LOCUS       NZ_CP040417            22499 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3077 chromosome.
ACCESSION   NZ_CP040417
VERSION     NZ_CP040417.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1957024)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 351x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655765.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:25:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,806
            Genes (coding)                    :: 1,779
            CDSs (with protein)               :: 1,779
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 12 of 27
            Pseudo Genes (incomplete)         :: 15 of 27
            Pseudo Genes (internal stop)      :: 6 of 27
            Pseudo Genes (multiple problems)  :: 6 of 27
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:08:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 153557
            Orig. end                         :: 176056
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..22499
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..22499
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22499
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12499](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12499
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    complement(250..259)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.44"
                     /tool="antismash"
     misc_feature    complement(729..746)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.05"
                     /tool="antismash"
     gene            complement(833..1300)
                     /locus_tag="FE328_RS00705"
                     /old_locus_tag="FE328_00705"
     CDS             complement(833..1300)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636451.1"
                     /locus_tag="FE328_RS00705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00705"
                     /product="universal stress protein"
                     /protein_id="WP_111951578.1"
                     /transl_table=11
                     /translation="MLQEYQRILVAVDGSESANRALRKAVAVAKRNDATLFITHVIDTR
                     AFQPYEAFDASVSKSAKSEANSTLNACKLYAENHGLSDVHLLLEHGSPKKLIARDLPNS
                     YDIDLIMLGATGLNTVERFFIGSVSENVVRSALCDVLVVRTDTENNYEIGH"
     ncRNA           complement(1418..1600)
                     /db_xref="RFAM:RF00013"
                     /gene="ssrS"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="FE328_RS00710"
                     /ncRNA_class="other"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="FE328_00710"
                     /product="6S RNA"
     gene            complement(1418..1600)
                     /gene="ssrS"
                     /locus_tag="FE328_RS00710"
                     /old_locus_tag="FE328_00710"
     gene            complement(1701..2966)
                     /locus_tag="FE328_RS00715"
                     /old_locus_tag="FE328_00715"
     CDS             complement(1701..2966)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636450.1"
                     /locus_tag="FE328_RS00715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00715"
                     /product="replication-associated recombination protein A"
                     /protein_id="WP_208958067.1"
                     /transl_table=11
                     /translation="MKQPLAYRMRPESIDEVVGQQHLVGEGKIIWRMVEAKRLSSMILY
                     GPPGIGKTSIASAIAGSTKYAFRMLNAATDSKSDLNVVVEEAKMSGTVILLLDEIHRLN
                     KPKQDFLLPHLESGRIIMIGATTENPYLSIHPAIRSRSQIFELNPLQEADIISALNRAL
                     DDDTNGLGHLELTVDEAALKHFARATNGDLRSSLNALELAAESTTPEDGTIHITLPIAE
                     ECIGRQALTHDKDGDAHYDVISAFQKSIRGSDVDASLHYLARLMVAGELEVACRRLMVT
                     AYEDIGLANPAGAARAVTAVQAALRLGLPEARIPLANAVVELALSPKSNTAYKALDQAI
                     SDIREGKAGEVPKHLKDTHYSGADSLGRGQGYKYPHNYDNAWVKQDYLPDRLAKAQYYM
                     PKTNSKFETALSRQFEKFNTDQ"
     gene            complement(3011..3994)
                     /locus_tag="FE328_RS00720"
                     /old_locus_tag="FE328_00720"
     CDS             complement(3011..3994)
                     /EC_number="6.3.4.15"
                     /GO_function="GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /locus_tag="FE328_RS00720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00720"
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="WP_208958068.1"
                     /transl_table=11
                     /translation="MHSHLIFQLNKQFPNPVPIETLSQQFNCSAAEITEAVEELRALGL
                     VIHSYNSGFQLASPLYSIPGIKTYLNTQTFGTQGLYLFETIDSTHTYVSNNLSTIDHGS
                     VLLAYSQTAGRGRRGRKWSSPIGKSLSLSIVLKEFSAQMKPTLLTQLTAAALQQALSET
                     GLPVAIKWPNDLLINRRKVAGVLTEAMFERQVLQAITIGIGINLNQSQTDFPTEIHTKA
                     TSLALETESPFFADKLIAQFLKIFEHFYQDYQQSLDPEPFLSICRQESAVIGESVTVVQ
                     GDKRRDVEVLGIEATGELLVRDLSSQEQMTLISSEVSLRNPDGSYI"
     gene            4116..5798
                     /locus_tag="FE328_RS00725"
                     /old_locus_tag="FE328_00725"
     CDS             4116..5798
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636448.1"
                     /locus_tag="FE328_RS00725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00725"
                     /product="ribonuclease J"
                     /protein_id="WP_077862255.1"
                     /transl_table=11
                     /translation="MSNVKIIPLGGVRENSKSLYVVEVDAQIFVLDCGLMFPEGELYGI
                     DAVIPDFTYLKQNKERIAGVFLSHGHEDAIGALPYFIDEFNVPVFGSELTIELARLQVE
                     SVIGKGNFSDYHVVDSSLAIDFGEVKVHFFQVTHTIPDAMGISIETPEGHVVYTGNFKF
                     DQSADGPYRTNLNHISDIGKERVLALLSSSLGAESLEENAPDKRVQSVVTEIFQDAPTR
                     IIVAAVQSNLLRIQQIINAAHATNRKVFISSSKSQDIINVAIDLGKLTLPKKDILQSIS
                     DLEKFHDDETVILETGSSGEPIKALREMATNQHPNIGIRNEDTVFIATNPSVAMEVEVA
                     ECENLIYRSGGEVVKLSNQIKASGHATPNDLQLMMNLLHPEYFIPVQGEYRVLAAHARL
                     AHETGIPHEHIFTLSNGDVVEYKKGKMHSTGTVPADNTLVDGIGIGDVGNIVLRDRRLL
                     SNDGIFVAVVTIDRRKQKIVAKPHIVTRGFVFVKANYDLINESTTIVEKVIQQNLKHND
                     FDWGSIKSEIRNELSDYLFKETKRRPIILPVIMEVNQRRWYDK"
     gene            5892..7346
                     /gene="cls"
                     /locus_tag="FE328_RS00730"
                     /old_locus_tag="FE328_00730"
     CDS             5892..7346
                     /GO_function="GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cls"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636447.1"
                     /locus_tag="FE328_RS00730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00730"
                     /product="cardiolipin synthase"
                     /protein_id="WP_208958069.1"
                     /transl_table=11
                     /translation="MSYISTIFWWFIIINSTLAIFTIFRDKTRDIASIWAWLLALIMLP
                     GIGFIAYLFFGRGMNDKDIYDIKQQDQIGLRELKDTLTERYEHQHEAGDEVFSNNKKEM
                     VSLFQGLDNAALTRDNEVDIYSDGKEKFDQLLEDIKQAKHHIHLVYYIFRGDKIGRAIV
                     DALEEKANQGVEVRVLYDPVGTRWMTRSFFKKLKSFGGQACPSFGERMHILNMRLNYRN
                     HRKIVVIDGKIGYTGGFNVGDDYLGEYPEMGYWRDTHVRIVGNGVLPLQTRFLTDWNAS
                     ADEAQQVPYDNQYFPIQETTGDVDLQIVSSGPDSTEQQIKKGFIKMISLARKSVYIQTP
                     YLVPDDAVLETIDIASKSGVEVHVMIPNKPDHPFIYRATLSYAEQLVEYGAHVHIYDGG
                     FLHAKTIIVDDEMLSIGTANFDIRSFKLNFEVNTFMYSEKLAQAYHAQYMSDLQHAYEL
                     TPDIIANYSFWERFKQQFARLFSPIL"
     gene            complement(7437..7790)
                     /locus_tag="FE328_RS00735"
                     /old_locus_tag="FE328_00735"
     CDS             complement(7437..7790)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636446.1"
                     /locus_tag="FE328_RS00735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00735"
                     /product="DUF2200 domain-containing protein"
                     /protein_id="WP_077862257.1"
                     /transl_table=11
                     /translation="MGRHKIFDMAVSAVYPHYVNKAEKKGKTAEQVDELMCWLTGYSQD
                     ELHEQLEANVSFEKFFDQAPKLHPNRTLIKGVICGKRVEDIEDPLMQNIRYLDKLIDEL
                     ARGKKFENIKRTQ"
     misc_feature    7831..7847
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 25.02"
                     /tool="antismash"
     gene            7983..8396
                     /locus_tag="FE328_RS00740"
                     /old_locus_tag="FE328_00740"
     CDS             7983..8396
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS00740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_00740"
                     /product="hypothetical protein"
                     /protein_id="WP_142970426.1"
                     /transl_table=11
                     /translation="MNPESQHQNLQLQLQETITHLDKITDQLESAKRWSWADLLGGEFI
                     TSMVKRKKMKNISEDLQQLQPKLQHLKHALEDANIQVDSAIDHSTSRQFFDTFFDNIIT
                     DLKVQGEIKDMLAELKELRQALTTYQQQIDSGV"
     gene            8619..8816
                     /locus_tag="FE328_RS00745"
                     /old_locus_tag="FE328_00745"
     CDS             8619..8816
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF016486.4"
                     /locus_tag="FE328_RS00745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00745"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_142970427.1"
                     /sec_met_domain="L_biotic_A (E-value: 7.1e-10, bitscore:
                     35.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQKQSVAIETEQLELVQAVSDERLEVLVGGKGPGLLKTLTKDCP
                     GTVSDVCVNLAVVSTCKKCE"
     CDS_motif       8619..8711
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEQKQSVAIETEQLELVQAVSDERLEVLVGG"
                     /locus_tag="FE328_RS00745_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       8712..8816
                     /prepeptide="core"
                     /locus_tag="FE328_RS00745_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="0.20"
                     /molecular_weight="3400.0"
                     /monoisotopic_mass="3397.8"
                     /core_sequence="KGPGLLKTLTKDCPGTVSDVCVNLAVVSTCKKCE"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEQKQSVAIETEQLELVQAVSDERLEVLVGG"
                     /leader_location="[8618:8711](+)"
                     /alternative_weights="3418.1"
                     /alternative_weights="3436.1"
     gene            8913..9335
                     /locus_tag="FE328_RS00750"
                     /old_locus_tag="FE328_00750"
     CDS             8913..9335
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /locus_tag="FE328_RS00750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00750"
                     /product="ABC transporter permease"
                     /protein_id="WP_208958070.1"
                     /transl_table=11
                     /translation="MKTLLKKEFNTHGQNKLLYIVLLVVGIMQVFLARSLFGAEGGGAL
                     AKTIFQDNSIIYSLVATIFTPIIAGVLGYYFISSEFEQRTWEMYFLGIKNKSKILWTKY
                     VVCLFYWIAYNICVWGIFLGLSAFYWQRQVDLFLFG"
     gene            9713..9892
                     /locus_tag="FE328_RS00755"
                     /old_locus_tag="FE328_00755"
     CDS             9713..9892
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294876.1"
                     /locus_tag="FE328_RS00755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00755"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_142970428.1"
                     /sec_met_domain="L_biotic_A (E-value: 2e-11, bitscore:
                     40.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKYNDENKKAQKTVEVIKDKELEQMIGGVGPGLVRTLTKDCPNV
                     VPKVCFDGNCKYCR"
     CDS_motif       9713..9799
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKYNDENKKAQKTVEVIKDKELEQMIGG"
                     /locus_tag="FE328_RS00755_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       9800..9892
                     /prepeptide="core"
                     /locus_tag="FE328_RS00755_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.30"
                     /molecular_weight="3246.8"
                     /monoisotopic_mass="3244.6"
                     /core_sequence="VGPGLVRTLTKDCPNVVPKVCFDGNCKYCR"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKYNDENKKAQKTVEVIKDKELEQMIGG"
                     /leader_location="[9712:9799](+)"
     gene            10001..12499
                     /locus_tag="FE328_RS00760"
                     /old_locus_tag="FE328_00760"
     CDS             10001..12499
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024964.4"
                     /locus_tag="FE328_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00760"
                     /product="DUF4135 domain-containing protein"
                     /protein_id="WP_208955440.1"
                     /sec_met_domain="DUF4135 (E-value: 1.8e-58, bitscore:
                     195.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 6.3e-25, bitscore:
                     84.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEDKQLINEKNSFDQVIDEITTYYFQQIKIVNIFNVNTIKQQLLD
                     EVRKLYLPALVDDINHYRLNVDESSTPEEQYMTYCRLFNREKLTELHTKYPLLDKWVHN
                     TVERYKTNLKFLSDAIAIDYDEIVKVFNLSQEAKDNIEVMSFVGDVHHGSQAIQFKLAG
                     KEFYLKRNGQLVLELVEKLKETIIASSQFDFPKTYLGEQYFIQEAVQQEPCQSEGDIAD
                     FYFNMGMLLSFVYMLNGSDMHNENVIASGKTPYVVDIETLINPVDFSNDSCIEQSIFQT
                     AMLPMKYNPTFEGIVDCSSIGEVVTVRKKIFEAENPFSSRIRLKLKDVYYEDIATHLPL
                     YDSEYHSVGSYLHKVEEGFTVGYRTIIGNKKNLINIVEQYSVAVQCRLVMRNTIVYSEL
                     LKRLNSPDLLVSISQSRQFLHHFLWDIRYITQNKQEYINEEINQLLEGEVPYFTYGNEG
                     TILSKDVESLASIKESLLMKIEQFSEEDLSFQLDLLRVAFNIDNYNFTRLHMSHLKESV
                     NDLIHESIYDETILTIQKDPKGNYVYNAIGLGVYEGQLGLWLTDKCFHRHISYDDVIRD
                     IINSKDIGGINGYASLCVCQRLLGLPLSNVMIDETKIAQIDVIDGLAGLILVLYSDYRE
                     SPSKSKLTQLINLFELFEKNYNVDEINRLGFAHGISGIKIIFYIMSQLLDTTFRDNYRE
                     IDKLDNSEDYLSATWCNGLTGHLIAEYILYKISGDANHMNNVTKQLPKLVQLAYNMDDY
                     CLCHGLFGVLDFLLTVKREGKLSSDMIRKYYELERYAYRYLDEHDVLSKDISLFTGLGG
                     ISYYLNRKESDIKSVLMFGY"
     gene            12519..14564
                     /locus_tag="FE328_RS00765"
                     /old_locus_tag="FE328_00765"
     CDS             12519..14564
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="FE328_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00765"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_142970430.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.9e-33, bitscore:
                     111.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHVPEIIQFNQNDCGPCCLSMLLNYYGYESDIEELTELKSAIYG
                     WSMLDIKRIGEKFGLESNVYNVQTIQQLKQVKLPAILFWEHSHFVVLEKIIADEIIIVN
                     PSVGRQTVNKKVFWEKFTGYIMEVSLGQSFQKKKIPWYDKVQLYMNKDIWNFKIKWQYV
                     ALLIVYSALLFISPLAVSEVISSVGLDHIPYGKIMALVGVVIATGITNYLLDKRRQREK
                     LDLEESSTLKLYNEISKKSKVEFSKYHSGDTLSRLHVNSDICQFITIGIPNMLLSIGLF
                     MVVTIYLLIRVGMMSLILSGVILLIALINGLFIVPLMKMAQTESYKFSDFRTVLSDGII
                     SHNYFINSGIGGTYTEKVTHSLKDYLNVSYDRSKIEAKSNTVQQMTSTFFSLILSVLGM
                     GLILIFPNDRSNISLLMTTGIILYSPAMSVLSTILTVSRVAPHMKRLNEMIKNNQQNYT
                     TKRLKDGSIKVKDLTFQYENTADPIINDRSFRLNHGEKLVICGESGSGKTTLIDLLLGL
                     NKQYEKGEVMIGGVNTKEYDLNLKEDVCYITQPTKLFKGTLKDNLKLYLDHYDVEQLIE
                     ITADMELDTLGRECDLSNHLILEDGLNFSQGQKQRLGLLRLFLGKYKIIIVDEPTSNLD
                     DRLARKIFSMIDELNATKIIITHDEKYIQQADKVLNLGDDEHEYQV"
     gene            14548..15456
                     /locus_tag="FE328_RS00770"
                     /old_locus_tag="FE328_00770"
     CDS             14548..15456
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636443.1"
                     /locus_tag="FE328_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00770"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_208958071.1"
                     /transl_table=11
                     /translation="MNIKFNNVNKTYGRKQVLQDINITIPEGKIYGFIGANGAGKTTAM
                     KILTGLTPASSGTVTFDGKELNQLENKAKAFGAFISTPTYYKNLTAYENLAIIQDVLEE
                     PKDEIDRVLEIVGLADAKSKTIAEYSFGMKQRLGLAFSFLNDPDILILDEPTNGLDPKG
                     IVEIRELLYSLSKEQGKTIFISSHNISELESIADMIGIIQNGKLIFEGELDELYASGES
                     SYLLEIDDIDQAKTVLSEEGISFTNKEHKFQIKSSKQRIPEVVKALLDRDIAIFEITPN
                     KNLERIFLDLTDGDDGHVDTH"
     gene            15440..16165
                     /locus_tag="FE328_RS00775"
                     /old_locus_tag="FE328_00775"
     CDS             15440..16165
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /locus_tag="FE328_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00775"
                     /product="ABC transporter permease"
                     /protein_id="WP_181453070.1"
                     /transl_table=11
                     /translation="MWTLIKHEFKTHGTQPILYLILGIMGLLQVFLTTIMIKTTDTVSI
                     QGSYIQTVFQTNNAIFLSNSIIFIFSVGAVIGYYIISVEYQNNTWEMLLLGTGSKSKVL
                     WAKYIVSTLYYLSYQVLFYSMFLLVQSTYFNLQIEISFSLLMLVSIMFLSLVLFTAQIA
                     CHYLIKNGTTAIACAVGFLIMLVILPSTDLFRYVIRLLTPGYLAGLDEFSVTGFVSVVA
                     LNIIVASSMMSLVVKQFKL"
     gene            16178..16876
                     /locus_tag="FE328_RS00780"
                     /old_locus_tag="FE328_00780"
     CDS             16178..16876
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_00780"
                     /product="hypothetical protein"
                     /protein_id="WP_208958072.1"
                     /transl_table=11
                     /translation="MFLKLELLKFKRRISVVALCLFMLLVVGLQTFIAMKTPELETNVF
                     YIGSLEWFSGIIFPALAPVFITLSVYADTEYKGIQNFVLKGVKPQKLSQSTWKFFLVAL
                     PLLYVSYVMIPFVFMFFRGGLTSEGIWSVVIYSGLSVISVVSLVNISFIIYRITMNDFL
                     PVIIAVAGVMLAFAPFGQDLWLVNPYLYMYYSSAHSGFNWYHYAVIIGLCAVSFAVYAY
                     VSYYKKTKRA"
     gene            16881..18365
                     /locus_tag="FE328_RS00785"
                     /old_locus_tag="FE328_00785"
     CDS             16881..18365
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS00785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_00785"
                     /product="hypothetical protein"
                     /protein_id="WP_185908145.1"
                     /transl_table=11
                     /translation="MEKWTPKIDVIYHIVFSVLAVYLFLGYKGVSYLPLMMSLWLLLLL
                     FDYMMMTIIGVRTQIARLIVWTFRLAQVQSISLMTTIQLPLVIRYVLLMMLLNLLCQCI
                     TSLLTQYVDNRLLMRRLGIGLVVGIGLWTDYANILFIAYIVYVLFSTILAIKQRINHHV
                     YTKNKHTGYWVLVAAFSLQLIPLLGLVILVVLSSQLFTQVWLFFMAVASTLLMLIAGFN
                     HKAVVGQASKWLSLIFCTLLYIYIFVLILGLNLSLVLFILYIIVSIMYILTLTDVFGNH
                     QVNAVRLIKREEALKQDFSNYLHDDVLQDINVLIKMTTLEPSEKTQAFLREQLSALNDQ
                     LRQQMNQYSPQLSKHLTLQENYRLLVRSLEQTYPNQAVSTSFSMNRNLTLPGPYDVLVY
                     RWLRELIHNVYKHAGASQLDIHVAAYDELIIVLVEDDGCFKKGSHLEIGHGLYVIQEQV
                     EAIGGQLYIEANRPRGLRMRVEFSVMGGEIIEDFVN"
     gene            18346..18945
                     /locus_tag="FE328_RS00790"
                     /old_locus_tag="FE328_00790"
     CDS             18346..18945
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636437.1"
                     /locus_tag="FE328_RS00790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00790"
                     /product="response regulator transcription factor"
                     /protein_id="WP_142970435.1"
                     /transl_table=11
                     /translation="MKILLIDDHKLFSQSLALVLDQTTSDVQVDMINSEEELPDDLGEL
                     TVYDVLVLDINLDKGFGCDGFELAERVRAIAVDLPILMLTGFDLPVYEYQAHKLELSGF
                     VNKNIDTEDLLSILKHVKDGGRHFTTENWFIDELTPRERELLGAIATGRKRKIIAEEMY
                     ISERTLTNHMQSIMDKLEVNSTIEAIQKARELGYLK"
     CDS             18942..19052
                     /gene="allorf_0172499_0172609"
                     /locus_tag="allorf_0172499_0172609"
                     /protein_id="allorf_0172499_0172609"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MIGGGRKMTFGVYGSCALNDCLIYSCIALNWGVNWG"
     CDS_motif       18942..18974
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MIGGGRKMTFG"
                     /locus_tag="allorf_0172499_0172609_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       18975..19052
                     /prepeptide="core"
                     /locus_tag="allorf_0172499_0172609_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.80"
                     /molecular_weight="2699.1"
                     /monoisotopic_mass="2697.2"
                     /core_sequence="VYGSCALNDCLIYSCIALNWGVNWG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MIGGGRKMTFG"
                     /leader_location="[18941:18974](+)"
     gene            19073..19255
                     /locus_tag="FE328_RS00795"
                     /old_locus_tag="FE328_00795"
     CDS             19073..19255
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE328_RS00795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE328_00795"
                     /product="hypothetical protein"
                     /protein_id="WP_111974584.1"
                     /transl_table=11
                     /translation="MMKYAPYSLLALAGILVYLTVNRYITMHSINVVPPIGAILCVIFF
                     FVWRNHLKQQGYYDD"
     gene            19613..22273
                     /gene="polA"
                     /locus_tag="FE328_RS00800"
                     /old_locus_tag="FE328_00800"
     CDS             19613..22273
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="polA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622796.1"
                     /locus_tag="FE328_RS00800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE328_00800"
                     /product="DNA polymerase I"
                     /protein_id="WP_201711049.1"
                     /transl_table=11
                     /translation="MSKKKLLLIDGYSIAFRAFYAMHSQLHRMKNKNGLHTNALYGFHN
                     MLEAVMEKEQPTHALVAFDAGKTTFRHELFDDYKGGRESMPAELSEQIPYLKELIAAYG
                     LQTYQLPDYEADDIIGTLSTQASDEFEVVIITGDKDLTQLASDQTRVDITKKGITQLKS
                     YTPESMMEEMGIAPEQITDLKGLSGDSSDNIPGVTQIGDKTALKLLKKFHTVEELYERI
                     DEMNASKRKEYLIAEKETALLSKQLATIITDAPIEISPESLAYSGKNMEALIGFYKEMD
                     FNSHLEKLDTDVYFEELQDEAEAVEYEWVEEVTEAMFGQSGTLYFEMFEENYHTAPYII
                     ASWTNEKKVYVAEPEMLLASKDFQDWLEDESARKYTYDAKALMVSLKQRELEIANITFD
                     LSLASYVLTAENSSGGDVAAIANKHGYVDVLEDEAVYGKGKKVSIPNDKTVMYEHVARK
                     VTAIDALAEQLTVELADNDQLALFEEIELPLAHVLGEMEIQGITTDANRLETMKEEFAD
                     VLEEIEAKIYAEAGESFNINSPKQLSDVLFSEDKLNYEPIRKTKTGYSTAQDVLEKMTS
                     YAPIAKLILEYRTIAKLQSTYVEGLLDVIEADGKIHTRYGQTVARTGRLSSVDPNLQNI
                     PVRSEMGRKIRQAFVPREDGWKIFGADYSQIELRILAHMSEDEALIETYLNDQDVHTTT
                     AMKVFDLESPAEVTANQRRDAKAVNFGIVYGISDYGLSENLGITRKEAREYIDTYFSRF
                     PNVKEYIDQSIREAKDKGYVETLYHRRRYLPDINSRNFNVRSFAERTAMNTPIQGSAAD
                     IIKVAMVEMQKRLKASDLKATMLLQVHDELIFEAPESELAELEALVSDVMEHVVELDVP
                     LQVASSSGDNWYEAK"
ORIGIN
        1 gaggggcttt ttcttgccaa gcggtttccc aagcttcaag aactttggcg cgttcttcag
       61 gagtctcagc ggatgttagt aagttatcag cttgtgctgc agagtactct tttagctcac
      121 catcgcgttc aattttaacg gtaacagaag cgactaaatc atcatacata tcgctccacc
      181 cattaaatcc gtcaacggat aattgattga tcgctgattc tagatctaag ctgagtagtt
      241 ctttggcctg gtcgcgttct tcatttaaac ggaaagcaat ggactggaag ggttcatctt
      301 cgagtagagc ttgccaatca tcattactga tagtttctat ttttttcgtc aattcagttg
      361 agtaattgct gaattgagtg gcaagtaagc ttaatttgtt aatgtgaata ggagcatgct
      421 ggtcatcttg cttagctgat agatgcgcca tcgagaatgt aaagccttgt gataggccgt
      481 tagagatgtc atcagataag ttgaaaatag ttgctagttc ttcagcatga accgcgcctt
      541 cttctggggt ccattttttt accaattcac cgaaacggtc aatatcagtt tgtaatgctt
      601 caattttttc ttgtaattca ggagaattac taccgcctga aaagacggat tctaaatccc
      661 aaacttgttc ataagtcata attatcatcc tttcgtatgt tcattattgt acactaagcc
      721 tatgttaata tccactttaa gggttgttaa ctacatggtt ttataaaaaa gcagccagcc
      781 atcacaaaaa gtgagacagt tagctgctta aatgatgaat ctataatatc gattagtgtc
      841 caatttcata attgttttcc gtatcagttc gaacgacaag gacatcacaa agagcagaac
      901 ggacgacatt ttcggagacg gaaccgatga agaagcgctc aacagtgttt agtccggttg
      961 cgccaagcat aattaaatca atatcataac tatttggcaa gtcacgggcg atcaacttct
     1021 tcggtgagcc gtgttctaag aggagatgta catcactcag tccatggttt tcggcatata
     1081 acttgcatgc atttaaggta gagttagctt cggatttcgc tgatttggag acgctagcgt
     1141 cgaaggcttc atagggctgg aaggcgcgtg tatcaattac atgagtaata aataacgtgg
     1201 catcattccg tttggcgacc gcaaccgctt ttcgcaatgc acggttagca ctctcagacc
     1261 catcaactgc gacaagtatt cgttgatatt cttgcaacat ttgtctcatt cctttcactt
     1321 aattgttatg tacagtatac caaaaatcaa acggtttcac aaaagataat aataaaaagc
     1381 cactgatata agataatcag tgaccatttt tatgtacgag gtatccactg gtgccgatcc
     1441 gttgtctgaa aggttgtacc cgctatttgc aggtgggctt ctccactcac gcatctgacg
     1501 tccaagtgaa aaggcttgtc atgaacatga gctttcgaat cccaatgtat aaatgttcgg
     1561 tcaactcgtt aaatagacaa ctcgaacacc tcagatacct cacttatata ttagcatgtc
     1621 agtgaaaaaa tgtcaattgc tctcaccaat tctttcaaat cggaagcatc ctctatgtga
     1681 ctacatattt aaatagcaca ttattgatcc gtgttaaatt tctcaaattg tcgagaaagg
     1741 gcggtttcaa atttgctatt tgtttttggc atgtaatatt gtgctttagc aaggcgatca
     1801 ggtaaataat cttgcttaac ccacgcatta tcatagttat gaggatattt gtatccttgg
     1861 ccgcgtccga gtgaatcggc tcctgaataa tgagtatctt ttaagtgttt gggaacttca
     1921 ccggcttttc cttctctaat atcactaata gcttgatcca gtgctttata agctgtatta
     1981 gatttaggag agagggcaag ttcgacgacc gcattggcta gtggaattcg agcttcaggt
     2041 aagcctaggc ggagagctgc ttgaacagca gtcactgctc gggcagcacc agctggatta
     2101 gctaagccaa tatcttcata agctgtcacc attaagcggc gacaggctac ttctagctca
     2161 ccagcgacca ttagccgagc taaataatgg agcgatgcat ccacatcact gccacgaatt
     2221 gatttttgga aggcggagat aacatcatag tgagcatccc catccttatc atgggtgagt
     2281 gcttggcgac caatacattc ttcagcgatt ggcaaggtaa tatgaatcgt tccgtcttcg
     2341 ggagtagttg attcagccgc caattctaat gcattcaagc tacttcttaa gtcaccgttc
     2401 gttgcacggg cgaagtgttt gagtgctgct tcatccactg ttagttccaa atgccctaat
     2461 ccattcgtgt catcatctag agcacggttt agcgctgaga taatatcagc ttcttgcaag
     2521 gggttgagtt caaaaatctg acttcgactt cgaatagccg ggtgaatcga taagtatgga
     2581 ttttccgttg ttgctccaat cataataata cgcccactct ccaagtgtgg caggagaaaa
     2641 tcttgttttg gcttgtttaa gcgatggatt tcatcaagaa gcaaaataac agtcccactc
     2701 attttggctt cttctacgac cacatttaag tcagatttgg agtcagtggc tgcattcagc
     2761 attcggaagg catatttggt tgaacccgca atggcactgg cgatgctggt tttaccaata
     2821 cctgggggac catagagaat catcgagctc agacgcttgg cttccaccat tcgccaaatt
     2881 attttacctt caccgacaag atgttgctgt ccgacgactt catcaataga ttcgggtcgc
     2941 atgcgataag ctaagggttg tttcatctga attatccttt cttagatagt cataaatcaa
     3001 ggtttaaaag ttatatataa ctaccatcgg gattgcgcaa ggatacttca gaactgatca
     3061 atgtcatctg ttcttgcgaa gagagatcgc gtacgagaag ttctccggtt gcttcaatgc
     3121 ctaacacctc aacatctcgg cgcttatctc cctgaacaac agtgacactt tcgccaataa
     3181 ccgctgattc ttgccgacaa atagataaaa agggttctgg atcgagggat tgctggtagt
     3241 cttgatagaa gtgttcaaaa atcttcaaaa actgtgcaat taatttatct gcaaaaaaag
     3301 gggactctgt ttctaaggcg agtgatgttg cttttgtatg gatttcagtg gggaagtctg
     3361 tctgagactg attcaggtta atcccaatac cgattgtaat agcttgtaaa acttgacgtt
     3421 caaacatggc ttcagttaaa acgcctgcta cttttcgtcg gttgataagt aaatcattgg
     3481 gccattttat tgcgacaggt agaccggttt ctgacagagc ttgttgcaga gcagcagccg
     3541 tcagttgcgt taagagagtc ggtttcatct gagcagagaa ctccttcaac acgatgctga
     3601 gagagagact tttaccaatg ggactggacc actttcgccc gcgacgtcca cgcccagctg
     3661 tctgactgta tgctaagaga acactgccat gatcgatggt cgataagtta ttactaacat
     3721 atgtatgcgt agaatcaatc gtttcaaaga gatataaccc ctgagtgccg aaagtttgtg
     3781 tgttgagata ggtcttaatg ccagggatgg aatagagagg cgaagctaat tggaagccac
     3841 tattgtatga atgaatgacg agacctagcg ctcgcaattc ttccaccgct tcggtaatct
     3901 cagcagcaga acaattgaat tgttgactta gtgtttctat tgggacgggg ttagggaatt
     3961 gtttatttaa ttgaaaaata agatgagaat gcataagaac tccttctaaa ttatgaaaat
     4021 tagtcagtct atgctataat tataaggacg aaatgaactg tagtcaatga atatgagaat
     4081 atagataaaa gtagatcaaa taaaggagaa ttattgtgtc aaatgttaaa attatcccct
     4141 taggtggggt tcgtgagaat agtaaaagtt tgtatgtggt ggaggtggac gcccaaatat
     4201 tcgtattaga ctgtggattg atgttcccag aaggtgaatt atacggcatc gatgcggtga
     4261 ttcctgactt cacttattta aagcaaaata aagagcgcat tgccggtgtg tttttatcac
     4321 acggtcatga ggatgcgata ggagcgctcc cgtactttat cgatgaattc aatgtgcctg
     4381 tattcgggtc cgagttgacg attgagctgg ctcgcttaca agttgaatca gtgattggga
     4441 aagggaactt ctctgattat catgttgtag attccagtct ggcgattgac ttcggtgagg
     4501 tgaaggtgca tttcttccaa gtgacgcaca cgattcctga tgcaatggga atctctattg
     4561 aaacaccaga aggacatgtt gtttatacgg ggaactttaa gtttgatcag agtgcagatg
     4621 gtccgtaccg tacgaactta aaccatattt cagatattgg gaaagaacgc gttctagcgt
     4681 tgcttagctc atcactagga gcggaaagtc ttgaagagaa tgcaccagat aaacgagttc
     4741 aaagtgttgt gacagagatc ttccaagatg cgccgactcg tattattgta gcagctgtcc
     4801 agagtaactt actgcgcatc cagcaaatca ttaatgcagc ccacgcgacg aatcgtaaag
     4861 tctttatctc tagctcgaaa tcccaagata ttattaatgt agccattgac ttaggaaaac
     4921 taacattgcc gaaaaaagac atcttacaat ctatatcgga cttggagaag ttccacgatg
     4981 atgagacggt tatcttggaa actggctcaa gtggagaacc gattaaagct ttacgcgaga
     5041 tggcgactaa tcaacatcct aacattggta ttcgtaatga agatactgtc tttatcgcga
     5101 caaacccatc agtagcgatg gaagtggaag tagctgaatg tgagaacttg atttatcgtt
     5161 caggaggcga agtggtcaag ttatctaacc aaattaaggc aagtggacat gcaacaccga
     5221 atgatctgca attgatgatg aacttacttc acccggaata ctttattccc gttcaaggag
     5281 agtaccgcgt cttagccgcc cacgctcgct tagcacatga gacaggtatt ccacacgaac
     5341 atatttttac tttaagcaat ggggatgttg tggagtacaa aaaaggcaaa atgcacagta
     5401 ccggaacggt tccggctgac aatactttag ttgacggtat tggtattggc gatgtgggca
     5461 atattgtctt acgtgatcgt cgcttactgt ctaacgacgg aatcttcgtg gctgtcgtaa
     5521 cgattgaccg tcgcaaacaa aagattgttg ccaagcccca cattgttaca cgaggctttg
     5581 tctttgtgaa agctaattat gaccttatta atgaatcgac tacaattgtg gaaaaagtga
     5641 tccaacaaaa cttgaagcat aatgatttcg attggggttc gattaagtca gagattcgca
     5701 atgaattgag cgattatctc tttaaagaaa caaaacggcg cccaattatc ttaccggtta
     5761 ttatggaagt gaaccaaaga cgctggtatg ataaataatt gaggactgtt tgtatcctat
     5821 aaaatatagt caaagggaga gcggagcttc taagttagct ctgctcttat ctacataaaa
     5881 aggagattaa tatgtcatat atctcaacta ttttttggtg gtttataatc atcaactcga
     5941 ccttagctat ctttaccatc tttcgtgaca agacccgtga tatagcctca atttgggcat
     6001 ggcttttagc ccttattatg ctgccgggaa ttggatttat tgcttatttg ttcttcggtc
     6061 gaggaatgaa tgataaagat atttacgata ttaagcaaca ggatcagatc ggattacgcg
     6121 aattgaaaga caccttaacc gaacgatacg aacatcaaca tgaagcaggg gatgaagtat
     6181 tttctaataa taaaaaagaa atggtttctc tgtttcaagg attggataat gcagcactaa
     6241 cacgtgataa tgaagttgat atttattcgg atggtaagga aaagttcgat cagttactgg
     6301 aagatattaa gcaagccaag catcatattc atctcgttta ttatattttt cgaggggata
     6361 agattggtcg tgccattgtg gatgcactgg aagagaaggc taatcaaggt gtcgaagtac
     6421 gtgttctata cgatccggtg ggtactcggt ggatgacgcg atcctttttt aagaaattaa
     6481 aatcattcgg tgggcaagcc tgtccatcat tcggtgaacg gatgcatatt ttaaatatgc
     6541 gcttgaacta tcgtaatcac agaaaaatag ttgtaattga tggaaagatt ggctacactg
     6601 ggggcttcaa tgtcggggat gattacttag gagaatatcc ggaaatgggt tattggcgcg
     6661 atacgcatgt ccgaattgtc ggtaatgggg tactcccatt gcagacccgc tttttaaccg
     6721 attggaatgc atcagctgat gaggcacaac aagttcctta cgataaccag tattttccta
     6781 ttcaagagac aacaggtgat gtcgatttgc aaattgtctc tagcggtccg gatagcaccg
     6841 aacaacagat caaaaaaggc tttattaaga tgatcagctt agcgcgtaaa tctgtttata
     6901 tccagacccc ttacttagtg ccggatgatg ctgtgttaga gacgattgat atcgcctcta
     6961 agtcaggggt agaggttcat gtcatgattc caaacaaacc ggatcatccc tttatttatc
     7021 gggcaacgtt gagttatgcc gagcagttag ttgagtacgg cgctcatgtg catatttacg
     7081 atgggggctt cttgcatgcg aagactataa ttgtagatga tgagatgtta agtatcggaa
     7141 cagctaattt cgatatccgt agcttcaagt tgaacttcga agttaacacc ttcatgtata
     7201 gtgaaaaact agctcaagct tatcatgctc aatatatgag tgacttacaa cacgcctatg
     7261 aattaacacc cgatattatt gctaactatt cattctggga acggttcaag caacaattcg
     7321 ctcgcttatt cagtcctatc ttataaggta taataaaact ccgtatcatc attatggttt
     7381 cgatgagtac ggagttttca gtatgtataa acaaggatga ccccctgttg atggtatcat
     7441 tgggtacgtt tgatattttc aaattttttg cctcgtgcaa gttcatctat taatttgtcc
     7501 aagtagcgaa tattttgcat tagcggatct tcgatatctt caacacgttt cccacagata
     7561 actcctttga ttaatgtgcg atttggatgt aacttaggcg cttggtcgaa gaatttttcg
     7621 aatgatacat tagcctccaa ctgttcatgc aactcatctt ggctgtagcc tgtcagccaa
     7681 cacatcagct catccacttg ctcagcagtc ttgcccttct tttcagcctt gttgacataa
     7741 tgcggatata ccgcactcac cgccatatca aaaattttat gtcgtcccat aatgttacca
     7801 tctcctttct ttctattcta acatacgaaa ctgacataat ataacattga gctttctaaa
     7861 ctaacatttg tttcctaaat tagaagtatt tagtcgagtt gccgaaatta ctcatagatt
     7921 tagttgagtg atgtggtaaa ataaggctag agatagcaac gacagacaag gagatgctta
     7981 acatgaaccc agaatcacaa caccaaaacc tacaattgca actgcaggag acaatcactc
     8041 acctggataa gattaccgat caactggaat cagccaaaag atggtcgtgg gcggatctgc
     8101 tagggggtga atttatcacg agtatggtaa agcgtaagaa aatgaaaaac attagcgaag
     8161 acttacagca actccaacca aaactacagc acttgaagca cgcactggaa gatgccaata
     8221 ttcaagttga tagcgccatt gatcactcaa catcgcgtca gtttttcgat acgttcttcg
     8281 ataacatcat caccgacttg aaagtacaag gcgagatcaa agacatgctg gcagaactta
     8341 aagaactccg ccaagcattg acgacgtatc aacagcagat tgatagtggt gtctaattta
     8401 gaaaatatct aaaagtagct agaaaaaagc tagttgcttt ttgtgactca atcattgatt
     8461 aaaattccaa aagtatggtc cagtagaaga atataacatt gcctaattta ctcatgagcg
     8521 ctgatcgatg tatggggtag aatataactg taataaaagg aaatgcacgg tggcgattat
     8581 tgtaagaaaa aacaataaac ccaaaggagt cggtgtaaat ggaacaaaaa caaagcgtgg
     8641 caattgagac agaacagtta gagttggttc aagcagtatc tgatgagagg ttagaagtgt
     8701 tggtaggtgg gaaaggcccc ggactgttaa agacacttac aaaagactgt cctggtacag
     8761 tttccgatgt ttgtgtaaat ttagcagttg ttagcacctg taaaaaatgt gagtaaatag
     8821 agataaaaat gatatcgtgc ataatcccac cagtaatatg gtgggtttcc ttttttgata
     8881 tgcagaaaga attatacaga aaggactaaa aaatgaaaac actactaaaa aaagaattca
     8941 atacacacgg gcaaaataaa ttattgtata ttgttctact agtcgttggc ataatgcaag
     9001 tgtttcttgc gagatcgtta tttggagcag aagggggagg agcactagct aaaacgatat
     9061 ttcaggacaa cagcatcatc tattctctag tagctacgat atttacgccg ataatcgcgg
     9121 gagttttggg ttattatttc atttcaagcg agttcgaaca aagaacttgg gagatgtatt
     9181 ttttaggcat aaaaaacaag tcaaaaatct tatggacaaa gtatgtggta tgcttgttct
     9241 attggatagc atataatatt tgcgtttggg gaatatttct agggttatct gcattttatt
     9301 ggcagcgtca agttgacctt tttttgtttg gttgatccct attagcacta ctctattagg
     9361 actatttctg ttcacagcac aattgagtat gcatttttta atacccaata ctcaaatatc
     9421 aatttctata gtgatggtat ttattggaat tgttggaatg gttttagaat catggctaat
     9481 ttatgttttg ccattgtcat ccggatttta tttgaatcat atagatcaat ttaataattt
     9541 agagtatatg cggttaatta ttcaaaatat tattttttca atcatcttaa tatctattgt
     9601 agttaaaaga ttccatttat aattaccttt tttactcatg atttttttga aaagtcacta
     9661 taaaatatat acggagtcat cgaagtcaca caaaaagaaa ggttgatata tcatggaaaa
     9721 atacaatgat gaaaataaga aagcacagaa aactgtcgag gttatcaaag acaaagaatt
     9781 agagcaaatg atcggtggag taggaccggg acttgttaga acactaacaa aagattgtcc
     9841 taatgttgtt ccaaaggttt gttttgatgg aaattgtaag tattgtagat agagtaaagg
     9901 gttaacaatt aatcgatgac tttaggctct gtacaaataa gtgcagagca ttacatagaa
     9961 aaatgttcgt atgtaattgg atgttgaggg agtaatgata atggaagata aacaattgat
    10021 aaatgaaaaa aatagttttg atcaagttat tgatgaaatt acaacttatt atttccagca
    10081 gataaaaatt gtaaatatct ttaatgtcaa cacaattaaa cagcaattat tagatgaagt
    10141 tagaaagtta tatttacctg cattagttga tgatatcaat cattatcgat taaatgttga
    10201 tgaatcttct acgccagaag aacagtatat gacgtattgc aggttgttta atagagaaaa
    10261 attaactgag ttgcatacaa agtacccgtt actggataaa tgggtacaca atacggtcga
    10321 aagatataag actaatttaa aattcttatc agatgcaatc gcaattgatt atgatgagat
    10381 cgttaaagta tttaacttat ctcaagaggc aaaagacaat atagaagtca tgtcatttgt
    10441 tggagatgtg catcatggca gccaagcaat tcaatttaaa ttagctggca aggaatttta
    10501 tcttaaaaga aatgggcaat tagtactaga gctagttgaa aaattgaaag agactattat
    10561 agcatcaagt caatttgatt tccctaagac atacttaggt gaacaatatt ttatccaaga
    10621 agcggtgcaa caagaaccat gtcaatcaga aggagatatt gctgattttt atttcaatat
    10681 gggaatgttg ttgtcatttg tctatatgtt aaatggaagt gatatgcaca acgaaaatgt
    10741 aatagctagt ggtaaaacac cgtatgtcgt ggatattgaa acattgatta atccagttga
    10801 tttttccaat gattcttgca ttgaacaaag tatcttccaa acggctatgc ttccaatgaa
    10861 gtataatcca acatttgaag gaatagttga ttgtagttca attggtgaag ttgtcacagt
    10921 aaggaagaaa atttttgaag cggaaaatcc atttagttca cgtattagat taaaactgaa
    10981 agatgtgtac tatgaggata ttgcaactca tctaccattg tatgatagtg aataccatag
    11041 tgttggtagt tatttgcata aagtagaaga gggatttaca gttggatata gaactattat
    11101 aggcaacaag aaaaacctaa taaatatcgt cgaacaatat agtgtagcag ttcaatgtcg
    11161 attagtgatg cggaatacca tagtttatag tgaattattg aagcgactta attctccaga
    11221 cttattggta tctatcagcc aatctcgaca atttctgcat cactttttat gggatattcg
    11281 ctacatcacc caaaataaac aagaatatat taatgaagaa attaatcagt tgttagaggg
    11341 agaagtacca tattttacgt atgggaacga aggaactatc ctgtcaaaag acgttgagtc
    11401 acttgcttca ataaaagaat cactgctaat gaagatcgaa caattttctg aagaagattt
    11461 gtcgtttcaa ttagatttac tgagagtagc attcaatata gataattaca attttacacg
    11521 tttgcatatg agtcatctga aagaatctgt gaatgacctt attcatgaat cgatatacga
    11581 tgagacgata ttgactattc agaaagatcc gaaaggtaat tatgtctata atgctattgg
    11641 gttaggcgta tatgaagggc agttaggact atggctcact gataaatgtt tccatcgaca
    11701 tatttcttat gatgatgtta taagagacat tattaacagt aaagatattg gtggaatcaa
    11761 tgggtatgca tcattatgtg tatgccaacg actattaggt cttccattat caaatgtaat
    11821 gattgatgag acaaagattg ctcaaataga tgtgattgat ggtttagcag ggttaatttt
    11881 ggtactttat agcgactaca gagaatctcc atcaaaaagc aagttgactc aattgattaa
    11941 tctatttgaa ctatttgaaa agaactataa tgttgatgag attaatcgtt taggatttgc
    12001 ccatggtata tcggggataa aaattatctt ctatattatg agtcaattgc tagatacaac
    12061 ttttagagac aattacagag aaattgataa gttagataat tcagaagact acttatctgc
    12121 aacgtggtgc aatggcttga caggacattt aatagctgag tatattctgt ataaaatatc
    12181 tggtgatgca aaccacatga ataatgtgac taagcaacta cctaaactag ttcaattagc
    12241 atataacatg gatgattatt gcttgtgtca tgggctattt ggagtgttag actttttatt
    12301 aacagtgaaa agagaaggaa aactatcatc agatatgatt aggaaatatt acgaattaga
    12361 aagatatgca tacaggtatt tagatgaaca tgatgtattg agtaaggata tttcactatt
    12421 tacaggattg ggaggaatca gttattacct taatcgcaaa gagtctgata tcaaatcggt
    12481 gttaatgttt ggctactaaa agaaaggagc ttgcggttat gaagcatgtg ccagaaatta
    12541 tccagtttaa tcaaaatgat tgtggtccat gttgtttgtc catgttactc aattattatg
    12601 gttatgagtc agatatagaa gaattaactg aattgaaatc agcaatatat ggttggagca
    12661 tgttggatat aaagagaatt ggagaaaaat ttggattaga aagtaatgtt tacaatgtgc
    12721 agacaattca acaattaaaa caagtgaaac tgccagctat tttgttttgg gaacattctc
    12781 atttcgttgt gttagagaag ataatagctg atgaaataat cattgttaac ccaagtgtgg
    12841 gcagacaaac agtcaacaaa aaagtatttt gggaaaagtt cacgggctat attatggaag
    12901 tttcgttagg gcaatcattt caaaagaaaa agattccttg gtatgataaa gtacagttat
    12961 atatgaataa agacatatgg aattttaaga taaaatggca gtatgtagca ttacttattg
    13021 tatatagtgc attgttattt atatcaccat tagctgttag cgaagtaatt agttcagtgg
    13081 ggctagatca tattccgtat gggaaaataa tggcattagt tggtgtagtt attgcgacag
    13141 gtatcactaa ttatttgtta gataagagaa gacaacgtga aaaattagac ttagaagaat
    13201 catcgacttt gaaattatat aacgaaatat caaaaaaatc gaaagtagaa ttttcgaaat
    13261 atcatagtgg cgatacttta tctagattac atgtaaacag tgatatttgt caatttatta
    13321 cgataggtat ccctaatatg ttactatcaa tcggattatt tatggtagta acaatctatt
    13381 tattgattag agtaggaatg atgtcactta ttttaagtgg agttatttta ctaatcgcat
    13441 taatcaatgg gttgttcatt gtaccattga tgaagatggc tcagactgag agttacaaat
    13501 tttcggattt tcgaacggta ttatcggatg ggatcatatc gcataattac ttcatcaatt
    13561 caggaattgg cggaacatat acggagaaag taactcatag tttgaaagat tatctcaatg
    13621 tttcttatga tcggtcaaag atagaggcaa aatcaaatac tgtgcaacaa atgaccagta
    13681 cgttcttttc attgatatta tcggttttgg gaatggggct tatccttata ttccctaacg
    13741 atcgcagtaa tatatcattg ttaatgacaa cgggaataat tctttattcc ccagcgatgt
    13801 ctgtgttatc aactatattg actgtatcga gagtagctcc gcatatgaag cgattaaatg
    13861 aaatgataaa aaataaccag caaaactata caactaagag attgaaagac ggttcaataa
    13921 aggtaaaaga cttaaccttt caatacgaga atacagcgga tcctattata aacgatagaa
    13981 gtttcagatt gaaccatgga gaaaaattag ttatttgtgg tgaatcgggc agtggaaaaa
    14041 cgactttaat tgatttgtta ttaggattaa ataaacagta cgaaaaaggt gaggttatga
    14101 taggaggtgt gaacacaaag gaatatgatt tgaatttaaa agaagatgtg tgctatatta
    14161 cccaaccaac gaaattgttt aaagggactt tgaaagataa tttaaaatta tacttagatc
    14221 attatgatgt tgaacaatta attgaaataa cagctgatat ggagttagac acattgggta
    14281 gagagtgtga tttatcaaat catctcatat tggaagacgg gcttaatttt tcgcaaggtc
    14341 aaaaacaacg cttaggctta ttgcgactgt ttcttgggaa gtacaaaata attattgtag
    14401 atgaaccaac aagtaatctt gatgatagat tagcacgaaa aattttcagt atgattgatg
    14461 agctaaatgc aaccaaaatt attattacac atgatgagaa gtatatacaa caagctgata
    14521 aagtactaaa tctaggagat gatgaacatg aatatcaagt ttaacaatgt caacaaaaca
    14581 tacgggcgta aacaggtctt gcaagatatt aacattacca ttccagaagg caagatttat
    14641 ggatttatcg gagcgaatgg tgcgggaaaa acaaccgcaa tgaagatttt gactggattg
    14701 acgccggcga gtagtgggac agtcactttt gatgggaagg aattaaatca gctagagaat
    14761 aaagcaaaag cgtttggtgc ctttatcagt acgccgacgt attacaagaa tctaacggcg
    14821 tatgaaaact tagcaattat ccaagatgtc ttagaagaac ccaaagatga gattgatcgc
    14881 gtactggaga ttgttgggtt ggccgatgcg aagagtaaaa cgattgctga gtactcattt
    14941 ggaatgaagc aacggctggg attagcgttc tccttcttga atgatccgga tatcttgatt
    15001 ttagatgagc caacgaatgg acttgacccc aaaggtattg tggagattcg agaattactg
    15061 tattcattgt cgaaagaaca aggcaagaca atttttatct cgagtcataa tatttcagag
    15121 ttggagtcga ttgctgatat gattggcatt atccaaaatg gtaaattgat ttttgaagga
    15181 gagcttgatg aattatacgc aagtggcgaa agttcttact tattggagat agacgacatc
    15241 gatcaagcta agacagtgct tagcgaagaa ggcatctcat ttaccaataa ggaacacaaa
    15301 ttccaaatta aatcgtctaa gcaacgtatc cctgaagtag ttaaagcatt gttagatcgt
    15361 gatattgcta tatttgagat aacaccgaac aagaacttag agcggatttt cttagattta
    15421 acggatggag atgacggaca tgtggacact cattaaacat gaatttaaga cacatggcac
    15481 tcaaccgatc ttgtatttaa tattaggaat tatgggatta ctacaagttt ttctcacaac
    15541 aatcatgatt aaaacaacag atactgtatc gatacagggg agttatattc aaacagtttt
    15601 tcaaacaaat aatgcgatat ttctcagcaa ctcaattatt tttattttca gtgtaggtgc
    15661 tgtgatagga tactatataa tatcagtcga gtatcagaat aacacatggg agatgttact
    15721 attaggaaca ggtagtaaat caaaagtatt atgggccaag tatatcgtat ctaccttata
    15781 ttatcttagt tatcaggtat tgttttattc aatgtttttg ttggtgcaat cgacatattt
    15841 caaccttcaa attgagatat cctttagcct attgatgcta gtatcgatta tgtttttatc
    15901 acttgtgcta tttacagcac agatagcgtg tcactactta attaaaaatg gcacaacggc
    15961 tattgcctgt gcagttggat tccttataat gcttgtgatt ttaccatcaa cagatttgtt
    16021 tagatatgtg atacggttat tgactccagg atatttagcg ggattagacg agtttagcgt
    16081 cacgggattt gtaagtgtcg ttgcattaaa catcatcgtt gcatctagca tgatgtcact
    16141 tgttgttaaa caatttaaat tataggaggc tgagcaaatg ttcttaaaac tggaattatt
    16201 gaaatttaaa cgaagaatta gtgttgttgc gttatgttta tttatgttgc tagttgtggg
    16261 acttcaaaca tttatagcaa tgaaaacacc agaattggag acgaatgtat tttatattgg
    16321 tagtttagaa tggtttagtg gaattatttt cccagcgttg gctcccgtgt ttattacgtt
    16381 atctgtttat gcagatacgg aatataaggg tatacaaaat tttgtgttaa aaggggtgaa
    16441 accccagaaa ttatctcaat cgacgtggaa attcttcctt gttgcgttac ccttgctgta
    16501 tgtaagttat gtgatgatac catttgtctt tatgttcttt agaggtgggt taacgagtga
    16561 aggaatttgg tctgttgtga tatacagtgg tttgtccgtc atcagcgttg tttcacttgt
    16621 gaacatcagt tttattatct acagaataac gatgaatgac tttttacctg tgattattgc
    16681 cgttgcgggt gtgatgttag cttttgcccc atttggacaa gatctgtggt tagttaatcc
    16741 gtatttatat atgtactaca gttctgcaca tagtgggttt aattggtatc attatgccgt
    16801 tatcatagga ctttgtgcgg tcagtttcgc cgtgtatgcg tatgtaagct attataaaaa
    16861 gacaaaaagg gcgtaatcat gtggaaaagt ggacacccaa aatagatgtt atttaccaca
    16921 ttgtattttc agtattagct gtgtatttgt ttttaggcta taagggagtg tcttatctcc
    16981 ctcttatgat gagtttatgg ctgttattac tactttttga ttatatgatg atgacgatta
    17041 taggcgtgcg tacgcagatt gcccgactga ttgtctggac attccgactc gcacaggtgc
    17101 aatcaatctc attaatgaca actattcaac tgcctctcgt gatcagatac gtgttattaa
    17161 tgatgctgtt gaatttatta tgccaatgta tcactagttt attaacgcaa tatgtcgata
    17221 atcgtttatt gatgagacgc ttagggattg gcttagttgt tggtatcggt ttatggacgg
    17281 attatgccaa tattttgttc attgcttata ttgtttatgt gctgttttca accattttgg
    17341 caatcaaaca gcgaattaat caccatgtgt atactaaaaa taaacacaca ggttattggg
    17401 tactcgtcgc ggcgttttcg ttgcaattaa ttccactact cggactagtt atattggtgg
    17461 tgttgtctag tcagttattt actcaggtat ggttattttt tatggctgtt gcttcaacac
    17521 ttcttatgct aattgctggc tttaatcata aagctgttgt gggtcaggca tcgaaatggt
    17581 tgagcttaat attctgtact ttactctata tttatatttt tgtgcttatt ttagggttaa
    17641 atctaagttt agtgttattt atattatata ttatcgtaag tattatgtat atattgacat
    17701 taactgacgt atttggcaat catcaagtga atgctgtcag gttaattaaa cgggaagagg
    17761 ctctaaaaca agatttctcc aattatttac acgatgatgt tttgcaagat attaatgtat
    17821 tgatcaagat gacaacgttg gagccgtctg agaagacaca agcgttcttg cgggagcaac
    17881 tgtcagcttt aaatgatcaa ttgcgccagc agatgaatca gtatagtcca caattatcca
    17941 agcacttaac tttgcaagag aactatcgtc tgcttgtgag gtcactggaa cagacgtatc
    18001 cgaatcaagc tgtgtcgaca tctttttcga tgaatcggaa tttgacttta cctggaccgt
    18061 atgatgtgtt ggtttatcgg tggctcaggg agttgattca taatgtgtac aagcatgcag
    18121 gagcgagtca gttggatatt catgtggcag cgtacgatga gttgataatt gtgttggtgg
    18181 aagatgatgg gtgctttaag aagggctcac atcttgagat tggtcatggg ttgtacgtga
    18241 tccaagagca agtggaagca attggtggcc aactgtatat agaggcgaat cgtccacggg
    18301 ggttgcggat gcgggttgag ttcagtgtga tgggaggcga gataattgaa gattttgtta
    18361 attgatgatc ataagttatt ttcgcaaagt ttggcattag ttctagatca gacaacaagt
    18421 gatgtgcaag tggatatgat taacagtgaa gaggagttgc ctgatgatct aggcgagttg
    18481 acagtgtatg atgtgttagt gttggatatt aatttagaca agggattcgg gtgtgacggg
    18541 tttgagttgg cagagcgggt gcgtgctata gcggtagatt tgccaatttt gatgttgact
    18601 ggttttgatt tgccggtcta tgaatatcag gcgcataaat tggaattaag tggatttgtc
    18661 aataaaaata ttgataccga agatttattg agcatactca agcatgtgaa agatggtggg
    18721 cggcatttta caacggagaa ttggtttatt gatgagttga caccacgcga gcgagagttg
    18781 ttaggggcta tcgcgactgg gaggaagagg aaaataattg ctgaagaaat gtatatttct
    18841 gagcgcacgc tgaccaatca tatgcagagc attatggaca agctagaagt gaactccacg
    18901 attgaagcga tccagaaagc ccgggagtta ggttatttga agtgatcggt ggcgggagaa
    18961 agatgacttt tggggtatac ggatcttgcg ctttgaatga ttgcttgatt tactcatgca
    19021 ttgcgctgaa ttggggagta aactgggggt agattaggta atggaggaga ttatgatgaa
    19081 atatgcaccg tatagtctgc tagccttagc aggtatatta gtttatctga cagtgaatcg
    19141 ctatattaca atgcacagca ttaatgtcgt tcccccgatt ggcgcaatct tgtgtgttat
    19201 tttctttttc gtctggcgca atcacttgaa acagcaaggg tattatgatg attaaataga
    19261 tgaagtctca gcaattatgt actgacccca agagttagag tgaataattc taattttttg
    19321 gggtcacatc attttaaggg attgttttag tcatattgtg gtgcggtcat acactagata
    19381 gggctacaag ctcatccctc gaatgtattc aatttcttct taagttgtct tatcttttga
    19441 aaagattata gaccaataaa aagtcaagtt ctacggcggg cagacggtgg aatttgactt
    19501 tttaaatgag ctaataaaaa tgagtcaatc actagggttt atacgagcgt gttcttttgg
    19561 taaaatagga attgacgata gggaagatga gatcatatag gggagttaat cgatgtcaaa
    19621 gaagaaattg ttattaattg atggatatag tattgcgttc cgggcattct atgcgatgca
    19681 tagtcagttg caccggatga agaataagaa tggactacat acgaatgcgc tctatgggtt
    19741 ccataatatg ttagaagcgg tgatggaaaa agaacaaccg acgcatgcat tagtggcctt
    19801 cgatgcgggg aagactactt ttcggcatga gttgtttgat gattataagg gcggtcgtga
    19861 gagtatgccg gctgagttat ccgaacagat accttatttg aaggaattaa ttgcagcgta
    19921 tggtctacag acgtatcaat tgccggatta tgaagcggat gatattatcg gaaccttaag
    19981 tactcaagca tcagatgaat tcgaagtcgt cattattacg ggggacaagg atttgacgca
    20041 actggccagt gatcagaccc gggttgatat tacgaagaag gggattacgc agttgaagtc
    20101 gtatacgccg gaatcaatga tggaagagat ggggattgcc ccagaacaaa ttaccgatct
    20161 gaagggatta tcgggggatt cttcggataa tatcccaggt gtgacacaga ttggagataa
    20221 gacggcgctt aagttgttga agaagttcca tacagtagaa gaattgtatg aacgtattga
    20281 tgagatgaat gcctctaagc gcaaggaata tttaatcgct gagaaagaaa cagcgctact
    20341 tagcaagcaa ttggccacta ttattactga tgcacctatt gagatttcac cggagtcatt
    20401 agcttattca ggaaaaaata tggaggcgtt gattggcttc tataaggaaa tggacttcaa
    20461 tagtcattta gaaaagttag atacggatgt gtacttcgaa gaattacaag atgaagcaga
    20521 agcggtcgaa tatgaatggg ttgaagaagt aacggaagca atgttcggac agagcgggac
    20581 gctgtatttt gaaatgttcg aagagaatta ccatacggca ccttatatta ttgcgagctg
    20641 gactaatgag aagaaagtct atgtagctga gccagagatg ttgttagctt ccaaggattt
    20701 ccaagattgg ctagaagatg agtcagcccg gaagtatacg tacgatgcga aagcattaat
    20761 ggtatccttg aagcaacgtg aattggagat tgctaatatt acttttgatt tatcgttggc
    20821 atcgtatgta ttgaccgctg agaattctag tggaggcgat gtggcggcta ttgccaacaa
    20881 acatggatat gtggatgtct tagaagatga agcggtctac ggtaaaggga aaaaagtaag
    20941 tattcctaat gataaaacag tcatgtatga gcatgtagcc cgtaaagtaa cagcaattga
    21001 tgccttagca gagcagttaa cagtggaatt ggcggataat gatcagttag ctctattcga
    21061 agagattgaa ttgccgcttg ctcatgtctt aggtgagatg gagattcaag gtattacgac
    21121 agatgcgaat cgtctggaga ctatgaagga agaattcgct gacgtattag aagagattga
    21181 ggctaagatt tatgcagaag caggcgaatc attcaatatt aattctccga aacaattgag
    21241 tgatgtactg ttcagtgaag ataagttgaa ctatgagccg attcgtaaga cgaagacggg
    21301 ttactctact gcgcaagatg tcctggaaaa gatgacgtcc tatgcgccca ttgctaagct
    21361 gattctggag taccgaacca ttgcgaaact ccagtcaacc tatgtggaag gcttgttgga
    21421 tgtaatcgaa gcagatggca aaatccatac gcgttatggt caaaccgtgg ctcggacagg
    21481 tcgcttaagt tcagttgatc cgaatctgca aaatattccg gtgcgttctg agatggggcg
    21541 gaagatccgc caagcgtttg ttccaagaga agatggatgg aagatcttcg gggcagatta
    21601 ttctcagatt gaattgcgta ttcttgccca tatgtcggaa gatgaagctt taattgagac
    21661 gtatctgaat gatcaagatg tccatacgac aactgcgatg aaagtcttcg atctagagtc
    21721 accggcagaa gtgacagcaa atcaacgtcg cgatgccaaa gcggttaact tcggaatcgt
    21781 ctacgggatt agtgattatg gtctatcgga gaacttaggt attacgcgaa aagaagcacg
    21841 agagtatatt gatacgtact ttagtcgctt cccgaatgtg aaggaatata tcgatcaatc
    21901 catccgtgaa gcgaaggaca aaggttacgt agaaactctg taccaccgtc gtcgttactt
    21961 gccagatatt aattcgagaa atttcaatgt gcggtcattt gcggagcgaa cagcgatgaa
    22021 tacgcctatt caaggcagtg cagctgatat tattaaagtg gcgatggttg agatgcaaaa
    22081 acgactgaaa gcatcagacc tgaaagcgac gatgctcttg caagttcatg atgaattaat
    22141 cttcgaagca ccggaatcag aattggctga attagaagcg ttagttagtg atgtcatgga
    22201 acatgtggtt gaactggatg tcccgctaca agttgctagc agttctggcg ataattggta
    22261 tgaagcgaag taagcataat agtaactgag atgatgaaga ggaggatata gatgccagaa
    22321 ttaccagaag tcgaagcggt gaagcgagga ttagatcaaa gtattgtggg gaagcgcgta
    22381 acagctattg aggtgatgtg gtctaagatc attcggcatg atgatataga ggcatttaaa
    22441 caatcactta ttggtcaagt atgtcagcga atagatcgtc gcgggaaatt tttgttgat
//
