LOCUS       NZ_VOEC01000028         6799 bp    DNA     linear   CON 11-APR-2024
DEFINITION  Enterococcus hirae strain DMW1-1 DMW1_1_contig_28, whole genome
            shotgun sequence.
ACCESSION   NZ_VOEC01000028
VERSION     NZ_VOEC01000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus hirae
  ORGANISM  Enterococcus hirae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Phylogenetic and comparative genomic of wild marine
            animal-associated enterococci from the southern coast of Brazil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2019) Basic Health Sciences Department, Federal
            University of Health Sciences of Porto Alegre, 245 Sarmento Leite
            St., Porto Alegre, RS 90050-170, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 8.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017356505.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 14:47:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 39 of 114
            Pseudo Genes (incomplete)         :: 71 of 114
            Pseudo Genes (internal stop)      :: 29 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:59:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6799
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOEC01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..181
                     /locus_tag="FQT05_RS12690"
                     /old_locus_tag="FQT05_12705"
     region          1..6799
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..6799
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6799
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1424:1799](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1425..1799
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     CDS             <3..181
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /locus_tag="FQT05_RS12690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12705"
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_207496273.1"
                     /transl_table=11
                     /translation="LDQQQQLTLQANQQIEKLQEQLQLTYTEKTLENESTTVTEEIIND
                     TPPPIEKKWWKLWK"
     gene            complement(831..1292)
                     /locus_tag="FQT05_RS12695"
                     /old_locus_tag="FQT05_12710"
     CDS             complement(831..1292)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /locus_tag="FQT05_RS12695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12710"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /transl_table=11
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     misc_feature    complement(1347..1363)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 19.42"
                     /tool="antismash"
     gene            1425..1799
                     /locus_tag="FQT05_RS12700"
                     /old_locus_tag="FQT05_12715"
     CDS             1425..1799
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /locus_tag="FQT05_RS12700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12715"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.4e-09,
                     bitscore: 28.1, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
     gene            1853..3979
                     /locus_tag="FQT05_RS12705"
                     /old_locus_tag="FQT05_12720"
     CDS             1853..3979
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /locus_tag="FQT05_RS12705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12720"
                     /product="hypothetical protein"
                     /protein_id="WP_207496276.1"
                     /transl_table=11
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFNFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
     gene            3976..4596
                     /locus_tag="FQT05_RS12710"
                     /old_locus_tag="FQT05_12725"
     CDS             3976..4596
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /locus_tag="FQT05_RS12710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12725"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /transl_table=11
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            4710..4847
                     /locus_tag="FQT05_RS12715"
     CDS             4710..4847
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /locus_tag="FQT05_RS12715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /transl_table=11
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
     misc_feature    5116..5135
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            5289..5669
                     /locus_tag="FQT05_RS12720"
                     /old_locus_tag="FQT05_12730"
     CDS             5289..5669
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /locus_tag="FQT05_RS12720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12730"
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_207496278.1"
                     /transl_table=11
                     /translation="MSEQNQNLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSASALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
     gene            5651..6589
                     /locus_tag="FQT05_RS12725"
                     /old_locus_tag="FQT05_12735"
     CDS             5651..6589
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /locus_tag="FQT05_RS12725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_12735"
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_207496280.1"
                     /transl_table=11
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKKKVRS
                     NKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVRREQRT
                     LTSEQPEIRPTRRNHEQHIERGTDHQQELSL"
     misc_feature    6645..6662
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
ORIGIN
        1 actcgaccag caacaacaac tcaccctaca agctaaccaa caaattgaaa aactgcagga
       61 acaactacaa ttgacttata cggaaaaaac tttggaaaat gaatcaacta ctgttactga
      121 agaaataata aacgatacac caccacctat tgaaaaaaag tggtggaaac tgtggaagta
      181 aaaagagaaa ttatttatgg aaaacaaaaa aagactgctc tttcaagcag tctataagta
      241 aaggtgttta tttgttatta agaacaatta gaaaggatac atgaaccata aaaattataa
      301 cactatttat tttaaaaata aaatcacaaa taaaattcta ttattagttt atggttgttt
      361 atttttaact aaatttgtta taatttttat ggttcagaag tttttagttt aattttgtgc
      421 aaacggacct ttttttatct aactcttcag aaatagctag aatgtaactt ctagctattt
      481 ctattttttt atcgtctaaa ttaaaaacaa tacataaaaa aagactgctc taacgagcag
      541 cccaaaatga gattatccta gtttagcaac ataaaccatt tattagatga aaatgaggta
      601 taaaaaaagc gtatcaaatc ctctcaaaaa taacaagtct ttaaaaagtg acagataaaa
      661 atgcacaaag aactaaaaaa gagaaatcgc ataaaatgag cattagaagc tagcaactag
      721 ctagaccaat tgaaaatcac aaagtctgcc cctgtactcg ttggtagagg ggctacttgg
      781 catactcgac attatgtaaa ctaagtgatt aaataactaa ctacttcata ttagcttaat
      841 aaaataatca aaataactag ggggaaaaac attgtataaa aaaatttttc attttttaaa
      901 taagaatagt aaatataaaa ataatttaag atatctatta ctattaatcc agatagagca
      961 aaccaagtaa atattccgtg tgtaaagcta aaaaaattca tttgaatcaa ttcaaataaa
     1021 cctaatatag taacaaatat attaaaaata atagctgtca ataccagtaa attatttttt
     1081 ttattttttg aattatcttc atttaaaata gaatttatgt tagtactata aaattcacaa
     1141 atttttttta gtgtctctat atctggtgtt gttttgttat tttcccactt tgaaatagtt
     1201 tgacgtgtaa cgttaaattt tcttgccaca tattcttgag tatacccttt tttcactctt
     1261 aattctctta gcttagatcc taaatcctcc attttttctc ctatgtaaac gattatttac
     1321 ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt tctttatttt ataaaacaca
     1381 ctaaaataaa ataattatag taaaaaagat aggagaaata agttatgaaa aaatttttat
     1441 gtctttctgc tttaatgggt gtcttattaa ctagtggagg aatagttagt gccacagaag
     1501 ctttaaatct agatgtaccc gaagaccata ccgccattta cggaggagga atggaagcta
     1561 cagaagaagg attttcttct agtaaattga gatatgctgc tggcggcggg gatttcaact
     1621 gtggggttaa tggatttaaa gtttatgcaa attactacca tgctagagct aaacatagtg
     1681 caacagctaa aaatggacga ggcggtcaag tacgatcagt tcagaaagct ggagtaagag
     1741 cttatgcaac atgcaatgcc acacttactg gaaatactgg ttggtggaat gtttattaaa
     1801 aaagagagtt taaactctct tttttattta catttgaaag gaaattcaaa atatgttaaa
     1861 aaaaataaac gctgttttat cactttttgt ctgcattaca atactatttt tttctactaa
     1921 tgttataaat ggtataaaga cttctgaagc gctatataaa aataccgctg aaattcatat
     1981 gcaatataaa gatgatatgg actttaacag ttatataaac gctttgtatg aaatatctac
     2041 acaagaaaat attaatattt ctcaatatag ttttactgct gttaaccaac taactattct
     2101 tgctacaaac cctaacgcta acaaacactg gaaattttct aaaaaaaata tattagcaag
     2161 aaacgattct aaagtccatt atactaataa caaaaattct aagtaccaat taaaacttcc
     2221 aaataatttt ttaaatataa atatttatcc tttttctcat gtggaaaata ttggattaag
     2281 tgaaatatta tatgtacaag gaaactcaaa caaattaatt cctgtactga aaaaatatgg
     2341 aacacctact atttccaata tcaatcaatc agatactttt caaattaata ctaatattgt
     2401 tcttgttatc tgctatctta gtatatttat tgtaattacc acaattatat ttgctttttc
     2461 aaaaatgaaa gaaatcactc taaaaaaaat gttaggctat aactcttttg atactataaa
     2521 agattctatt aaagagttaa acctcttatt ttgttctttg attataactt tcattttatc
     2581 tatttttttg agcatatatt acagtaacga aaattttttc ctatcatttc ttctatgtag
     2641 tatttttaat tttataatta gttttttatt tatcatagtt actctaagta tctatcattt
     2701 atctagtata aaaaattatc taaaaaattc acgaccactt aagttaaatc tttgtatatt
     2761 aaatatctgt ctattcttaa gcatgatcct tctgttaata gcttctacaa aagtaataaa
     2821 tatccataat gaagccgaaa ataattctct taaatattgg gaaagaacta ctaatttata
     2881 taaaacaaat attactaatc aattaaatag aaacaacacc gtagaagaaa ataactactt
     2941 gaaaaaagct agtaaatttg tttataaaat acagaagaat tataaaactt ttattatcgc
     3001 gccatacaat tacgcaacta tccaagagaa taataaagaa ttttttattg gacaaaaagt
     3061 ttatccaaat tttgaagact atgttagtca acctgctggt gcaggaatat gtgttgattt
     3121 aaactatcta aaatattaca atcctatttc ttttgaagaa tctactgact taaatttaat
     3181 aaactctgat ccactaacta cttacattct agtacctaaa aaatataaag aatatgcaaa
     3241 aatgattgaa aaaaattatt tagaagacat acagtttaga cttgaggact ctcctcctca
     3301 agctccttat aaaactccta atttgaaaaa tttaaaaata aaactaatat ttgttaataa
     3361 taaccaaaag tattttagtt ttaatacctt atatggaaag gcaaaagata actatacaat
     3421 aactgatccc atagttagag ttattaatcc agaaataaca gaatcattat tttggggaaa
     3481 tatattgact agtgatggtg gattattttt tgatcagaaa catacttcaa accaaaactt
     3541 ttttaatcaa attaatccgt atataaaaga atttaaccta gaaaatatca taaatttttc
     3601 ttattctgtt tttcaagaaa ctggtgaatt attatctcaa aaaaaagtaa ataaattaaa
     3661 tgcatatcta caatatcttt taatattagg attatttata ataattaatt ttcaaatgat
     3721 ttttgttagt tttactataa attcaaaaaa aatagcttta aaaaaaatat ttggatattc
     3781 accaataagt caagttttag atttatgttt actccctttt acaatagagt tatttgctac
     3841 tatgtgtttt caattgatat tttcaaaaaa cacttacttg tattttataa tttttatgat
     3901 ctttttaata aatctaatta cttccattat tatttataaa aaaaatagtt ttaaatattt
     3961 taaggagaat attttatgat attatcagtt gagaatttca ccctgtcaat taataaaaaa
     4021 aagatcaaac aaaattggaa cttttcaata tcagaaaaag attttattat agtaacagga
     4081 aaaagtggta gtggaaaaac tactctgtta aataatttaa gcctaataga taaacaatat
     4141 accggtaact tattctattt tgaaaagaaa gtttctaaac atgatataca acgtctacga
     4201 agaaatcata ttagctattt atttcaaaac tatgctcttc ttgaaaatca aacagtacaa
     4261 tacaatttcg aattagcaaa aaagtttaac agtaatattt ccaatgatca tatttataat
     4321 ttattaacag atttttcttt gccaaaagat atcttaaaag aaaaaatctt tctcttaagc
     4381 ggtggagaac aacaaagaat agcattaatt agaaatatgt taaaaccatt tgatatatta
     4441 tttgcagatg aacctacagg aaatttagat aaagaaaact caacttttat aatgaacttt
     4501 ctgaaaaatt taacagaaaa agaaaaaaaa gccgttgtac tagtaacaca tgacttaaat
     4561 gttctaaact atggaactaa tattatagaa ttataatttt taccagtgat gctagctgat
     4621 ttaaaactag caccactggt atttatttta tattccattt ctcctatccc aaaatttaaa
     4681 taatcactta ataaaaagga ggtacccaag tgattttatt gaaaaaagag tatgaaaaat
     4741 tttatgatag acttatttat tctttgcaat tgccaaaaaa attaaaccgt caattacaaa
     4801 agacttatga aactaaatca actgaagaat gggagagtta ttgctagatt tttggtagaa
     4861 gtaacaaaaa gtggacatat tatcataatg actcgccgaa agtctgataa aaaattggct
     4921 aaaaatattg ctgaatgttt aataaaacta tcagttttag ctaaatagtt agtagtttac
     4981 acaaaaaagc atttatgttt tttgttgcaa agcaacaatt tcatcttcgg tgaaataagc
     5041 gaaactttac aaaaagtggc tagcatttgc tagccttttt tgtattgtgg agcgtggctt
     5101 ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcactaaata
     5161 cgtaggtatt tggctagcaa atatagagct tgccaaaccc ttttagtgag ctttactcat
     5221 tgtacctttt taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg
     5281 tgggacttat gagcgaacaa aatcaaaatt tggctagcaa tcattctaaa aaatatacct
     5341 atcgatccga gccaaaacaa atcagttttc gagtgagcga atccgaattt gcaaagctga
     5401 agcaatcggc ttcagctttg caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa
     5461 acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt
     5521 tagccaaagc tggagggaat atcaatcaaa ttgccaagtg gtgcaataca caccaacatg
     5581 acgttgctcc tggcgacgcc caacgcttat ccgaaaacct aaaaatcatg caaaaggagc
     5641 tacaaaagat atggcaacag ttaaagtaag tcgaaccact tcatgtagcc gagcaattaa
     5701 ttacgcagaa ccacgagcaa ccgtcaaaac aggaataaac tgtgacattg attatgccaa
     5761 aagtgaaatg aaacaaatac gtatgcttta cggaaaagac gatcatgtac aagcacactt
     5821 attgattcag tcctttcgac caggagaaat taccgccgaa aaagccaacc agctaggaaa
     5881 agaatacgct gaaaaaatcg caccagaaca ccaaatcgcg atttatacgc acacggacaa
     5941 agaccatatc cacaatcaca ttgtcattaa ttcggtcaat ttagaaacag gaaaaaagtt
     6001 tcaagcacat ggtcaagcat tcctagataa gtgttacgat atcaacgacg aaatttgttt
     6061 gactcatggt ttaagtatca ctgaacgagg aaaaaaagaa gaaaaacgaa ctatgtctga
     6121 aatcaaacta aaagaaaaaa atgaacctgt ttggaaagac gagattcgct ttgccattga
     6181 tcaaaccatg aaaaatccaa aaaccagaac ctatgaccaa ttttgtgatt ccctcaaaat
     6241 atttgggatt cattgcttca atcgtgggaa aaattttact tacgaattaa tcaaaaaaaa
     6301 gaaaaaagta cgatccaaca aattaggaaa ggactacgaa aaggagacaa ttttacatga
     6361 gctggacaga cgagaacaaa cctattatcg agaacgagaa cgagcctatc atagacagca
     6421 agccgacgag cttcgaagag ctcgagaaag cattcggaaa attgctggag aagtacgaag
     6481 agaacaacga actcttacgt ctgaacaacc agaaattaga ccgactcgaa gaaaccatga
     6541 acaacacatt gaaagaggaa cagaccacca acaagaatta tcgctctaaa acgttaaaag
     6601 acatagaaaa cgaaataaaa agacaacgtg cttttcttgc gccttatacc caagaaagga
     6661 tacaaactac aattgattca caagtcaaaa aactccacca agcgctatta gaccagcgaa
     6721 ataaagaaca aaaaaaggcg ctcattcaac gatcaatgga ttttcttgcc cccttatgtt
     6781 gcttaggcct gacactgtt
//
