LOCUS       NZ_JAGFWR010000047      9108 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Micromonospora antibiotica strain MMS20-R2-23 contig47, whole genome
            shotgun sequence.
ACCESSION   NZ_JAGFWR010000047
VERSION     NZ_JAGFWR010000047.1
KEYWORDS    WGS; RefSeq.
SOURCE      Micromonospora antibiotica
  ORGANISM  Micromonospora antibiotica
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Lee,D.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2021) Microbiology & Molecular Biology, Chungnam
            National University, 99, Daehak-ro, Yuseong-gu, Daejeon 34134, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.63x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017599305.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 02:05:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,561
            CDSs (total)                      :: 6,498
            Genes (coding)                    :: 6,378
            CDSs (with protein)               :: 6,378
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 20 of 120
            Pseudo Genes (incomplete)         :: 107 of 120
            Pseudo Genes (internal stop)      :: 2 of 120
            Pseudo Genes (multiple problems)  :: 9 of 120
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:14:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9108
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAGFWR010000047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9108
                     /collection_date="2018-03-14"
                     /db_xref="taxon:2807623"
                     /geo_loc_name="South Korea: Sejong-si"
                     /isolation_source="riverside soil"
                     /mol_type="genomic DNA"
                     /organism="Micromonospora antibiotica"
                     /strain="MMS20-R2-23"
                     /submitter_seqid="contig47"
                     /type_material="type strain of Micromonospora antibiotica"
     region          1..9108
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9108
                     /SMILES="NC(CCC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9108
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[3789:7800](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3790..7800
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     misc_feature    23..38
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.19"
                     /tool="antismash"
     misc_feature    complement(33..46)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.65"
                     /tool="antismash"
     misc_feature    complement(110..119)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.67"
                     /tool="antismash"
     gene            complement(164..1060)
                     /locus_tag="JQN83_RS32365"
                     /old_locus_tag="JQN83_32360"
     CDS             complement(164..1060)
                     /EC_number="3.8.1.5"
                     /GO_function="GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (39-173). E-value:
                     6.3e-27. Score: 86.7. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32365_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /locus_tag="JQN83_RS32365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32360"
                     /product="haloalkane dehalogenase"
                     /protein_id="WP_219463149.1"
                     /sec_met_domain="PF00561 (E-value: 7.8e-25, bitscore: 76.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.7e-14, bitscore:
                     42.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQSSMPAAVPVTVADPHPRRRIDLCGTHLSYVDVGDGEPIVFLH
                     GNPTSSYLWRNVIPAVRDLGRCLAPDLVGMGDSGPAPDGGYRFTDQARYLDAWFDAVVG
                     DRPVTLVLHDWGSALGFHWAARHPERVRAVAYLEAIVQPRRWSDFEAGRDQLFRAMRSP
                     DGERLVLQENFFVEVVLPRSVVRPLSEAELDHYRRPFGDPDSRRPTLMFPRELPIEGEP
                     ADVTAIVEEYGRWLAASPVPKLLVVAEPGALLTGRALAFARTFPNQREVTVTGSHYVQE
                     DSPDEIGAALRDFVTAL"
     aSDomain        complement(542..943)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32365_Abhydrolase_1.1"
                     /evalue="6.30E-27"
                     /label="JQN83_RS32365_Abhydrolase_1.1"
                     /locus_tag="JQN83_RS32365"
                     /protein_end="173"
                     /protein_start="39"
                     /score="86.7"
                     /tool="antismash"
                     /translation="PIVFLHGNPTSSYLWRNVIPAVRDLGRCLAPDLVGMGDSGPAPDG
                     GYRFTDQARYLDAWFDAVVGDRPVTLVLHDWGSALGFHWAARHPERVRAVAYLEAIVQP
                     RRWSDFEAGRDQLFRAMRSPDGERLVLQEN"
     gene            1457..2140
                     /locus_tag="JQN83_RS32370"
                     /old_locus_tag="JQN83_32365"
     CDS             1457..2140
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012900.5"
                     /locus_tag="JQN83_RS32370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32365"
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="WP_208570956.1"
                     /transl_table=11
                     /translation="MQGLIQVAGVIDQEEADLICAEGADWLGFALRLPAKNEDLSEADA
                     AAIVAAIQPRHRGVLITYLTEAEEIRQFCAELGVTAVQLHGDVDPAQLEKLRRIDPDLY
                     VLKSLVVKSDNLDELVKIVDESAEWVDMYITDSFNPATGAKGATGLVHDWSVSAELVRV
                     APRPLMLAGGLNPDNVAAAIAAVRPAAVDAHTGLEGPDRRKDRAKVRQFVTAARQAFAD
                     LAGQD"
     gene            2146..2853
                     /gene="upp"
                     /locus_tag="JQN83_RS32375"
                     /old_locus_tag="JQN83_32370"
     CDS             2146..2853
                     /EC_number="2.4.2.9"
                     /GO_function="GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="upp"
                     /inference="COORDINATES: protein motif:HMM:NF026032.5"
                     /locus_tag="JQN83_RS32375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32370"
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="WP_208570957.1"
                     /transl_table=11
                     /translation="MTDTAIHPTVTDPAVAGPAVASRLHLLPQTNRLRSLHTVIRDRDA
                     SRADFVQTSAQIFRQLLDTALDLLPVVPQQVRTPVGQTYHGLRPAAPVCGVSIARAGES
                     MEGELRAMLPQTRIGKILIQRDKTTKLPHFHYAALPPDIAMSQVLLLDPMLATGGTALA
                     ALALLREYGVFEEDVVFVTLLSSPEGISAVHAQHPRVRIVTSAIEERLNADAYMVPGIG
                     DFGDRYFGTDQPR"
     gene            2850..3764
                     /locus_tag="JQN83_RS32380"
                     /old_locus_tag="JQN83_32375"
     CDS             2850..3764
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510107.1"
                     /locus_tag="JQN83_RS32380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32375"
                     /product="EamA family transporter"
                     /protein_id="WP_208570958.1"
                     /transl_table=11
                     /translation="MRRGGIGDVGITALAPAIWGSTYLVTTELLPPGRPYLAALARALP
                     AGLALMALGRVVPRGAWIWRALVLGTLNIGLFFVLLFIAAYRLPGGVAALIMSFQPMLV
                     LILAALLLRDRIRPLHVMACLLGATGVALLVLRPTAALDPLGVIAALAGAASMASGIVL
                     TKRWARPPGVGVLQFTGWQLTAGGLVLLPTTLLMEGLPDRLTGPNLAGLAYLGIVGSLF
                     GYAVWFRGLERLPALAASFLSLVSPIVASLLGFAFLDQRLSVTQLVGALVIVAAVALAQ
                     RRSSPPAPPPDPVPVASRALTSR"
     gene            3790..7800
                     /locus_tag="JQN83_RS32385"
                     /old_locus_tag="JQN83_32380"
     CDS             3790..7800
                     /NRPS_PKS="Domain: AMP-binding (36-433). E-value: 2.2e-106.
                     Score: 347.8. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32385_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (944-1013). E-value: 1.6e-19. Score:
                     61.9. Matches aSDomain: nrpspksdomains_JQN83_RS32385_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1031-1146). E-value:
                     1.2e-22. Score: 73.4. Matches aSDomain:
                     nrpspksdomains_JQN83_RS32385_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="JQN83_RS32385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32380"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_208570959.1"
                     /sec_met_domain="AMP-binding (E-value: 7.9e-113, bitscore:
                     365.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-16, bitscore:
                     50.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 7.8e-23, bitscore:
                     70.7, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 6.6e-08, bitscore: 21.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTTPATTEAHQSTTDTLEPATGTHDPADVTLPDLLDEQVRADPD
                     AIAVRHGPEGITRRELARRARTLAAQLRERGTGPDDRIGLFAEPSAALVVGAWGILYAS
                     AAYLPLAPDYPDERLRYMATDAGLRVVVAQDDLVERLSDLVPPGTAIVPLSVWHDTAPP
                     DVPRRGPRPGHLAYVIYTSGSTGRPKGVMIEHRSIVSQLRWLRDTYRLDEAVILQKTPM
                     SFDAAQWEILAPACGGTVVAGTPGLHRDPEAIIETIRRHGVTALQCVPTLLRALVDTER
                     LGTCTSLRQIFTGGEALSTTLAADVLAAHPATLVNLYGPTECTINSSAFTVDPATLDPT
                     QQSVSIGRPVTGLRYVIVDTDDAPVTGEAVGELLIGGVQLARGYLHRPALTAQRFVPDP
                     VTGERLYRTGDLAAWQADGTVRFVGRVDNQVKLRGFRVELDEVRLAVEAHQWVRHAGVL
                     VRDDPRTGHPHLLAVVELNPREAALMDQGRQGAHHRSKESRLQVRAQLSHGGVRTPQEL
                     AGRPVLALPGAEPTEEQVRRVFARKTYRFYEGPPVTRADLLALLRPAEPGPVPPRPLAQ
                     LDLATFGEIIRYLGQFVSDERLLPKYGYASPGALNATQVYLEIHQLWGLPSGYYYHHPL
                     THELVRIGPAASRPEPTVRLHLVGRRRAIEPVYQNNIREVLEIETGHLVGLLEQVLPGY
                     GLTVTAADHTPQTIRHLDCADEDLYLGSFALTPYAPPPPDEIDVYVQVHPDRGGDLAPG
                     QYLHRDGDLVRIDDALVLRQHVIAINQQVYDRAAIGISMISTTSTRWRRYVDLGRALQR
                     LSMNDLGFGFMSSGYSSDTGHDLPSAHRIRGILRRHGLPDGPSYFAVGGRVSAAQVAGR
                     GMKEDVVHMKGPAELIRDDLAGRLPDYMLPNKVVIVDRMPLTANGKTDLTALAARVADD
                     PGPQRPFVAPRTDLERRIARLWAEAVRQDDVSVHDDFFACGGNSLIAVALINRLNRVFG
                     ATLPMQVIFDSPTVAQLARRVSQRDQVSRSRLVPLAPAREGRSVYCWPGLGGYPMNLRL
                     LAGTVGRPFHGVQAHGVNPGEEPYRTIARMAAEDVRAIRERQPRGPYTLWGYSFGTRVA
                     VEAAYQLEQAGEQVDQLVLIAPGSPRVGVPQDPATVGTASFANPTYLAVLCSVFAGTVS
                     GPVIEECLATVVDEESFTGFVQRHFPALDLPLVRRITGVVARTYGLRHTEDELARRPVR
                     APVTVLRTRGDQESFLDTPVARRNLSPVVVDLAADHYGVLREPGVHQVVAALPARSRRR
                     PVRPHVSISHLPIALTDEQHNRLVTAVTRAMTRAFGCDEDAVSVALEAVLEE"
     aSDomain        3898..5088
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32385_AMP-binding.1"
                     /evalue="2.20E-106"
                     /label="JQN83_RS32385_AMP-binding.1"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="433"
                     /protein_start="36"
                     /score="347.8"
                     /specificity="substrate consensus: Gln"
                     /tool="antismash"
                     /translation="DEQVRADPDAIAVRHGPEGITRRELARRARTLAAQLRERGTGPDD
                     RIGLFAEPSAALVVGAWGILYASAAYLPLAPDYPDERLRYMATDAGLRVVVAQDDLVER
                     LSDLVPPGTAIVPLSVWHDTAPPDVPRRGPRPGHLAYVIYTSGSTGRPKGVMIEHRSIV
                     SQLRWLRDTYRLDEAVILQKTPMSFDAAQWEILAPACGGTVVAGTPGLHRDPEAIIETI
                     RRHGVTALQCVPTLLRALVDTERLGTCTSLRQIFTGGEALSTTLAADVLAAHPATLVNL
                     YGPTECTINSSAFTVDPATLDPTQQSVSIGRPVTGLRYVIVDTDDAPVTGEAVGELLIG
                     GVQLARGYLHRPALTAQRFVPDPVTGERLYRTGDLAAWQADGTVRFVGRVDNQVKLR"
     aSModule        3898..7227
                     /complete
                     /domains="nrpspksdomains_JQN83_RS32385_AMP-binding.1"
                     /domains="nrpspksdomains_JQN83_RS32385_PCP.1"
                     /domains="nrpspksdomains_JQN83_RS32385_Thioesterase.1"
                     /final_module
                     /locus_tags="JQN83_RS32385"
                     /monomer_pairings="Gln -> Gln"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4315..4374
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32385_0001"
                     /evalue="1.10E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="195"
                     /protein_start="175"
                     /score="36.3"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVMIEH"
     CDS_motif       4894..4974
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32385_0002"
                     /evalue="6.00E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="395"
                     /protein_start="368"
                     /score="47.3"
                     /tool="antismash"
                     /translation="AVGELLIGGVQLARGYLHRPALTAQRF"
     CDS_motif       5053..5115
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32385_0003"
                     /evalue="7.10E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="442"
                     /protein_start="421"
                     /score="31.0"
                     /tool="antismash"
                     /translation="FVGRVDNQVKLRGFRVELDEV"
     aSDomain        6622..6828
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32385_PCP.1"
                     /evalue="1.60E-19"
                     /label="JQN83_RS32385_PCP.1"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="1013"
                     /protein_start="944"
                     /score="61.9"
                     /tool="antismash"
                     /translation="ERRIARLWAEAVRQDDVSVHDDFFACGGNSLIAVALINRLNRVFG
                     ATLPMQVIFDSPTVAQLARRVSQR"
     aSDomain        6883..7227
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQN83_RS32385_Thioesterase.1"
                     /evalue="1.20E-22"
                     /label="JQN83_RS32385_Thioesterase.1"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="1146"
                     /protein_start="1031"
                     /score="73.4"
                     /tool="antismash"
                     /translation="SVYCWPGLGGYPMNLRLLAGTVGRPFHGVQAHGVNPGEEPYRTIA
                     RMAAEDVRAIRERQPRGPYTLWGYSFGTRVAVEAAYQLEQAGEQVDQLVLIAPGSPRVG
                     VPQDPATVGTA"
     CDS_motif       7057..7131
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JQN83_RS32385_0004"
                     /evalue="6.90E-10"
                     /label="NRPS-te1"
                     /locus_tag="JQN83_RS32385"
                     /protein_end="1114"
                     /protein_start="1089"
                     /score="31.3"
                     /tool="antismash"
                     /translation="QPRGPYTLWGYSFGTRVAVEAAYQL"
     gene            7942..8970
                     /locus_tag="JQN83_RS32390"
                     /old_locus_tag="JQN83_32385"
     CDS             7942..8970
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016142.5"
                     /locus_tag="JQN83_RS32390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32385"
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="WP_208570960.1"
                     /transl_table=11
                     /translation="MTRNVHSTVEVRLSEEVADALAEGRAVVTLESNVITHGLRYPDNA
                     ATAHRVEDAVREGGAVPATVAVDDGVIRVGMTAADIERFASTPGIPKVSARDLPVALAR
                     GGMGALTVASSLIAADLAGIAFFASAGIGGVHRGAETSMDISSDLVQFTRSRVAVVCAG
                     AKKILDLGLTMEYLETHSVPVVSYRSDDFPAFYCVSSGIPSPCRLDDDTVVARAVENHW
                     ALGNHSSFLITTPVDAEEAVDDAEVDRAIVEAMSAARRDGVRGNAITKYLMRAVDAATQ
                     GRSSRANMAVLISTARTGGHLAAAHARYLAEVGNPATAGAAPPARVPAGAGTAHALPGA
                     RT"
     gene            8986..>9108
                     /locus_tag="JQN83_RS32395"
                     /old_locus_tag="JQN83_32390"
     CDS             8986..>9108
                     /EC_number="4.2.3.5"
                     /GO_function="GO:0004107 - chorismate synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905738.1"
                     /locus_tag="JQN83_RS32395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQN83_32390"
                     /product="chorismate synthase"
                     /protein_id="WP_208570962.1"
                     /transl_table=11
                     /translation="MLRWLTAGESHGPALVALLEGVPAGVAVTSGDIAGELARRR"
ORIGIN
        1 ctcctcatcg aggaccctgc ccattgatga tctttgtgtg agaactcaga tcatggacag
       61 ggtcctcccc attccgctag acctccacca ccacgaatcc ccccgacggt caaaacacga
      121 gtccgtgggg cgggcggttc caccgcccgc cccacggctg tcctcagagg gcggtgacga
      181 agtcccgcag ggcggccccg atctcgtccg gtgagtcctc ctgcacgtag tggctgccgg
      241 tgacggtgac ctcgcgctgg ttggggaagg tccgggcgaa cgccagggcc cggccggtga
      301 gcaacgcccc cggctcggcg accaccagca gcttcggcac cggggaggcg gccagccagc
      361 ggccgtactc ctcgacgatg gcggtcacgt cggccggttc gccctcgatg ggcagctccc
      421 gggggaacat cagggtcggt cggcgcgagt cggggtcgcc gaagggccga cggtagtggt
      481 ccagttccgc ctccgacagg gggcgcacca cgctacgcgg cagcaccacc tcgacgaaga
      541 agttctcctg gagcaccagg cgttccccgt cgggcgagcg catggcgcgg aagagctggt
      601 cccggccggc ctcgaagtcg ctccaccggc ggggctggac gatcgcctcc aggtaggcga
      661 cggcccggac ccgttcggga tggcgggccg cccagtggaa gcccagcgcc gaaccccagt
      721 cgtgcagcac cagggtgacc ggtcggtcgc ccaccacggc gtcgaaccag gcgtcgagat
      781 agcgcgcctg gtcggtgaag cggtagccgc cgtcgggcgc cggcccggag tcgcccatcc
      841 cgaccaggtc gggggcgagg cagcggccca ggtcgcggac cgccgggatg acgttgcgcc
      901 acaggtacga ggacgtgggg ttgccgtgca ggaacacgat cggttccccg tcgcccacgt
      961 cgacgtacga gaggtgggtc ccgcacaggt cgatccgacg gcgggggtgt gggtcggcca
     1021 cggtcacggg caccgcggcg ggcatggaac tctgtgtcac ttgtagacct cctcggcccc
     1081 agtgatagca agccggcccg gcaattcccg ggaatccccg tgacggcgtg tccgtcgacg
     1141 ggcgacagtt cgcgctcaat gcggcggtgc cattcacgaa ctgttcgcga gaaatcaacg
     1201 aatgacttgg tgaatccgat tcggctccgc cgcgatcagg cggtacgggg tgccgtggtg
     1261 ccatggccgg gccactcgcc ggcaaagtgg caccgacgca ttcccgcaca tgggcctgtc
     1321 caccggcacc gcagtttcgt acgtttctcc tgtcgatacg agggcccgga cgagtccttc
     1381 tcggatctcc ggacgccacc gatgtgtcaa tgcgccacgc gggatttccg ccggctttcc
     1441 agaaagggag aaccagatgc agggactgat ccaggtcgcc ggggtcatcg accaggagga
     1501 ggccgacctg atctgtgcgg agggcgccga ctggctgggg ttcgccctgc ggttgccggc
     1561 gaagaacgag gacctgtccg aggctgacgc ggccgccatc gtcgcggcga tccagccccg
     1621 gcaccggggc gtcctgatca cctacctcac cgaggccgag gagatcaggc agttctgcgc
     1681 cgaactcggc gtcaccgccg tgcagctgca cggcgacgtg gaccccgccc agttggagaa
     1741 gctgcgccgg atcgaccccg acctctacgt gctcaagagc ctcgtggtca agtccgacaa
     1801 cctcgacgag ctggtcaaga tcgtcgatga gagcgccgag tgggtcgaca tgtacatcac
     1861 cgacagcttc aaccccgcca ccggggccaa gggcgcgacc ggtctggtcc acgactggtc
     1921 ggtcagcgcc gagctggtcc gcgtcgcgcc ccgaccgttg atgctcgccg gtggcctgaa
     1981 ccccgacaac gtggcggcgg ccatcgcggc ggtacgcccc gccgccgtcg acgcgcacac
     2041 cgggctggag ggccccgacc gccgcaagga ccgcgccaag gtgcgccagt tcgtcaccgc
     2101 cgcccgccag gcgttcgccg acctcgccgg acaggactga gagccatgac cgacaccgcc
     2161 atccacccca ccgtgaccga ccccgccgtg gccggaccgg ccgtggccag ccggctgcac
     2221 ctgttgccgc agaccaaccg gctgcgctcg ctgcacaccg tcatccggga ccgcgacgcc
     2281 tcccgggccg acttcgtgca gacctccgcc cagatcttcc ggcagctcct cgacaccgcc
     2341 ctcgacctgc tcccggtcgt cccccagcag gtgcgcaccc cggtcgggca gacctaccac
     2401 gggctgcgcc cggccgcacc ggtctgcggg gtgtccatcg cccgcgccgg tgagagcatg
     2461 gagggcgaac tgcgggccat gctgccgcag acccggatcg gcaagatcct catccagcgg
     2521 gacaagacga cgaaactgcc gcacttccac tacgcggccc tgcccccgga catcgccatg
     2581 tcccaggtgc tgctgctgga cccgatgctc gccaccggcg gcaccgcgct ggccgccctc
     2641 gcgctgctac gtgagtacgg ggtcttcgag gaggacgtcg tcttcgtcac cctgctctcc
     2701 tcgccggagg gcatcagcgc cgtgcacgcc cagcaccccc gggtccggat cgtcacctcc
     2761 gccatcgagg agcgcctcaa cgccgacgcc tacatggtgc ccggcatcgg cgacttcggt
     2821 gaccgctact tcggcacgga ccagccccga tgaggcgcgg cggaatcggc gacgtcggga
     2881 tcaccgcgct ggcccccgcg atctggggca gcacctacct cgtcaccacc gagttgctgc
     2941 ccccgggccg gccctacctg gccgccctgg cccgtgccct gcccgccggg ctcgccctga
     3001 tggcgctcgg gcgggtggtg ccgcgcggcg cgtggatctg gcgggcgctg gtgctcggca
     3061 ccctcaacat cggtctgttc ttcgtgctgc tcttcatcgc ggcctaccgg ctgcccggtg
     3121 gcgtcgcggc cctgatcatg tcgttccagc cgatgctggt gctgatcctc gcggcgctgc
     3181 tgctgcgcga ccgcatccgg ccgctgcacg tcatggcgtg cctgctcggg gcgaccgggg
     3241 tcgcgctgct cgtgctgcgc ccgaccgccg cgctcgaccc gctcggcgtg atcgcggccc
     3301 tggccggcgc ggccagcatg gccagcggga tcgtgctgac caaacgctgg gcgcgaccgc
     3361 cgggggtggg ggtgttgcag ttcaccggtt ggcagctgac cgccggcggg ctggtcctgc
     3421 tccccaccac cctgctgatg gaagggctgc cggaccgcct caccggtccc aacctggccg
     3481 gcctcgccta cctggggatc gtcggatcgc tgttcggcta cgcggtctgg ttccgcgggc
     3541 tggaacggct gcccgccctg gccgcctcct tcctcagcct ggtcagcccg atcgtcgcca
     3601 gcctgctggg cttcgccttc ctcgaccaac gactcagcgt cacccagctc gtcggagccc
     3661 tggtcatcgt cgccgcagtc gccctcgccc agcgcaggtc gtccccgccg gctccgccac
     3721 ccgacccggt gccggtggcg tcacgggcgc tgaccagccg gtgaggcgtc acccaccaca
     3781 gggggaagca tgcttaccac gcccgccacc accgaagccc accagtcgac caccgacacc
     3841 cttgaaccgg ccaccggcac gcacgacccg gccgacgtga ccctgccgga cctgctcgac
     3901 gagcaggtcc gggcggaccc cgacgcgatc gccgtccggc acggaccgga ggggatcacc
     3961 cgacgggaac tggcccgccg ggcccgcacc ctcgcggccc agctccgcga gcggggcacc
     4021 ggccccgacg accggatcgg actcttcgcc gagccgtccg ccgcactcgt cgtcggggcc
     4081 tggggaatcc tgtacgcgtc ggccgcctac ctgcccctgg cccccgacta tcccgacgaa
     4141 cgcctgcgct acatggccac cgacgccggg ttgcgcgtcg tcgtcgccca ggacgacctc
     4201 gtcgaacggc tgtcggacct ggtcccgccc ggcaccgcca tcgtgccgct gagcgtgtgg
     4261 cacgacacgg ccccacccga cgtgccccgc cgggggccac ggcccggcca tctcgcctac
     4321 gtcatctaca cctccggcag caccggtcga cccaagggcg tgatgatcga gcaccgcagc
     4381 atcgtcagcc aactgcggtg gctgcgggac acctaccgcc tcgacgaggc cgtcatcctc
     4441 cagaagaccc cgatgagctt cgacgcggca cagtgggaga tcctcgcccc ggcctgcggc
     4501 ggcaccgtcg tcgccggcac cccgggcctg caccgcgacc ccgaggccat catcgagacc
     4561 atccgacggc acggcgtcac cgccctgcag tgcgtaccga ccctgctgcg ggcgctggtc
     4621 gacaccgaac gcctcggcac ctgcaccagc ctgcggcaga tcttcaccgg tggtgaggcg
     4681 ctgtccacca cactcgccgc cgacgtgctc gcggcgcacc ccgccaccct ggtcaacctc
     4741 tacggcccca ccgagtgcac gatcaactcg tcggcgttca ccgtcgaccc ggcgaccctc
     4801 gaccccacgc agcagtccgt gtcgatcggg cggcccgtca ccggcctgcg ctacgtcatc
     4861 gtcgacaccg acgacgcccc ggtcaccggg gaggccgtcg gggaactgct catcggcggc
     4921 gtccagctcg cccggggcta cctgcaccgg ccggcgttga ccgcgcaacg gttcgtgccc
     4981 gacccggtca ccggcgaacg gctctaccgc accggtgacc tggccgcctg gcaggccgac
     5041 ggcaccgtac ggttcgtcgg tcgggtcgac aaccaggtga agctgcgcgg gttccgggtc
     5101 gaactcgacg aggtgcgcct cgccgtggag gcccaccagt gggtccggca cgcgggggta
     5161 ctggtccgcg acgacccgcg taccggccac ccgcacctgc tcgccgtcgt cgaactcaac
     5221 ccgcgtgagg cggcgctgat ggaccagggg cggcaggggg cccaccaccg gtcgaaggag
     5281 agccggctcc aggtccgggc ccagttgtcg cacggcgggg tgcgtacccc gcaggagctg
     5341 gccgggcggc cggtgctcgc actgcccggg gccgaaccca ccgaggagca ggtacggcgg
     5401 gtgttcgccc gcaagacgta ccggttctac gagggcccgc cggtcacccg cgccgacctg
     5461 ctggcactgc tgcgcccggc cgagcccggc ccggtgccgc cgcgcccgct cgcacagctc
     5521 gacctggcca ccttcgggga gatcatccgc tacctcgggc agttcgtcag cgacgagcgg
     5581 ctgctgccca agtacggcta cgcctcaccg ggggcgctca acgccaccca ggtctacctg
     5641 gagatccacc agctgtgggg gctaccgtcc ggctactact accaccatcc gctcacccac
     5701 gaactggtcc ggatcggccc ggccgcgtcc cggcccgaac cgaccgtccg gctgcacctc
     5761 gtcggccggc gacgggccat cgaaccggtc taccagaaca acatccgcga ggtgttggag
     5821 atcgagaccg gccacctggt cggcctgctg gaacaggtcc tgcccgggta cgggctgacc
     5881 gtcacggccg ccgaccacac cccgcagacc atccggcacc tcgactgcgc cgacgaggac
     5941 ctctacctcg gcagcttcgc gctgacgccg tacgccccgc caccgcccga cgagatcgac
     6001 gtgtacgtcc aggtccaccc ggaccggggc ggggacctgg ccccggggca gtacctgcac
     6061 cgtgacggcg acctggtccg catcgacgac gccctggtgc tgcgccagca cgtcatcgcg
     6121 atcaaccagc aggtgtacga ccgggccgcg atcggcatca gcatgatcag caccacgtcg
     6181 acccggtggc ggcgctacgt cgacctgggc cgcgccctgc aacggctgtc catgaacgac
     6241 ctcggcttcg gcttcatgtc ctccggctac agctccgaca ccggtcacga cctgccctcg
     6301 gcgcaccgca tccggggcat cctgcgccgg cacgggctac ccgacggtcc gtcgtacttc
     6361 gccgtgggtg ggcgggtcag tgccgcccag gtggccggcc ggggcatgaa ggaggacgtc
     6421 gtacacatga agggtccggc ggagctgatc cgcgacgacc tggccggtcg gctgccggac
     6481 tacatgctgc ccaacaaggt ggtgatcgtc gaccggatgc cgctgaccgc caacggcaag
     6541 accgacctca cggcactcgc cgcccgggtc gccgacgacc ccggcccgca acggccgttc
     6601 gtggcgcccc gcaccgacct ggaacgccgc atcgcccgcc tgtgggccga ggcggtccgc
     6661 caggacgacg tctccgtcca cgacgacttc ttcgcctgcg gtggcaactc gctgatcgcc
     6721 gtcgccctga tcaaccgact caaccgggtg ttcggcgcga cgctgcccat gcaggtcatc
     6781 ttcgacagcc cgacggtggc ccagctggcc cggcgcgtct cccaacgcga ccaggtcagc
     6841 aggtcccggc tggtgccgtt ggcgccggcc cgggagggtc gctccgtcta ctgctggccg
     6901 ggcctcggcg gctacccgat gaacctgcgg ctgctcgccg gcacggtcgg ccggcccttc
     6961 cacggcgtgc aggcccacgg cgtgaacccg ggcgaggagc cctaccggac gatcgcccgg
     7021 atggcggccg aggacgtgcg ggcgatccgg gaacggcaac cccgtggccc ctacacgctg
     7081 tggggttact cgttcggcac ccgggtcgcg gtggaggcgg cgtaccagtt ggagcaggcc
     7141 ggcgagcagg tcgaccaact cgtcctgatc gcccccggat caccccgggt aggggtgccg
     7201 caggacccgg ccacggtcgg cacggcgagc ttcgccaacc cgacgtacct ggccgtgctg
     7261 tgttccgtgt tcgcggggac ggtcagcggg ccggtgatcg aggagtgcct ggccaccgtc
     7321 gtggacgagg agagcttcac cgggttcgtc caacggcact tccccgccct ggacctgccg
     7381 ctggtccgcc ggatcaccgg ggtcgtcgcc cgcacgtacg ggctgcgcca caccgaggac
     7441 gaactcgcca ggcggccggt gcgggccccg gtgacggtgc tacgcacccg gggtgaccag
     7501 gagtccttcc tcgacacccc cgtcgcccgc cgcaacctct cccccgtcgt cgtcgacctg
     7561 gccgccgacc actacggggt gctgcgcgag ccgggggtcc accaggtggt ggcggcgctg
     7621 cccgcccggt cccggcgtcg cccggtccga ccgcacgtga gcatcagcca cctgccgatc
     7681 gccctgaccg acgagcagca caaccggctg gtgaccgccg tcacccgcgc catgaccaga
     7741 gccttcggct gcgacgagga cgcggtctcc gtcgcactgg aagcggtcct ggaggagtga
     7801 gccggtcccc ggcccacacc gcccccgacc caccgacggg ccggccccgc cgacgacccg
     7861 caaccgacct gcccgaaacc ggtcccgagc tgcccgacac cgaacccgac cggcccggac
     7921 cggaccgacc gaggagcccg catgacacgc aacgtccact ccaccgtcga ggtgcggctg
     7981 agtgaggagg tcgccgacgc ccttgccgag gggcgggcgg tggtgacgct ggaatccaac
     8041 gtgatcaccc acggcctgcg ttacccggac aacgccgcca ccgcgcaccg ggtcgaagat
     8101 gccgtccggg agggcggcgc ggtgccggcg acggtcgcgg tcgacgacgg ggtgatccgg
     8161 gtcggcatga ccgcagcgga catcgaacgg ttcgcctcca caccgggcat ccccaaggtg
     8221 agcgcccggg acctgccggt ggcgctcgcc cggggcggca tgggcgcgct gaccgtggcg
     8281 agttcactga tcgcggcgga cctcgccggc atcgcgttct tcgcgtccgc cggcatcggc
     8341 ggggtccacc gcggcgcgga gaccagcatg gacatctcct cggacctcgt ccagttcacc
     8401 cgttcccggg tcgccgtggt gtgcgccggg gccaagaaga tcctcgacct cggactgacc
     8461 atggaatatc tggagaccca ctcggtgccg gtggtctcct accgctccga cgactttccg
     8521 gccttctact gcgtctccag cggcatcccc agcccctgcc gcctcgacga cgacacggtc
     8581 gtcgcccgcg cggtggagaa ccactgggcg ctgggcaacc acagctcctt cctgatcacc
     8641 accccggtcg acgccgagga ggcggtggac gacgccgagg tcgaccgggc catcgtcgag
     8701 gcgatgtcgg ccgcccgacg cgacggggta cgcggcaacg ccatcacgaa gtacctcatg
     8761 cgtgctgtcg acgcggccac ccagggccgc tcgtcccggg ccaacatggc ggtgctgatc
     8821 agcaccgccc ggaccggcgg ccacctcgcc gccgcccacg cccgctacct ggccgaggtg
     8881 gggaacccgg ctaccgccgg tgccgccccg ccggcccggg tgccggccgg cgccgggacg
     8941 gcgcacgccc tgcccggtgc gcggacctga cggggggtgg tgacggtgtt gcgttggctg
     9001 actgcaggtg aatcgcatgg tccggctctg gtggcgttgt tggagggggt tcccgctggg
     9061 gtggcggtga cttctggtga catcgcgggt gagttggcgc ggcgtcgg
//
