagtacca acaccaggaa ctgcgacagt ccctgcacca
     1741 gctcctttaa tccctaaaga agttccagtt gtaaaagcac cagcacctat tccagcaaga
     1801 cacttcccag cgctgcgctt ctgccgtcca ttctcttgtg gcaacacagc tgttacggct
     1861 agttgaccgt tttgagcttc ctcgtagact agtcgcacat tctcgccgtt cttgtctact
     1921 gtctcttgtg gcaacacctc gctatttcct tggctgtctg ttaatgtaac ggttccatct
     1981 ccattgaaag catgtgtgat gccttcattg aagtttaaga cacccgtatg ctcatttaca
     2041 agtgtcattt gtcctatctg ttcttcatga tgctcttggt tattttctcc atgctgtatc
     2101 tgtacttctt ccgtggttac ttcctcgtga gcagatacat tgaatggaat agctgacacc
     2161 cctaacaaaa ctgtagcaac tgttgcacct aattttttct tcatcattgt aatcgactcc
     2221 ttctaaagtt tttaatactt catttactta tgaagtgtta cctaaattct aacaactagc
     2281 ttttagaaat ataagtatca cccgaatagt tccctgaatg attaaatgat tcaacgaaat
     2341 cctttgataa atgtttcaca tctattatag gttgttccat tatttgcatg ctcctttcta
     2401 ataagtatat tacttaactt taggcaatgg acagtacagt gcgattgtaa tatcaccagg
     2461 ttttgcgttc cacacgcgtt tacatgcact tgaataagat cctccaaccg ttaatcttaa
     2521 ttcacgattt gtaagttttt tcattacgat tcccccttcg ttaatattag tataacaata
     2581 aaaaattata atccttccat ttttcgtgaa ttggtggatt tgagtcccta aacagtaaaa
     2641 ttaaattttt gtcccttgtt cagggaatga tattagccaa ttcgcggaat tttggtgact
     2701 agatagattg actatgatat gattttattg aataatagag aggaggaact ctgatgaaaa
     2761 aattaacaac ccaacagttg aaaaatattt taggtggaca gcttcataag aatagtcact
     2821 cgtgggctaa actaaaatgc ttgttcaacc taatgagaaa ttgttaaaaa tagcgaaagg
     2881 gtgagggtga ttttaattaa tccagtggta gctgttatat tagaaatagg cttgcattat
     2941 atttttatga caatagcttt catttatcta tttaatattg aaaaaaaatc tatcacaaga
     3001 ttatttcttt tgggcactct attttggctt ttatcactct ttttaagtga ttatctacgt
     3061 ataggtagcc tttcaatggt tctatatgga atagtcatct accgttattt cttaaatgat
     3121 cgtgcgaatc gtaagagaga gcttattcat atactattgt ttgtacttat aaaaggaagt
     3181 attgaatttt taactgctct accaattaat ttatttgcgt taggccatct gcataatcat
     3241 gtcggcgtat ttctattagc gtcgatagtt gatgttggtc tctcagtaat tgcatacaag
     3301 caagtaatta aatattttaa aaatattacc ttatcatcct atgaaacaga catccttaga
     3361 cacgtactat taggatacaa tttagcacta attttttact ttgggtatat gaagcatcaa
     3421 gcgttatatt ttgatctgtt agtattcaca agtgttgctc tagtagggaa tgggattgtc
     3481 gtaggcttgg tcgtgttcta tgtattgcgt tattccgaaa cgaagacacg agaagaagca
     3541 attagcaagc ggttagatta tatggtgaag tacacgaata ttattgaaga gaatcaactt
     3601 gagcttaagc ggttcaagca tgattatcag aatatgttac tgagcttgtc tatcttagca
     3661 gaagcaggga acttagataa aatcaaacaa aaaacagctt ccttgagaca atattctgcc
     3721 caacaattat tgaaggagta tacgcaatat agtgatctag cgcgcgtaca ccacaccctc
     3781 ttgaagagta tcttgattgc gaagttaacg gaacttcatc atatgaatat tgtgttccga
     3841 tttgtttgcc ctgaaccggt cgaatcgttg aatatcgctg acttcgattt aatccggatg
     3901 gtaggcatac tgatcgataa tgcgcgcgag catgtgaagg atatggagga cggtatgatc
     3961 gctgtatctg tccacaaaac gactcgtgga acccatataa aaatcgaaaa tacatatgcc
     4021 aatcaagtga cctcaattgc gacactcatg acgcccggat attcatctaa agatgaccac
     4081 tcaggactag gactatcgaa tgttgaaaaa ataaaaaaca aatatgatca tgtattcctt
     4141 aattatcgca ttgatgacaa attcgaagtc actctgatta taatgaatga ggaaggagag
     4201 tactagatgc attctattat tatttgtgaa gataaccaag agcaactagc ctatctatcg
     4261 atgctcgttc agaattacat ccagtttcat gagaatcaat tcaggctagt ctatgaaggc
     4321 accgatccac aaactacctt aaactatatc caacaagaag caatcaaaaa aggactctac
     4381 ttgattgata ttaacttagg ggctgacatg actggagtag atctggcaga agctattcgt
     4441 gatactgata ttcagtccaa aattatcttc attactagtg aggaagacca agcaactcat
     4501 atactgaacc gtcatattga accattagcc tatattatga agaatgaagc attagagact
     4561 atgcaatcta atctacatcg tgccttagat gatgcataca gtcgcttcag tgaaataacg
     4621 gttcgaacca aagatgtatt ttcgtttaat tttgatacat taacctacca attcgatttg
     4681 gatgaagtga tcagtattga agtttctgga gatcaccgac tgacgctaaa aacgatcacg
     4741 ggacagtatg atttttttaa cacattggcg cagatcgaac aagattatcc cacactgctt
     4801 cgtattggac gatctgatat gatcaacccg gtgaacatca agcacatcga ttataagcga
     4861 cgaaatgtga cgatgataaa tgatgaacag ttcaccattg ctgccagccg gatactacta
     4921 ttaagacaac tgtataaatc ataataaaaa agatatgatg catggataac aaacagatag
     4981 catcatatct ttttttgagt ttatgggaaa gttgattgcc ataatgtgtg gtacgttcct
     5041 gaattggcta gcaactgctc atgagtccct tcttctacaa gttgaccatc atgcattaca
     5101 agtatttgct cacattgttt agcaataggc aggtgatgag ttatgtgaat aattaattta
     5161 ttttttagtt cagaaatcga ttgaataatt ttttgttctg tgactgtatc tacactactt
     5221 gtcgcttcat caaaaatata aatattcgta tctctaagta acgctcgagc aatagctaga
     5281 cgttgttttt gaccaccaga tagattagct gctccttctt ctagaggtgt gttaaatcct
     5341 aatggcaagc tgtcaatcac atgtcgaacc tttgctcgtt cacaagcagt tataatttcc
     5401 aattcactag gttggtgaga taacccaaat aataaatttt caataattgt tcctttgaag
     5461 aaaaaagatt cttgtggaag gtatgtcaca tggtctcgta aatagttgtg tggaatttta
     5521 ttaatcagta tattatcata ctgaatagtc ccttcagcta cctcatgaag tcgtagcaac
     5581 aatttggcta gagtagattt tccagagcca ctcattccga ctagagcaac cgtctgattg
     5641 aatggaatat gacatgtaat attttttaga gtagactctt ttaagttgta agaaaatgtc
     5701 aaatgattta ttcgtatccc tccatcaaaa gtgatagcag cctgcttatc agtgagattg
     5761 tactctgatt caatggcaag aacttcattc atacgtttac ttgctatttc agcagtctgt
     5821 agttctgctt gtaagttaat aatattttct agtgggttag tgaaaaatac taataatgca
     5881 ttataggtaa tcaattgacc aatactcatt gaattatgaa gaatataata tgaacctgcc
     5941 cataaaaccc ctgcactagt gagtaactgg acagattgtt taattgaacg ttgcatatta
     6001 tctaatgtaa cggttttata ggattttttc attagattta agaactgatt ttgaacggtt
     6061 tcatatacat tttgttcgct attatatgac ttaattgtct ccatgccatc caagctttca
     6121 ataatacttg aatttagtat tgctgcggaa ctcatttctt cttcgttcgc cttattataa
     6181 cgcttcacaa aaaataatac agtgacaaag tatataggaa gtgatagaag tgtaatgaca
     6241 aataagattc tattctgaat aaataaaaat gttcctacaa tagcaaccat cgttatatcc
     6301 aggaatagag caagagctga actagcaagt gctcgaataa tcttattcgc atctaaaaat
     6361 cgtgagataa tgtctccgtt ttttcgtgtg ttaaaaaact gcataggaag atataggaca
     6421 tgcttaaaat agtctagcat aatatgacta ctcatgctct gacctaaaat aattaacaac
     6481 tgacttctaa tatagtcaaa caatactcga agtaaataca cacctaataa tccgatagac
     6541 ataatgttga gtagagatag atcctcttga gggataactc tatcaataat tccttgaaaa
     6601 taatatgaac taacaatact cagtgatgta gctaaaaatg aagctattat agtaaaaaac
     6661 attatctttt tagacttaaa taaagccggt aaaaacgagg ttaatccacc aatcttctcg
     6721 acaacagact gatactgttt gttaggttta ggaataagga gaatatttgt ccaaatcgta
     6781 gtaaattctt cgggactcat cttatatttt ccttcagctg ggtcagctat taaaagttta
     6841 ttattcttga atccataaat tataatgtaa tgttggcgat tgtcttctag taagacatgg
     6901 gcaatagcag gatagttcat gtcaggatct gtccaaacac tgttatctgc ttgaacagct
     6961 agacattcaa attgtagttc ttctaatgcg gacttcaatc ctagggcaga agttccatca
     7021 atatcagtac ctgtcatgtg ccggagttta tgggcaggca tatcagagtc atagtgtttt
     7081 aaaatcatct gaatacaagc cacaccacaa tccataatat cttgctgttt aacaaattta
     7141 aaagtatctc gcataataat attactcctt tataaataat atagcatata ccaacgataa
     7201 ataagtgtgg tcgtattaaa taagatatga gcgattatgc ctgggtaaat tgactttgtt
     7261 tttatcataa gtagggagta gccaagtcct gagataaggt gttgtgagat aatgccatta
     7321 tgatggctga acgagaatat caatacagat aaaataacgg caagccatgt gggagtattc
     7381 atttcaacta gctgcccaag aatacccaat cgataaatga attcctctaa aataggccca
     7441 aggaagataa tgtataggat ggataagctg cccaggtgca tattctgttc aaatgaaaat
     7501 tcatcaagca gtgttttaga tatttcgaca ctgttacttc caaagatata accttttgct
     7561 agtatgaata aaagaacaag tatgaaaatt aggacatagc cacctatacc ataaacccaa
     7621 ttcttttttt gaaagagtgt attattcata tagatcggag tgaccgactt attccaccac
     7681 tttagccacc aaattaaaag tataaatgca actattgtat agatataacg acttatgtat
     7741 ggcatattat tttcaacaaa agctgggaaa atatctcggg ggatttgtcc aaccgaaata
     7801 atgagagtag caatcacaga aaatataata cctaaaaata taattttaac tggactacct
     7861 atttttttga taaaactagt catatgtgta tctccttaat aagattgtta tgaatatatt
     7921 cactatacca gatatttaac aaatcgctgg aatatttcgt gaatcggtgg atttgagtcc
     7981 ttgaactggg aaatacagtg aattctttct attcaggagt tcaatgacgt ccaaaaacta
     8041 gagcatgttc ggtaaataca tattaaaaat ggatccatta attgtgggtt tagtattaaa
     8101 tatgttttct aagaagagtt tttaatatta gttcagaagc aaaaaaatta aatttttaaa
     8161 cagagtctac ttagtttatg agtaggcttt ttttgttggg cggtggtctt ctagttacat
     8221 cgccgtgggg gttatttgaa ttgttcgctt atggacggtg tttgtggtat gatgtggggt
     8281 ggatataaga attgaaattg taaggttata ataaggagtt tgatattgat gagtgaagct
     8341 attcgtgtac gttatgcgcc gagtccaacg gggaatttac acattgggaa tgcgcggacg
     8401 gcgttgttta attatttgtt cgcgcgacat aatgatggga cgttcattat tcggattgaa
     8461 gatacggata agaagcgtca tgtggatgaa ggggaagaga gccagttgaa gtatcttcag
     8521 tggctgggaa ttgattggga tgaagggcct gtgcaaggtg gcgacttcgg accctaccgt
     8581 cagtcagagc gtaatgaatt gtatcagaag tatgtggatg agttgcttgc gcgtgattta
     8641 gcatacaagt gttatgtgag ttccgaagag ttggaagaga tgcgcgagga gcaacgggcg
     8701 aatggtgtta tgccacatta tgatgggcgt catgcgcact taagtgaaga agagcaagcg
     8761 cagttcgaag ctgaaggacg tcagccagtt attcgagtac gtgttcctga gcagacaact
     8821 tatacgttcg atgatatggt taaaggagat atctccttcg aatcaaaaga tattggtggg
     8881 gattgggtca ttcagaaagt agatggcacg ccgacatata actttgcgtg tgcggtagat
     8941 gatcatcata tgaagatttc acacgtttta cgtggggatg atcatatctc gaatacgccg
     9001 aagcagttaa tgatttatga cgcatttgac tgggagccac cacgatttgg tcatatgaca
     9061 ttaattatta atgcagagac gggtaagaag ttatcgaagc gtgatgagtc gattttacag
     9121 tttattgagc agtacaataa tctaggttac ttacctgaag cgttgttcaa ctttattacc
     9181 ttactcggtt ggtctcctaa aggtgaggaa gagatcttca cgaagcagga atttatcgat
     9241 atgttcgatt ataagcggtt gaatacatca ccggctgcct ttgatcagaa gaagttagag
     9301 tggattacga accaatatat taaggaactg ccagatgaag atgtggtagc gttgatccaa
     9361 cctcatttag tggaagcggg tgtgttccca gctgatccat ctgaagcaga tcgggagtgg
     9421 gtaacgaagc tggcgaatct gtataaagaa cagatgagct acgggcaaga gattgttagt
     9481 ttagctgatt tattcttcgg ggaaggaata gcgttcgacg aaggggcaag agaggtattg
     9541 tctggagaga cggtcccaac tgtcctggaa gccttcaaga agcacttagc tgatgtggat
     9601 gtattcgaga aggataatat cttcccagcg attaaagctg tgcaaaaaga aaccggtgtg
     9661 aaaggaaaaa acctgtacat gccgattcga gtggctacaa ccggtcagca gcacggacca
     9721 gaattaccac tagcgattga attattaggc aaacataaag cgattagtca tattgaacaa
     9781 gcccttgaag tgattggtta gtcggtcaga atgaagtaaa taggtgttaa aaactgggag
     9841 ctgtgtaccc atcaactccc agctacatag gacaagtgat cggaattggt caaaacgaac
     9901 gaattctctc ataatgagaa ttcgttcttt aaattgttaa cgaataatag gaggtaaaat
     9961 taataaacaa caaaatgtaa ccgatttaat taaaatacca gacttttttg actcaaaaac
    10021 gactaaattt aataaaaata ttagaaaaaa aggaaaaaac agtgtacaat taaggcatca
    10081 acatttattg tttgttggtg cctcttttta tagggtgata acagggaagg agtctgcaac
    10141 ggaagtagaa atttataaaa taaggagaag agaacatgga taaaaaagca aaagttttga
    10201 taggggtcct tcttgtcatt agtgggctag aaacagtttt taatgcggca acctatgcca
    10261 tggtatttga tattattgaa cagcagaaac tgaatttggt tgtcgtgtat acagtggttg
    10321 ttattctggg gtatatttta ttttctggaa tcagacatat aaaagatcgt acgattaatc
    10381 actatgttaa agaggaaaaa gcatctattc aaaagaaaat attagataat gaggaacaaa
    10441 aatttaaatt atttgaacat acaggttcat ctgataaaat gtcttttttt caaaatgatt
    10501 taaagttatt tgaggataac tacttgcgaa agaaatttga aattatatca caagtaattg
    10561 tcttgttagg ggtactggca ttttcacttt acagtaattt tttattaacc gtgatattta
    10621 gtctttttgc agcggtacca cattttgttt caggattcat gaataaaaaa attcaacagt
    10681 ctacggagca ttggactcaa gcaactgcta aggctaatca ctcgttaatt gatttgttta
    10741 agaatttttg gacgttaatg gtctaccatg caaccaataa agaaattaaa tctgtgtcag
    10801 aggatatttt aaaaaaagaa gctgctaatg caacgatgca aaataccatc agtttagcac
    10861 aaatgtccac tatgatggtg gggtatacca tgatgatgat tccgattggg attgggatgt
    10921 acttaacgat ctcaggagat ttacctatag ctacatttgt ggctgtgcaa tattcgagta
    10981 gtatgattat caatagcttg ttaacaaccg ttagattaaa aaatgaatta caatccgcaa
    11041 aaccaataaa agataaaatt gataaagagt tatcctttgt gccgcgttcg actagatcga
    11101 aaaaattaaa tggccatatt caaagtgttg tgtttaaaga tgtttcattt agctttgggg
    11161 agacgaaggt gctcgacaac ttcaatctga cattaaatgg cgtagataag gtgttgttgc
    11221 aaggagaatc gggatcggga aagacgacga tttttaaatt actaacaaaa caattactcc
    11281 cagattcagg gaccatttat attaatggga ttgatacacg ggagttagat attgaggata
    11341 ttctgcaatg ttttgggtac atttctcagc aagcactggt gtttgatgat acgattgaga
    11401 agaacataac actaggacat gatttttcaa ccgaagaaat tatgaaagct tgtgaaatgg
    11461 ctcagattgg tgaattgatt aaaaaaacag gacttgatta tgtgatcggt gaagatggtc
    11521 agaatttatc tggcggtcaa ttgaaacgat tagaaattgc tcgtgctatt ctattcaagc
    11581 gcaagggatt attagttgat gaagccttgt catcgttaga taaagagacc ggcagagcaa
    11641 tcgtgaagac gttaatggac ttagataaat taatgattga tattgaacac cacatagcag
    11701 ctgagttgac agagggctat actgatgtga taaccttgtc tcgaagctaa tcaccttgat
    11761 gcatccacgt ggtaaaaaac ggggacctat gatttgaaaa tctgaattgc aacatgattt
    11821 gaaaaagaag aatcattcga ttaccgctat aagttatggt aaaatgtaag agcatactta
    11881 taatgttgag ttatgaagat gttggaagaa cctttcttgg ataagaaggg tctttcgatt
    11941 atataaatat aaaaaggatg agtcaatgga gtatcatgtg ctagaggaga agttaccggt
    12001 gaagccagca ggggagatgg tgctggggct ggagacgatg cgggaattga tggggttgtt
    12061 ggacaatccg caagataagg tgccggtgat tcatattgca gggacgaacg gaaaagggtc
    12121 cgttgctacg ttcttacaag ccgtcttgag gcaa
//
