LOCUS       NZ_VOEC01000063         6659 bp    DNA     linear   CON 11-APR-2024
DEFINITION  Enterococcus hirae strain DMW1-1 DMW1_1_contig_63, whole genome
            shotgun sequence.
ACCESSION   NZ_VOEC01000063
VERSION     NZ_VOEC01000063.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus hirae
  ORGANISM  Enterococcus hirae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Phylogenetic and comparative genomic of wild marine
            animal-associated enterococci from the southern coast of Brazil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2019) Basic Health Sciences Department, Federal
            University of Health Sciences of Porto Alegre, 245 Sarmento Leite
            St., Porto Alegre, RS 90050-170, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 8.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017356505.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 14:47:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 39 of 114
            Pseudo Genes (incomplete)         :: 71 of 114
            Pseudo Genes (internal stop)      :: 29 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:59:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6659
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOEC01000063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..611
                     /locus_tag="FQT05_RS15155"
     source          1..6659
                     /collected_by="Janira Prichula"
                     /collection_date="2013-06-21"
                     /country="Brazil: Tramandai"
                     /db_xref="taxon:1354"
                     /host="Balaenoptera acutorostrata (dwarf minke whale)"
                     /isolation_source="feces"
                     /mol_type="genomic DNA"
                     /organism="Enterococcus hirae"
                     /strain="DMW1-1"
                     /submitter_seqid="DMW1_1_contig_63"
     region          1..6659
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..6659
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6659
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4221:4960](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4222..4960
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     CDS             <5..611
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752121.1"
                     /locus_tag="FQT05_RS15155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_242690857.1"
                     /transl_table=11
                     /translation="PSGNVLMAEEKERNYTYGSNYAYYNVTEYGFEPTGDFTVTSAEAK
                     IVIDTKRVDLKNLLKEVKVNGKELPKTAYTVALDPETEIDTSSLGYRVAKLVVKADRSY
                     GGFSNEIEATYEVVEKGTDGALEDGTEDSMTEDKEITGTDSEKKNSNGDNASGAKNGNL
                     PKTNETKNTIFTVSGVLIVSLAGIILFWRKRKANKNSKK"
     gene            complement(735..956)
                     /locus_tag="FQT05_RS14760"
                     /old_locus_tag="FQT05_14765"
     CDS             complement(735..956)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737647.1"
                     /locus_tag="FQT05_RS14760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14765"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_207496147.1"
                     /transl_table=11
                     /translation="MSIIINLDVMLAKRKMSVTELSEKVGLTMANISILKNGKAKAIRF
                     STLDKICEVLNCQPGDILEYQDTPQMRE"
     gene            complement(965..1438)
                     /locus_tag="FQT05_RS14765"
                     /old_locus_tag="FQT05_14770"
     CDS             complement(965..1438)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /locus_tag="FQT05_RS14765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14770"
                     /product="DUF2975 domain-containing protein"
                     /protein_id="WP_207496148.1"
                     /transl_table=11
                     /translation="MKSKLFFIQLIVSVFCFVPLLLGALLTIQLSTNPTSPFSNWQTTL
                     FILVLYLILLVTLLLNFFLLRLVKIFPSKETFTKKSLKLSSKIRSCLLCITILTLGILP
                     KFYQIADMSDSPGILLIAFVLLFIPFFIYILSSILIDLLKQAIYLKNDYDLTV"
     misc_feature    1528..1541
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.45"
                     /tool="antismash"
     gene            2078..3364
                     /locus_tag="FQT05_RS14770"
                     /old_locus_tag="FQT05_14775"
     CDS             2078..3364
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737645.1"
                     /locus_tag="FQT05_RS14770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14775"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_010719091.1"
                     /transl_table=11
                     /translation="MKLNEESLFILILIGQLYTVFMALGYLRNLLSNKKFQLFSFISVI
                     AYIALYQWINIIATVILWAGLCLSIYIIDRNIDIAIFYPSIILFFYIFIDGTAKYLYKI
                     LGWDISDFFTAVLGTGCLWIVTVFLKKMVIPKIIRDKTTLKIAAIVVFFTIIVYFINIC
                     SDRFSVLARFLVISNELFTVLYGVMSTIIYCVLLFIKKKEFETREQQKEMRHLIEYSEQ
                     IENNYLALRKFKHDYKNILFSIEDYIQTKDIEGLQKYFYQSIKATETIFEENNLPIASI
                     SNLKIREIKSIILSKLYVASQKGVEVNVMIPTPITDIAISTVTLVRMLGIILDNAIEES
                     AFLEKGKIELTIMEEDKNCFFVLMNQCRSSIPKLHELKKVNFTTKKGNQGLGLTNLDEL
                     VHLNENVFLETKIENNRFIQIITISGMRG"
     gene            3367..4113
                     /locus_tag="FQT05_RS14775"
                     /old_locus_tag="FQT05_14780"
     CDS             3367..4113
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750903.1"
                     /locus_tag="FQT05_RS14775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14780"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_207496149.1"
                     /transl_table=11
                     /translation="MLKIFVCDDEIAHRNKLTRMITNYLLMKDYDVQFQFATDDPMMIL
                     SFISKHKTEGIYFLDIDLKTDLNGIELAKKIRDYDPTAKIIFVTTYTDLVYLTFLYKVE
                     ALDYIVKDNMDTFQKRVTECIDVAIERYLNTNLETRPQIWIKNGPIDVKLYVDEIFYFE
                     SSPTPHKLIVYLDNRRIEYIGKIKEVESMYEVFCRCHQSYVVNLMNVKEINKKERVITM
                     ANGAKCLVSTRHLKKVVNQFKKLTSC"
     gene            4222..4818
                     /locus_tag="FQT05_RS14780"
                     /old_locus_tag="FQT05_14785"
     CDS             4222..4818
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737643.1"
                     /locus_tag="FQT05_RS14780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14785"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_207496150.1"
                     /sec_met_domain="AgrB (E-value: 7.2e-23, bitscore: 70.3,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEESISLEEKVSKTISKRIGRQLQLSEIDLTKMEYGLAVLLVIS
                     SKIFFIYCLAGCLHIILPVLTTHLAFTLMRRTTKSFHARDSNVCTALSLFFFVLLPYLV
                     TTFAVDVTRVNFMICSLMICLILWFKGIVIAQKPAKSSLLNGKSQMIVVSLFLTIFGGI
                     QSSNRIRTLLLLGMMISTLLMFVQKRKDEENVKKN"
     gene            4802..4960
                     /locus_tag="FQT05_RS14785"
                     /old_locus_tag="FQT05_14790"
     CDS             4802..4960
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737642.1"
                     /locus_tag="FQT05_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14790"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_207496152.1"
                     /sec_met_domain="TIGR04223 (E-value: 2.1e-10, bitscore:
                     30.0, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKKISSSKLKYMQILATVAIAVATEAIGKGTMCTGIIYEPKVPE
                     ALKQIEK"
     misc_feature    complement(5579..5593)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.65"
                     /tool="antismash"
     gene            5651..6214
                     /gene="amaP"
                     /locus_tag="FQT05_RS14790"
                     /old_locus_tag="FQT05_14795"
     CDS             5651..6214
                     /codon_start=1
                     /gene="amaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /locus_tag="FQT05_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14795"
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="WP_010737641.1"
                     /transl_table=11
                     /translation="MNKGWKIVGILASLFLLTVLLGIALINAPYMMPEQLERFRFFVIT
                     NYYVQQYLFWTAVVFAILVLILLLVVLFYPKSKGTFVLKQKDGKLTLDKKAIEGLVRSQ
                     LHEEEFVGSPKVRVRSTKNKINVHVKGDLKRTSSLIGKTGELMRDIEQQVARVLGTEQN
                     IAVAVTYSGFEDYDSNAHKHARVE"
     gene            6227..6415
                     /locus_tag="FQT05_RS14795"
                     /old_locus_tag="FQT05_14800"
     CDS             6227..6415
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /locus_tag="FQT05_RS14795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14800"
                     /product="DUF2273 domain-containing protein"
                     /protein_id="WP_010719095.1"
                     /transl_table=11
                     /translation="MKEIFSAYKLPIIFGGLGLLLAVLWITVGFFKTLLILIMTAIGAG
                     IGFYLKETKIFDQFFKS"
     gene            6461..>6659
                     /locus_tag="FQT05_RS14800"
                     /old_locus_tag="FQT05_14805"
     CDS             6461..>6659
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176606.1"
                     /locus_tag="FQT05_RS14800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_14805"
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="WP_242690858.1"
                     /transl_table=11
                     /translation="MTSENNHISRGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNI
                     AEKLVNTDNVTAGINAEVGKK"
ORIGIN
        1 atccaagtgg gaatgtcttg atggcagaag aaaaagagcg aaattatact tatggttcca
       61 attatgcgta ttacaatgtg actgaatatg gatttgaacc aacaggagat tttacagtga
      121 catcagcaga agcgaaaata gttatcgata cgaaacgtgt ggaccttaaa aatcttttga
      181 aagaagttaa agtcaatggc aaagagctgc cgaaaactgc ttatacggtt gcattagatc
      241 ctgaaacaga aatcgatact tcctctttgg ggtatcgtgt tgctaaacta gtcgtgaaag
      301 ctgaccgttc ctatggtggt ttttctaatg aaattgaagc gacttatgaa gtggtggaaa
      361 aagggacgga tggtgcttta gaagatggta cagaagatag catgactgaa gataaagaga
      421 tcacaggaac agattcagaa aaaaagaact caaatggaga caatgcaagt ggcgcaaaaa
      481 atggtaattt acccaaaacc aatgaaacaa aaaatacgat ttttactgta tcaggcgtct
      541 tgattgttag tttagcggga atcatcctgt tttggagaaa aagaaaagca aataaaaatt
      601 ctaaaaaata agataatcag ttattaaaaa gtccacgtta gctagactac agctgacgtg
      661 ggcttttttg tcggtcttca gattgattag aaactagtta gctttcattg gtgtcactca
      721 actaaagaaa tgagctactc tctcatttgg ggtgtatctt gatattcgag gatatcacct
      781 ggctggcagt ttaaaacttc gcaaatctta tcgagcgtag aaaaccgaat cgcttttgct
      841 ttaccatttt ttaaaataga gatattagcc atcgtaagac ctactttttc actcagttcg
      901 gtcacgctca tttttcgctt ggctaacatg acatctaaat tgataataat agacattcga
      961 cacctcacac tgttaaatcg tagtcatttt ttaaataaat ggcttgtttg agtaaatcga
     1021 tcaaaatgct tgaaaggatg tagataaaaa aagggataaa gagtaagacg aaagcgatga
     1081 gtaggattcc tggactatcg ctcatatcag ctatttggta aaattttggc aaaataccaa
     1141 gtgtaaggat cgtgatacac aaaaggcaag atcggatttt actgctaagt ttgagactct
     1201 ttttagtaaa agtttccttt gaagggaaga ttttgactaa gcggagcagg aagaaattga
     1261 gcaacaaagt cactagaagg atcaggtaaa ggactagaat aaatagtgta gtttgccagt
     1321 tgctaaatgg acttgtagga tttgtactca attgaatagt taatagcgct cccaagagaa
     1381 ggggaacaaa acaaaaaaca gaaacgatta attggataaa gaatagtttg gatttcataa
     1441 aatgcctcct agatttttat tagtatagca tgatttttat tcattataaa taattattta
     1501 tcgataaacg ataaatagaa ggatggacaa acgccttttt cgataaagaa aaggcgttct
     1561 aacgaatcat attatatttt aaagctgtaa aactattact aaaaatggac tatttctcat
     1621 ttaaacaatg tattccctaa ccactaatac atccttgtaa ccctgaagtt acgccagtgg
     1681 gattaaattt catcaaaaca aatcaaaatc tcgcataaaa tatttatcgc aataaatcaa
     1741 ttataacata tcatttgcaa aaaagatatt taatttagat tacatttgcg atagttttaa
     1801 ataacagaac ttttaagtaa gaaaacgtat cattattatg tttataccct cccaaatcta
     1861 aagtaaatat gctaaaatca caaataacaa cgaagatcgt gattgattgg aaaaattttt
     1921 cctatttttt gttattaaag ttaggtgtac aaataactaa ttgttttatt ttatttggtt
     1981 atgaggagtt gggaaacaaa atacgttagt ataaattgtt atataagtat ggataaaaaa
     2041 ttcagataaa attattaggt tgttaggaga agtttaaatg aaattaaatg aagaaagttt
     2101 atttatccta attttgattg gtcagcttta cactgttttt atggctcttg gttatctaag
     2161 gaatctttta tcaaataaaa agtttcagtt gttttcgttt attagtgtaa tcgcttatat
     2221 tgctttatat caatggatca atattatagc gacagttatc ttatgggctg ggttatgtct
     2281 aagtatctat atcattgatc gaaacattga tatagcgata ttttatcctt cgattatttt
     2341 atttttctac atattcattg atggaacagc caaatatcta tataaaattt taggttggga
     2401 tatcagcgat ttttttacag ctgttttagg aacagggtgt ttgtggatcg ttactgtttt
     2461 tctaaaaaaa atggtcatac caaagattat tagggataaa acgactctaa aaatagcggc
     2521 catcgttgtt ttttttacga ttattgttta ttttattaac atttgtagtg atcgtttttc
     2581 tgttttagca agattcctag ttatcagtaa tgaattattt acggttcttt atggtgtaat
     2641 gtctaccata atttattgtg tactactttt tatcaagaaa aaagaattcg aaacacgaga
     2701 gcagcaaaaa gaaatgcgtc acctgattga atacagcgaa cagatcgaaa ataactatct
     2761 tgcattacga aaatttaaac acgattataa gaatattttg ttttcaatag aggattatat
     2821 tcaaacaaag gatattgaag ggctgcaaaa gtatttttat caaagtatca aagctactga
     2881 aacgattttt gaagaaaaca atctcccaat tgcttcgatt tcaaatctta aaattagaga
     2941 gataaaaagc attattttga gtaaattata cgtggctagc caaaagggtg tcgaagtaaa
     3001 cgtaatgatt ccaacaccca ttacggatat cgctatctca acagtcacac ttgttcgtat
     3061 gctagggatt atactggata atgcaattga agaatcagcc tttttagaaa aggggaaaat
     3121 tgaattgact attatggagg aagacaaaaa ttgttttttt gtcttaatga atcaatgtcg
     3181 ttcgagtatt cctaaattac atgaattgaa aaaagtgaat tttactacta aaaaaggaaa
     3241 tcaaggacta ggtctaacaa atctggacga gcttgttcat ttgaacgaga atgttttttt
     3301 agaaacaaaa attgaaaaca atcgctttat acaaatcata acgattagtg gaatgagggg
     3361 ctagtaatgt taaaaatatt tgtttgtgat gatgaaattg ctcatcgaaa taagcttact
     3421 cggatgatta caaattattt attgatgaaa gactatgatg ttcaattcca atttgctacg
     3481 gatgatccaa tgatgatctt gtcgtttatt tcaaaacata aaacagaagg aatctatttt
     3541 ttggatattg atctaaaaac ggatttaaat gggatcgagt tagcaaaaaa aatcagagat
     3601 tacgacccaa cagcaaaaat catttttgtg accacttata cagatttagt ctatttaact
     3661 tttttataca aagtagaagc attagattac attgtcaaag ataatatgga tacctttcaa
     3721 aagagagtga cggaatgtat tgacgtcgca attgaacgct atttgaatac aaatctggaa
     3781 actagacccc aaatttggat taagaatggt ccaattgatg taaagttata tgtcgatgag
     3841 atattttatt ttgaatctag tccaacaccc cataaattga tcgtctattt agataaccgc
     3901 agaattgagt atatagggaa gatcaaagaa gtagaaagta tgtatgaggt attttgtcgt
     3961 tgccatcaat cttatgtagt taatctgatg aatgtgaaag agatcaataa aaaagaacga
     4021 gtgatcacta tggccaatgg agcaaaatgt ttagtttcta ctcgtcattt aaaaaaagta
     4081 gtaaatcagt tcaagaaatt aaccagttgt tgattacatc tccaggtatt tcgcactttt
     4141 gatcaaataa cgttcacttt tgataaaacc aactatctta taaaaaaaca aatataatat
     4201 agatgaaaag gagcggatca aatgtcggaa gaatctatat ctttagaaga aaaagtttca
     4261 aaaacaatta gtaagcggat aggaagacaa ttacaattgt cagaaattga cttgacaaaa
     4321 atggaatatg gattagctgt tcttctggta attagttcaa agatattttt tatctactgt
     4381 ttagctggat gtcttcatat tattctgccg gttttaacta cccatttggc atttacacta
     4441 atgagaagaa caacaaaaag tttccatgca agagatagta atgtatgtac agccttaagt
     4501 ctatttttct ttgtcttatt accatatcta gttactactt ttgctgtaga tgtcacaaga
     4561 gtcaacttta tgatctgcag tctcatgatt tgcttgatct tgtggttcaa agggatagtg
     4621 atcgcacaaa aaccggcgaa aagttctttg ctaaatggaa agagccagat gatagtggtc
     4681 agtttgttcc ttactatttt tggtgggatt caatcaagta accgtattcg taccttgctg
     4741 ttgttaggca tgatgatctc aaccctttta atgtttgttc aaaaaagaaa ggatgaagaa
     4801 aatgttaaaa aaaattagct caagtaaatt aaaatatatg caaattttag caactgttgc
     4861 tattgccgtt gctacagaag ctataggtaa aggaacaatg tgtacaggaa ttatttatga
     4921 accaaaagta cctgaggcat tgaaacaaat cgaaaaataa acttggaaat cgaaatacta
     4981 gtggaataaa aagaattcat tatacgaata agtcttagga tcaaaaatag tagttgctat
     5041 tttgacccta agacttattt ttttcgataa ttttgtaaga gcaaaacaaa tataaaaaag
     5101 aaaaaagatt cccataatgt cgttgtccaa ctaaattctt ttgaaatccc aaaaaataaa
     5161 ttccctgcat tagaaacgat taaataaatt aaggtagtta ttagagcata aattattttc
     5221 tttaccatga tctcacaaca cccctttttt caaaaacatg gctaaagcgt tctatttctt
     5281 caaatgatct ttatttaagt ataattcatt taaaagatat ttggaaagaa attttatcta
     5341 tttttttaca gataagaaag aagaaagaga gaaggaaacg atcaatatta gaaaattatc
     5401 atagcaactg atagtttttt gtaataataa ctgagcatta tctttgcatt ttttaatgta
     5461 gactactatc ctttaaatat acccaataga aaaaaggcaa cacagtctat tggagggaaa
     5521 cgttatttat gtatgattgt catagaaagt gaaaaaaggc tattagtatt agcatgtcat
     5581 ggtagcgctt tctataaaat cttgtaaata acatgtgtga tcacggtcaa ttagaagtag
     5641 gaggtggaga atgaataaag ggtggaagat cgtcggtatt cttgcaagtt tattcttgct
     5701 aacggtgctt ttagggattg cgttgatcaa cgcaccgtat atgatgccag aacagttgga
     5761 acgttttcga ttttttgtca taacgaacta ctacgtgcaa caatatctat tttggacagc
     5821 tgtcgttttt gctatacttg tgcttatttt gttattagtt gttcttttct atccaaaatc
     5881 caaaggaacg tttgttttaa aacaaaaaga tggaaaactg actttagaca agaaagcaat
     5941 cgaagggctt gtgcgatcac aattacatga agaagaattt gttggttcac caaaagttcg
     6001 agtacgctca acaaaaaata aaatcaatgt ccatgtaaaa ggggatttaa aacgaacatc
     6061 ctctttaatt ggtaaaacag gagaattgat gcgtgacatt gaacaacaag tggcacgagt
     6121 acttggtact gaacaaaaca tcgctgtagc tgttacttat agtggatttg aagattatga
     6181 cagtaatgca cacaagcacg ctcgagtaga ataggagggg cccatcatga aagagatatt
     6241 ttcagcgtac aagctcccta ttatcttcgg gggacttggc ttactgttag ctgttttatg
     6301 gataacagtt ggatttttta aaacacttct aatattaatc atgactgcta taggtgcagg
     6361 cattggtttt tatttgaaag aaacaaaaat atttgatcaa ttttttaaat cttaggagga
     6421 actggacatg gaaaacaaag aaatcaaaaa cacaaataca atgacatctg agaataacca
     6481 tatttcacgt ggagaattaa cttacgaaga taaagtaatt caaaaaatca tcggttatgc
     6541 tttgaaagat gttgatggtt tattaacatt agatggcggt ttcttctcaa acattgctga
     6601 aaaattagtg aacacggata atgtcacagc aggaatcaat gcagaagttg gtaaaaaac
//
