LOCUS       NZ_JAGFWR010000048      7478 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Micromonospora antibiotica strain MMS20-R2-23 contig48, whole genome
            shotgun sequence.
ACCESSION   NZ_JAGFWR010000048
VERSION     NZ_JAGFWR010000048.1
KEYWORDS    WGS; RefSeq.
SOURCE      Micromonospora antibiotica
  ORGANISM  Micromonospora antibiotica
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Lee,D.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2021) Microbiology & Molecular Biology, Chungnam
            National University, 99, Daehak-ro, Yuseong-gu, Daejeon 34134, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.63x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017599305.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 02:05:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,561
            CDSs (total)                      :: 6,498
            Genes (coding)                    :: 6,378
            CDSs (with protein)               :: 6,378
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 20 of 120
            Pseudo Genes (incomplete)         :: 107 of 120
            Pseudo Genes (internal stop)      :: 2 of 120
            Pseudo Genes (multiple problems)  :: 9 of 120
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:14:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7478
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAGFWR010000048.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2292
                     /locus_tag="JQN83_RS32400"
                     /old_locus_tag="JQN83_32395"
     CDS             <1..2292
                     /NRPS_PKS="Domain: PKS_DH (78-232). E-value: 1.3e-43.
                     Score: 141.2. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32400_PKS_DH.1"
                     /NRPS_PKS="Domain: ACP (687-760). E-value: 8.2e-27. Score:
                     85.3. Matches aSDomain: nrpspksdomains_JQN83_RS32400_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF044701.2"
                     /locus_tag="JQN83_RS32400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32395"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_208570963.1"
                     /sec_met_domain="PP-binding (E-value: 1.1e-13, bitscore:
                     39.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SLGVVGSGGVVVPTLRRGEGGVGRVLLSLGEAFVAGVEVDWGRVV
                     PEAVRVDLPTYPFQHEHYWLKTTPSGVTTDRGDHALLGAPVELPGSGGVVLTGTVSLAG
                     LPWLAAHTVGDTVVLPGTALLDLALKAGDDVGLDHVDALTLHEPLTVPARAAVHLRVEI
                     GAPGDDARRPLSIHARRADTPAGAPWRCHASGTLAVGVPSPGVDLTVWPPPAATGEPAA
                     DGVARWRRDGEVFAEVRLPDRQRAEADQFGLHPVLLDAALRAVDDPADAPSRLAELRGV
                     TLRAVGATVLRVHRAASGQVTVADATGSPVATIAAVTTRPCPTGEVRTASAAAQDGLFR
                     VEWVPVSTPATPPSPPTMVVVGADPLRARAGLMSADTYAEAYPDLAALTAAVEAGAARP
                     DVILLSDTDPAPSAGSPWETSRLVTLRPDPTVAAPRRRGRAGTTGTAAVPATAVPATAV
                     PATAVPATAVPATAVPADAVPADAVPAGATPAHGDTVVVDGVKASWRALMTALALAEPH
                     LVVRRGTVLTPRLARVTVPVDDATPPDVTGPVLVTGGASAARGATVAFLRGCGIEAGDG
                     SGDAATTVVRLDHPDATATSATPTVALLRERPAGDLSPDELVLVEAACRVGGSLVVAAG
                     AGAWSRSPDAPALLRSLAPAGTRRTARAVADAPAGLKQRLVLAPEGDRDQILLELVRRN
                     VAAVLGLASAETVERDRRFRDAGFDSLSALTVRNLISTETDLRLAPTVVFDHSTPDALV
                     RHLRQELLGY"
     source          1..7478
                     /collection_date="2018-03-14"
                     /db_xref="taxon:2807623"
                     /geo_loc_name="South Korea: Sejong-si"
                     /isolation_source="riverside soil"
                     /mol_type="genomic DNA"
                     /organism="Micromonospora antibiotica"
                     /strain="MMS20-R2-23"
                     /submitter_seqid="contig48"
                     /type_material="type strain of Micromonospora antibiotica"
     region          1..7478
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="PKS-like"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="(t2pks2 or ksIII)"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..7478
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2pks2 or ksIII)"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="neighbouring"
                     /product="PKS-like"
                     /product="T1PKS"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..7478
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2340:3378](+)"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2341..3378
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
     protocluster    1..7478
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4359:>7478](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      4360..>7478
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="2"
     CDS_motif       235..288
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32400_0001"
                     /evalue="3.60E+00"
                     /label="PKSI-DH_m2"
                     /locus_tag="JQN83_RS32400"
                     /protein_end="95"
                     /protein_start="77"
                     /score="0.5"
                     /tool="antismash"
                     /translation="DHALLGAPVELPGSGGVV"
     aSDomain        238..699
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32400_PKS_DH.1"
                     /evalue="1.30E-43"
                     /label="JQN83_RS32400_PKS_DH.1"
                     /locus_tag="JQN83_RS32400"
                     /protein_end="232"
                     /protein_start="78"
                     /score="141.2"
                     /tool="antismash"
                     /translation="HALLGAPVELPGSGGVVLTGTVSLAGLPWLAAHTVGDTVVLPGTA
                     LLDLALKAGDDVGLDHVDALTLHEPLTVPARAAVHLRVEIGAPGDDARRPLSIHARRAD
                     TPAGAPWRCHASGTLAVGVPSPGVDLTVWPPPAATGEPAADGVARWRRDG"
     aSModule        238..2283
                     /domains="nrpspksdomains_JQN83_RS32400_PKS_DH.1"
                     /domains="nrpspksdomains_JQN83_RS32400_ACP.1"
                     /incomplete
                     /locus_tags="JQN83_RS32400"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       322..399
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32400_0002"
                     /evalue="4.80E-08"
                     /label="PKSI-DH_m2"
                     /locus_tag="JQN83_RS32400"
                     /protein_end="132"
                     /protein_start="106"
                     /score="25.5"
                     /tool="antismash"
                     /translation="WLAAHTVGDTVVLPGTALLDLALKAG"
     CDS_motif       1333..1404
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32400_0003"
                     /evalue="2.90E+01"
                     /label="PKSI-DH_m2"
                     /locus_tag="JQN83_RS32400"
                     /protein_end="467"
                     /protein_start="443"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="GTAAVPATAVPATAVPATAVPATA"
     aSDomain        2065..2283
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32400_ACP.1"
                     /evalue="8.20E-27"
                     /label="JQN83_RS32400_ACP.1"
                     /locus_tag="JQN83_RS32400"
                     /protein_end="760"
                     /protein_start="687"
                     /score="85.3"
                     /tool="antismash"
                     /translation="LELVRRNVAAVLGLASAETVERDRRFRDAGFDSLSALTVRNLIST
                     ETDLRLAPTVVFDHSTPDALVRHLRQEL"
     gene            2341..3378
                     /locus_tag="JQN83_RS32405"
                     /old_locus_tag="JQN83_32400"
     CDS             2341..3378
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: ksIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF020130.5"
                     /locus_tag="JQN83_RS32405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32400"
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="WP_208570964.1"
                     /sec_met_domain="ksIII (E-value: 8.4e-78, bitscore: 249.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVEGVYLDGIGHHLPDWVSAEDAVADGRYRAEVFEASGLTGTHI
                     STKLPALDMAVLAARTALERSSAGTADVESHIHASVYYQGPEGSYPPGYILRELGVAGA
                     GSLEIRQGCNGLLAALEIAVGQLTGVAELAVALLTTAQNFETPLVDRWHGFGDSYILAD
                     GAAAAVVTTESGFAEVRSICSGTLPGLERWHRGDGPLTPPVEQSAEQVSMTARAAAFTD
                     DEMPLARAVESLSEFDVGIIRRALVDADLNVADLARVITINEDARMTEFTTLGPLGIDV
                     SLSTWDHGRSVGHVGAADLVISLDHLLDTGQVHPGDHLLLVSQGPGWLCSAAVLTLLEK
                     PSWLE"
     misc_feature    2364..2378
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(3388..3401)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            3460..4317
                     /locus_tag="JQN83_RS32410"
                     /old_locus_tag="JQN83_32405"
     CDS             3460..4317
                     /NRPS_PKS="Domain: Abhydrolase_1 (29-134). E-value:
                     1.9e-16. Score: 52.3. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32410_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.5"
                     /locus_tag="JQN83_RS32410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32405"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_208570965.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-17, bitscore: 51.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.4e-12, bitscore:
                     35.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDAIVADDVDAGTFGVPGARLYYEVQGQGPVLLVLQAGEGDAGRT
                     SYLVQQLADRYTVVTYDRRGLSRSKLDGPPAALTMETHADDASALLAHLTDQPAHVLGS
                     GYGAAIGLYLSLRHPEQVRTLVAHEAPTLRLLPAATRERVVRGVAAAERAYHNDGWGPA
                     MKQMAALSGIDLTTLEMEPGVDFQPMTVDRAANIEFFVKYDGPAFRNCQLDADDIARLK
                     QTGIRVVPAGGCISQDIWAYVCAEALADELGTAVVEFPGGRNGHTTHPRAFSARLHEVL
                     AAVA"
     misc_feature    complement(3473..3487)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     aSDomain        3547..3861
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32410_Abhydrolase_1.1"
                     /evalue="1.90E-16"
                     /label="JQN83_RS32410_Abhydrolase_1.1"
                     /locus_tag="JQN83_RS32410"
                     /protein_end="134"
                     /protein_start="29"
                     /score="52.3"
                     /tool="antismash"
                     /translation="PVLLVLQAGEGDAGRTSYLVQQLADRYTVVTYDRRGLSRSKLDGP
                     PAALTMETHADDASALLAHLTDQPAHVLGSGYGAAIGLYLSLRHPEQVRTLVAHEAPTL
                     R"
     gene            4360..>7478
                     /locus_tag="JQN83_RS32415"
                     /old_locus_tag="JQN83_32410"
     CDS             4360..>7478
                     /NRPS_PKS="Domain: PKS_KS(Hybrid-KS) (11-418). E-value:
                     5.6e-163. Score: 534.7. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32415_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (539-836). E-value: 3.3e-104.
                     Score: 340.4. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32415_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_PP (931-1013). E-value: 3.2e-18.
                     Score: 57.8. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32415_PKS_PP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183261.1"
                     /locus_tag="JQN83_RS32415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32410"
                     /product="type I polyketide synthase"
                     /protein_id="WP_208570966.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.9e-82, bitscore:
                     263.7, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_KS (E-value: 3.7e-164, bitscore:
                     534.7, seeds: 2284, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.2e-105, bitscore:
                     340.4, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.5e-11, bitscore:
                     31.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSTAPAYQDAIAVIGMSCRFPGAASPDEFWDLLVRGEEAIREAP
                     ADRWSGDPGTVRRGGFLDDVAGFDAPFFGVSPREAAAMDPQQRLMLELAWEALEHAGAD
                     PTPLRGSSTGVFIGTIQDDYATLTRRGGGDPGPHTLTGLHRSVIANRVSYRLGLAGPSM
                     VVDTGQSSALVAVHRAIESLHAGECTMVLAGGVNLILTGESTDAVRGFGGLSPTGRTHL
                     FDAHADGYVRGEGGGLVVLKRLADAVADGDRIFCVIHGGAVNNDGGGDGLTTPSQAGQE
                     QVVQLAHTRAGTDPADTGYVELHGTGTPVGDPIEAAALGAVVGAALAPGDRLLVGSAKT
                     NVGHLEGAAGVVGLIKTALALRHGLIPASLHHETPNPAIDFAATRLTVQTTLAGWPVTR
                     PLAGVSSFGMGGTNCHLVLGPWPAPPAAVDAPTSVDGPGTPSGTPVAAPAVARVVWPLS
                     GRTPAALRAQAHRLHHHLTDRPTVPIGDVGRSLAGRSGFDHRAVVVGRDRAELLAGLAD
                     LADGRRRAGLVTGRADTGTLGFLFTGQGAQRPGMGRDLYDASPVFADAFDEVCAELDRH
                     LDRPLREVVWDDPTALDQTGYTQPALFALQTALHQLTVSFGVRPDYLLGHSIGELSAGY
                     AARVWSLPDACLLVAARGRLMQAAPAGGAMLAIAAPEDVVTAHLADFAARLDLAAVNGP
                     NAVVVSGDADAVTDLAARFDQLAVKTRRLRVSHAFHSAHMDPALDEFTRVAAGIPARTP
                     QVPVVSNVSGKLVQAEYTDPTYWAAQIRRPVRFLDGLRTLRSAGVTAFLELGPDPVLTG
                     LAEAEQDAGPGSSDDTAYAVLLRRDRDEPTTALTALARLHSHGVPVNWSPLWPDADRAD
                     LPTYAFQRRPYWIDTAASSAAAASSAGSAVPVDAPSSTAPEEPATGAAEVDPASWAGRL
                     LALPEHERRPAAELLVRRLVAETLDYADLADVDGSLTFKELGITSLTAVELRNLVTAET
                     GQSFSASMVFDFPTPVLVAERLVGLVVGLGSGVGVAGVGVGEAVAIVGMG"
     aSDomain        4393..5613
                     /ASF="found active site cysteine: False, scaffold matched
                     GSSS: False"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32415_PKS_KS.1"
                     /domain_subtypes="Hybrid-KS"
                     /evalue="5.60E-163"
                     /label="JQN83_RS32415_PKS_KS.1"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="418"
                     /protein_start="11"
                     /score="534.7"
                     /tool="antismash"
                     /translation="IAVIGMSCRFPGAASPDEFWDLLVRGEEAIREAPADRWSGDPGTV
                     RRGGFLDDVAGFDAPFFGVSPREAAAMDPQQRLMLELAWEALEHAGADPTPLRGSSTGV
                     FIGTIQDDYATLTRRGGGDPGPHTLTGLHRSVIANRVSYRLGLAGPSMVVDTGQSSALV
                     AVHRAIESLHAGECTMVLAGGVNLILTGESTDAVRGFGGLSPTGRTHLFDAHADGYVRG
                     EGGGLVVLKRLADAVADGDRIFCVIHGGAVNNDGGGDGLTTPSQAGQEQVVQLAHTRAG
                     TDPADTGYVELHGTGTPVGDPIEAAALGAVVGAALAPGDRLLVGSAKTNVGHLEGAAGV
                     VGLIKTALALRHGLIPASLHHETPNPAIDFAATRLTVQTTLAGWPVTRPLAGVSSFGMG
                     GTNCHLVL"
     aSModule        4393..7398
                     /complete
                     /domains="nrpspksdomains_JQN83_RS32415_PKS_KS.1"
                     /domains="nrpspksdomains_JQN83_RS32415_PKS_AT.1"
                     /domains="nrpspksdomains_JQN83_RS32415_PKS_PP.1"
                     /locus_tags="JQN83_RS32415"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       4837..4884
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0001"
                     /evalue="9.40E-04"
                     /label="PKSI-KS_m3"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="175"
                     /protein_start="159"
                     /score="11.8"
                     /tool="antismash"
                     /translation="GPSMVVDTGQSSALVA"
     CDS_motif       5026..5064
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0002"
                     /evalue="6.70E-05"
                     /label="PKSI-KS_m4"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="235"
                     /protein_start="222"
                     /score="15.7"
                     /tool="antismash"
                     /translation="FDAHADGYVRGEG"
     CDS_motif       5257..5304
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0003"
                     /evalue="1.80E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="315"
                     /protein_start="299"
                     /score="13.5"
                     /tool="antismash"
                     /translation="YVELHGTGTPVGDPIE"
     CDS_motif       5365..5409
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0004"
                     /evalue="2.10E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="350"
                     /protein_start="335"
                     /score="10.8"
                     /tool="antismash"
                     /translation="GSAKTNVGHLEGAAG"
     aSDomain        5977..6867
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32415_PKS_AT.1"
                     /evalue="3.30E-104"
                     /label="JQN83_RS32415_PKS_AT.1"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="836"
                     /protein_start="539"
                     /score="340.4"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGAQRPGMGRDLYDASPVFADAFDEVCAELDRHLDRPLREV
                     VWDDPTALDQTGYTQPALFALQTALHQLTVSFGVRPDYLLGHSIGELSAGYAARVWSLP
                     DACLLVAARGRLMQAAPAGGAMLAIAAPEDVVTAHLADFAARLDLAAVNGPNAVVVSGD
                     ADAVTDLAARFDQLAVKTRRLRVSHAFHSAHMDPALDEFTRVAAGIPARTPQVPVVSNV
                     SGKLVQAEYTDPTYWAAQIRRPVRFLDGLRTLRSAGVTAFLELGPDPVLTGLAEAEQDA
                     GPGSSDDTAYAVLLRR"
     CDS_motif       5980..6027
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0005"
                     /evalue="2.90E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="556"
                     /protein_start="540"
                     /score="19.6"
                     /tool="antismash"
                     /translation="FTGQGAQRPGMGRDLY"
     CDS_motif       6208..6330
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0006"
                     /evalue="5.90E-21"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="657"
                     /protein_start="616"
                     /score="66.7"
                     /tool="antismash"
                     /translation="GVRPDYLLGHSIGELSAGYAARVWSLPDACLLVAARGRLMQ"
     CDS_motif       6424..6462
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0007"
                     /evalue="1.20E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="701"
                     /protein_start="688"
                     /score="12.2"
                     /tool="antismash"
                     /translation="AAVNGPNAVVVSG"
     CDS_motif       6682..6717
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0008"
                     /evalue="2.30E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="786"
                     /protein_start="774"
                     /score="14.6"
                     /tool="antismash"
                     /translation="YWAAQIRRPVRF"
     CDS_motif       7006..7041
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32415_0009"
                     /evalue="6.80E-01"
                     /label="PKSI-KS_m3"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="894"
                     /protein_start="882"
                     /score="3.4"
                     /tool="antismash"
                     /translation="WIDTAASSAAAA"
     aSDomain        7153..7398
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32415_PKS_PP.1"
                     /evalue="3.20E-18"
                     /label="JQN83_RS32415_PKS_PP.1"
                     /locus_tag="JQN83_RS32415"
                     /protein_end="1013"
                     /protein_start="931"
                     /score="57.8"
                     /tool="antismash"
                     /translation="ALPEHERRPAAELLVRRLVAETLDYADLADVDGSLTFKELGITSL
                     TAVELRNLVTAETGQSFSASMVFDFPTPVLVAERLVG"
ORIGIN
        1 tcgttgggtg tggtgggttc gggtggtgtg gtggtgccga cgttgcgtcg gggtgagggt
       61 ggtgtgggtc gggtgttgtt gtcgttgggt gaggcgtttg ttgcgggggt ggaggttgat
      121 tggggtcggg tcgttccgga ggctgtccgg gtcgacctgc ccacctaccc cttccagcac
      181 gagcactact ggctcaagac caccccgtcc ggcgtcacca ccgatcgtgg cgaccatgcg
      241 ctgctcggcg ccccggtgga gctgcccggc tccggcgggg tggtgctgac cggcaccgtc
      301 tccctggccg gcctgccctg gctggccgcc cacaccgtcg gcgacaccgt cgtactgccc
      361 ggcaccgccc tgctcgacct ggcgctgaag gccggcgacg acgtcgggct cgaccacgtc
      421 gacgccctga ccctgcacga gccgttgacc gtgccggccc gggcggctgt ccacctgcgg
      481 gtggagatcg gcgcaccggg ggacgacgcc cgccggccgc tgagcatcca cgcccgacgg
      541 gcggacaccc cggccggagc gccctggcgc tgccacgcct ccggcacgct cgccgtcggc
      601 gtaccgtcgc cgggtgtcga cctgacggtg tggccgccgc cggccgcgac cggagagccc
      661 gccgccgacg gtgtcgcgcg ctggcggcgc gacggtgagg tcttcgccga ggtccggctg
      721 cccgaccggc agcgcgccga ggcggaccaa ttcggactgc acccggtcct gctggacgcc
      781 gcactgcggg ccgtcgacga cccggccgac gcgccgtccc ggctggccga gttgcgcggc
      841 gtgaccctgc gggcggtggg cgcgaccgtg ctgcgggtgc accgtgccgc gtcgggtcag
      901 gtcaccgtcg ccgacgcgac cggttccccg gtggcgacga tcgccgccgt caccacccgc
      961 ccctgcccga ccggtgaggt ccggaccgcg agcgccgccg cccaggacgg cctgttccgg
     1021 gtggagtggg tcccggtgtc cacgccggcc accccgccgt cgccgccgac gatggtcgtc
     1081 gtcggtgccg atcccctgcg ggcccgcgcc gggctgatgt cggcggacac ctacgccgag
     1141 gcgtaccccg acctggccgc gctcaccgcc gcggtcgaag ccggcgcggc ccgcccggac
     1201 gtcatcctgc tcagcgacac cgaccccgcg ccgtcggccg gcagcccctg ggagaccagt
     1261 cgcctcgtca ccctgcgccc ggacccgacc gtcgcggccc cccgccgccg tggccgggcg
     1321 ggcaccaccg gaaccgccgc ggtaccggcc accgccgtac cggccaccgc cgtgccggcc
     1381 accgccgtgc cggccaccgc cgtgccggcc accgccgtgc cggccgacgc ggtgccggcc
     1441 gacgcggtgc cggccggtgc gacgcccgcc cacggcgaca ccgtcgtggt ggacggcgtc
     1501 aaggcgtcct ggcgggcact gatgacggcg ctcgccctgg ccgaaccgca cctggtcgtc
     1561 cgtcgcggga cggtgctgac gccccgcctg gcccgggtca ccgtcccggt ggacgacgcg
     1621 acgccgcccg acgtcaccgg gccggtgctg gtcaccggcg gcgcgtcggc ggcgcgcggg
     1681 gcgaccgtgg ccttcctgcg cgggtgcggg atcgaggccg gcgacggcag cggggacgca
     1741 gccaccaccg tggtccggct ggaccacccc gacgcgacgg ccaccagcgc caccccgacc
     1801 gtcgcgctgc tgcgggaacg gcctgccggg gacctgtcac ccgacgagct cgttctcgtc
     1861 gaggcggcct gccgtgtcgg cgggtcactg gtcgtcgcgg ccggggccgg cgcctggtcg
     1921 cgctcccccg acgccccggc gctgctgcgg tcgttggctc cggccggcac ccgccgcacc
     1981 gcacgggccg tcgccgacgc cccggccggc ctcaagcagc ggctggtgct cgcgcccgag
     2041 ggcgaccggg accagatcct gctggagctg gtacgccgca acgtcgcggc ggtactcggc
     2101 ctggcgtcgg ccgaaaccgt cgaacgggac cggcggttcc gggacgccgg gttcgactcg
     2161 ctctccgccc tgacggtccg caacctgatc agcacggaga ccgacctgcg gctcgcgccc
     2221 acggtggtct tcgaccactc cacgcccgac gccctggtgc gtcacctcag gcaggaactg
     2281 ctcggctact gacgatccgc cgggctgcgg cggaacacag ctgaagaatg gagggacccc
     2341 gtgcgagtcg aaggtgtcta tctcgacggg atcggccacc acctacccga ctgggtgagc
     2401 gccgaggacg cggtcgcgga cggccggtac cgggcggagg tcttcgaggc gagcggcctg
     2461 accggcacgc acatctcgac gaaactgccc gccctcgaca tggcggtgct ggccgcccgg
     2521 accgcgctgg aacggtcctc ggccggaacc gccgacgtcg agtcgcacat ccacgcgagc
     2581 gtctactacc aggggcccga gggctcctac ccacccggct acatcctgcg tgaactggga
     2641 gtcgccggtg ccggctccct ggagatccgg caggggtgca acgggctgct cgccgcgctg
     2701 gagatcgccg tcgggcagct caccggcgtg gccgaactcg ccgtggctct gctgaccacc
     2761 gcccagaact tcgagacacc gctggtcgac aggtggcacg gcttcggcga ctcgtacatc
     2821 ctcgccgacg gtgcggcggc ggccgtcgtc accaccgaat ccggcttcgc cgaggtccgg
     2881 tcgatctgtt ccggcaccct gcccgggctg gaacggtggc accgcggcga cggcccgttg
     2941 accccgccgg tcgagcagag cgccgaacag gtgtcgatga ccgcgcgggc ggccgccttc
     3001 accgacgacg agatgccgct ggcccgggcc gtggagagcc tcagcgagtt cgacgtgggc
     3061 atcatccgcc gggcgctggt cgacgcggac ctcaacgtcg ccgacctggc ccgggtgatc
     3121 accatcaacg aggacgcccg gatgaccgag ttcaccaccc tcggcccgct cggcatcgac
     3181 gtgtctctat caacttggga ccatggccgg tcggtcggcc acgtcggcgc cgccgacctg
     3241 gtcatttcac tggaccacct gctcgacacc gggcaggtcc accccggcga ccacctcctg
     3301 ctggtctccc agggcccggg atggctctgc tccgccgccg tcctcaccct gctggagaag
     3361 ccgtcctggc tggagtgaag tgccgtttta gatgcgttct aggccctctg tctagcgtcg
     3421 aaccgtcaca cgttccgagt gcgcaacgga ggggattcag tggacgcgat cgtggccgac
     3481 gatgtcgatg ccgggacctt tggcgtaccc ggagcacgtc tgtattacga ggtccagggc
     3541 caggggccgg tattgctggt cctccaggcc ggtgagggtg acgccggccg gaccagctat
     3601 ctcgtacaac aactcgccga ccggtacacg gtggtgacct acgaccggcg cggcctgtcc
     3661 cgcagcaaac tcgacgggcc acccgcagcg ctgaccatgg agacccacgc cgacgacgcg
     3721 agcgccctgc tcgcccacct caccgaccag ccggcccacg tgctcggctc cggctacggt
     3781 gcggcgatcg ggctctatct ctcgctgcgc cacccggagc aggtccgcac cctggtcgca
     3841 cacgaggccc ccaccctgcg actgctgccg gcggccaccc gcgagcgggt cgtacggggg
     3901 gtggccgcgg ccgaacgcgc ctaccacaac gacggttggg gccccgccat gaagcagatg
     3961 gcggcactca gcggcatcga cctgaccacg ctggagatgg aaccgggcgt cgacttccaa
     4021 ccgatgacgg tcgaccgggc cgcgaacatc gagttcttcg tcaagtacga cggcccggcc
     4081 ttccgcaact gccagctcga cgccgacgac atcgcccggc tcaagcagac cggcatccgg
     4141 gtggtcccgg ccggcggctg catctcacag gacatctggg cgtacgtctg cgcggaggca
     4201 ctcgccgacg aactcggcac ggcggtggtg gagttccccg gtggccgcaa cggccacacc
     4261 acccaccccc gcgccttctc ggcacgcctg cacgaggtgc tcgccgccgt cgcctgactt
     4321 ccgcgcccga cacccaacct cgaacaggga cggccatcca tgggaagcac tgcccccgcg
     4381 taccaggacg ccatcgcggt catcggaatg tcctgccgat tccccggcgc cgcctccccg
     4441 gacgagttct gggacctgtt ggtacgcggg gaggaggcga tccgggaagc gccggccgac
     4501 cgctggtccg gcgacccggg cacggtccgt cgtggcggct tcctcgacga cgtggccggc
     4561 ttcgacgccc cgttcttcgg ggtgtcgccc cgcgaggccg ccgcgatgga cccgcagcag
     4621 cggttgatgc tggaactggc ctgggaggcg ctggagcacg ccggggccga cccgacgccg
     4681 ctgcgcggct cgtcgaccgg ggtgttcatc ggcacgatcc aggacgacta cgccaccctg
     4741 acccggcgcg gtggcggcga ccccggcccg cacacgctga ccgggctgca ccgcagcgtc
     4801 atcgccaacc gggtctccta ccggctgggc ctggccgggc cgagcatggt ggtcgacacc
     4861 ggccagtcgt cggccctggt ggcggtgcac cgggcgatcg agtcgctgca cgccggcgag
     4921 tgcacgatgg tgctcgccgg tggggtcaac ctgatcctca ccggggagag caccgacgcc
     4981 gtacgcggct tcggtggcct gtcacccacc gggcgtaccc acctgttcga cgcccacgcc
     5041 gacggctacg tccgcggtga gggcggtggc ctggtcgtgc tgaagcggct cgccgacgcg
     5101 gtcgccgacg gcgaccggat cttctgcgtg atccacggcg gtgccgtcaa caacgacggg
     5161 ggcggcgacg ggctgaccac gcccagccag gccggccagg agcaggtcgt ccagctcgcg
     5221 cacacccggg ccggcaccga cccggccgac accgggtacg tggaactgca cggcaccgga
     5281 accccggtgg gtgacccgat cgaggccgcc gcactcggtg cggtggtcgg cgcggcactg
     5341 gcacccggtg accggttgct ggtcggctcg gccaagacca atgtgggcca cctggagggc
     5401 gccgccgggg tggtggggct gatcaagacg gcgctcgccc tgcggcacgg gctgattccc
     5461 gcctccctgc atcatgagac gcccaacccg gcgatcgact tcgcggccac ccgactgacc
     5521 gtgcagacca ccctcgcggg ctggcccgtc acccggcctc tggccggcgt ctcgtcgttc
     5581 ggcatgggcg gcaccaactg ccacctggtc ctcggcccgt ggcccgcgcc gcccgccgcc
     5641 gttgacgccc cgacgtccgt cgacgggccc gggacgccgt cgggcacccc ggtggccgca
     5701 cccgccgtcg cccgggtcgt ctggccgctg tccggtcgta ccccggcggc gctgcgcgcc
     5761 caggcgcacc gcctgcacca ccatctcacc gaccggccca cggtgccgat cggcgatgtc
     5821 ggccgttcgc tggccggccg cagcggcttc gaccaccggg ccgtcgtggt cggccgcgac
     5881 cgtgcggaac tgctcgccgg gctggccgac ctcgccgacg gccgtcgccg ggcgggactg
     5941 gtcaccggtc gggccgacac cggcaccctg ggcttcctgt tcaccgggca gggcgcgcaa
     6001 cgccccggga tgggacgcga cctgtacgac gcctcgccgg tcttcgccga cgcgttcgac
     6061 gaggtgtgcg cggaactcga caggcacctg gaccggccgc tgcgcgaggt cgtctgggac
     6121 gatccgaccg cgctggacca gaccggctac acgcaacccg cgctcttcgc cctccagacg
     6181 gcgctccacc agctcaccgt ctccttcggg gtacgccccg actacctgct cggtcactcc
     6241 atcggtgaac tgtccgccgg gtacgccgcc agggtctggt ccctgcccga cgcctgtctg
     6301 ctcgtcgccg cccgtggccg actgatgcag gccgccccgg ccggcggcgc gatgctggcg
     6361 atcgccgcgc cggaggacgt ggtgaccgcc cacctcgccg acttcgccgc acggctggac
     6421 ctcgccgccg tgaacggccc gaacgcggtc gtcgtctccg gcgacgccga cgccgtcacc
     6481 gacctcgccg cccgcttcga ccagctcgcc gtcaagaccc gacggctccg ggtcagccac
     6541 gccttccact ccgcccacat ggacccggcg ctggacgagt tcacccgcgt cgcggccggc
     6601 atacccgccc ggactcccca ggtgccggtg gtgtcgaacg tgtccgggaa gctggtgcag
     6661 gccgaataca ccgacccgac gtactgggcg gcgcagatcc gtcgacccgt ccgtttcctc
     6721 gacggcctgc gtacgctgcg gtcggccggg gtcaccgcgt tcctggaact cggcccggac
     6781 ccggtgctga ccgggctggc cgaggcggag caggacgccg gccccggcag ctccgacgac
     6841 acggcgtacg ccgtcctgct gcggcgggac cgcgacgagc cgacgacggc gttgaccgcg
     6901 ctggcccggc tgcactccca cggtgtgccg gtgaactggt ccccactgtg gccggacgcg
     6961 gaccgggccg acctgccgac ctacgcgttc cagcggcgac cgtactggat cgacaccgcc
     7021 gcctcctccg ccgccgctgc ctcctccgcc ggctccgccg tgccggtcga cgcgccttcg
     7081 tctaccgcgc cggaagagcc cgcgaccggg gcggcggagg tcgacccggc cagctgggct
     7141 gggcggctgt tggcgctgcc cgagcacgag cgccggcccg ccgccgaact gctcgtacgt
     7201 cgcctggtgg ccgagacgct cgactatgcc gacctggccg acgtcgacgg cagtctcacc
     7261 ttcaaggaac tcggcatcac gtcgctcacc gccgtggaac tacgcaacct cgtgaccgcc
     7321 gaaaccggcc agtcgttctc cgcctccatg gtcttcgact ttccgactcc ggtgttggtg
     7381 gcggagcggt tggtgggttt ggtggtgggt ttgggttcgg gtgtgggtgt tgcgggtgtg
     7441 ggtgtgggtg aggcggtggc gattgtgggg atggggtg
//
