LOCUS       NZ_CP040411            22745 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3256 chromosome.
ACCESSION   NZ_CP040411
VERSION     NZ_CP040411.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1941401)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1941401)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 530x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655645.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:25:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,851
            CDSs (total)                      :: 1,781
            Genes (coding)                    :: 1,758
            CDSs (with protein)               :: 1,758
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 9 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:41:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1650759
            Orig. end                         :: 1673504
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..22745
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="4"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..22745
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22745
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12745](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12745
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            133..765
                     /locus_tag="FE334_RS07745"
                     /old_locus_tag="FE334_07720"
     CDS             133..765
                     /GO_function="GO:0004623 - phospholipase A2 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0050482 - arachidonic acid secretion
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636157.1"
                     /locus_tag="FE334_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07720"
                     /product="phospholipase A2 family protein"
                     /protein_id="WP_208965669.1"
                     /transl_table=11
                     /translation="MRKKCKKVVILSLVALISLVTVFSNNYIQVYAHDTGVVENNEKGS
                     SELKKSLETVEQYIDMDGDKKFDESLAREDNASKDTIETGRIYNEMVRLENQGEHDALV
                     EQRRSWISEATKYGNWCGKGDKGAPPIDLLDRQCQKHDKCYAANGWGNTQCDINFVHNI
                     GRNFDSIKNISAYAKNYAKAAVLLFAGKVGGTQALAEKFPALAPYLP"
     gene            822..1103
                     /locus_tag="FE334_RS07750"
                     /old_locus_tag="FE334_07725"
     CDS             822..1103
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_07725"
                     /product="hypothetical protein"
                     /protein_id="WP_111948892.1"
                     /transl_table=11
                     /translation="MKEKWIKKWQNLSFLSKIAHLSVIMLALFAPFVSLVVEGLFNIYI
                     VEPNHIFKFFYLTLVLCVIAREWKLLIGTFITFYVVFILLVWYGFVFG"
     gene            complement(1564..2085)
                     /locus_tag="FE334_RS07755"
                     /old_locus_tag="FE334_07730"
     CDS             complement(1564..2085)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607643.1"
                     /locus_tag="FE334_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07730"
                     /product="isochorismatase family protein"
                     /protein_id="WP_208965670.1"
                     /transl_table=11
                     /translation="MEALIILDVQEGMLKHGDYSQMLDTIQYLVDDFSREDKPIISFRH
                     IDDNEESSIYKGSTGAELVSVAKDHSDYIIEKRTPDIFSGTELQQILEEHNIDEVAICG
                     FNAEFCCLFSAIILTHNGIKVNYIEDATGSVNDGDVYEMADLDIVDFVGCVLDWSGVVD
                     VFYYEEYREK"
     gene            complement(2147..4912)
                     /locus_tag="FE334_RS07760"
                     /old_locus_tag="FE334_07735"
     CDS             complement(2147..4912)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008134 - transcription factor binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635086.1"
                     /locus_tag="FE334_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07735"
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="WP_208965671.1"
                     /transl_table=11
                     /translation="MERKEEIIHLLSNQPEKPFTTREVAEALGIQRSNASSYLNELTGE
                     GRLTKLGGRPVKYTLNEPSSSSPIPADTAPPRSKMTSDNQRDVFSQVIGHNGSVKNAVM
                     QGKASILYPPNGLNTLITGPTGSGKSFFANTMFQFAKNQGLIAADKELQVFNCADYAKN
                     PELLMSHLFGYTAGAFTGATTEKDGLIQQADGGMLFLDEVHRLPPEGQEMIFYFMDTGQ
                     YSRLGEADRKLHASVRIVCATTEDPKSTLLETFIRRIPINIQLPTFKQRPVSEQIDLLK
                     LMVGLEAKRINRKVSMSKDVVRTLISHVDYGNIGQLKSNIQLLCARGFMNQLHNNVIEL
                     TTHELPENLTRSFIAMTSDRSASSELIRRLEPTLEIHPEDVDIILDRDSYELPYNIYDI
                     IADKATLLEEEQIDQETINQYISTDINLHLKSFYKNQGFALQTTSKLAEVVSLEIIDFA
                     RDVLAMTDLETAPGQSGSLLYALSLHISSLIGKLRDGQLRPFNARVRDLALNTPDALRQ
                     AEVIRHRIADHFNLAVPESEAYYLTVLLTSFKTDITQQVGIVLAAHGPSTASSMAKVVA
                     ELLETPPIPAIDMPLSMSPTEAYTKIKEAVKHADDGSGVLLLVDMGSLATFGTKLELET
                     GVKVRSLDMVSTGIVLEAVRKASMTTTTLDELHQSLKHFTGYHQINAEPTTSPNHLKES
                     AILAICASGEGTAKKIKEIVEKPYANKADMPYQVIVMSAVNLQEHLPSVLSRYNIIAST
                     GIIDPKIAAPFIPLETFINMGASQLNQLLLGTHDPTEITDQTQAQQLVQRYIEDNHLYL
                     NPARITPILWDFAERLQECQKQEKGFAFHVNLSLHLAGAVERILTNSTIESASELPTDL
                     TEDMKQYIDRHIIQLERQLLLTLPDEEKEYIYYYVSKDAGEIDELDDLLD"
     gene            complement(5069..6349)
                     /gene="rpoN"
                     /locus_tag="FE334_RS07765"
                     /old_locus_tag="FE334_07740"
     CDS             complement(5069..6349)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpoN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635085.1"
                     /locus_tag="FE334_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07740"
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="WP_208965672.1"
                     /transl_table=11
                     /translation="MKITQQAMIKQTQAPLPFLIKSVGILQLNRVDLSHYISNYMSENP
                     LMTFANDDALMFGSESSAEGINLDEINASTQTLFDFITEQVELFYRQTPLRDMTMWWIE
                     QLDTNGYVTKTLEEAVQITGESDYMVLDGLTLLQQLDPPGIGARDLRENLMLQTERLDS
                     APNLAYIILEENFDRLVNRQWSDLATIYRTDLADIRAIFKFIQTLSPIPANLYQAPQTE
                     MIYPELEVTVANQQLTISETRFQTPLLTFDQSYYDELAQIDKPSIQDYIKAKKQEFDQL
                     QQALQKRKETILRVGTAILSYQKDYFLTEDASLQPLQINDLVKQLQLHESTISRAIRGT
                     YVQTNRGVIELKSLLSKRSVVKDTSQDAIFTALSDLIEGEDKTHPLSDQQLADTLKQTK
                     HMALSRRTIAKYRSQLGIPSTRERKIR"
     gene            complement(6351..7355)
                     /locus_tag="FE334_RS07770"
                     /old_locus_tag="FE334_07745"
     CDS             complement(6351..7355)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /locus_tag="FE334_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07745"
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_208965673.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 2.3e-29, bitscore:
                     99.3, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 6.4e-19,
                     bitscore: 64.9, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKLKWGIIGTGGIAEDFAAGFQDTGITCHGVSSRSLEKAEAFKE
                     AFGLHAAYGDYQELLQDEAIDIVYIATPHHTHAQIAADALQAGKHTVVEKPIVTQADDL
                     AQLKQLAEAQGVYLFEAMTIHYRPVYQTIRELIDQKELGALKMIQVNFGSFKPKDTGYF
                     FQKDLAGGALLDIGVYALNFVMEFLSTPPTEMKTLGYINEQFGVDESAGIVLKNDQNEL
                     ATITMTFHAKLPKRGVIAFEGGYFEIDNYPRADRITFTSPEGKIEHYQAGQSSQALQEE
                     MIAITNLIASGTPQPHLTKTTHVIELMDAIRQEWNLTYPFDNHTLDAPNREKA"
     gene            complement(7502..8455)
                     /gene="manA"
                     /locus_tag="FE334_RS07775"
                     /old_locus_tag="FE334_07750"
     CDS             complement(7502..8455)
                     /EC_number="5.3.1.8"
                     /GO_function="GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="manA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635083.1"
                     /locus_tag="FE334_RS07775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07750"
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="WP_208965674.1"
                     /transl_table=11
                     /translation="MSTEPIFLTPVLQDKIWGGKKLQTEFGFNLPSDTVGEAWVISTHP
                     HGESTVSSPEQYAGMGLNELYHQHPELFGVDEPGTFPLLTKILDAKEDLSVQVHPDDEY
                     GQEHEGELGKTECWFVISADEDATIIYGHNARTEEEFRRLVEAGEWDELLREVPVKAGD
                     FFYVPHGTIHAIKGGITILETQQNSDTTYRVYDYNRTDKDGNTRELHLEDSIAVSNIPH
                     IDPAIQQQEDRVGMSAITHYLTNEYFSVYRWQIRDQFVVDLTGDYTLVTVLDGQGMIEI
                     DRESYPVEKAQSFIIPHGVSSVTLSGDLDMIASNPE"
     gene            complement(8551..8880)
                     /locus_tag="FE334_RS07780"
                     /old_locus_tag="FE334_07755"
     CDS             complement(8551..8880)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_07755"
                     /product="hypothetical protein"
                     /protein_id="WP_111948904.1"
                     /transl_table=11
                     /translation="MIEDRDFLMRQIKQMIQNLGKILDRNTLRELLALSEDDMSNEELD
                     SLYLMGQVDIIAAEKQLTPQHLSVDLGIDIDRLEDLFKGQAFLTEPEATNVQSFLETQD
                     NSEPT"
     gene            complement(8905..9603)
                     /locus_tag="FE334_RS07785"
                     /old_locus_tag="FE334_07760"
     CDS             complement(8905..9603)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="FE334_RS07785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07760"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_208965675.1"
                     /transl_table=11
                     /translation="MNVFAFCGSLSDSSICEFAIHRYVDHLNAHSDVEINLFYTNGNSL
                     TINYNQGNNQEFYHGHSTDATDDMKKLESQLIKSDIIIFASPTYMHNVSGFMKNFIDRI
                     GYWTHLLKLRGKVGISISSCHSNGVNFTSEYLDKILAQLGCSIESSVKLSTSIQNKTVI
                     DSIIQYEAKKVMRKIRSKQFNITDFHEKKFETYKNMYHSQDTSESRYWQENNLFDFNNY
                     YDLFMKSCDL"
     gene            9724..9906
                     /locus_tag="FE334_RS07790"
                     /old_locus_tag="FE334_07765"
     CDS             9724..9906
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS07790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_07765"
                     /product="hypothetical protein"
                     /protein_id="WP_208965676.1"
                     /transl_table=11
                     /translation="MDFEMEKSFDESQLKKLAKNTPLVKESEIIVEVSAIACSITVSIL
                     LSPTSALSVTVTAGD"
     gene            10001..12745
                     /gene="lanM"
                     /locus_tag="FE334_RS07795"
                     /old_locus_tag="FE334_07770"
     CDS             10001..12745
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="FE334_RS07795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07770"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_208965677.1"
                     /sec_met_domain="DUF4135 (E-value: 1.4e-55, bitscore:
                     185.7, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.4e-39, bitscore:
                     132.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKEIKSKFTNDILAIIMRKVDDSISPAHLEYFSNKLIESISRDI
                     TNSFQDQIFDVIIVEFHKFREHNNFKDVDTTIDEFSSKIDRESFVEKLFFEYPLLEEKI
                     LSRIKGDTSFINKMLWDVRRDQVELEEAFNIDIETLCNVYINMGDKHDNKSVTLLKFKN
                     KNILYKPREIVFESTVETLIDSITESKCLKQEYIRRVKQPDRIIGKGYYWEEYIHEEKV
                     KEEDIDSFYYTSGVYLALFYILNITDIHFENILLKSDYPIFVDLEVGNIKFSKYEIKNN
                     HLLMNNSVLMTGLLPIYDKKLQLNMSGLFSHPQETNELSRQVPKYDKKLGIVYKKEAGK
                     IIPKNANLSHNNKKIAINYLLKGMSDCLHYIRTHKRDIVKQIDEYDDFKYRVLLRHTSV
                     YFSFIEALNTPKALRDKKQREKILNILEDNYIPNDGVGYVTLFEEIAQLKEGNIPIFKV
                     PFKQRGLYVGDEEILNEYVDTPLKEVVISRISCLDNFCIDYQLRLIKLSIASTVPVTQL
                     NTLNNVEHLSHQKVADDELKQNIGNLLNNIGSFSFELENGGVERYDLQYGENAFLEIAP
                     QKFNLYDTLANIALFYVFGNYFNEGKYEKIALSMYKNISEKVKYIMESDSKTNDEEDIS
                     VFEGISGVMYVSYYLYTITQSERYLEDTCEIYKFVIRKVKEKISHKKMNLDYISGLSGI
                     GLLLINILKHNSKVVKESDLMMIKGEIEKNYHTIEKNGLAHGKLGPLLFLIEYNLCVSR
                     TEDEIKNIGDRLLALSLDTENNTWCNGLSGYLYVLNEYKILLNDNKFIVQTINSKIKTI
                     IDNNILKNKSLSLCHGVFGNIEVLLKINENINIDRELNKKINLSYIKDLTKIKWCDLVD
                     VTASGFMTGATGIAYELMRIRNKKIPSIMFLEFFDDKGVKNYE"
     gene            12738..13586
                     /locus_tag="FE334_RS07800"
                     /old_locus_tag="FE334_07775"
     CDS             12738..13586
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="FE334_RS07800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07775"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_208965678.1"
                     /transl_table=11
                     /translation="MSNPAVSLEKVKKQFKNKYALKDINMSVEKGEIFGLLGPSGAGKT
                     TTIKIITKKETHDYGRVTIFGKKINDVGQDLLSRFGIVSDDSGYYKRLDLITNLKIHAK
                     LYDRSDSYVENLLKKMGLFEDRKTKAKNLSVGMRQRMFLARALIKEPVILFLDEPTSGL
                     DPQTSNVIHQLILDLKQNGTTVFLTTHDMKEAEKLCDTIGLLHEGKIIEKDTPQRIINK
                     FATESDNDMKVIFKNGDVKFMNTNSSEFESINNDDVMDMYRIELTLEDVFLKLTGDELD
                     V"
     gene            13579..14286
                     /locus_tag="FE334_RS07805"
                     /old_locus_tag="FE334_07780"
     CDS             13579..14286
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS07805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_07780"
                     /product="ABC transporter permease"
                     /protein_id="WP_208965679.1"
                     /transl_table=11
                     /translation="MFKRFKNILWLRIKQYISNPMYIILIALPYLFLWLYDLLIGDMVG
                     ADDLLFTVLPLLYTLTMGQLISSIVSEEKEYNNLMELQYSGVKGPEYILSTILYPLLLG
                     ILGIALLPMVIGGISEIDISYLSYYTINFLTAMCFALIYISIGMFSETVNQSQIIAIPI
                     MIVTLFLPMGAAYNNTVYEFTRFSFMGSFVELYNDSGNIFGSTSFISLLIWIVGLFVLA
                     LVSFIYGFRRRNW"
     gene            complement(14505..15029)
                     /locus_tag="FE334_RS07810"
                     /old_locus_tag="FE334_07785"
     CDS             complement(14505..15029)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052001.1"
                     /locus_tag="FE334_RS07810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07785"
                     /product="Gx transporter family protein"
                     /protein_id="WP_208965680.1"
                     /transl_table=11
                     /translation="MTKTQRMIYISLLSAQAVILGLVENSIPFPFAFAPGAKLGVANLI
                     VIIAIYTLPLKDSFKVLMMRLLMTTLLGGTLSTFMYSFMGALLSYLGMLLVKTLGRSQV
                     SIIGVSATGGMLHNLGQLLVASFIAQTWTVLMYLPVLSFFGILSGMAIGIAANYLMEHV
                     HTVRRFRASQD"
     gene            15175..15777
                     /locus_tag="FE334_RS07815"
                     /old_locus_tag="FE334_07790"
     CDS             15175..15777
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635079.1"
                     /locus_tag="FE334_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07790"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_208965681.1"
                     /transl_table=11
                     /translation="MSDHYYTKEPTSTSNPEQFKEVVIGQTLNLTSDAGVFSRGQMDFG
                     TRTLIEAVQTDKKCSGPLLDMGCGYGVIGIALAAANPERTIHMVDINERAVALAKQNAE
                     VNAVENVIIQQSSLFENIVERSFSAVVSNPPIRAGKQVVHGILEQSYDYLQPGGKLYIV
                     IQKKQGAPSAKKKMQAVFGNVERIALEKGYWVLMAEK"
     tRNA            15887..15959
                     /anticodon=(pos:1666679..1666681,aa:Val,seq:gac)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="FE334_RS07820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="FE334_07795"
                     /product="tRNA-Val"
     gene            15887..15959
                     /locus_tag="FE334_RS07820"
                     /old_locus_tag="FE334_07795"
     gene            16055..16432
                     /locus_tag="FE334_RS07825"
                     /old_locus_tag="FE334_07800"
     CDS             16055..16432
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898676.1"
                     /locus_tag="FE334_RS07825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07800"
                     /product="TIGR02328 family protein"
                     /protein_id="WP_208955243.1"
                     /transl_table=11
                     /translation="MRLWHETLISDLPRQQLLGQHRECCALRGKGWNRPHATVQYVFDY
                     SPYKLYQYHQLIMEEMKSRTYQPDERWEDPLYRGKACDPYRELEPVTPTKPIYPEHNAT
                     YLAECLENLAEKGIELSVSES"
     gene            complement(16499..17767)
                     /gene="tyrS"
                     /locus_tag="FE334_RS07830"
                     /old_locus_tag="FE334_07805"
     CDS             complement(16499..17767)
                     /EC_number="6.1.1.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tyrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635076.1"
                     /locus_tag="FE334_RS07830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07805"
                     /product="tyrosine--tRNA ligase"
                     /protein_id="WP_208965682.1"
                     /transl_table=11
                     /translation="MNIIDELQWRGAINQQTDEDGLKELTEKQSIALYCGVDPTGDSMH
                     IGHLIPFIILKRFQMAGHKPVVLIGGGTGSIGDPSGRNSERQLQTKETVQHNVDKLSAQ
                     MRKLFKAGEEESGIRLTNNADWLSPMSFLDFLRDYGKEFNINTMLAKDVVASRLEQGIS
                     FAEFSYQIIQSIDFLHLYRHEDVQLQIGGSDQWGNITAGLDLIRKKEGHEAKAYGLTIP
                     LLLKSDGTKFGKTAGGAIWLDPEKTSPYEFYQFWINQQDADVVKYLKYFTFLDQTEIAK
                     LEQSLTDEPWKRQAHKALAEEMTRFVHGQAALDEAIQITEALFSGEIADLTAEQIEETF
                     GDVPTSEATRGEHDIVEFLADITGICSSRREAREFIEQGAITISGEKVTDLDYTISSTD
                     TFDDRFIIVRRGKKKYYRVNLTD"
     gene            complement(18318..19292)
                     /locus_tag="FE334_RS07835"
                     /old_locus_tag="FE334_07810"
     CDS             complement(18318..19292)
                     /EC_number="1.1.1.27"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724307.1"
                     /locus_tag="FE334_RS07835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07810"
                     /product="L-lactate dehydrogenase"
                     /protein_id="WP_004635074.1"
                     /transl_table=11
                     /translation="MTQSEKKHKKIILIGNGAVGSAYAYALVNQNIGQELGIIDLDPFK
                     VEGDVMDLNSALAFTAPKDIYVADYSDCADADLVVFTAGAGQKPGETRLDLIHKNLKIT
                     KTIVDQVMASGFNGIFLVASNPVDILSYAVHKFSGMPAHKVVGSGTSLDSARFRIELAK
                     RLNIDARNVHGYIIGEHGDTEFPVWSHANIAGLQITEWLETNPLEDEGELLEIFEAVRD
                     QAYHIIERKGATYYGIGAALAKITQAIFNNENSVLPLSVLLEGEYGHDDIYIGTPAIIN
                     DRGVKRAIEIPLNDYEQGKMNHSINTLRKFIQDAREKAPELGL"
     gene            19610..20071
                     /locus_tag="FE334_RS07840"
                     /old_locus_tag="FE334_07815"
     CDS             19610..20071
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635072.1"
                     /locus_tag="FE334_RS07840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07815"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_111948914.1"
                     /transl_table=11
                     /translation="MDHQTMNEVNEYLVMIFNEVLSIEEEAISRSEFSDLSVKEMHTIE
                     AIGLSGDLNSAQVAKRLDITPGTLTVSIQNLVKKGYVNRVKSETDRRVVKLALTKRGKL
                     IYRLHHKFHMRMVEESLTGFDPEEAQVLIRGLRNLHEFLNDLKNQQRKE"
     gene            20077..21021
                     /locus_tag="FE334_RS07845"
                     /old_locus_tag="FE334_07820"
     CDS             20077..21021
                     /EC_number="2.3.1.180"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /locus_tag="FE334_RS07845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07820"
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="WP_208965683.1"
                     /sec_met_domain="fabH (E-value: 4e-74, bitscore: 246.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKVVATGQYLPANRLTNEQLIAQAGIDSSDEWIVQRTGVKARRF
                     AEGESVADLATEAARAIIANVGPDVKENIRLIIVASMSSGNPTPSIANQVQANLGIAEA
                     WGFDISGACSGFTMAVDIAERMSRTYESGYVLVIGADKMSQILDLSDRTSSIIFGDGAG
                     GLLIACDGAGLPGYRSQLVALEDTKDAITLDKTVPDRQHLTMLGRDVFNFVVRAVIPGL
                     AKFIDELNESYDYVLSHQANDRLLDVMSQKLGIDRQQIPANIAEVANTSSASIPILLDD
                     LVQAGTITLSGQQQIVMVGFGGGLAYGINYFKL"
     gene            21061..21285
                     /locus_tag="FE334_RS07850"
                     /old_locus_tag="FE334_07825"
     CDS             21061..21285
                     /GO_function="GO:0000035 - acyl binding [Evidence IEA]"
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (3-69). E-value: 4.9e-15.
                     Score: 47.6. Matches aSDomain:
                     nrpspksdomains_FE334_RS07850_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773476.1"
                     /locus_tag="FE334_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07825"
                     /product="acyl carrier protein"
                     /protein_id="WP_004635068.1"
                     /sec_met_domain="PP-binding (E-value: 1.8e-14, bitscore:
                     50.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding_2 (E-value: 0.012, bitscore:
                     12.6, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVFETVKDIIVEQLGIDESEVTKETDLENGLDADSLDIFQIISDI
                     EDEYDITIDTDLNLQTVGELVDYVEQLIG"
     aSDomain        21070..21267
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FE334_RS07850_PP-binding.1"
                     /evalue="4.90E-15"
                     /label="FE334_RS07850_PP-binding.1"
                     /locus_tag="FE334_RS07850"
                     /protein_end="69"
                     /protein_start="3"
                     /score="47.6"
                     /tool="antismash"
                     /translation="ETVKDIIVEQLGIDESEVTKETDLENGLDADSLDIFQIISDIEDE
                     YDITIDTDLNLQTVGELVDYV"
     gene            21308..22279
                     /gene="fabK"
                     /locus_tag="FE334_RS07855"
                     /old_locus_tag="FE334_07830"
     CDS             21308..22279
                     /GO_function="GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="fabK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635066.1"
                     /locus_tag="FE334_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_07830"
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="WP_112779032.1"
                     /transl_table=11
                     /translation="MKTAITELLGIRYPIIQGAMAWVADADLASAVSNAGGLGIVGTGH
                     DSREVVKEKIDKMKKLTDQPFAVNAMLLNPHIEEVIDYIIEESGVKIVTTGAGNPSQYM
                     KRFQEAGIRVIPVVASVALAKRMERIGADAIVIEGMEAGGHIGRSTTLTLLPQVVEAVD
                     IPVIAAGGFGNGESLAAALMLGAEAIQVGTRFVVSKESNAHQNFKQKILKANDIATVVT
                     GQITGHPVRVLRNQLTTDYLELERLQTSEDEPDFSEMEKLGKGALRRAVVEGDIKQGSM
                     MAGQIAGLVKKEETVAEMIQDYIDGAQQAYRRCQACFTQDDA"
ORIGIN
        1 cctaaagctt taatatttat agtaaataca cttgtaaccg ctttattttt gtggtagggt
       61 gtagttgtaa ggagaatagg ggcacaaaaa tatgttgggt atttctatat gatataaagg
      121 gaggaaatag ttatgcgtaa gaaatgtaaa aaagtagtta ttctaagtct ggttgcatta
      181 ataagtttgg taactgtatt tagtaataat tatattcaag tttatgcaca cgatactggt
      241 gttgtagaga ataatgagaa gggatcatca gagctcaaaa aatctttaga aactgtagaa
      301 caatatattg atatggacgg tgataagaaa tttgatgaat cattagcgag agaagataat
      361 gcaagtaaag acactattga aacaggaaga atttacaatg aaatggttcg attagaaaac
      421 caaggagaac atgatgcgct tgtagaacaa cgaagatcat ggatcagtga agcaactaag
      481 tacggtaatt ggtgcggtaa aggagataaa ggtgctccgc ctatcgattt actagatcga
      541 caatgccaaa aacatgataa atgttatgct gcaaatggtt gggggaatac gcaatgtgat
      601 ataaactttg tgcataatat tgggagaaat ttcgactcaa taaaaaatat tagtgcttat
      661 gccaagaatt atgcaaaagc tgctgtattg ttatttgcag gaaaagtagg aggaactcaa
      721 gcattagcag aaaagtttcc tgctctagct ccatatttac cttaacatat taatgaacgt
      781 caatgtatgg attattccaa agttaggtta ggtgatttaa gatgaaggaa aaatggataa
      841 aaaagtggca aaacctgtct ttcctaagta aaatagctca tctgtcagta attatgttgg
      901 cattatttgc accttttgtg agtttagttg tagaagggtt attcaatatt tatattgtgg
      961 aaccaaatca tattttcaag tttttttacc taacattagt tttatgcgtc attgcaagag
     1021 aatggaagtt attgattgga acatttatca cattttatgt tgtgtttatt ttactcgttt
     1081 ggtatggatt tgtatttggg tgataaggca tcatgaaatg attcgcatag taaatataga
     1141 gaggacagtg agataatcat gaatatattt gaaggatcat ttggcgtatg gttgcccatg
     1201 atcataacat tagttgggta ccttctgaag tttgtaggat tgtatttggc tatctatttc
     1261 ggcgttaaac atggattgaa agcgtctaaa gaaaaagcaa gcagttctac tgatttcaaa
     1321 taaagtttaa gctgcacgat taaatgttta aaccacacag cttaatattg ctagtaaatc
     1381 ctagtgcccc tataattatg atgtaatacg ttattagaga agccttgttt tagggcttct
     1441 ctttgattcc gtcaacattg atacagctag atataactca attcagtatg tcagcaaact
     1501 atttgaaatc cttatacccg agtgttacta ctcgcttatt ctcgaataat atcatcaatt
     1561 tttttatttt tctctatact cttcataata aaatacgtcc acaactcctg accaatccaa
     1621 gacacacccg acaaaatcaa caatatccaa gtcggccatc tcatacacgt caccatcatt
     1681 gacactaccg gtagcgtctt cgatataatt aactttaatt ccgttgtgtg tcaggataat
     1741 ggcactaaat aagcaacaga attcggcgtt aaagccacaa atggccactt cgtcaatatt
     1801 gtgttcttcc agtatctgtt ggagttctgt ccccgaaaat atatcaggtg ttcgcttctc
     1861 aatgatgtaa tcactatgat ctttagcgac actgaccaac tccgcacccg tgcttccttt
     1921 gtaaatcgaa ctttcttcat tgtcatcgat gtgcctgaag gaaattattg gtttatcttc
     1981 tctagagaaa tcatcgacta aatattgaat agtatccaac atctgcgagt aatcaccgtg
     2041 tttcaacatg ccttcttgta cgtctaaaat tattagagct tccatattaa ttccccattt
     2101 cattcattta gcttcattat acactattct tatctcaggc gtctattcaa tccaacaagt
     2161 catcaagttc atcaatttca ccggcatctt tgctgacata gtagtagatg tactcttttt
     2221 cttcgtctgg cagtgtcaag agtaattgcc gttcaagttg tatgatgtgg cggtcaatgt
     2281 attgtttcat gtcttcggtt aagtcagtag gtaattcgct agctgattca atcgtggagt
     2341 tcgtcagaat tcgctccacc gccccagcta agtggaggga taagttgaca tgaaaagcga
     2401 atcccttctc ctgcttttgg cactcttgaa gtctttcagc aaaatcccaa agaatgggcg
     2461 tgatgcgtgc tgggttcaaa taaagatgat tatcttctat atagcgctgg acaagctgct
     2521 gggcttgggt ttgatcggtt atctcagtcg ggtcatgagt acctaagagt agttggttta
     2581 actgtgatgc ccccatatta ataaaggtct caagtggaat aaatggtgca gcgatttttg
     2641 ggtcgataat gccggtcgaa gcgataatat tgtagcgact taagacactt ggtaagtgct
     2701 cctgtagatt cacagcagac attacaatga cttgatacgg catgtcagct ttatttgcgt
     2761 acggtttttc cacgatttct ttgatttttt tagcagttcc ttcaccactc gcacagatcg
     2821 ctaaaatagc cgattctttt aagtgattcg gtgaagttgt cggttcggcg ttaatttgat
     2881 gataacccgt aaagtgttta agtgactggt gtagttcatc aagtgtggtt gtggtcattg
     2941 atgctttgcg gaccgcttct aagacgattc cggtcgatac catgtcaaga cttctcacct
     3001 ttacgccggt ctcgagttct agtttagtcc caaatgtcgc tagcgatccc atatcaacta
     3061 ataacaaaac accacttccg tcatcagcgt gcttgacggc ttctttgatc ttggtgtagg
     3121 cttctgttgg ggacatcgat aagggcatgt cgatagctgg gataggcggt gtttctaaga
     3181 gttctgccac cactttagcc atgctgctgg cggtagacgg accgtgtgcc gccagaacaa
     3241 tcccaacttg ttgggttata tctgttttaa aagatgtcag caacacggtg agatagtacg
     3301 cttcggattc gggaacagct aagttgaagt ggtcagcgat gcggtgcctg ataacttctg
     3361 cctggcgtaa ggcatccggc gtatttaggg ccaggtcacg tacgcgcgca ttgaatgggc
     3421 gcaattgtcc gtcgcgcaat tttcctatta gggagctaat gtgtaggctt agggcataga
     3481 gtaggctacc gctctgtcca ggcgcggtct ctaagtcggt catagccaag acatcgcggg
     3541 caaaatcaat aatctcaagg ctgacgactt cggccagttt ggaggtggtt tgaagcgcaa
     3601 agccttggtt tttataaaat gattttagat gtaagttgat atcggttgag atatattggt
     3661 tgattgtttc ttgatcgatt tgctcttctt cgaggagggt cgctttatca gcgataatgt
     3721 catagatatt atacggcagt tcataactat cacggtctag aataatgtcc acatcttcgg
     3781 gatggatctc aagagtaggt tctaaccggc gaatgagctc actactggca gaccggtcgc
     3841 tcgtcatggc gataaatgaa cgtgttaagt tttccggtag ttcgtgggtg gtgagttcaa
     3901 ttacattatt atgcagctgg ttcataaatc cgcgagcaca gaggagttgg atgtttgatt
     3961 taagctgtcc gatattgcca taatcaacat gcgaaatcaa tgtacgaacg acatccttgc
     4021 tcatggagac tttgcggtta atgcgtttgg cttcgaggcc gaccattagc tttaataaat
     4081 caatctgttc agagactgga cgctgcttga acgtagggag ttggatatta atcgggatgc
     4141 gacgaataaa ggtttcgagt aaggttgact ttgggtcttc cgtggtggca cagacaattc
     4201 ggacggaagc atgcagcttt cgatcggctt ctcccaagcg tgaatattgg cccgtatcca
     4261 taaagtagaa gatcatctct tgcccctctg gtggtaagcg gtggacttca tctaggaata
     4321 acattcctcc atcggcttgc tgaatgagac cgtctttttc tgttgttgcg cccgtgaaag
     4381 ctcctgccgt ataaccgaat aagtgactca tcaacagctc ggggttcttc gcataatcgg
     4441 cacagttaaa gacttgtagt tccttatcgg ctgcgatgag tccttgattt ttggcaaatt
     4501 gaaacatcgt gttagcaaaa aaagattttc ctgaaccagt tggcccggtt attaaggtgt
     4561 ttaatccatt cggtggataa agaatcgatg cttttccttg catgacggca ttcttcacgc
     4621 tgccattatg accaatgact tgcgagaaga catcacgctg attgtcacta gtcatttttg
     4681 atcttggagg agcggtgtct gccggaatag gggaagagga gctcggttcg ttcaaggtat
     4741 atttgacggg gcgtccgcct aatttggtta agcgaccttc tccagtgagt tcgttcaaat
     4801 aacttgaggc attactgcgc tgaatgccta atgcttcggc cacttctcgc gtggtgaagg
     4861 gtttctcagg ctggtttgat aataagtgta taatttcttc tttccgctcc ataatagact
     4921 ccttcttagt agtgtataag tgatatacag atattataca caaaataaaa aaatctgact
     4981 agacctatgt ggggaataag tctagtcagg atgtcatgtt cgggtttccg aggaataatg
     5041 agttttatca ttaatagttc acttggcgtc atctaatttt ccgctcgcgg gtggagggga
     5101 tgccgagttg actgcggtat ttggcgattg tgcgacgaga gagtgccatg tgtttagttt
     5161 gtttcagtgt gtcagcgagt tgttggtcgg atagtggatg agttttatct tcaccctcaa
     5221 ttaagtcact gagagcagtg aagatagcat cttgcgaggt gtctttaacg acggaacgtt
     5281 tggataagag actttttagt tcgataactc cccgattagt ttggacatag gtgccacgaa
     5341 tggctcggct aatcgtggac tcgtgtagtt ggagttgctt gactaaatca ttaatttgta
     5401 atggctgtaa ggaggcatct tcagttaaga aatagtcctt ttggtatgat aagatagcag
     5461 tccccactcg aaggattgtt tcttttcgct tctgcagagc ctgttgtagt tgatcgaatt
     5521 cttgtttttt tgctttaata tagtcttgga tgcttggttt gtcgatctgg gctagttcat
     5581 cgtagtaaga ttggtcgaag gtgagcagtg gtgtctggaa gcgcgtttcg ctaattgtaa
     5641 gctgctgatt agccactgtt acttctagct cagggtaaat catttcagtc tggggggctt
     5701 gatataagtt ggctgggatt ggcgatagcg tttggataaa tttaaagata gcgcgaatat
     5761 cagccaaatc agttctgtaa atcgttgcta agtcactcca ttgacgattc actaatcggt
     5821 caaaattttc ttctaaaata atataggcta agttgggtgc actgtccaag cgctcagttt
     5881 ggagcattaa gttttcgcgt aggtcgcgag cgccaatacc aggtggatcc aattgttgga
     5941 gtaaggttag accatcaaga accatatagt cactctcacc cgtgatttgg acggcttctt
     6001 ctagtgtttt cgtgacataa ccattcgtat caagctgctc aatccaccac atcgtcatat
     6061 cgcgaagggg tgtttggcgg tagaataatt ccacttgttc ggtaatgaag tcgaataagg
     6121 tttgggtgga ggcgttgatt tcatcaagat tgatcccttc tgcgcttgat tcactgccaa
     6181 acattaaggc atcatcgttc gcgaaggtca tcagtgggtt ctcactcata taattgctaa
     6241 tgtaatgtga taagtctacg cgattgagtt gcaagattcc aacactttta attaaaaagg
     6301 ggagaggagc ttgcgtttgt ttgatcattg cttgttgggt aattttcata ctatgccttc
     6361 tctcggttcg gtgcgtctaa cgtatgatta tcaaagggat aggttaagtt ccattcttga
     6421 cggatggcat ccatgagttc gatgacatgt gtggtcttcg tcaggtgagg ctgtggcgtt
     6481 ccagatgcaa tcaagttggt gatagcgatc atttcctctt ggagtgcttg actggattgg
     6541 ccggcttgat aatgttcaat cttcccttct ggcgaggtga aggtgatgcg atctgcccgt
     6601 gggtaattat caatttcgaa atagccccct tcgaaggcaa tcaccccacg cttcggtaat
     6661 ttggcgtgaa aggtcatggt aatggttgct agttcattct gatcattttt caagacaatt
     6721 ccagctgatt catcaactcc gaattgttca ttgatgtaac cgagcgtttt catctcagtc
     6781 ggtggtgtgc ttaagaattc catcacaaaa ttaagggcgt agacaccaat atccagtagc
     6841 gctcccccgg ccaagtcttt ttggaagaag tagcccgtat ctttcggctt gaagcttcca
     6901 aaatttactt ggatcatctt cagcgcaccg agctccttct ggtcaatcag ttcacggatt
     6961 gtctgataaa cgggacggta atggattgtc attgcttcga ataaatacac gccttgcgcc
     7021 tcagctaatt gcttcaactg agctaagtca tctgcctgag tgacgatcgg cttctcgaca
     7081 acggtatgtt tgccggcttg cagggcgtca gctgcaatct gagcatgggt atggtgaggg
     7141 gtagcgatat agacaatatc aatggcctca tcctgaagta gctcctggta atcaccgtat
     7201 gcagcatgca atccaaaggc ctccttgaag gcttctgcct tttctaatga acgggaagat
     7261 acgccatggc acgttatgcc ggtatcttga aatccagctg caaaatcttc agcaataccg
     7321 cctgtcccga taatgcccca ttttaattta ttcatcttta ttcctacttc ctagcgctag
     7381 ttaaatggtg gatagacttg aatcaatacc ttcatcataa agggtttccc tctgatttgc
     7441 aagcgatgtt ttctgacaaa aaaagccact ccctaaaggg aagtagcttg aatataatct
     7501 attattcagg gttggaggca atcatatcga gatctccgga taaggtgacg gaagagacgc
     7561 cgtgagggat aatgaaggat tgggccttct caactggata agattcccga tcaatctcaa
     7621 tcataccttg gccatcgagt accgtcacta gtgtataatc accggttaaa tcgacaacaa
     7681 attggtcacg aatttgccaa cgatagacgc tgaaatattc attggttaaa taatgcgtaa
     7741 tcgcactcat tccgacccta tcttcctgct gctggatagc tggatcgatg tgcggaatgt
     7801 ttgaaacagc aatcgagtct tcaagatgca attcacgtgt gtttccatct ttgtccgtac
     7861 ggttatagtc atagacacga taagtggtat cagaattttg ctgtgtctct aatatggtga
     7921 tgccgccttt aatcgcgtga atcgtcccgt gtggcacata gaagaagtca cccgctttta
     7981 ctggaacttc gcgcaagagc tcatcccact cgcctgcttc aactaagcga cggaactctt
     8041 cttcggttcg cgcattgtga ccataaataa tcgttgcatc ctcatcggcc gaaatgacaa
     8101 accaacattc cgtcttaccg agttcacctt catgttcttg gccgtactca tcgtccgggt
     8161 gtacttggac agacagatct tctttagcat ctaaaatctt agtcaatagc gggaacgtgc
     8221 ccggttcgtc gacaccgaat aattccggat gctggtgata caactcattc agccccatgc
     8281 cggcgtattg ttcgggagaa gaaacggttg actcgccatg gggatgggtg ctgatcaccc
     8341 aagcttctcc gaccgtatca cttggcagat tgaagccgaa ttcagtttgt aatttcttgc
     8401 caccccaaat tttatcctgt aaaactgggg ttaaaaagat tggttctgta ctcatctgtg
     8461 acactcctta tttaatgata gattttcatc agttatcccc ataatatcga aaaatgttgg
     8521 taaaggctag tgagatcaat tgtcgtttta ttaagtgggc tcagaattgt cttgagtctc
     8581 tagaaaactt tggacgttcg ttgcttctgg ctcagttagg aatgcttgtc ctttaaacaa
     8641 gtcttctagc cggtcgatgt caatccccaa gtcaacggat aagtgttggg gggtgagttg
     8701 cttctccgcg gcaatgatgt caacttgtcc cattaggtaa aggctgtcta attcttcatt
     8761 ggacatgtcg tcttcgctta gtgctaataa ttctctaagc gtattgcggt ctaaaatttt
     8821 tcctaagttt tgaatcatct gtttaatttg tcgcatcaga aaatcgcggt cttcaatcat
     8881 gagaggcctc ctagtatgat aagtttatag gtcacaagac ttcataaata aatcataata
     8941 attattgaaa tcaaacaaat tattttcttg ccaatatcta ctttctgaag tatcttgtga
     9001 atgatacata tttttatagg tttcaaattt tttttcatgg aaatcagtaa tattgaattg
     9061 cttagaacga atttttcgca taactttttt agcctcatat tgaatgatgc tatctataac
     9121 agttttgttt tgtatactcg tactaagttt tactgatgat tctatcgagc atccaagttg
     9181 tgcaagtatt ttatctagat attccgaagt gaaattaaca ccattactat gacaactact
     9241 aatactgata ccaacttttc cccgaagttt taataaatgg gtccagtatc ctattctatc
     9301 tataaaattt ttcataaacc cagaaacatt atgcatatat gtcggactag caaaaataat
     9361 aatatcgctt ttgataagct gagattcaag ttttttcata tcatctgtag catctgtaga
     9421 gtgaccgtgg taaaattctt ggttgtttcc ttgattataa tttattgtaa gagaatttcc
     9481 atttgtataa aataagttta tttcaacatc tgaatgagca ttcaaatggt caacatacct
     9541 atgaattgca aattcacaaa tagaagaatc gctaagacta ccacaaaaag caaaaacatt
     9601 cacaaatacc cctcctcata aatatatctt atagaaaaat agaataaaag tcaataaaca
     9661 tttgaaatca tcataaaaaa atattataat gaggatgtgg atgagccatt aggaggaaaa
     9721 tatatggatt ttgaaatgga aaagtcattt gatgagtctc aattaaaaaa attagcaaaa
     9781 aatacaccat tggtaaaaga atctgaaata atagtggaag tttcggcgat agcatgctcg
     9841 ataacagtgt cgattttatt atcacctact tcagcattgt cagtgacggt tacagccggt
     9901 gactaactag tacaactaac aactaaaaaa taggcaggct tatccactat acatataaca
     9961 tataaataag atttatagaa agaaaggata taaggatatt atgataaaag aaatcaagtc
    10021 aaaatttact aatgatattt tagcaattat tatgaggaaa gtagacgaca gtataagtcc
    10081 tgcacaccta gaatattttt caaataagtt gatagaaagt atttcacgtg acataacaaa
    10141 ttcttttcaa gatcaaattt ttgatgtaat aattgtggaa tttcataaat tcagggaaca
    10201 caataacttt aaagatgtgg atacaactat agatgaattt tcatcgaaaa ttgatagaga
    10261 gagctttgta gagaaattat tttttgaata tccattatta gaagaaaaaa tattaagtag
    10321 aattaaggga gacacgtctt ttataaataa aatgctatgg gatgtaagaa gagaccaagt
    10381 ggagttggaa gaagcattca atattgatat tgaaacatta tgtaatgtat atattaatat
    10441 gggtgacaag cacgataata aatctgtcac acttttaaag tttaaaaaca agaatatact
    10501 gtataaacct agagagattg tattcgaaag tactgtagaa actttgattg attctattac
    10561 tgaaagtaag tgtttaaaac aagagtatat tagaagagta aaacaacctg atagaatcat
    10621 tggaaaaggt tattattggg aagagtatat acatgaagaa aaagtaaagg aggaggacat
    10681 agattcattt tattatacat caggagtata cttagctcta ttttacattt taaatattac
    10741 ggatatccat ttcgaaaata tcttattaaa atctgactac ccgatttttg ttgatttaga
    10801 agtagggaat ataaaatttt ccaagtatga gattaaaaat aaccatcttt taatgaataa
    10861 cagtgtgttg atgaccggtc ttttgccaat ttatgataaa aagttacagt taaatatgag
    10921 tggtcttttt tcgcatcctc aagagacaaa tgaattatca cgccaggtac caaaatatga
    10981 taaaaaattg ggtattgtat ataaaaagga ggcaggtaaa atcatcccta aaaatgctaa
    11041 tttatctcac aacaacaaaa aaatagcaat aaattatttg ttgaagggca tgtctgattg
    11101 tcttcattat attcgcacac ataagcgaga tatagtgaag caaatagatg agtatgatga
    11161 ttttaagtac agagttcttc tcagacacac aagtgtttat ttttctttta tagaagcact
    11221 aaatacccct aaagcattaa gggacaaaaa acaaagagaa aaaatattga atattttaga
    11281 agataattac attcctaatg acggtgtggg atatgttacc ttatttgaag aaatagctca
    11341 attaaaagaa ggaaatatac caatctttaa agttccgttc aagcaaagag gtctatatgt
    11401 tggggatgaa gaaatactta acgagtatgt agacacacct ttaaaagaag ttgttatttc
    11461 acgaatttcc tgcttagata atttttgtat agactatcaa ctgagactaa ttaaacttag
    11521 cattgcttcc acagttccag taacacaatt aaatacttta aataacgttg agcatttatc
    11581 ccaccaaaaa gttgctgatg atgagttgaa acaaaatata ggaaatctct tgaacaacat
    11641 aggaagcttt tcgtttgaat tggaaaatgg gggtgttgaa agatatgact tacaatatgg
    11701 agaaaatgct tttttagaaa tagcacctca gaagtttaat ttatatgata cattagctaa
    11761 tattgcttta ttctatgtat ttgggaatta ttttaatgaa ggaaaatatg aaaaaattgc
    11821 cctctcaatg tataaaaaca tttctgaaaa agttaaatat attatggaat ctgactccaa
    11881 aactaacgat gaagaagata tatctgtgtt tgaaggaata tcaggagtaa tgtatgtgtc
    11941 atattattta tacactatta ctcaaagtga aagatatctc gaagatacgt gtgaaatata
    12001 taaatttgtc ataagaaaag taaaagagaa aatctcacat aagaagatga atttagatta
    12061 cattagtggt ctttcgggaa ttggattatt attgataaat attcttaagc ataatagcaa
    12121 agtagtgaaa gagtcagatt tgatgatgat aaaaggggag atagaaaaaa actatcacac
    12181 tattgaaaag aatggtttag cgcatggaaa actcggacca ttgctgtttt tgatagaata
    12241 taatctatgt gtttcccgta ccgaagatga aataaaaaat attggagata gattattggc
    12301 tctctctcta gatacagaaa ataatacatg gtgtaatgga ctatctggat atttatatgt
    12361 tttaaacgag tataaaatat tgttgaatga taacaaattt atagtccaga caatcaacag
    12421 taagataaaa accataatag ataataatat attaaaaaac aaatcattat cactttgtca
    12481 tggtgtattt ggcaatatag aggtgttact aaaaatcaat gaaaatataa atatagatag
    12541 agaattaaat aaaaaaatca atttaagcta tattaaagat ttgacgaaaa taaagtggtg
    12601 tgatttggtt gatgttactg caagtggttt tatgacagga gcaacgggca tcgcatatga
    12661 attaatgaga attagaaaca aaaaaatacc atctataatg tttttagaat tttttgatga
    12721 taaaggggtt aaaaactatg agtaatccag ccgttagctt ggaaaaagta aaaaaacagt
    12781 ttaagaacaa atatgcttta aaagatataa atatgagtgt agagaaagga gaaatatttg
    12841 ggcttttagg tccatctgga gcagggaaaa ctacaactat taaaattatt acaaaaaagg
    12901 aaacacatga ttacggaaga gtaactatat ttgggaaaaa gatcaatgat gtaggtcaag
    12961 acttattgtc tagatttggt atagttagtg atgatagtgg gtattataaa agattagatt
    13021 tgataaccaa cttaaaaata catgccaaat tatatgatcg atctgatagt tatgtagaaa
    13081 acttattaaa aaaaatggga ttatttgaag acaggaaaac aaaagctaag aatctgtctg
    13141 taggtatgag gcaacgaatg tttttagcac gagcacttat caaagaacca gtaattttat
    13201 ttctagatga gccaacaagt ggtttggatc ctcaaacatc taatgtaatt catcagctta
    13261 tattagatct taaacaaaat ggtacaacag tttttttgac tactcatgat atgaaagaag
    13321 ctgagaaact atgtgacact attggattgc ttcacgaggg aaagatcatt gaaaaagaca
    13381 caccacaacg tattataaat aagttcgcta cagagtctga taatgatatg aaagttattt
    13441 ttaaaaatgg ggatgtaaaa tttatgaata ccaattcttc ggaatttgaa tcaattaata
    13501 acgacgatgt tatggatatg tatagaattg aactaacctt agaagatgta ttcttaaaat
    13561 taacaggaga tgagttagat gtttaagcgt tttaaaaata ttttatggtt aagaataaag
    13621 cagtatatct ctaatccaat gtatattatt ttaatagcac taccatatct gtttctgtgg
    13681 ctatatgatc ttctaattgg ggatatggta ggagccgatg accttttgtt tacagtttta
    13741 ccattattat acacattgac aatgggacaa ttaattagtt cgattgtttc tgaagaaaaa
    13801 gaatataata atcttatgga actacaatat tcaggagtga aaggaccaga atatatttta
    13861 agcactatat tatatccatt attactcgga atacttggta ttgctttatt gccaatggtt
    13921 attggaggga tcagtgagat agatatcagc tatttgagtt actacaccat caatttctta
    13981 acagctatgt gttttgctct gatctatatc tccattggta tgttctctga gacggtcaat
    14041 caaagtcaaa taatcgccat tcctattatg attgttacat tatttttacc tatgggagca
    14101 gcgtataata atactgttta tgaattcact agattttcgt ttatgggaag ttttgtagaa
    14161 ctatacaatg acagtggaaa tatatttggg agcacctcct ttatttcatt gttaatttgg
    14221 atcgttggat tatttgtgtt agcacttgtt tcttttattt atggatttag aagacggaat
    14281 tggtaaagtc tattttacaa tataatagat aaagcaagcg atttgtaaac ataagagctt
    14341 gacatatcaa ttggcgacgt aatcaagagt atcggaaaga gtatcggaaa cagcttaaaa
    14401 atgcaaacga gagctttata aactatttgg aagcttgttg accggcctga atcattagga
    14461 tgaattattt gagacagacg ggatagtggg tgtggttttg ttactcaatc ttgtgaggca
    14521 cggaagcgcc gtacagtatg gacatgttcc atcaagtagt tggcggcgat tccaatcgcc
    14581 attccgctca gaatgccgaa gaaacttaag acgggcaggt acattagaac agtccaagtc
    14641 tgggcgataa aactagcaac gagcaattgc ccgagattat gaagcattcc acccgttgca
    14701 ctgacgccga taatactgac ttgcgagcgt ccgagcgtct tgaccagcag catgccaaga
    14761 taactgagaa gggctcccat gaagctgtac ataaaggttg atagtgtccc gcctaacagg
    14821 gtggtcatta atagacgcat catcaagacc ttgaagctat ccttcaaggg taaggtgtaa
    14881 atagcgataa taacaattaa gttagctact cccaacttcg ctcccggcgc gaaggcgaac
    14941 gggaagggga tgctattttc aaccagacct agaatcacgg cttgggctga taataatgaa
    15001 atataaatca tacgttgtgt ttttgtcatg aaaaatcgtc ctttttagtg gttctttcat
    15061 tttctcataa ataaacagtg agtgctagtg attccgctag ctttctaaga tagaatggag
    15121 aggatacgac ataaatagat gaaatgtgtt ataatggcgt tgggaggtag agatatgtca
    15181 gatcattatt atacaaaaga gccgacatcg acaagcaatc cagaacaatt taaagaagtg
    15241 gtcatcggtc agactcttaa cttaacgtct gatgcggggg tcttttcgcg tggacagatg
    15301 gactttggga cacgaacatt gattgaagcg gtgcagacgg ataagaagtg ttccgggccg
    15361 ttgttagata tggggtgtgg gtatggagtt attggtattg cgctggcagc agctaatcca
    15421 gagcgaacaa ttcatatggt tgacattaat gaacgagcag tcgcactagc taagcagaat
    15481 gctgaagtga acgcggttga gaatgtcatc attcagcaat catccttatt cgagaatatt
    15541 gtggagcggt cgttcagtgc tgtggtatct aatccaccga tccgagcagg caaacaagtt
    15601 gtgcacggca ttctagagca gtcatatgat tacttgcagc caggtgggaa actctacatc
    15661 gttatccaga agaagcaagg cgcgccgagt gcgaagaaaa aaatgcaagc cgtcttcggt
    15721 aatgtggagc gaatcgcctt ggagaaaggc tactgggtct taatggctga aaaatagagg
    15781 ggcattttaa acgataggat gattgatcac tgtttcagga aaattatatt tttttgattg
    15841 taggggttgc aatatccgga aaagctggta taatagtaac tagattggtt cattagctca
    15901 gttgggagag cactagctcg acaagctagg ggtcgctggt tcgagcccgg catggaccat
    15961 aataatagcc acctgttatg gacaggttga tagtctatct atgaatgata taggtaggct
    16021 ttttttttat tgagaaagga ggtcgatcga atcgatgaga ttatggcacg aaacattaat
    16081 tagtgatttg ccccggcagc aattactcgg gcaacatcgc gaatgttgtg cattacgtgg
    16141 gaagggatgg aatcgtccgc acgctacggt tcaatatgtg ttcgattata gtccgtataa
    16201 gttatatcag tatcatcagc tcattatgga ggagatgaag tcgcgcacgt atcagccaga
    16261 tgaacgttgg gaagatccat tatatcgggg gaaagcctgt gacccttatc gtgaacttga
    16321 accagtcaca cctacaaaac ccatttatcc tgaacataat gcaacttatc tagcagaatg
    16381 tttagaaaac ttggctgaga agggaattga gttatcagtc agtgaaagtt aagaaaccta
    16441 tttgaaaaaa tatataataa aggaggttat cccatataga aggataacct cttattcatt
    16501 aatcagttaa gttgacacgg tagtattttt tcttgccacg acggacgata atgaagcgat
    16561 catcgaaagt atctgtgctg gagatggtgt agtcaagatc agtcactttt tcaccactaa
    16621 ttgtaatcgc gccttgttcg ataaattcac gggcttcccg gcgagagcta cagatgccgg
    16681 tgatatcagc taagaactcg acaatatcat gttcaccacg cgttgcttca gaagttggca
    16741 cgtcgccaaa tgtttcttca atttgttcag ctgttaagtc ggcaatttca ccggagaaga
    16801 gtgcttcggt aatttggata gcttcatcga gtgctgcttg accgtgaaca aatcgtgtca
    16861 tttcttcagc taaagcttta tgagcttggc gtttccatgg ttcatcggtg aggctctgtt
    16921 ccagtttggc gatttctgtt tggtccaaga aggtgaagta tttcaagtat ttcacgacat
    16981 cagcatcttg ttggttgatc cagaattggt aaaattcgta agggctggtt ttttcaggat
    17041 ctaaccaaat ggcaccacca gctgtttttc caaatttggt accatctgat tttaacagta
    17101 gtggaatggt taagccatat gcttttgctt cgtggccttc ttttttacgg attaagtcaa
    17161 gtccagccgt aatgttcccc cactgatcac tcccaccgat ttgaagttga acatcttcat
    17221 gacggtataa gtgcaagaag tcaatggatt ggatgatttg gtaactgaat tcagcaaatg
    17281 aaatcccttg ttctaggcga ctagcgacga catcttttgc cagcatggta ttgatattga
    17341 actctttccc gtagtcgcgc aagaagtcca agaaactcat cggactgagc caatctgcat
    17401 tattagtgag acgaatgcca gattcttctt cccccgcctt gaatagcttg cgcatttgag
    17461 cacttagttt gtcaacattg tgctggacag tttctttagt ttggagttga cgctcagaat
    17521 ttcgcccact tggatctccg atagaaccag tcccgcctcc aattaagaca actggcttat
    17581 gtccagccat ctggaatcgt ttcaagataa tgaagggaat gaggtggcca atatgcatac
    17641 tatcccccgt cggatcaact ccacaataca gtgcaatcga ctgcttctcc gttagttcct
    17701 tcaacccatc ctcatctgtc tgttgattaa tggctccgcg ccactgtaat tcatcaataa
    17761 tattcatccg aattcctcct ataaaaagtt aaatcatgat ataaaaaaag cccgctaaaa
    17821 agctcaacac ttcttaggga cgactctagc cgtggtacca cccacattag gcactttacg
    17881 tttactgcct cactctagcc gataacgctg gctcgcgata catgtaattc actccggcat
    17941 atatattggc ttccaccacc gccaactctc tgtaaaaggg ataccttcgc tactaaataa
    18001 cacttctaag tatagcgatt aatctgcgtc atgtcaactc tattaaattt ggcactctga
    18061 tagctgatag actatcacat agttttgtgt cggcagaata aaaattaaac gctaattaat
    18121 gaaaatattt gatttaacta gtggtcatat tgaactaatc catgtcatta ggtgtcatta
    18181 gatagaagtg agctataaaa ataatgatta acgatttata ctttaagcat tgtcagtgga
    18241 tagagttgtg aagccttcta tgacaatacc aaaaagccaa ccacgacaga tcgggttggc
    18301 ttttttagac tctattttta tagtccgagt tctggtgctt tttcgcgagc gtcttggatg
    18361 aatttacgca aggtattgat cgagtggttc atttttcctt gttcgtaatc atttagcggg
    18421 atttcgatag cgcgtttgac gccgcgatcg ttaataatcg ctggtgtacc gatgtagata
    18481 tcatcatgcc cgtactcgcc ttctaataag acagagagtg gtaggacgga attttcattg
    18541 ttgaagatgg cttgggtaat ttttgccaga gctgctccga ttccgtaata ggttgcacct
    18601 tttcgttcaa tgatgtggta cgcttgatca cgaacagctt cgaagatttc aagcaattcg
    18661 ccttcgtcct cgagcgggtt tgtttcaagc cattcggtaa tctgcaaacc agcaatgtta
    18721 gcgtgtgacc agactggaaa ttcagtatcg ccgtgttcac cgataatgta gccatgaacg
    18781 ttacgggcat cgatgttaag acgtttagcc agttcaatgc ggaagcgggc actatcaagg
    18841 gaagttccag agccaacaac tttgtgagcc ggcataccag agaacttgtg aacagcgtaa
    18901 gataagatat caactggatt cgatgcgacg aggaagatac cattgaagcc actagccatg
    18961 acttgatcga caatggtttt cgtaattttt agattcttat ggattagatc taagcgagtt
    19021 tcgcctggtt tttggccggc cccagctgtg aagacgacta aatcagcatc ggcacaatca
    19081 ctgtaatcag caacgtaaat atcttttggt gcagtgaagg cgagggcact gtttaagtcc
    19141 atgacatctc cttctacttt gaatgggtca agatcaatga ttcctaattc ctgaccgatg
    19201 ttttgattga cgagcgcgta cgcataggct gaaccgaccg caccgtttcc gataagaata
    19261 atttttttat gttttttctc tgattgagtc attaagacat ctcctacttt ctttagtcta
    19321 gttttattat agtcaatttt tatactaaaa atcaattgag agtcttttat ttggtgaaaa
    19381 tagttgcggt agcgcttaag ttattaagcg ggaattcaaa attgtacgtt ataaaaaata
    19441 aggttatatt ggcacatatg aagtgaataa gatgggaata aagtgtgaaa gttgtaaaaa
    19501 aatatagttt tctaaaaaaa attagcaaaa tagtttgaca atcaaataaa taagcgataa
    19561 aatttgagta tcaaatagtt tgataatcaa aataaaaagg aggtaaagaa tggatcatca
    19621 gacgatgaat gaggtaaatg agtacttggt gatgatcttt aatgaggtat tgtctattga
    19681 ggaggaggcg atcagtcgca gtgaattctc ggatttatca gtaaaagaga tgcatacaat
    19741 cgaagcgatt gggctctcag gtgacttgaa ttccgcccaa gtagcaaaac gcttggatat
    19801 tacaccgggt acattaacgg tctcgataca gaacttagtg aagaaaggct acgttaaccg
    19861 agtaaagtca gaaacagatc ggcgcgtggt gaagttggcg ttgaccaagc gaggcaagtt
    19921 aatttaccga ttgcatcaca aatttcatat gagaatggtt gaagaatcat tgacgggatt
    19981 tgatccagaa gaagcgcagg tgcttattcg ggggttgcgt aatttacacg agtttctcaa
    20041 cgatctcaaa aaccagcaaa gaaaagagta gttaatgtga gtaaagtagt agcaacaggg
    20101 caatatttgc cggctaatcg cttaacgaat gaacagttga ttgcccaagc agggattgat
    20161 tcgagtgatg agtggattgt tcaacgaacg ggagtaaagg cgcgtcgctt tgcagaaggg
    20221 gaatctgttg cagacttggc gactgaagca gcgcgagcta ttatagcaaa cgttggcccg
    20281 gatgttaaag aaaatattcg cttaattatc gtcgcgagta tgtcgagtgg aaatccgaca
    20341 ccgagtatag cgaatcaagt gcaagctaat ctgggtattg cggaagcttg gggatttgat
    20401 attagtggag catgtagtgg ttttacgatg gcagttgata ttgctgaacg gatgagccga
    20461 acgtatgaat caggatatgt actagttatt ggtgcagata aaatgagtca aattttagac
    20521 ttaagtgacc gaacatcgag tattatcttt ggtgacggag cgggcggttt actcatcgct
    20581 tgtgatggtg cgggattgcc gggctatcgc agtcagttag tggcgttaga ggatacgaag
    20641 gatgcgatta cgctcgataa gacggtgcca gatagacagc acttgacgat gctagggcga
    20701 gatgtgttca actttgtggt ccgggcagtg attccgggct tggctaagtt tattgatgaa
    20761 ttgaatgaat catatgatta tgtgctcagt catcaggcga atgatcgctt actcgatgtg
    20821 atgagtcaga aattaggcat tgatcgacag cagattccgg ccaatattgc agaagtcgct
    20881 aatacgtcaa gtgccagtat tccgatcttg ttggatgact tagttcaggc agggacaata
    20941 acactgtcag gccaacaaca gattgtgatg gttggcttcg gtggcggatt agcttatgga
    21001 attaattatt ttaaacttta aaaacgaata aaaacaaaca attaaatgga ggaattacgt
    21061 atggtatttg aaacagtgaa agatattatt gttgagcaat taggaatcga tgaaagtgaa
    21121 gtaacgaaag agaccgattt agaaaatgga ttggatgcag atagtttaga tattttccaa
    21181 attatttcag atattgaaga tgagtatgat attacgattg atacagattt gaacttacaa
    21241 acagtaggcg agttagtaga ttatgtggag caattaatcg gttaagagta taaaggattg
    21301 tgtgtttatg aaaacagcaa taacagagtt actcggcatt cgatacccaa tcatccaagg
    21361 tgcgatggct tgggtggcgg atgccgattt agcaagtgcg gtgtctaacg caggcgggtt
    21421 aggtattgtg ggaacaggac atgattcgcg cgaagttgtg aaagaaaaaa ttgataaaat
    21481 gaaaaaattg acggatcagc catttgccgt taatgcgatg ttgttaaatc cgcatattga
    21541 agaagtgatt gactatatta ttgaagaatc aggcgtcaaa attgttacga cgggggcagg
    21601 gaacccgagt caatacatga aacgtttcca agaagcgggg atccgagtaa ttccagtggt
    21661 ggcatcggtg gcattagcga agcggatgga acgaataggt gcggatgcga ttgttatcga
    21721 aggaatggaa gcaggcggtc atattggtcg ttcgacgacg ctgactttat taccacaagt
    21781 tgttgaagcg gttgatattc ccgttatcgc agcaggtggt ttcgggaatg gcgagtcatt
    21841 ggcggctgcc ttgatgttag gagctgaagc gattcaagtt gggacacgtt ttgtcgtatc
    21901 gaaagaatcc aatgcccacc aaaactttaa acaaaaaatt ctaaaggcaa acgatatcgc
    21961 aaccgttgta actggccaaa ttaccggtca tcccgtgcgt gtcttgcgta atcaactaac
    22021 gactgattac ttggaactgg aacggcttca gacaagtgaa gatgaaccag atttttctga
    22081 aatggaaaaa ttaggtaaag gcgcgctaag acgagcggtt gtcgaagggg atatcaagca
    22141 aggatcaatg atggctgggc aaatcgctgg tttagtgaag aaagaagaaa ccgtggcaga
    22201 gatgattcaa gactatatcg atggcgccca acaagcttat cgtcgttgtc aagcgtgctt
    22261 tacccaagat gatgcgtaaa atgtattaga tgaaatgtct gaaccaggcg ataggagaag
    22321 gataagaatg aaggtagcag tcatatttaa tggacaaggt gctcagttcg aggggatggg
    22381 tctagatttt agagaacact tcccagaagc acgggctgtc tttaaccaag caagtgaagc
    22441 gacaggtctt gatatggtgc agttagtgag tgaggatttt tcggagttac gtcagacgaa
    22501 gtacgcccag cctgctattg ggacggtgag tttagcaatc tgggcaagca ttcgagaaac
    22561 tctaccgcaa gttgattata tggcggggtt gagtttaggc gaatatgcag cactaatggc
    22621 gagtgggatt ttcacggtat ctgatggatt acgcttatta tttgaacgag gtcaagtgat
    22681 gagtgacgtg tgtgtagata ttgccgaaga tgaaccgatg caaatgttag ctgtcattgg
    22741 tatgc
//
