LOCUS       NZ_CP071839            10210 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4669397
            Orig. end                         :: 4679607
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10210
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10210
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10210
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5210](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5210
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            512..1090
                     /locus_tag="S1361_RS20970"
                     /old_locus_tag="S1361_20915"
     CDS             512..1090
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /locus_tag="S1361_RS20970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20915"
                     /product="VTT domain-containing protein"
                     /protein_id="WP_208033346.1"
                     /transl_table=11
                     /translation="MDGTHWMYVLLVLVTMPPMIPNSALVAGAGALAAAGSLNLPLLIV
                     ILLASTVLGDMSMFWAGHLARSRALRWMSRRQKRRSMLDWAAERFRRYGVPSVIVMRFV
                     PSGRGLGGVTAGIVDFPLRSYLLGALLAEAVFVSCTLGLGYLGGRLVDNSVTVVCMGPA
                     VSVLTAGSVMAVRWAWTRWSPRRAGDRGD"
     misc_feature    650..652
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1173..1511
                     /locus_tag="S1361_RS20975"
                     /old_locus_tag="S1361_20920"
     CDS             1173..1511
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525874.1"
                     /locus_tag="S1361_RS20975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20920"
                     /product="ArsR family transcriptional regulator"
                     /protein_id="WP_208036700.1"
                     /transl_table=11
                     /translation="MPADRLARVLGTLANPHRLGVVAALARRRNYVSRLARELGISRAL
                     LQAHLRRLEAAGLVTGQLEISSDGKAMKFYEVTPFALHLTPQSIADALSPLTIHAHVPE
                     DGEKGSPQ"
     gene            1508..1777
                     /locus_tag="S1361_RS20980"
                     /old_locus_tag="S1361_20925"
     CDS             1508..1777
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="S1361_RS20980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="S1361_20925"
                     /product="hypothetical protein"
                     /protein_id="WP_208033347.1"
                     /transl_table=11
                     /translation="MSYHGWQEVVGTVGLVGAFASVVTIVIWHMAMTWRARAVLARDHQ
                     YRTLAEQATRSHDSAVQQLAGIDERLADLQTRMRSVERILKEVE"
     gene            1894..4167
                     /locus_tag="S1361_RS20985"
                     /old_locus_tag="S1361_20930"
     CDS             1894..4167
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180855.1"
                     /locus_tag="S1361_RS20985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20930"
                     /product="MMPL family transporter"
                     /protein_id="WP_208033348.1"
                     /transl_table=11
                     /translation="MPPKGKRLQLQNGTLIERVAGWSIRHRALAIGGWLALVVLAVLST
                     SLVSGDSAVTKDPGESGAAQQVLNEQKDWEPVLENVLVQSRDKDGPAFKDDPELQQAVK
                     DLVAEFGKTPGAVFRLRTPLDPQGEQQISADGRSGLVTFFVAGPNVKMDAHFDAVVDAV
                     HKVADRHKDIRVVQAGDQSLSRAVDDAVKKDFAGAESTSLPITLVILLIVFGALVAAAV
                     PLLLAATAVAAAFGLIGVFGQWVAVNSAVSSMILLIGVAVSIDYSLFYLRREREERAAG
                     RTVAEALRITAKTSGHVVAVSGVTVMLCVSGLLLSGLDVFRGLTLGTILIVGLAMTGSV
                     TVLPALLAALGHRVDKGRIPWLGKRRTTVLESRTWRRLAQKVVKRPLVWGGAAVVALVV
                     MALPMFSMHLQDAAVVNSLPRSAPTVDAAIRMQDAFPGSPTPARIAVWNADGGSPDTPA
                     VRAAVGRLHDEIAASDGRLFGPVSTVRVDDVLVVRVPLAGSGTDDDSHRALETLRDTAL
                     PRTLGKVDGVEYATAGRTAFAHDFAGRLSGRTFAVIAFVLVLAFVLLLLVFRSLTIPLV
                     SIALNLLSMGASYGVLTWVFQYGNLSSPLGFTSYGGVAEWLPLFMFVVLFGLSMDYHIF
                     VLSRIRERRQAGAEPRDAVVGGAAASAGVVTSAAVIMTAVFTIFITLSAIENKMMGVGM
                     AVAILIDATLVRGILLPAALALLGRRAWELPRGLRRLPGGTSGSAPAGPTGAVSRPAEG
                     VRRS"
     misc_feature    1915..1917
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5210
                     /locus_tag="S1361_RS20995"
                     /old_locus_tag="S1361_20935"
     CDS             5001..5210
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311933.1"
                     /locus_tag="S1361_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20935"
                     /product="hypothetical protein"
                     /protein_id="WP_208033349.1"
                     /sec_met_domain="strepbact (E-value: 8.2e-15, bitscore:
                     53.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSAPQVETAEISDAELDNVSGGLSPHAGLTAGPTTVGDADVLAA
                     LGTVQNQVLATAAQYDQVSVSVSL"
     gene            complement(5264..5551)
                     /locus_tag="S1361_RS21000"
                     /old_locus_tag="S1361_20940"
     CDS             complement(5264..5551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383478.1"
                     /locus_tag="S1361_RS21000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20940"
                     /product="SCO5918 family protein"
                     /protein_id="WP_208033350.1"
                     /transl_table=11
                     /translation="MRCVIARFPFDLVKSEVEQAMSGITPESVTGVSVTIGRRVYPVMQ
                     VGEVITRQNRRDFTVAEMRRALTRLGFTCHEPRPVVPALETRADEEALGW"
     gene            complement(5583..5918)
                     /locus_tag="S1361_RS21005"
                     /old_locus_tag="S1361_20945"
     CDS             complement(5583..5918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734848.1"
                     /locus_tag="S1361_RS21005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20945"
                     /product="CBS domain-containing protein"
                     /protein_id="WP_208036702.1"
                     /transl_table=11
                     /translation="MHAPGPQVDDHMAVDVALSVLIGARVAHLLLRDEDGRCAGLVTRA
                     QLTAHRGGSWYSDRTRLRDLPLDRGPFTSSRAALGEAEAAMRVRTLDVSPVIDEHGYAL
                     GVLALTP"
     gene            complement(6071..7648)
                     /locus_tag="S1361_RS21010"
                     /old_locus_tag="S1361_20950"
     CDS             complement(6071..7648)
                     /EC_number="3.6.4.-"
                     /GO_function="GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014673896.1"
                     /locus_tag="S1361_RS21010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20950"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_208033351.1"
                     /transl_table=11
                     /translation="MNRTPRRSGGASSGFRADRRSARSAGAAHGGRFRTQEPGRQGPRT
                     GTGRKNGPAAHSTPRRQEFALPTTFTEPLPAVESFAELDMPARLLAALGAEGVTVPFPI
                     QAATLPNALAGRDVLGRGRTGSGKTLAFGLPLLARLDGQRAQPRQPLALVLVPTRELAQ
                     QVTDALTPYARALRLRLATVVGGMSIGRQASALRGGAEVVVATPGRLKDLIERRDCRLD
                     EVCVTVLDEADQMADMGFMPQVTALLDQVPAGGQRLLFSATLDRNIDLLVRRYLHDPVV
                     HSVDPSAGAVTTMDHHLLHVRDDDKQTAATEIAARDGRVIMFLDTKHSVDRLTKHLLSV
                     GVRASALHGGKSQSQRNRTLAQFKDGHVTVLVATNVAARGIHVDGLDLVVNVDPPGDHK
                     DYLHRGGRTARAGESGTVVTLVLPHQRRAVDRLMTDAGIAANAARVRPGEPELQRITGA
                     RTPSGVPVVLTAPAEDRREGKGSSAGRRRRGGAGRGRSAGTGRGEGGQGAGRGGEARGR
                     RPAPRNNA"
     misc_feature    complement(7727..7742)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            complement(7897..8100)
                     /locus_tag="S1361_RS21015"
                     /old_locus_tag="S1361_20955"
     CDS             complement(7897..8100)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483357.1"
                     /locus_tag="S1361_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20955"
                     /product="cold-shock protein"
                     /protein_id="WP_007383480.1"
                     /transl_table=11
                     /translation="MASGTVKWFNAEKGFGFIEQDGGGADVFAHYSNIATSGFRELQEG
                     QKVTFDVTQGQKGPQAENIVPA"
     misc_feature    complement(8288..8301)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.65"
                     /tool="antismash"
     gene            complement(8332..8670)
                     /locus_tag="S1361_RS21020"
                     /old_locus_tag="S1361_20960"
     CDS             complement(8332..8670)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014673897.1"
                     /locus_tag="S1361_RS21020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20960"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_208033352.1"
                     /transl_table=11
                     /translation="MPAENAPAAGNLDDDDYPAYTMGRAAAVLGTTPAFLRAVGEAGLI
                     TPLRSEGGHRRYSRRQLRVAARARELVDQGTPVEAACRIVSLEDQLDDALRKNRDMRRK
                     LGERGADT"
     gene            complement(8818..9471)
                     /locus_tag="S1361_RS21025"
                     /old_locus_tag="S1361_20965"
     CDS             complement(8818..9471)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959840.1"
                     /locus_tag="S1361_RS21025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_20965"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_208033353.1"
                     /transl_table=11
                     /translation="MRIGELSRRTGVPVPTIKYYVREGLLPAGELTSRNQASYDETHER
                     RLRLIRALLDVGGMKVAEIADVLAVVDDPQRPLHKVLGAAAGRVGGGDAEEEDAESAAA
                     RDVVDRLISRRGWRTNTANPAAADLARALAAMARLGHGAFADLLDAYADAAEEVARADL
                     AYVDRQLAVEDMLEGVVIGTVLGEAVFSALRRLAHVDASAKLYGDSGPHERGTS"
ORIGIN
        1 ccaactggga caccctcgac caccgcgggt acagcgggca gccggtccag ctccagttcc
       61 gcaaggccgg tacgtcgacg tacaccaccg tcaagacggt gaccacctcc agcaccggca
      121 ccctgtcgac cacggtcccc gcggccgagg acggctactg gcgctggagc ttcgcgggca
      181 cctccaccac cccggcggtc cgggcggccg gcgacttcgt ggacgtgcag taggcgctcg
      241 gcggcaggcg gccgcccgca gcgccgcacc gcacccgcgg agccggcccg tcccggctcc
      301 gcggtcgcgt ccggcccggc ccgtgtccgg gtggttccgg gggtgtggct gagggatgtc
      361 tgagggtctc ggggtggaac ccctaggggg cggcccgtag ctccccctgg agtgggcccc
      421 ttcgcgttgt cccgcgtcag cggacgtgca agtctccgta agcaggtgat gacgcggcga
      481 ggcacgcgac aaccgaggga agttggtccg ggtggacggt acgcactgga tgtacgtgct
      541 gctcgtgctc gtgacgatgc cgcccatgat cccgaactcg gctctggtgg ccggcgccgg
      601 cgccctcgcc gcggcgggca gcctgaacct gcccctgctg atcgtgatct tattggcgag
      661 caccgtcctg ggcgacatga gcatgttctg ggcggggcac ctcgcccgca gccgtgcgct
      721 gcgctggatg tcccgccggc agaagcggcg ttcgatgctg gactgggccg ccgagcggtt
      781 ccgccgctac ggcgtgccgt cggtgatcgt gatgcgcttc gtgcccagcg gccgcggcct
      841 cggcggggtc acagccggga tcgtggactt cccgctgcgc agctatctcc tcggcgccct
      901 gctcgccgag gccgtcttcg tgtcctgcac cctgggcctc gggtatctgg gcggacggct
      961 cgtcgacaac agcgtgacgg tggtgtgcat ggggcccgcc gtctccgtcc tcaccgccgg
     1021 cagcgtcatg gccgtccgct gggcctggac gcggtggagc ccccgtcgtg ccggtgaccg
     1081 cggtgactga cctgacgcgg ccgacagccg acacgatcga tcccgactcg accggtcccg
     1141 actcgaccga gcccggtacg accgtggacg tcatgcccgc cgaccgactc gcccgtgtgc
     1201 tcggaaccct ggcgaacccg caccgcctgg gcgtcgtggc cgccctcgcc cggcgccgca
     1261 actacgtcag ccggctggcc cgcgaactcg gcatcagcag agccctgctc caggcccacc
     1321 tcagacggct ggaagcggcc gggctggtga cgggccaact ggagatctcc agcgacggga
     1381 aagcgatgaa gttctacgag gtgaccccct tcgcgctcca tctgacaccg cagagcatcg
     1441 cggacgcgct gtcacccctc accatccacg cacacgtccc ggaggacggc gagaagggaa
     1501 gcccgcaatg agttaccacg gctggcagga ggtcgtcggc accgtcggtt tggtcggtgc
     1561 gttcgcatcg gtcgtcacca tcgtcatctg gcacatggcc atgacctggc gggcccgggc
     1621 cgtcctggcc cgcgaccacc agtaccggac cctcgcggag caggccaccc ggtcacacga
     1681 ctccgcggta cagcaactcg ccggcatcga cgagcgcctg gccgatctgc agacccggat
     1741 gcggtcggtc gagcggatcc tcaaggaagt cgagtgacgg cgtaaaagga cccgggccgt
     1801 cgagcggcaa ctcggcggcc cggtggaaga ggcactgtcc cgcgtcgaac gcgagtctct
     1861 caccagcccc ccttcacgga aggaccctaa ttcatgccgc ccaagggcaa gcggttacag
     1921 ctgcagaacg gcacactgat cgaacgcgtc gccggctggt cgatccggca ccgcgcactg
     1981 gccatcggag gatggctggc actcgtggtg ctggccgtcc tgtccacgtc cctggtgtcc
     2041 ggcgacagcg ccgtcaccaa ggaccccggc gaatccggtg cggcccagca ggtcctgaac
     2101 gaacagaagg actgggaacc cgtcctggag aacgtactcg tccagtcgcg cgacaaggac
     2161 gggccggcat tcaaggacga cccggaactg cagcaggcgg tcaaggacct ggtcgccgaa
     2221 ttcggaaaga cgcccggtgc ggtcttccga ctgcgcaccc cgctggatcc gcagggcgaa
     2281 cagcagatct cggcggacgg acggtccggt ctcgtcacct ttttcgtcgc cgggcccaac
     2341 gtgaaaatgg acgcccattt cgacgcggtg gtcgacgccg tgcacaaggt ggccgaccgg
     2401 cacaaggaca tccgggtcgt acaggccggt gaccagagcc tttcccgggc cgtcgacgac
     2461 gcggtgaaga aggacttcgc cggcgccgag agcacctccc tgccgatcac cctcgtcatc
     2521 ctgctcatcg tgttcggcgc gctggtcgcg gcggccgttc cgctgctgct cgccgccacc
     2581 gccgtggccg ccgccttcgg actgatcggc gtgttcggcc agtgggtggc cgtcaacagc
     2641 gccgtctcct cgatgatcct gctgatcggg gtcgccgtca gcatcgacta ctcgctgttc
     2701 tacctgcgcc gggaacggga ggagcgggcc gccggccgca ccgtcgccga ggcgctgcgc
     2761 atcaccgcca agacctccgg gcacgtggtc gccgtctccg gcgtcaccgt catgctctgc
     2821 gtcagcggtc tgctgctgag cggcctcgac gtcttccgcg ggctcaccct cggcacgatc
     2881 ctcatcgtcg gcctggcgat gaccggctcg gtcaccgtcc tgcccgccct gctcgccgcg
     2941 ctcggccacc gggtggacaa gggccgcatc ccctggctcg gcaagcggcg caccaccgtg
     3001 ctggagtcgc ggacctggcg ccggctggcc cagaaggtcg tcaagcggcc cctggtgtgg
     3061 ggcggtgccg ccgtcgtggc cctcgtggtg atggctctgc cgatgttctc catgcacctg
     3121 caggacgccg ccgtcgtcaa cagcctgccg cgcagcgcgc cgaccgtgga cgcggcgatc
     3181 cggatgcagg acgccttccc gggatcgccc accccggccc ggatcgcggt gtggaacgcc
     3241 gacggcggct cgccggacac gcccgcggtg cgcgccgccg tcggcaggct gcacgacgag
     3301 atcgccgcga gcgacggccg gctcttcggc cccgtctcca cggtccgcgt cgacgacgtc
     3361 ctcgtggtcc gggtgccact ggcgggctcc ggcaccgacg acgactccca ccgggcgctg
     3421 gagaccctgc gcgacaccgc cctgccccgc acgctcggca aggtggacgg cgtcgagtac
     3481 gccaccgccg gacggacggc gttcgcccac gacttcgccg gccggctgag cggccgcacc
     3541 ttcgccgtca tcgcgttcgt gctcgtgctg gcgttcgtcc tgctgctcct ggtcttccgc
     3601 tccctgacca tcccgctggt ctccatcgcc ctcaacctgc tgtcgatggg cgcctcgtac
     3661 ggcgtgctga cctgggtctt ccagtacggg aacctcagct cgccgctcgg cttcacctcg
     3721 tacggcgggg tcgcggaatg gctgccgctg ttcatgttcg tggtgctgtt cgggctcagc
     3781 atggactacc acatcttcgt cctgagccgg atccgggagc gccggcaggc cggcgccgag
     3841 ccacgggacg cggtggtcgg cggcgccgcc gccagcgccg gcgtggtcac gagcgcggcg
     3901 gtcatcatga ccgcggtgtt cacgatcttc atcaccctgt ccgcgatcga gaacaagatg
     3961 atgggcgtcg gcatggccgt ggccatcctc atcgacgcca ccctggtgcg cggcatcctg
     4021 ctgccggcgg ccctggccct gctgggccgg cgcgcctggg aactgccgcg cggtctgcgc
     4081 cgcctgccgg gcggcacgtc cgggtccgcg cccgccggcc ccaccggcgc cgtgagccgc
     4141 ccagccgagg gcgtacgacg cagctgaacc agcccccgtc ccccgctccg gccgcaaccc
     4201 gatactcccg ggttccggcc ggagccgcgc cgatccagac ggagtaccgg gccctgcaga
     4261 gtgctgtcgc gcatgacccc ggtactcgat ctggtgggcg gaccccgaac ggcccgccgg
     4321 atggcgtgcc gagggaaagc agggaaagcg ctcggagcag gaagccacgc actgacgcac
     4381 acggcagccc agtccggcac gaccaccgat tccggccgga ccgggatcgg tccttcggcg
     4441 agcggcagca gctggtggtc ctggacgcgc tggagtccga gcgggtcgag gcgctgcccg
     4501 gggccggcgt gaccgagttc cgcggcggcc acttctacgt ggaggagcgg ctcaccgagg
     4561 tggccgccct cgtcgacgcc accctcggct gcacaccggc tgagccgcgc cggcgccggc
     4621 tgcccgccgg tttcggtgaa tctgcacagc gcgtgcgcaa tccccgctcg tgcgccggga
     4681 atcggcgcgc gggcccgccg caggattccc cgttcgcgcg gagtgtcccg ttccgccgac
     4741 ggggacgtcc gcggcgccgg gcgagggagc cgtgggaaac ggcgacggag ccgtggggaa
     4801 tatggagagg ctgtcctggt gccgtgtgcg ggcctgcacg gatgcgaccg cgaaacgccc
     4861 ggcttccccc gcgggcgcgc cggtcggctg gatgacgggc gtggtccgtg ctttgatccc
     4921 cagcgccgcg agacccgcga tccgtcccca gcggggccgc cccccccttg tggctcatcc
     4981 atacgaaggg aagtctcctc atgaactccg ctccccaggt cgagaccgcc gagatctccg
     5041 acgccgagct ggacaacgtc tccggcggcc tgagcccgca cgccggcctg accgccggcc
     5101 ccacgacggt cggcgacgcg gacgtcctgg ccgcgctcgg caccgtccag aaccaggtcc
     5161 tggccaccgc cgcccagtac gaccaggtca gcgtcagcgt ctccctctga cgcacaagac
     5221 ggagcgggct gcaccccccg gggtgcagcc cgctccgcgt gcgtcaccag ccgagcgcct
     5281 cctcgtcggc ccgtgtctcc agggccggga cgaccgggcg cggctcgtgg caggtgaagc
     5341 cgaggcgggt cagggcccgg cgcatctcgg ccaccgtgaa gtcgcggcgg ttctgccggg
     5401 tgatcacttc accgacctgc atcacggggt agacgcggcg gccgatcgtc acggacacgc
     5461 cggtgacgga ttccggcgtg atgccgctca tggcctgttc gacctcgctc ttgaccaggt
     5521 cgaaggggaa acgggcgatg acacagcgca tggatgcctc cacgaggtgg ggaagacggc
     5581 ggtcagggcg tgagggccag gacgcccagg gcgtatccgt gctcgtcgat cacgggggac
     5641 acgtccaggg tccgcacccg catggcggct tccgcctcgc cgagcgcggc ccgggacgag
     5701 gtgaacggtc cgcggtcgag cgggaggtcc cgcagccgcg tccggtcgct gtaccacgag
     5761 ccgccgcggt gcgcggtgag ctgggcccgg gtgaccaggc ccgcgcatcg cccgtcctcg
     5821 tcccgcagga gcaggtgcgc gacccgcgcg ccgatgagca cggacagggc cacgtcgacc
     5881 gccatgtggt cgtcgacctg cggcccgggg gcgtgcatgg cgtcgcgggc ggtcagaccc
     5941 gtgccggcgg ggctgccggc ttgctgggtc cgggacggaa acggggtcaa gggcttctcc
     6001 tcttcgtcgg ggcggaccgg acgggccacg ggcccgtccc gtccgccggg agtgcgtccc
     6061 cgtgctcggg ctatgcgttg ttccgcggag cgggccgacg gccacgggcc tccccgccgc
     6121 ggccggcgcc ctggccgccc tccccgcggc cggtgcccgc gctcctgcca cggcccgcgc
     6181 cgcccctgcg gcgccgaccg gcggacgatc ccttgccctc acggcggtcc tcggccggcg
     6241 cggtgaggac gacgggcacg ccggaaggcg tccgggcgcc ggtgatccgc tgcagctcgg
     6301 gctcgcccgg gcggacccgg gcggcgttcg ccgcgatgcc ggcgtccgtc atgagacggt
     6361 ccaccgcgcg ccgttggtgc ggcaggacga gggtgacgac ggtgccggac tcgccggcgc
     6421 gcgcggtacg gccgccgcgg tggaggtagt ccttgtggtc gccgggcggg tcgacgttca
     6481 cgaccaggtc gaggccgtcg acgtggatgc cgcgggcggc gacgttggtg gccaccagca
     6541 ccgtgacgtg accgtccttg aactgggcga gcgtccggtt gcgctgggac tgggacttgc
     6601 cgccgtgcag ggccgaggcg cgcaccccga cggagagcag gtgcttggtg agccggtcca
     6661 ccgagtgctt ggtgtccagg aacatgatca cccggccgtc gcgcgcggcg atctcggtcg
     6721 cggcagtctg cttgtcgtcg tcgcgcacgt gcagcaggtg gtggtccatg gtggtgacgg
     6781 cgcccgcgga cgggtcgacc gagtgcacca cggggtcgtg gaggtagcgg cgtaccagga
     6841 ggtcgatgtt gcggtccagg gtggcggaga acagcaaccg ctggccgccg gcgggcacct
     6901 ggtcgagcag cgcggtcacc tgcggcatga agcccatgtc ggccatctgg tcggcttcgt
     6961 ccaggacggt gacgcagacc tcgtcgagcc ggcagtcccg ccgctcgatg aggtccttca
     7021 ggcgcccggg agtcgcgacg acgacctcgg cgccgccgcg cagggcgctg gcctgccggc
     7081 cgatcgacat gccgccgacc acggtggcga gccgtagccg cagggcgcgg gcgtaggggg
     7141 tgagcgcgtc ggtcacctgc tgggccagtt cccgggtggg gacgaggacc agggcgagcg
     7201 gctgccgggg ctgggcccgc tgtccgtcca gccgggccag cagcggcagg ccgaaggcga
     7261 gggtcttgcc cgagccggta cggccccggc ccaggacgtc ccggccggcc agcgcgttcg
     7321 gcagggtggc cgcctggatc gggaagggca cggtgacgcc ctctgcgccg agcgcggcca
     7381 acagccgtgc gggcatgtcc agttcggcga acgactcgac cgccggcagc ggttcggtga
     7441 aggtggtggg cagggcgaac tcctgccggc gcggggtgga gtgcgcggcc gggccgttct
     7501 tgcgtccggt gcctgtgcgg gggccctgac ggcccggctc ctgggtacgg aagcggccgc
     7561 cgtgcgcggc tcccgccgag cgggcggacc tccggtccgc gcggaagcca ctggacgcgc
     7621 cgccggagcg acggggggtg cggttcatga gggaaccttc ctcgatgcgg cgtatcgagg
     7681 aattccgtcc agcggtgagg accgcacgag gattcgcaag aatgagccga agaaaatgtg
     7741 aagagccgga cctcgcggcg tgacagccgg aggtgtccgt ggtccgggaa atccggaaaa
     7801 ggggctcaga caatgagcgg gggcccgcac cccaagagcg cgggccccag ccacatgatg
     7861 cgcgaaccac cggttcgcga cgatttcgcg tcagcgtcag gcgggaacga tgttctcggc
     7921 ctgcgggccc ttctggccct gcgtgacgtc gaaggtcacc ttctggcctt cctgaagctc
     7981 gcggaagccg gaggtggcga tgttcgagta gtgcgcgaac acgtcggcgc cgccgccgtc
     8041 ctgctcgatg aagccgaagc ccttttccgc gttgaaccac ttcacggtgc cagatgccat
     8101 attgaatctc cctcggaggg cagtgccgga ctccgcactt tacggagccg agtcgccgcg
     8161 atgatcaccc ctccggaagg atccggaaag ctctgcaaag aaaaaaatct ctggcaacca
     8221 aaactgcaac tgctatcacg ctaacacagg tcggttggcg acgcccggct gttttgcgtg
     8281 gcgtccggca attctcgtgc cgccgcggac cgtaattctg tatcggcggg actaggtatc
     8341 cgcgccgcgc tcgcccagct tccggcgcat gtcccggttc ttgcggaggg cgtcgtccag
     8401 ctggtcctcc agcgagacga tgcggcacgc ggcctcgacg ggcgtgccct ggtcgaccag
     8461 ttcgcgggcg cgggcggcca cccgcagctg ccggcgggag taccggcggt ggccgccctc
     8521 cgagcgcagg ggagtgatca gccccgcctc gccgacggcc cgcaggaagg cgggggtggt
     8581 gccgagaacg gccgcggccc ggcccatggt gtaggcgggg tagtcgtcgt cgtcgaggtt
     8641 gccggcggca ggggcgttct cggcgggcat ggcaccttcg tttcggggga tgcggcggga
     8701 tgcccgggcg tccggggcac ccgcgctcgg cagactgggc gccatctgcc tcctgccgca
     8761 accctaacgc cgccgggcgt gaagacctgg tcccgccgtg acagatttcc gcgcggttca
     8821 gctcgtcccg cgctcgtgcg gcccgctgtc gccgtagagc ttcgcagagg cgtccacatg
     8881 ggccagccgc cggagcgcgc tgaacaccgc ctcgcccagc acggtcccga tcaccacgcc
     8941 ctccagcatg tcctcgaccg ccagctgccg gtccacgtac gcgaggtcgg cgcgggccac
     9001 ctcctcggct gcgtcggcgt acgcgtccag caggtcggcg aacgccccgt ggcccagccg
     9061 ggccatggcg gccagggccc tggccaggtc ggcggcggcg gggttcgccg tgttcgtccg
     9121 ccagccgcgc cgggagatca ggcggtcgac gacgtcccgg gcggccgccg actcggcgtc
     9181 ctcctcctcg gcgtccccgc cgccgacccg gcccgccgcc gcgccgagca ccttgtgcag
     9241 cgggcgctgg gggtcgtcga ccaccgcgag cacgtccgcg atctcggcca ccttcatgcc
     9301 ccccacgtcg agcagggcac ggatcagccg cagccggcgc tcgtgcgtct cgtcgtagct
     9361 cgcctggtta cggctcgtca gctcgcccgc gggcagcagc ccctcccgga cgtagtactt
     9421 gatcgtcggc accggcacac cggtccgccg gctcaactcc ccgatacgca ctgccggttc
     9481 actcctcggc ctccgccctg cccttgccgg caggacctcc atgatagata gtggggctat
     9541 cggatagcgg atagtgccac tatccactct gctggtcctc tcactcacgg gggtagtcat
     9601 gcctgcctta ctcacgtcct cgcgccccgc gccgtcgggg tcgcccgcgc agtcgtggcg
     9661 gtcctggcac cgcccgctgg tgctgttctc ggcggtcatg gcggtcacgg cggtgctgtg
     9721 cgcggtggga ctcgtggtgg acgaccgcgt cctggtgggc gcgccggtct gggccaagcc
     9781 gctgaagttc tcggtctcct tcgtggcgta cagcctctcc ctcgcctgga tgctgtccct
     9841 gctcccccgc ggccggcgcc tggcctggtg ggcggggacc gtcgtcgcac tggcgagcct
     9901 ggtcgagatg ctgatcatca cggggcaggc cgcacgcggc aggcgcagcc acttcaacca
     9961 ggcgaccccc ttcgactccg ccctgttcca ggcgatggcc gtcacggtcg tcatcctgtg
    10021 ggccgccacg ctcgtcatcg ccgtgctgct gctgcgcacc cggatcgccg accgcgcctc
    10081 cgcctgggcg atccgctccg gcatcgtcct ggccctggcc ggcgccgccg tcggcttcct
    10141 gatgacccgg cccgccccgg ggcagcggcg cggggtctcc aaggtgatcg gcgcgcacag
    10201 cgtgggcgtg
//
