LOCUS       NZ_CP071839            10495 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3318899
            Orig. end                         :: 3329394
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10495
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="12"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10495
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10495
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5495](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5495)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    604..617
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    1118..1136
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     misc_feature    complement(1129..1145)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1134..1148)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     tRNA            1392..1467
                     /anticodon=(pos:3320324..3320326,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="S1361_RS14840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="S1361_14825"
                     /product="tRNA-Arg"
     gene            1392..1467
                     /locus_tag="S1361_RS14840"
                     /old_locus_tag="S1361_14825"
     gene            1885..2169
                     /locus_tag="S1361_RS14845"
                     /old_locus_tag="S1361_14830"
     CDS             1885..2169
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056511.1"
                     /locus_tag="S1361_RS14845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14830"
                     /product="hypothetical protein"
                     /protein_id="WP_208032324.1"
                     /transl_table=11
                     /translation="MIVYPLWHVGHQNEAGDDGSTVHVDESGVFCDESDGDDVKLLGIY
                     STLERVEERVRQARLLPGFRDEPECFYFDACELDEDDWTEGFVRVPPGE"
     gene            2331..2498
                     /locus_tag="S1361_RS14850"
                     /old_locus_tag="S1361_14835"
     CDS             2331..2498
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="S1361_RS14850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="S1361_14835"
                     /product="hypothetical protein"
                     /protein_id="WP_208032325.1"
                     /transl_table=11
                     /translation="MEDLTASVVTRVLRTVGSAASFVVDMLGVRDWIKSVRRDKREKAG
                     PVTTPPDIQS"
     gene            complement(3028..3243)
                     /locus_tag="S1361_RS14855"
     CDS             complement(3028..3243)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604684.1"
                     /locus_tag="S1361_RS14855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_243769175.1"
                     /transl_table=11
                     /translation="MSQGLRRISGLTVLVAGIALGAWVVFGAPHHWHGAKALGRLALGL
                     GAMAMVSGSPRLIFPDTPQGGHRTGT"
     misc_feature    complement(3229..3231)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3252..3265
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.13"
                     /tool="antismash"
     gene            3407..3628
                     /locus_tag="S1361_RS14860"
                     /old_locus_tag="S1361_14840"
     CDS             3407..3628
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527018.1"
                     /locus_tag="S1361_RS14860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14840"
                     /product="hypothetical protein"
                     /protein_id="WP_208032326.1"
                     /transl_table=11
                     /translation="MTKPAAPKRHLPTSPFKAPVPPVPKHFAMGDQVTHDMYGLGRVIG
                     IEDGVAALVDFGSTQVRVLSPYAKMTKL"
     gene            complement(4151..5017)
                     /locus_tag="S1361_RS14865"
                     /old_locus_tag="S1361_14845"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="S1361_RS14865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14845"
                     /product="tyrosinase family protein"
                     /protein_id="WP_208032327.1"
                     /transl_table=11
                     /translation="MVYVRKDVSTLTASERRRFVNALLELKRRGEYDEFVRTHMDYYAP
                     DGEAGLRTAHMAPSFLPWHRRFLLELERALRRVDSSVTVPYWDWTRDRKKTSVPWTKDL
                     LGGNGRSSDRQVMTGPFAYSAGHWTIKENTTDANFLTRDLGRSAAPIELPTAGDVKKAL
                     DDPVYDASPWDSTVTKGFRNKLEGWGTGSGSASWRNHNRVHRWVGGAMLGSASVNDPVF
                     WLHHAFVDLLWDRWQRAHRNHRYLPADPPGATDKQHRRVVARHERLPPWDETPDQLENL
                     SRIYRYG"
     gene            complement(5001..5495)
                     /locus_tag="S1361_RS14870"
                     /old_locus_tag="S1361_14850"
     CDS             complement(5001..5495)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="S1361_RS14870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14850"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_208036602.1"
                     /sec_met_domain="melC (E-value: 9.1e-44, bitscore: 147.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGADGVAVGAERRTTAGAGRPSRREVARGLLACAAVLAPAPVV
                     TASRPALPARHPDRGCFEETYRGRRIQGVAVPDAGHETTDCDWRITVDGSPLHLMRRAD
                     GTWLSMVDHYTSYRTPLEATRAAVDELAPGLRLRGPVPGSPDGQPAGGHAHGGGHHGVR
                     A"
     gene            complement(5804..6094)
                     /locus_tag="S1361_RS14875"
                     /old_locus_tag="S1361_14855"
     CDS             complement(5804..6094)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136064.1"
                     /locus_tag="S1361_RS14875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14855"
                     /product="hypothetical protein"
                     /protein_id="WP_208032328.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLALAGVAAPAFAASGADLSPQNVSGTLDKLTG
                     GPVTTQKVLPVQGQSVSLDTEKKDSPLGTVKGAASSLTQRGGLLGGLPVHH"
     gene            complement(6209..6934)
                     /locus_tag="S1361_RS14880"
                     /old_locus_tag="S1361_14860"
     CDS             complement(6209..6934)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="S1361_RS14880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="S1361_14860"
                     /product="hypothetical protein"
                     /protein_id="WP_208032329.1"
                     /transl_table=11
                     /translation="MRVLPTRRLPSAALCAAVLVAVTGPAALAAHHVQEYEHTASRPAV
                     PGAENLLAQAGSLGAAHPVLHPVVELLDQALHKGTLPADQAKRLGDAAKKAVAEAVAHP
                     AALAVPAAPALPATPAASGTPTATATSSALAHPSMEPSFSMLVPPHPEAAGDDGNQLAV
                     RDLSGDRQALVQAIGNLVNAVGTDPGQVLPAATGVLTGLVGLITGILGAALPTASVSAS
                     VSASVSASASVSAPSPATG"
     misc_feature    7104..7118
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.27"
                     /tool="antismash"
     gene            7167..8246
                     /locus_tag="S1361_RS14885"
                     /old_locus_tag="S1361_14865"
     CDS             7167..8246
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="S1361_RS14885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14865"
                     /product="DUF3344 domain-containing protein"
                     /protein_id="WP_208032330.1"
                     /transl_table=11
                     /translation="MRKFLRPLLRTAAGAVALAALPATAVARPVAPPSPETPRLAFERR
                     YHAVQHGGFVRAANATISCRAPEAPSCTAVRDGRTAVNGDFDMFYVDVDRDPNTYNSSR
                     AEVRVPAGGRVTYARLYWGGNLRVGEQKPPQDNGRVLIAEPGGEYKELLADTVVGHRVA
                     QGADAFQASADVTELVRKSGSGLYTVAQVNTAMGRSAAGAWGGWTLVVAYENAAEPLRD
                     LSLWDGFDSLDSPVDVQLSGRRVPAGAGGRVGLVAYDGDRGIVGDSLTLSTGHRSKPVA
                     LGDAADPADDVLNSTISEPGDVPARRVPAYANTLGYDSDVFDLGSSLRGGGDQLGFRLV
                     SHRDAAWAGALFAAVDARN"
     gene            8301..9572
                     /locus_tag="S1361_RS14890"
                     /old_locus_tag="S1361_14870"
     CDS             8301..9572
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065680.1"
                     /locus_tag="S1361_RS14890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_14870"
                     /product="glycosyltransferase"
                     /protein_id="WP_208032331.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.5e-32,
                     bitscore: 110.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQPAPGSRPRVLHLAQPVDGGVARVVRDLVQAQLAAGLHVTAAC
                     PGGQLAEELRDLGTDVRDWQARRSPGPGLPGEVRRLVHLIGEVRPDLVHAHSAKAGLAG
                     RLAVRGRVPTVFQPHAWSFEAVGGLTAAVALRWERWGARWADRIVCVSEAERTSGLRAG
                     VRARYTVIPNGIDVDRFAPAPSTGAAPGSAPVTAHAPQTAGGGIAPPEPLAPGHEGRAG
                     RAGGSSQDESGPPPVVVCVGRLCRQKGQDVLLRAWPDVLEAVPDARLVLVGDGPDRDAL
                     RRRAHPSVRFTGAVADPVPWYRAADLVVLPSRWEGMALAPLEALGCGRPVVLTDVDGAR
                     ESLPAALAARCLVPPEDPAALAGAIARLLSDPPLRATLGDLGRRHVLSAHDVRHTAEQV
                     AVLYRELLGDRPPVPSECREPIHS"
ORIGIN
        1 ctctccacga gggcgtccag gagttccccg gcggtgcagt ccagggggaa ggcgcgccag
       61 cgcttgaggg cgtccccggc gagtacggcg ccggactgga ggcgggcccg cacgcgcgcg
      121 tgctccttgt cgtaggcggc ctccacggcg gaggtgagcc ggagggccgc cgcgtactgg
      181 gcggcggcgg cgctggccag ctcgggcatg cgcgccttga gcgagtcgag gacgccgtag
      241 gccgtacggg ccatgacgtg ctgccgggcg gcggggtcct gggcgtgatg gacgagccag
      301 ctcttcaggg gcgcgacggc ggtggcgggc agcaggccgg cgccccaagc ggactcgggc
      361 agctcgggca cggtgaagcg gggtacgtcg ccgagtccgg ccttggtgag cagggcgccg
      421 tactgccggg agacctcgga gacgacctgg tggggaaccc ggtccaggac ggtgacgagg
      481 gtcgcgtcgt actccttggc ggtgcgcagc aggtgccagg ggacggcgtc ggcgtagcgg
      541 gcggccgtgg tgaccatgat ccagatgtcg gcggcgcaga tcagttccgc ggcgaggacg
      601 cggttgtccg cgatcagtga gtcgatgtcg ggggcgtcaa ggagggcgag cccgcgcggg
      661 agggtgtcgg cggtctcgac gcgcaccacg cgcgcggggt tctcgtcgga gatcatgaac
      721 tcgtcggcgg ggtcctggtg gggcacccac acgcgtgtga gatgggggag cacgcgcatg
      781 ccgctgaacc agtgatgatc ctccggatgg cagaccagca ccggcgtccg ggtggtcggc
      841 cgcagcacac cggcctcgct cacgcgccgg ccgacgaggg agttgacgag cgtcgacttg
      901 cccgcgcccg tggagccgcc gacgacggcc agcagcggcg cctcggggtc ccgtagccgc
      961 ggtactaggt agtcgtcgag ctgtgcgagg agttcgtcgc ggttggcacg cgcgcgtggc
     1021 gcccccacga ggggcagcgg aaagcgtgcg gcggcgacac ggtcgcgcag agcggagagt
     1081 gtgtcgagca gctgaggccg tacgtccaag gtcaccacat gcgaagaatg cccaatttta
     1141 ggggaattct gaagcatata gacatgtctg cgcgccgaca gaacagaacg gacggaaggg
     1201 acgaccggga catgggcaca ggccaggcat aacgagtaca caacacccgg tgcgtcggac
     1261 gccaaaagcg gtgcacgatt cgtacctgcc tgcgattatc aggaccgctt caccgaacct
     1321 ccacatcgag ccacggaggc gaagcgacag ggtcgaggaa cagggagctt tatccttggt
     1381 tccgccacca cgcccccgta gctcagtgga tagagcaggc gccttctaag cgcttggccg
     1441 caggttcgag tcctgccggg ggcgccacct cctaccagca aaaacgcccc ggaggggatc
     1501 tttcctccag ggcgtttcgt gtctctgacg gcaaggctga cggcaacggc cccgtcactc
     1561 gtccagcagg ctgcccagcc gatcaaggct cgcttcgctc gctggtccgg cggccaacgg
     1621 cgctcttgga tcatcgtcaa cccgaccgcg gcaccctccg cccaagcgca ccaacggccg
     1681 ctcccggccc caccccaccg ccaccaactt ccggtgggtc ggatcaagac ccgagggccg
     1741 cgacgcctcg cctgtcttcg ccaccggccg cacgtcgccg cccgtccgtc gccgcacgca
     1801 gcgggcgcat tccaacacgg gcacgggggc gaacggtgac gggcgccgaa cgtccacgaa
     1861 ctcaagtgct ggtagcttcc gcccgtgatc gtgtatccgc tgtggcatgt cggccaccag
     1921 aacgaagctg gcgacgacgg ctcgacagtg cacgtcgacg agagcggcgt cttctgcgac
     1981 gagtcggacg gcgacgacgt aaaactgctc ggtatctact caacccttga acgggttgaa
     2041 gagcgcgtcc gtcaggctcg actgctaccg ggcttccgtg atgaacccga atgcttctac
     2101 ttcgacgcct gcgagctcga tgaggacgat tggaccgagg ggttcgtccg agttccgccg
     2161 ggcgagtagg cccgtggaca gggtctcggt gggatgccgt tccgtggtca ggtaagcccc
     2221 aggccccaga gaagctgccc gaggacactc aacgccgacc gatgtgacag acgcaccacc
     2281 gccgccagca gtgaaccccc ggtcggagcc gcaaccagcc aggagcgcac atggaagacc
     2341 tgacagcatc ggtggtgacc cgcgtgctga gaactgtcgg ctcggcggct tccttcgtcg
     2401 tggacatgct cggcgtccgt gactggatca agagcgtcag gcgcgacaag cgggagaagg
     2461 ctggtcctgt gaccacacct cctgacatcc agagctgaca tcaacgacgg cagacgcgag
     2521 cggatgccga aggctccgga cggattggac atggccacga cgccatggca gtccagggcg
     2581 gcgaccgccc tcccgtccaa ctcctgatgc ggtggtcgca cggtggcggc gccgacggga
     2641 gtgacggcag tgctgacggc tacgacggcg gacggcagtg gttttcggcg gcccggcacg
     2701 atgccggcat aggacgatcg gagctcctca ctagggcgtg cgcgcatctt cccagcgctt
     2761 ggccgcaggt tcgagtcctg ccggggcgcc caccctcagc cctcccggag ggagggcttt
     2821 tgcgtcagag cggatggcgt cggccacgac gcatccccgc ccgcccgctt ggtgatcagg
     2881 ggccggctcc ggggccgtcc ggctgccccg cgtcttcgca cgccggccgg ttcgcatgcg
     2941 tgccggagct ctcggcccgg ggaagatcag ccaacgcccg gcgccccggt atccgtgggc
     3001 gtacggacca gccttcgcac gggtgcgtca ggttccggtg cgatgaccgc cctgcggagt
     3061 gtcgggaaag atcagccgcg gtgaaccgct gaccatcgcc atagctccca gcccgagtgc
     3121 cagacgcccg agagccttgg ccccgtgcca atggtgcgga gccccgaaga cgacccaggc
     3181 acccaacgcg atgcccgcga cgaggaccgt cagtccgctg atacgacgta agccctgact
     3241 catggggctc atcgtcaccg atcaaggatc agcgctcaag ctgcggtccg ttttcggctc
     3301 gtcaggaact cggaaaccgc aggtcagtga cgtgaagcgg tggttctcgt ccggatctgt
     3361 gccgacggcc acagcaaagg ggaggcaact aggctcatac tgagccatga ccaagcctgc
     3421 tgcgccgaag cgtcatctgc ctaccagccc cttcaaggcc ccggttccac cggtccccaa
     3481 gcacttcgcc atgggtgatc aggtcacgca cgacatgtac ggcctcggcc gagtgatcgg
     3541 catcgaggac ggagtcgcgg cgctcgtgga cttcggctcg acgcaagtgc gtgtcctgag
     3601 cccgtacgcc aagatgacca agctgtagca ccgaccgctg tgcccggtca cggcacccca
     3661 ggccccttcg gggacctgca ccgaaagaga gccttctcat cgatctgaca tcgctgttct
     3721 ccgctccgga agggcaaccc cgccgctccg cagcggcgtc gcccgctccg gccatcaacc
     3781 ccttccaggc ccccgatttc ggggaagagg aatcctttcc gaccgaggac gcgcaggcag
     3841 cttgccccag cgaatgacgg tgggacgggc aacgcctgct gcatgactca cctgtggccg
     3901 aagcgaggca ggcctgcgag tggcgcaccc tgccttcggc ggcggatgca ccaaccagcc
     3961 gccggcacta cgacgttggc gaccacgaca gcccacacac gcgccgcgta ccaccgcggc
     4021 gaactcgcgc aaccggcgtt cgccggcgag gagtccgagc agccgtcggg tcgcccgcgc
     4081 cgccggtgga gccgatggga tcgcccggcg cccggcgccc gcgcatcccg cggggcgcct
     4141 cgggcccgtc tcatccgtag cggtagatgc ggctcaggtt ctccagctgg tccggggtct
     4201 cgtcccaggg tgggagccgc tcgtggcggg cgacgacccg ccggtgctgc ttgtcggtgg
     4261 caccgggtgg gtcggcgggc aggtagcggt ggttgcggtg ggcacgctgc cagcggtccc
     4321 acagcaggtc cacgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gacgcgctgc
     4381 cgagcatggc accgcccacc cagcgatgca cccggttgtg gttgcgccag gaggcgctgc
     4441 cgctgccggt tccccagcct tccagcttgt tgcggaaccc cttggtgacg gtcgagtccc
     4501 agggcgacgc gtcgtagacg gggtcgtcca gcgccttctt gacgtcccca gcggtgggca
     4561 gctcgatggg ggcggcggaa cggccgaggt cccgggtgag gaagttggcg tcggtggtgt
     4621 tctccttgat ggtccagtgg cccgccgagt aggcgaacgg tccggtcatc acctgccggt
     4681 cggagcttcg gccgttgccg ccgagcaggt ccttcgtcca ggggacggag gtcttcttac
     4741 ggtcccgggt ccagtcccag tacggcacgg tcacggacga gtccacgcgg cgcagcgccc
     4801 gctccagctc cagcaggaac ctgcggtgcc agggcaggaa ggacggcgcc atgtgggcgg
     4861 tgcgcagacc ggcctcgccg tcgggggcgt agtagtccat gtgggtgcgc acgaactcgt
     4921 cgtactcacc gcggcgtttg agttccagaa gcgcgttgac gaaccgtcgc cgctcgctcg
     4981 cggtgagcgt gctgacgtcc ttacgcacgt acaccatggt gccctccccc gtgtgcgtgt
     5041 ccgcccgccg gctgcccgtc gggcgagccc gggaccgggc cgcgcagccg caggccgggg
     5101 gcgagttcgt cgacggccgc gcgggtggcc tccagcggcg tccggtacga cgtgtagtgg
     5161 tcgaccatgc tcagccaggt gccgtcggcc cgccgcatca ggtgcagcgg gctgccgtcg
     5221 acggtgatcc gccagtcgca gtcggtcgtc tcgtggcccg cgtccggcac cgcgacgccc
     5281 tggatgcggc ggccgcggta ggtctcctcg aagcaaccgc ggtcgggatg ccgtgccggg
     5341 agcgcggggc gcgaggcggt gacgaccggt gccggtgcca ggaccgcggc gcaggccagc
     5401 agcccccggg ccacttctct gcgcgacggt ctgcccgcac ccgccgtggt ccgccgctcc
     5461 gcacccaccg ccaccccgtc ggcaccgacg accatcgcgc gctcctcgtc cgggttcacg
     5521 tcctgtccgt accgttaacc gagagacggc gcctggggtc accccccgaa agggtcgtcg
     5581 tccgctgacg cgcggtatgc gagcgccggc gtgcgcacgc gcccggcccg cctcaggggc
     5641 cacacggtca tcgggccaca gggccacaga gccgccgggc cacagaggcg cagggccaca
     5701 gagccacaga gccacaacgg tccccgtcac acgatcgggg ggcgccccgg cacgccacgg
     5761 gccggttccg cggatgcggt accggcccat ggggaaggag tcgtcagtgg tgcacgggga
     5821 gaccaccgag caggccaccg cgctgggtga gggaggaggc ggctcccttg acggtgccga
     5881 gcggggagtc cttcttctcg gtgtcgagcg agaccgactg gccctggacc ggcagcacct
     5941 tctgcgtggt gacgggaccg ccggtgagct tgtccagcgt gccgctgacg ttctggggcg
     6001 acaggtcggc gccgctggcg gcgaacgcgg gcgcggcaac gccggcgagg gccagggaac
     6061 cggcgacgac ggcggcggcc ttgagggact tcatcttgtt cctttcttcg gtgactcacg
     6121 tcaccaggtg atgtccgacg ccgtgcctaa cgaggtccgg gcgggccgga aacccccgcc
     6181 cccacaagac atatgagatc ccgaggcgtc acccggtcgc cggggacggg gcggacacgg
     6241 atgccgaggc ggagacggag gcggacacgg aggcggacac cgacgccgtg ggcagggcgg
     6301 cgccgaggat cccggtgatc aggccgacga ggccggtcag cacgccggtg gccgcgggga
     6361 gcacctgacc cgggtcggtg ccgacggcgt tgaccaggtt gccgatcgcc tgaaccaggg
     6421 cctgccggtc gccggagagg tcgcggaccg cgagctggtt gccgtcgtcg ccggccgcct
     6481 cggggtgcgg gggcacgagc atggagaagc tgggctccat ggaggggtgg gcgagcgcgc
     6541 tggaggtggc ggtcgcggtg ggggtgccgg atgccgcggg agtcgcgggc agcgcgggcg
     6601 ccgccggcac cgccagggcg gccgggtgcg ccaccgcctc ggccacggcc ttcttcgccg
     6661 cgtcgccgag ccgcttcgcc tggtcggcgg gcagcgtgcc cttgtgcagt gcctggtcga
     6721 gcagttcgac gaccggatgg aggaccgggt gggcggcgcc gagggatccg gcctgggcca
     6781 gcaggttctc ggctccgggg acggcaggcc gggacgcggt gtgctcgtac tcctggacgt
     6841 ggtgggccgc gagggccgcc gggccggtga cggcgaccag gaccgcggcg cagagggcgg
     6901 cggagggcag acgacgcgtc gggagaacac gcatgggtgt gttcctttcg gtggacagca
     6961 ggacctgagc tcaccctcgg acggcccatc acggtccgca accgctcaca cacggtctgt
     7021 actccaccca gacagcacgg aagcggtcca cccggatgca ggatcacctc cacaacaccg
     7081 acacacttgt ccggtttctc cgtattagtc gggaaatcca ctagagttac ggtcctctcc
     7141 cgcacatgcg aaaggctccg ccagtcatgc gcaagttcct gcgtccgctg ctgcgtacgg
     7201 cggcgggcgc cgtcgctctc gccgcccttc ccgccacggc cgtcgccaga ccggtcgcgc
     7261 cgccctcccc cgagaccccg cgtctggcct tcgaacggcg ctaccacgcg gtccagcacg
     7321 gcggcttcgt ccgcgcggcc aacgccacca tcagctgccg cgcccccgaa gcgccctcct
     7381 gcaccgcggt tcgcgacgga cggaccgcgg tgaacggcga cttcgacatg ttctacgtcg
     7441 acgtcgaccg cgaccccaac acctacaact cctcccgcgc cgaggtccgt gtccccgcgg
     7501 gcggccgggt gacctacgcg cggctgtact ggggcggcaa cctgcgcgtc ggcgagcaga
     7561 aaccgccgca ggacaacggg cgcgtgctga tcgccgagcc cggcggcgag tacaaggaac
     7621 tcctcgccga caccgtcgtg gggcaccggg tggcgcaggg cgcggacgcc ttccaggcct
     7681 cggcggacgt caccgagctg gtccgcaaaa gcggttccgg cctgtacacc gtggcccagg
     7741 tcaacaccgc catggggcgg tcggccgccg gggcgtgggg cggctggaca ctggtggtgg
     7801 cgtacgagaa cgccgcggaa ccgctgcggg acctgtccct ctgggacggc ttcgactcgc
     7861 tcgactcgcc ggtggacgtc cagctgagcg gcagacgcgt gccggccggg gcgggcggac
     7921 gcgtcggcct ggtcgcctac gacggcgacc ggggcatcgt gggcgactcg ctcacgctct
     7981 ccaccgggca ccggagcaag cccgtcgcac tgggcgacgc ggccgatccc gcggacgacg
     8041 tactgaactc cacgatcagc gaaccgggtg acgtaccggc ccggcgggtt ccggcctacg
     8101 ccaacacgct cggctacgac tcggatgtgt tcgatctcgg ttcgtccctg cgcggcggtg
     8161 gtgaccagtt gggcttccgg ctcgtttccc accgggacgc ggcatgggcc ggcgcgctct
     8221 tcgccgccgt ggacgcccgg aactgaggcg cgttccccac cggagtcccg agtgcccccc
     8281 gtgagcgagg tatcccgcga atgcaccagc cagcgcccgg ctctcggcca cgggtcctgc
     8341 acctggccca acccgtcgac ggcggtgtcg cccgggtcgt acgtgacctg gtgcaagccc
     8401 agctcgcggc gggtctgcac gtcacggcgg cctgccccgg cggccaactg gccgaggaac
     8461 tgcgcgacct gggcaccgac gtgcgggact ggcaggcccg ccggtccccg ggaccggggc
     8521 tgccgggcga ggtccggcgg ctcgtacacc tgatcggcga ggtacggccc gatctggtgc
     8581 atgcccacag cgccaaggcg ggcctggcgg gccggctcgc cgtccgcggc cgcgtcccca
     8641 ccgtgttcca gccgcacgcc tggtccttcg aggcggtcgg aggactgacc gccgccgtcg
     8701 cgctgcgctg ggagcggtgg ggggcgcgct gggccgaccg gatcgtgtgc gtcagcgagg
     8761 ccgagcgcac gtccggcctg cgcgccgggg tacgcgcccg ctacaccgtc atccccaacg
     8821 gcatcgacgt ggaccgcttc gcaccggctc cgtccaccgg cgcggccccc ggctcggcac
     8881 cggtcaccgc gcacgcgccg cagaccgccg gcggcggaat cgccccacca gagccactcg
     8941 ctcccggcca cgaaggccgc gcgggtcgtg cgggcgggag cagccaagac gagtccggac
     9001 cacctcccgt cgtggtctgc gtcggccgcc tgtgccggca gaaggggcag gacgtactgc
     9061 tgcgggcctg gccagacgtg ctggaggcgg tgccggacgc gcggctggtg ctggtcggtg
     9121 acggacccga ccgcgatgcc ctgcgccgac gcgcccaccc ttccgtgcgg ttcaccggtg
     9181 cggtcgcgga ccccgtgccc tggtaccggg ccgccgatct ggtggtgctc ccctcgcgct
     9241 gggagggcat ggcgctcgcc ccgctggagg ctctcggctg tgggcggccc gtggtcctca
     9301 ccgatgtcga cggcgcccgc gagagcctgc ccgccgccct cgccgcgcgc tgcctggtcc
     9361 cgccggagga ccccgcggcg ctggccggcg cgatcgcccg gctgctgtcc gaccccccgc
     9421 tgcgcgcgac gctcggcgac ctgggccgcc gacacgtcct gtccgcacac gacgtgcggc
     9481 acaccgccga acaggtcgcg gtcctctacc gcgaactcct cggcgaccga ccacccgtgc
     9541 cctccgagtg cagggagccc atccactcgt gattgcggaa aacaccgtcc cctcccccgg
     9601 cgcgcagcca tgggacaacg gatgcacacc cgtctcggtc ctgccgcccc gcgccgacgg
     9661 cggcttccgg tttccgctgc ggcacccgcc ggaacggctc gcctcgccct tgcccctgct
     9721 cgccgccgac ctgatcgccg cactggtggg cgcggtcgcg ctcgtcccgg cctccggcgg
     9781 cccgctgctg gtggccgtcc tggtgtccgg cgcgctgctg ctgcgaccga actccacccg
     9841 accggtgccg ggggtgctcg aggaagtgcc cctcgtgtgc ggacggatcg cggtgacctg
     9901 gctggggctg gcgaccctgc tggccgccca cgacccggag cgctccctcg ccgcccccac
     9961 cctgctgctc ggctggacac tgcacgcggt ggcggcctgt gccggccggg gcacggtgca
    10021 ctggcggcgc cgggtcgcgc tgctgcggca cccgcgcacg gccctggtga tcgggccccc
    10081 cgcgaccgcg cagcgggtgg ccgcggcgct gctgcggcac ccgggctgcg gggtacggcc
    10141 ggtgggtgtc gtcgccgacc gccccgacgg cagcgcgggc ctgcccgtgc tcaccaccga
    10201 cgaggaggtg gagcgggcgc tcatccagaa cggggtgcgg gtcgtcctca gcgtcgcccc
    10261 cgccgtgcgc accgagaagg gaccgctgct gcgggcgctc gcggagtccg gctgcacggt
    10321 gtgggagctg gacgcggact gccccgcgta cgagacgcgc gaccggctcg ccggattcgc
    10381 cgtccggcgc ctgaaccctg gctcgcggcg gcgcagcgcc ggcaagcggc tgctggacgt
    10441 gacggtgtcc ggggcgctgc tggtactggt cagtccggta ctgctggtgt gcgcg
//
