LOCUS       NZ_JAFJXZ010000005      8895 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Paenibacillus polymyxa strain Hob6 Scaffold_5, whole genome shotgun
            sequence.
ACCESSION   NZ_JAFJXZ010000005
VERSION     NZ_JAFJXZ010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Draft genome sequence of Paenibacillus polymyxa strain Hob6,
            isolated from hemp (Cannabis sative) seeds' oil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Mahmoud,M. and Jabaji,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2021) Plant Science Departement, McGill
            University, 21111 Lakeshore, te-Anne-de-Bellevue, Quebec H9X 3V9,
            Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-JUL-2020
            Assembly Method                   :: SPAdes v. 3.12.0; Medusa v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly :: GCA_000507205.2; GCA_014706575.1;
            GCA_000164985.2; GCA_000597985.1; GCA_002893885.1
            Genome Coverage                   :: 266.29x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017589695.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:57:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,119
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 156
            rRNAs                             :: 21, 9, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 19, 2 (5S, 16S)
            partial rRNAs                     :: 2, 7, 15 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 44 of 95
            Pseudo Genes (incomplete)         :: 63 of 95
            Pseudo Genes (internal stop)      :: 13 of 95
            Pseudo Genes (multiple problems)  :: 21 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8895
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAFJXZ010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8895
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:7496](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..7496)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..4135)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (32-98). E-value: 1.8e-26. Score:
                     84.1. Matches aSDomain: nrpspksdomains_JYU28_RS02950_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (109-412). E-value:
                     3.5e-156. Score: 513.0. Matches aSDomain:
                     nrpspksdomains_JYU28_RS02950_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (416-568). E-value: 1.2e-53.
                     Score: 173.0. Matches aSDomain:
                     nrpspksdomains_JYU28_RS02950_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (578-877). E-value:
                     4.5e-110. Score: 359.4. Matches aSDomain:
                     nrpspksdomains_JYU28_RS02950_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1159-1376). E-value:
                     8.5e-56. Score: 181.2. Matches aSDomain:
                     nrpspksdomains_JYU28_RS02950_AMP-binding.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281409.1"
                     /locus_tag="JYU28_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_02965"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_245231524.1"
                     /sec_met_domain="PP-binding (E-value: 2.2e-15, bitscore:
                     45.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2e-78, bitscore:
                     251.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 5.6e-62, bitscore:
                     197.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSNGKIDRKALPEPEGSIHTGAAYVAPRTTVEQVLAAVWAGVLG
                     VEAVGTQDNFFELGGDSIKALQVSSRLLQAGYRLEMKDLFSHPTVSALALKVQSVTRLA
                     DQSEVVGAVKLTPVQQWFFEQNLTDAHHHNQSIMLHSKDGFDEPALRTAMDQIVSHHDA
                     LRIIFRPTERGYEAWNRAVGEGELYTLDRADFRNESEVSAAIEAKVNEIQAGIHLSEGP
                     LVKLGLFHCPDGDHLLIAIHHLVVDTVSWRILFEDITTAYEQALNGQAARLPHKTDSFR
                     TWADGQSQYANSPAMAAELAFWQEMEQGVYQPLPKDESPSHSLNRDSENITVTWTAQET
                     EQLLKQAHRAYNTEMNDLLLTALGLTIHRWAGLERMLVSLEGHGRESILPELDITRTVG
                     WFTTQFPVVLDMSVGQDLSQRIKQVKEGLRRIPQKGIGYGMLRYLSAAREQEHFVTEPE
                     ISFNYLGQFDQDMQNQSIGVSPYASGAELSAHAARTHTLDMNGLISEGKLQLTISYSKQ
                     EYRRETMEQLAEQFRISLQEIITHCTSRKQQELTPSDVMLKHVTMAELDQLVERTRDMG
                     ELENVYALTPMQKGMLFHSLMDAESGAYFEQTTFDLHGRFQADIFQDSLNHLAQRHEIF
                     RANFISGWQDEPVQVIFRRKDVGFRCEDLRHLNEQERDAYVKEFIRKDKAAGFDLSRDA
                     LMRVAILRTGDEDYYFVWSSHHILMDGWCVALVTDEVFEAYFAAVEHRQPKLAPVTPYS
                     QYIEWLEAQDVEAAASYWKDYLLGYDQQTSIPTGKVGVKADGAQFEHILCDLGKDLTGR
                     MEMVAKQHHVTANTLLQTAWGLLLQKYNGSDDAVFGGVVSGRPADITGIENMVGLFINT
                     IPIRIRSGAEDTFADMIRTNQQQALTSQDYETYPLYEIQALSEQKQDLINHIIVFENYP
                     VEEQVEQLGEGAASDFEIRNAGMIEQTNYDFNLVVMPQKAMKIRFEYNAKLYDREAVER
                     TQGHLIQLLEQVVAKPDIRVHELDMVSEQEREQILSVWGNTAAEYPSGQTIHGLFEAQT
                     KQTPEQPALFFEGEQLTYRELNERANRLARTLRSQGVTKDRLVGLMTERSVDMIVGIFG
                     ILKAGGAYVPIDPTYPEERIRYMLDDSGAKLLLTQNHLVDKVAFDGSVLVLINEKANQL
                     ARKLREKGIKPDSVVGIMVERSPEMIIGIMAVLKAGGAYLPIAPEYPEDRIQFMLDDSG
                     SQIILIQDKFINAKGSYRNAEFISIENQELFTGDVSNPKLISKPENLAYVIYTSGSTGK
                     PKGVMIENYSVINRINWMQKQYPIAEGDVILQKTPYTFDVSVWELLWWSFTGAKVCLLI
                     PGGEKNPEEIVKAIEKNKVTTMHFVPSMLSVFLEYV"
     gene            complement(<1..>4139)
                     /locus_tag="JYU28_RS02950"
                     /old_locus_tag="JYU28_02965"
     source          1..8895
                     /collected_by="Mohannad Mahmoud, Suha Jabaji"
                     /collection_date="2019-11-18"
                     /db_xref="taxon:1406"
                     /host="Cannabis sativa"
                     /identified_by="Mohannad Mahmoud"
                     /isolation_source="commercial industrial hemp seed oil"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus polymyxa"
                     /strain="Hob6"
                     /submitter_seqid="Scaffold_5"
     region          1..8895
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8895
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="neighbouring"
                     /product="NRPS"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..8895
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[8041:>8891](-)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(8042..>8891)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
     aSDomain        complement(6..656)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02950_AMP-binding.1"
                     /evalue="8.50E-56"
                     /label="JYU28_RS02950_AMP-binding.1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="1376"
                     /protein_start="1159"
                     /score="181.2"
                     /specificity="substrate consensus: Ser"
                     /tool="antismash"
                     /translation="NEKANQLARKLREKGIKPDSVVGIMVERSPEMIIGIMAVLKAGGA
                     YLPIAPEYPEDRIQFMLDDSGSQIILIQDKFINAKGSYRNAEFISIENQELFTGDVSNP
                     KLISKPENLAYVIYTSGSTGKPKGVMIENYSVINRINWMQKQYPIAEGDVILQKTPYTF
                     DVSVWELLWWSFTGAKVCLLIPGGEKNPEEIVKAIEKNKVTTMHFVPSMLSVFL"
     aSModule        complement(6..2399)
                     /domains="nrpspksdomains_JYU28_RS02950_Condensation_DCL.1"
                     /domains="nrpspksdomains_JYU28_RS02950_AMP-binding.1"
                     /incomplete
                     /locus_tags="JYU28_RS02950"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(261..317)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0015"
                     /evalue="9.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="1291"
                     /protein_start="1272"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMIE"
     CDS_motif       complement(510..548)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0014"
                     /evalue="2.10E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="1208"
                     /protein_start="1195"
                     /score="14.7"
                     /tool="antismash"
                     /translation="MAVLKAGGAYLPI"
     CDS_motif       complement(777..818)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0013"
                     /evalue="1.20E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="1119"
                     /protein_start="1105"
                     /score="18.4"
                     /tool="antismash"
                     /translation="FGILKAGGAYVPID"
     CDS_motif       complement(1344..1460)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0012"
                     /evalue="3.90E-18"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="930"
                     /protein_start="891"
                     /score="57.9"
                     /tool="antismash"
                     /translation="QQQALTSQDYETYPLYEIQALSEQKQDLINHIIVFENYP"
     aSDomain        complement(1503..2399)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02950_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.50E-110"
                     /label="JYU28_RS02950_Condensation_DCL.1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="877"
                     /protein_start="578"
                     /score="359.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILMDG"
                     /tool="antismash"
                     /translation="ENVYALTPMQKGMLFHSLMDAESGAYFEQTTFDLHGRFQADIFQD
                     SLNHLAQRHEIFRANFISGWQDEPVQVIFRRKDVGFRCEDLRHLNEQERDAYVKEFIRK
                     DKAAGFDLSRDALMRVAILRTGDEDYYFVWSSHHILMDGWCVALVTDEVFEAYFAAVEH
                     RQPKLAPVTPYSQYIEWLEAQDVEAAASYWKDYLLGYDQQTSIPTGKVGVKADGAQFEH
                     ILCDLGKDLTGRMEMVAKQHHVTANTLLQTAWGLLLQKYNGSDDAVFGGVVSGRPADIT
                     GIENMVGLFINTIPIRIR"
     CDS_motif       complement(1509..1604)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0011"
                     /evalue="7.00E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="875"
                     /protein_start="843"
                     /score="57.0"
                     /tool="antismash"
                     /translation="DAVFGGVVSGRPADITGIENMVGLFINTIPIR"
     CDS_motif       complement(1845..1883)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0010"
                     /evalue="4.40E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="763"
                     /protein_start="750"
                     /score="9.9"
                     /tool="antismash"
                     /translation="PYSQYIEWLEAQD"
     CDS_motif       complement(1929..1991)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0009"
                     /evalue="6.70E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="735"
                     /protein_start="714"
                     /score="34.6"
                     /tool="antismash"
                     /translation="HHILMDGWCVALVTDEVFEAY"
     CDS_motif       complement(2214..2330)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0008"
                     /evalue="4.60E-15"
                     /label="C2_DCL_024-062"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="640"
                     /protein_start="601"
                     /score="47.9"
                     /tool="antismash"
                     /translation="GAYFEQTTFDLHGRFQADIFQDSLNHLAQRHEIFRANFI"
     CDS_motif       complement(2349..2390)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0007"
                     /evalue="5.20E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="595"
                     /protein_start="581"
                     /score="15.2"
                     /tool="antismash"
                     /translation="YALTPMQKGMLFHS"
     aSDomain        complement(2430..2885)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02950_TIGR01720.1"
                     /evalue="1.20E-53"
                     /label="JYU28_RS02950_TIGR01720.1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="568"
                     /protein_start="416"
                     /score="173.0"
                     /tool="antismash"
                     /translation="LSQRIKQVKEGLRRIPQKGIGYGMLRYLSAAREQEHFVTEPEISF
                     NYLGQFDQDMQNQSIGVSPYASGAELSAHAARTHTLDMNGLISEGKLQLTISYSKQEYR
                     RETMEQLAEQFRISLQEIITHCTSRKQQELTPSDVMLKHVTMAELDQL"
     aSModule        complement(2430..4037)
                     /domains="nrpspksdomains_JYU28_RS02950_PCP.1"
                     /domains="nrpspksdomains_JYU28_RS02950_Epimerization.1"
                     /domains="nrpspksdomains_JYU28_RS02950_TIGR01720.1"
                     /incomplete
                     /locus_tags="JYU28_RS02950"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(2736..2765)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0006"
                     /evalue="4.50E-03"
                     /label="NRPS-E7"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="466"
                     /protein_start="456"
                     /score="9.6"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(2802..2864)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0005"
                     /evalue="7.30E-11"
                     /label="NRPS-E6"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="444"
                     /protein_start="423"
                     /score="34.2"
                     /tool="antismash"
                     /translation="VKEGLRRIPQKGIGYGMLRYL"
     aSDomain        complement(2898..3806)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02950_Epimerization.1"
                     /evalue="3.50E-156"
                     /label="JYU28_RS02950_Epimerization.1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="412"
                     /protein_start="109"
                     /score="513.0"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDT"
                     /tool="antismash"
                     /translation="VGAVKLTPVQQWFFEQNLTDAHHHNQSIMLHSKDGFDEPALRTAM
                     DQIVSHHDALRIIFRPTERGYEAWNRAVGEGELYTLDRADFRNESEVSAAIEAKVNEIQ
                     AGIHLSEGPLVKLGLFHCPDGDHLLIAIHHLVVDTVSWRILFEDITTAYEQALNGQAAR
                     LPHKTDSFRTWADGQSQYANSPAMAAELAFWQEMEQGVYQPLPKDESPSHSLNRDSENI
                     TVTWTAQETEQLLKQAHRAYNTEMNDLLLTALGLTIHRWAGLERMLVSLEGHGRESILP
                     ELDITRTVGWFTTQFPVVLDMS"
     CDS_motif       complement(2907..2957)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0004"
                     /evalue="4.40E-08"
                     /label="NRPS-E5"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="409"
                     /protein_start="392"
                     /score="25.2"
                     /tool="antismash"
                     /translation="DITRTVGWFTTQFPVVL"
     CDS_motif       complement(3360..3425)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0003"
                     /evalue="9.40E-11"
                     /label="NRPS-E2"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="258"
                     /protein_start="236"
                     /score="33.9"
                     /tool="antismash"
                     /translation="LLIAIHHLVVDTVSWRILFEDI"
     CDS_motif       complement(3627..3701)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0002"
                     /evalue="2.30E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="169"
                     /protein_start="144"
                     /score="16.8"
                     /tool="antismash"
                     /translation="FDEPALRTAMDQIVSHHDALRIIFR"
     CDS_motif       complement(3762..3791)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02950_0001"
                     /evalue="4.70E-04"
                     /label="NRPS-E1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="124"
                     /protein_start="114"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTPVQQWFFE"
     aSDomain        complement(3840..4037)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02950_PCP.1"
                     /evalue="1.80E-26"
                     /label="JYU28_RS02950_PCP.1"
                     /locus_tag="JYU28_RS02950"
                     /protein_end="98"
                     /protein_start="32"
                     /score="84.1"
                     /tool="antismash"
                     /translation="EQVLAAVWAGVLGVEAVGTQDNFFELGGDSIKALQVSSRLLQAGY
                     RLEMKDLFSHPTVSALALKVQ"
     assembly_gap    4140..4239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     CDS             complement(<4240..7496)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (37-340). E-value:
                     8.6e-159. Score: 521.7. Matches aSDomain:
                     nrpspksdomains_JYU28_RS27065_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (344-496). E-value: 4.3e-54.
                     Score: 174.5. Matches aSDomain:
                     nrpspksdomains_JYU28_RS27065_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (506-805). E-value:
                     4.9e-111. Score: 362.6. Matches aSDomain:
                     nrpspksdomains_JYU28_RS27065_Condensation_DCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281409.1"
                     /locus_tag="JYU28_RS27065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_02970"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_208332443.1"
                     /sec_met_domain="Condensation (E-value: 4.2e-79, bitscore:
                     253.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.7e-33, bitscore:
                     102.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQAGYRLEMKDLFSHPTVSALALKVQSVTRLADQSEVVGAVKLTP
                     VQHWFFEQNLTDAHHHNQSIMLHSKDGFDEPALRTAMDQIVSHHDALRIVFRPTEHGYE
                     AWNRAVGEGELYTLERIDLSHETEVSAAIEAKVNEIQAGIQLSEGPLVKLGLFHCPDGD
                     HLLMAIHHLVVDTVSWRILFEDITTAYEQALNGQTARLPHKTDSFRTWADGQSQYANSP
                     AMAAELEFWKEMEHGVYQPLPKDESPSHSLNRDSENMTVTWTAQETEQLLKQAHRAYNT
                     EMNDLLLTALGLTIHRWAGLERVLVSLEGHGRESILPELDITRTVGWFTTQFPVVLDMS
                     VGQDLSQRIKQVKEGLRRIPQKGIGYGMLRYLSAAREQERFVTEPEISFNYLGQFDQDM
                     QSQSIGVSPYSSGAELSAHAARSHTLDMNGLISEGELQLTISYSKQEYRHETMEQLAEE
                     FKISLQEIIAHCVGRKQQELTPSDVMLKHVTMAELDQLVERTRDIGELENVYALTPMQK
                     GMLFHSLMDAASGAYFEQTTFDLDGRFQADIFQDSLNHLAQRHEIFRANFISGWQDEPV
                     QVIFRRKEVGFRCEDLRHLNEQERDAYVQEFIRQDKAAGFDLSRDALMRVAILRTGDED
                     YYFVWSSHHILMDGWCVALVTDEVFEAYFAAVEHRQPKLAPVTPYSQYIEWLEAQDEEA
                     AASYWKDYLLGYEQQTSIPTGKVGAKADGAQFEHILCDLGKDLTGRMEMVAKQHHVTPN
                     TLLQTAWGLLLQKYNGSDDAVFGGVVSGRPAEISGIENMVGLFINTIPIRIRSGADDTF
                     ADLIRTNQKQALTSQDYETYPLYEIQALTEQKQDLINHIIVFENYPVEEQVEQLGEGAA
                     SDFEIRNAGMIEQTNYDFNLVVMPQEAMKIRFEYNAKLYDREAVERTQGHLIQLLEQVV
                     AKPDIRVQELDMVSEQEREQILTVWGNTAAEYPSEQTIHGLFETQTAQTPEQPALFFEG
                     EQLTYRELNERANRLARTLRSQGVTKDRLVGLMTERSVDMIVGIFGILKAGGAYVPIDP
                     TYPEERIRYMLDDSGAKLLLTQNHLVDKVAFDGSVLVL"
     gene            complement(<4240..>7500)
                     /locus_tag="JYU28_RS27065"
                     /old_locus_tag="JYU28_02970"
     CDS_motif       complement(4354..4395)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0013"
                     /evalue="9.10E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="1047"
                     /protein_start="1033"
                     /score="18.8"
                     /tool="antismash"
                     /translation="FGILKAGGAYVPID"
     CDS_motif       complement(4921..5037)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0012"
                     /evalue="3.60E-18"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="858"
                     /protein_start="819"
                     /score="58.0"
                     /tool="antismash"
                     /translation="QKQALTSQDYETYPLYEIQALTEQKQDLINHIIVFENYP"
     aSDomain        complement(5080..5976)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS27065_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.90E-111"
                     /label="JYU28_RS27065_Condensation_DCL.1"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="805"
                     /protein_start="506"
                     /score="362.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILMDG"
                     /tool="antismash"
                     /translation="ENVYALTPMQKGMLFHSLMDAASGAYFEQTTFDLDGRFQADIFQD
                     SLNHLAQRHEIFRANFISGWQDEPVQVIFRRKEVGFRCEDLRHLNEQERDAYVQEFIRQ
                     DKAAGFDLSRDALMRVAILRTGDEDYYFVWSSHHILMDGWCVALVTDEVFEAYFAAVEH
                     RQPKLAPVTPYSQYIEWLEAQDEEAAASYWKDYLLGYEQQTSIPTGKVGAKADGAQFEH
                     ILCDLGKDLTGRMEMVAKQHHVTPNTLLQTAWGLLLQKYNGSDDAVFGGVVSGRPAEIS
                     GIENMVGLFINTIPIRIR"
     CDS_motif       complement(5086..5181)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0011"
                     /evalue="1.60E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="803"
                     /protein_start="771"
                     /score="59.1"
                     /tool="antismash"
                     /translation="DAVFGGVVSGRPAEISGIENMVGLFINTIPIR"
     CDS_motif       complement(5422..5460)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0010"
                     /evalue="3.30E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="691"
                     /protein_start="678"
                     /score="10.2"
                     /tool="antismash"
                     /translation="PYSQYIEWLEAQD"
     CDS_motif       complement(5506..5568)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0009"
                     /evalue="5.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="663"
                     /protein_start="642"
                     /score="34.9"
                     /tool="antismash"
                     /translation="HHILMDGWCVALVTDEVFEAY"
     CDS_motif       complement(5791..5907)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0008"
                     /evalue="2.30E-15"
                     /label="C2_DCL_024-062"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="568"
                     /protein_start="529"
                     /score="48.8"
                     /tool="antismash"
                     /translation="GAYFEQTTFDLDGRFQADIFQDSLNHLAQRHEIFRANFI"
     CDS_motif       complement(5926..5967)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0007"
                     /evalue="3.90E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="523"
                     /protein_start="509"
                     /score="15.5"
                     /tool="antismash"
                     /translation="YALTPMQKGMLFHS"
     aSDomain        complement(6007..6462)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS27065_TIGR01720.1"
                     /evalue="4.30E-54"
                     /label="JYU28_RS27065_TIGR01720.1"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="496"
                     /protein_start="344"
                     /score="174.5"
                     /tool="antismash"
                     /translation="LSQRIKQVKEGLRRIPQKGIGYGMLRYLSAAREQERFVTEPEISF
                     NYLGQFDQDMQSQSIGVSPYSSGAELSAHAARSHTLDMNGLISEGELQLTISYSKQEYR
                     HETMEQLAEEFKISLQEIIAHCVGRKQQELTPSDVMLKHVTMAELDQL"
     aSModule        complement(6007..7383)
                     /domains="nrpspksdomains_JYU28_RS27065_Epimerization.1"
                     /domains="nrpspksdomains_JYU28_RS27065_TIGR01720.1"
                     /incomplete
                     /locus_tags="JYU28_RS27065"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(6313..6342)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0006"
                     /evalue="3.40E-03"
                     /label="NRPS-E7"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="394"
                     /protein_start="384"
                     /score="10.0"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(6379..6441)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0005"
                     /evalue="5.60E-11"
                     /label="NRPS-E6"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="372"
                     /protein_start="351"
                     /score="34.5"
                     /tool="antismash"
                     /translation="VKEGLRRIPQKGIGYGMLRYL"
     aSDomain        complement(6475..7383)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS27065_Epimerization.1"
                     /evalue="8.60E-159"
                     /label="JYU28_RS27065_Epimerization.1"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="340"
                     /protein_start="37"
                     /score="521.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDT"
                     /tool="antismash"
                     /translation="VGAVKLTPVQHWFFEQNLTDAHHHNQSIMLHSKDGFDEPALRTAM
                     DQIVSHHDALRIVFRPTEHGYEAWNRAVGEGELYTLERIDLSHETEVSAAIEAKVNEIQ
                     AGIQLSEGPLVKLGLFHCPDGDHLLMAIHHLVVDTVSWRILFEDITTAYEQALNGQTAR
                     LPHKTDSFRTWADGQSQYANSPAMAAELEFWKEMEHGVYQPLPKDESPSHSLNRDSENM
                     TVTWTAQETEQLLKQAHRAYNTEMNDLLLTALGLTIHRWAGLERVLVSLEGHGRESILP
                     ELDITRTVGWFTTQFPVVLDMS"
     CDS_motif       complement(6484..6534)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0004"
                     /evalue="3.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="337"
                     /protein_start="320"
                     /score="25.6"
                     /tool="antismash"
                     /translation="DITRTVGWFTTQFPVVL"
     CDS_motif       complement(6937..7002)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0003"
                     /evalue="4.70E-11"
                     /label="NRPS-E2"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="186"
                     /protein_start="164"
                     /score="34.9"
                     /tool="antismash"
                     /translation="LLMAIHHLVVDTVSWRILFEDI"
     CDS_motif       complement(7204..7278)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0002"
                     /evalue="5.00E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="97"
                     /protein_start="72"
                     /score="18.9"
                     /tool="antismash"
                     /translation="FDEPALRTAMDQIVSHHDALRIVFR"
     CDS_motif       complement(7339..7368)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS27065_0001"
                     /evalue="2.10E-04"
                     /label="NRPS-E1"
                     /locus_tag="JYU28_RS27065"
                     /protein_end="52"
                     /protein_start="42"
                     /score="14.1"
                     /tool="antismash"
                     /translation="LTPVQHWFFE"
     assembly_gap    7501..7600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     gene            complement(<7601..8052)
                     /locus_tag="JYU28_RS26350"
     CDS             complement(<7601..8052)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /locus_tag="JYU28_RS26350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_348537753.1"
                     /sec_met_domain="AMP-binding (E-value: 8.6e-25, bitscore:
                     75.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGERLYRTGDLARWMPDGNVDFIGRMDYQVKIRGYRIELGEIET
                     AIQRVPGVRQAVVIDRTDERGHKYLCGYITGEAELRIEEVQAELEAGLPAHMVPARLMQ
                     LETIPLTSNGKIDRKALPEPEGSIHTGAAYVAPRTTVEQVLAAVWAG"
     CDS             complement(8042..>8891)
                     /NRPS_PKS="Domain: PCP (187-253). E-value: 1.9e-27. Score:
                     87.2. Matches aSDomain: nrpspksdomains_JYU28_RS02960_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430460.1"
                     /locus_tag="JYU28_RS02960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYU28_02975"
                     /product="AMP-binding protein"
                     /protein_id="WP_245231526.1"
                     /sec_met_domain="AMP-binding (E-value: 1.9e-40, bitscore:
                     126.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.5e-16, bitscore:
                     48.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PFMQHVYEQGLDMSSLELLITSSDSCSVTDYRVLQERFGADIRIM
                     NSYGVTEAAIDSSFYDEELLKLPSSGNVPIGQAWLNARFYIVDSQLNPVPIGVLGELCI
                     GGAGVARGYLNRADLTAEKFVANPFVPGERLYRTGDLARWMPDGNVDFIGPLTSNGKID
                     RKALPEPEGSIHTGAAYVAPRTTVEQVLAAVWAGVLGVEAVGTQDNFFELGGDSIKALQ
                     VSSRLLQAGYRLEMKDLFSHPTVSALALKVQSVTRLADQSEVPEPCGPDGREIRSQSVR
                     AR"
     gene            complement(8042..>8895)
                     /locus_tag="JYU28_RS02960"
                     /old_locus_tag="JYU28_02975"
     aSDomain        complement(8132..8329)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYU28_RS02960_PCP.1"
                     /evalue="1.90E-27"
                     /label="JYU28_RS02960_PCP.1"
                     /locus_tag="JYU28_RS02960"
                     /protein_end="253"
                     /protein_start="187"
                     /score="87.2"
                     /tool="antismash"
                     /translation="EQVLAAVWAGVLGVEAVGTQDNFFELGGDSIKALQVSSRLLQAGY
                     RLEMKDLFSHPTVSALALKVQ"
     CDS_motif       complement(8237..8266)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02960_0002"
                     /evalue="6.10E-04"
                     /label="PCP_mC"
                     /locus_tag="JYU28_RS02960"
                     /protein_end="218"
                     /protein_start="208"
                     /score="12.9"
                     /tool="antismash"
                     /translation="NFFELGGDSI"
     CDS_motif       complement(8519..8608)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYU28_RS02960_0001"
                     /evalue="1.30E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="JYU28_RS02960"
                     /protein_end="124"
                     /protein_start="94"
                     /score="59.0"
                     /tool="antismash"
                     /translation="PIGVLGELCIGGAGVARGYLNRADLTAEKF"
ORIGIN
        1 tacatactct aaaaatacac tcaacatgga tggtacaaag tgcattgtgg taactttatt
       61 tttttcgatt gccttcacga tttcttccgg atttttttct cctcccggta tcagcaggca
      121 gactttggcc ccagtaaagc tccaccacaa taattcccat accgatacgt caaaggtata
      181 gggagttttc tgcaggatca catcaccttc agctatcggg tattgcttct gcatccagtt
      241 gatccggttg attacggagt agttctctat cattacccct ttgggtttgc cggtagaacc
      301 ggatgtatat attacataag ccaagttttc aggtttacta attaactttg gatttgatac
      361 atccccagta aatagttcct gattttctat gctaataaat tcagcatttc tgtaactacc
      421 tttagcattt ataaatttat cctgtatcag aataatttgg ctgccactgt catcaagcat
      481 aaactggatc ctgtcttcag ggtattcagg agctatgggt aaataagccc ccccagcttt
      541 taggacagcc atgatcccta tgatcatttc cggggaacgt tctaccataa ttcccactac
      601 actatcaggt tttattccct tttctcttaa ttttctggcc agttggtttg ccttttcgtt
      661 tatcagcacc agcacgctac cgtcgaaagc cactttatca accagatggt tttgcgttaa
      721 caacagcttc gcacccgagt cgtccagcat gtagcggata cgctcctctg gataggtcgg
      781 atcgatgggc acatatgcgc ccccggcttt taaaatcccg aagatgccca cgatcatgtc
      841 caccgaacgc tcggtcatca gacccaccag tcgatccttc gtcacgcctt gactgcgtaa
      901 ggttcgagcc agacgattcg cacgttcatt cagctcgcga taggtcaact gctctccttc
      961 gaagaacaac gctggctgtt ccggtgtctg cttggtttgc gcctcgaaca agccatgaat
     1021 cgtttgcccg cttggatact cagcagctgt atttccccat acgctgagaa tttgctcacg
     1081 ctcctgctca cttaccatat ccagctcatg cactcgaatg tccggcttcg ccaccacctg
     1141 ctccaacagc tgtatcagat ggccctgagt ccgttcaacg gcttcccgat catataactt
     1201 cgcattgtat tcaaatcgga ttttcatcgc tttctgcggc atgacaacca gattgaaatc
     1261 atagttggtt tgttcaatca tgccggcatt gcgaatttca aaatccgacg ctgcaccttc
     1321 gccaagttgc tcgacctgct cctccaccgg atagttttca aacacgataa tgtggttgat
     1381 cagatcttgc ttttgctcgc tcaaggcttg aatttcatac agcggatacg tttcataatc
     1441 ttgagaagtc agcgcctgct gttgattcgt tctgatcatg tcagcaaagg tgtcctcagc
     1501 cccactacgg atacgtattg gaatggtgtt aatgaacaag cccaccatat tttcgatccc
     1561 cgtaatatcc gctggacgac cagacactac accgccaaaa acagcgtcgt cactaccgtt
     1621 gtacttttgg agcagcaagc cccaggctgt ttgcagcaat gtattcgcgg tcacgtggtg
     1681 ctgcttagcc accatttcca tacgtccggt caagtccttg ccgagatcgc acaggatatg
     1741 ctcaaattgt gcaccgtccg ctttcacgcc tacttttccg gtcggaatcg aagtttgctg
     1801 atcatagccg agcaaataat ccttccagta ggaagctgct gcttctacat cctgtgcttc
     1861 aagccactca atatactgac tgtatggagt cacgggggca agcttcggct gtctatgctc
     1921 cacggctgca aaataagcct caaacacctc atccgtgact aacgccacac accagccatc
     1981 catcaagatg tgatgagagc tccacacaaa ataataatcc tcatcacctg tccgtaaaat
     2041 cgccacgcgc atcaacgcat cccgggacaa atcgaacccg gctgccttgt ctttgcgaat
     2101 aaattccttc acatacgcgt cacgctcctg ctcattcaga tgacgcaaat cttcacatct
     2161 aaaaccaaca tccttgcggc ggaaaatgac ctgcacaggc tcatcctgcc aaccgctgat
     2221 gaaattagct ctaaatatct catgccgctg cgccaaatgg ttcaagctat cctggaaaat
     2281 atccgcttgg aagcgtccat gcaaatcaaa ggtcgtttgt tcaaaataag cacctgactc
     2341 tgcatccatc agactatgga acaacatgcc tttttgcatc ggcgtcagcg catagacatt
     2401 ttccagttcc cccatgtcac gagtccgttc taccaattga tccagctcgg ccatggttac
     2461 atgtttaagc atgacatcgc ttggcgtcag ctcttgctgt ttgcggcttg tgcaatgcgt
     2521 aatgatttcc tgcaaactga ttcggaactg ttcggctaac tgctccatcg tttcacgacg
     2581 atattcctgc ttgctgtagc tgatcgtcaa ttgcagcttg ccttccgaaa tcagtccgtt
     2641 catatccagc gtgtgcgttc tagctgcatg ggcgctaagc tctgcaccgc tagcgtaagg
     2701 agatacacca atggattgat tttgcatatc ttggtcaaat tgtcccaaat agttaaagct
     2761 gatttcaggt tcggttacaa aatgctcttg ctcacgtgct gcggacaggt atctcaacat
     2821 accgtagcca atgccctttt gcggaatgcg acgtagtcct tctttcacct gtttgatgcg
     2881 ctgtgaaagg tcttgcccca cgctcatatc cagcacaacc gggaattggg tcgtaaacca
     2941 gcccacggtg cgcgtaatat ccagttctgg cagaatggac tcccgtccat gaccttccag
     3001 gctcacgagc attcgttcca ggcccgccca gcgatgaatc gtcagtccca atgcggtaag
     3061 cagcaaatca ttcatctccg tgttgtaagc acgatgcgct tgttttaaca gctgctccgt
     3121 ttcctgagct gtccacgtaa ctgtaatgtt ctcgctgtcc ctatttaagg aatgagaagg
     3181 ggactcgtct tttggaagcg gctgatatac gccttgttcc atttcctgcc agaatgcgag
     3241 ttctgctgcc atggccggac tgttcgcata ttgagactgc ccatccgccc aggtgcggaa
     3301 cgaatccgtt ttgtgcggaa gacgagccgc ttgtccgttc aatgcctgct cataggcggt
     3361 cgtaatatcc tcgaacaaaa tgcgccaaga tacggtatcc accactaagt gatgaatagc
     3421 tatcagcaaa tgatctccat cggggcaatg gaacagcccc agcttcacca gcggtccttc
     3481 gcttaaatga atacccgcct gaatttcatt cactttggcc tcgatcgccg cagatacctc
     3541 agactcattt ctgaagtcgg cccgatccag cgtatacagc tcgccctcac cgaccgcgcg
     3601 gttccaagct tcatatccgc gctccgtagg tcggaaaatg atacgcagtg cgtcgtgatg
     3661 gctaacgatt tgatccatcg ccgtgcgcaa tgccggttca tcaaaaccgt ccttgctatg
     3721 cagcataatg gactggttat gatgatgagc atccgtcagg ttctgctcaa agaaccattg
     3781 ttgcaccggt gtcagcttca cagcgcccac cacttcgctc tgatccgcca atcgggtcac
     3841 cgattggact ttcagtgcca aggcagacac ggttggatga ctgaacaggt ctttcatctc
     3901 cagtcgataa cctgcttgca gcaatcggga ggatacttgc aaggctttga tcgaatcgcc
     3961 acccagttca aagaagttat cctgcgtccc taccgcttcc acgccgagta ctccggccca
     4021 taccgcagcc agtacttgct ccaccgtggt acgcggtgcc acataggctg cacccgtatg
     4081 gatgcttcct tccggttccg gcaaggcctt acggtcgatt ttaccgttgc tggtcagcgn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cagcaccagc acgctaccgt
     4261 cgaaagccac tttatcaacc agatggtttt gcgttaacaa cagcttcgca cccgagtcgt
     4321 ccagcatgta gcggatacgc tcctctggat aggtcggatc gatggggaca tatgcgcccc
     4381 cggcttttaa aatcccgaag atgcccacga tcatgtccac cgaacgctcg gtcatcagac
     4441 ccaccagtcg atccttcgtc acgccttgac tacgcaaggt gcgcgccaga cgattcgcac
     4501 gttcattcag ctcgcgatag gtcagctgct ctccttcaaa gaacaacgct ggctgttccg
     4561 gtgtctgcgc cgtttgtgtc tcgaacaagc catgaatcgt ttgctcgctt ggatactcag
     4621 cagctgtatt gccccatacg gtgagaattt gctcacgctc ctgctcactt accatatcca
     4681 gctcctgcac ccgaatgtcc ggcttcgcta ccacctgctc caacagctgt atcagatggc
     4741 cctgagtccg ttcaacggct tcccgatcat ataacttcgc attgtattca aatcggattt
     4801 tcatcgcttc ctgcggcata acgaccagat tgaaatcata gttggtttgt tcaatcatgc
     4861 cggcattgcg aatttcaaaa tccgacgctg caccttcccc aagttgctcg acctgctcct
     4921 ccaccggata gttttcaaac acgataatgt ggttgatcag gtcttgcttt tgctcggtca
     4981 aggcttgaat ttcatacagc ggatacgttt cataatcttg agaagtcagc gcctgtttct
     5041 gattcgttct gatcaggtcg gcaaaggtat cgtcagcccc actgcggata cgtattggaa
     5101 tggtgttaat gaacaagccc accatgtttt cgatccccga aatctccgct ggacgacccg
     5161 acactacacc gccaaaaaca gcgtcgtcac taccgttgta cttttggagt agcaagcccc
     5221 aggcggtttg cagcaatgta ttcggggtca cgtggtgctg cttggccacc atttccatac
     5281 gtccggtcaa gtccttgccc agatcgcaca ggatatgctc aaattgtgca ccgtccgctt
     5341 tcgcgccgac ttttccggtc ggaatcgaag tttgctgctc atagccgagc aaataatcct
     5401 tccagtagga agctgctgct tcttcatcct gtgcttcaag ccactcaata tactgactgt
     5461 atggagtcac gggggcaagc ttcggctgtc tatgctccac ggctgcaaaa taagcctcaa
     5521 acacctcatc cgtgactaac gccacacacc agccatccat caagatgtga tgagagctcc
     5581 acacaaaata ataatcctca tcacctgttc gtaaaatcgc cacgcgcatc aacgcatccc
     5641 gggacaaatc gaatccggct gccttgtcct ggcgaataaa ttcctgcaca tacgcgtcac
     5701 gctcctgctc attcagatga cgcaaatctt cacatctgaa accaacctcc ttgcggcgga
     5761 aaatcacctg cacaggctca tcctgccaac cgctgatgaa attagctcta aagatctcat
     5821 gccgctgcgc caaatgattc aagctatctt ggaaaatatc cgcttggaag cgtccgtcca
     5881 aatcaaaggt cgtttgttca aaataagctc ctgacgctgc atccatcaga ctatggaaca
     5941 gcatgccttt ttgcatcggc gtcaacgcat atacattttc cagttccccg atgtcacgag
     6001 tccgctctac caactgatcc agctcggcca tggttacatg tttaagcatg acatcgcttg
     6061 gcgtcagttc ttgctgtttg cggcctacac aatgcgcaat gatttcctgc aaactgattt
     6121 tgaactcttc agctaactgc tccatcgttt catgacgata ttcctgcttg ctgtagctga
     6181 ttgtcaattg cagttcgcct tccgaaatca gtccgttcat atccagcgtg tgcgatctgg
     6241 ctgcatgggc gctaagctct gcaccgctag agtaaggaga tacgccaatg gactgacttt
     6301 gcatatcttg atcaaattgt cccagatagt taaagctgat ttcaggttcg gtcacaaaac
     6361 gctcttgctc acgggctgcg gacaggtatc tcaacatccc gtagccaatg cccttttgcg
     6421 gaatgcggcg gagtccttct ttcacctgtt tgatgcgctg tgaaaggtct tgccccacgc
     6481 tcatatccag caccaccggg aattgggtcg taaaccagcc caccgtccgc gtaatatcca
     6541 gttctggcaa aatggactcc cgtccatggc cttccaggct cacgagcact cgttccaagc
     6601 ccgcccagcg atgaatagtc agtcctaagg cggtaagcag caaatcattc atctccgtgt
     6661 tgtaggcacg atgcgcctgt tttaacaact gctccgtttc ctgagctgtc cacgtaactg
     6721 tcatgttctc gctgtcccta ttcaaggaat gagaagggga ctcgtctttt ggaagcggct
     6781 gatatacgcc atgttccatt tccttccaga attcaagttc tgctgccata gccggactat
     6841 tcgcatattg agactgccca tccgcccagg tgcggaacga atccgttttg tgcggaaggc
     6901 gagccgtttg tccgttcaat gcctgctcgt aggcggttgt aatatcctcg aacaaaatgc
     6961 gccaagatac ggtatccacc accaagtgat gaatcgccat cagcaaatga tcaccatctg
     7021 ggcaatggaa cagccccagt ttcaccagcg gtccttcgct taactgaata cccgcctgaa
     7081 tttcattcac tttagcctcg atcgccgctg acacctcagt ctcatggctg aggtctatac
     7141 gttccagcgt atacagctcg ccctccccaa ccgcacggtt ccaggcttca tatccgtgct
     7201 ccgtaggacg gaaaacgatc cgcagtgcgt cgtgatggct aacgatttga tccatcgccg
     7261 tgcgcagggc cggttcatca aaaccgtcct tgctatgcag catgatggac tggttatgat
     7321 gatgagcatc cgtcaggttc tgctcaaaga accagtgttg caccggtgtc agtttcacag
     7381 cgcccaccac ttcgctctga tccgccaatc gggtcaccga ttggactttc agtgccaagg
     7441 cagacacggt tggatgactg aacaggtctt tcatctccag tcgataacct gcttgcagca
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggcccata ccgcagccag
     7621 tacttgctcc accgtggtac gcggtgccac ataggctgca cccgtatgga tgcttccttc
     7681 cggttccggc aaggccttac ggtcgatttt accgttgctg gtcagcggga tcgtctccag
     7741 ttgcatcaaa cgtgcaggca ccatgtgcgc tggcagtccg gcttccagtt cggcctgtac
     7801 ctcctcgatc cgcagctcag cttctcctgt gatgtatccg cacaagtact tgtgtccacg
     7861 ctcatccgtc cggtcgatga ctaccgcttg acgtacaccc ggcacccgct ggatcgccgt
     7921 ttcgatctcg cccagctcga tccgatagcc ccggattttc acttgatagt ccatccggcc
     7981 gataaagtcc acattgccgt ccggcatcca tcgcgccaag tcacctgtgc ggtataatcg
     8041 ttcacctggc acgaacggat tggctacgaa tttctcggcc gtcaggtccg cacggttcag
     8101 gtacttcgct ctgatccgcc aatcgggtca ccgattggac tttcagtgcc aaggcagaca
     8161 cggttggatg actgaacagg tctttcatct ccagtcgata acctgcttgc agcaatcggg
     8221 aggatacttg taaggctttg atcgaatcgc cacccagctc aaagaagtta tcctgcgtgc
     8281 ctaccgcttc cacgccgagc accccggccc ataccgcagc cagtacttgc tccaccgtgg
     8341 tacgcggtgc cacataggct gcacccgtat ggatgcttcc ttccggttcc ggcaaggcct
     8401 tacggtcgat tttaccgttg ctggtcagcg ggccgataaa gtccacattg ccgtccggca
     8461 tccatcgcgc caagtcacct gtgcggtata atcgttcacc tggcacgaac ggattggcta
     8521 cgaatttctc ggccgtcagg tccgcacggt tcaggtatcc ccgtgctacc ccagctccgc
     8581 caatgcacaa ctcgcccagt acgccgatcg gcaccggatt cagctggctg tccacgatgt
     8641 agaagcgtgc gttcagccat gcctgtccaa tcggcacatt accgctgctt ggcagcttca
     8701 acagttcttc gtcgtagaag ctggagtcga tcgccgcttc cgtgacaccg tagctgttca
     8761 taatgcggat atctgcgcca aatcgttctt gcaacacccg ataatccgtc acgctgcagc
     8821 tatccgagct ggtaatcagc aattccaaac tgctcatgtc cagtccctgc tcgtatacat
     8881 gctgcatgaa cggta
//
