LOCUS       NZ_VOEC01000004        10216 bp    DNA     linear   CON 11-APR-2024
DEFINITION  Enterococcus hirae strain DMW1-1 DMW1_1_contig_4, whole genome
            shotgun sequence.
ACCESSION   NZ_VOEC01000004
VERSION     NZ_VOEC01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus hirae
  ORGANISM  Enterococcus hirae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 182963)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Phylogenetic and comparative genomic of wild marine
            animal-associated enterococci from the southern coast of Brazil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 182963)
  AUTHORS   Prichula,J., Frazzon,A.P.G., Seixas,A. and Gilmore,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2019) Basic Health Sciences Department, Federal
            University of Health Sciences of Porto Alegre, 245 Sarmento Leite
            St., Porto Alegre, RS 90050-170, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 8.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017356505.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 14:47:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 39 of 114
            Pseudo Genes (incomplete)         :: 71 of 114
            Pseudo Genes (internal stop)      :: 29 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:59:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10277
            Orig. end                         :: 20493
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOEC01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(40..>579)
                     /locus_tag="FQT05_RS02715"
                     /old_locus_tag="FQT05_02715"
                     /pseudo=""
     CDS             complement(40..>579)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358188.1"
                     /locus_tag="FQT05_RS02715"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="FQT05_02715"
                     /product="recombinase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="QRLGTEKIFIEKQSGATINQRPIFQEALEFVREQDIFIVETIDRL
                     GRNYDEIIDSVNCLKKKNVQLIITSLPIMAEAIGDLLLDKFIKDLII"
     gene            complement(<616..765)
                     /locus_tag="FQT05_RS02720"
                     /old_locus_tag="FQT05_02720"
                     /pseudo=""
     CDS             complement(<616..765)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /locus_tag="FQT05_RS02720"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_02720"
                     /product="ISL3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSQTQLIKDILNILDLNIHFEENCLTKEKYKGQICMIYRGILRYS
                     PEKCV"
     gene            1602..4484
                     /locus_tag="FQT05_RS02725"
                     /old_locus_tag="FQT05_02725"
     CDS             1602..4484
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340245.1"
                     /locus_tag="FQT05_RS02725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_02725"
                     /product="Cna B-type domain-containing protein"
                     /protein_id="WP_207494478.1"
                     /transl_table=11
                     /translation="MKKLNNLLVLTLFLLSNFSSLLPQFVYAEEGRDVSSNVTSLSVDP
                     KNVRDGENFTVRLEFDEKSIDIKPQDYIRVSWNSNGEIFGTGYNKNIDLLIQGKNVGTI
                     YVKNDNATIVFNENIRNLNDVEGWAQFEMLARNLTNTSQENTGDLTISSGNKNAEVSVT
                     KPASGNSSVFYYKTGDMLPEDTQHVRWFLNINNNKAYVEKEVYIIDEIQRGQRLDPDTF
                     EITTEMNGYSKNYRGTEGVAKFLTEYPGSTFNYSVDDNTITVILPANIVNLTTFRIAYK
                     TIITNDNQESFVNNSTAWFKEYNQPAVEGEAFNHSVKNINISGGITGTVKGEIKLVKNI
                     QGTDIGIPNVQFELRRVDGQLIQGKDSVTLKTNEQGVAVIKGLAVGEYAVKEISAPEWI
                     HFDPLNTPEIKFSISETDTKGIELNITNEKKKINVTATKKWEGGELPRPTIYFKLFRAI
                     STDNKLEEISNINLGEIKDGQDSFTWNDLDQYDDYGNEYKYSVKEVDQNGNDFVPNGYK
                     KIENGLTVTNQNIEQVNITGTKTWNDNEDQDGKRPKNITVNLLTNGKQVQSKEVSETDG
                     WKYEFTNLPKYENGQEITYTVTEKQVPEYNTEIKGFDITNSYTPGKTSVSVTKTWNDNN
                     NQDGLRPNSIKVQLYANGKAKGSPIELTEGNQWNHTWNDLAEKAKGQTIAYTVKEVDQV
                     PGYTNQVDESDLGNVKITNTHNPETTEVSGQKTWNDNEDQDGKRPKNITVNLLANGKQV
                     QSKEVSETDGWKYEFTNLPKYENGQEITYTVTENQVPEYNTEIKGFDITNSYIPGKTSV
                     SVTKTWNDNNNQDGLRPNSIKVQLYANGKEFGNVVKLSENNSWTTTWNNLSEKSGGQTV
                     TYTVKEVGKVDGYTSQVNEKNMGNIVITNTHKVDKKSTLINKENNKVNKDNIIKKFLPR
                     TGSHKSNIFILLGILVLAVSLILWIKKYKK"
     gene            complement(4730..4996)
                     /locus_tag="FQT05_RS02730"
                     /old_locus_tag="FQT05_02730"
     CDS             complement(4730..4996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307478.1"
                     /locus_tag="FQT05_RS02730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_02730"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_207494480.1"
                     /transl_table=11
                     /translation="MKSNKSINKVLELTETALATPEIKKDKNLCEILEKVKASAAKGEF
                     YYDYKKEFQPAISGFTIRNGFSTPKVLLELLVEVKTPKAWSGL"
     gene            complement(5001..5216)
                     /locus_tag="FQT05_RS02735"
                     /old_locus_tag="FQT05_02735"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_II"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341339.1"
                     /locus_tag="FQT05_RS02735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_02735"
                     /product="class II bacteriocin"
                     /protein_id="WP_242690719.1"
                     /sec_met_domain="Bacteriocin_II (E-value: 5.2e-18,
                     bitscore: 57.9, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKKLFSLALIGTFGLAVTNFGTKVDAATRSYGNVVYCNNSKCWV
                     NWEEAKENIAGIIISGWASGLAGMGH"
     misc_feature    5602..5617
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.82"
                     /tool="antismash"
     gene            complement(6142..6492)
                     /locus_tag="FQT05_RS02740"
                     /old_locus_tag="FQT05_02750"
     CDS             complement(6142..6492)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /locus_tag="FQT05_RS02740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FQT05_02750"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_207494482.1"
                     /transl_table=11
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKID
                     TAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDMSKKAGINSKYKANFTDKS
                     QFYSYSETMPQR"
     misc_feature    6545..6561
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.81"
                     /tool="antismash"
     misc_feature    complement(7175..7188)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.92"
                     /tool="antismash"
     gene            complement(7228..7527)
                     /locus_tag="FQT05_RS02745"
                     /old_locus_tag="FQT05_02755"
     CDS             complement(7228..7527)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FQT05_RS02745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FQT05_02755"
                     /product="hypothetical protein"
                     /protein_id="WP_207494484.1"
                     /transl_table=11
                     /translation="MKTKLYKLALVTLFSISSMGTSTIVKAESIPMKSGPQIQTRTRGS
                     IGKYGKRPIIYKSGLTSKQWNCYVNMAGSMILSSPSGSYVMLGTGLLSWNNACR"
     gene            7743..8147
                     /locus_tag="FQT05_RS02750"
                     /old_locus_tag="FQT05_02760"
     CDS             7743..8147
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FQT05_RS02750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FQT05_02760"
                     /product="thioredoxin family protein"
                     /protein_id="WP_207494486.1"
                     /transl_table=11
                     /translation="MKETSISVSEHQKKIPVNKKIAEAYKENTKNFIQINPENLKNIVK
                     EKRNKDIFVYFGRLSCPYCRNFVSLLTKDKLSNFDIYYIDTEDTDENLQIKKERTEYEI
                     DSVPSFIKITKNTFIKFNIEDGNFSNFFGI"
     gene            8743..8865
                     /locus_tag="FQT05_RS15215"
     CDS             8743..8865
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FQT05_RS15215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_278250496.1"
                     /transl_table=11
                     /translation="MKKTFFYLMIFMSCYRYCKEQKMVIIMGEGPTGIQTLDSL"
     misc_feature    8748..8759
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 18.98"
                     /tool="antismash"
     misc_feature    9377..9392
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    9553..9567
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.2"
                     /tool="antismash"
ORIGIN
        1 gttaaaagaa ttgctcgtta aaaataaata aaaaaatatt tatagatgta tatctttttt
       61 tattcgataa acagtctgtc tagtgatatt gtaatcttta gcaattttag aaatagcaac
      121 tccattattt aaatcctcaa caatgctttt acaaacaagg cgcggttgag gatcttttgt
      181 gtctgcacta tatagcttag ggcgtccact gtaaactccc ttcgctttag caatcttaat
      241 tccttgtgcc tggtgtcgtt taaacactgt acgttcctgc tctgcaatca tagctagaat
      301 ctaaatgatt aaatctttga tgaacttatc taggagaaga tctccgattg cttcagccat
      361 gattggtaaa ctagtgatta ttagttgtac atttttcttt ttgaggcaat tgactgagtc
      421 aatgatttcg tcataatttc tgcctaatcg atcgatggtt tcgacaataa aaatatcttg
      481 ttcacggaca aactctaaag cttcctggaa aatcggtcgt tgattaattg ttgctccaga
      541 ttgtttctcg ataaagattt tttctgtacc aagtctttgt gtcgtagcct ctagtgggac
      601 gacacagtgt caatgaacac atttttcagg tgaatagcga agtatcccac gataaatcat
      661 gcaaatttga cctttgtact tttctttcgt taagcaattt tcctcaaagt ggatatttag
      721 gtctagaata tttagaatat ctttgataag ctgagtctga gacatcttga atcctcctta
      781 tgaatggtta tgccaaatca attctaaagg aggattcaag gtgtcttttc tattttttaa
      841 tcaataaaaa aaatgtcagt ggaatccacc aacatcaaaa agtatagagc cgaaattaca
      901 cactagttgg taaggtgagg ataatcatgt agaaacttca gtgaatcttt catcacgaaa
      961 ttaggcacaa ctgtcgatcc ccaatatgaa atcgactcat acgcagagaa aggatttttg
     1021 gaaggttaag ttcactcgat gtaacaaatc aaactacgaa tattaccatg agaatataag
     1081 aacacgaaca ttttaagata aaaaaatgga gagacttatg aaaaatattc ttggaagcaa
     1141 gtacagttga aggagaaaat cctaaatcat caatatacat gttttactat caaaatctac
     1201 tgaaatctat gtatttaatt ataaaattta attggtgtta tatataagta caggaatatt
     1261 ttttaaatct aatattttta ggtatgccat tgatatatat aacgtacgaa aataaaatga
     1321 tggggtggtg aaataacttt cataatataa aatatattat taatattaag tataaaataa
     1381 tttaaaaata acagatttta atttcgtatt acataattat acctatatat atgttcagtt
     1441 ttatccaatg ttgactataa ataaaatatt ttgctatatt atacaaatca ggtataattt
     1501 gcttgtcttg ttaaaactgg agatagctat gtttgtttta tgttacgttt ttaacgtgat
     1561 tctttttagg tatgccaatt atatagttag gagaattaag aatgaaaaaa ttgaataatc
     1621 tactagtatt gactttattt ttgttatcca atttttcttc tcttcttccg caatttgtat
     1681 atgcagaaga gggtagagat gtcagtagta atgttacttc tttaagtgtt gatcctaaaa
     1741 atgtaagaga tggtgagaat tttactgtta gactagaatt tgacgaaaaa tctatagaca
     1801 ttaaaccaca ggattatatc agggtaagtt ggaatagtaa tggggaaatt tttggaacag
     1861 gatataataa aaacattgat ttacttattc aaggtaaaaa tgttggaaca atttacgtaa
     1921 aaaatgataa tgctacaatt gttttcaatg agaatatacg caatttgaat gatgtagagg
     1981 gttgggctca atttgagatg ctagcaagaa acttaactaa taccagtcaa gaaaatacag
     2041 gtgatttgac aataagtagt ggtaataaga atgcggaagt gtctgtaacc aaaccagctt
     2101 caggtaatag cagtgtattt tattataaaa ccggtgatat gcttccagaa gatacacaac
     2161 atgttcgttg gtttttgaat attaataata ataaagcata tgtagaaaaa gaggtatata
     2221 taatagatga aattcaacga gggcaaagac ttgatccaga tacttttgaa atcacaacag
     2281 aaatgaatgg ttattcaaag aattacagag gcacagaagg agttgctaaa tttttaacgg
     2341 aatatccagg atcaacattc aactattcag tagatgataa tactattact gtcatcttac
     2401 ctgccaatat agtaaatcta acaactttta gaattgcata taagacgatt attactaatg
     2461 ataatcaaga gtcctttgtt aataattcta cagcttggtt taaagagtac aatcaaccag
     2521 ccgttgaagg cgaagctttt aatcattcag tgaaaaatat aaatatttca ggcggaatta
     2581 ctggaacagt caaaggcgaa ataaaacttg tgaaaaatat tcaagggaca gatattggga
     2641 ttccaaatgt tcaatttgag ttaagaagag tagatgggca gcttattcaa ggaaaagata
     2701 gtgtcacttt aaaaacaaat gaacaaggag ttgcagtaat taaaggttta gcagtaggtg
     2761 aatatgctgt aaaagaaata agtgcaccag aatggattca tttcgatcca ttaaatacac
     2821 ctgaaataaa attttcgatt agtgagacag atactaaagg gatcgaatta aatataacta
     2881 atgaaaagaa aaaaataaat gttacagcaa cgaaaaaatg ggaaggtggg gaattacctc
     2941 gaccaactat ttattttaaa ttattccgcg caatatcgac ggataataaa ctagaagaga
     3001 tttcaaacat aaatctagga gaaattaaag atggacaaga ttcatttaca tggaatgatt
     3061 tagatcaata tgatgattat ggtaatgaat ataaatatag tgtcaaagaa gttgatcaga
     3121 acggaaatga ttttgttccg aatggatata aaaaaataga aaatgggctt acagtaacta
     3181 atcaaaatat tgaacaagtt aatatcacag gaacaaaaac atggaatgac aatgaagacc
     3241 aagatggaaa acgaccaaaa aatattacgg taaacttact gacaaatggg aagcaagtac
     3301 aaagcaaaga agtaagtgaa accgacggtt ggaagtatga gtttactaac ttaccaaaat
     3361 acgaaaatgg acaagaaatc acctatacgg tgacggaaaa gcaagtgcca gagtacaata
     3421 cagaaatcaa aggattcgat atcaccaaca gttacacccc tggaaaaacc agtgtaagtg
     3481 taacaaaaac atggaacgat aataataacc aagacggtct acgtccaaat agtatcaaag
     3541 tccaattgta tgccaatgga aaagccaaag gatcaccaat tgagttgaca gaaggaaatc
     3601 aatggaacca tacatggaat gatctagccg aaaaagcgaa gggacaaacc attgcctata
     3661 cagtaaaaga agtagaccaa gtgccaggtt acacgaatca agtagacgag agtgatttag
     3721 gaaatgtgaa aattacgaac acgcataatc cggaaacgac agaagtatct ggtcaaaaaa
     3781 catggaatga caatgaagat caagatggaa aacgaccaaa aaatattacg gtgaacttac
     3841 tggcaaatgg gaagcaagta caaagcaaag aagtaagtga aaccgacggt tggaagtatg
     3901 agtttactaa cttaccaaaa tacgaaaatg gacaagaaat tacctatacg gtgacggaaa
     3961 accaagtgcc agagtacaat acagaaatca aaggattcga tatcaccaac agttacatcc
     4021 ctggaaaaac cagtgtaagt gtaacaaaaa catggaacga taataataac caagacggtc
     4081 tacgtccaaa tagtatcaaa gtccaattgt atgccaatgg aaaagaattt ggaaacgttg
     4141 tgaagttgag tgaaaataat agttggacta caacgtggaa taacctatcg gaaaaatctg
     4201 gaggtcaaac agttacgtat acagtaaaag aagttggaaa agtagatggc tacacgagcc
     4261 aagtaaatga gaaaaatatg gggaatatcg tgattacaaa cactcataag gttgataaaa
     4321 aaagcacatt gatcaataaa gagaataata aagtcaataa ggataatatt attaaaaaat
     4381 tcctacctag aacaggtagt cataagtcaa atatattcat acttcttggt atattagtat
     4441 tagcagtcag cttaattttg tggattaaga aatataaaaa gtgagatctt tataattatt
     4501 ggaattggat cgataggttt atttaaaaac atcaaagcta gtaaaatttt cggctttttg
     4561 tcaaacaata ttggtgaaga gaaaccctag aatgattggt gaacagaaag aactattttt
     4621 ctttctgttc cttccaaata tattgtaaca ttttaatttc tatattccat atgttcctta
     4681 aaactcaatt agatactttt ataaaaaata gaatggcact tatcataact caaagtcccg
     4741 accatgcttt gggagttttt acttcaacaa gtaactccaa taaaaccttc ggggtggaaa
     4801 agccgtttct aatagtgaat ccactaattg caggttgaaa ttctttcttg taatcataat
     4861 aaaattcacc tttagcagca ctagctttta ctttttctaa aatttcacat agatttttat
     4921 cttttttaat ttccggggtg gctaatgctg tttcagttaa ttctagaact ttgttgatag
     4981 atttattact tttcatagta ttaatgtccc atacctgcca aaccagaagc ccagccacta
     5041 ataatgattc ctgcaatatt ctctttagct tcttcccagt taacccagca tttactatta
     5101 ttacaataaa caacattacc atatgaacgc gtagctgcat caacttttgt accaaaattt
     5161 gtcacagcta acccaaatgt tccaataaga gctaaactaa ataatttttt tctcataaat
     5221 caataccttc tttttctaaa ataattataa atgattttta gaaaaatttg ataattatat
     5281 cataaacagt tgttttgaaa gcataatggc aataaaaaat cattgttaaa actaatctca
     5341 tttgtattct tctgggaatt aattcattcc ctcgaaaatc gagtgtcaca caataacgcg
     5401 cataatttcc tacttttctc aaatattttg ctacttgatt ttcatattct tctattcctg
     5461 gagtataccc tattgagatc taattttata tctatctgaa cttaattaaa tgaactattg
     5521 aaagaacgct gaaatgagga attaaaacag gatcaaaacc ttaaaagttg ttggcccttc
     5581 tataaagcta tagaatattt ttcgaatttt tgtgtgacaa atactgagaa aaatttttgc
     5641 catttaaata gaaaagtgac aactagattt ttcaatgatc cgtcagtgtc tatttagggt
     5701 acactcaaat ttaacactac aaaatacttg aatgcaaaat tcatttgttt tatcgaagaa
     5761 gataattggt cgattcaatc gcagaaagaa gttgccactt ttgaaagagg aaaagctatt
     5821 cttaagcagt ggatattaaa taacttagaa tttgtaaact tagaccaatt agtactaaga
     5881 tatattgatc cagcaaagca gaaatttgga ctatccatta tttcaggtaa gagaatctat
     5941 gtagaatata gaaaacaata tggtatttag tgttcataca acgattctaa ggtcattttg
     6001 aattaactag ttgtatagtt ttgtcaaaca agatgaatga gaatctttta tagtagtgaa
     6061 cctagttgaa caaaaaaagt tagacatttt tgtctaactt ttctttatca attttttata
     6121 tttatagtag tgtactatat tttatcgttg aggcattgtt tctgaataac tataaaattg
     6181 cgatttatct gtaaagtttg ctttatattt actattaatt ccagcttttt tagacatatc
     6241 ccctaattct attaataatt tttcatcatt atcaataaag cgtattttat tatcaaaacc
     6301 cacgatgtaa atataattaa ctaatttatt tacaagaatt tcaggatatt ttgctgtatc
     6361 aattttttta tatacctgaa ataaaacatc agtaatatct aataatgctt tcgtctgatt
     6421 aggtcgtgtc attaaactat tgtataattc atgtatgact ttttttgtgt gatcaatgtt
     6481 tattgaagtc attaattttt tccttttcga atttttttag attgcttgta atattaaata
     6541 actttagtaa ttttattata taacatttta ttatataaca atgattgacg tcataaaaag
     6601 tagctttgaa ttcataaacg tttatataac gattaataga aaacaatata gtatttagtg
     6661 ttcatacaac gattctaaga tcattttgag ttaatcagcc ctatagttgg ttaaacaagg
     6721 agaataagac gatctctaaa ttgtcattac taagaatttt tgccatttaa aaagaaaagt
     6781 tattcactta ttttgagatt aaaaaatatg tttaaatgaa aactgagata taagatttga
     6841 tgagcaatca aaatcttata tctgtcattt attactgaaa tatttttccg tttgaaaaga
     6901 ggtatatcct caattactta tttttcataa tgttgtcttg cagtttaaaa atcccattta
     6961 taataagaaa aagcaggaaa tgtttttata taaacgtttt ttaatgatgt tctcttattt
     7021 taaaatatat atattgccca attgttgtta ttataataaa aagtataacc atcactaaat
     7081 atcgtgtgaa agttgttaaa ttaaatgaac ccaagctaaa gtaacttata agttgactaa
     7141 caaaaggttg aataataaca ataaacaggt taaatttttt tttaaaaaca tatttagtat
     7201 tcatgacaac atcttctttc acgtagctta cctacaagca ttattccaag ataataatcc
     7261 tgtgcccaac attacataag aaccagaagg agaagataaa atcattgaac ctgccatgtt
     7321 tacataacaa ttccactgct ttgatgtcaa tccactttta taaataattg gacgtttacc
     7381 atattttcct atagatccgc gagtgcgtgt ttgaatttga ggaccacttt tcattggtat
     7441 actttcagct ttaacaatag tagatgtgcc catactagaa atactgaaca aagtcactag
     7501 tgctagttta tataatttcg ttttcatttt aattcctcca atctatttga tataattaaa
     7561 tataactagt tatattacaa ttttatttta tcaaataaat aattaaagcg ctttctaaaa
     7621 ttgaaaatgg agtggtttag taataaaaaa aattaaataa tttttttagt ttaaatatac
     7681 ttttatttat atttttaatt ctacaagtga gttgttctaa aaagggttct acacatcaac
     7741 aaatgaaaga aacttctatt tcagtttcag agcatcagaa aaaaattccc gtaaataaaa
     7801 aaattgctga agcatataaa gaaaatacta aaaattttat tcaaattaat cctgagaatt
     7861 taaaaaatat cgttaaagaa aaaagaaata aggatatatt tgtatatttt ggacgactaa
     7921 gctgtccgta ttgtagaaat tttgtttcat tattgactaa agataaactg tctaattttg
     7981 atatttatta tattgataca gaagacacag acgaaaactt acaaataaaa aaggagagaa
     8041 cagaatatga aattgactca gttcctagct ttataaaaat aactaaaaat acattcataa
     8101 aatttaatat tgaagatgga aactttagta atttttttgg aatttgaaaa acaataaaat
     8161 tataaaatga aattgttaaa ccttaaattt aatgaagtta acaattgaaa tttataatat
     8221 gttataaaaa ttatttctaa agttagaatc aatggaaata cttcaaacat gtatatttga
     8281 ttgatttttt aagtgaatta ttttttgttt tggatataaa taatatgaaa gaagggaact
     8341 tttaatgtct aaactgaatt ggttagtagt caaaaaaatt attttgatta ttttaacttt
     8401 tactgcaggg atggtatttg cagacatagt tttttcaacc attttgaact gaattgatta
     8461 tacaaataaa ggctctttgt caaatgatgt tggtaaagag aaatcttaga attatttgga
     8521 cagaaagaag aaaatttcgg ctctttgtca ataagaactg atgagtggtg taaaaaaaaa
     8581 tctgagacag aatgaggttc attctgtctc agattttttt gttatagagc cattttcttt
     8641 ttgaagcgtt gtcctactct ctatacagaa aatcattcca catcatgaaa agaaccgtac
     8701 ctactgccgt acaagaagct aaaatgaata gaggtaatga agatgaagaa aacgtttttc
     8761 tatctgatga tttttatgag ttgctatcgc tactgcaaag agcagaaaat ggtcattatc
     8821 atgggtgagg gtcctacagg gattcaaacg ttagatagcc tatgattttt cttgcttcta
     8881 ggtatagaga aagttgtttt tacccactca tctgtgtttg aaataattca tctaatcaaa
     8941 ctgagccaag aaaataccat aatggtaatc atctaatttt aaaaaaatag ggatctatcc
     9001 attgcttttt tgaatacctt aatagtttaa aatttctccc gctctataca attatgaatt
     9061 aaatgaattg gactttagtc taatgttttt ttacattaat caatactata attaatatag
     9121 tattgtggat ttaagaaagt gaggaaagag aaaatataga tattagagtt gattatctcc
     9181 aaattttttt tggtacatat tctattttta tttctagcct attaaagtca aatttgcttg
     9241 ttggagggat tatggatagt aagaatcaaa aactatttat tgctcagttt attgcaacga
     9301 tttgtgttgt tgttattcat tgtgggacca ttactagtca cccgatagtg catttttttc
     9361 taaaaagtat ggtttgtcgt attgctgttc cattcttttt tgtaaataat gcatatttct
     9421 ttcggataaa tagtaagatg gaaggctatt cgaagatgtg gctaagaaga atagtacgtc
     9481 tatatactat tgtgtttttg ctgtacattc cgtttggtgt tcaactgtta caagatacag
     9541 ttgatattca gtggaattta tggtcaatat tattggcatt ggtagttggt ttcctatata
     9601 ctggaagctt ctatcactta tggtattttc ctgctttgat aagttcatta gctatagtta
     9661 aatatatgtt tcaaagattt agatataaat gggtttttct ggtttgtttt ttattttttt
     9721 ctgttggttc ccttgaaacg tattccttat ttatatcaaa tccaatggtc atatcaatta
     9781 tggaaaaata ctttaatatt ttttttacta caagaaatgg gttattcttt agtccactct
     9841 ttatttccat aggatttgtt ttggctgata atgtacataa tttagaaaag catcgaaagt
     9901 tattaatata tggactagtc gctgcgtgtt ttttaggagg agtcgaaggg ataatcacat
     9961 tttataatca aggaatcgat aagaatttta tgtatttcac aatccctttt atttttagtt
    10021 tgtatggact gctggttatt tctaaaagta aaatcaaaag ttttgaaaat ttaaatttct
    10081 atagtaagaa tatattcttg tttcatatga tgcctattca aatctttaat atgaggcatg
    10141 gcgaaataac acctataaat ggattattcc gcttgatgtt aggggtgttt gttccaataa
    10201 gcattgtttt atttta
//
