LOCUS       NZ_CP071839            11317 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6731521
            Orig. end                         :: 6742838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11317
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="23"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11317
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11317
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6317](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6317)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            476..1207
                     /gene="hemQ"
                     /locus_tag="S1361_RS30220"
                     /old_locus_tag="S1361_30205"
     CDS             476..1207
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="S1361_RS30220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30205"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_208035052.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPADR
                     AGYADEVQELFDQLAAKDVTVRGTYDLSGLRADADLMIWWHAETSDQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVR
                     EEIPFFTGRRKDIGELVADLA"
     gene            complement(1241..2899)
                     /locus_tag="S1361_RS30225"
                     /old_locus_tag="S1361_30210"
     CDS             complement(1241..2899)
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-483). E-value:
                     8.7e-20. Score: 63.3. Matches aSDomain:
                     nrpspksdomains_S1361_RS30225_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="S1361_RS30225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30210"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_208035053.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-23, bitscore: 82.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAAALCSAAGSLLLTSLAAAPAGGAPGTPGGAGAPGTAELRGTA
                     VAVARARAAGVDFGPCAAADLPEPPADVRCGTVTVPLDYARPDGRQIRLTVSRAPATQK
                     DPHNSKRKVPRQGALVYNPGGPGASGMYFPLIGAVPEWKRIAAAYDLIGYAPRGVGRSA
                     PLSCEDPARFFKAPTASPVHPSEAYKRQRVDQAKAYARGCVQRSGTAIRFYNSLNNARD
                     LDVLRAALGEDRLTFVGASYGSYFGSLYAAMFPSHVRRMVLDSAVNPDPQKIWYRSNLD
                     QSAAFEERWADFRDWIARHHDVYGLGATPAAVQRSYDTARERLTAKAAGGKVGPGQLQK
                     AFLTAGYYDDFWPGRAEALSAYLHGDPGPLVRLAAPSPETAAEAENGSAVYTAVECNDA
                     PWPTDFAVWDRDNTRLARVAPFETWDNAWMNLPCAYWPVSRQQPLDVRTGPGELPPVLI
                     LAAERDAAAPYQGALETNRRLAGSVLVTERDAGTHGIAGGPNRCVNGHLEAYLLEGRVP
                     ARHASCAPRPEPPASGGPADEGGRRDALKGKGERR"
     aSDomain        complement(1451..2548)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_S1361_RS30225_Abhydrolase_1.1"
                     /evalue="8.70E-20"
                     /label="S1361_RS30225_Abhydrolase_1.1"
                     /locus_tag="S1361_RS30225"
                     /protein_end="483"
                     /protein_start="117"
                     /score="63.3"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGMYFPLIGAVPEWKRIAAAYDLIGYAPRGVGRS
                     APLSCEDPARFFKAPTASPVHPSEAYKRQRVDQAKAYARGCVQRSGTAIRFYNSLNNAR
                     DLDVLRAALGEDRLTFVGASYGSYFGSLYAAMFPSHVRRMVLDSAVNPDPQKIWYRSNL
                     DQSAAFEERWADFRDWIARHHDVYGLGATPAAVQRSYDTARERLTAKAAGGKVGPGQLQ
                     KAFLTAGYYDDFWPGRAEALSAYLHGDPGPLVRLAAPSPETAAEAENGSAVYTAVECND
                     APWPTDFAVWDRDNTRLARVAPFETWDNAWMNLPCAYWPVSRQQPLDVRTGPGELPPVL
                     ILAAERDAAAPYQGALETNRRLAGSV"
     gene            complement(3046..3825)
                     /locus_tag="S1361_RS30230"
                     /old_locus_tag="S1361_30215"
     CDS             complement(3046..3825)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="S1361_RS30230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30215"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_208035054.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGSACTRLCLAAVRAAAVDAPGAPADGERGLT
                     LYEAAFLSGGPARVADVTLVSMARQRRLLLAHTGWATVVDPWGRDEMEQSVIGAIGPEG
                     QSRIAPVRARAAGAEAVRRLADGLVRAGLAVPEGAATTVGAAVRQVRAAALAVALLGVT
                     ALLLPIETGTPRPLVGAWFALPLTLTLSCLAIARFEVHPYSRWASPAGQRLLSALAGRP
                     AGDERSFLTSVAVRGVRAIGEPELRAAFTHRDQPWRE"
     misc_feature    3885..3902
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     gene            complement(3942..4712)
                     /locus_tag="S1361_RS30235"
                     /old_locus_tag="S1361_30220"
     CDS             complement(3942..4712)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997027.1"
                     /locus_tag="S1361_RS30235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30220"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_208035055.1"
                     /transl_table=11
                     /translation="MRPRPPINGRGIFSGTGLVITALAATVVALLVPLWSYAGRQDPAS
                     ANVLSAQTVTTPYGPLSAQDRDFVTKVRLAGLWELPAGELAQRKGTTPAVRTAGQHLVD
                     GHTSLDAHVRTVATQLGLALPNEPNEQQKQWLAALNAADGQDFDRRFAGLLRLAHGRVF
                     SLVAQVRAGTQNSLVRDLADDANATVLDHIKVLEATGYVDFAVLAEDLAASATPVPTPP
                     QVDPSAAVPVTPSPGETYSLPPAAASPPPATSSP"
     gene            complement(5001..6317)
                     /locus_tag="S1361_RS30240"
                     /old_locus_tag="S1361_30225"
     CDS             complement(5001..6317)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675658.1"
                     /locus_tag="S1361_RS30240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30225"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_208035056.1"
                     /sec_met_domain="DUF692 (E-value: 2.5e-96, bitscore: 320.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVEAMPGIDWVEVVAENVCPGHLPESLLR
                     LRERGVTVVPHGVSLGLGGADHPDEGRLTALAERAEALGSPLVTEHIAFVRAGGALTAS
                     PGLEAGHLLPVPRTRDALDVLCENVRIAQEALPVPLALENIAALFSWPDEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAEALAALPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPGPVLDILTGLAARVAPPGVLLERDENFPGPGELERELDAVREAVAAGRGRAAEP
                     AVGGTAVRGAVGAGARGVGVLARPVAGARERVAVAQTAVLSALVAGTPVPEGFDRVRMG
                     VQARALAAKRAGVVAKVAPELPVILGAGYREAFLEYARQRPMRGGYRQDALDFAGHLLK
                     LGRAGDAGSLRELREWWLDRSGPAPRKRGFAARVLRRRA"
     gene            complement(6440..7159)
                     /locus_tag="S1361_RS30245"
                     /old_locus_tag="S1361_30230"
     CDS             complement(6440..7159)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675659.1"
                     /locus_tag="S1361_RS30245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30230"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_208035057.1"
                     /transl_table=11
                     /translation="MSHVPAPTADSPFRSARSPRDEAPQFVLPLVVRIERSAPPARTDA
                     LETAARAVLVLLGDDRTQGDGEWAEAVRNWEDARIRKVVRRARGAEWRRAGTLPGITVT
                     GSSAEVRVFPPIPLDGWPKDLARLQVSGTELDDPEPPLPADPAAGPVLWLNPELEMSAG
                     KAMAQAGHGAQLAWWALSPAERTAWRDAGHPLAVRTADPADWPRLTAGGLPVVRDAGFT
                     EIAPGSCTVVADHPALR"
     gene            7292..7732
                     /locus_tag="S1361_RS30250"
                     /old_locus_tag="S1361_30235"
     CDS             7292..7732
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675660.1"
                     /locus_tag="S1361_RS30250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30235"
                     /product="DUF6086 family protein"
                     /protein_id="WP_208035058.1"
                     /transl_table=11
                     /translation="MSQYFDMGDETLWNPSNGASRLFQRQVEVFEAELELPSGIGPMRN
                     DECQIDPAVFGAFVHALLARHRRTGHTVVLALSEGFVGTVVALAERAGIDIDWGRLEAF
                     PDGPLSDVQVSATGVSAPPEDGGPWAAAVRARAAELGRGMAR"
     gene            7807..8685
                     /locus_tag="S1361_RS30255"
                     /old_locus_tag="S1361_30240"
     CDS             7807..8685
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675661.1"
                     /locus_tag="S1361_RS30255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30240"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_208035059.1"
                     /transl_table=11
                     /translation="MDSDLRLTAVGRRYGIRGPWVLRGVDLVIAPGTLTRIEGANGTGK
                     STLLRLLAGLDAPSEGRITGRPRTAYVPERFPSALPFTAAGYLTHLGTVHGLGRRAAAR
                     AAATWLERFGAGAHAGTPMARLSKGSSQKVAVAQALLGDPELLVLDEAWTGLDTAARGE
                     LERAVAERAAAGGAVVFVDHDPRRLAGAPDATYAVTGGGLERRTGDRTRPAGPVTLVTV
                     QGAPGGQLPPDAVRVAVSAEETAPGSHRLAVPASHSDILLRALLTARPPWHVVRVEPPA
                     SPPSADPGSCP"
     gene            8682..9353
                     /locus_tag="S1361_RS30260"
                     /old_locus_tag="S1361_30245"
     CDS             8682..9353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="S1361_RS30260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30245"
                     /product="ABC transporter"
                     /protein_id="WP_208035060.1"
                     /transl_table=11
                     /translation="MTALLRYHADLLLRSQRWLPPVILYVAFLGVGVQSGQPVLDSLGY
                     TAAGLLPVAAWLVRICVTGEPPAARACVAAARGPVRAHLACLLTALLVAALLGVAATVV
                     VTLVSDPASNGHRIHVPRLRAAAAGLPAVLACALLGTAVGALTNRPVLRSTGRAVSAML
                     LGALLAVVSAGSPAQAAVSGLVTGSQTGRAPVPLLPLAAAALLTAAAFAATGRLVSRRS
                     P"
     gene            complement(9345..9527)
                     /locus_tag="S1361_RS30265"
                     /old_locus_tag="S1361_30250"
     CDS             complement(9345..9527)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="S1361_RS30265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30250"
                     /product="hypothetical protein"
                     /protein_id="WP_208035061.1"
                     /transl_table=11
                     /translation="MSDEDYCTIVETVKPAKADGPPYADCVLCREPTEYPESYKGITLC
                     PVCEWQEAQRTACSG"
     gene            complement(9545..10369)
                     /locus_tag="S1361_RS30270"
                     /old_locus_tag="S1361_30255"
     CDS             complement(9545..10369)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675664.1"
                     /locus_tag="S1361_RS30270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_30255"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_208035062.1"
                     /transl_table=11
                     /translation="MIPHVRSITVVCVLGAALTACGAAGSGRPSASSSPAGPPVLAPGP
                     GGLTPVFEHGPRTRGKTVALTFDADMTADQGPRAASGEHFDNPGLIGALRELKVPATVF
                     MTGRWADQYPAQARDLGRDPQFEVANHSWSHYAFTADCYGLPTVDADGMRADVQRAYTS
                     LRKAGVPKAMPYFRFPGGCYDQRALRALSGLGVTAVQWDVVSGDAFATDADAVVRQVLD
                     GVKPGSVVVMHCTRSAAPTTEQVVRRVVPELRERGYRFVKVSELIGQAGTGR"
     misc_feature    complement(10378..10398)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.03"
                     /tool="antismash"
     misc_feature    complement(11168..11183)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.2"
                     /tool="antismash"
ORIGIN
        1 tgccgcccgt cgacggccgc accatcaagg cctccacctt cgcctcccag aagtggggct
       61 ggatcgccga ggaggacccg gaggtggtgg tgctgcgcac ctccgtgggc cgctacggcg
      121 agacggaggt gctcggccgg gacgacgccg gcctggtgga ggtctcccgg cacgacctgc
      181 gggaggccac cggcctggac gccgtgccgc tggagacccg ggtcacccgc tgggccgacg
      241 gcctgcccca gtaccccgtc gggcaccacg cgcgcgtggc ccgcatccgc gagcaggtcg
      301 ccaaggtccc gggtctcgcg gtgtgcggcg ccccgtacga cggcgtcggc atccccgcgt
      361 gcatcgcgag cgcccacgcc gccgtggacc agctggccgg tgatccgtca ggggtggcgg
      421 agctgacggc caacccggtg cagagcctgc acggcggagc gggagaatag ggaccatgag
      481 tgacgacgcc cccaccaccg agtccggcag gatcccgaac aagggcaagc tggccaagga
      541 cctcaacgag gtcatccgct acaccctctg gtcggtcttc aagctgaagg acgtgctgcc
      601 ggcggaccgc gcgggctacg ccgacgaggt ccaggagctg ttcgaccagc tcgccgcgaa
      661 ggacgtgacg gtccgcggca cctacgacct ctccggtctg cgcgccgacg ccgacctgat
      721 gatctggtgg cacgccgaga ccagcgacca gctccaggag gcgtacaacc tcttccgccg
      781 caccaggctg ggccgtgcgc tggagccggt ctggtcgaac atggcgctgc accgcccggc
      841 ggagttcaac cgctcgcaca tcccggcgtt cctggccgac gagacgcccc ggaactacgt
      901 gagcgtctac cccttcgtgc gctcctacga ctggtacctg ctgcccgacg aggaccgccg
      961 ccgcatgctc gccgaccacg gcaagatggc ccgtggcttc cccgacgtgc gcgccaacac
     1021 ggtcgcctcg ttctccctcg gcgactacga gtggatcctg gccttcgagg ccgacgagct
     1081 gtaccgcatc gtcgacctca tgcgtcacct gcgcggctcc gaggcccgga tgcacgtccg
     1141 cgaggagatc ccgttcttca cgggccgccg caaggacatc ggcgaactgg tggcggacct
     1201 cgcctgacag cgccccgaag gggcccggcc acgggggtgc ctagcgccgc tcgcccttgc
     1261 ccttgagcgc gtcccggcga cccccctcgt cggccggacc gccggaggcc ggcggttcgg
     1321 ggcgcggcgc gcacgacgcg tgccgcgccg gaacgcggcc ctccagcagg tacgcctcca
     1381 ggtgcccgtt gacgcacctg ttgggcccgc ccgcgatgcc gtgcgtgccc gcgtcccgct
     1441 cggtcaccag caccgagccg gccagccgcc ggttcgtctc cagggccccc tggtacggcg
     1501 cggcggcgtc ccgctcggcg gccaggatca gcaccggcgg cagctcaccg ggcccggtcc
     1561 gcacgtccag cggctgctgc cgggacaccg gccagtaggc gcacggcagg ttcatccacg
     1621 cgttgtccca ggtctcgaag ggcgccaccc gggcgagccg ggtgttgtcg cggtcccaca
     1681 ccgcgaagtc cgtcggccac ggcgcgtcgt tgcactccac ggccgtgtac accgcgctgc
     1741 cgttctccgc ctcggcggcc gtctccgggc tcggcgcggc cagccggacc agcggacccg
     1801 ggtcgccgtg cagatacgcc gacagggcct cggcccggcc cggccagaag tcgtcgtagt
     1861 acccggccgt caggaaggcc ttctgcaact ggccggggcc gaccttgccg cccgccgcct
     1921 tcgcggtcag ccgctcgcgc gcggtgtcgt agctgcgctg caccgccgcg ggcgtggcgc
     1981 cgagcccgta cacgtcgtgg tgccgggcga tccagtcccg gaagtccgcc cagcgctcct
     2041 cgaacgccgc cgactggtcc aggttgctgc ggtaccagat cttctgcggg tccgggttca
     2101 ccgccgagtc cagcaccatg cggcgcacgt gcgaggggaa catcgccgcg tacagcgagc
     2161 cgaagtagct gccgtacgac gccccgacga aggtcagccg gtcctcgccc agcgcggccc
     2221 gcagcacgtc caggtcccgg gcgttgttga gcgagttgta gaagcggatc gcggtcccgg
     2281 agcgctggac gcagccgcgc gcgtacgcct tcgcctggtc gacccgctgc cgcttgtacg
     2341 cctccgacgg atgcaccggg gacgcggtgg gtgccttgaa gaagcgcgcg gggtcctcgc
     2401 aggacagcgg cgcggaacgg cccaccccgc gcggggcgta gccgatcagg tcgtaggcgg
     2461 cggcgatccg cttccactcc gggaccgcgc cgatcagcgg gaagtacatg ccggaggcgc
     2521 ccgggccgcc cgggttgtag accagggcgc cctgccgggg aaccttccgc ttgctgttgt
     2581 gcggatcctt ctgagtggcc ggggcccggc tgaccgtcag ccggatctgc cggccgtccg
     2641 ggcgggcgta gtccagcggg accgtcaccg tgccgcaccg cacatcggcc ggcggctccg
     2701 gcaggtcggc ggcggcgcac ggaccgaagt cgaccccggc cgcccgggca cgcgccacgg
     2761 cgaccgcggt gccgcgcagc tcggccgtgc cgggggcacc cgcgccgccc ggggtgccgg
     2821 gtgcgccgcc cgccggggcc gcggccagtg aggtcaggag caaggaaccg gcggccgagc
     2881 agagagcggc agctttcatc gcgtatccct tcggtgcggc ggcgatggca cggctgccac
     2941 ggcgcggcga ccacacaagg gatgtttcgt ccggctgtgg aggaaggcaa gcaccacccg
     3001 gccggtgtcg gcgtcaatgc cccccgtgcg cccccggaag ccgcctcact cccgccaggg
     3061 ctggtcgcgg tgggtgaagg ccgcgcgcag ttccggttcg ccgatggcgc ggacgccgcg
     3121 tacggcgacg gaggtgagga acgaccgctc gtcgccggcc ggcctgccgg ccagcgcgct
     3181 cagcagccgc tgcccggccg gggaggccca gcgcgagtac gggtgcacct cgaaccgggc
     3241 gatggccagg cagctgagcg tcagcgtgag gggcagggcg aaccaggcgc ccaccagcgg
     3301 gcgcggtgtg ccggtctcga tgggcagcag cagcgcggtc acgcccagca gggcgacggc
     3361 cagtgcggcg gcccgcacct ggcggacggc ggcacccacg gtcgtggccg cgccctccgg
     3421 caccgcgaga ccggcgcgca ccagcccgtc cgcgagccgc cgtacggcct ccgcgccggc
     3481 cgcccgcgcc cgtaccggag cgatccgcga ctgtccctcg ggccctatgg cccctatgac
     3541 ggactgctcc atctcgtcac ggccccacgg gtccacgacc gtggcccagc cggtgtgcgc
     3601 gagcagcagc cggcgctggc gggccatgga gacgagcgtc acatcggcga cccgtgcggg
     3661 gccgccggag aggaaggccg cctcgtacag cgtgagaccg cgctccccgt ccgccggcgc
     3721 accgggtgcg tccacggctg ccgcgcggac ggccgccagg cacagccggg tgcaggcgga
     3781 cccggcgaaa gcccaggcca ggagcaggag aaggacccag aacatgacgt gtttgtatgc
     3841 cagaggactg ggcgaacgcc atgccctttc atcatccgga cggactgttg ctggtatgtg
     3901 acgttccgtt tcccgggctg gtccgtggcc gtccggggcg gtcaggggct gctcgtcgcc
     3961 ggcggcgggc tcgcggcggc gggcggcagg gagtaggtct cgcccggcga cggcgtgacc
     4021 ggtacggcgg cactcgggtc gacctgcggc ggggtcggca ccggggtcgc ggaggccgcc
     4081 aggtcctcgg ccagtacggc gaagtcgacg taccccgtcg cctccaggac cttgatgtgg
     4141 tccagcaccg tcgcgttggc gtcgtcggca agatcacgga ccagggagtt ctgggtgccg
     4201 gcccggacct gcgcgaccag ggagaacacc cggccgtgcg cgagccgcag caggccggcg
     4261 aaccggcggt cgaagtcctg cccgtcggcc gcgttcaggg cggcgagcca ctgcttctgc
     4321 tgctcgttcg gctcgttggg cagggccagg ccgagctggg tggcgaccgt gcggacgtgc
     4381 gcgtccaggg aggtgtggcc gtcgacgagg tgctgcccgg ccgtgcggac ggccggtgtc
     4441 gtccccttgc gctgcgccag ctccccggcg ggcagctccc acagcccggc cagccggacc
     4501 ttggtgacga agtcccggtc ctgcgccgac aacgggccgt acggcgtcgt caccgtctgg
     4561 gcgctcagca cgttcgcgct cgccgggtcc tgccggccgg cgtacgacca gagcgggacc
     4621 agcagcgcga cgaccgtggc cgccagggcg gtgatgacga gaccggtgcc gctgaatatg
     4681 ccacggccgt tgatcggtgg acgcggtcgc atggtgcctc ctgcaccgca cgccggggat
     4741 gtgctgggga tgggtgcggg gttggcatat tactggggga cgggggccgg gttccgcgcg
     4801 cgggtgggcg gtggccgttc gcgggccggc ggggcggttg cggtcccgtc ggcgaccacc
     4861 ggtgcaccgt ggccggtcgc gcggttcgcc gtgcccccgg tgaaccgcag tgcgtcggcc
     4921 gtggccgtgc ccgcgcggcc tgcgggccgc ggacggacgg caccccgtcg gcgcggacga
     4981 ccaccggccc gggcactccc tcaggcccgg cgccgcagca cccgggccgc gaagccccgc
     5041 ttgcggggcg ccgggcccga gcggtccagc caccactcgc gcagctcccg gagggacccc
     5101 gcgtcccccg cccgccccag cttcagcagg tgcccggcga agtccagcgc gtcctgccgg
     5161 tacccgccgc gcatcggccg ctgccgcgcg tactccagga acgcctcccg gtaccccgcc
     5221 cccaggatca ccggcagctc gggcgccacc ttggccacca cccccgcccg cttggccgcg
     5281 agcgcccgcg cctgcacccc catccgcacc cggtcgaacc cctccggcac cggtgtcccc
     5341 gccaccaggg cggacagcac ggcggtctgg gccaccgcca cccgctcgcg cgccccggcc
     5401 accggccgcg ccagcacgcc gacgcccctg gccccggcac cgacggcccc cctcaccgcc
     5461 gtacccccca ccgcaggctc cgccgcgcgc ccgcgcccgg ccgccaccgc ctcccggacg
     5521 gcgtccagct cccgctccag ctcgcccggt ccggggaagt tctcgtcccg ctccaggagc
     5581 accccgggcg gcgccacccg ggcggcgaga ccggtcagga tgtcgaggac cgggcccggc
     5641 acggggtgcg cgtggctgtc gtgccagacg ccgtcgcgct cgaagccgcc cgcgacatgg
     5701 acgtaggcga tcgcctccag gggcagcgcg gccagcgcct cggccgggtc ctcgccccgg
     5761 ttgacgtggt tggtgtgcag gttggccacg tcgatcagca gccgtacgcc ggtccgctcg
     5821 accagctcgg acaggaactg cccctcggtc agctcctcgt ccggccagga gaacagcgcg
     5881 gcgatgttct ccagcgccag cggcaccggc agcgcctcct gggcgatacg gacgttctcg
     5941 cacagcacgt ccagggcgtc ccgggtccgc ggcacgggca gcaggtggcc ggcctccaga
     6001 cccggggagg cggtgagcgc gccgcccgcc cggacgaagg cgatgtgctc ggtgaccagc
     6061 ggcgagccga gggcctccgc ccgctcggcg agcgccgtca gccgcccctc gtcggggtgg
     6121 tcggcaccgc cgaggccgag ggagacaccg tgcgggacca ccgtcacacc gcgctcgcgc
     6181 agccgcagca gcgactcggg aaggtggccg gggcagacgt tctccgccac gacctccacc
     6241 cagtcgatgc ccggcatcgc ctccacagcg tccgcgatct ccggccgcca cccgatcccg
     6301 gtccccagtc gccgcatgcc gcttcccccc tcctcgcccc gcgctcctgc gccgagtgac
     6361 gggggtatgg ccccgtcggc cgcccgcgaa cccccaccct gacgcgttca gagcaacatt
     6421 tgaggttccg gccgaccgcc tagcgcagcg cggggtgatc ggcgacgacc gtgcaggacc
     6481 ccggagcgat ctccgtgaag ccggcgtccc gcaccaccgg caggccgccg gcggtgagcc
     6541 ggggccagtc ggccgggtcg gccgtgcgga cggcgagtgg gtggcccgcg tcgcgccacg
     6601 cggtgcgctc cgcgggggac agggcccacc aggccagttg ggccccgtgg ccggcctggg
     6661 ccatcgcctt gccggccgac atctccagct ccgggttgag ccagagcacc ggcccggcgg
     6721 ccgggtcggc ggggaggggc ggctcggggt cgtcgagttc ggtgcccgac acctggagcc
     6781 tggccaggtc cttgggccag ccgtccaggg ggatcggggg gaagacgcgg acctccgcgg
     6841 agctgccggt caccgtgatc ccgggcagtg tcccggcgcg ccgccactcg gcgccgcggg
     6901 cccgccggac caccttgcgg atgcgggcgt cctcccagtt ccgcacggcc tcggcccact
     6961 cgccgtcgcc ctgcgtccgg tcgtcgccga gcaggacgag cacggcccgg gccgccgtct
     7021 ccagcgcgtc ggtgcgggca ggcggtgcgc tgcgctcgat gcgtacgacg agcggcagca
     7081 cgaactgggg tgcctcgtcg cgggggctgc gtgcggaccg gaagggactg tcggcggtgg
     7141 gggctggaac gtggctcacg ggcgcccagc ctacgaagcc gcgaccgcac cgatcaacgc
     7201 gcggggcgcg cgcgggaaat tccggcgacg ccggtgaacc ggccggcacg ctcggccctc
     7261 ttgaccgagt gagcggacgt gagggggcgc ggtgagccag tacttcgaca tgggggacga
     7321 gacgctgtgg aatccgtcca acggggcgtc gcggctcttc cagcggcagg tggaggtctt
     7381 cgaggccgaa ctggagctgc cgtccggcat cgggccgatg cggaacgatg agtgccagat
     7441 cgatccggct gtcttcgggg ccttcgtgca cgccctgctc gcccggcacc ggcggaccgg
     7501 ccacaccgtc gtcctggccc tctccgaagg cttcgtcggc accgtcgtcg ccctcgccga
     7561 gcgcgccggg atcgacatcg actggggccg gctcgaagcc ttcccggacg gccccctgtc
     7621 cgacgtgcag gtctcggcca ccggggtgtc cgccccgccc gaggacggcg gaccctgggc
     7681 tgcggcggtg cgtgccaggg cggccgaact gggccggggc atggcccgtt gagcggcgcc
     7741 cggccgcggc cgccgtaccg gaacggcccc ctgcacccga cccccccggc ccacacaatg
     7801 acctccatgg acagtgacct gcgcctgacc gccgtgggcc gccgttacgg catccgcggc
     7861 ccctgggtgc tgcggggtgt cgacctcgtc atagcccccg gcaccctcac ccggatcgaa
     7921 ggggcgaacg gcaccgggaa gtccactctg ctgcggctgc tcgcggggct cgacgcgccc
     7981 agcgagggcc ggatcaccgg ccgcccgcgc accgcgtacg tgccggagcg gttcccgtcc
     8041 gccctgccct tcacggccgc cggctacctg acccacctcg gcaccgtcca cggcctcggc
     8101 cgaagggccg ccgcacgcgc cgcggccacc tggctggagc ggttcggcgc gggcgcgcac
     8161 gccggtacgc cgatggcgcg gctgtccaag ggcagcagcc agaaggtcgc cgtggcccag
     8221 gccctgctgg gcgacccgga actgctggtg ctggacgagg cctggaccgg cctcgacacg
     8281 gcggcgcggg gcgagctgga gcgggcggtg gccgagcggg cggccgccgg gggtgccgtg
     8341 gtcttcgtgg accacgaccc gcgccggctc gccggggcgc cggacgcgac ctacgcggtg
     8401 accggcggag gcctggaacg ccgtaccggc gaccggaccc ggccggccgg accggtcacg
     8461 ctcgtcaccg tgcagggggc gccgggcggg cagctgccgc cggacgcggt gcgggtcgcc
     8521 gtctccgccg aggagacggc cccgggcagc caccggctcg ccgtgccggc ctcccactcc
     8581 gacatcctgc tgcgcgccct gctcacggcc cgcccgccct ggcacgtggt gcgcgtcgaa
     8641 ccgcccgcca gtccgccgtc cgccgacccc ggaagctgcc catgaccgcg ctgctgcgct
     8701 accacgccga cctgctgctg cgctcccagc gctggctgcc cccggtgatc ctgtacgtcg
     8761 cgttcctcgg cgtcggcgtg cagagcggcc agccggtgct ggactcgctc ggttacacgg
     8821 ccgccggtct gctcccggtg gccgcctggc tggtacggat ctgtgtcacc ggcgagccgc
     8881 ccgcggcccg ggcctgcgtc gccgctgccc gcggccccgt ccgggcacac ctcgcctgtc
     8941 tgctcaccgc actgctcgtc gccgcactgc tcggggtggc ggccaccgtg gtggtgacgc
     9001 tggtcagcga cccggcgagc aacggccacc ggatccacgt cccccggctc cgcgccgccg
     9061 ccgcgggact gcccgccgtc ctcgcctgcg ccctgctggg tacggccgtc ggcgcgctca
     9121 ccaaccggcc ggtgctgcgc tccaccggcc gcgcggtgtc ggcgatgctg ctgggcgcgc
     9181 tgctggcggt ggtgtcggcc ggttccccgg cgcaggcggc ggtcagcggc ctggtgaccg
     9241 gctcgcagac cggccgggca ccggtgccgc tgctgccgtt ggcggccgcg gccctgctca
     9301 ccgcggccgc gttcgccgcg accggccggc tcgtctcgcg ccgctcaccc tgagcaggcc
     9361 gtgcgctggg cctcctgcca ctcgcagacc gggcacagcg tgatgccctt gtacgactcg
     9421 gggtactccg tcggctcccg gcacagcacg cagtccgcgt acggcggacc gtcggccttc
     9481 gccggcttca ccgtctcgac gatcgtgcag tagtcctcgt cgctcatgcc ttcagcgtag
     9541 gacgtcagcg gcccgtgccc gcctggccga tcagttccga gaccttcacg aaccggtagc
     9601 cgcgctcccg cagttccggt acgaccctgc gcacgacctg ctcggtggtc ggggcggcgc
     9661 tgcgggtgca gtgcatgacg accaccgacc ccggcttgac cccgtcgagc acctgtctga
     9721 ccaccgcgtc ggcgtccgtg gcgaaggcgt ccccgctgac cacgtcccac tgcacggcgg
     9781 tcaccccgag cccgctcagc gcgcgcagcg cccgctgatc gtaacagccg ccggggaagc
     9841 ggaagtaggg catcgccttc ggcacgcccg ccttgcgcag ggaggtgtag gcgcgctgga
     9901 cgtcggcccg catcccgtcg gcgtcgacgg tcggcaggcc gtagcagtcg gcggtgaagg
     9961 cgtagtggct ccaggagtgg ttggccacct cgaactgcgg gtcccggccc aggtcgcggg
    10021 cctgggccgg gtactggtcg gcccaccggc cggtcatgaa cacggtcgcc ggcaccttca
    10081 gctcacgcag ggcgccgatc agaccggggt tgtcgaagtg ctcgccggag gccgcgcggg
    10141 gcccctggtc ggcggtcatg tcggcgtcga aggtgagcgc gaccgtcttg ccccgggtgc
    10201 gcggaccgtg ctcgaagacc ggggtcagcc cgccggggcc cggcgcgagc acgggcggac
    10261 cggctgggga cgaggaggcg gacgggcgcc cactgcccgc agcgccgcag gcggtgagcg
    10321 ccgcgcccag gacgcagacg acggtgatgg agcgtacgtg tgggatcacc gtgcgaacat
    10381 aaagctgaat gtctgtttat gtgattattt cggcccgcga cacggcggta cggtcacccg
    10441 cgcggcggcg gcgacccgcg ctcccgtcac tccagttcgc gccgctccag gggtttgacg
    10501 gccggtccct ggagcaccgt gccgtcgacg gcgaagcgcg agccgtggca ggggcactcc
    10561 caggcgcgtt cggcgcggtt gaaggagacc aggcagccca ggtgggtgca gcgggccgac
    10621 acggcgtgca gggcgcccgc ctcgtcgcgg tggaccgcga gccgggcgga gccggagcgg
    10681 accaccgcgc cgtcgcccgg ggccaggtcg gccaccgagg ccggcggctt cagccggtcg
    10741 ccgacgaagt gccgggccac ctcggcctgc gtcttcagca gcgacggcgc ctcgcgcacc
    10801 accgacttca gccggcgcgg gtcgtacagc tcccgccacg cgcagtcccg gccggtgatc
    10861 agcgcggtca gcagcagccc gcccatgatc ccgccgctca ggccccagcc gccgaagccg
    10921 gtgacgacgt aggcgtgccg ggcacccggg tgcagcgggc cgaccagcgg gacggtgtcg
    10981 gtggactcgt tgtcctgggt cgcccaggca tggtccaggg tcacccccgg gaagtgcacc
    11041 tccgcccagc cggcgagccg gtcgaagccg gcctgcggat cgccggtgcc gggcgtgaag
    11101 tgctccccgg tgaccacgag cagccgctgc ccgtccgggc catagggcgc cgtacgcacc
    11161 gagcgggtgt tctcgtccgg cgtgatgtac atcccgtgcg gatccggtcc gtcgcccagc
    11221 ggaccggcga ccaccagctc ccggcgcggg gacagccggg cgaacagcag ggcccggtcg
    11281 aacaccgggt agtgggtggc gacgacgaca tcccggg
//
