LOCUS       NZ_CP071839            10405 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2298100
            Orig. end                         :: 2308505
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="9"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    276..290
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
     misc_feature    complement(390..404)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     gene            complement(759..1607)
                     /locus_tag="S1361_RS10235"
                     /old_locus_tag="S1361_10225"
     CDS             complement(759..1607)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="S1361_RS10235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10225"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_208031529.1"
                     /transl_table=11
                     /translation="MKTLAGRRTRLLAATTATAALVLVAAGCSSSDSGSGKTTVKGVHL
                     AKAGQLTTCTHLPYPPFQSEINGKVQGFDVSLIDLVAKDLGVKQQILDTPFENFKTGAF
                     LNSGECDLAAAGMTITAERKKNVDFSDPYFEATQAVLVDKSSGIDSLADVKAKGKKLGA
                     QAQTTGEDYVKSKGYDPISFESSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKANADKFK
                     VVDNLKTGEQYGFTVKKGNKALLDAINKALKDAKADGTYKKIYEQWIGPYNPSAASPAA
                     S"
     misc_feature    1717..1732
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.07"
                     /tool="antismash"
     gene            1743..2843
                     /locus_tag="S1361_RS10240"
                     /old_locus_tag="S1361_10230"
     CDS             1743..2843
                     /NRPS_PKS="Domain: Aminotran_5 (74-310). E-value: 5.6e-27.
                     Score: 86.5. Matches aSDomain:
                     nrpspksdomains_S1361_RS10240_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="S1361_RS10240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10230"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_208031530.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.2e-21, bitscore:
                     73.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLGAERFAAIEDGVARLLDTRQDVLITQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGDWLRDAGATVHDLCVPFHTAVTAEQIREAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLALEACLERIESAGLQTVMDRHRSAALAARAGAVALGGGLEPYVYQEREAAPVA
                     TTLRVPSGVVASDLVARALATDPALPLAAGGGALAKEMIRVNHYGPDATRDAVRDSLAA
                     LGAALTEQGVPVDPAAARRAVEDAWR"
     aSDomain        1965..2672
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_S1361_RS10240_Aminotran_5.1"
                     /evalue="5.60E-27"
                     /label="S1361_RS10240_Aminotran_5.1"
                     /locus_tag="S1361_RS10240"
                     /protein_end="310"
                     /protein_start="74"
                     /score="86.5"
                     /tool="antismash"
                     /translation="RDAGATVHDLCVPFHTAVTAEQIREAFAEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALPHAPAQLEMLALEACLERI
                     ESAGLQTVMDRHRSAALAARAGAVALGGGLEPYVYQEREAAPVATTLRVPSGVVASDLV
                     ARALATDPALPLAA"
     misc_feature    2918..2933
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.1"
                     /tool="antismash"
     misc_feature    2921..2934
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.21"
                     /tool="antismash"
     misc_feature    2927..2941
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.39"
                     /tool="antismash"
     misc_feature    complement(2953..2967)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.48"
                     /tool="antismash"
     misc_feature    3040..3055
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3174..3692
                     /gene="ectA"
                     /locus_tag="S1361_RS10245"
                     /old_locus_tag="S1361_10235"
     CDS             3174..3692
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="S1361_RS10245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10235"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_208031531.1"
                     /transl_table=11
                     /translation="MTAAPADLLIERPAPTDGAALWRLAKDSKTLDLNSSYSYVLWCRD
                     FAGTSAVARGADGEPVGFVTGYLRPDRPRTLLVWQVAVDPGHRGLGIAARLLDGLTARL
                     IAERGITELETTITPGNTASERLFTSFAERHGAGVTREVLFPADLFPDGPHDPEVLHRI
                     GPLTDFTAH"
     gene            3706..4977
                     /gene="ectB"
                     /locus_tag="S1361_RS10250"
                     /old_locus_tag="S1361_10240"
     CDS             3706..4977
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.6e-72.
                     Score: 236.3. Matches aSDomain:
                     nrpspksdomains_S1361_RS10250_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="S1361_RS10250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10240"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_208031532.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1e-76, bitscore:
                     257.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRSFLRAFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFRPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRTRGEQIEQAL
                     ISITEENLADVKEYRGRGLVWGLEFRDKERASRVARRAFDLGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLSVLARAVRETA"
     aSDomain        3790..4803
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_S1361_RS10250_Aminotran_3.1"
                     /evalue="1.60E-72"
                     /label="S1361_RS10250_Aminotran_3.1"
                     /locus_tag="S1361_RS10250"
                     /protein_end="366"
                     /protein_start="28"
                     /score="236.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRSFLRAFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFRPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRTRGEQIEQALISITEENLADVKEYRGRGLVWGLEFR"
     gene            5001..5405
                     /locus_tag="S1361_RS10255"
                     /old_locus_tag="S1361_10245"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="S1361_RS10255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10245"
                     /product="ectoine synthase"
                     /protein_id="WP_208031533.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.8e-53, bitscore:
                     177.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRAKSGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCTRGEAELTDHEAGKTYTITPGTMYLLDGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5412..6305
                     /gene="thpD"
                     /locus_tag="S1361_RS10260"
                     /old_locus_tag="S1361_10250"
     CDS             5412..6305
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="S1361_RS10260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10250"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_208031534.1"
                     /sec_met_domain="PhyH (E-value: 2.3e-39, bitscore: 134.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAIVTDLYPTRGTGEVTVPRQDPVVWGSPDTPGPISATDLQAY
                     ERDGFLAVDQLITPDEVEVYRRELDRLVADPAIRADERSIVEPKSQEIRSVFEVHRISE
                     VFAKLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPHM
                     RTVSVSIALTENYDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALT
                     ALASEYGIRLFTGKAGSATWFDCNAMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAAP
                     VRRPEFIGARDFTPVR"
     gene            complement(6369..7430)
                     /locus_tag="S1361_RS10265"
                     /old_locus_tag="S1361_10255"
     CDS             complement(6369..7430)
                     /NRPS_PKS="Domain: Aminotran_5 (20-347). E-value: 1.1e-24.
                     Score: 79.0. Matches aSDomain:
                     nrpspksdomains_S1361_RS10265_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="S1361_RS10265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10255"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_208031535.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.1e-19, bitscore:
                     67.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTETFESLVRAEFSPESSYLNTASNGLLPARTVAALQEAALLR
                     AEGRPLTPLYEDVEASRAAYARLAGVPAGRVAAGASVAAHTGVIAASLPAGAEVLTAED
                     DFTSVLNPFHVRGDLKVRTVPLERIAESVRPGTALVAVSAAQSADGRIADLPALREAAH
                     AHGARTYVDFSQAAGWLPMDADAYDFTAAVTFKWLLGPHGAAFLTVPEDFGGLTPLLAG
                     WVAAERPWDSCYGPVTELAHSARRFDLTHALFTFAGLRRSLELIEELGVDAVHAHDVAL
                     ADRFREGLAGLGHEPVPAPGSAIVSVPGLGFRQPELSEAGIEVSDRAGNLRAAFHLYTT
                     AADVDRLLDALSG"
     aSDomain        complement(6390..7370)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_S1361_RS10265_Aminotran_5.1"
                     /evalue="1.10E-24"
                     /label="S1361_RS10265_Aminotran_5.1"
                     /locus_tag="S1361_RS10265"
                     /protein_end="347"
                     /protein_start="20"
                     /score="79.0"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVAALQEAALLRAEGRPLTPLYEDVEASRAAY
                     ARLAGVPAGRVAAGASVAAHTGVIAASLPAGAEVLTAEDDFTSVLNPFHVRGDLKVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLPALREAAHAHGARTYVDFSQAAGWLPMD
                     ADAYDFTAAVTFKWLLGPHGAAFLTVPEDFGGLTPLLAGWVAAERPWDSCYGPVTELAH
                     SARRFDLTHALFTFAGLRRSLELIEELGVDAVHAHDVALADRFREGLAGLGHEPVPAPG
                     SAIVSVPGLGFRQPELSEAGIEVSDRAGNLRAAFHLYTTAADVDRL"
     gene            complement(7526..8245)
                     /locus_tag="S1361_RS10270"
                     /old_locus_tag="S1361_10260"
     CDS             complement(7526..8245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="S1361_RS10270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10260"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_208031536.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDQVEVVHRSFELDP
                     GRAKGDVQPVITMLTRKYGMSEAQAEAGEDNLGAQAAAEGLDYRTRGRDHGNTFDMHRL
                     LHFAKEHGLQDRLIQLLYRANFAEERSLFTEGDERLVELAAEAGLDAEAARAVLADPAA
                     YADEVRADEREAARLGATGVPFFVLDRTYGVSGAQPAEVFTRALTQAWGERSPLQLVDQ
                     RDAAACGPDGCAVPQHG"
     gene            complement(8321..8827)
                     /locus_tag="S1361_RS10275"
                     /old_locus_tag="S1361_10265"
     CDS             complement(8321..8827)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329308.1"
                     /locus_tag="S1361_RS10275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_10265"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_208031537.1"
                     /transl_table=11
                     /translation="MISDRAKGARVIRTALPAEAERIAAVHSRARSTYYPDGLPDDGID
                     WTAAWRGAVQRTDGQVLCVVEDGAITGIASFRPPEDGPADTVKLYQFHVDPDRWRRGTG
                     TALHAACVEQWRAEGRSRAVLDVHVDNRRAQAFYARQGWLPDPANPPADDDHHLFLHFA
                     VPPRE"
     gene            complement(8919..9345)
                     /locus_tag="S1361_RS10280"
                     /pseudo=""
     CDS             complement(8919..9345)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448154.1"
                     /locus_tag="S1361_RS10280"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAGWELPEPPFALRAHGPDGDWAYEDGVLTGRAGAAPEGDFRLVA
                     RVTVGFRAAFDAGVLYVHAGERAWAKLCLEYSPDVPTVCTVVTRGHRAALHPRCAEGDR
                     RGARRVHDPVAGGGGVRDDVRLHRVQVRRAGRPARRQL"
     misc_feature    9958..9972
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 gtagcacagg cccgcgacca ggatcggcgt ggagttggcg gtcgtactgg cgaggtcacg
       61 gccgaacttg gacagttccc gctcctccag ggtgacgccg aggaagagca ccagcgagga
      121 gtccttgaac agcaggacga gttcgttggt gagcggcggc aggatgatcc ggaaggcctg
      181 cgggacgatg atggagacca tggccttcgc gggcgagaag cccagcgaac gcgccgcctc
      241 catctgtccc ttgggcaccg cctggatgcc ggcccggatg gtctccgcca tgtaggcggc
      301 cgagaccaga ccgagcgcga tggcgacctt gccgtaggtg ccgcccggga tctccgtgcc
      361 cgggaaggcg agcgggacgg ccaccccgac gaagatgaag atcagcaggg cgggcaggcc
      421 gcggaagatc tcgatgtaga cgcccgccag ccagcggtac gggccgacgg aggacagccg
      481 catcagcgcg atgaccatgc cgaggacgag gccgacgacg aagccggaca gcgtgtagag
      541 cacggtgttc ttcagcgcca gcgtgatgac gtccgggaac atctgccggg cgatgtcggc
      601 ctgggcgaac tggttctgca gccggtccca gtccgccgtg accgcgaagg cgatcacggc
      661 ggcgacgaag acggcgtact gcacaccccg cgagatgctg cgcttctggc gccgggtcag
      721 gccctttttc ttcggttgca gtcgtacgtc cgtctcggtc atgaggcggc gggggaggcc
      781 gcggacgggt tgtacgggcc gatccactgc tcgtagatct tcttgtaggt gccgtcggcc
      841 ttggcgtcct tgagggcctt gttgatggcg tcgagcagcg ccttgttgcc cttcttgacg
      901 gtgaagccgt actgctcacc ggtcttgagg ttgtcgacga ccttgaactt gtccgcgttg
      961 gccttgtcct tcagccagcc ctggacgacc gggtagtcga tgacgacggc ctggacctgg
     1021 ccggtgcgca ggccgttgag gacggcgtcg gaggactcga aggagatcgg gtcgtagccc
     1081 ttgctcttga cgtagtcctc gccggtggtc tgggcctggg cgccgagctt cttgcccttg
     1141 gccttgacgt cggcgagcga gtcgataccg ctgctcttgt cgaccaggac ggcctgggtg
     1201 gcctcgaagt aggggtcgga gaagtcgacg ttcttcttgc gctcggcggt gatggtcatg
     1261 ccggcggcgg ccaggtcgca ctcgccggag ttcaggaagg cgccggtctt gaagttctcg
     1321 aagggcgtgt cgaggatctg ctgcttgacg ccgaggtcct tggcgacgag gtcgatcagg
     1381 gagacgtcga agccctgcac cttgccgttg atctccgact ggaacggcgg gtagggcagg
     1441 tgggtgcagg tggtcagctg accggccttg gcgaggtgga cgcccttgac ggtggtcttg
     1501 ccgctccccg agtcgctgga ggagcagccg gccgcgacga gcacgagtgc ggcggtcgcg
     1561 gtggtggcgg ccaggaggcg ggtccggcgc cctgcgagcg ttttcatggg ggagatctcc
     1621 tgtgggggaa ctgaggcttc tgaaaggaag ggtttgagtc tctcaaatca aaccgatggc
     1681 cacgaggcct ttcgacctaa gatcggtgga gtcgacccgc cgagagcgaa gagagcaccg
     1741 ccgtgaccca ccccttcctc gacctgcccc cgctcggcgc cgagcgcttc gccgccatcg
     1801 aggacggggt ggcccggctg ctggacaccc ggcaggacgt gctgatcacg cagggcgagg
     1861 cgctgctgcc gctggagggc gcgatccgcg cggcggccgg tccgggcacc gtggccctga
     1921 acgtgatcac cggcccgtac ggccagacct tcggcgactg gctgcgcgac gccggcgcca
     1981 cggtgcacga cctctgcgtc cccttccaca cggcggtgac cgccgagcag atccgggagg
     2041 cgttcgccga gcacccggag atcgacttcg tgtcgctggt gcacgcagag gcggccaccg
     2101 gcaacaccaa cccggtggcg gagatcggcg aggcggtacg ggcacagggt gccctcttct
     2161 acctggacgc ggtggcctcg gtgggcgcgg agccggtgct gccggatgcg tggggcgtcg
     2221 acctgtgcgt gatcggcgcg cagaaggcga tgggcggccc ggcgggggtg tcggcgatct
     2281 cggtgagcga gcgggcgtgg gcccggatgg ccgccaaccc gaacgccccg cgccgctcct
     2341 acctctcgct gctggactgg aaggagcgct ggatcgacgg cggccgcaag gcactgccgc
     2401 acgctccggc ccagctggag atgctggcct tggaggcgtg cctggagcgg atcgagtcgg
     2461 ccggcctgca gacggtgatg gaccggcacc ggagcgccgc gctcgccgcg cgggcgggtg
     2521 cggtggcact gggcgggggg ctggagccgt acgtgtacca ggagcgggag gccgcgcccg
     2581 tcgccacgac cctgcgggtg ccgtccgggg tggtggcctc ggatctggtg gcccgcgccc
     2641 tggcgaccga cccggcactg ccactggcgg ccggtggcgg tgccctggcg aaggagatga
     2701 tccgcgtcaa ccactacggc cccgacgcga cgcgggatgc ggtccgcgac tccctggcgg
     2761 cgctgggcgc ggcgctcacc gagcagggcg tgccggtgga cccggccgcc gcccggcggg
     2821 cggtggagga cgcatggcgg tgaacagcga ccggtagtca accgggaaca cacggaaaac
     2881 ggctcgcgca caccgcgcgg gccgttttcg catctcgaat tccaattact ttcacaaact
     2941 ccgccaattc tgcggaccgt agcttcccgt cctcttctgc ccgctttttg cagagctaaa
     3001 ccgggaagtt tgctggccgg tttccggcga tttcctctct gtgacacagt ccacagcaag
     3061 ggtgatttgt accagttttc ctgggggtat tccggacata tcgccgaacc gttgcgcgat
     3121 cccggcaacc actcccgtgc attttcgaat tcgcctcgct aaattcgtgc cgcatgactg
     3181 ccgcaccagc agacctgctc atcgaacggc cggcgccgac cgacggagcc gcgctctggc
     3241 gtctcgccaa ggactcgaaa accctcgatc tgaactcctc ctacagctac gtgctgtggt
     3301 gccgggactt cgccggcacc tcggcggtgg cgcgcggtgc ggacggggag ccggtcggct
     3361 tcgtcaccgg atacctgcga ccggaccgcc cccgcaccct gctcgtctgg caggtggccg
     3421 tcgaccccgg gcaccggggc ctcggcatcg ccgccaggct gctggacggg ctgaccgccc
     3481 ggctcatcgc cgaacgcggc atcaccgagc tggaaaccac catcacgccc ggcaacaccg
     3541 cctccgagcg gctgttcacc tcgttcgccg agcggcacgg cgccggggtc acccgcgagg
     3601 tgctgttccc cgccgatctg ttcccggacg gcccgcacga ccccgaagtc ctccaccgca
     3661 tcggcccgct gaccgacttc accgcccact gaggagcgat tcaccgtgac catcacccag
     3721 cccgacctga gcgtcttcga gaccctggag tccgaggtgc gcagctactg ccgtggctgg
     3781 cccaccgtct tcgaccgtgc gcagggcagc cggatgtacg acgaggacgg tcatgcgtac
     3841 ctcgacttct tcgccggtgc cggatcgctg aactacggtc acaacaaccc ggtgctcaaa
     3901 cgggcgttga tcgactatct ggagcgggac ggcgtcacgc acggtctgga catgtcgacc
     3961 acggcgaagc ggtcgttcct gcgggcgttc caggacctgg tgctgcgtcc gcgtgacctg
     4021 ccgtacaagg tcatgttccc gggtccgacg ggcaccaacg cggtggagtc ggcgctgaag
     4081 ctggcgcgga aggtgaaggg gcgcgaggcg atcgtgtcgt tcaccaacgc cttccacggc
     4141 atgtccctgg ggtcgctggc ggtgaccggc aacgcgttca agcgggccgg tgccggtatc
     4201 ccgctggtgc acggcacgcc gatgccgttc gacaactact tcgacggcac ggtcccggac
     4261 ttcctgtggt tcgaacggct gctggaggac caggggtcgg gtctgaacag gccggccgcg
     4321 gtgatcgtgg agacggtcca gggcgagggc ggcatcaacg tcgcccgccc ggagtggctg
     4381 cgcgcgctga aggagctgtg cgagcggcag gacatgctgc tgatcgtcga cgacatccag
     4441 atgggctgcg gccgtaccgg cgccttcttc tccttcgagg aggccggcat cgtcccggac
     4501 atcgtcaccg tctcgaagtc gatcagcggc tacgggctgc cgatgtcgct gtgcctgttc
     4561 aggcccgaac tggacgtgtg ggagccgggc gagcacaacg gcaccttccg cggcaacaac
     4621 cccgccttcg tcaccgccac cgccgccctg gagacctact gggccgacgg ctccgccatg
     4681 gagaagcaga cccgcacccg cggcgagcag atcgagcagg ccctcatctc gatcaccgag
     4741 gagaacctcg ccgacgtgaa ggagtaccgc ggccgcggtc tggtgtgggg gctggagttc
     4801 cgcgacaagg agcgggcctc gcgggtcgcg cgccgcgcct tcgacctggg gctgctcatc
     4861 gagacgtccg gccccgaggg cgaggtcgtc aagctgctgc ccgccctcac catcaccccc
     4921 gacgaactgg acgagggcct gagcgtcctc gcccgcgccg tccgggaaac cgcctgacag
     4981 cgaggaggaa acgcaccccc atgatcgtcc gatccttcaa ggacatcgaa ggcaccgacc
     5041 ggcacgtcag agccaagtcc ggcacctggg agagcaagcg gatcgtcctc gccaaggagc
     5101 gggtcggctt ctccctgcac gaaacgattc tctacgccgg gaccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca cccggggcga ggccgaactc accgaccacg
     5221 aggccgggaa gacctacacc atcacccccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgcgtcaag gaggacttcc actgcatctg cgtgttcaac ccgccggtca
     5341 ccggccggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgagacac gatgaccact gccatcgtca ccgatctgta ccccacccgc ggcaccggcg
     5461 aggtgaccgt cccccgccag gacccggtcg tctggggctc cccggacacc ccgggcccca
     5521 tctcggccac cgacctccag gcctacgagc gggacggctt cctcgccgtc gaccagctga
     5581 tcaccccgga cgaggtcgag gtgtaccggc gtgagctgga ccggctggtc gccgacccgg
     5641 cgatccgcgc cgacgagcgc tccatcgtgg agccgaagtc ccaggagatc cgctcggtct
     5701 tcgaggtgca ccggatcagc gaggtgttcg cgaagctggt ccgcgatccg cgcgtggtcg
     5761 gccgggcccg gcagatcctc ggttcggacg tgtacgtcca ccagtcccgg atcaacgtca
     5821 agccgggctt cggcgcgagc ggcttctact ggcactcgga cttcgagacc tggcacgccg
     5881 aggacggcct gccccacatg cgcacggtgt ccgtctcgat cgcgctgacc gagaattacg
     5941 acaccaacgg cggcctgatg atcatgcccg gttcgcacag gacgttcctc ggctgtgccg
     6001 gcgccacgcc gaaggacaac tacaagaagt ccctccagat gcaggacgcg ggcacgccgt
     6061 cggacgaggc gctgaccgcg ctggcgagcg agtacggcat ccggctgttc accggcaagg
     6121 ccggctctgc gacctggttc gactgcaacg ccatgcacgg ctccggcgac aacatcacgc
     6181 cgttcccgcg cagcaacgtc ttcatcgtgt tcaacagcgt ggagaacacg gcggtggagc
     6241 cgttcgcggc gccggtgcgc cggccggagt tcatcggggc gagggacttc accccggtgc
     6301 gctagccgcc ccccgaacac cgggccgggg cctcctcgtg tgggacggga ggccccggcc
     6361 cggtcatgtc agccggacag cgcgtccagc agccggtcca cgtccgcggc cgtggtgtag
     6421 aggtggaagg ccgcgcgcag gttgccggcg cggtcggaga cctcgatgcc cgcctcgctc
     6481 agctccggct gccggaagcc gagtccgggg acggagacga tcgccgagcc gggggcgggc
     6541 accggctcgt ggccgaggcc ggcgagcccc tcccggaacc ggtcggcgag ggcgacgtca
     6601 tgggcgtgca cggcgtccac gccgagttcc tcgatcagct ccagcgagcg ccgcagcccc
     6661 gcgaaggtga acagggcgtg ggtgaggtcg aaccgccggg cggagtgggc gagttcggtg
     6721 accgggccgt agcagctgtc ccacggccgc tccgccgcga cccagcccgc gagcagcggg
     6781 gtcagcccgc cgaagtcctc cgggacggtg aggaaggccg cgccgtgcgg cccgagcagc
     6841 cacttgaagg tgacggcggc ggtgaagtcg taggcgtccg cgtccatcgg cagccagccg
     6901 gcggcctgcg agaagtcgac gtaggtgcgg gcgccgtggg cgtgggcggc ctcgcgcagg
     6961 gcgggcaggt cggcgatccg cccgtcggcg gactgggcgg cgctgaccgc gaccagcgcg
     7021 gtgcccgggc ggacggactc ggcgatccgc tccagcggga ccgtgcggac cttgaggtcg
     7081 ccgcggacgt ggaaggggtt gagcacggag gtgaagtcgt cctcggcggt gaggacttcg
     7141 gcgcccgcgg gcagcgaggc ggcgatcacg ccggtgtgcg cggcgaccga ggcgcccgcc
     7201 gcgacccggc cggccggcac gccggcgagc cgggcgtacg cggcccggct cgcctccacg
     7261 tcctcgtaca gcggggtcag cggccggccc tcggcccgca gcagcgccgc ctcctggagg
     7321 gcggcgacgg tgcgggcggg cagcaggccg ttgctcgcgg tgttgaggta gctgctctca
     7381 ggactgaact cggcgcggac gaggctctcg aacgtctccg tggtctccat gggtccactc
     7441 tgcggcccag ggaactaccc gtccattgcg aacttttacg tggttgctct tagcggcact
     7501 tatacgaccg ctcccacctg cggtttcagc cgtgctgcgg caccgcgcac ccgtccgggc
     7561 cgcaggctgc ggcgtccctc tggtcgacga gctgcagcgg ggagcgctcg ccccaggcct
     7621 gggtcagcgc ccgggtgaag acctcggcgg gctgggcgcc ggagacgccg tacgtccggt
     7681 cgaggacgaa gaagggcaca ccggtggcgc cgagccgggc ggcctcgcgc tcgtcggcac
     7741 ggacctcgtc ggcgtacgcg gccgggtcgg cgagcaccgc gcgggcggcc tcggcgtcca
     7801 gaccggcctc ggcggccagc tccaccaggc gttcgtcgcc ctcggtgaac agggaccgct
     7861 cctcggcgaa gttcgcccgg tagaggagct ggatcagccg gtcctgcagg ccgtgctcct
     7921 tggcgaagtg cagcaggcgg tgcatgtcga aggtgttgcc gtggtcgcgg ccccgggtgc
     7981 ggtagtccag gccctcggcg gcggcctggg cgcccaggtt gtcctcaccg gcctcggcct
     8041 gtgcctcgct catgccgtac ttcctggtca gcatggtgat gaccggctgg acgtcgccct
     8101 tggcacggcc cgggtccagc tcgaaggagc ggtgcaccac ctcgacctgg tcgcggtgcg
     8161 ggaaggcagc cagcgccttc tcgaagcggg ccttgcccac gtagcaccag gggcaggcga
     8221 tgtcgctcca gatctcgacg cgcatgtctt cggctctctc cgggtcgtac gggtgcgggg
     8281 actgtccccg accggtacgt gaacgttcaa gcaggctgtt tcattcccgg ggcggcacgg
     8341 cgaagtgcag gaagaggtgg tggtcgtcgt ccgcgggcgg gttcgcgggg tccgggagcc
     8401 agccctggcg ggcgtagaag gcctgggcgc gccggttgtc cacgtggacg tcgaggacgg
     8461 cccggctccg gccctcggca cgccactgct cgacgcaggc agcgtgcagg gccgtaccgg
     8521 tgccgcggcg ccagcggtcg gggtcgacgt ggaactggta cagcttgacc gtgtcggcgg
     8581 ggccgtcctc cggcggccgg aacgaggcga tgccggtgat cgcgccgtcc tccacgacgc
     8641 acagcacctg cccgtcggtg cgctggacgg cgccgcgcca agcggcggtc cagtcgatgc
     8701 cgtcgtccgg gaggccgtcg gggtaatagg tcgagcgggc ccggctgtgc actgcggcga
     8761 tccgctcggc ctcggcgggc agggcggtgc ggatcacccg ggcgcccttg gcacggtcac
     8821 tgatcatgca acggaggacg cggccgcccg aggcccggtt ccgtggcggc ggagccgggc
     8881 ccggcaggcg ctcggggcgg gccggtgcgc cggagcggtc agctgccgtc tggcaggccg
     8941 cccggcgcgc cggacctgaa cgcgatgcag tcgtacgtca tcgcgcaccc ctcccccacc
     9001 ggcgactggg tcatgaaccc gacgagcacc gcgtcggtcg ccttctgcac accgagggtg
     9061 aagagccgca cggtgtcccc gggtgaccac cgtgcacacg gtgggcacgt ccggggagta
     9121 ctccaggcag agcttggccc aggcccgctc gccggcatgg acgtacagca ccccggcgtc
     9181 gaaggcggca cggaacccca cggtgacccg tgcgacaagc cggaagtccc cctcgggcgc
     9241 agccccggcc cgtccggtga gcacgccgtc ctcgtacgcc cagtcgccgt ccggcccgtg
     9301 ggcccgcagt gcgaagggcg gttcgggaag ctcccacccc gccacggcgc taccgctcca
     9361 ggaccccgtt gaaccgccgg ggaagcccca aggggttgtc atccttcagc tcggccggca
     9421 acagggcctc gggcgcgttc tggtacacga cgggccgaag ccaccgctcg acagccgtac
     9481 ctcccacgga cgtggacgtg gaggtggtcg cggggtacgg ccccccgtgg tgctgggccg
     9541 gcgccacggc gacacccgtc ggccagccgt tgaccagcac ccgtccggcc agcggggtca
     9601 gctcggccag cagctccggc ccgcgcccct gccccgcggc ctcctccgcg gacacctgga
     9661 cggtcgccgt gaggttgccc ggcagtcgcg acagcacagc cgtcgcctcg gcctcggacg
     9721 cgtagcgggc caccacggtg accggcccga agcactcctc cagcagcagg tcgtacgccc
     9781 cttcctcggt cagcctctcc gccggcaccg tgaggaaccc ggcgctgacc gagtgttcgc
     9841 cgcccgcgcc cggggtgacc ggcgcgtcga cgtccgggag ctgcgcgcgc tcggccacgc
     9901 ccgcgatgaa gtcgtcccgc atgcggtggt cgagcagcac cccggcgtcc gtgtcgccga
     9961 ccgcgtcggc cagggacttc accagccggt cgcccgccgc gccggacggc accagcacca
    10021 gaccgggctt gacgcagaac tggcccacac ccagggtcat cgagccggcg agcccgccgc
    10081 cgatctcctc cgcccgctcg gccgcggcgg cctccgtgac caggaccggg ttgagcgaac
    10141 ccagctcgcc gtggaacggg atcggcaccg gacgcgctgc ggctgcgtcg aagagcgccc
    10201 gtccgccccg caccgagccg gtgaagccgg ccgcggcgac cagcgggtgc ccgatcagct
    10261 cgacgcccgc ctcgaagccg tgcacgagac cgagcacgcc ctcggggatg ccgcgttcgg
    10321 cggccgcccg gcgcagcacc ttggcgacca gctcggacag gcccgggtgg tcggggtggg
    10381 acttgaccac gaccgggcag cccgc
//
