LOCUS       NZ_JAFJXZ010000021      6252 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Paenibacillus polymyxa strain Hob6 Scaffold_21, whole genome shotgun
            sequence.
ACCESSION   NZ_JAFJXZ010000021
VERSION     NZ_JAFJXZ010000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Draft genome sequence of Paenibacillus polymyxa strain Hob6,
            isolated from hemp (Cannabis sative) seeds' oil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2021) Plant Science Departement, McGill
            University, 21111 Lakeshore, te-Anne-de-Bellevue, Quebec H9X 3V9,
            Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2020
            Assembly Method                   :: SPAdes v. 3.12.0; Medusa v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly :: GCA_000507205.2; GCA_014706575.1;
            GCA_000164985.2; GCA_000597985.1; GCA_002893885.1
            Genome Coverage                   :: 266.29x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017589695.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:57:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,119
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 156
            rRNAs                             :: 21, 9, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 19, 2 (5S, 16S)
            partial rRNAs                     :: 2, 7, 15 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 44 of 95
            Pseudo Genes (incomplete)         :: 63 of 95
            Pseudo Genes (internal stop)      :: 13 of 95
            Pseudo Genes (multiple problems)  :: 21 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6252
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAFJXZ010000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6252
                     /collected_by="Mohannad Mahmoud, Suha Jabaji"
                     /collection_date="2019-11-18"
                     /db_xref="taxon:1406"
                     /host="Cannabis sativa"
                     /identified_by="Mohannad Mahmoud"
                     /isolation_source="commercial industrial hemp seed oil"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus polymyxa"
                     /strain="Hob6"
                     /submitter_seqid="Scaffold_21"
     region          1..6252
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6252
                     /SMILES="NC(CCCN)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6252
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[835:>6252](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      836..>6252
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            50..>255
                     /locus_tag="JYU28_RS25040"
                     /pseudo=""
     CDS             50..>255
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="JYU28_RS25040"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="peptide synthetase"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 9.6e-10, bitscore:
                     25.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAIEKNKVTTMHFVPSMLSVFLEYVKEKGIIEEIASLKQVFASG
                     EALNLKQVEKFNKLLYKEKGTSL"
     assembly_gap    256..355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     gene            <356..>724
                     /locus_tag="JYU28_RS25045"
                     /old_locus_tag="JYU28_25085"
     CDS             360..>724
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="JYU28_RS25045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_25085"
                     /product="AMP-binding protein"
                     /protein_id="WP_208332769.1"
                     /sec_met_domain="AMP-binding (E-value: 3.2e-35, bitscore:
                     109.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALNLKQVEKFNKLLYKEKGTSLHNLYGPTEATVDVSYFGCSTEE
                     ELELVPIGKPIDNIKLYVVSKEKGLQPIGVAGELCIAGDGLARGYLNRPELTEEKFAAN
                     PFAPGERMYRTGDLARWL"
     assembly_gap    725..824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     gene            836..>6252
                     /locus_tag="JYU28_RS25050"
                     /old_locus_tag="JYU28_25090"
     CDS             836..>6252
                     /NRPS_PKS="Domain: PCP (157-226). E-value: 1.6e-24. Score:
                     77.9. Matches aSDomain: nrpspksdomains_JYU28_RS25050_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (241-525). E-value:
                     1.7e-106. Score: 347.6. Matches aSDomain:
                     nrpspksdomains_JYU28_RS25050_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (694-1096). E-value:
                     6.8e-113. Score: 369.3. Matches aSDomain:
                     nrpspksdomains_JYU28_RS25050_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1203-1270). E-value: 2.4e-24.
                     Score: 77.3. Matches aSDomain:
                     nrpspksdomains_JYU28_RS25050_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (1287-1572). E-value:
                     4.5e-103. Score: 336.3. Matches aSDomain:
                     nrpspksdomains_JYU28_RS25050_Condensation_LCL.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="JYU28_RS25050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_25090"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_245231685.1"
                     /sec_met_domain="PP-binding (E-value: 2.8e-18, bitscore:
                     54.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.6e-91, bitscore:
                     294.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.9e-123, bitscore:
                     398.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRPELTEEKFAANPFAPGERMYRTGDLARWLPDGNLEYLGRIDH
                     QVKIRGYRIELGEIENRLLKHEAIKEAVVLDKEGADGNKYLCGYVVPDQEITVQGMREH
                     LSKELPDYMIPSYFVRMEKLPLTPNGKIDRKALPVLDSNVNTGVEYVVPSGEIEEKLAL
                     LWRDILGIEKVGRNDNFFMLGGHSLKATSLVARIHKEFNIDIPLKEIFKTPTILEIARY
                     IKDSKESLYQSIQPAGEMEYYPMSSAQRRLYILNQLEGAGTAYNMPGIMLLEGSIDTAR
                     FQEVFRQLVKRHETLRTSFHMIDGNPVQKVHDHVEFSVMYFETNEEKAAEYAGEFARPF
                     DLGKAPLLRVELVKTGEDRHLMMFDMHHIISDGVSMDILIREFMDLYEGNNPPALRIQY
                     KDYAVWQNKLFDEGGISSQEEYWLQAFQGEIPVLNMPLDYPRPVIQSFEGDFFSFEAGS
                     ELTNKLNKLARDNGATLYMTLLAAYNILLSKHTGQEDIIVGSPIAGRPHSDLQDILGMF
                     VNTLAMRNYPKGDSTFVEFLNEVRENALRAYDNQDYQFEELVEKLNLKRDMSRNPLFDT
                     MFTMQNASKVEFAVPGISFRPFNNGLEYRISKFDLSIDAIEREDGMAFSFEYCTRLFKK
                     ETLKRLAAHYLNVLEEITANPFKRLSEINMLSQEEKQQIIYGFNDTKVEYPKDKTIQEL
                     FEEHVEKTPDNIAVVFEDKYLTYGELNEKANQLAWILRDKGLKPDSVVAIMVDRSIEMI
                     IGVIAVFKAGGAYLPIDTEYPEERIRYMIDDSLAGIILTQEHHKEKIKFDLDIISFDDC
                     VNYNGRTENLPVINKSCNLAYVIYTSGTTGKPKGIMIEHRSLVNISYAWKKEYRLADMD
                     VKLLQIASFSFDVFVGDVSRTLLNGGQMVICSSEERYDPARLYSLIKTHKITIFESTPA
                     LIIPLMDYIHDNALIIDSLKLLILGSDTLSVEDYERLQSWYGEKLRVVNSYGVTESTID
                     SSYYEKRLGSYLESGSVPIGKPMQNIKYYVVDKHLRPQPTGVLGELCIGGEGLARGYLN
                     RPELTAEKFVPNPFIPGERMYRTGDLARWLSDGNMEFLGRVDHQVKIRGFRIELGEIEA
                     QLLKHISIKEAVVIAREDQKGNKYLCAYVAGENELTIAELRGHLLKDLPDYMIPSYFMQ
                     LKKLPLTPNGKIDRKALPEPDGNIDAGIEYVAPLGEVEEKLAGLWQDILSIEKVGRNDN
                     FFELGGHSLRAANLIARIHKEFNVNIPLKEIFKSPNMSEVAECIKIAKESVFHSIGPAG
                     EMEYYPISSAQKRLYILNQLDAVGMAYNMPGIMLIEGKLDKLRFEQAFRELVDRHESFR
                     TSFHMIDGKPVQKVHSNVDFSVMYMETDEERAAKIASEFIRTFDLDEAPLLRVGLIKVG
                     EDRYNMMFDKHHIISDGVSDDILVREFMSLYEGKELPELKIQYKDFSVWQNRLFADGTI
                     SKQEEYWLQVFKGEIPLLNMPLDYPRPAIQSFEGDVISFKAGKELAVKLNKLAVNNGAT
                     LYMVLLASYNVLLSKYTGQEDIIVGSPVAGRPHTDLQDVIGMFVNTLAMRNHPQGGKTF
                     VDFLNEVRENSLRAYENQDYQFEELVDKLNLERDLGRNPLFDTMFVLQNIEASEVKMDG
                     LRFMPYDSEVRISKFDLTLNAEETEDGIAFELEYATRLFKKESVIRLIAHYLNVLEEAA
                     AFPEKRLLEIDMLSQDEKHQILYEFNNTKAGYPREKTIHQIFEEQVERTPNNIAVVFED
                     KQLTYRELNEKANQLARKLREKGIKPDSVVGIMVERSPEMIIGIMAVLKA"
     CDS_motif       950..1015
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0001"
                     /evalue="1.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="60"
                     /protein_start="38"
                     /score="39.6"
                     /tool="antismash"
                     /translation="YLGRIDHQVKIRGYRIELGEIE"
     CDS_motif       1223..1249
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0002"
                     /evalue="9.80E-02"
                     /label="NRPS-A_a10"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="138"
                     /protein_start="129"
                     /score="5.7"
                     /tool="antismash"
                     /translation="GKIDRKALP"
     aSDomain        1307..1513
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS25050_PCP.1"
                     /evalue="1.60E-24"
                     /label="JYU28_RS25050_PCP.1"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="226"
                     /protein_start="157"
                     /score="77.9"
                     /tool="antismash"
                     /translation="EEKLALLWRDILGIEKVGRNDNFFMLGGHSLKATSLVARIHKEFN
                     IDIPLKEIFKTPTILEIARYIKDS"
     aSDomain        1559..2410
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS25050_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.70E-106"
                     /label="JYU28_RS25050_Condensation_LCL.1"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="525"
                     /protein_start="241"
                     /score="347.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="YYPMSSAQRRLYILNQLEGAGTAYNMPGIMLLEGSIDTARFQEVF
                     RQLVKRHETLRTSFHMIDGNPVQKVHDHVEFSVMYFETNEEKAAEYAGEFARPFDLGKA
                     PLLRVELVKTGEDRHLMMFDMHHIISDGVSMDILIREFMDLYEGNNPPALRIQYKDYAV
                     WQNKLFDEGGISSQEEYWLQAFQGEIPVLNMPLDYPRPVIQSFEGDFFSFEAGSELTNK
                     LNKLARDNGATLYMTLLAAYNILLSKHTGQEDIIVGSPIAGRPHSDLQDILGMFVNTLA
                     MRN"
     aSModule        1559..4645
                     /complete
                     /domains="nrpspksdomains_JYU28_RS25050_Condensation_LCL.1"
                     /domains="nrpspksdomains_JYU28_RS25050_AMP-binding.1"
                     /domains="nrpspksdomains_JYU28_RS25050_PCP.2"
                     /locus_tags="JYU28_RS25050"
                     /monomer_pairings="Orn -> Orn"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1622..1738
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0003"
                     /evalue="6.60E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="301"
                     /protein_start="262"
                     /score="50.7"
                     /tool="antismash"
                     /translation="TAYNMPGIMLLEGSIDTARFQEVFRQLVKRHETLRTSFH"
     CDS_motif       1931..1996
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0004"
                     /evalue="2.70E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="387"
                     /protein_start="365"
                     /score="32.7"
                     /tool="antismash"
                     /translation="MHHIISDGVSMDILIREFMDLY"
     CDS_motif       2027..2062
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0005"
                     /evalue="2.30E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="409"
                     /protein_start="397"
                     /score="16.9"
                     /tool="antismash"
                     /translation="QYKDYAVWQNKL"
     CDS_motif       2318..2407
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0006"
                     /evalue="3.50E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="524"
                     /protein_start="494"
                     /score="48.3"
                     /tool="antismash"
                     /translation="DIIVGSPIAGRPHSDLQDILGMFVNTLAMR"
     CDS_motif       2456..2575
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0007"
                     /evalue="3.00E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="580"
                     /protein_start="540"
                     /score="61.4"
                     /tool="antismash"
                     /translation="RENALRAYDNQDYQFEELVEKLNLKRDMSRNPLFDTMFTM"
     aSDomain        2918..4123
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS25050_AMP-binding.1"
                     /evalue="6.80E-113"
                     /label="JYU28_RS25050_AMP-binding.1"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1096"
                     /protein_start="694"
                     /score="369.3"
                     /specificity="substrate consensus: Orn"
                     /tool="antismash"
                     /translation="FEEHVEKTPDNIAVVFEDKYLTYGELNEKANQLAWILRDKGLKPD
                     SVVAIMVDRSIEMIIGVIAVFKAGGAYLPIDTEYPEERIRYMIDDSLAGIILTQEHHKE
                     KIKFDLDIISFDDCVNYNGRTENLPVINKSCNLAYVIYTSGTTGKPKGIMIEHRSLVNI
                     SYAWKKEYRLADMDVKLLQIASFSFDVFVGDVSRTLLNGGQMVICSSEERYDPARLYSL
                     IKTHKITIFESTPALIIPLMDYIHDNALIIDSLKLLILGSDTLSVEDYERLQSWYGEKL
                     RVVNSYGVTESTIDSSYYEKRLGSYLESGSVPIGKPMQNIKYYVVDKHLRPQPTGVLGE
                     LCIGGEGLARGYLNRPELTAEKFVPNPFIPGERMYRTGDLARWLSDGNMEFLGRVDHQV
                     KIR"
     CDS_motif       3107..3145
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0008"
                     /evalue="1.40E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="770"
                     /protein_start="757"
                     /score="15.2"
                     /tool="antismash"
                     /translation="AVFKAGGAYLPID"
     CDS_motif       3329..3388
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0009"
                     /evalue="8.00E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="851"
                     /protein_start="831"
                     /score="33.7"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGIMIEH"
     CDS_motif       3917..4006
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0010"
                     /evalue="1.20E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1057"
                     /protein_start="1027"
                     /score="55.9"
                     /tool="antismash"
                     /translation="PTGVLGELCIGGEGLARGYLNRPELTAEKF"
     CDS_motif       4088..4153
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0011"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1106"
                     /protein_start="1084"
                     /score="39.3"
                     /tool="antismash"
                     /translation="FLGRVDHQVKIRGFRIELGEIE"
     CDS_motif       4361..4387
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0012"
                     /evalue="9.80E-02"
                     /label="NRPS-A_a10"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1184"
                     /protein_start="1175"
                     /score="5.7"
                     /tool="antismash"
                     /translation="GKIDRKALP"
     aSDomain        4445..4645
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS25050_PCP.2"
                     /evalue="2.40E-24"
                     /label="JYU28_RS25050_PCP.2"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1270"
                     /protein_start="1203"
                     /score="77.3"
                     /tool="antismash"
                     /translation="EEKLAGLWQDILSIEKVGRNDNFFELGGHSLRAANLIARIHKEFN
                     VNIPLKEIFKSPNMSEVAECIK"
     aSDomain        4697..5551
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS25050_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.50E-103"
                     /label="JYU28_RS25050_Condensation_LCL.2"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1572"
                     /protein_start="1287"
                     /score="336.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="YYPISSAQKRLYILNQLDAVGMAYNMPGIMLIEGKLDKLRFEQAF
                     RELVDRHESFRTSFHMIDGKPVQKVHSNVDFSVMYMETDEERAAKIASEFIRTFDLDEA
                     PLLRVGLIKVGEDRYNMMFDKHHIISDGVSDDILVREFMSLYEGKELPELKIQYKDFSV
                     WQNRLFADGTISKQEEYWLQVFKGEIPLLNMPLDYPRPAIQSFEGDVISFKAGKELAVK
                     LNKLAVNNGATLYMVLLASYNVLLSKYTGQEDIIVGSPVAGRPHTDLQDVIGMFVNTLA
                     MRNH"
     CDS_motif       4763..4876
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0013"
                     /evalue="4.40E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1347"
                     /protein_start="1309"
                     /score="51.2"
                     /tool="antismash"
                     /translation="AYNMPGIMLIEGKLDKLRFEQAFRELVDRHESFRTSFH"
     CDS_motif       5072..5134
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0014"
                     /evalue="4.40E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1433"
                     /protein_start="1412"
                     /score="25.7"
                     /tool="antismash"
                     /translation="HHIISDGVSDDILVREFMSLY"
     CDS_motif       5165..5200
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0015"
                     /evalue="1.80E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1455"
                     /protein_start="1443"
                     /score="14.2"
                     /tool="antismash"
                     /translation="QYKDFSVWQNRL"
     CDS_motif       5456..5545
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0016"
                     /evalue="2.80E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1570"
                     /protein_start="1540"
                     /score="51.8"
                     /tool="antismash"
                     /translation="DIIVGSPVAGRPHTDLQDVIGMFVNTLAMR"
     CDS_motif       5594..5713
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS25050_0017"
                     /evalue="3.80E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="JYU28_RS25050"
                     /protein_end="1626"
                     /protein_start="1586"
                     /score="64.3"
                     /tool="antismash"
                     /translation="RENSLRAYENQDYQFEELVDKLNLERDLGRNPLFDTMFVL"
ORIGIN
        1 caaagtctgc ctgctgatac cgggaggaga aaaaaatccg gaagaaatcg tgaaggcaat
       61 cgaaaaaaat aaagttacca caatgcactt tgtaccatcc atgttgagtg tatttttaga
      121 gtatgtaaag gaaaagggga taatagagga aatagcaagc ttaaagcagg tatttgccag
      181 tggagaagca ctgaacctga agcaagtaga aaagttcaac aagctgctgt acaaagaaaa
      241 aggaacaagc ctgcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagaa
      361 gcactgaacc tgaagcaagt agaaaagttc aacaagctgc tgtacaaaga aaaaggaaca
      421 agcctgcata acctgtacgg accaacagaa gcaacagtag atgtatccta ctttggctgc
      481 tctacggaag aagaattgga gcttgtacct ataggaaagc ccatagataa cattaagttg
      541 tatgttgtca gtaaggaaaa gggcctgcag ccgataggag tagcgggaga actatgcata
      601 gcaggggatg gactggcaag agggtacttg aacagaccgg aacttacaga agaaaaattc
      661 gcagcaaacc cattcgcacc gggtgaaagg atgtacagga caggtgacct ggcaaggtgg
      721 ctgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaagag ggtacttgaa
      841 cagaccggaa cttacagaag aaaaattcgc agcaaaccca ttcgcaccgg gtgaaaggat
      901 gtacaggaca ggtgacctgg caaggtggct gcctgacggc aacctagaat atctgggcag
      961 gatcgaccat caggtaaaga taagaggcta caggatagaa ctgggtgaaa tagagaaccg
     1021 gttattaaag catgaagcaa taaaagaagc ggtggtattg gacaaagaag gagctgacgg
     1081 aaataagtat ctttgtggat acgtcgtacc ggatcaagaa ataaccgtac aggggatgag
     1141 ggaacacttg tcaaaagagc tcccggacta catgatccct tcctactttg tgcggatgga
     1201 aaagctgcca ttgaccccta atggaaaaat agacaggaaa gcattgcctg tgctcgatag
     1261 caatgttaat accggcgtag aatacgtagt accttcggga gaaatagagg agaaacttgc
     1321 gctgttgtgg cgggatatcc ttggaataga gaaggtagga agaaatgata acttttttat
     1381 gcttggggga cactcgttaa aggcaacaag ccttgtagca agaattcaca aagagttcaa
     1441 tatagacata cctcttaagg aaatattcaa gacaccaacc atcttagaaa ttgccagata
     1501 tattaaagac tcaaaggaga gcctatatca gtcaatccaa ccggcaggag aaatggaata
     1561 ctaccctatg tcatctgcac aaagaaggct gtatatacta aaccagcttg aaggggcagg
     1621 gacagcatac aacatgcctg gtattatgct gctggaagga agcattgata cagcgcgttt
     1681 ccaggaagtc ttcaggcagt tggtaaaaag gcatgagact cttagaacat cgttccacat
     1741 gatcgatggt aacccggtcc agaaggttca tgaccatgtg gaattcagcg tgatgtactt
     1801 tgaaactaac gaagaaaaag ccgcagagta tgctggggaa tttgccagac cttttgatct
     1861 tggcaaggct cctctgctaa gggtagagct tgtaaaaacg ggagaagaca ggcatcttat
     1921 gatgtttgat atgcatcaca ttatatccga tggtgtctcc atggacatac tgattcgtga
     1981 gtttatggat ctttatgaag gaaacaaccc tccagcactt cggatacagt acaaagacta
     2041 cgctgtatgg cagaataaac tgtttgatga gggcggaatt agcagccagg aggaatactg
     2101 gctccaagca ttccagggag aaatacctgt actgaatatg ccgctagact atccaaggcc
     2161 tgtaatacaa agctttgaag gggatttttt tagctttgaa gcaggcagtg agcttacgaa
     2221 taaattaaac aagcttgcaa gggataatgg ggctacgctt tacatgacac tgctcgcggc
     2281 atacaacata ttgctgtcta agcacaccgg acaggaagac ataatcgttg gaagccctat
     2341 agccggaagg ccacattcag acctgcagga tatccttggt atgtttgtaa ataccttggc
     2401 tatgagaaat taccccaagg gagatagtac ctttgtagaa ttcttgaatg aagtaagaga
     2461 aaacgctcta agggcttatg acaatcagga ttaccaattt gaagaactgg ttgagaagct
     2521 taacttgaag agggacatga gcagaaaccc actgtttgat actatgttta ccatgcaaaa
     2581 tgcctctaag gtagaatttg cagtacctgg tataagcttc agacctttta ataacgggct
     2641 agagtataga atatcaaaat ttgacctatc cattgatgca atagagcgtg aagatggtat
     2701 ggcttttagc tttgaatatt gtactcggct tttcaaaaaa gagactttaa aaaggttggc
     2761 ggctcattac cttaacgtgt tggaagagat aaccgcaaat ccgtttaaaa ggttgtcgga
     2821 aataaatatg ttgtcccagg aggaaaaaca gcaaataatc tatggcttca atgatacaaa
     2881 agtagaatat cctaaggata agaccataca agagctattt gaagaacacg tggaaaagac
     2941 gcctgataac atagcagtag tatttgaaga taaatacttg acttacggag aacttaatga
     3001 aaaggccaat caactggctt ggatcttaag ggacaaggga ttaaaacctg acagtgtagt
     3061 agctataatg gtagaccggt ccatagaaat gataataggt gtaattgctg tatttaaagc
     3121 aggaggcgcg tatttaccca tagacaccga atatcctgaa gaacgaatca gatatatgat
     3181 cgatgacagc ttggctggaa ttattcttac gcaagaacat cataaagaaa aaattaaatt
     3241 tgatcttgat attataagct ttgatgactg tgtgaattac aacgggagaa cagaaaatct
     3301 tcctgtcatt aataaatctt gtaatttagc atatgtcatt tatacatcag gaaccacagg
     3361 aaagccaaag gggatcatga tagagcatag aagtcttgtc aacatatctt acgcatggaa
     3421 aaaggaatat aggcttgcgg atatggatgt aaagctgttg caaatcgcaa gtttttcttt
     3481 tgatgtattt gtgggagatg tttcgagaac gctgctcaac ggaggccaaa tggttatttg
     3541 ttcatccgag gagagatacg atcctgcacg attatattca cttattaaaa cccataaaat
     3601 aaccatcttt gaatccacac cggcactgat tattcctctt atggattata tccatgataa
     3661 tgctttgatt attgatagcc tcaagttgct tatacttgga tcagatactc tctctgtgga
     3721 agattatgaa agattacaat cctggtatgg agagaaactg cgggttgtta atagctatgg
     3781 ggttactgag tccaccatag attccagtta ttacgaaaag agactgggaa gctatttgga
     3841 atccgggagt gtacctatcg gaaagccaat gcaaaatata aaatactacg ttgttgacaa
     3901 acacttgaga cctcagccta caggggtttt gggtgagttg tgcatagggg gagaagggct
     3961 tgcaagggga tatctaaacc ggcccgaact gacagctgaa aaatttgtac ctaacccgtt
     4021 tataccaggg gaaaggatgt atcgtacagg ggatctggcc agatggctgt ctgatgggaa
     4081 tatggaattt ttaggaagag tagatcatca ggtaaagata aggggattta gaatagagct
     4141 gggggaaata gaagcacaac tgctaaagca catttcaata aaggaagcag tggttattgc
     4201 aagggaagat cagaagggaa acaagtatct gtgtgcctat gtggcagggg aaaatgaatt
     4261 aacaatagca gaactaaggg gacatctctt aaaggacctt ccagactata tgataccatc
     4321 ctattttatg cagttaaaaa agctgccgct gactcccaat ggcaaaatag ataggaaggc
     4381 attgcccgaa cctgacggca atatcgatgc tggaatagag tatgtcgcac ccttgggtga
     4441 agtggaggag aaacttgcgg ggttgtggca ggatatcctt agtatagaga aagtgggaag
     4501 aaacgacaat ttctttgagc tggggggaca ttccttaagg gcagcaaatc ttattgccag
     4561 gattcacaaa gagttcaatg taaacatacc gcttaaagaa atatttaaga gcccaaatat
     4621 gtctgaagtt gccgagtgca tcaagattgc aaaggagagt gtatttcatt ctattgggcc
     4681 ggctggggag atggagtact atccaatttc atccgcccag aaaaggctgt atattctaaa
     4741 ccagcttgac gcagttggga tggcatacaa catgcctggt attatgctaa ttgaaggcaa
     4801 actcgataaa ctgcgctttg aacaggcttt tagggagctt gttgacagac atgagtcttt
     4861 ccgtacctca ttccatatga tagatggtaa accggtacaa aaggtccata gcaatgtgga
     4921 ttttagtgtt atgtatatgg aaaccgatga ggaaagagct gcgaaaatag ccagtgaatt
     4981 tattaggacc tttgaccttg atgaggctcc tttacttagg gttgggctta taaaagttgg
     5041 agaagacaga tataatatga tgtttgataa gcaccatatt atatctgacg gggtgtctga
     5101 cgacatactg gtaagagaat ttatgagcct gtatgaaggt aaggaacttc cggaacttaa
     5161 gattcagtac aaggattttt ccgtatggca gaacaggctg tttgctgatg ggacaattag
     5221 taagcaggaa gaatactggc tccaagtatt caaaggagaa attcctttgc ttaatatgcc
     5281 gctagactac cctaggccag ctatacagag ttttgaggga gatgtgatca gtttcaaagc
     5341 aggcaaggag cttgctgtta aactgaacaa acttgcagtg aataatggtg ccacgctcta
     5401 catggtactt ttagcttcat acaatgtcct tttatccaag tacaccggac aggaagatat
     5461 tatagttgga agtcccgttg caggaaggcc gcatactgat cttcaggacg ttattggtat
     5521 gtttgtaaac acgctagcca tgaggaatca ccctcaaggt ggaaagactt ttgtggactt
     5581 ccttaatgaa gtaagagaaa actccttaag ggcttacgaa aaccaagatt accaatttga
     5641 agaactggta gataagctta acctggaaag ggatctgggt agaaatccac ttttcgatac
     5701 tatgtttgta ctgcaaaata tcgaggcatc agaagtgaag atggatgggc taaggtttat
     5761 gccctatgac agtgaagtaa gaatatccaa gtttgacttg actttaaacg ctgaggaaac
     5821 agaggacggt attgcatttg aattggagta tgctaccagg ttgtttaaga aagagtcagt
     5881 tataaggctt attgcccatt accttaatgt attggaagag gcagcagcct ttcctgaaaa
     5941 aaggctatta gaaattgata tgctgtcaca agacgaaaaa catcaaatat tatatgaatt
     6001 taataataca aaagcaggat accctcggga aaagaccatc catcagattt ttgaggaaca
     6061 ggtggaacgg acacccaata atattgctgt agtgtttgaa gataaacagc tgacttatag
     6121 ggagctaaac gaaaaggcaa accaactggc cagaaaatta agagaaaagg gaataaaacc
     6181 tgatagtgta gtgggaatta tggtagaacg ttccccggaa atgatcatag ggatcatggc
     6241 tgtcctaaaa gc
//
