LOCUS       NZ_CP071839            10990 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6982756
            Orig. end                         :: 6993746
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="26"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5990)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    868..881
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     misc_feature    complement(1078..1091)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.55"
                     /tool="antismash"
     misc_feature    complement(1149..1162)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.96"
                     /tool="antismash"
     gene            complement(1206..2234)
                     /locus_tag="S1361_RS31325"
                     /old_locus_tag="S1361_31290"
     CDS             complement(1206..2234)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="S1361_RS31325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31290"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_208035246.1"
                     /transl_table=11
                     /translation="MSVLDEWTGRRAAGRADLGTPGPDQTDDVVLRAMTDARDVDDFDM
                     RHLRYFLTVVRAGTVSAAAQQLRISQPSLSQQIRRLEQRVGAPLFIRSSRGVELTSGGR
                     AFLREIQGIPGQLRSAIAAAAPAPRIWSVGVCGGVPADVLIEVQNALSGSRPDSGRTGE
                     ATLRMRPADAADQGELLRHGEIAFGIVRLPIPMPHVVRAVVRDEPLGVVMAREHPLAAR
                     TDFTWSDLDRQRLLWYEDGCAPGFAQTVPAQLARLGWSAVLHPVNQDQDALFVHALQTT
                     GDLVALRPRRAVEREPHLVWRPLPTTRPPRERLAVITVSGGRTARVLREVSARHGWTFL
                     AE"
     misc_feature    2343..2352
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.37"
                     /tool="antismash"
     gene            complement(2349..2978)
                     /locus_tag="S1361_RS31330"
                     /old_locus_tag="S1361_31295"
     CDS             complement(2349..2978)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="S1361_RS31330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31295"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_208035247.1"
                     /transl_table=11
                     /translation="MATQERARRTREKVLTAAAEVFAAQGYDRATLTAVAERIGMTKGA
                     LYGHFPSKRSLAGALIDESRQAWTSLRTEHDAPGADAGGVLERVVMGFAGRLQTDVRLR
                     ATIRLAADCPALARALYDVLAEVRVALTELVRRAQRDSGFPAYPPRLVAHLLMIVMYGL
                     LQAPAGCAGDNGTPGGEPLWRLLFTALSGAEVPGAVREEDEVVSGQ"
     misc_feature    complement(2994..3009)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     gene            complement(3062..3763)
                     /locus_tag="S1361_RS31335"
                     /old_locus_tag="S1361_31300"
     CDS             complement(3062..3763)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="S1361_RS31335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31300"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_208035248.1"
                     /transl_table=11
                     /translation="MTTVATRTHPALAFFDVDETVIAEKSMIVFWEHWVNLHPAGIAAQ
                     APGPPAGAVAPVDRAELNRRHYRRYAGVPLAALEAAGQVWYDGYRRGGAAFVAPVVEAV
                     AAHRAAGREVVLVSGSMRPLLTPLARDVGASTILCTELVVGDDGVLTGEVRRPMIGDAK
                     AEAAVRLMRERGADPRECFAYGDHESDLALLRAVGRPVVVGDSPQLNEAAERFGWPVAP
                     ARLGPLRAESA"
     gene            complement(3760..5007)
                     /locus_tag="S1361_RS31340"
                     /old_locus_tag="S1361_31305"
     CDS             complement(3760..5007)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /locus_tag="S1361_RS31340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31305"
                     /product="oxidoreductase"
                     /protein_id="WP_208035249.1"
                     /transl_table=11
                     /translation="MLSERPGVRRDDPPLGEDIDPTGGETDPTGERIPGGGSEQRGPGD
                     MTDRHRTDGRDGLGRLLEVAGQDARGADERRRLDPRVAAAVVDAGFARHFVPAKWGGTA
                     GSFARLLADASAVAEACAATGWCAALYAAHGRLAAYLPERGQREVWAHGPDVRIAASVV
                     PPQGNATPVPGGWRLDGEWRTASGVDHAEWVLLASWTPGLDGTREHRLFAVPRAELSVR
                     DTWDSVGLRGSGSNSVTGEGITVPAHRSFTLGALLRPLPDAARCHRVPYPMVAAPIFAA
                     AVLGAARAALRAWTAECVRPEARRPGQDLLLASASARVHAAGLLLEGTAVRADRGEITP
                     LTVAENRRDAATAAAWCREAANDLFHASGMRGQSPHSRVQRAWRDVTTAASHGALGLDA
                     AADAYADAALAGGDTR"
     gene            complement(5001..5990)
                     /locus_tag="S1361_RS31345"
                     /old_locus_tag="S1361_31310"
     CDS             complement(5001..5990)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="S1361_RS31345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31310"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_243769570.1"
                     /sec_met_domain="AfsA (E-value: 1.6e-18, bitscore: 65.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGLEMRTDRPGPELYWGRLNFDHRVPRESVHKAADAEVFLTDAE
                     RLGDERFAVAAAWRRDHYLGHHDGCAADPVLLAETARQTAIHLSHRFLDVPHGMPFVLN
                     AVTVDLTEALPPLGAHGLAVGLDVVCRPPAEGARRLRLELEADVLVQRRRLGRVGVHWE
                     PMEPRRYALLRRRVERSPEGERPRPAPPRVPLPPARVGQLAERDVLIEADPLRANGWWL
                     RLDQEHPVLFDHESDHVPGMALVEAFRQASQVAAADGEPGAGAVTLIVVRFTAFGELDA
                     PVAIIAEPAEGGGRTFTVRARQGDRELARATVRRGMAEPALTRLDAAC"
     misc_feature    6076..6096
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.22"
                     /tool="antismash"
     gene            6178..6825
                     /locus_tag="S1361_RS31350"
                     /old_locus_tag="S1361_31315"
     CDS             6178..6825
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039544.1"
                     /locus_tag="S1361_RS31350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31315"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_208035251.1"
                     /transl_table=11
                     /translation="MSEPQATAFAQPRTTVRRGSDPKQERSARTRLRVLIGAAELFTER
                     GFRHTSVKDVADRVEMTKGAVYFHYPTKESLAVAIVEEHYGRWPKILDEVTKEGRGPLD
                     TAARMLECAAIAFQDDVIVQAGARLQLERPQIDAELPTPYVGWVSLLESLLTSAQEAGE
                     LRPGVEPGRAARSLVSAFFGSQHVSDVLSGRADVVERWRDLSELLFRSIRGD"
     gene            complement(7257..8519)
                     /locus_tag="S1361_RS31355"
                     /old_locus_tag="S1361_31320"
     CDS             complement(7257..8519)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387370.1"
                     /locus_tag="S1361_RS31355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31320"
                     /product="MFS transporter"
                     /protein_id="WP_208035252.1"
                     /transl_table=11
                     /translation="MSEPRTATVQAIDGRRRTDRADTGGRSVAPLWWVWLAAWPVTAVF
                     VLSNAATPLYVLWQRDIGFSKGTLTVVFAFYIIGLIGSLLVSGVVSDRLGRKPVLLPAL
                     VLALAACAIFATAASVAALIVARLFTGIAVGAVVSAGMAAVTDVAGPERKRIAALLASC
                     AMVFGAGLGPLLAGVLSETVPGPTVTVFVVEAALLVTAVLCVLRMPVRRPEVRAKGAWI
                     RVPGVPQGNGRQLALGIAVFAPGITATSFVLSLGPSLLSGLLDTTNRIVAGAMAFVMFL
                     AATGVQFAVQRLRRRTILTAGAVSTTLSMIMLVVAVHTSAVAVLILSALLAGAGQGMGQ
                     LGGLSLLNSSIPPQRLAEANAALNVGGYIPAGALPVSAGYLSDAVGLTNGATIFGAVLM
                     GVAVIGGLVVLATRRQVTDPA"
     gene            8631..9008
                     /locus_tag="S1361_RS31360"
                     /old_locus_tag="S1361_31325"
     CDS             8631..9008
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387395.1"
                     /locus_tag="S1361_RS31360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31325"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_208035253.1"
                     /transl_table=11
                     /translation="MPAPTAAAPAVKVFPEPPDLPEPLPEPGVDELRLETVLGALSDPL
                     RLTIVRKLLLESENFDHSCGWFGFDRPKSSLTHHFKALREAGITRQRQYGLERRSHVRV
                     DDLNARFPGLLDLVAAWTPQA"
     gene            complement(9124..9327)
                     /locus_tag="S1361_RS31365"
                     /old_locus_tag="S1361_31335"
     CDS             complement(9124..9327)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482217.1"
                     /locus_tag="S1361_RS31365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31335"
                     /product="hypothetical protein"
                     /protein_id="WP_208035254.1"
                     /transl_table=11
                     /translation="MKRLLEFLGFLALMQGTGGLAYEITGRLHWGITLRWGVLDGYEIY
                     ASIALILLALALFAAAESRRPG"
     gene            9528..10787
                     /locus_tag="S1361_RS31370"
                     /old_locus_tag="S1361_31340"
     CDS             9528..10787
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983430.1"
                     /locus_tag="S1361_RS31370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_31340"
                     /product="histidine kinase"
                     /protein_id="WP_208035255.1"
                     /transl_table=11
                     /translation="MSTDEWARAPKVFTGRRWLFPSALVHELDPDAAASGRRPRRTARD
                     WVVDFFCFLLAVLIGLLSADSMRQEDLPDGYAALDQALGALACGAVWLRRRWPVGLAVA
                     LVPVAFLSNTAGGAGVVALFTLAVHRPLRYVAWAGGASVALVPVFFWLRPDPDVPYVVA
                     VASGVLLTAAMVGWGMFVRSKRQLVLSLRDRARHAETEARLRAEQAQRLAREAIAREMH
                     DVLAHRLTLLSVHAGALEFRPDAPREEVARAAGVIRESAHEALQDLREIIGVLRAGEAD
                     EPPGGRPQPTLAALDALVAECREAGMNVALDHRVADPAAVPASVGRTAYRIAQEALTNA
                     RKHAPGTDVTVRLAGTPGDGLTVTVTNPAPSSEVPPVPGSGQGLIGLTERASLAGGHLE
                     HGTRPDGGFEVRGWLPWPRT"
ORIGIN
        1 tcgaggatct cgccggcgaa catggcgcgc tggctggtgt cgtcgcgggc gacggcggcg
       61 gtccactcgg gctccaccgc ggcgcggcgg gcggcgagtt cggcgtcccg gccggtccag
      121 gtgcgggtga agtcgttgct cacggagcgg gcctggtagg cctcgtccca cgggatgccg
      181 cgcacgacgt cgaacgcccg ggtgttgacg gtgtccgcgg tccgcgcgga gaccagacgg
      241 gacttgaatc cggcggtggc cagcgactcc tcggtggcgg cgaaccgggt gcccatcatc
      301 acgccgtcgg cgccgagcgc gagggcggcg gcgagcccgc ggccgtccgc gaacccgccg
      361 gccgcgacca ccggcacctg cccctgggcg acgtcgagta cggcgggaac gaggggcagc
      421 gtggccagcg acgcgtggtg cccgccggcc tccgcgccct gggccacgag caccgacgcc
      481 ccggcctgta cggcgacggc ggcctgctcg gggtcgtgca cctgcaccac gacatgcgcc
      541 ccgctgtcgg ccgcggacgc cacgtacttg tggacctgcg ccgcgtcacc gaaggagagg
      601 gcgatgaccg gcggggcgta accgagcacc ttgtcccaca ggccgggccg ctcgtcgaag
      661 gtgaacatga cgcagccgac gccccagaca ccgccgttct ccgcggcctg ggccacgtgc
      721 tcgtcgatga acgccgcgtc gccgtagctg acgccgacga ggccgaggcc gcccgcccgg
      781 gtcacggcgc cggcgagccg tccgccggac accgaaccca tcggggcgca gacgaccgga
      841 tggtcgatgc ccagcaactc ggtcagcttg gtcgagatca cgatgctccc ttggtccggg
      901 ttcgatggca tggcacggcc cgacgggccg aggaggaaaa gaagcgccgg cgaattccac
      961 actgaattcc gcgcgagaaa cagacggggg gttccgggaa gggaaaccgc ttcggaagag
     1021 gcccgtgctg ttttacgttc aagaggctag cctccgtcgg ccacggccgt ccaagacgca
     1081 atccgcatgc cagccataag cgggcgccta taccggtggc cgcatcacgt gccgtatcgc
     1141 tgcggaaaca aaaaagcgtg tgatcccggc cggggccggg atcacacgcg gacaggggca
     1201 gggggctact cggcgaggaa cgtccagccg tgccgcgccg agacctcgcg caggacgcgg
     1261 gcggtgcggc cgccgctgac ggtgatgacg gcgaggcgct cgcgcggcgg cctggtcgtg
     1321 ggcaggggtc gccagaccag gtgcggttcc cgctccaccg cgcgccgcgg acgcagggcc
     1381 acgagatccc cggtggtctg cagggcgtgc acgaacagcg cgtcctggtc ctggttgacc
     1441 gggtgcagca ccgcgctcca gccgagccgg gccagctgcg ccggcacggt ctgggcgaag
     1501 cccggcgcgc agccgtcctc gtaccacagc aggcgctgcc ggtcgaggtc gctccaggtg
     1561 aagtcggtgc gtgcggcgag cggatgctcc cgcgccatga cgacgccgag cggttcgtcg
     1621 cggaccacgg cgcgcaccac gtgcggcatc gggatcggca ggcgcacgat gccgaaggcg
     1681 atctcgccgt ggcgcagcag ttccccctgg tccgccgcgt ccgccggacg catgcgcagg
     1741 gtcgcctccc ccgtgcggcc gctgtccggg cggctcccgg acagcgcgtt ctgcacctcg
     1801 atgaggacgt ccgccggcac gccgccgcac actccgacgg accagatccg cggggcggga
     1861 gcggccgcgg cgatggccga gcgcagctgg cccggaatcc cctggatttc cctgagaaag
     1921 gcccggccgc cgcttgtgag ttcaacgccg cgcgaactgc gaatgaacag gggggcgccg
     1981 acgcgctgtt ccaggcgtcg aatttgctga ctgaggctcg gttgtgaaat ccggagttgc
     2041 tgggccgcgg ccgatacggt tccggcgcgt accaccgtca ggaaataccg aaggtggcgc
     2101 atgtcgaagt cgtcgacgtc acgtgcgtcc gtcatcgcgc gcagcacgac gtcgtccgtc
     2161 tgatccggtc ctggtgtgcc gaggtccgcg cgccccgctg cgcgccgacc cgtccactcg
     2221 tccagcacgc tcatccgctt cctcccaaac gttgccaccc ctgccccgtg cagggatgca
     2281 cacccggccc ggcgcccgcc ggggtcccgg tgcagcatga cagaacaatt cgttctcgaa
     2341 ggtatttttt actggccgga aacgacctcg tcctcttcgc gtaccgcgcc cggcacctcc
     2401 gcgcccgaca gggcggtgaa cagcagccgc cacaacggct ctccgccggg tgtgccgttg
     2461 tcgccggcac accccgcggg cgcctggagc agcccgtaca tcacgatcat cagcagatgg
     2521 gccacgagcc gcggcgggta cgcggggaag ccgctgtcgc gttgcgcccg gcggaccagt
     2581 tcggtcagcg cgacgcgcac ctccgcgagg acgtcgtaga gcgcccgggc cagggccgga
     2641 cagtcggccg cgagccggat cgtcgcccgc agccgcacat ccgtctgcag ccgcccggcg
     2701 aaccccatga cgacccgttc gaggacgccc ccggcgtcgg cgcccggagc gtcgtgctcg
     2761 gtgcgcagac tcgtccacgc ctgccgggac tcgtcgatga gagccccggc cagcgaccgc
     2821 ttcgagggga agtgcccgta gagcgcgccc ttcgtcatgc cgatccgctc ggccacagcg
     2881 gtgagggtgg ccctgtcgta accctgcgcg gcgaacacct cggccgcggc cgtcagcacc
     2941 ttctccctgg tccgccgtgc ccgttcctgt gtggccatgt ttgtgccctc catcgtgaaa
     3001 gagagcacat acataccatc ggggcggaat gctctcgccg cgttccggcc tcgcggaccc
     3061 ctcacgcgct ctccgccctc aacggcccca ggcgggcggg cgccaccggc cagccgaagc
     3121 gctccgcggc ctcgttcagc tggggggaat cgcccaccac cacgggccgg cccaccgcgc
     3181 gcagcagggc gaggtcgctc tcgtggtcgc cgtaggcgaa acactcccgg gggtccgcgc
     3241 cgcgctcccg catgagacgg accgcggcct ccgccttggc gtccccgatc atcgggcggc
     3301 gcacctcgcc cgtcagcacc ccgtcgtcac ccaccaccag ttcggtgcac aggatcgtcg
     3361 aggctcccac gtcccttgcc agtggggtca gcagcgggcg catcgagccc gagaccagca
     3421 ccacctcgcg gccggcggca cggtgcgcgg cgacggcctc gacgacgggc gccacgaagg
     3481 ccgctccgcc gcggcggtag ccgtcgtacc agacctggcc ggccgcctcc agggcggcca
     3541 ggggcacgcc cgcgtaacgc cggtagtgcc ggcggttcag ctccgcgcgg tcgacgggcg
     3601 ccaccgcccc ggccggcggc ccgggcgcct gggcggcgat gccggccgga tgcagattca
     3661 cccagtgttc ccagaagacg atcatgctct tctcggcgat cacggtctcg tcgacgtcga
     3721 agaaggcgag cgcggggtgc gtcctcgtgg ccaccgtcgt catcgcgtgt ccccgcccgc
     3781 cagagccgcg tccgcgtagg cgtcggccgc cgcgtcgagg ccgagcgcgc cgtgggacgc
     3841 ggccgtcgtg acgtcccgcc aggcccgctg cacccggctg tgcggcgact gcccgcgcat
     3901 gccagaggcg tggaacaggt cgttcgccgc ctcgcggcac caggcggccg cggtcgccgc
     3961 gtcccggcgg ttctcggcca ccgtgagcgg cgtgatctcg ccccggtcgg cgcgcacggc
     4021 ggtgccctcc agcagcaggc ccgccgcatg gacgcgggcg gacgccgacg ccagcagcag
     4081 atcctggccc ggtctgcgcg cctcgggccg gacgcactcg gccgtccagg cccgcagcgc
     4141 cgcccgggcc gcccccagca ccgccgcggc gaagatcggc gccgcgacca tcggatacgg
     4201 gacgcggtgg cagcgggcgg cgtcgggcag cgggcgcagc aacgcgccca gggtgaacga
     4261 ccgatgggcg ggcacggtga tcccttcccc cgtcacgctg ttgctgccgc tcccgcgcag
     4321 gccgacggag tcccaggtgt cccggaccga caactccgcg cgcggcacgg cgaagagacg
     4381 gtgctcgcgg gtgccgtcga gccccggtgt ccaggacgcc agcagcaccc actcggcgtg
     4441 gtcgacaccg ctggccgtcc gccactcccc gtcgagccgc cagccgccgg gcacgggcgt
     4501 ggcgttgccc tgcggcggca cgaccgacgc ggcgatacgg acgtccgggc cgtgcgccca
     4561 cacctcccgc tgtccccgct cgggcagata ggcggcgagc cggccgtggg ccgcgtacag
     4621 ggcggcgcac caaccggtgg cggcgcaggc ctccgcgacg gccgaggcgt cggcgagcaa
     4681 ccgggcgaaa ctcccggccg tcccacccca cttggccggg acgaagtgac gcgcgaaccc
     4741 cgcgtcgacg acggcggcgg cgacccgcgg gtcgagccgg cgccgctcgt cggcaccgcg
     4801 tgcgtcctgt ccggcgacct cgaggagccg gccgagtccg tcccgcccgt ccgtgcggtg
     4861 gcggtcggtc atgtcgccgg gcccgcgttg ctcgctcccg ccgccgggta tccgctcgcc
     4921 cgtggggtcg gtttccccgc ccgtggggtc gatgtcctcg ccgaggggcg ggtcatcgcg
     4981 ccgtacgccg ggccgttcgc tcagcatgcc gcgtccagcc gggtcagggc gggttccgcc
     5041 ataccgcggc gcaccgtggc ccgggcgagt tcacggtcgc cctggcgggc gcggacggtg
     5101 aaggtgcgtc cgccgccttc ggcgggttcg gcgatgatcg ccaccggcgc gtccagttcg
     5161 ccgaacgccg tgaaccgcac gacgatcagg gtcaccgcgc cggcgcccgg ctcaccgtcg
     5221 gccgcggcga cctggctcgc ctgccggaac gcctccacga gcgccatgcc cgggacgtga
     5281 tccgactcgt ggtcgaagag cacggggtgc tcctggtcga gccgcagcca ccagccgttc
     5341 gcccgcagcg ggtcggcctc gatgagcacg tcccgttcgg cgagctggcc gacacgggcc
     5401 ggcggcagcg gcacgcgcgg cggcgccgga cgcggccgct ccccttccgg gctccgctcc
     5461 acgcgccgcc gcagcagggc gtagcggcgc ggctccatcg gctcccagtg cacccccacc
     5521 cgcccgaggc ggcggcgttg caccaggacg tcggcctcga gttccaggcg caggcgccgc
     5581 gcgccctcgg cgggcgggcg gcagacgacg tcgagtccca cggcgaggcc gtgcgcgccg
     5641 agcggcggca gggcctcggt gaggtcgacg gtgaccgcgt tcagcacgaa cggcatcccg
     5701 tgcgggacgt cgaggaaacg gtgggacaga tggatcgcgg tctgccgggc ggtctccgcg
     5761 agcagcaccg ggtcggccgc gcagccgtcg tgatggccga ggtagtggtc ccggcgccac
     5821 gcggcggcga ccgcgaagcg ttcgtcgccg agccgctccg cgtccgtcag gaacacttcc
     5881 gcgtcggccg ccttgtgaac ggactcacgc ggaacgcggt ggtcgaagtt cagcctcccc
     5941 cagtagagct caggccccgg tcggtcggtg cgcatttcga gcccaggcat gacgtacacg
     6001 cgtagattcc tcccctggtc gccgtgactt ccaggcgccg gtatcgtccc gacgcagtac
     6061 gtggcgactc cgaagaaata taccttcggg aaggtatatt ggggggtcat ttttcgtcac
     6121 cgcacagagc cgcacgccgc atccgcgagc atgcccgagg acagggaagc gacgctcatg
     6181 tcagagccgc aagcgaccgc gttcgcccag ccgagaacca cggtcagaag gggatccgat
     6241 cccaaacagg agcgctcggc ccgcacgcgt ctgcgggtcc tgatcggggc ggcggagctg
     6301 ttcaccgagc gcggcttccg ccacacgtcg gtcaaggacg tggcggaccg cgtcgagatg
     6361 accaagggcg ccgtgtactt ccactacccg accaaggaaa gcctggcggt ggccatcgtc
     6421 gaggagcact acgggcgttg gccgaaaata ctcgacgagg tcaccaagga gggccgggga
     6481 ccgctggaca ccgccgcgcg catgctcgag tgcgcggcca tcgcgttcca ggacgacgtg
     6541 atcgtgcagg ccggcgcccg gttgcagctc gaacgcccgc agatcgacgc cgaactgccc
     6601 acgccctacg tcggctgggt ctccctgctg gagtccctgc tcacttccgc ccaggaggcg
     6661 ggcgagctgc gtcccggggt cgaaccgggc cgggccgccc gctcgttggt ctcggcgttc
     6721 ttcggctccc agcacgtctc cgacgtcctc agcggacgcg cggacgtcgt ggagcggtgg
     6781 cgggatctga gcgagctgct ctttcggtcg atccggggtg actgaccggg gaatccggga
     6841 cgactgacgc ttccggggtt cgggcggggg accggtcctc gggccgggag gccggtggcc
     6901 gggtctgggc ggccgacgtg tgaaccgggc cggtcgacgc gtggaccggc gcgcccggcg
     6961 cgggacctgg gtcggccgtc gctttcggcg ctcggcccgg gacggccggc gcgcgatccg
     7021 ggacggccgg cgtacgggcg aggcgcgttg agcgcacgag ccgggccgcc cgagtggtgg
     7081 acacgggccg actgggcgcg cacgtcggcc cattgagcgc gtcggtcttc gcgtacggca
     7141 cgcgccaggg gagcggtgca ccgccgccgt ccagcggtgc accgctcccc aggagcaggc
     7201 cgtccgtggc agcccggtgg gccgcagtcg gccggccggg atcggacggg ccgtggtcag
     7261 gccgggtccg tcacctggcg ccgggtggcc aggaccacca gcccgccgat cacggcgaca
     7321 cccatcagaa ccgcgccgaa gatcgtggcg ccgttggtca ggcccacggc gtcgctgagg
     7381 tagccggcgg agaccggcag ggcaccggcc gggatgtaac cacccacgtt gagtgcggcg
     7441 ttggcctcgg ccaggcgctg cggcgggatg ctggagttga gcagcgacag tccgccgagc
     7501 tggcccatgc cctggccggc gccggccagc agcgcggaga ggatgagcac cgccaccgcc
     7561 gaggtgtgca cggcgacgac cagcatgatc atgctgagcg tggtgctgac cgcgccggcc
     7621 gtgaggatgg tgcggcggcg cagccgctgg acggcgaact gcacgcccgt ggcggcgagg
     7681 aacatcacga acgccatggc gccggcgacg atccggttgg tggtgtcgag caggccggac
     7741 agcagcgagg gaccgagcga gagcacgaag gacgtggcgg tgatgccggg ggcgaacacc
     7801 gcgatgccca gggcgagctg acggccgttg ccctgcggca cgccgggcac ccggatccag
     7861 gcgcccttgg cgcgcacctc gggacggcgc accggcatac gcagcacgca cagcaccgcg
     7921 gtgaccagga gcgcggcctc gacgacgaag acggtcacgg tcgggccggg caccgtctcg
     7981 gagagcacgc cggcgagcag cgggccgagc ccggcgccga agaccatcgc gcaggaggcg
     8041 agcagggcgg cgatccgctt gcgctcgggt ccggcgacgt ccgtcacggc ggccatgccg
     8101 gccgagacga cggcaccgac cgcgatcccg gtgaacagcc gggccacgat cagcgcggcc
     8161 acgctggcgg cggtcgcgaa gatcgcgcac gcggcgaggg cgagcaccag ggcgggcagc
     8221 aggacgggct tgcggccgag ccggtcggag accacgccgg agaccagcag cgagccgatg
     8281 aggccgatga tgtagaaggc gaacaccacg gtcagggtgc ccttggagaa gccgatgtcg
     8341 cgctgccaca gcacgtacaa cggcgtggcc gcgttcgaga gcacgaagac ggcggtcacc
     8401 ggccatgcgg ccagccacac ccaccacagg ggcgcgacgg atcgcccgcc ggtgtccgcg
     8461 cggtctgtac gtcgcctccc gtcgatggcc tggacggttg cggtccgggg ctcggacatg
     8521 actgttcctc ccgatcttcc ctgccggcat ccagcagtac gagtcaagtc gaacttagca
     8581 tgcagtacga tcgaagtcgt acataggaat gccgttatga ggagttgcct atgcccgcgc
     8641 ccacggccgc cgcgcccgcc gtcaaggtgt tccccgagcc gcccgacctg ccggaacccc
     8701 tgccggagcc gggggtggac gagctgcgtc tggagaccgt gctcggcgcg ctcagcgatc
     8761 ccctgcggct gacgatcgtc cgcaaactcc tcctggagtc ggagaacttc gaccactcct
     8821 gcggctggtt cggcttcgac cggcccaagt cgtcgctcac ccatcacttc aaggcgctgc
     8881 gcgaggcggg gatcacgcgc cagcgccagt acggcctgga gcgtcggagc cacgtccggg
     8941 tcgacgacct caacgcccgg ttccccggcc tcctcgacct ggtggcggcc tggacaccgc
     9001 aggcgtgacg gcacctggcg gggccgagcc tggcgacgtg gaggactact gcacccccga
     9061 ccactacgcc acctcggacc tgaccgactt cgaccgccga cccaccgtgc cgggggccgc
     9121 ggttcagccg ggcctgcggc tctcggcggc ggcgaacagg gccagcgcca gcaggatcaa
     9181 cgcgatgctc gcgtagatct cgtagccgtc gaggaccccc caccgcaggg tgatgcccca
     9241 gtgcagcctg cccgtgatct cgtacgccag accgccggtg ccctgcatca gggcgagaaa
     9301 cccgagaaac tccagcaaac gcttcatgga acgactctcg ccccgcggcc tgccgccacg
     9361 catcggccgc ggggcgagcc aacggtgccg aaaggccgtg ccccggccgg tggccggtcg
     9421 ccgaaagtcc accgtggtgc gacttcggtc gacgatcccg gtcctccggt agcgatcgtg
     9481 ggcacggggc gacgacacgg cgccgacagt gcgtagattg gccgaccgtg agcacagacg
     9541 agtgggcgcg ggcgccgaag gtattcacgg gccgccggtg gctgttcccc tccgcgctgg
     9601 tacacgaact ggacccggac gccgcggcct ccggccggcg gccccggcgc acggcgcgcg
     9661 actgggtggt cgacttcttc tgcttcctgc tggccgtgct gatcggcctg ctgagcgcgg
     9721 acagcatgcg gcaggaggac ctgccggacg gctacgccgc cctcgaccag gcgctgggcg
     9781 cactcgcctg cggcgcggtg tggctgcggc gccggtggcc ggtcggcctc gccgtggcgt
     9841 tggtgccggt cgccttcctg tccaacaccg cgggcggcgc gggcgtggtc gccctcttca
     9901 cgctcgccgt gcaccggccc ctccggtacg tggcctgggc gggcggggcg tccgtggcgc
     9961 tggtcccggt cttcttctgg ctgcggcccg accccgacgt gccgtacgtc gtggcggtcg
    10021 cctcgggcgt gctgctcacc gcagcgatgg tcggctgggg catgttcgta cggtccaaac
    10081 ggcagctcgt gctgagcctg cgcgaccgcg cccggcacgc ggagaccgag gcccggctgc
    10141 gggccgagca ggcccagcgg ctggcccgcg aggccatcgc ccgcgagatg cacgacgtgc
    10201 tcgcccaccg gctcaccctg ctgagcgtgc acgcgggcgc cctggagttc cggccggacg
    10261 cgccgcgcga ggaggtcgcg cgggccgccg gggtgatccg ggagagcgcc cacgaggccc
    10321 tgcaggacct gcgggagatc atcggggtgc tgcgggccgg cgaggccgac gagccgcccg
    10381 gcgggcgccc gcagccgacg ctggccgcac tggacgccct cgtcgccgag tgccgcgagg
    10441 ccggcatgaa cgtcgccctc gaccaccgcg tcgccgaccc ggccgccgtc cctgcctccg
    10501 tcggccgcac cgcctaccgc atcgcccagg aggccctgac caacgcccgt aaacacgccc
    10561 ccggcaccga cgtcaccgtc cgtctcgccg gcacccccgg cgacggcctc acggtcaccg
    10621 tcaccaaccc cgccccgtcc tccgaggtac cgcccgtccc cggctccggc cagggcctga
    10681 tcggcctcac cgaacgcgcc tcgctggccg ggggacacct ggaacacggc acgcgaccgg
    10741 acggcggttt cgaggtacgg gggtggctgc cgtggccgcg cacctgagcc ccacggccgc
    10801 cgcccaccgc cccgcgcccc acacgcggcg cccggccggc acacgggcgc cctcgctcca
    10861 ggcagcctgc cgccccgact ccccacccgt gattacgtaa ccccatgact gcgacgccga
    10921 tcagactgct gctcgtggac gacgaccccc tcgtacgcgc cggactggcg ctgatgatcg
    10981 gcggggccga
//
