LOCUS       NZ_CP040414            12154 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3090 chromosome, complete genome.
ACCESSION   NZ_CP040414
VERSION     NZ_CP040414.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 2009643)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009643)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 423x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655685.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:23:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,936
            CDSs (total)                      :: 1,864
            Genes (coding)                    :: 1,836
            CDSs (with protein)               :: 1,836
            Genes (RNA)                       :: 72
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 9 of 28
            Pseudo Genes (incomplete)         :: 15 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 2 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:01:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 149416
            Orig. end                         :: 161570
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040414.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    163..175
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.62"
                     /tool="antismash"
     gene            250..1353
                     /locus_tag="FE331_RS00920"
                     /old_locus_tag="FE331_00890"
     CDS             250..1353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724641.1"
                     /locus_tag="FE331_RS00920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00890"
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="WP_004635206.1"
                     /transl_table=11
                     /translation="MSKIIIMIWVFVLGSIGYNTLPEIMQFVTLPFKIPITFDWIGAIL
                     GGVIGYLAGLFTHKPIERSLNKVTHIFSKLQLSYLLFGSIGLVFGLIISWLISFSLQSF
                     NIPIISNYLPFVIAALLGYLGFYVGSSRHRELTAIFRSQQEHTNIVDKEVAEGFYKYKI
                     LDTSTIIDGRILDVVRTGFMEGTLVVSKHVLRELQHIADSADSLKRTRGRRGLDILNEL
                     QSDEAIQVEMNDQDLHETEEVDLKLLHLAKELHGVVITNDFNLNKVAQFQNVKVLNVNE
                     LASVMQPAVIPGEQMKVNIVKKGTEHDQGVAYLDDGTMVVVEGGQRHIGSSQHVVVTSS
                     LQTNSGRMIFAKLVTNQKSLDHKGYHD"
     misc_feature    255..268
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 24.12"
                     /tool="antismash"
     gene            complement(1425..1799)
                     /locus_tag="FE331_RS00925"
                     /old_locus_tag="FE331_00895"
     CDS             complement(1425..1799)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE331_RS00925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE331_00895"
                     /product="hypothetical protein"
                     /protein_id="WP_208957393.1"
                     /transl_table=11
                     /translation="MSKILSISTLFQSVEVHGNIPHESYEHRIVLLCVDEDISDPQLYQ
                     KIYDTLEEESYETADGVIVQWRLQKILDIFELVDALPVQSTDFPIEVYSRYFFDDGTLQ
                     DVLDKYFSDFVWEDNYLNNI"
     gene            complement(1850..2596)
                     /locus_tag="FE331_RS00930"
                     /old_locus_tag="FE331_00900"
     CDS             complement(1850..2596)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="FE331_RS00930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00900"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_208967822.1"
                     /transl_table=11
                     /translation="MYTVFVYDKVAENLNMLTTFVQHYINFKSNQLKFPLATQEAVTLL
                     SYLQTHNVINGIYLLSIGLNTDLCELISLSESIRRYDLTGKIIFLTPNRGQLISHTIEK
                     NIEFFAVIDILSPADTLQKNIHETIQKAENRLDMLLAQSNELIVIKSGYNLYRFDLKEV
                     LSIETNVECSHQLKVHSLHSSQYVSGSLKSIHEQYSKFLRVSRSHLINPNNVSCINFAE
                     YNIYMIKDHIVPMSRRYKNDIKNILV"
     gene            3413..3622
                     /locus_tag="FE331_RS00935"
                     /old_locus_tag="FE331_00905"
     CDS             3413..3622
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE331_RS00935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE331_00905"
                     /product="hypothetical protein"
                     /protein_id="WP_208953401.1"
                     /transl_table=11
                     /translation="MDIFILGIPAAQITKIKDYIAVLGGIKMAAKLLVGATTVNEKLQG
                     TLAALGSILGTLTGVTGVYEHCLR"
     misc_feature    4031..4044
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(4032..4045)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     misc_feature    complement(4057..4071)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 21.66"
                     /tool="antismash"
     misc_feature    complement(4076..4091)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.79"
                     /tool="antismash"
     misc_feature    complement(4281..4294)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.35"
                     /tool="antismash"
     gene            4338..4985
                     /locus_tag="FE331_RS00940"
                     /old_locus_tag="FE331_00910"
     CDS             4338..4985
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /locus_tag="FE331_RS00940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00910"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_208967823.1"
                     /sec_met_domain="Abi (E-value: 5.2e-15, bitscore: 52.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIATLIISVGQIPRDIFPAFVEDNMPYISRYIYTIVAFILLIWWL
                     KWWNKSVTPIYMNNTLFQKKNWVYGIGGYVLIFILVLLFILAKGYIFGSNSVEISKTLL
                     DEFAFEQNVYLGSLSILFFIFLGPILEEFIYRLGILGQLVEANTPTWLAVILSALIFSF
                     SHHNGIISQHFISGLGYSLLMIKTKSIYPVIIAHILFNTTTLIYRWYMLYYL"
     misc_feature    4361..4380
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            5001..7154
                     /locus_tag="FE331_RS00945"
                     /old_locus_tag="FE331_00915"
     CDS             5001..7154
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292468.1"
                     /locus_tag="FE331_RS00945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00915"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_112779165.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.3e-43, bitscore:
                     144.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 3.2e-239, bitscore:
                     792.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDTFKFVKQQDIMDCGVACIQMILKHYDSDMPAHKLRHMTGTDI
                     DGTSALGLKSALEELQFECLAVQADNSVWTDPDMNYPAIAHVLLEDNRQHYIIIYGFKN
                     NKLLIADPAEGKYKMSPEEFTTIWTNILLIPKPNKQYQPVVEKIGGLTSFLPALFKSKK
                     IMFFTIIASFLATSLSIVSSYYFQGIIDRVIPQEDLSLLNIMSIGLLGVYLLRVLFDYI
                     RSQLLIILGQSMSSHIMLDYFKHVLYLPMQFFNTRKNGDIISRFLDANKIIRALASSAL
                     ALFLDITMVAIVGTFLFIQNRILFVITLLSLPIYFVTVLFFVKRYNKANEEEMSSSAIL
                     NSSIIESLDGMETIKSYNSEQNVYETVQNQFLNLMKKSYKTVTLDNMQRSIKQSVQLLT
                     SAGVLWAGSYYILHNSMSIGQLITYNALLVFFTNPLENIINLQAELQTAEIASKRMNEV
                     LAIESEYNLTDKQAAITFDGGIRINHLTFSYNLKESTLKNITCHIPFNQTVALVGMSGS
                     GKSTLAKLLLRLHEVAEGTIQYDNILINKIPHNYLRDHVTYLPQESFFFKGTIIENLLF
                     GLSHQPSELEIITACERAKVRHVIDSLPLGFNTPLEEGAANLSGGQKQRLAIARALLRD
                     TNIYIFDEATSSVDTVTEQKIIQSISELKNKLIIHITHHLPIAKQCDQILVMHDGQLVE
                     EGTHEQLLANSGTYHTLWQSTFP"
     gene            complement(7211..7774)
                     /locus_tag="FE331_RS00950"
                     /old_locus_tag="FE331_00920"
     CDS             complement(7211..7774)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="FE331_RS00950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00920"
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="WP_208967824.1"
                     /transl_table=11
                     /translation="MIDINLGADMTGVDLAEAIRDSDIQSKIIFITSEEDQATNILNRH
                     IEPLAYISKNEGLETMQSNLHRALDDAYSRFSEITVRTKDVFSFNFDTLTYQFDLDEVI
                     SIEVSGDHRLTLKTITGQYDFFNTLAQIEQDYPTLLRIGRSDIINPVNIKHIDYKRRNV
                     TMINDEQFTIAASRIIQLRQLYKS"
     gene            complement(7771..7947)
                     /locus_tag="FE331_RS00955"
     CDS             complement(7771..7947)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE331_RS00955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_208967825.1"
                     /transl_table=11
                     /translation="MHSIIICEDNQEQLAYLSMLVQNYIQFHENQFRLVYEGTDPQTTL
                     NYIQQEAIKKDST"
     gene            complement(7948..9078)
                     /locus_tag="FE331_RS00960"
                     /old_locus_tag="FE331_00925"
     CDS             complement(7948..9078)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635172.1"
                     /locus_tag="FE331_RS00960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00925"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_244304880.1"
                     /transl_table=11
                     /translation="MVLYGIAIYRYFLNDRANRKRELIHILLFVLIKGSIEFLTALPIN
                     LLALGHLHSHVVVFLLASIVDVGLSVTVYKQVIKYFKNITLSPYETDILRHVLLGYNLA
                     LVFYFGYMKYQALYFDLLVFTSVILVGNGIVVGLVLFYVSRYSETRTREEAISKRLDYM
                     VKYTNIIEENQLELKRFKHDYQNLLLSLSILAESGDLDKLKQKTTSLRQYSAQQLLKEY
                     TQYSDLARVHHTLLKSILIAKLTELHHMNIVFRFVCPEPVESLNIADFDLIRMVGILID
                     NAREHVKDMEGGMIAVSVHKTTRGTHIKIENSYANQVTSIATLMTPGYSSKDNHSGLGL
                     SNVEKIKNKYDHVFLNYHIDDKFEVTLIIMNEEGEY"
     gene            9734..10537
                     /locus_tag="FE331_RS00965"
                     /old_locus_tag="FE331_00935"
     CDS             9734..10537
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635171.1"
                     /locus_tag="FE331_RS00965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE331_00935"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_112779162.1"
                     /transl_table=11
                     /translation="MNKQHPILELKNIRKSFDGVVVLDDITLAVYPGEIIGYVGSNGAG
                     KSTTIKIILGLLSADEGSVRVFGEEINTDDVLYKKRIGYLPENIALFDELSAKEYFEFL
                     ADLHGLNVRTAVDRSSKLLLLLNIDDEQFQSRIASYSKGMRQKVSIVASLLHNPDILFW
                     DEPLNGLDASSIQVIKAVLNELRDKGKSIFYSSHIMDTVEKLSDRIILLDNGHIKIDAA
                     FEDLKDSSDYNLEKLFNHVTGFDKREELAQKFIEILYEEGGAVNG"
     misc_feature    10627..10640
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    complement(11050..11065)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
     misc_feature    11061..11074
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.55"
                     /tool="antismash"
ORIGIN
        1 attgctttat cggagagatt ggcttaactg gcgaaattcg acgagtctct aaaattgaag
       61 cacgggttac ggaagcagct aagttaggct tcaagcgtgt ctttattccc aagaataata
      121 tagacggctg gacgcatcct gagggtatcg aagtcatcgg ctgtgccact gtctcagaag
      181 taatgaagcg aacatttaac taattgtttg cacgtaataa tttaacagaa aaataaaaag
      241 gaggatgtta tgagtaaaat tattattatg atctgggttt ttgtcctagg tagtatcggg
      301 tacaatacct taccagaaat tatgcaattc gtgacgctgc cgtttaaaat tccgattaca
      361 tttgattgga ttggagcgat tctcggcggt gtaattggct atttggcggg attattcacc
      421 cataaaccaa tcgaacgcag cttgaataaa gttacccata tattttcgaa actgcaatta
      481 tcttatttat tatttgggag cattgggctg gtgttcggac tgatcatttc atggttgatt
      541 agcttctcac tgcaatcatt caatattcct attattagca actacttgcc cttcgtgatc
      601 gccgccttgc tgggatactt gggcttctat gttggaagta gccgtcaccg ggaattgacc
      661 gccatcttcc gtagccagca agaacatacc aatatcgtgg acaaagaagt cgcagagggg
      721 ttctataagt acaagatatt agatacaagt acgattattg atggtcggat tcttgatgta
      781 gtccgtacgg gtttcatgga aggaacatta gtcgtcagta agcacgtctt acgtgaattg
      841 caacatattg ccgattctgc agatagcttg aagcgtacgc gcgggcgtcg cggcttggat
      901 atcttgaacg aactccagag tgatgaagcg atccaagtag agatgaacga ccaagacttg
      961 catgaaacag aagaagttga cttgaagcta cttcacttag cgaaggaatt acacggtgtc
     1021 gtcatcacca acgacttcaa cttgaacaaa gttgcccaat tccaaaatgt caaagtccta
     1081 aatgttaatg aattggccag cgttatgcaa ccagctgtca ttccaggcga acagatgaaa
     1141 gtcaatattg tcaaaaaagg aaccgaacat gatcaaggcg tggcctatct tgatgatggc
     1201 acgatggttg tcgttgaagg cggtcaacga cacatcggta gtagccaaca tgtcgttgtt
     1261 accagctccc tgcagaccaa ttctggccgc atgatcttcg ctaaattagt aaccaaccag
     1321 aaatcgttag atcacaaggg atatcatgac taaataaaaa aactaaaagt attgctcctg
     1381 agtagttcag gagcagcgct tttagtagtg tatatttaaa cattttaaat attgtttaaa
     1441 tagttgtctt cccagacgaa atcagaaaaa tatttatcta aaacatcttg caatgttccg
     1501 tcatcgaaga aataacgact gtaaacttca atgggaaaat cagtggattg caccggtaat
     1561 gcatcaacga gttcaaatat atctaaaatt ttctggagtc gccactgaac aatcactcca
     1621 tcagccgttt cataagattc ttcttccagt gtatcgtaaa ttttttgata tagttgggga
     1681 tcagagatat cttcatcaac gcaaagaagt acaatacgat gctcataact ctcatgggga
     1741 atgtttccat gaacttcgac actttgaaat aacgtactaa ttgataaaat cttgctcatt
     1801 atcttctcca tctagaatct tgtaatagtt ttttagcttt taatctattt taaactaaaa
     1861 tattttttat atcattttta tatcgacgcg acatagggac tatatgatct tttatcatgt
     1921 atatattata ttcagcaaaa ttaatacagg acacattgtt gggattgatg aggtgagaac
     1981 gactcactcg aagaaatttt gaatattgct catgaataga ttttagtgat cctgagacgt
     2041 attgtgaact atgaagtgaa tgaaccttta gttggtgaga acactccaca ttggtttcaa
     2101 tcgataatac ctctttcaaa tcaaatcggt aaagattata tccagatttg ataacgatta
     2161 attcatttga ttgtgccagt aacatatcca gacgattttc ggctttttgt atagtttcgt
     2221 gaatattctt ttgaagagtg tctgctggac ttaatatatc aataacggca aaaaactcaa
     2281 tattcttttc aatagtgtgg ctaatgagtt gtccacgatt tggtgttaaa aaaataattt
     2341 taccagttaa atcgtatcgt cggatcgatt cggacaaact tatgagttca cataaatcag
     2401 tgtttaatcc aatactaaga aggtatatac cattaatgac attgtgagtc tgcagataac
     2461 ttaataatgt cactgcctct tgagtggcta aggggaattt taactgatta gatttgaagt
     2521 taatatagtg ttgcacaaat gttgttagca tatttaaatt ttctgcaacc ttatcgtaga
     2581 caaaaacagt gtacatttta acgcctcctt ttttattatt atagcagatt tcaatgagtc
     2641 tgtgagtcgt ttttacttag aaaatatata caggacagga gaacagtaat agaaaataaa
     2701 ttatctagaa ttttttttat ttagaaaacc gtttaggcaa ataagttaat catttaggaa
     2761 gtttttgttt taagagtgga aatcatgcta ttatttggta acgcatttat tagataggta
     2821 ggcgatcaag atgtctagag aaaatttgtg aaataaaacg gtagtgggga aacaattgaa
     2881 gaattgcatc aacaagcatt agaataaaca gagaatatag ttaaaatatt tatggaatga
     2941 agatttgatt gtttcagtaa taaaaataat cagggagttg gttgcaaatt ctagagtaat
     3001 attagtgatt ttttagaata aagcaaaaaa agtgtttttg tgaaaggaat gacagggatg
     3061 aaaaaaatat attatatatt acattttttt ctttattatt tgtgcttgca gtggttagct
     3121 atatgtggcc tgaattttat gaactagaaa aaatcttaat gtttataata gctttgaatt
     3181 tattagcaat aatgctagtt cgggaacaaa aacacaatga ttagtgatat aacttcattt
     3241 tcgcacagat actatggctt ataattattt attttagaag aaacagaaga gacggttgaa
     3301 ttatttatgg aatggggatt tgataacttt aaaaataaaa ttaatcagaa acatcgtcaa
     3361 gagattatta gggagttagt tgccattacc gaagagaaga atgctttaaa taatggacat
     3421 atttatatta ggtattccag ccgcgcaaat aacaaaaata aaagattata ttgctgtttt
     3481 aggtggtatt aagatggctg ctaagttact agtaggagct actactgtta acgaaaaatt
     3541 acaagggact ttagctgctt taggatctat tttagggacg ctaactgggg tgactggtgt
     3601 ctatgaacat tgtcttcgat aagggatgtg ggagattgat ggatacggaa gctttaaata
     3661 ttatattagt acttatattg tttagtttaa tctatttaca aaggaagcag attcgaaatc
     3721 ttaaaaaaga aaattctatt ttgagagata aaataaaaag attaaggaag aacaaaaggt
     3781 tgtagatgaa ctgtccttat tgatataagg gtcgaaatac ttatgggaca atgcaagttg
     3841 taaaattatc gcgaaacttt taaaaaacag atttaaaaca gagtctattc gggtttaatg
     3901 agtaggctct tttagttgca caaaaatcaa tgttacacaa tatctgtcag atgcactatt
     3961 ctaaaaatag actgctctcc cgagctgtcc tagttccaaa aagctaccag tatctaggag
     4021 tagccactag attttttcat aaaatccgcc tttctcccac gcatgttgtg tgaattgttc
     4081 gcttatgggt ttggtggtgt gatcctgtca ttgaccgtta aaaggcgaca aatcagtccg
     4141 tgaacaagaa aatttaccag tatttccctg ttcacggact caaatccacc gattcacgaa
     4201 atattccagc gatttgttaa atttttggta tagtggatat attcataaca atcttattaa
     4261 ggagatacac atatgactag ttttatcaaa aaataggtag tccagttaaa attatatttt
     4321 taggtattat attttctgtg attgctactc tcattatttc ggttggacaa atcccccgag
     4381 atattttccc agcttttgtt gaagataata tgccatacat aagtcgttat atctatacaa
     4441 tagttgcatt tatactttta atttggtggc taaagtggtg gaataagtcg gtcactccga
     4501 tctatatgaa taatacactc tttcaaaaaa agaattgggt ttatggtata ggtggctatg
     4561 tcctaatttt catacttgtt cttttattca tactagcaaa aggttatatc tttggaagta
     4621 acagtgtcga aatatctaaa acactgcttg atgaatttgc atttgaacaa aatgtgtatc
     4681 tgggcagctt atcaatccta ttttttatct tccttgggcc tattttagag gaattcattt
     4741 atcgattggg tattcttggg caactagttg aagcgaatac tcccacatgg cttgccgtta
     4801 ttttatctgc attgatattc tcgttcagcc atcataatgg cattatctca caacacttta
     4861 tctcaggact tggctactcc ctacttatga taaaaacaaa gtcaatctac ccagtcataa
     4921 tcgctcatat cttatttaat acaaccacac ttatttatcg ttggtatatg ctatattatt
     4981 tataaaggag taatattatt atgcgagata cttttaaatt tgttaaacag caagatatta
     5041 tggattgtgg tgtggcttgt attcagatga ttttaaagca ctatgattct gatatgcctg
     5101 cccataaact ccggcacatg acaggtactg atattgacgg aacttctgcc ctaggattga
     5161 agtctgcatt agaagaacta caatttgaat gtctagctgt tcaagcagat aatagtgtgt
     5221 ggacagatcc tgatatgaac tatcctgcta ttgcccatgt cttactagaa gacaatcgcc
     5281 aacattacat tataatttat ggattcaaga ataataaact tttaatagct gacccagctg
     5341 aaggaaaata taagatgagt cccgaagaat ttactactat ttggacaaat attctcctta
     5401 ttcctaaacc taacaaacag tatcagcctg ttgtcgagaa gattggtgga ttaacctcgt
     5461 ttttaccggc tttatttaag tctaaaaaga taatgttttt tactataata gcttcatttt
     5521 tagctacatc actgagtatt gttagttcat attattttca aggaattatt gatagagtta
     5581 tccctcaaga ggatctatct ctactcaaca ttatgtctat cggattatta ggtgtgtatt
     5641 tacttcgagt attgtttgac tatattagaa gtcagttgtt aattatttta ggtcagagca
     5701 tgagtagtca tattatgcta gactatttta agcatgtcct gtatcttcct atgcagtttt
     5761 ttaacacacg aaaaaacgga gacattatct cacgattttt agatgcgaat aagattattc
     5821 gagcacttgc tagttcagct cttgctctat tcctggatat aacgatggtt gctattgtag
     5881 gaacattttt atttattcag aatagaatct tatttgtaat tacacttcta tcacttccta
     5941 tatactttgt cactgtatta ttttttgtga agcgttataa taaggcgaac gaagaagaaa
     6001 tgagttcctc agcaatacta aattcaagta ttattgaaag cttggatggc atggagacaa
     6061 ttaagtcata taatagcgaa caaaatgtat atgaaactgt tcaaaatcaa ttcttaaatc
     6121 taatgaaaaa atcctataaa accgttacat tagataatat gcaacgttca attaaacaat
     6181 ctgtccagtt actcactagt gcaggggttt tatgggcagg gtcatattat attcttcata
     6241 attcaatgag tattggtcaa ttgattacct ataatgcatt attagtattt ttcactaacc
     6301 cactagaaaa tattattaac ttacaagcag aactacagac cgctgaaata gcaagtaaac
     6361 gtatgaatga agttcttgcc attgaatcag agtacaatct cactgataaa caggccgcta
     6421 tcacttttga tggagggata cgaataaatc atttgacatt ttcttacaac ttaaaagagt
     6481 ctactctaaa aaatattaca tgtcatattc cattcaatca gacggttgct ctagtcggaa
     6541 tgagtggctc tggaaaatct actctagcca aattgttgct acgacttcat gaggtagctg
     6601 aagggactat tcagtatgat aatatactga ttaataaaat tccacacaac tatttacgag
     6661 accatgtgac ataccttcca caagaatctt ttttcttcaa aggaacaatt attgaaaatt
     6721 tattgtttgg gttatctcac caacctagtg aattggaaat tataactgct tgtgaacgag
     6781 caaaggttcg acatgtgatt gacagcttgc cattaggatt taacacacct ctagaagaag
     6841 gagcagctaa tctatctggt ggtcaaaaac aacgtctagc tattgctcga gcgttactta
     6901 gagatacgaa tatttatatt tttgatgaag cgacaagtag tgtagataca gtcacagaac
     6961 aaaaaattat tcaatcgatt tctgaactaa aaaataaatt aattattcac ataactcacc
     7021 acttgcctat tgctaaacaa tgtgaccaaa tacttgtaat gcatgatggt caacttgtag
     7081 aagaagggac tcatgagcaa ttgttagcca attcaggaac gtaccacaca ttatggcaat
     7141 caactttccc ataaactcaa aaaaagatat gatgctatct attggttgtc catgcatcat
     7201 atctttttta ttatgattta tacagttgtc gcaattgtat tatccggctg gcagcgattg
     7261 tgaactgttc atcatttatc atcgtcacat ttcgccgctt ataatcgatg tgcttgatgt
     7321 tcacggggtt gattatatca gaccgtccaa tacgaagcag tgtgggataa tcttgttcga
     7381 tctgcgccaa tgtgttaaaa aaatcatact gtcctgtgat cgtttttagc gtcagtcggt
     7441 gatcgccaga aacttcaata ctgatcactt catccaaatc gaattggtag gttaatgtat
     7501 caaaattaaa tgaaaataca tctttggttc gaaccgttat ttcactgaag cgactgtatg
     7561 catcatctag ggcacgatgt agattagatt gcatagtctc taatccttca ttcttcgaaa
     7621 tataggctaa tggttcaata tgacggttca gtatattagt tgcttggtct tcctcactag
     7681 taatgaagat aattttggac tgaatatcac tatcacgaat agcttctgcc agatctaccc
     7741 cagtcatgtc agcccctaag ttaatatcaa tcaagtagag tcctttttga ttgcttcttg
     7801 ttggatatag tttaaggtag tttgcggatc ggtgccttca tagactagcc tgaattgatt
     7861 ctcatgaaac tggatgtaat tctgaacgag catcgataga taggctagtt gctcttggtt
     7921 atcttcacaa ataataatag aatgcatcta gtattctcct tcctcattca ttataatcag
     7981 agtgacttcg aatttgtcat caatgtgata attaaggaat acatgatcat atttgttttt
     8041 tattttttca acattcgata gtcctagtcc cgagtgatta tctttagatg aatatccggg
     8101 cgtcatgagt gtcgcaattg aggtcacttg gttggcatat gaattttcga tttttatatg
     8161 ggttccacga gtcgtcttgt ggacagatac agcgatcata ccaccctcca tatccttcac
     8221 atgctcgcgc gcattatcga tcagtatgcc taccatccgg attaaatcga agtcagcgat
     8281 attcaacgat tcgaccggtt caggacagac aaatcggaac acaatattca tatgatgaag
     8341 ttccgttaac ttcgcaatca agatactctt caagagggtg tggtgtacgc gcgctagatc
     8401 actatattgc gtatactcct tcaataattg ttgggcagag tattgtctca aggaagttgt
     8461 tttttgtttg agtttatcta agtcccctga ttctgctaag atagataagc tcagcaacag
     8521 attctgataa tcatgcttga accgcttaag ttctagttga ttctcttcaa taatattcgt
     8581 atacttcacc atataatcta accgcttact aattgcttct tctcgtgttc tcgtttcgga
     8641 ataacgcgat acatagaaca agaccaagcc tacgacaatc ccattcccca ctagaataac
     8701 acttgtgaat actaacagat caaaatataa cgcttggtac ttcatatacc caaagtaaaa
     8761 aactagtgct aaattgtatc ctaatagtac gtgtctaagg atgtctgttt catagggtga
     8821 taaggtaata tttttaaaat atttaattac ttgcttgtat acagttactg agagaccaac
     8881 atcaactatc gatgctaata gaaatacgac gacatgacta tgcagatggc ctaacgccaa
     8941 taaattaatt ggtagagcag ttaaaaattc aatacttcct tttataagta caaacaatag
     9001 tatatgaata agctctctct tacgattcgc acgatcattt aagaaataac gataaatggc
     9061 tattccatat agaaccattg agaggttacc tatgcgtaaa taaccttcta aaaagagtga
     9121 taaaagccaa aatagagtgc ccaaaagaaa taagcttata atagattttt tctcaatatt
     9181 aaatagataa atgaaagcta ttgtcataaa aatataatgc aagcctattt ctaatataac
     9241 agctaccact ggattaatta aaatcacaat caccctttcg ctatttttaa caatttttcc
     9301 ttaggttgaa caagcatctt agtccaaccc acgagtcact attcctatga tgctgtccac
     9361 ctaaaatatt tttcaactgt ttggttgtta attttttcat cacaattcct cctctctatt
     9421 attcaataaa atcatatcat agtcaatcta ttcattcacc aaaactccgc gaattggcta
     9481 atatcattcc ctgaacaagg gaccaacatt taattttact gtttaaggac tcaaatccac
     9541 caattcacga aaaataaaaa gattataatt tttttattgt tatactagta atgtggaaga
     9601 tacgtcagat gagtaatata acaccaaata atgtaaaata cttttttgat aaaaaaatgt
     9661 atatgctata ctgggtaagt atttaaatta taaacgaagt tggtcaagta ttaaattaag
     9721 aagggaataa ttgatgaata aacaacaccc tattctagag ttgaaaaata tacgtaaatc
     9781 atttgatgga gttgtcgtat tagatgacat tacgttagct gtctatcccg gtgaaattat
     9841 tggttatgtt ggatcaaatg gagccggaaa aagtactact ataaaaatta ttttgggctt
     9901 actatcggct gatgaaggat cagtgcgtgt atttggtgaa gaaataaata cagatgatgt
     9961 gctctataaa aagcgcattg gctatctccc agagaatatt gctttatttg atgaattatc
    10021 tgcaaaagaa tattttgaat ttttggcaga tttacatggg cttaacgtac ggacagcagt
    10081 agatcgcagt agcaaattat tattactatt gaatattgat gacgaacaat ttcaaagtcg
    10141 aatcgcatca tattctaaag gaatgcgaca aaaagtcagt attgtcgcta gtttattaca
    10201 taatcctgat atcttatttt gggatgaacc gcttaatggt ttagatgcaa gtagcattca
    10261 agttattaaa gctgtattaa acgagttgag agataaagga aaatctattt tttattcttc
    10321 tcatattatg gacacagttg aaaagttaag tgaccgaatt attttattag ataatggcca
    10381 tataaaaata gatgctgcat ttgaagatct taaagatagc tcagactata atttggaaaa
    10441 actatttaat cacgtaactg gatttgataa acgtgaggaa ctagcacaga aatttataga
    10501 gatcttgtac gaagaaggtg gggcagttaa tgggtaaagg gaaattttta aatcatgcgg
    10561 atagacgtta tcccgcctat atggtgttgg taaaatggct atttcctgtt tacagaatga
    10621 tgggcgttaa tcctgatcaa gtctttgcaa tcgtacattt gaatatgata ttagatgagc
    10681 gaaaagacaa gatgcctggt atgcgagaca aaaataacaa ccagctaatt tggttgattt
    10741 taatttttgc tatattagga atcatattac tatatgattt atttcgcttt gatacagtat
    10801 ttcagcagtt tagtatattt tttacaatgt ttatggggct tatgtttttt attttaatta
    10861 catatttttc cgatgtattg ttaaaacaac ctgagcaaac tttaattaat gcacaaccag
    10921 taacaaacca aactgccatt gtagcgaaat ttacgcacat agggctctat gtgctattac
    10981 taagtatttc cctaggggca cctagtatca ttcgtgtagg atatatttat tcctccttaa
    11041 tagcactttt gtttgctctt ggcattatta ttgcaggtat ttgtgtatta ttaataataa
    11101 atttagtgtt tttaataata ataaatacag taggcgagca atatttacaa aaagtattac
    11161 tatctagtca agtagtagtg gctggagttg ccattttatc aggaacaatt cttagagagt
    11221 caattgagta ttttaccgac atagggaatt cattcgagat aaatttagtt tggtggcaat
    11281 ttttaatatt tccgatgtgg tttgctgcgc catttcaagg aataattaat ggcttcacag
    11341 tggagagcac actatttaca ttgttactaa taattagttt agtagtgggt tttgtactct
    11401 atagcaaaaa agcctatgtt attcaacaaa atttgttatt aaaaaatagt cgacaaaata
    11461 cgactgttca ccagtcatta ttaatgaaac taggtagaat attaagtaga gcgagtagca
    11521 tagaacgtcc atattttaat ctatattgga aattactcag tcgtgataca gttttaaaaa
    11581 caaaaatata cccaaattta gttcaggggt tgattgttcc gattgccgta ttggttccag
    11641 tatatatttc tagtgaacga agagcattta tactagaaaa tacccccagt agcttatttt
    11701 tcatacttta tacgactaca tttgctttac cggccgttat tcatttgatt caattctcaa
    11761 caagctataa agcattttgg ctgaaagaat ttgtgcctaa taatcagaat tctgttcagt
    11821 acagagcagc ttataaagtt atactgacta aactattact ccccatgtat acaatagcct
    11881 caatgttata tatactagct ttaggtacga tgcctattat tattttaatg aatggattct
    11941 tgtacaatgg tatggtcttg tattttttaa tggatgtctt tctaaaaact agtccattct
    12001 ctaaaaaaat aagatcttcg ctgatgggta taggttgttt tggacagtta gctggtatga
    12061 tcattatttc attaggaata atattgatta tgtttatagc aaataatgtg gtttatggaa
    12121 atatagcatt gtttactgtt cagagtatat ttat
//
