LOCUS       NZ_CP040417            12157 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3077 chromosome.
ACCESSION   NZ_CP040417
VERSION     NZ_CP040417.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 351x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655765.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:25:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,806
            Genes (coding)                    :: 1,779
            CDSs (with protein)               :: 1,779
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 12 of 27
            Pseudo Genes (incomplete)         :: 15 of 27
            Pseudo Genes (internal stop)      :: 6 of 27
            Pseudo Genes (multiple problems)  :: 6 of 27
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:08:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 377286
            Orig. end                         :: 389443
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..12157
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12157
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12157
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7157](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7157)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(837..852)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     gene            complement(1926..2066)
                     /locus_tag="FE328_RS01665"
                     /old_locus_tag="FE328_01655"
     CDS             complement(1926..2066)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS01665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_01655"
                     /product="bacteriocin"
                     /protein_id="WP_112787934.1"
                     /transl_table=11
                     /translation="MTKYKKLKNTELATIIGGGPLLIIGGVAAAAFGLGAIDGYMEGAN
                     A"
     gene            complement(2084..2218)
                     /locus_tag="FE328_RS09455"
     CDS             complement(2084..2218)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS09455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_258392541.1"
                     /transl_table=11
                     /translation="MRRILTADLMKINGGGVLEGLGDAWTAIYNTGKDFGRSLYDFIN"
     gene            complement(2269..2412)
                     /locus_tag="FE328_RS01670"
     CDS             complement(2269..2412)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS01670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_172763404.1"
                     /transl_table=11
                     /translation="MKQVGKKKLFYIRGGNLSSILCNINTAIKFNGQPLLKICPHSIKT
                     LR"
     gene            3361..4203
                     /locus_tag="FE328_RS01675"
                     /old_locus_tag="FE328_01660"
     CDS             3361..4203
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="FE328_RS01675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01660"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_146737420.1"
                     /transl_table=11
                     /translation="MLIVVMNLITILYFMYIKYTGQYHSLLILTTFFLAVFFLFMGGLC
                     FYLIQFIKSKQEIRFINKQLQSTKEYNKIIENNQLKLRQFKHDYKNILTSLNGLVASEQ
                     YDQLEIKLNQLTQYSKQELKNEKSMYDELINVEHELLKSILTVKLIEMDNEQLNFQFSC
                     PKKITTIGMHSFDLIRVVGILIDNAREYAMDYKGSEIEVIVNRGDDYIDIIIANTFFEN
                     HASIDRFKKKGYTTKLGHKGLGLTKVEEINHQYDNVLVNYRIDDYFSVEITILDREGD"
     gene            4207..4941
                     /locus_tag="FE328_RS01680"
                     /old_locus_tag="FE328_01665"
     CDS             4207..4941
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="FE328_RS01680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01665"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_172763402.1"
                     /transl_table=11
                     /translation="MFPIFICEDSKEQLDRLITIVKYYIMDRDNYYLEKVSTNPEKLLS
                     YVKDKSIKKGIYLLDVNLNHHLTGIDLAEKIRKQDPHAKIIFITGHDESMPEMLKRNIE
                     VLGFVQKNDELDILRKNVLQAISAANERLISTLTEDKDIFTFSFGAKTFRFDFKDVLSV
                     ETTGKSHQLIIYLTNGQYEIRGTLSELEKKYAQFFRLNRSVLINHHNVVRINYSERIII
                     MRNGTTIFFTKHQTKKLKKYFG"
     gene            complement(5001..7157)
                     /locus_tag="FE328_RS01685"
                     /old_locus_tag="FE328_01670"
     CDS             complement(5001..7157)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /locus_tag="FE328_RS01685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01670"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_172763401.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.5e-41, bitscore:
                     138.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 2.1e-233, bitscore:
                     773.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASQFKWIKQQDTTDCAVACISMVLEHYGSKMPAHKLRNMTGTNR
                     RGTSALGMKNTFEKLKFNCNAVEADNNVWYHEDMIYPAIAHVVIKQRYQHYVVIYETDQ
                     SKLMIADPAEGLYTMTYEEFNNIWSGVLLLPQPTDQYKPVIEQVGGLTFFIPSLFRSKK
                     PVFSTIIASFLATSLSIVSSYYFQGVIDNILPEHNLSLLNITSLGLIGVYVFKVIFEFI
                     RRQLITILGQQLSKHIMLDYFNHVLKLPIKFFDTRKNGDIISRFLDANKIIDALASASL
                     ILFLDVAMVIIVGTFLFIQNNILFYITLVSLPVYFITAYIFVQKYDSANEKEMAAGSTL
                     NSGIIESLNGIETIKAYNGEKKIYENTCNNFLDFMEYSMKKVKLDNIQHIIKQGIQLLI
                     SAGVLWAGAYYIINGSMSIGQLITYNALLVFFTNPLESIINLQTQLQTAQVASKRLNEV
                     LSITSEESTSQDNNNHMTFDHQINIQNLTFSYDLKEPILNNINCRIRANQTVAIVGNSG
                     SGKSTLAKLMIKFYEPATGMIYYDNTPITDIKHSVVRENITYVPQDSFFFKGTILENLL
                     FGLSELPQEESIFHACEQAKIRKYIDQLPLGLKTPLEEGASNLSSGQKQRLAIARALLR
                     DTNIIIFDEATSNIDIFAENNIINSISQLSNKTIIHITHNLSIAKKCDSIFLIHEGTIK
                     EKGTHEELVANNHIYNKLWNTISE"
     gene            complement(7514..8785)
                     /locus_tag="FE328_RS01690"
                     /old_locus_tag="FE328_01675"
     CDS             complement(7514..8785)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636192.1"
                     /locus_tag="FE328_RS01690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01675"
                     /product="putative glycoside hydrolase"
                     /protein_id="WP_112788326.1"
                     /transl_table=11
                     /translation="MKSIKKTCPLLTALLFISSPVLAQQANEEPVEIKDRTPVEDMQAR
                     RDGLLNVYTESLIRLPKRIPQDLVYDSGVDIPYPEDGVKGIFVPGHSASDETTLNNLVD
                     FISSTDLNSIVIDVKEDYGNIVLDIEADGEFEEYINNALVADNDPAEILSLLDEHDIYP
                     IARVVTFKDTMLATERPDLSFREADGSVWKNGNGEAFVNPYEKEVWEYTLDIAKKAARL
                     GFKDIQFDYVRFPEGFEVFDETLTYSRGDYEDSDLTAGEQRMSAVNDFVKYAREELRPF
                     GVETSVDVFGYAAFIREAPGIGQSFLGISEHVDSISAMIYPSHWGPGNFNVPKPDLEPY
                     DTIDGYMELENELLAELEENAPRSRPWIQDFTASYLGAGNFKEYQAEDVTAQIQALYDN
                     GVEEYLIWNATGDYSRDAKFKITE"
     gene            complement(8786..10117)
                     /locus_tag="FE328_RS09365"
     CDS             complement(8786..10117)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF021854.4"
                     /locus_tag="FE328_RS09365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_112788325.1"
                     /transl_table=11
                     /translation="MRKKSVVTLASVALLLAACSGPSLEEQIQSTEETINQTASTFNQL
                     FEKEQTMMEIYETELETDATLENFNNESGALHENLVQRQKLIESIQDHNNDFEQAVNYF
                     TNYEGEDLSSEVANQFAEQLSTLHTQIVSYNDLYLQAINQQRQYFNSFAEEDMDYEHLS
                     YGVEVINETHERLYTQAQEINEQLEATVEQLDTLQHQEAIEPEADNTAANEGESATHDG
                     ATDKATAEPKYVLNEDLWTIEPIEDSTESQVALLTIDDAPYGNALDMAETMQEKGVSAI
                     FFVNGMYLDEDGRQVIQTLHDMGFEIGNHTQNHPNLSELTIDQQRNEIVETNDIIEETI
                     GIRPRFFRAPFGVMNADTYTVLDEEGMTAMNWTYGYDWEEQYQNPENLTNIMVNAPELN
                     HGANLLMHDRTWTRDALVDIIDGLAEKGYSFVNPSELTARKETE"
     gene            10242..10709
                     /gene="lspA"
                     /locus_tag="FE328_RS01700"
                     /old_locus_tag="FE328_01685"
     CDS             10242..10709
                     /EC_number="3.4.23.36"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="lspA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636190.1"
                     /locus_tag="FE328_RS01700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01685"
                     /product="signal peptidase II"
                     /protein_id="WP_112767922.1"
                     /transl_table=11
                     /translation="MIIYYIIAMILIGLDQLSKYLTVQEIALGEVVSLVPNVLSLTYIR
                     NSGAAWSILEDQMIFFYVITVVVVGALIYFLHTEGKKSPIASTGIAFIMGGAIGNFIDR
                     LHLKYVIDMIRLEFINFPIFNVADMALTIGVIILIGYIVYDELVKKKVKGS"
     gene            10709..11620
                     /locus_tag="FE328_RS01705"
                     /old_locus_tag="FE328_01690"
     CDS             10709..11620
                     /EC_number="5.4.99.-"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1279: pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636189.1"
                     /locus_tag="FE328_RS01705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01690"
                     /product="RluA family pseudouridine synthase"
                     /protein_id="WP_112788324.1"
                     /transl_table=11
                     /translation="MNKQQIELTIDQERGRLDKVLSNLIAEVSRNQIQQLIAEGAVLVN
                     GEKQPNKYKVASGDQVQVTIPEPEEIEAKPQNIPLEIVYEDEDIAVVNKPQGMVVHPAL
                     GHPDGTLVNALLYHLDNLSGINGKIRPGIVHRIDKDTSGLLMVAKNDQAHHHLSAQLKE
                     KTSKREYIALVHGRIPHEKATIDAPIGRDPHDRKKYAVVDDGKEAITHFNVLQRFKADY
                     TLIACQLETGRTHQIRVHLKYINFPMAGDPEYGPSKTLAGNGQFLHAATLGFIHPRTEE
                     YMEFNCPLPDIFTQTIAELEKS"
     gene            11744..12106
                     /gene="rplS"
                     /locus_tag="FE328_RS01710"
                     /old_locus_tag="FE328_01695"
     CDS             11744..12106
                     /GO_component="GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262406.1"
                     /locus_tag="FE328_RS01710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_01695"
                     /product="50S ribosomal protein L19"
                     /protein_id="WP_004636188.1"
                     /transl_table=11
                     /translation="MNPLIEAITQEQLRNDIPDFRPGDTVRVHARIVEGERERIQLFEG
                     VVIKRRGQGISETYTVRKISSGVGVERTFPVHSPRVAKIEVVRQGHVRRAKLYYLRNLT
                     GKAARIREKIRKEAKA"
ORIGIN
        1 ttggcgtatt tatgacgata caggcttttt aaactatgta ggagggttac ctgccggctc
       61 acaacgaaaa gctaatttac atgccttata tgagcgtgct gctgtttatg aagcatccag
      121 ttttaaaggg ttatttcact ttattcgctt tattgaaaaa atgaaagcac aagataaaga
      181 cttggccgaa gcgtcacata ttaccgatga agacaacgcc gttcgggtga tgacgattca
      241 tgggagcaaa gggctcgaat ttcccgctgt atttgtctgt aatttagcac ggcgacataa
      301 tatttctgat gcgcaaggcg cctatacctt agagcaatca ttcggattcg ggaccgatta
      361 tatcgatgtt actcaacata taagataccc aacctggatg aatcaagggc tcagcattcg
      421 gaagaaaagc caattactct ctgaacaaat gcgagtgctt tatgtggctt taacccgggc
      481 ggaacaaaaa ttattcttag tggggagcta taaaacaaaa gaaaaagcca ttgaaacttg
      541 gcagcaagca cgacgacacg aagacttgat tctgcccgat tatttacgga caacaggaca
      601 aacaactttc ttagactgga ttggctattg tttaatccgt catcgttcct tcaataacca
      661 cgaactccta gaagcgccac ttgataaatc aggcaaccac accatcgaac agtttgcggc
      721 taactttgat atcacgttca aaaatgaaca ggatttactt agccaaccgg ttgaagaagt
      781 gaagcacaaa gaacaaccgg tcgaactaac ccaggtatcg ttgcatgatt caaccatcca
      841 acaggcgatc gatcgaatga tgacacccta tacgcatcaa tcctccactg agacaactag
      901 ttatcaatcg gtctcagaat tgaagcgatt attcgaagta cccgaagatg ggcaaatggt
      961 taagttagat atctcagcac agaagccatc gattaatcgc tatgttcaag atgaactagc
     1021 agatccgagc tttatacaag cacgtaagtc gcaaccgact gcaagcgata tcgggcaagt
     1081 gacgcattta ctgcttcagt caatggactt aactaacgag ataacactag agactgtatt
     1141 ggcgcagtta acgcaattgg caggtaatca tcaattagat gccactacga ctaaacaagt
     1201 gccgattcaa tctattgtcc aatttttcca aactgatttt ggccaggagt tattgcaaca
     1261 gggtgaccga ctccagacag aaaaaccatt ctcgctgttg atggctgccc gtgatatttt
     1321 cgaacaaatt gatgtagtcg atgattatgt attgattcac ggaattgtgg atggctattt
     1381 tgaaacagaa gaagggatcg tcctatacga ctacaagact gatcgaatca gctatctcgg
     1441 tgaacaaaaa gcaaaagccg aattacgaca aaaatacagc ggtcagctaa acttatacaa
     1501 acaagccctg gaagttacct tgaataaacc tgttgtccga gcggttatca tctcacttga
     1561 cttagtagaa gcgattgact taatttaaat ataattgatt agaagtgcca atataaaaaa
     1621 caagcatgca gtctgcctaa tatgaaggct actgtctgct tgttttatta tataatgctc
     1681 acccatgcta tcttcttgat agtcatattt atgttaagca ttatgattgt ctaaaaatac
     1741 ttgttaattt aaaaattgaa aaagactata tccaaaatct tttcctgttt tataaattac
     1801 cggccatgcg tataatacca taatcccaag aatcgcatat aatgtgtaat gaatgatttt
     1861 tttgttttcc tgatagaaat cggtcaactt catgagtgat tctctccctt tgaatactaa
     1921 ataccttagg cgttagctcc ctccatgtat ccatcaatag caccaagccc aaaagcagca
     1981 gcagctactc caccaataat tagcaaagga cctccaccga taatagtggc taattctgtg
     2041 tttttaagtt ttttatattt agtcattgtt aataatctcc tttttagttg ataaaatcat
     2101 ataagcttct tccaaaatct tttccagtgt tataaatagc agtccaggca tcgcctaatc
     2161 cttcaagtac cccaccgcca ttaattttca ttaaatctgc tgtcaatatt cttctcatat
     2221 gtgtatttct cctaagcatt ttttgaattg atagttaatt ccagtcaatt atcttaatgt
     2281 ttttattgaa tgtggacaaa tttttaataa gggctgtcca ttaaatttga tagccgtatt
     2341 tatgttgcaa agaatactag ataaattccc tcctctaata taaaaaagtt ttttcttacc
     2401 tacttgtttc ataaaacaat ccctccttcc attaatttct taaaaaatgg gagtatacag
     2461 aatatttctt ttttaatttt ttcttgttcc ctcccattaa ataaagtata tcactcagat
     2521 agaaaaatag tgataaaatg cttagaaagg gaaaatgagt acttaaaata gtacaattta
     2581 aaaatgaaga atacaaaatc ttctgatgtt ataattttaa atttactcaa aaatttcttg
     2641 ttccggcatc aaattattca ctttaggctt tttaataaga aaaattgaaa aaaagtggta
     2701 taatttattt attgaaaaag ttagttaaga ggataaaata tgaaaatttt gaaaaaaagt
     2761 gctttaataa aaattatagg tggcgggaca atgaaagggc attttgaaaa tatgattaag
     2821 tgtgtgcatt ctttttttaa aaattgttaa ataatagata ctaagacatg ttgtatatgg
     2881 tgggagataa taaatgttta ctgaaactat tattgtatta attgaatata tttttaaagt
     2941 aatcactata tttatatttt cgatttggtt aataaaaccc aatactaact gtgttaaatt
     3001 aatatttggg agcctattcc ttccgatatt ttggtatgta attatagtat ttgaacaata
     3061 tttttatagt agtacgctgg gagaattact ttttgtattt ttttatacta tttctattca
     3121 gaagaaggag acaacgcaaa aataaaccag ttattttcgt ttattctaat taatgcatgg
     3181 attagttttt tattagcagt tccttttaat ttatttttag gtcaagagtg gtatggaagt
     3241 ttattggtag ccatttgttt acagattata gctattacag tttcaattta tatagtttat
     3301 aaaatcagaa aatatattac tatagtatca gaagacaagg ataatgaaaa aaagataacg
     3361 atgttgattg tagtaatgaa tttaataact atactttatt ttatgtacat aaagtataca
     3421 ggtcagtatc attcactatt aatattaaca acattttttt tagcggtgtt ctttttattt
     3481 atggggggac tatgttttta cttaatacaa tttattaaat ctaaacaaga aattagattt
     3541 ataaataaac aattacaaag taccaaggaa tacaataaga ttattgaaaa taatcaactc
     3601 aaattacgac aattcaaaca tgactacaaa aatattttga catcactaaa tggcttagtt
     3661 gcttcggaac aatatgatca attagaaatt aaattgaacc aactaactca gtactcaaaa
     3721 caagagttga aaaatgaaaa atcaatgtat gatgaattaa taaatgtaga gcatgagcta
     3781 ttaaagagta tcttgacggt taaattaatt gaaatggata acgaacaact taactttcaa
     3841 tttagttgtc ctaaaaaaat cactactata ggaatgcatt cttttgattt aatacgtgta
     3901 gtaggtattt taatagataa tgcaagggaa tatgcaatgg attataaggg aagtgagata
     3961 gaggttattg taaatagagg ggatgattat attgacatta taattgcaaa tacttttttt
     4021 gaaaatcatg catcaattga caggtttaag aaaaaaggat ataccacaaa attggggcat
     4081 aaagggttag gattaacaaa ggtggaagaa attaatcatc aatatgataa tgtattggta
     4141 aattatcgta ttgatgatta tttctcagtg gaaataacca ttctagatag agaaggagac
     4201 tagaaaatgt ttccaatttt tatttgtgaa gattctaaag aacagttaga tagattaata
     4261 acaatcgtca aatattatat tatggatcga gacaattatt atttagaaaa agttagcaca
     4321 aatcccgaaa aacttttatc ttatgttaaa gataaaagta taaaaaaagg tatctatctg
     4381 ttagatgtta atttaaatca tcatctaaca ggcattgatc tagcagaaaa gattagaaaa
     4441 caagatccgc atgcaaaaat tatttttatt accggacatg atgaatcaat gccagaaatg
     4501 ctaaaaagaa atattgaagt attaggattt gttcaaaaaa atgatgaatt agatatttta
     4561 agaaagaatg tcttgcaagc tatttcagca gcaaatgaac gattaattag tactctaacg
     4621 gaagataaag atatttttac atttagtttt ggagcaaaaa catttagatt tgattttaaa
     4681 gatgtattgt ctgttgaaac gacaggtaaa tcacatcaat tgataattta cttaactaac
     4741 ggacaatatg aaataagagg tacattgtct gaattagaga aaaagtatgc acaatttttt
     4801 agacttaatc gatcggtact aataaatcat cacaatgttg tacgcattaa ttattctgaa
     4861 agaattatta ttatgcgaaa tggaaccaca atctttttta ctaaacatca aacaaaaaaa
     4921 ttgaagaaat attttggata atgatgtgac cctctaaagt aatttttact agttacctta
     4981 gagggtcatt ttctgttcag ttattctgat atcgtattcc ataatttatt ataaatgtga
     5041 ttgttagcta ccaattcttc atgtgttccc ttttctttga ttgtaccttc atgtatgagg
     5101 aaaatactgt cgcatttttt agcaattgat aaattatgtg taatatgaat aatggttttg
     5161 tttgataatt gagaaataga attgataata ttattttctg caaatatatc aatattactc
     5221 gttgcttcat caaaaattat tatattggta tcacgtagta gtgctctggc aatagctagt
     5281 ctctgttttt gaccactaga taaatttgaa gccccttctt cgagtggagt tttcaaccca
     5341 agtggtaatt ggtctatata tttccttatt ttagcttgct cgcatgcatg gaaaatagat
     5401 tcttcttgtg gaagttctga tagaccaaat agtaagtttt ctaagatagt acccttaaag
     5461 aagaaggaat cctgtggcac atatgttata ttttcccgaa caacactatg cttaatatct
     5521 gtaattggtg tattgtcata ataaatcata ccagtagcgg gctcataaaa tttaatcatt
     5581 aattttgcta gtgttgattt accagagcca ctatttccta ctatagcaac tgtttgatta
     5641 gcacggattc gacaattgat attattaagt atcggttctt ttaaatcata cgagaaagtt
     5701 aaattttgaa tattaatttg gtgatcaaag gtcatgtgat tattattatc ttgagatgta
     5761 ctttcttctg atgtaatgga tagtacttca tttaatcgct tgcttgctac ttgggctgtt
     5821 tgtaattgag tttgtaagtt aatgatactc tcaagtggat tagtgaaaaa tacaagtagt
     5881 gcattataag taatcaactg tccaatactc attgatccat taataatata atacgcacct
     5941 gcccacagta cacccgcact aatgaggagt tgaatgcctt gtttaattat atgttgaata
     6001 ttatctaatt tgactttttt cattgaatac tccatgaaat ccagaaaatt attacaggtg
     6061 ttttcataaa tttttttttc accgttgtaa gctttaattg tctcaatacc atttagactc
     6121 tctataatac ctgaattaag tgtcgaacca gcggccattt ctttttcatt tgcactatca
     6181 tatttttgta caaaaatgta tgcagtaatg aaataaacag ggagagagac tagggtgatg
     6241 tagaataata tgttattttg gataaataag aatgttccca cgataataac catagcaaca
     6301 tcaagaaata atattagaga tgcgctagca agtgcatcaa taatcttatt ggcatctaag
     6361 aatctagata taatatcacc atttttacgc gtgtcaaaga actttatagg caatttaagt
     6421 acatgattaa agtagtctag catgatatgc ttactcagct gttgaccaag aattgttatt
     6481 agttgacgcc taataaattc aaatataacc ttaaacacgt atacacctat taaaccaagt
     6541 gatgttatat tgagtaaaga taaattatgc tcgggcaata tattatcaat tacaccttgg
     6601 aagtagtatg agctaacgat actaaggcta gtagctaaaa aagatgcaat aatagtagag
     6661 aagacaggtt tttttgatcg gaatagagag ggaatgaaaa atgttaatcc accgacttgc
     6721 tcaataacag gtttatattg atccgttggt tgagggagaa gtagcacacc agaccaaata
     6781 ttattaaatt cttcataagt cattgtgtac agtccttcag caggatcagc aatcattaac
     6841 ttagattgat cagtttcata aatcacaaca taatgctggt atctttgttt tataacaaca
     6901 tgtgcaatcg ccggataaat catatcttca tgataccaaa cgttattgtc agcttctaca
     6961 gcattacagt taaactttaa tttctcgaat gtatttttca tacctaaagc tgatgttcct
     7021 ctacgattag ttccagtcat attacgtaat ttatgcgccg gcattttaga tccataatgt
     7081 tcaagaacca tacttataca ggcaaccgca caatctgttg tgtcttgttg ttttatccat
     7141 ttgaattgac tagccattat tgaaactcct ttgtattatg ataatactag tatctcataa
     7201 ttggagagtc attcgataaa aggtctctaa attggtattt tgagtgcgtg aaatgttatt
     7261 gagagattca tcgatggtta gttataccat tgtgaaacca tagataaaca ctttcaatga
     7321 cgaacagaaa ttttataaaa aagatcgaaa tagatagaag taatctaaca ttatagtact
     7381 gttgttgatg gctactgata tatatttaaa ataaaaacca tttgtaaggt tactacttgc
     7441 tggataggat aaccagtcaa tagcaacctt acaaatggtc aatttagtag tcttaataaa
     7501 attttgacag tacttattct gttattttaa acttagcatc tcgagagtaa tcaccagttg
     7561 cattccaaat caagtattct tcaacaccat tgtcatagag ggcttgaatt tgggcagtga
     7621 catcctcagc ttgatactct ttaaagttgc cagcccctaa gtagctagct gtaaagtctt
     7681 gaatccacgg acgagagcgc ggagcatttt cttctaactc agccagtaat tcattttcaa
     7741 gttccatata gccatcaatt gtatcatatg gttctaagtc aggctttggt acgttgaagt
     7801 tccccgggcc ccagtgactt ggataaatca tcgctgaaat actgtccaca tgttcagaaa
     7861 tgcctaagaa actctgacca atgcccggtg cttcacgaat gaatgctgca tatccaaaga
     7921 catcgactga cgtttcgacg ccaaatggtc taagttcttc gcgagcatac ttaacgaaat
     7981 cattaaccgc tgacatacgt tgttcaccgg cagttaaatc agaatcttca tagtcaccac
     8041 gactataagt gagtgtctca tcaaagactt caaacccttc tggaaagcga acataatcaa
     8101 attggatatc tttaaagcct agacgagctg ctttcttggc aatatcaagt gtgtattccc
     8161 aaacttcttt ttcataggga ttgacgaagg cttctccgtt gccatttttc caaactgatc
     8221 catctgcttc acggaaggag agatcaggtc gttctgtagc aagcatggta tctttgaagg
     8281 taacgacccg agcaatcgga taaatatcgt gttcgtctag taaacttaaa atttcagctg
     8341 gatcgttatc agcgacaagg gcattattaa tatattcttc gaattcaccg tctgcttcga
     8401 tatccaggac aatattaccg taatcttctt tcacatcgat aacaattgag ttcagatcag
     8461 tagaagagat gaagtccact aaattattta gtgttgtctc atctgaagcg ctatgtccgg
     8521 gaacaaaaat acctttcaca ccatcttcgg gataaggaat atccacacca gagtcataga
     8581 ctaaatcttg agggattcgc ttcggcaaac gaataaggga ttccgtatag acgttaagta
     8641 agccatctct gcgggcttgc atatcttcga caggtgtccg gtctttgatt tcaacgggtt
     8701 cttcattagc ttgttgtgct aaaacagggg aactaataaa gaggagtgcc gttaatagag
     8761 ggcatgtttt tttgattgat ttcatttatt cagtctcctt acgtgcggtc agttcactcg
     8821 ggttaacgaa tgaataacct ttttctgcta aaccgtcaat aatatcaact aaagcatcgc
     8881 gtgtccatgt gcgatcatgc atgagaaggt tagctccatg atttaattcg ggtgcattga
     8941 ccataatatt ggttaagttt tcaggatttt ggtactgttc ttcccaatca taaccgtatg
     9001 tccagttcat cgctgtcata ccttcttcat cgagaacagt ataggtatct gcattcatca
     9061 caccaaatgg cgctcggaag aatcgtgggc gtataccaat tgtctcttcg atgatgtcat
     9121 tggtctcaac aatttcatta cgctgttgat cgattgttaa ctcagataaa tttgggtgat
     9181 tttgggtatg attcccaatc tcaaagccca tatcgtgtaa tgtctgaata acttgacggc
     9241 catcttcatc aagatacatg ccattgacaa agaagatagc agagacgcct ttttcttgca
     9301 tcgtttcggc catatcgaga gcattaccat acggagcgtc gtcaattgtc agtaaagcaa
     9361 cttgagattc ggtgctgtct tcaattggct caatcgtcca taagtcttcg tttaagacat
     9421 atttaggttc agcagtagct ttgtcagtag ctccatcatg tgtagcagat tctccttcat
     9481 tagcagctgt attgtcagct tcgggctcaa tcgcttcctg atgttggagg gtatctaact
     9541 gttcaacagt agcttcgagt tgctcattaa tctcttgagc ttgtgtatag agtcgttcat
     9601 gtgtctcatt aatcacctcg actccgtatg ataaatgctc gtaatccata tcttcttcag
     9661 caaatgaatt aaaatattga cgctgttgat taattgcttg taagtataaa tcattataag
     9721 atacaatttg ggtgtgtaag gttgataact gctcagcaaa ttgattggct acctcagatg
     9781 ataaatcttc tccttcataa ttagtaaaat aattgaccgc ttgttcgaag tcattattat
     9841 ggtcttggat ggattcaatc aatttttggc gttgaactag attctcatgg agtgctcccg
     9901 attcattgtt gaaattttct agtgtggcat ccgtttctag ttcagtctca taaatctcca
     9961 tcattgtctg ttctttttcg aataattgat taaatgttga agcagtttgg ttaattgttt
    10021 cttctgtaga ttgtatctgc tcttccaatg atggccctga gcaggcagct agcaacaagg
    10081 cgacactggc taatgtaact actgattttt ttctcattca gatctcctcc ataaataagt
    10141 cctatactca ctataacagt ttttgagaga aaattgtagt ttaaatagaa aatttatcaa
    10201 aattgctgta agatagaaag taaacgaaaa gaggtcagga aatgattatt tattatatta
    10261 ttgctatgat attgattgga ttggatcagt tgagcaaata tttaactgtg caagaaattg
    10321 ccttgggaga agttgtgtcg ctcgtgccta atgtgctgtc gctgacatat attcgcaata
    10381 gcggagcagc ctggagtatc ttggaagatc aaatgatttt cttctatgtg ataactgttg
    10441 tcgtcgtggg cgcacttatt tacttcttgc acacagaagg gaaaaaatcc ccaattgcta
    10501 gtacaggtat tgcttttatt atgggcggag cgattggaaa ttttattgat cggttgcatt
    10561 taaaatacgt tatcgatatg attcgtttag aatttatcaa ttttcccatt ttcaatgtgg
    10621 cagatatggc gctaacgatt ggcgtaatta ttttaattgg ttatattgtc tatgatgaac
    10681 ttgtaaagaa gaaagttaag ggatcctaat gaataaacaa cagattgaac ttaccattga
    10741 tcaagaacgc gggcgcctgg ataaggtgct aagcaattta atcgctgagg tctcgcggaa
    10801 tcagattcag cagttaatcg cagaaggtgc tgtgttggta aatggagaaa aacaaccgaa
    10861 taaatataaa gtagctagtg gagatcaggt gcaggtaacg attcctgaac cagaagaaat
    10921 agaagctaaa ccacaaaata taccgcttga aattgtttat gaagatgaag atatcgctgt
    10981 agtaaacaaa ccccaaggca tggttgttca tccggcacta gggcatccag atgggacctt
    11041 agtgaatgcc ttattgtatc atttagataa cttatccggt attaacggaa aaattagacc
    11101 tggtatcgtg catcggattg ataaagatac atccggacta ttgatggtgg ctaaaaatga
    11161 ccaggcccat catcacttat cagcacagct aaaagagaaa acctccaaac gagagtatat
    11221 tgcacttgta catggacgta ttcctcatga gaaagcaacg attgatgccc caattggccg
    11281 tgacccacat gaccgaaaaa agtatgccgt agtggatgac ggtaaagaag ctattacgca
    11341 ttttaatgtc ctgcagcgct ttaaagcaga ctataccctg attgcatgcc aacttgaaac
    11401 cggacgtaca catcaaattc gcgtgcactt gaaatacatt aatttcccta tggcgggtga
    11461 tcccgagtat ggtccgagca agacgttagc agggaacggg caatttttac atgcagcaac
    11521 actcggcttc attcatccta gaacagaaga gtatatggag tttaactgtc cattgccgga
    11581 tattttcacc cagacaattg ctgaattgga aaaatcgtaa aaaattcttt acatttagaa
    11641 gaaaatatga tagtatatat aattggtggg ggaccaccgt aatcatgcaa ttatccgcta
    11701 ggacgtatgc acccatgata attgcgcgag aggagtagaa agaatgaacc cattaattga
    11761 agcgatcaca caagaacaat tacgtaacga tattcctgat tttcgtccag gagatactgt
    11821 acgtgttcac gctcgaattg tagaaggaga gcgcgaacgt attcagttat tcgaaggtgt
    11881 tgtcatcaaa cgccgtggtc aaggaattag cgaaacatac actgttcgca aaatttcaag
    11941 cggtgttggt gttgaacgta ctttccctgt acattcacca cgtgttgcga agattgaagt
    12001 tgttcgccaa ggtcacgtac gtcgtgctaa attgtactac ttacgtaact taactggtaa
    12061 agcagcgcgt attcgtgaga aaatcagaaa agaagcaaaa gcataattct gaattaaaga
    12121 gctttctgtt taggcagaag gctcttttgt cgtctaa
//
