LOCUS       NZ_CP071839            10381 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Streptomyces cyanogenus strain S136 chromosome, complete genome.
ACCESSION   NZ_CP071839
VERSION     NZ_CP071839.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces cyanogenus
  ORGANISM  Streptomyces cyanogenus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Kalinowski,J.,
            Fedorenko,V., Yushchuk,O. and Ostash,B.
  TITLE     Complete genome sequence of Streptomyces cyanogenus S136, producer
            of anticancer angucycline landomycin A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773899)
  AUTHORS   Hrab,P., Ruckert,C., Busche,T., Ostash,I., Yushchuk,O.,
            Kalinowski,J. and Ostash,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Genetics and Biotechnology, Ivan Franko
            National University of Lviv, 4 Hrushevskoho St, Lviv, Lviv region
            79005, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Newbler v. 2.8; Canu v. 1.8; Unicycler v. 0.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 974878.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017526105.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 09:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,747
            CDSs (total)                      :: 7,656
            Genes (coding)                    :: 7,375
            CDSs (with protein)               :: 7,375
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 129 of 281
            Pseudo Genes (incomplete)         :: 239 of 281
            Pseudo Genes (internal stop)      :: 25 of 281
            Pseudo Genes (multiple problems)  :: 104 of 281
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:56:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 735318
            Orig. end                         :: 745699
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bohdan Ostash, 4
            Hrushevskoho St., Lviv, 79005, Ukraine.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10381
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10381
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10381
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5381](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5381
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    285..305
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.06"
                     /tool="antismash"
     misc_feature    390..404
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            complement(<577..820)
                     /locus_tag="S1361_RS38905"
                     /pseudo=""
     CDS             complement(<577..820)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134699.1"
                     /locus_tag="S1361_RS38905"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="aldo/keto reductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGLGATPLSIEHRPDERRAIATVHAALDAGVTLIDPPTAVTGTPA
                     RQATTSC"
     gene            complement(967..2034)
                     /locus_tag="S1361_RS03230"
                     /old_locus_tag="S1361_03225"
     CDS             complement(967..2034)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="S1361_RS03230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="S1361_03225"
                     /product="hypothetical protein"
                     /protein_id="WP_208030328.1"
                     /transl_table=11
                     /translation="MSGTRQRTFHGETGTRAVVLRLWRNIGCYGLFNLALLLFIGVLVA
                     DGGPVVLPLLVLTLFTVGVLRMVWLVRFTLRIRIKEIGDVVPDLVASGTRFCLILRPFG
                     SAGHIIVRSGRGRRSLALGGMKVTSTMEQVISRAWSDAYASEAYAVVDPGLALAPGGLV
                     YMRAEHTAWKEPVGRLIEAAHSIVLILPPHQEIRTSTWWEVRKIMELQRLERLTVVLPP
                     YDVKGSGHAQARRELCKLLAVVEDPLCLVHLAEGADPVVNSERVDYYARKLLPDKTLGV
                     HFFRNQGGVLQHRDDGPVMFSFSTGVDSKSLILRRKSKVSESTYRPLLLAAYRSDAEEL
                     PPERHRTTKRRTTAD"
     misc_feature    complement(1873..1875)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2178..2669)
                     /locus_tag="S1361_RS03235"
                     /old_locus_tag="S1361_03230"
     CDS             complement(2178..2669)
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (106-139). E-value:
                     0.0017. Score: 11.1. Matches aSDomain:
                     nrpspksdomains_S1361_RS03235_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002033.1"
                     /locus_tag="S1361_RS03235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03230"
                     /product="hypothetical protein"
                     /protein_id="WP_208030329.1"
                     /transl_table=11
                     /translation="MTTIAVTGHMDLAADSVPAIRAALDGLLRRYARDGALVGVSCIAE
                     GSDSVFAQSVLANGGRLVAIIPSRDYRRTVVGPRHAPLFDHLVEAADEVVVMPHETADR
                     AAYDAANAALLEHADRLVAVWNGEPPSGQGGGTADVVLQARAQGMPVDVVWPEEARRTG
                     "
     aSDomain        complement(2253..2351)
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_S1361_RS03235_PKS_Docking_Cterm.
                     1"
                     /evalue="1.70E-03"
                     /label="S1361_RS03235_PKS_Docking_Cterm.1"
                     /locus_tag="S1361_RS03235"
                     /protein_end="139"
                     /protein_start="106"
                     /score="11.1"
                     /tool="antismash"
                     /translation="YDAANAALLEHADRLVAVWNGEPPSGQGGGTAD"
     gene            complement(2748..3260)
                     /locus_tag="S1361_RS03240"
                     /old_locus_tag="S1361_03235"
     CDS             complement(2748..3260)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068609.1"
                     /locus_tag="S1361_RS03240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03235"
                     /product="hypothetical protein"
                     /protein_id="WP_208030330.1"
                     /transl_table=11
                     /translation="MAGIDDRLWNEGVSPSDFCDGTAKYQATILEQYKLCVEMADRVSS
                     RRNLTNTFFLTLNTAVVGALAAGSGAGWPRSSAWVLVAGLLIVLAQCLAWYVMVRSYRQ
                     LNAAKYAVIGAFEAKLPAYAYSRAEWGALGEGRDWRTYMPLTHVEQWVPVVFALSYLVA
                     FGAVVTR"
     gene            complement(3467..4663)
                     /locus_tag="S1361_RS03245"
                     /old_locus_tag="S1361_03240"
     CDS             complement(3467..4663)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662975.1"
                     /locus_tag="S1361_RS03245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03240"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_208030331.1"
                     /transl_table=11
                     /translation="MKVVCVGGGPAGLYLAILLKRQDPSHDITVHERNPEGSTYGWGVT
                     YWRGLLDKLQEHDPESARAVEEHSVRWTDGVARVGDLTTRHRGDEGFGIGRHRLLGLLA
                     DRARALGVRLEFESEPTEPPAGADLVVAADGVHSALRTRAAGHFGTRVTAGRNHYIWLG
                     TTQVFDAFTFAFTETEHGWIWAYGYGYSGDHSTCVVECSPQTYTGLGLDRADEGEGLAL
                     LGKLFAGVLDGHPLIARSGASWQTFRTLTNRTWHHGNVVLLGDAAHTTHYSIGAGTTLA
                     LEDAMCLAGALRAHAVPEALAAYERRRRTELLSVQSAARYSAQWYENLPRYIHLPPHRM
                     FALLGQRHSPLLPYVPPQLYYGLDKAAGQLEPLRRLKRWLGPRAARALHARSSARPDQ"
     misc_feature    complement(4897..4911)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.0"
                     /tool="antismash"
     misc_feature    4926..4940
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.74"
                     /tool="antismash"
     misc_feature    4953..4966
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: medium, score: 18.02"
                     /tool="antismash"
     gene            5001..5381
                     /locus_tag="S1361_RS03250"
                     /old_locus_tag="S1361_03245"
     CDS             5001..5381
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138613.1"
                     /locus_tag="S1361_RS03250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03245"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_208030332.1"
                     /sec_met_domain="melC (E-value: 1.4e-50, bitscore: 170.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDITRRRALTAAAAVAATASAAALVAPAASAAGHHHTPTTPDSF
                     DEVYKGRRIQGRPASGGGHHHEHGGGYAVFVDGVQLHLMQNADGTWISVVSHYDPVPTP
                     RAAARAAVDELQGAPLLPFPAN"
     gene            5417..6238
                     /locus_tag="S1361_RS03255"
                     /old_locus_tag="S1361_03250"
     CDS             5417..6238
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_030787646.1"
                     /locus_tag="S1361_RS03255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03250"
                     /product="tyrosinase family protein"
                     /protein_id="WP_208030333.1"
                     /transl_table=11
                     /translation="MTVRKNQATLTADEKKRFVAAVLELKRSGRYDDFVRTHNEFIMSD
                     TDTGERTGHRSPSFLPWHRRFLLDFEQALQSVDSSVALPYWDWSTDRTVRASLWAPDFL
                     GGTGRSTDGRVMDGPFAASTGNWPINVRVDGRTFLRRSLGTSVRELPTRAEVESVLSMA
                     TYDMAPYNSSSDGFRNHLEGWRGVNLHNRVHVWVGGQMATGVSPNDPVFWLHHAYIDKL
                     WAEWQRRHPDSAYVPAGNTPNVVDLNDTMKPWNTVRPADLLDHTKHYTFDV"
     gene            complement(6239..6961)
                     /locus_tag="S1361_RS03260"
                     /old_locus_tag="S1361_03255"
     CDS             complement(6239..6961)
                     /EC_number="3.1.26.4"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003967.1"
                     /locus_tag="S1361_RS03260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03255"
                     /product="ribonuclease H"
                     /protein_id="WP_208030334.1"
                     /transl_table=11
                     /translation="MIEDMRERVVAACDGASKGNPGPAGWAWVVSDGDERTPARWAAGP
                     LGRATNNVAELTALEQLLAAVDPDVPLEIRMDSQYAMKAVTTWLPGWKRNGWKTAAGKP
                     VANQDLVVRIDELLGGRSVEFRYVAAHQVDGDPLNDFADRAASQAASVQEAAGSALGSP
                     EPPPSPDTPKAAGAPRARAPRRTGGTASSRTGGAASARTIKAKFPGRCLCGRSYAAGEK
                     IAKNAQGWGHPECRTAEA"
     misc_feature    6974..6988
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.21"
                     /tool="antismash"
     gene            complement(6996..7790)
                     /locus_tag="S1361_RS03265"
                     /old_locus_tag="S1361_03260"
     CDS             complement(6996..7790)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670773.1"
                     /locus_tag="S1361_RS03265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03260"
                     /product="VOC family protein"
                     /protein_id="WP_208030335.1"
                     /transl_table=11
                     /translation="MAVQPEGTPCWADAMFSDVEGAKRFYGDVLGWTFGESSSEYGNYT
                     QAYTDGKAVAAVVPPMPGQEGQSQWCLYFASPDAAATAEKIRQNGGEVLMEPMRVGEFG
                     TMCLAREPSGAVFGVWQAGTHEGFETTATPGAYCWAEVFTREPDQADAFLSAVFPYRMK
                     EIEDDAVDFRMFDVGEDTVLGRMRMTDDFPPEVPSYINVYFAVDDCDAAVDRAVKLGGI
                     LRFGPMSSPFGRFAALSDPQGANFSVIDITTTEGEMPKVKDV"
     gene            8062..8991
                     /locus_tag="S1361_RS03270"
                     /old_locus_tag="S1361_03265"
     CDS             8062..8991
                     /EC_number="2.7.-.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126200.1"
                     /locus_tag="S1361_RS03270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03265"
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_243769057.1"
                     /sec_met_domain="APH (E-value: 8.1e-35, bitscore: 119.7,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAFHSDTPQHPHPQRMHTDEIAVGAPLVQRLIARRFPQWAALPV
                     RRLASSGTENAMFRLGGDLVVRLPRRPAAAPDVTHEQRWLPRLGPLLPVAVPEPLGTGG
                     PDADFPWPWSVYRWLEGRNPVAGAVAEPELLAGQLGGFVRALRRIDPHDGPAAARGVPL
                     AVRDEPTRAALARLTGRIDTAAVTALWEEALRAPAHAGPPVWAHADLSPGNVLVDGGRL
                     SAVIDFGTAGVGDPAVDLIVAWNLLPATARDAFRAAVGAGDAEWARGRGWALSISLIQL
                     PYYQDTNPALAENSRHVIAEILAEAGTR"
     gene            complement(9005..9493)
                     /locus_tag="S1361_RS03275"
                     /old_locus_tag="S1361_03270"
     CDS             complement(9005..9493)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991876.1"
                     /locus_tag="S1361_RS03275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03270"
                     /product="VOC family protein"
                     /protein_id="WP_208030336.1"
                     /transl_table=11
                     /translation="MTTDGFTTCLWFDGQAEEAAQHYVSVFENSRIGRVVRWGESGPGP
                     AGSVLTVEFTANGQKFVALNGGPEFRFNEAISFQILCADQDEIDHYWTKLTENGGEGGP
                     CGWLKDKYGVSWQVVYAPLLDMIGDPDPEKAARATKAMMGMGKLDAAALEQAYAGEQG"
     gene            complement(9638..10030)
                     /locus_tag="S1361_RS03280"
                     /old_locus_tag="S1361_03275"
     CDS             complement(9638..10030)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117992.1"
                     /locus_tag="S1361_RS03280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="S1361_03275"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_208030337.1"
                     /transl_table=11
                     /translation="MSNPVSDEARVVPLRPAPARPAAPETEPAPREPLWRDLVGEVLRR
                     ERQAQERTLKDVADAARISLPYLSEIERGRKEASSEVLAAAAHALGLGLGDLLSLAHGE
                     LARRTVERRRSAAAPHRPYRGLCLAA"
ORIGIN
        1 ctgcagcgcg cgccgcgcga cgggatggac atgcgcttcc tcggcagcct cgtgcacggc
       61 gagcagccgc atcagccggt ggaagttgcg ggagcgccac ggcccgggaa gcgcggcctt
      121 caggaatccg agcctgatct gccggtgctg gcggacgagc aggtcgacgg cgttctcagc
      181 gcccagggag cgtgtgatcc tcagtgacca ggtgggtgcg gctttcgcgg tcttggccgc
      241 ggccttcccg acgcgcagcg gcatctgcgg cacctgcgcg gcggacttcg tcagtgtgac
      301 catgatccct cctcgcttcg gtcccggcga tcccgggtac ccaccggtgc cacgctcacg
      361 cgcccggcac cctcgcggac gcggcaccga tcacccgtgt cgaaaccctt cagccattgc
      421 ggcgctggtc ccctcccctg cgctgcgcgg cccggcaccg gctcggacag gccctgcccg
      481 acccaccgtg gcggcgaact ccaggaggac ggctcgtgcg gcgcccagga tcggggccga
      541 tcgcgtcgac acccagccgc ttggcggagg attcggcccg tcgccgggcc ggccgcgacc
      601 gcccttggtg gcgatcagga caccggaggt gtcgccgccg taccggccag cgcccgggcg
      661 atcagcagct cgttgtggcc tgcctcgccg gcgtgccggt gacagctgtc ggcgggtcga
      721 tgagcgtgac gccggcgtcg agggcggcgt ggacggtggc gatggcgcgc ctctcgtccg
      781 ggcggtgctc gatggacagt ggcgtggcgc ccagaccgat ggcgctgacg gtcgtgtctc
      841 cgacggtgcg gtactgggtg gcggctgatg ttccttattg ggccggggcc ttcgcccaag
      901 ccctccgcga gcaggcgctg cagcgtgagc accatggcct tgacggtgcc cgtcgaccac
      961 tcgggttcag tccgcggtcg tacggcgttt cgtcgtccga tgcctctcgg ggggcagttc
     1021 ttccgcatcc gaccggtagg ccgcgagcag cagcggccga tacgtggatt cgctgacctt
     1081 gctcttcctg cgcagaatca gggacttgct gtcgaccccc gtactgaagc tgaacatcac
     1141 gggtccgtcg tcccggtgct ggaggacacc gccctgattc ctgaagaaat gtacgcccag
     1201 cgtcttgtcc ggcagcagtt tccgcgcgta gtagtcgaca cgttcgctgt tgacgacggg
     1261 atcggcgccc tcggccaggt ggacgaggca cagcgggtcc tccaccaccg caaggagctt
     1321 gcacagctct cttctcgcct gcgcgtgccc gcttcccttc acgtcatacg ggggcaggac
     1381 cacggtgagc cgctccaggc gctggagttc catgatcttg cggacctccc accacgtgga
     1441 cgtgcggatc tcctggtgcg gcggcaggat caggacgatc gagtgtgcgg cctcgatgag
     1501 ccggccgacc ggttccttcc acgcggtgtg ctcggcccgc atgtagacga gaccgccggg
     1561 ggccagggcg aggccggggt cgaccaccgc gtaggcctcc gaggcgtacg cgtcggacca
     1621 ggcccgggag atcacctgct ccatggtgga ggtgaccttc atcccgccca gggccagcga
     1681 ccgccgtccc ctgccgctgc gcacgatgat gtgcccggcg gacccgaacg ggcgcaggat
     1741 caggcagaac cgcgtgccgg aggcgaccag gtccggtacg acgtcaccga tctccttgat
     1801 gcggatgcgc agggtgaagc gcaccagcca caccatcctg agcacaccga ccgtgaagag
     1861 cgtcaatacc agtaacggca gtacgacggg gccgccgtcg gcgaccagca ccccgatgaa
     1921 cagcagcagc gcgaggttga agagcccgta gcagccgatg ttgcgccaca gccgcaggac
     1981 gacggctcgc gttccggttt ccccgtggaa agtgcgctgc ctcgttccgg acatggggcc
     2041 atcccctccc ccgacgggac gcaacgactg caccggttcc gcccggtgag gtcccgtacg
     2101 agactaccca ccacgttctc ccccgcgcca cggaacgctg cgaagcgccg tcgcagtact
     2161 ggatccggac cccgcggtca gccggtccga cgggcttctt ccggccacac cacgtccacg
     2221 ggcattccct gtgcccgtgc ctggaggacc acgtccgccg tgcctccgcc ctggccgctg
     2281 ggcggctccc cgttccagac ggccacgaga cgatcggcgt gttccaggag ggcggcgttc
     2341 gcggcgtcgt aggcagcacg gtccgccgtc tcgtgcggca tgaccacgac ctcgtcggcg
     2401 gcttcgacga ggtggtcgaa caggggcgcg tggcgtgggc cgaccacggt gcggcggtag
     2461 tcccgggagg ggatgatcgc gaccagccgt ccgccgttgg ccaggacgga ctgggcgaac
     2521 accgagtcgg acccctcggc gatgcacgag acgccgacga gtgcgccgtc gcgggcgtac
     2581 cgccggagca gcccgtccag ggcggcgcgg atcgcgggga cgctgtccgc ggccagatcc
     2641 atgtgcccgg tcaccgcgat ggttgtcatg gaccgtcccc ttcacctcga cagagggatc
     2701 ctatcgaccg cgggccctgg aaccgtcctt gccgctgggc ggtgggctca cctcgtcacc
     2761 acggctccga aggccacgag atacgacagc gcgaagacga ccggcaccca ctgctccacg
     2821 tgcgtcagcg gcatgtacgt gcgccagtcg cggccctcgc cgagcgcacc ccactccgcc
     2881 cgcgagtacg cgtaggccgg gagtttcgcc tcgaaggcac cgatcaccgc gtacttggcc
     2941 gcgttgagct gccggtagga gcgcaccatc acgtaccagg ccaggcactg ggcgagcacg
     3001 atcagcagcc ctgccaccag gacccacgcg gaggagcgcg gccagcccgc gccggacccg
     3061 gcggcgaggg cgccgacgac cgcggtgttg agtgtgagga agaaggtgtt ggtgagattg
     3121 cgccgggaac tgacccggtc ggccatctcg acgcacagct tgtactgctc cagtatcgtc
     3181 gcctggtact tggcggtgcc gtcgcagaag tccgacgggc tcacgccctc gttccagagc
     3241 ctgtcgtcga tgccggccat gccctcatga tggccgaaca cccgttgcgg ccggatcaat
     3301 tccgccgaac gggttccgcc ggactccgca accccgtcga agggcttccg gccgccgcgg
     3361 ccagggctgc accggtcacc accctgactg ctccgcaacc gcccggcttg tgctcgtcga
     3421 cgtgccccgc tgcggcgttc aggcgagctc accccaggta cgagcactac tggtccgggc
     3481 gggccgagga gcgggcgtgc agggcgcggg cggcgcgggg gccgagccag cgcttgagcc
     3541 tgcgcagcgg ctccagttgt ccggccgcct tgtcgaggcc gtagtacagc tgcggcggga
     3601 cgtagggcag cagcggcgag tggcgctggc cgagcagggc gaacatccgg tgcgggggca
     3661 ggtggatgta gcggggcagg ttctcgtacc actgggcgct gtagcgggcc gcgctctgca
     3721 cggacagcag ttcggtccgg cgccggcgct cgtaggcggc gagggcctcg ggcacggcgt
     3781 gtgcgcgcag ggcgccggcc aggcacatgg cgtcctccag ggcgagggtg gtgccggcgc
     3841 cgatggagta gtgggtggtg tgggccgcgt cgccgagcag gaccacgttg ccgtggtgcc
     3901 aggtccggtt ggtgagggtg cggaaggtct gccaggaggc gccggagcgg gcgatcaggg
     3961 ggtggccgtc gaggacgccg gcgaagagct ttcccagcag ggcgagtccc tcgccctcgt
     4021 cggcccggtc caggccgagg ccggtgtagg tctgcgggga gcactcgacg acgcaggtgc
     4081 tgtggtcgcc gctgtagccg tagccgtagg cccagatcca gccgtgctcg gtctcggtga
     4141 aggcgaaggt gaaggcgtcg aagacctggg tggtgccgag ccagatgtag tggttgcggc
     4201 cggccgtgac gcgggtgccg aagtggccgg ccgcgcgggt gcgcagggcg ctgtgcacgc
     4261 cgtcggccgc gacgacgagg tccgcgccgg cgggcggttc cgtcggctcg ctctcgaact
     4321 ccaggcggac gccgagggcg cgggcccggt cggcgagcag gccgagcagc cggtgccggc
     4381 cgatcccgaa gccctcgtcg ccccggtgcc gggtggtcag gtcgcccacg cgggcgacgc
     4441 cgtcggtcca ccggacggag tgttcctcga ccgcgcgcgc cgattccggg tcgtgctcct
     4501 ggagcttgtc gagaagtccg cgccagtacg tcactcccca gccgtacgtg gaaccctcgg
     4561 ggttgcgttc gtggacggtg atgtcatggg acgggtcctg acgcttgagc aggatggcga
     4621 ggtacaggcc cgcgggcccg cctccgacgc agacgacctt cacacgacac ccctgacagt
     4681 caccggacgt ggtcggacgg caccggaggg tagcagggac cgcggccctc ccgatcgcgt
     4741 cagtttggcg tggtgagccg cgccactcgg acgggcgtca gccggaaagg atcatcggaa
     4801 acccgggcgc cccggcaagg aatttcccgt tccggagtcg atcggcccac cgcggcagca
     4861 agatcatctt agttcaactc tgtaggacat gtggttaatt gtccggatca tagccaaccg
     4921 gttccggatg atttcttccg ctccggaacg tccggatagg catgcatggt caccaccgca
     4981 ccactttcag gaggacccgc atgcccgaca tcacccggcg ccgcgcgctc accgcggcag
     5041 ccgccgtagc cgcgacggcc tcggccgccg ccctcgtcgc gcccgccgcg tcggccgccg
     5101 gccaccacca cacgcccacc acccccgact ccttcgacga ggtctacaag ggccgccgaa
     5161 tacagggccg tccggcgagc ggcggcgggc accaccacga acacggcggc ggctacgcgg
     5221 tgttcgtcga cggcgtgcag ctgcacctga tgcagaacgc cgacggcacc tggatcagcg
     5281 tcgtcagcca ctacgacccg gtgcccaccc cgcgcgccgc cgcccgtgcc gcggtggacg
     5341 agctgcaggg cgcaccgctg ctgccgttcc ccgccaactg accgccccca caaggacttc
     5401 cggagcaccg cgcaccatga ccgtacgcaa gaaccaggcc accctcaccg ccgacgagaa
     5461 gaagcggttc gtcgccgccg tcctcgaact caagcgcagc gggcgctacg acgacttcgt
     5521 ccgcacgcac aacgagttca tcatgtcgga caccgacacc ggcgagcgga ccggccaccg
     5581 ctccccgtcc ttcctgccct ggcaccgccg gttcctgctc gacttcgagc aggcgctgca
     5641 gtccgtggac tcctcggtgg cgctgcccta ctgggactgg agcaccgacc gcacggtgcg
     5701 cgcctcgctg tgggcaccgg acttcctcgg cggcaccggt cgcagcaccg acggccgggt
     5761 gatggacggc ccgttcgccg cgtccacggg caactggccg atcaacgtgc gggtcgacgg
     5821 ccgtacgttc ctgcgccgtt cgctcggcac gagcgtgcgg gagctgccca cgcgcgccga
     5881 ggtggagtcg gtgctgtcga tggcgacgta cgacatggcg ccgtacaaca gctcctcgga
     5941 cggcttccgc aaccacctgg agggctggcg cggggtcaac ctgcacaaca gagtgcacgt
     6001 gtgggtcggc gggcagatgg ccaccggtgt ctccccgaac gacccggtgt tctggctgca
     6061 ccacgcctac atcgacaagc tgtgggcgga gtggcagcgc cggcacccgg actccgccta
     6121 tgtgccggcg ggcaacacgc cgaacgtggt ggacctgaac gacaccatga agccgtggaa
     6181 caccgtgcgg ccggcggacc tgctggacca caccaagcac tacacgttcg acgtctgatc
     6241 aggcctcggc ggtacggcac tccgggtggc cccagccctg cgcgttcttg gcgatcttct
     6301 cgccggccgc gtaggaccgg ccgcacaggc agcggccggg gaacttcgcc ttgatggtgc
     6361 gggcggacgc ggccccgccg gtgcgggagg acgcggtccc gccggtgcgg cggggcgccc
     6421 tcgcgcgcgg ggccccggcc gccttcgggg tgtcgggcga cggcgggggc tccggcgagc
     6481 cgagcgcgct gccggccgcc tcctggacgg aggccgcctg gctggcggcg cggtcggcga
     6541 agtcgttcag cgggtcgccg tcgacctggt gggcggccac gtagcggaac tcgacggacc
     6601 ggccgccgag gagctcgtcg atgcggacca ccaggtcctg gttggcgacg ggcttgccgg
     6661 cggcggtctt ccagccgttg cgcttccagc cgggcagcca ggtggtgacg gccttcatcg
     6721 cgtactggga gtccatccgg atctccagcg gcacgtccgg atccacggcc gcgagcaact
     6781 gctccagggc cgtcagctcg gcgacgttgt tggtggcccg gccgagcggg cccgccgccc
     6841 agcgcgcggg ggtccgctcg tcgccgtccg agaccaccca cgcccatccg gccggtccgg
     6901 ggtttccctt cgaagccccg tcgcacgcgg ccaccacacg ttcacgcatg tcctcgatca
     6961 tgccatggcc gcaggcagag ccgttccagg tccggtcaga cgtccttgac cttcggcatc
     7021 tcgccctcgg tggtcgtgat gtcgatcacc gagaagttcg cgccctgcgg gtcgctcagc
     7081 gccgcgaacc ggccgaaggg gctgctcatc ggcccgaagc ggaggatccc gccgagcttg
     7141 acggcccggt cgacggcggc gtcgcagtcg tcgacggcga agtagacatt gatgtacgac
     7201 ggcacctcgg gcgggaagtc gtccgtcatc cgcatccggc cgagcacggt gtcctcgccg
     7261 acgtcgaaca tccggaagtc gaccgcgtcg tcctcgatct ccttcatccg gtacgggaag
     7321 acggccgaga ggaaggcgtc ggcctggtcc ggctccctgg tgaagacctc ggcccagcag
     7381 taggcgccgg gcgtggccgt cgtctcgaag ccctcgtggg tgcccgcctg ccacacgccg
     7441 aacacggccc cgctgggctc ccgggccagg cacatggtgc cgaactcgcc gacccgcatc
     7501 ggctccatca gcacctcgcc gccgttctgc cggatcttct ccgcggtggc cgcggcgtcc
     7561 ggtgaggcga agtagaggca ccactgcgac tggccctcct gtccgggcat cggcgggacg
     7621 acggcggcga cggccttgcc gtccgtgtag gcctgtgtgt agttgccgta ctccgacgac
     7681 gactcgccga aggtccagcc gaggacgtca ccgtagaacc gcttggctcc ctcgacgtcg
     7741 ctgaacatcg catcggccca acagggcgtt ccctcgggtt gcacggccat gatcgaagcc
     7801 ctctcggtca gctggtgtgt cccgctacct cacgctagcc accccgccgt cgccccgctc
     7861 gccgaacgcc cgaagccgca ggaccggggg aagccgggtg cggggcacaa gatcggcgcg
     7921 cggtcaccgc aaccgccacg ggacgctccg cacgcccccg tgacgcaccc ctcgtgacgc
     7981 acacccccgt gacgcacgcc ctcgtgagcc cgtcgaaatc ccgtcgacag cgggggcgtc
     8041 gaggcgctgg gatgagcctc gatgagtgcc ttccacagcg acacaccgca gcacccgcac
     8101 ccgcagcgga tgcacaccga cgagatcgcc gtcggcgccc cgctggtcca gcgcctgatc
     8161 gcccgccgtt tcccgcagtg ggcggcgctg cccgtgcggc gtctggcgtc ctccgggacc
     8221 gagaacgcca tgttccggct gggcggcgac ctcgtggtcc ggctcccccg acgccccgcc
     8281 gccgcgccgg acgtgacgca cgagcagcgc tggctgcccc ggctcggtcc gctgctgccg
     8341 gtggcggtgc ccgagccgct ggggacgggc ggacccgatg cggacttccc gtggccctgg
     8401 tccgtgtacc gctggctgga gggccgtaat ccggtcgcgg gggccgtggc cgaacccgag
     8461 ctgctcgccg ggcagttggg gggattcgtg cgtgcgctgc gccggatcga cccgcacgac
     8521 ggtccggcgg ccgcccgggg cgtgccgctc gccgtccggg acgagccgac ccgggcggcg
     8581 ctcgcccggc tcaccggacg gatcgacacc gcggccgtga cggccctgtg ggaggaggcg
     8641 ctgcgggccc cggcgcacgc gggcccgccg gtctgggcgc acgcggacct gtccccgggg
     8701 aacgtgctgg tcgacggtgg acggctgagc gcggtcatcg acttcggcac cgccggggtc
     8761 ggcgaccccg ccgtggacct gatcgtggcc tggaacctgc tgcccgccac cgcccgggac
     8821 gccttccgtg cggccgtcgg cgccggggac gcggagtggg cgcgcgggcg gggctgggcc
     8881 ctgtcgatct cgctgatcca gctgccgtac taccaggaca ccaacccggc cctggcggag
     8941 aactcccggc atgtgatcgc cgagatcctc gccgaggccg ggacgcggtg acggcgcccg
     9001 ggcctcaacc ctgctcgccg gcgtacgcct gctccagggc cgcggcgtcc agcttgccca
     9061 tgcccatcat ggccttggtg gcgcgcgcgg ccttctccgg gtccgggtcg ccgatcatgt
     9121 cgagcagcgg ggcgtagacg acctgccagg agacgccgta cttgtccttg agccagccgc
     9181 acgggccgcc ctcgccgccg ttctcggtga gcttcgtcca gtagtggtcg atctcgtcct
     9241 ggtcggcgca caggatctgg aaggagatcg cctcgttgaa cctgaactcc ggaccgccgt
     9301 tgagcgcgac gaacttctgg ccgttggcgg tgaactccac ggtcagcacg gaaccggccg
     9361 gaccgggacc ggactcgccc caccggacga cccggccgat gcgggagttc tcgaagacgg
     9421 acacgtagtg ctgcgcggcc tcctccgcct ggccgtcgaa ccagagacac gtggtgaatc
     9481 cgtcggtggt catgagttcc tcctgggacg tcgaacggcg cgtggaacgc ggtcatgggt
     9541 atcgaccgcg ccgcgccgcg aaactcatcg gtcccgtgcg tgcccactcg aacgggtgaa
     9601 ggggctgcgg ccctgagagg cgacggcggg cagccggtca ggcggccagg cagagcccgc
     9661 ggtagggccg gtgcggcgcg gcggcggacc tgcgccgctc gaccgtgcgc cgggccagct
     9721 cgccgtgcgc cagcgacagc aggtccccga gccccaggcc cagcgcgtgg gcggccgcgg
     9781 cgagcacctc ggacgaggcc tccttgcggc cccgctcgat ctcggacagg tagggcagtg
     9841 agatccgggc cgcgtcggcc acgtccttga gcgtgcgttc ctgcgcctgc cgctcgcggc
     9901 gcagcacctc gccgaccagg tcccgccaga ggggctctct gggtgcgggc tcggtctccg
     9961 gcgcggccgg ccgggcgggt gccggccgga gagggaccac tcgggcttcg tcgctcaccg
    10021 ggttgctcat cccgtcagcc tagaagcggt acggtcgggg acaagggccc ggtattgcgc
    10081 cctgagggaa atcgcggccg gtcggcggct cggaccggtc gtttcccgcc agtgcggaac
    10141 ggccggtgca tgcgtcgcgt caccgggggt acccgcgccg tgagcgcctc gacggcatgc
    10201 atggaccgcg ggttcagggg gtacccgggg ttggtgagct gtacgagctg tgccgggcat
    10261 ccggcacatg ctgtcgaggc ggacgtcgaa ccgtgacttt cgtgggagag gtaatgaaca
    10321 ccgccgccgg gatccggtcg gaagcagagg tcatggaaca gaccgagcag gacaggacgg
    10381 g
//
