LOCUS       NZ_JAFJXZ010000006      4725 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Paenibacillus polymyxa strain Hob6 Scaffold_6, whole genome shotgun
            sequence.
ACCESSION   NZ_JAFJXZ010000006
VERSION     NZ_JAFJXZ010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Draft genome sequence of Paenibacillus polymyxa strain Hob6,
            isolated from hemp (Cannabis sative) seeds' oil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2021) Plant Science Departement, McGill
            University, 21111 Lakeshore, te-Anne-de-Bellevue, Quebec H9X 3V9,
            Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2020
            Assembly Method                   :: SPAdes v. 3.12.0; Medusa v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly :: GCA_000507205.2; GCA_014706575.1;
            GCA_000164985.2; GCA_000597985.1; GCA_002893885.1
            Genome Coverage                   :: 266.29x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017589695.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:57:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,119
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 156
            rRNAs                             :: 21, 9, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 19, 2 (5S, 16S)
            partial rRNAs                     :: 2, 7, 15 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 44 of 95
            Pseudo Genes (incomplete)         :: 63 of 95
            Pseudo Genes (internal stop)      :: 13 of 95
            Pseudo Genes (multiple problems)  :: 21 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4725
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAFJXZ010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4725
                     /collected_by="Mohannad Mahmoud, Suha Jabaji"
                     /collection_date="2019-11-18"
                     /db_xref="taxon:1406"
                     /host="Cannabis sativa"
                     /identified_by="Mohannad Mahmoud"
                     /isolation_source="commercial industrial hemp seed oil"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus polymyxa"
                     /strain="Hob6"
                     /submitter_seqid="Scaffold_6"
     region          1..4725
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..4725
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4725
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[782:>1497](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      783..>1497
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            41..790
                     /locus_tag="JYU28_RS26355"
     CDS             41..790
                     /NRPS_PKS="Domain: AMP-binding (3-213). E-value: 3.4e-52.
                     Score: 169.3. Matches aSDomain:
                     nrpspksdomains_JYU28_RS26355_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="JYU28_RS26355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_348537754.1"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-69, bitscore:
                     222.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVGDIARTLYNGGTMVICPKDDRIDFSRLHGWIRDVQITVFEST
                     PALIVPFMQHVHEQGLDMSSLELLITSSDSCSVTDYRVLQERFGADIRIMNSYGVTEAA
                     IDSSFYDEELSKLPSSGHVPIGQAWLNARFYIVDSQLNPVPIGVLGELCIGGAGVARGY
                     LNRADLTAEKFVANPYVPGERLYRTGDLARWMPDGNVDFIGRMDDQVKIRGFRIELGEV
                     EAQLLTVDGIQKAIVTAWENGLYRPDG"
     aSDomain        50..679
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS26355_AMP-binding.1"
                     /evalue="3.40E-52"
                     /label="JYU28_RS26355_AMP-binding.1"
                     /locus_tag="JYU28_RS26355"
                     /protein_end="213"
                     /protein_start="3"
                     /score="169.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="GDIARTLYNGGTMVICPKDDRIDFSRLHGWIRDVQITVFESTPAL
                     IVPFMQHVHEQGLDMSSLELLITSSDSCSVTDYRVLQERFGADIRIMNSYGVTEAAIDS
                     SFYDEELSKLPSSGHVPIGQAWLNARFYIVDSQLNPVPIGVLGELCIGGAGVARGYLNR
                     ADLTAEKFVANPYVPGERLYRTGDLARWMPDGNVDFIGRMDDQVKIR"
     misc_feature    complement(51..61)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     CDS_motif       473..562
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS26355_0001"
                     /evalue="1.10E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="JYU28_RS26355"
                     /protein_end="174"
                     /protein_start="144"
                     /score="59.2"
                     /tool="antismash"
                     /translation="PIGVLGELCIGGAGVARGYLNRADLTAEKF"
     CDS_motif       644..709
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS26355_0002"
                     /evalue="1.40E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="JYU28_RS26355"
                     /protein_end="223"
                     /protein_start="201"
                     /score="42.5"
                     /tool="antismash"
                     /translation="FIGRMDDQVKIRGFRIELGEVE"
     CDS_motif       783..836
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS26360_0001"
                     /evalue="2.30E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="JYU28_RS26360"
                     /protein_end="18"
                     /protein_start="0"
                     /score="32.5"
                     /tool="antismash"
                     /translation="MDDQVKIRGFRIELGEVE"
     gene            783..>1497
                     /locus_tag="JYU28_RS26360"
     CDS             783..>1497
                     /NRPS_PKS="Domain: PCP (115-184). E-value: 6.9e-27. Score:
                     85.5. Matches aSDomain: nrpspksdomains_JYU28_RS26360_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /locus_tag="JYU28_RS26360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_245231530.1"
                     /sec_met_domain="AMP-binding (E-value: 1.9e-10, bitscore:
                     27.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.5e-19, bitscore:
                     56.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.6e-09, bitscore:
                     23.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDQVKIRGFRIELGEVEAQLLTVDGIQKAIVTAWENEDGDKDLC
                     AYVVASEALNLPDLRSTLQPKLPGYMIPTYVVQLDRFPLTPNGKIDRKALPAPEARLEG
                     GTAYVAPRTPLEEQLVRIWQSVLNHHQIGIQDNFFEVGGHSLRATTLMAKIHQELNHKL
                     ALRDIFQYPTIEQLVQLMGEDQTQQTYVSIPVVEEREYYPVSSAQKRMYVLSHLEGGEV
                     SYNMPGALIIEGSLDK"
     CDS_motif       1044..1070
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS26360_0002"
                     /evalue="6.70E-03"
                     /label="NRPS-A_a10"
                     /locus_tag="JYU28_RS26360"
                     /protein_end="96"
                     /protein_start="87"
                     /score="8.8"
                     /tool="antismash"
                     /translation="GKIDRKALP"
     aSDomain        1128..1334
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS26360_PCP.1"
                     /evalue="6.90E-27"
                     /label="JYU28_RS26360_PCP.1"
                     /locus_tag="JYU28_RS26360"
                     /protein_end="184"
                     /protein_start="115"
                     /score="85.5"
                     /tool="antismash"
                     /translation="EEQLVRIWQSVLNHHQIGIQDNFFEVGGHSLRATTLMAKIHQELN
                     HKLALRDIFQYPTIEQLVQLMGED"
     assembly_gap    1498..1597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     gene            <1598..>4369
                     /locus_tag="JYU28_RS02970"
                     /old_locus_tag="JYU28_02985"
                     /pseudo=""
     CDS             1600..>4369
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: AMP-binding (2-226). E-value: 5.6e-55.
                     Score: 178.5. Matches aSDomain:
                     nrpspksdomains_JYU28_RS02970_AMP-binding.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310169.1"
                     /locus_tag="JYU28_RS02970"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_02985"
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.2e-72, bitscore:
                     232.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRLLQLASFSFDVFVGDIARTLYNGGTMVICPKDDRIDFSRLYG
                     WIRDVQITVFESTPALIVPFMQHVHEQGLDMSSLELLITSSDSCSVTDYRVLQERFGAD
                     IRIMNSYGVTEAAIDSSFYDEELSKLPSSGHVPIGQAWLNARFYIVDSQLNPVPIGVLG
                     ELCIGGAGVARGYLNRADLTAEKFVANPYVAGERLYRTGDLARWMPDGNVDFIGRMDDQ
                     VKIRGFRIELGEVEAQLLTVDGIQKAIVTAWENGGA"
     aSDomain        1607..2278
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02970_AMP-binding.1"
                     /evalue="5.60E-55"
                     /label="JYU28_RS02970_AMP-binding.1"
                     /locus_tag="JYU28_RS02970"
                     /protein_end="226"
                     /protein_start="2"
                     /score="178.5"
                     /specificity="substrate consensus: Dab"
                     /tool="antismash"
                     /translation="RLLQLASFSFDVFVGDIARTLYNGGTMVICPKDDRIDFSRLYGWI
                     RDVQITVFESTPALIVPFMQHVHEQGLDMSSLELLITSSDSCSVTDYRVLQERFGADIR
                     IMNSYGVTEAAIDSSFYDEELSKLPSSGHVPIGQAWLNARFYIVDSQLNPVPIGVLGEL
                     CIGGAGVARGYLNRADLTAEKFVANPYVAGERLYRTGDLARWMPDGNVDFIGRMDDQVK
                     IR"
     misc_feature    complement(1648..1658)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     CDS_motif       2072..2161
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02970_0001"
                     /evalue="1.10E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="JYU28_RS02970"
                     /protein_end="187"
                     /protein_start="157"
                     /score="59.2"
                     /tool="antismash"
                     /translation="PIGVLGELCIGGAGVARGYLNRADLTAEKF"
     CDS_motif       2243..2308
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02970_0002"
                     /evalue="1.40E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="JYU28_RS02970"
                     /protein_end="236"
                     /protein_start="214"
                     /score="42.5"
                     /tool="antismash"
                     /translation="FIGRMDDQVKIRGFRIELGEVE"
     assembly_gap    4370..4469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     gene            <4470..>4725
                     /locus_tag="JYU28_RS27070"
                     /old_locus_tag="JYU28_02990"
     CDS             4470..>4725
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="JYU28_RS27070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_02990"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_208332445.1"
                     /sec_met_domain="Condensation (E-value: 5.9e-25, bitscore:
                     76.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEREYYPVSSAQKRMYVLSHLEGGEVSYNMPGALIIEGSLDKKRL
                     EQAFRQLIARHEALRTRFEMVDGEVIQRIESGVAFSVEHV"
ORIGIN
        1 cccagtccga ctgctgcaac tggcgagctt ctccttcgac gtgttcgtcg gggacattgc
       61 acggacgctc tataacggcg gcacgatggt catttgtccg aaagacgacc ggattgattt
      121 tagccgtctg cacggatgga ttcgcgatgt ccagattacc gtgttcgaat cgactccggc
      181 cctgatcgta ccgtttatgc agcatgtgca tgaacagggg ctggacatga gcagtctgga
      241 gctgctgatc acgagctcgg atagttgcag cgtgacggac taccgagtgc tgcaagagcg
      301 gtttggtgcg gatatccgca ttatgaacag ctacggcgtc accgaagcgg cgattgactc
      361 cagcttctac gacgaagagc tgtcgaagct gccaagcagc ggccatgtgc cgattggtca
      421 ggcgtggctg aacgcacgct tctacattgt ggacagccag ctgaatccgg tgccgatcgg
      481 cgtactgggt gagctgtgca ttggcggagc cggggtggca cgaggctacc tgaatcgtgc
      541 agacctgaca gccgagaaat tcgtagcgaa tccgtatgta ccaggtgaac ggttgtaccg
      601 tacaggcgat ttggcgcgat ggatgccgga cggcaatgta gactttatcg gccggatgga
      661 tgatcaagtc aaaatccggg gcttccggat tgaactgggc gaggtggaag cgcagctgct
      721 gaccgtggac ggcattcaga aagccatcgt caccgcctgg gaaaacggac tttatcggcc
      781 ggatggatga tcaagtcaaa atccggggct tccggattga actgggcgag gtggaagcgc
      841 agctgctgac cgtggacggc attcagaaag ccatcgtcac cgcctgggaa aacgaagacg
      901 gagacaagga tctgtgtgcg tatgtcgtag cctcggaggc gctgaatttg ccggatctgc
      961 gcagcacact ccagcccaaa ctgccgggct atatgatccc gacctatgtc gtacaactgg
     1021 atcgtttccc gctgacaccg aacggaaaaa ttgaccgcaa agcgttgcct gcgccagaag
     1081 cgcgcctcga aggaggcacg gcgtacgtgg caccgcgcac accgctggaa gagcagttgg
     1141 tacgtatttg gcaaagcgtt ctgaatcatc atcaaatagg catccaggat aacttctttg
     1201 aagtgggagg acattctctg cgcgctacca ccctgatggc gaaaatccat caggagctga
     1261 atcacaagct ggcgttgcga gatattttcc aatatccgac gattgagcaa ctggtgcagt
     1321 tgatgggtga agatcagacc cagcaaacgt atgtatctat tcctgtggtg gaggagcgtg
     1381 aatattatcc tgtttcctcg gcgcaaaaac ggatgtacgt gctgagtcac ctggaaggtg
     1441 gagaggttag ctacaacatg ccaggagcgt tgattatcga aggttccttg gataaaannn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc agtccgactg ctgcaactgg
     1621 cgagcttctc cttcgacgtg ttcgtcgggg acattgcacg gacgctctat aacggcggca
     1681 cgatggtcat ttgtccgaaa gacgaccgga ttgattttag ccgtttgtat ggctggattc
     1741 gcgatgtcca gataaccgtg ttcgaatcga ctccagccct gatcgtaccg tttatgcagc
     1801 atgtgcatga acaggggctg gacatgagca gtctggaact actaatcacc agttcggaca
     1861 gctgcagtgt gacggactac cgggtgctgc aagaacggtt tggtgcggat atccgcatta
     1921 tgaacagcta cggcgtcacc gaagcggcga ttgactccag cttctacgac gaagagctgt
     1981 caaagctgcc aagcagcggc catgtgccga ttggtcaggc gtggctgaac gcacgcttct
     2041 acattgtgga cagccagctg aatccggtgc cgatcggcgt actgggtgag ctgtgcattg
     2101 gtggagccgg ggtggcacgg ggatatctga atcgtgcaga cctgacggcc gaaaaattcg
     2161 tagcgaatcc atatgtagcg ggtgaacgat tgtaccgtac aggcgatttg gcgcggtgga
     2221 tgccggatgg caatgtggac tttatcggcc ggatggatga tcaagtcaaa atccggggct
     2281 tccggattga actgggcgag gtggaagcgc agctgctgac cgtggacggc attcagaaag
     2341 ccatcgtcac cgcctgggaa aacggaggag cgtgaatatt atcctgtttc ctcggcgcaa
     2401 aaacggatgt acgtgctgag tcacctggaa ggtggagagg ttagctacaa catgccagga
     2461 gcgttgatta tcgaaggttc cttggataaa atgcgactgg aacaagcctt ccgccagctg
     2521 attgcccgtc atgaagccct gcgaacccga ttcgagatgg tggatggtga ggtgattcaa
     2581 cggattgaat cgggcgtcgc attcagcgtg gagcatgtgc aggcaagcga agaagagacc
     2641 cagcagtatg cccaagactt tgtacgcgca tttgatctcg cacaagcacc attgctgcgg
     2701 gttcagctgg tggaaaccgg accggagcgt cacgtcatgc tctatgacat gcaccatatt
     2761 atttcggatg gcgtcacgga aggaattatc gtgcaggagt tctctcaatt gtatgaaggc
     2821 aaggaactgc cgccactccg tattcagtac aaggactatg cggtttggca gcatgcagat
     2881 atgcaaagtg agcggcttcg tgagcaggaa agctactggc tgaacacgat ggctggtgac
     2941 attccggtgc tggatatgcc tacggatttt gcaagaccag ccgtacagcg tctggaagga
     3001 gctcgattgg aatttgcgat cagcctggag gacagcgaac agttgaaaca gctgtccgcg
     3061 caaaccggtt ccaccttgta catggtgttg ctggccgcgt atagcacact cttgcataaa
     3121 tacacgggtc aagaggacat tatcgtcggc acgccaattg ccggaagacc acatgcggaa
     3181 ctggagagct tggtaggcgt attcctcaac acgctggcaa tccgcaatta tccggctggt
     3241 gacaaaacgt tcctggacta cttgcaagag gtcaaagagc atgcgctcag tgcttacgaa
     3301 catcaggaat atccttttga agagctggtt gaaaagctga atttaacgcg ggatacgagc
     3361 cggaatgcgc tgtttgacac gatgtttgag ttgaaaacat ttgagcagca ggagttcgag
     3421 ctggaaggtc tgacctttaa gacgtatccg acagacaatc atacggccaa atttgatctg
     3481 accttggatg ccgtggagca gccggaaggc attctgtgca gtctggaata cagcacggcg
     3541 ctgtacaagc ctgagaccat tgcgcgcttg gcacagcatt ttaccgaact tgtacgtgcg
     3601 attactcttc atccgcagca atcactggca gctttggaaa tggtaaccgt cgaggaaaaa
     3661 gcacaaatca tctacggttt tggagatgtg ggagtatcgg atgttactgc tgaagcagga
     3721 ggcttattcc atacttatgt ggaagaacag acccagctta taccagatca tgtggcggtg
     3781 gtgtacgagg aacaacaact gacgtaccgc acactgaacg agcgtgcgaa ccaattagcc
     3841 cgtagactgc gaaatgaagg cattggacgc gagtcgattg ttggcatttt gagtgaacgt
     3901 tcggtcgaca tgctggttgg cgtactggcg gtgtggaagg caggaggcgc ctatgtgccg
     3961 ctggatgcag attatccgtc cgaacgtatt cgtttcatgc tggaggacag tggtgcgacg
     4021 gtactgttga cccaaatcgg cttgcaggag cgtgcacagg catggctcga ggaaagccaa
     4081 cctacatccg aacccgcaac agggctgcgt ctgcacaccg tactggcact ggacgatgag
     4141 tcgctgtaca ccggagatac cacagacgtc aatcacatca acgaaccgca ggatctggcg
     4201 tatgtgatct acacgtccgg tactacagga cgcccgaaag gggtcatgat cgagcatcgc
     4261 agcttggtga ataccgccgc ggcgtatcgt cgggattatc gcctgaacca gttcccggta
     4321 cgtttgctgc agctggcaag cttctccttc gacgtgttcg tcggcgatan nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aggagcgtga atattatcct gtttcctcgg
     4501 cgcaaaaacg gatgtacgtg ctgagtcacc tggaaggtgg agaggttagc tacaacatgc
     4561 caggagcgtt gattatcgaa ggttccttgg ataaaaaacg actggaacaa gccttccgcc
     4621 agctgattgc ccgtcatgaa gccctgcgaa cccgattcga gatggtggat ggtgaggtga
     4681 ttcaacggat tgaatcgggc gtcgcattca gcgtggagca tgtgc
//
