LOCUS       NZ_CP040411            10297 bp    DNA     circular CON 23-APR-2024
DEFINITION  Dolosigranulum pigrum strain KPL3256 chromosome.
ACCESSION   NZ_CP040411
VERSION     NZ_CP040411.1
KEYWORDS    RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 1941401)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Eslami,S.M., Gao,W., Bomar,L., Fernandez Escapa,I., Roberts,R.J.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     The nasal commensal Dolosigranulum pigrum exhibits stable synteny
            and highly similar strain circulation over a 20-year span
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1941401)
  AUTHORS   Flores Ramos,S.Z., Brugger,S.D., Skeete,C.A., Cotton,S.,
            Johnston,C.D. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-AUG-2018
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 530x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017655645.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 02:25:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,851
            CDSs (total)                      :: 1,781
            Genes (coding)                    :: 1,758
            CDSs (with protein)               :: 1,758
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 9 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:41:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 344186
            Orig. end                         :: 354483
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    677..690
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.5"
                     /tool="antismash"
     gene            917..1513
                     /locus_tag="FE334_RS01630"
                     /old_locus_tag="FE334_01615"
     CDS             917..1513
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922444.1"
                     /locus_tag="FE334_RS01630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01615"
                     /product="response regulator transcription factor"
                     /protein_id="WP_004636437.1"
                     /transl_table=11
                     /translation="MKILLIDDHKLFSQSLALVLDQTTSDVQVDMINSEGELPDDLSEL
                     TVYDVLVLDINLDKGFSEDGFELAEQIQAVVDVPILMLTGFDLPVYEYQAHKLELSGFV
                     NKNIDTEDLLSLLKHVKDGGRHFTTENWFIDELTPRERELLGAIATGKKRKMIAEELYI
                     SERTLTNHMQSIMDKLEVNSTIEAIQKARELGYLK"
     gene            1641..1823
                     /locus_tag="FE334_RS01635"
                     /old_locus_tag="FE334_01620"
     CDS             1641..1823
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS01635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_01620"
                     /product="hypothetical protein"
                     /protein_id="WP_004636436.1"
                     /transl_table=11
                     /translation="MMKYAPYSLLALAGILVYLTVNRYITMHSINVVPLIGAILCVIFF
                     FVWRNHLKQQGYYDD"
     gene            2346..2690
                     /locus_tag="FE334_RS01640"
                     /old_locus_tag="FE334_01625"
     CDS             2346..2690
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FE334_RS01640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FE334_01625"
                     /product="hypothetical protein"
                     /protein_id="WP_111951603.1"
                     /transl_table=11
                     /translation="MKVECTAVERLIQRGVNIKHKQLPKICFWLGVVVFTVAVLLPTDL
                     FQSITWIEKILGELKPVGLATIFLLPIIGSVGVVGSIMNKSVLYGSLNVALILSFPLMI
                     LVSNIVQALF"
     gene            2767..2994
                     /locus_tag="FE334_RS01645"
                     /old_locus_tag="FE334_01630"
     CDS             2767..2994
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636455.1"
                     /locus_tag="FE334_RS01645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01630"
                     /product="DUF6290 family protein"
                     /protein_id="WP_004636435.1"
                     /transl_table=11
                     /translation="MTIVSVRLNKEEEKAINSYATLTGQPVSQLLKQALLREIEDKVDY
                     TIGVEALEEHEKDLQTFTIDEVAGELGIEL"
     gene            complement(3111..3740)
                     /locus_tag="FE334_RS01650"
                     /old_locus_tag="FE334_01635"
     CDS             complement(3111..3740)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152256.1"
                     /locus_tag="FE334_RS01650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01635"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_236654080.1"
                     /transl_table=11
                     /translation="MINQAWGQFSRGNIPKAKQLVDDYINEQATTDYNALNLLGYIYLE
                     DGKIDKAESTYQQWLQLSRKNHDIEQEHIALHQLAMVYRMREDYGQALELIQQEARIIE
                     QHFPNNLLKQSVNNYEQGYLRMKLQQLNEAVSFMAQSLTQAEQTDDLIAQACAQRGMGE
                     ICVAQHKPSLARKYFKRAIQLFEQGGDTIGAQEVQLLMSQLLTDKT"
     misc_feature    3810..3823
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.59"
                     /tool="antismash"
     misc_feature    complement(3832..3848)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.87"
                     /tool="antismash"
     misc_feature    3837..3850
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     misc_feature    complement(3838..3851)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.28"
                     /tool="antismash"
     gene            3874..4761
                     /locus_tag="FE334_RS01655"
                     /old_locus_tag="FE334_01640"
     CDS             3874..4761
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037898.4"
                     /locus_tag="FE334_RS01655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01640"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208957588.1"
                     /transl_table=11
                     /translation="MMIGPKMKARRKTLGLNQRELAEGICAQALISKIERESSSPSSKI
                     LQKLSDRLGVPVTYFLSDSDDGDIEAAKNKENYKRLETLLRAELMKGEYQTISMMMAIN
                     ADLIAACRDDYFINLFKWLQGVLYYRIDRNPDKALGLLKEIESQVADEYIELHIDILMS
                     IGMIYDELSDDERASDAYQEATFLLNQSISFKKRVHVLRNHALSLKNMDEMKEALKYVL
                     RAIEVLIKHKSLHMMGDLYLLKGNLFERLGDTEEATQTYEDAIWVFKTLNIKESLTVAT
                     VALHNILPNNKLNN"
     gene            5001..5297
                     /locus_tag="FE334_RS01660"
                     /old_locus_tag="FE334_01645"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850392.1"
                     /locus_tag="FE334_RS01660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01645"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_208957589.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.3e-18,
                     bitscore: 63.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTTNKLSKTLLAVAAIAALAAPVSAASRFVSGGVWTYGAGGGGA
                     FSNYYHGQRRHSSTVVSLSNSKSKKAWAKKEQTSKAFIPTKFGEKAAFYYGVD"
     gene            5389..7413
                     /locus_tag="FE334_RS01665"
                     /old_locus_tag="FE334_01650"
     CDS             5389..7413
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="FE334_RS01665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01650"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_004636433.1"
                     /transl_table=11
                     /translation="MIKKIFVVVSVFLTLVLLLTGINSHKHQLDFSQYKAVNIFGIQED
                     VHVADRETVTERLEQFAREHDIVIARRIVEPNETGEVFFTYDVYGQGDLPDELEVASEE
                     SAGKSDLANSYLIVSGEVDTELLRSEFDSMGFLAITSGEASPINLIMPLIAEMSTLISF
                     VIFILTFAALTIIYRVKDLRLAGIRLLAGESSWQVTLRPVKEDLILIVSSYVINLMIGL
                     VSLFALDLATMLNISLIAIGLLVLPLMYSGISVGLSIIYLMSLRRHMLVDLLKGKLPLY
                     RLISLMLVGQMIAVVVVGWSINETIVATSKYQELLSGQQIWQSNPDLVTLSAGMSADGG
                     NASESSRRDKIWYNFAHDAVENYNAMLVRNNLEQYVLNDVSDGIHKDDYVPIGNTIYVT
                     PNYLDREGIDVSPTIREKLHHLEQGEFGLLLPEKLKSESEKYIEIFTDDMNSYGKAELD
                     ADSEQLYTFQAVVDYVPNNQKRFLYNDAEIVRTQFLDDPIIVVITPKSMGETLDTFLTW
                     GADVASMLYSDYEGTIDLLKQHGVYEWVSYVEGGQLKYLQNMASMKTNLIILIAGAVLS
                     LVTSILLFVSMNILYFEQFRRELFIRRIAGMSPWENHQYYLMAQLGVITVSSGISWYLT
                     GSIGVASGTFGLFTVSALGALFIQARREHHTAMTVLKGK"
     gene            7416..8048
                     /locus_tag="FE334_RS01670"
                     /old_locus_tag="FE334_01655"
     CDS             7416..8048
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="FE334_RS01670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01655"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_172764158.1"
                     /transl_table=11
                     /translation="MLEVQNLVKAFDGRRIFDRVQVRFEPGKSYALVGASGSGKTTLLN
                     ILAYLEPFESGQVLYDGKSLTEVKSQHYFRDHMGYVFQNFGLIESQSVKDNLDLGFVGK
                     KLSKSEKISRQKRALEQVNLGYLDLKQKVFTLSGGEAQRVAIAKLILKNPPLILADEPT
                     AALDETNGKEVIQMILSLLSEERTIIIATHDPLVWEQVDEVIDVTGL"
     gene            8236..8571
                     /locus_tag="FE334_RS01675"
                     /old_locus_tag="FE334_01660"
     CDS             8236..8571
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="FE334_RS01675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FE334_01660"
                     /product="response regulator transcription factor"
                     /protein_id="WP_244325221.1"
                     /transl_table=11
                     /translation="MPVYEYQAHKLELSGFVNKNIGTEDLLSLLKHVKDGGRHFTTENW
                     FIDELTPRERELLGAIATGRKRKIIAEEMYISERTLTNHMQSIMDKLEVNSTIEAIQKA
                     RELGYLK"
ORIGIN
        1 attccattac tcggactagt tatattggcg gtgttgtcta gtcagttatt tactcaggta
       61 tggttatttt ttatggctgt tgcttcaaca cttcttatgc taattgctgg ttttaatcat
      121 aaagctgttg tgggtcaggc atcgaaatgg ttgagcttaa tattctgtac tttactttat
      181 atttatattt ttgtgcttat tttagggtta aatctaagtt tagtgctatt tatattatat
      241 attatcgtaa gtattatgta tatattgaca ttaactgacg tatttggcaa gcatcaagtg
      301 aatgctatca ggttaattaa acgggaagag gctctaaaac aagatttctc caattattta
      361 cacgatgatg ttttgcaaga tattaatgta ttgatcaaga tgacaacgtt ggagccgtct
      421 gagaagacac aagcgttctt gcgggagcaa ctgtcagctt taaatgatca attgcgccag
      481 cagatgaatc agtatagtcc acaattatcc aagcacttaa ctttgcaaga gaactatcgt
      541 ctgcttgtgc ggtcactaga gcagacatat ccgaatcaag ctgtgtcgac atctttttcg
      601 atgaatcgga atttgacttt accagaaccg tatgatgtgc tagtttatcg gtggctcagg
      661 gagttgattc ataatgtgta caagcatgca ggggcgaatc agttagatat tcatgtggca
      721 gcgtacgatg agttgataat tgtgttggtg gaagatgatg ggtgctttaa gaagggatca
      781 catcttaaga ttggtcatgg gttgtacgtg atccaagagc aagtggaagc aattggtggc
      841 caactgtata tagaggcgaa tcgtccacgg gggttgcgga tgcgggttga gttcagtgtg
      901 atgggaggcg agataattga agattttgtt aattgatgat cataagttat tttcgcaaag
      961 tttggcatta gtgctagatc agacaacaag tgatgtgcaa gtggatatga ttaatagtga
     1021 aggggagtta cctgatgatc taagcgagtt gacagtatat gatgtgttag tgttggatat
     1081 taatttagat aagggattca gtgaagatgg gttcgagttg gcagagcaga ttcaagcggt
     1141 ggtggacgtg ccgattctga tgctgacggg ctttgatttg ccggtgtatg aatatcaggc
     1201 gcacaagttg gagttaagcg gctttgtcaa taaaaatatt gataccgaag atttattgag
     1261 cctactcaag catgtgaaag atggtgggcg gcattttaca acggagaatt ggtttattga
     1321 tgagttgaca ccacgcgagc gagagttgtt aggggctatt gcgacaggca agaagcggaa
     1381 gatgatcgct gaagagcttt atatttctga gcgcacactg accaatcata tgcagagtat
     1441 tatggacaag ctagaagtga actccacgat tgaagcgatc cagaaagccc gggagttagg
     1501 ttatttgaag tgatcggtgg cgggagaaag atgacttttg gggtatacgg atcttgcgct
     1561 ttgaatgatt gcttgattta ctcatgcatt gcgctgaatt ggggagtaaa ctgggggtag
     1621 attaggtaat ggaggagatt atgatgaaat atgcaccgta tagtctgcta gccttagcag
     1681 gtatattagt ttatctgaca gtgaatcgct atattacaat gcacagcatt aatgtcgttc
     1741 ccctgattgg cgcaatcttg tgtgttattt tctttttcgt ctggcgcaat cacttgaaac
     1801 agcaagggta ttatgatgat taaatagatg aagtctcagt gaaggtgctg aggctttttt
     1861 attggagtag acttaagata atatcaagca gctgctgggg gacttcttcg agtgtgtcta
     1921 ggggaatggt ggatagctct aatagcaatg ggaagtgatc actgtcaagt gagttgggca
     1981 ggtggtgtcg ttggcaggtt ttgattaagt cacgcaggtt gatgtgatta gggagcggca
     2041 gatgtgtctt gagagcgcgc ggcggtaggt agggtaagtg ctggttaatt gggtgattgg
     2101 caacgataga gtggaacagc tctaatttcg cactgtaatg ttggtggagt tgctttaagt
     2161 ggctatccag catgcggttg tcgatgagat agagttggga caatttgttg gccggcagtg
     2221 agttatccaa aggtagataa ttagtttgtg tggtattgag atatttgatt gaagtataaa
     2281 aaaactatga ccaaagtgtt gtaaccggat tctttaagtg gtaaactgtt gttggaaaca
     2341 aattaatgaa agtagaatgt accgctgtcg aacgattaat tcaaaggggt gttaatatca
     2401 aacataaaca gttacctaag atatgttttt ggttaggagt tgttgtgttt actgtagccg
     2461 tgctgcttcc aactgattta ttccaaagca tcacctggat tgagaaaata cttggtgaat
     2521 taaaaccagt cggattagcg accatatttt tgttgccaat tatcgggagt gtaggagtgg
     2581 ttgggtccat tatgaacaaa tccgtcttgt acggcagttt gaatgttgca ttgattctgt
     2641 cattcccgct gatgatattg gtttctaata ttgtgcaggc attgttttaa atgatcaaaa
     2701 ataagttatc cataaaagaa aatattagat tttctctgaa taatgtatta cgaaggagag
     2761 ataaggatga ctattgtatc agtaagattg aacaaagaag aagagaaagc gataaatagc
     2821 tatgccacat taacgggaca gccagtatca caactgctta aacaagcact tcttagagag
     2881 attgaagaca aggtagatta tacaattgga gtagaagcgc tagaagaaca cgagaaagat
     2941 ctacagacat ttacgattga tgaagtagct ggggaactag gaattgagtt gtgagttgat
     3001 cgtattagct ctagcagttg ggtacggtaa agctatttac aagtagtacc agttacaata
     3061 aaaagacaag ctatcagaga gatgtctcag tggcttgtct ttttaatata tcaagttttg
     3121 tcagtgagca actgactcat taatagctga acttcttggg cgccaatagt atcgccacct
     3181 tgttcaaata attgaatcgc tcgcttgaaa tatttccttg caagagacgg cttgtgttgg
     3241 gcgacgcata tttcacccat accacgctga gcgcatgctt gagcaattaa atcatctgtt
     3301 tgttctgctt gtgttaaact ttgagccata aaagagactg cttcgtttag ttgttgcaat
     3361 ttcattctta agtagccttg ttcgtagtta ttgacggact gtttaagtaa gttattagga
     3421 aagtgctgtt caataatacg tgcttcttgt tgaatcaatt ccaatgcttg tccgtaatct
     3481 tcacgcatgc gatagaccat cgccagttga tgtaatgcta tatgttcttg ttctatatca
     3541 tgatttttac ggcttaattg tagccactgt tggtaagtag attcagcttt atctatttta
     3601 ccgtcttcta gatagatata gccgagtaag ttcaatgcat tgtaatcagt cgttgcttgt
     3661 tcatttatat aatcatcaac gagttgtttg gcttttggga tatttccgcg cgaaaattgt
     3721 ccccatgctt gatttatcat ttatttcact ccatttgtct attgatttat tatactggat
     3781 tcagaataat atttcgagta tttattaaaa ataattattt taatcataac tatattattt
     3841 tataataaaa ttaataaaaa tattggagga attatgatga ttggaccaaa aatgaaagca
     3901 cgtcggaaaa cattgggatt gaatcaacgt gagttagcag aagggatatg tgcacaggcg
     3961 ttaattagta agattgagcg agaatcgtcg tcaccaagta gtaaaatact acaaaaactt
     4021 agtgatcgat taggcgtgcc ggtgacgtat ttcttaagtg attcagatga tggtgatatt
     4081 gaagcggcta aaaataaaga aaattataag cgactagaaa ctttattacg cgctgagtta
     4141 atgaaaggtg agtatcagac aatctcaatg atgatggcga ttaatgcaga tttaattgct
     4201 gcgtgtcgtg atgactactt tattaatcta ttcaagtggc ttcaaggtgt attatattac
     4261 cgaattgacc gtaacccgga taaagctctt gggttactca aagaaatcga aagtcaagtc
     4321 gctgatgaat atattgagtt acacattgat attttgatga gtattgggat gatttatgat
     4381 gaattaagcg atgatgaacg ggcatcagat gcctatcagg aagcaacgtt cttactgaat
     4441 caatctattt catttaaaaa acgggttcat gtcctgcgta atcatgcact aagtttgaaa
     4501 aatatggatg aaatgaaaga agcgttgaag tatgtattga gagccatcga agtgcttatt
     4561 aaacacaaat cactgcatat gatgggagat ttatatcttt taaaaggtaa cttattcgag
     4621 cgattaggcg atactgaaga agccacccaa acttatgaag atgcaatttg ggtgtttaag
     4681 acactgaata tcaaggaatc attgacagta gcaactgttg cattgcataa tattttacct
     4741 aataataaat taaacaatta aattattaat ataaaaaaca accaaaaaac atttttgaaa
     4801 ttttgataaa aaaagtgttg atattgtatt taggtgggta tatacttatc tcatacctaa
     4861 gatatccagt tatcttaaga cttgtcaatg agaagcgata aatttatgaa aggttgcaaa
     4921 aagagtggtc gcattatcta ttaggagcca ttaagctaac caatattcat aggtaaactc
     4981 atataaagga ggttttcaca atgaaaacca caaacaaact atcaaaaaca ttattagcag
     5041 ttgcagcgat tgcggcattg gcagctccag tatcagcagc ttctcgattt gtttcaggag
     5101 gagtatggac gtatggagca ggaggtggtg gagcattctc gaattattat catggacaac
     5161 gacgacactc ttcaaccgtt gtgagcttat caaattctaa aagcaaaaaa gcatgggcta
     5221 aaaaggaaca aacatcaaag gcatttattc ctacaaaatt tggtgaaaaa gcagcctttt
     5281 actacggtgt tgactagata gaatcaatat ctttttacaa tctgattact tttaaaataa
     5341 caaagtaatc agattgtatt tatagataga tggaaagaag aatgagcaat gataaagaaa
     5401 atatttgtcg tagtatctgt ttttttgact ttagtattgt tattaacagg aataaattca
     5461 cataagcatc aactggattt ctcacaatat aaggcagtta atatatttgg tattcaagaa
     5521 gatgtacatg tagctgatcg tgaaactgta actgaacgat tagaacaatt tgctagggag
     5581 catgatattg tgattgctag acgtattgtt gaaccgaatg aaactggtga agtatttttt
     5641 acgtatgatg tgtatggtca aggtgacctc cccgatgaat tagaagtagc ttcagaagaa
     5701 agtgcaggaa aaagtgacct ggccaatagt tatttaattg tctcaggtga agttgatacg
     5761 gagctgttga gaagtgaatt tgatagtatg ggattcctag cgattactag tggagaagct
     5821 tcgcctataa atcttattat gcctttaata gcagaaatgt caacgttaat tagttttgtt
     5881 atatttatat taacctttgc cgctttaacc atcatctatc gagtaaaaga tttaaggtta
     5941 gcaggcattc gattgttagc aggagaatct tcatggcaag taacgctacg accagtgaaa
     6001 gaagatttga tcctaattgt atcatcatat gtgattaatt tgatgattgg attagtttct
     6061 ttattcgctc ttgatcttgc aactatgctt aatatcagtc ttatagcaat aggtctgctg
     6121 gttcttcccc taatgtattc agggatatca gtgggcttga gtataattta tttaatgagt
     6181 ttgagacgac atatgttagt tgatctactt aaagggaaac ttccattgta ccgtttaatt
     6241 agcttaatgt tagttggtca gatgatagca gtcgttgtcg ttggatggag tattaatgaa
     6301 acgattgttg ctactagcaa gtatcaagaa ttattatctg gtcaacagat ttggcaaagc
     6361 aatcctgatt tagtgacttt atcagctgga atgtcagcag atggggggaa tgcctccgaa
     6421 agtagccgac gtgataaaat ttggtataat tttgctcatg atgcagtaga aaattataat
     6481 gctatgctag ttagaaataa cttagagcaa tatgtattga atgatgtgtc agatggtatt
     6541 cataaagatg actatgttcc tataggtaat acaatttatg taacacctaa ttacttagat
     6601 agagaaggaa ttgatgtatc tcctactatt cgtgaaaaat tacatcattt agaacagggg
     6661 gaatttggct tgctccttcc tgaaaaatta aaatcagaaa gtgaaaaata cattgagata
     6721 tttacagatg atatgaatag ctatggtaaa gcagaattag atgcagatag cgaacaactt
     6781 tatacttttc aagctgttgt cgattatgta cctaacaatc agaaacgttt cttatataat
     6841 gatgcggaga tagttagaac acaattttta gatgacccga ttattgtcgt tataactccc
     6901 aaatcaatgg gagaaactct agatacattc ttgacttggg gggcagatgt ggccagtatg
     6961 ctttactctg attacgaggg aacaattgat ttacttaaac aacacggtgt ttatgagtgg
     7021 gtttcgtatg tagaaggcgg acagttgaag taccttcaga atatggcgag tatgaagact
     7081 aacttaatca ttctcattgc cggcgctgtc ttaagtttag tgacatcaat tctactgttt
     7141 gtgtcaatga acatattgta tttcgagcag ttccgtcgtg agttattcat tagacgcatt
     7201 gctggcatga gcccatggga aaatcatcaa tactacctga tggctcaact cggagtcatt
     7261 acggtttcat caggcatctc ttggtattta accggcagta tcggggtggc ttcaggtaca
     7321 ttcggcttat tcactgtcag tgcattgggt gccttattca tccaagcacg gcgggaacac
     7381 cacacggcaa tgaccgtact gaaaggaaaa taatgatgtt agaagttcag aatttagtaa
     7441 aagcctttga tgggcgacgg atttttgacc gtgttcaggt gagatttgag ccggggaaat
     7501 cgtatgcctt agttggggcg agtggcagcg ggaagacgac tttgttaaat atcttagcgt
     7561 atttagagcc gttcgagagt gggcaggtgc tgtatgatgg caagtctctg acggaggtga
     7621 agtcgcagca ttatttccgc gatcatatgg gatatgtatt tcagaatttt gggttaattg
     7681 agagtcagtc ggtgaaggat aatttggact taggatttgt cgggaagaaa ttatcgaaat
     7741 cagagaaaat atcgaggcaa aaacgagcgc tagaacaagt taatttgggc tatttggatc
     7801 tgaagcaaaa agtgttcaca ttatctggcg gggaagcgca acgggtggcg attgcgaaat
     7861 taatcttgaa gaatccgcca ttgatacttg ctgatgagcc cactgccgca cttgatgaga
     7921 caaatggcaa agaagtgatt cagatgattt tatcgctgct gagcgaagag cgaactatta
     7981 ttatcgccac acacgatcca cttgtctggg agcaagtcga tgaagtcatt gatgtgacgg
     8041 ggttataaga ctgtttaaga gtcaagttta cggcgggcag accgtgaaac ttggcttttt
     8101 tcagggtgaa tattattgtc ttatacataa ttcaggggag gaaatttcga atcgctaacc
     8161 ccttgatatg atagggattt caccgatcca aaaataaaca tcgacagacc aaaccctttc
     8221 ataataggat ttgatttgcc ggtctatgaa tatcaggcgc ataaattgga attaagtgga
     8281 tttgtcaata aaaatattgg taccgaagat ttattgagtc tactcaagca tgtgaaagat
     8341 ggtgggcggc attttacaac ggagaattgg tttattgatg agttgacacc acgcgaacga
     8401 gagttgttag gggctatcgc gactgggagg aagaggaaaa taattgctga agagatgtat
     8461 atttccgagc gcacgctgac caatcatatg cagagcatta tggataagct agaagtgaac
     8521 tccacgattg aagcgatcca gaaagcccgg gagttaggtt atttgaagtg ataaaagata
     8581 ttatgaataa atagataaag tctcagaatt gtgtactgac ccccaaaaaa atgttagagt
     8641 aaatcattct aacttttggg ggtcacatca ttttcaggga ttgttttagt cgtattgtga
     8701 tgcggtcata cactagatag ggctacaagc tcatccctcg catgtattca atttcttctt
     8761 aagttgtttt atcttttgaa aagattatag accaataaaa agtcaaattc tacggcgggc
     8821 agacggtgga atttgacttt ttaaatgagc taataaaatt gagtcaatca ctagggttta
     8881 tacgagcgtg ttcttttggt aaaataggaa ttgacaatag ggaagatgag ataatatagg
     8941 ggagttaatt gatgtcaaag aaaaaattgt tattaattga tggatatagt attgcgttcc
     9001 gggcattcta tgcgatgcat agtcagttgc accggatgaa gaataagaat gggctacata
     9061 cgaatgcact ctatgggttc cataatatgt tagaagcagt gatggaaaaa gaacaaccga
     9121 cgcatgcatt agtggccttc gatgcgggga agactacttt tcggcatgag ttgtttgatg
     9181 attataaggg cggtcgtgag agtatgccgg ccgaattgtc tgaacagata ccttatttga
     9241 aggaattaat tgcagcgtat ggtctacaga cgtatcaatt gccggattat gaagcggatg
     9301 atattatcgg aaccttaagt actcaagcat cagatgaatt cgaagtcgtc attattacgg
     9361 gggacaagga tttgacgcaa ctggccagtg atcagacccg ggttgatatt acgaagaagg
     9421 ggattacgca gttgaagtcg tatacgccgg aatcaatgat ggaagagatg gggattaccc
     9481 cagaacaaat taccgatctg aagggattat cgggggattc ttcggataat attccaggtg
     9541 tgacacagat tggagataag acggcactta agttgttgaa gaagttccat acagtagaag
     9601 aattgtatga acgtattgat gagatgaatg cttctaagcg caaggaatat ttaatcgctg
     9661 agaaagaaac agcgctactc agcaaacagt tggcgacgat tattactgat gcgcctattg
     9721 agatttcacc ggagtcatta gcttattcgg ggaaaaatat ggaggcgttg attggcttct
     9781 ataaggaaat ggacttcaat agtcatttag aaaagttaga tacggatgtg tacttcgaag
     9841 aattacaaga tgaagcagaa gcggtcgaat atgaatgggt tgaagaagta acgaaagcaa
     9901 tgttcggaca gagcggaaca ttgtattttg aaatgttcga agagaattac catacggcac
     9961 cttatattat tgcgagctgg actaatgaga agaaagtcta tgtagctgag ccagagacgt
    10021 tattagcttc caaggctttc caagattggc tagaagatga gtcagcccgg aagtatacgt
    10081 acgatgcgaa agcattaatg gtatccttga agcaacgtga attggagatt gctaatatta
    10141 cttttgattt atcgttggca tcgtatgtat tgaccgctga gaattctagt ggaggcgatg
    10201 tggcggctat tgccaacaaa catggatatg tggatgtctt agaagatgaa gcggtctacg
    10261 gtaaagggaa aaaagtaagt attcctgatg ataaaac
//
