LOCUS       NZ_CP070896            20888 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Lacrimispora xylanisolvens strain ASCUSBR21 chromosome, complete
            genome.
ACCESSION   NZ_CP070896
VERSION     NZ_CP070896.1
KEYWORDS    RefSeq.
SOURCE      Lacrimispora xylanisolvens
  ORGANISM  Lacrimispora xylanisolvens
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Lacrimispora.
REFERENCE   1  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Hungatella xylanolytica ASCUSBR21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) R&D, Ascus Biosciences, 6450 Lusk Blvd E209,
            San Diego, CA 92121, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAY-2019
            Assembly Method                   :: Canu v. 2.1.1
            Assembly Name :: Hungatella xylanolytica ASCUSBR21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017084465.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,443
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 686
            CDSs (without protein)            :: 686
            Pseudo Genes (ambiguous residues) :: 0 of 686
            Pseudo Genes (frameshifted)       :: 599 of 686
            Pseudo Genes (incomplete)         :: 95 of 686
            Pseudo Genes (internal stop)      :: 24 of 686
            Pseudo Genes (multiple problems)  :: 30 of 686
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:11:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4052653
            Orig. end                         :: 4073541
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="9"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20888
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10888](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10888
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            439..1452
                     /gene="spoIIIAA"
                     /locus_tag="JWH23_RS22435"
     CDS             439..1452
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401464.1"
                     /locus_tag="JWH23_RS22435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AA"
                     /protein_id="WP_349772590.1"
                     /transl_table=11
                     /translation="MRVERKDELINLFSKNVREILNKVALNFDEVQEIRLRVAAPLMLV
                     YRNEEYYLTGSGNISKKLEEAYHITKNELKETMEYMSNYSLYAFEEELKQGFLTVQGGH
                     RIGIAGKTILDESGIKTMKYISFINIRLSHQVKGCASPILPFVYKNQREVCHTLIISPP
                     RCGKTTLLRDLIRQISDGSREFEGMNVGVVDERSEIGACYQGAPQNDLGIRTDILDCCP
                     KAKGMMMLIRTMSPRVIAVDEVGSREDLDAIEYVMNCGCKLIATVHGSSIEDLKQKPVL
                     RSLLEERIFERYIVLNNLGRIGNIDQIFDSRGTQLYQANIFGTGVRPDRQESRAYG"
     gene            1445..1975
                     /locus_tag="JWH23_RS22440"
     CDS             1445..1975
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601072.1"
                     /locus_tag="JWH23_RS22440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AB"
                     /protein_id="WP_277406280.1"
                     /transl_table=11
                     /translation="MGNIWLRLAGAALVIGSCSGLGFYMASQWNEHLKTVEHLRKMIFL
                     LKGEIVYANSPLAEAFERTGRKAGGQMGDLFLKVSDRLMGQRGESFYGIWQEEIDGLPK
                     EVCLSSEDKQNLKGLGEHLGYLDTGMQERTILLYLEQLDLSIDYLRNHKQEKSRLYTSL
                     GIMGGLFLTIVMY"
     gene            1986..2180
                     /gene="spoIIIAC"
                     /locus_tag="JWH23_RS22445"
     CDS             1986..2180
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779097.1"
                     /locus_tag="JWH23_RS22445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AC"
                     /protein_id="WP_013273849.1"
                     /transl_table=11
                     /translation="MGVNLIFKIAAVGILVSVICQVLKHSGREEQAFLTSLAGLILVLF
                     WLVPYIYQLFESIKNLFAL"
     gene            2189..2575
                     /gene="spoIIIAD"
                     /locus_tag="JWH23_RS22450"
     CDS             2189..2575
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601070.1"
                     /locus_tag="JWH23_RS22450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AD"
                     /protein_id="WP_170820156.1"
                     /transl_table=11
                     /translation="MTVVSIAVVGIVAVLLAVSLKGMKGEYGTFLVLAAGFFIFFYGVG
                     KLSTILDTMKEIQSYIKINSVYLSTLVKMIGITYIAEFASGICKDAGYGAVGTQIEIFG
                     KLSVLAVSMPILLALIETLQVFLS"
     misc_feature    complement(2202..2216)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.55"
                     /tool="antismash"
     misc_feature    complement(2703..2716)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 22.87"
                     /tool="antismash"
     gene            2771..3763
                     /locus_tag="JWH23_RS22455"
     CDS             2771..3763
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021088.4"
                     /locus_tag="JWH23_RS22455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AE"
                     /protein_id="WP_277409949.1"
                     /transl_table=11
                     /translation="MNLSFQDLMKSLMDGKLNDVLSQTGKALKKMLMGEIESSGHMMGQ
                     ILVLGIIGAVFSNFSSVFTGSQISETGFFVTYLLLFTYLAASFFTSITIVSGVVNQILG
                     FMRVLMPAYFLAAAFAGSSATAVASYEFTLVILSASQWLLGTVLLSLIRVYALLVMAGH
                     IAKEDMLSKLTELLEQVVSWSLKTLVGLVLGFQMIQSMVLPYVDSLKTGAVQKLVGAIP
                     GIGQGVSSVAQMVLGSGVLIKNTIGAAGILILIILTIVPILKLVILMLFYQCVAALLQP
                     VCDKRIVSCISDMAKGHKMLLSVAISCMILFIVTIALVCASTNVTYYSG"
     gene            3769..4287
                     /locus_tag="JWH23_RS22460"
     CDS             3769..4287
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021121.4"
                     /locus_tag="JWH23_RS22460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AF"
                     /protein_id="WP_277406281.1"
                     /transl_table=11
                     /translation="MDQLYEWIRNITYYLIFITVVENLLPNKKYEKYVKFFAGMVLILL
                     VLKPVTGGLRLDDKLAYSFEAISLKKEAGELTGKVSKMEETRLKRMISGYEKAVGTDLE
                     TMAQTAGYSCKETSAEINSDQSSDRFGHVVKVSLVLTSGKEEMQAEEAASVGSKMDPVK
                     KVEEIKTLK"
     gene            4420..5031
                     /locus_tag="JWH23_RS22465"
     CDS             4420..5031
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601067.1"
                     /locus_tag="JWH23_RS22465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AG"
                     /protein_id="WP_349772591.1"
                     /transl_table=11
                     /translation="MKFKWKMGKDKLLILLAVGMMILILTFPSGPGIAKKVEKTAQKDN
                     NKQSTLQQGIVTQPADGDAAASVSADRTYEEELEARVKKILKTVDGVGQVDVLIVVKSS
                     GEKVLRVDKDNSNSSTEENDTSGGSRKITSAELKESTILTGQGENTAPIVEKEIRPEIE
                     GIIISAQGGGSPTVKAEISSAMEALFNLPPHKIKVLKRVE"
     gene            5047..5901
                     /locus_tag="JWH23_RS22470"
     CDS             5047..5901
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771937.1"
                     /locus_tag="JWH23_RS22470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIIAH-like family protein"
                     /protein_id="WP_277406283.1"
                     /transl_table=11
                     /translation="MKSWKTSNEPNVPKTPKKMDMKKLFRRNQIIITTLAVMIAAAGYL
                     NYAGKQDAASGTDVYEAGMTDISEEDILAENQALNGSDSSKDIASLDQDAADIDKLAAA
                     DSAGSADKTAAGSTQVAAGNETAQSLAAGETQAAASLAEGETQAADTGLDNPGEAVLTS
                     GMNVADYISSVQLNREQVRARNKETLMNLINNPNIEEAAKQQAIQQMVDMTAIAEKENA
                     AETLLLAKGFADPVVSISNGKVDVVINAASISDPQRAQIEDIVKRTEVGAESIVITLMK
                     LEE"
     gene            5978..6364
                     /locus_tag="JWH23_RS22475"
     CDS             5978..6364
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601061.1"
                     /locus_tag="JWH23_RS22475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="WP_349772592.1"
                     /transl_table=11
                     /translation="MAELENRNTHKVYEKDKIGEVQIADDVVAIIAGLAATEVDGVDSM
                     AGNITNELVAKLGMKNLSKGVKVELTEEHVSVDLSLNIKYGYSIPNVSEKVQEKVQNAI
                     ENMTGLSVLDVNIRIAGVALDENK"
     gene            6470..7153
                     /locus_tag="JWH23_RS22480"
     CDS             6470..7153
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS22480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="cache domain-containing protein"
                     /protein_id="WP_277406284.1"
                     /transl_table=11
                     /translation="MDDIVNRKILKDRCFLNMMNRRNGGHYPPGLLILASLVISVIIGI
                     CVLNFYYFHEVEQSLFQQTHRDLKQKNDKALNYIETMIQTKFEWLELFAFYCDLPNGSG
                     NEQWWQQVQDYEKEDSRFGVADTSGTLYYGNRETQDISSRDYYKKALDGEKNISGLLKE
                     SFKGQDSIILTVPIIRDGVVKGAACLELSSDKLKQYLSDTDLSRYGANMIFQKRWRDSD
                     FLYGM"
     gene            7176..7949
                     /locus_tag="JWH23_RS22485"
     CDS             7176..7949
                     /EC_number="2.7.7.65"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /locus_tag="JWH23_RS22485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diguanylate cyclase"
                     /protein_id="WP_277406285.1"
                     /transl_table=11
                     /translation="MLKTRKFGPGQTFSEMEAGISQGLSGYITYYENDYRRLLYYQPMG
                     INDWYMATFVETEGYENTFHKIKSLSAKFIAGSTFLLFCGVILILLIVHTRKKEEKRLQ
                     KDYLTGVYTRETARKLVEHGLKAGGKNRFYACMFLDIDDFKKINDTFGHHKGDLVLVQA
                     GQILSACTRQEDVIVRFGGDEFCIWLYGMRGRKQPEAIAQRIINAFHVSGKVHASIGIT
                     LIGENETEYDAILKRADQALYEAKGKGKNQYAVSN"
     gene            8053..8496
                     /gene="nusB"
                     /locus_tag="JWH23_RS22490"
     CDS             8053..8496
                     /GO_process="GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /gene="nusB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601007.1"
                     /locus_tag="JWH23_RS22490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcription antitermination factor NusB"
                     /protein_id="WP_277406286.1"
                     /transl_table=11
                     /translation="MTRSKMREHCFKMLFCADFHPAEEKLWQINQYFEEPKEDDLNTEG
                     VEEIIHDVDMSEENSAFLKEKTEAIMNKIPELDEKINEVAEGWKTKRMGRAELTILRLA
                     LYEILFDDEVPEKVAINEAVELGKKYGGNEAPAFINGVLAKLV"
     gene            8499..9407
                     /gene="xseA"
                     /locus_tag="JWH23_RS22495"
     CDS             8499..9407
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /locus_tag="JWH23_RS22495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_349772593.1"
                     /transl_table=11
                     /translation="MAGVYSVTQINGYIKNMFAQDFALNRISIKGEVSNCKYHTSGHIY
                     FTLKDKASSISAVMFAGSGKGLSFHLEDGQQIIAKGSVEVYERDGRYQLYAQEITKEGM
                     GDLFERFIKLRNELEEMGMFSPEYKKEIPGYARRMGVVTAPTGAAIRDIMNISARRNPF
                     VQLYLYPALVQGSGAAESIVKGIETLDQMGLDVLIVGRGGGSIEDLWAFNEEAVARAIF
                     NCSVPVISAVGHETDVTIADYVADMRAPTPSAAAELAVFDYKQFKEQTESIRYTLKNHD
                     GAKAGAVSFLCEPIWIKVKVL"
     gene            9373..9786
                     /locus_tag="JWH23_RS32475"
     CDS             9373..9786
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /locus_tag="JWH23_RS32475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_349772594.1"
                     /transl_table=11
                     /translation="MNQYGLKLKYFDPKRSIREQRQRLHDLEEKLKSQLLIQIEAERRQ
                     LDESAVKMSQLMEKQTLRDRHRLELLAGRLHACSPLAKIGGGYGFITDSGNHRIDSVMQ
                     VKAGDALRLKLRDGNIHTLVSEVEPLTVSEAEK"
     gene            9801..9995
                     /gene="xseB"
                     /locus_tag="JWH23_RS22500"
     CDS             9801..9995
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553747.1"
                     /locus_tag="JWH23_RS22500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_349772595.1"
                     /transl_table=11
                     /translation="MAVKKERTIEETLKDLEELIKKLESGESSLEESFEYYETGMKLVK
                     SCNDKIDKVEKDNCTGGKW"
     gene            10001..10888
                     /locus_tag="JWH23_RS22505"
     CDS             10001..10888
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397627.1"
                     /locus_tag="JWH23_RS22505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_277409951.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 6.9e-54, bitscore:
                     181.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFEDELLKRTKDVWEIVESFLPAVEGHQSTVLEAMDYSIRAGGK
                     RLRPMLMRETYCLFGGMGKEIEPFMAAIEMIHTSSLIHDDLPCMDNDTLRRGLPTAWVK
                     FGYDMAVLAGDGLLIYSMETAAKALDFSDRPDRVARAMGILAQKTGIFGMIGGQTVDVE
                     LAGKPIPGEKLDFIYRLKTGALLEASMMIGALLGGASEDELKAVEEMASKIGLAFQIQD
                     DILDVTSNAEVLGKPVFSDEKNNKTTYVTLEGMEQAKMAVERISAEAVACLQKLPGENR
                     FLEELIRMLVNREK"
     gene            10903..12804
                     /gene="dxs"
                     /locus_tag="JWH23_RS22510"
                     /pseudo=""
     CDS             10903..12804
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="dxs"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601003.1"
                     /locus_tag="JWH23_RS22510"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /pseudo=""
                     /sec_met_domain="DXP_synthase_N (E-value: 4.4e-77,
                     bitscore: 257.5, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMLELINGPDDIKKFSKQELTILSQEIRDFLIEKISTTGGHLASN
                     LGVVELTMAIYLAFDLPMDKIVWDVGHQSYTHKILSGRRMEFDELRQYGGMSGFPKRKE
                     SPCDAFDTGHSSTSISAGLGLAQARDVLGEGHFVVSVIGDGSLTGGMAYEALNNAAQMK
                     KTSLLFSMTIICPYPKM"
     gene            12806..13621
                     /locus_tag="JWH23_RS22515"
     CDS             12806..13621
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601002.1"
                     /locus_tag="JWH23_RS22515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_277406287.1"
                     /transl_table=11
                     /translation="MKERLDVLLVKQGHAESREKAKAIIMSGIVFVNGDKEDKAGSTFE
                     ETALIEVRGSTLKYVSRGGLKLEKAMTHFNVTLEGKVCMDVGSSTGGFTDCMLQNGGVK
                     VYAVDVGHGQLAWKLRNDERVVCMEKTNIRYVTPEDIKDRIEFSSIDVSFISLTKVLLP
                     VKNLLSEDGEIVCLIKPQFEAGREKVGKKGVVRDKSVHLEVIQSVISYGISIGFEILHL
                     EFSPIKGPEGNIEYLLHLKNHPAGESFPEYSVNPEKVVEEAHKDLSEGK"
     gene            13632..14456
                     /locus_tag="JWH23_RS22520"
     CDS             13632..14456
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553743.1"
                     /locus_tag="JWH23_RS22520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(+)/NADH kinase"
                     /protein_id="WP_349772596.1"
                     /transl_table=11
                     /translation="MKHFYVIMNPDKKGARETAEQIREFLTARGAVCLIGRGEKVEWQA
                     GKHYTDSAMVPKETECVITLGGDGTLIQAARDLSGRNLPILGINRGTLGYLTQVSRTED
                     IESALSALFFDDYKLEERMMLSGSAFRDGKEIYRDIALNEIVITRGEYLKMLQFKVYVN
                     GEFLTEYRADGLIAATPTGSTAYNLSAGGPIIVPDSKMLVLTPICSHALNARSIVLSAE
                     DKIRIEIIGQEGISQAAVFDGDSAVSLLPGDWIDIERAQIKTILVKLKNLSF"
     gene            14501..14950
                     /gene="argR"
                     /locus_tag="JWH23_RS22525"
     CDS             14501..14950
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="argR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397621.1"
                     /locus_tag="JWH23_RS22525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="arginine repressor"
                     /protein_id="WP_104435066.1"
                     /transl_table=11
                     /translation="MKLERHSKIVELIGKYEIETQEELADYLNQAGFAVTQATVSRDIR
                     ELKLTKVQSESGKQRYMVLQNQGSFSDKYIRILRDGYLSMDMAQNILVIKTVSGMAMAV
                     AAALDALHFSEMVGCIAGDDTIMCAIRTVDDTILMMDKLKKLITG"
     gene            14963..16216
                     /locus_tag="JWH23_RS22530"
     CDS             14963..16216
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /locus_tag="JWH23_RS22530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_349772597.1"
                     /transl_table=11
                     /translation="MLSELHVKNLALIEKADVEFKEGLNVLTGETGAGKSIIIGSVTIA
                     LGGKVPKDMIRNGAEYAYIELIFTVSDERKIHLLKEMDVFPDQDGIVIISKKIMPSKSL
                     SKINDETVTAGKLRDITGLLIDIHGQHEHQSLLYKSVHLEILDRYQEKKSHELKMNIAK
                     TYREYVRLKERLASFQLDEDTRLREMDFIRYEIEEIENAGLREGEEEELSSKYRLFLHG
                     KKIMESLSVAYDAVNSDVTGRALKEVEAAAVYDERLLTIRDQLFDVDSILSDINHEITS
                     YLEDLSFDEEDYQKIEDRLDLIHNMEAKYGKGVDLIFKNLEEKKIRLSELEDYDRLKQE
                     TEKEIHLTEDKLEDLCGQLSCMRKETAKELTEKIKEGLHDLNFIDVNFDMEFGRLDHYT
                     ANGFDEAEFMISANPGRP"
     gene            16249..16632
                     /locus_tag="JWH23_RS32480"
     CDS             16249..16632
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS32480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_349772598.1"
                     /transl_table=11
                     /translation="MSRIMLAIKTVLADSDDIPTLIFDEIDTGISGRTAQKVSEKLSYI
                     AANHQVICITHLPQIAAMADAHFEIKKSAEAGKTTTGIQALQDEEIVEELARLLGGAEI
                     TDAVKVNAREMKRLAAEKKLKKS"
     gene            16758..18032
                     /gene="spoIVB"
                     /locus_tag="JWH23_RS22535"
     CDS             16758..18032
                     /EC_number="3.4.21.116"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIVB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553741.1"
                     /locus_tag="JWH23_RS22535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIVB peptidase"
                     /protein_id="WP_277406288.1"
                     /transl_table=11
                     /translation="MAKKKFHKLIVRAFWISLIFCIGFTWFYMKRVIPDKLNIVAAEEE
                     QFHFSMPFDVTLHSDSEEVVLNNGSNIPSDQIHLQVNQPFSVYSKNLGSYKLGLKLFGW
                     IQLKDIQVNVVDTKYAVPCGTPVGIYLKSNGVMVIGTGDVTKKDGMVVEPAFGVLQSGD
                     YLESINGVPLSNKETLMNELNKIGASEAVLKVRRGDDNIDIKMNPIETEDGSYKLGVWV
                     RDDTQGIGTMTYVDMNGQFGALGHGISDSDTGNVVQITDGSLYETAILGIEKGTFGKPG
                     VMSGIIYYGPGSNLGTIKENTEEGIFGTVNDRFKQGVSQDAVPIGYRQDVKKGKAYIRS
                     DVSGKVRDYEIEIQRVDYSTTHKSKGMVIKVTDPDLLALTGGIVQGMSGSPILQDGKLI
                     GAVTHVFIQDSTRGYGIFIENMINH"
     gene            complement(18102..18452)
                     /locus_tag="JWH23_RS22540"
     CDS             complement(18102..18452)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781658.1"
                     /locus_tag="JWH23_RS22540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_277406289.1"
                     /sec_met_domain="PF05402 (E-value: 2.1e-11, bitscore: 42.5,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQTDKKSENYLDRIPIRTEHIHWSVEESGLVTLDVENKGVFHRF
                     AQRFFHKPKVSHIHLDPMGSFVWCLIDGSRDILSLSALVKEQFGEEASPLYERLSKYFH
                     ILNSYGFIRFKK"
     gene            complement(18449..20360)
                     /locus_tag="JWH23_RS22545"
                     /pseudo=""
     CDS             complement(18449..20360)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875950.1"
                     /locus_tag="JWH23_RS22545"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="oligopeptide transporter, OPT family"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKEQNDFRPYIPAEKVTPEFTVTSIVLGIILAVVFGAANAYLGLR
                     VGMTISASIPAAVISMGVIRVMMKKNSILESNIVQTVGSAGESLAAGAIFTLPALFLWA
                     AEGKMETPGILEITLISLIGGILGVLFMVPLRNALIVKEHGILPYPEGTACAEVLLAGE
                     EGGANASTVFAGMGFAAVFKFIIDGIKLVPGEVSFRVKGYAGEIGTQIYPAVMSVGYIC
                     GPRISSYMFAGGIISWLVFIPAIVLFWSRFNPVSRYCSHWRAVCCQRSRRHLGNLHPLH
                     WCRSSCSRRYYQPCKISSINYPHIP"
     misc_feature    20580..20595
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.44"
                     /tool="antismash"
ORIGIN
        1 aagcagaccg cagatttaat cggttattcc gtggctacta tttcagaagg aggaaaactg
       61 gctgaccagt ctgcgggaat tttaaaaaca gtggaagaaa aagcaggtct ggttgatgac
      121 attattcatg agattgccct ggcttccgaa gcacaggttc aggcgatgga agagattaac
      181 cagggactgt ctcaggtatc cacagtggta caaaataatg ctgctacagc cgaggaaagc
      241 tctgcttcca gtgaggaact ggattccctg gctcaggcac tgaaacagga agtggagact
      301 tttaaactga gaaacagcat gtaaaaataa cagagtttat aggaagtccg ttttttacgt
      361 cagaattatg gcgcaaagaa cggacttttt ttaagtcata atttgtccca cttttcatat
      421 attgaggtaa gaggagggat gagagtggag agaaaagacg agctgatcaa tctgttttct
      481 aaaaacgtaa gagaaatctt aaacaaggta gccttaaact tcgacgaagt ccaggaaata
      541 aggctccgtg tggctgcccc tcttatgctg gtgtacagaa acgaagagta ttatttaacc
      601 ggatcaggaa atatcagcaa aaaactggaa gaagcctatc acattacaaa aaacgaattg
      661 aaggaaacca tggagtatat gagcaactac tccctgtatg cgtttgaaga agaattaaag
      721 cagggcttcc ttactgtaca aggggggcac agaatcggga ttgcaggcaa gacaatcctt
      781 gatgagtccg ggataaaaac catgaaatac atatctttta ttaatatccg cctttcccat
      841 caggtaaagg gctgtgcttc tcccatactg ccttttgtct ataaaaatca aagagaggtc
      901 tgccataccc tgattatttc tccgcccaga tgcggaaaga ccacacttct tcgggattta
      961 atccggcaga tatcagatgg ttccagagag tttgaaggta tgaacgtggg agtagtggat
     1021 gaacggtctg agatcggagc ctgttaccag ggagcgccgc aaaatgatct gggaatccgc
     1081 actgatattt tagactgctg tccaaaagca aaggggatga tgatgttaat ccgcaccatg
     1141 tctcccagag tgattgcagt ggatgaggtg ggaagcaggg aagacttaga tgccatcgaa
     1201 tacgtaatga actgcggatg caaactcatc gcaacggtac acggcagttc catcgaagat
     1261 ttaaaacaga agccggtgct aagaagtctt ttggaggaac ggattttcga acggtatatt
     1321 gtcttaaaca acttaggcag aatcggaaac attgatcaga tctttgattc cagaggaacc
     1381 cagttgtatc aggccaatat atttggaacc ggggtccggc cggacaggca ggagagcagg
     1441 gcttatgggt aatatatggt taagactggc aggggcagcc cttgtaatcg gatcctgttc
     1501 cggtcttggt ttttatatgg catcccagtg gaatgagcat ttaaaaacag tggaacattt
     1561 aagaaaaatg atcttccttt taaaagggga aattgtctat gccaattctc ctctggctga
     1621 agcctttgaa cggacaggca ggaaagcggg ggggcagatg ggagatttgt ttttaaaggt
     1681 gtcagatcga ttgatggggc aaagagggga aagcttttac ggcatctggc aggaagaaat
     1741 tgacggtctt ccaaaagagg tgtgtctatc cagtgaggat aagcagaatt taaaggggct
     1801 tggggaacat ctgggatatt tagataccgg aatgcaggag aggacaatcc ttctttatct
     1861 ggaacagctg gatctgtcaa ttgattatct aagaaatcat aaacaggaaa agagccggct
     1921 ttatacaagc cttggcatca tgggaggttt attcctgacc attgtaatgt attaaggagg
     1981 atagcatggg agtcaatttg atatttaaaa ttgcagcagt gggaattctg gtttccgtca
     2041 tctgccaggt attaaaacat agcggacggg aagaacaggc attcttaaca agcctggcag
     2101 ggctcattct tgtgctgttc tggctggtac cttacattta tcagctgttt gaatccatta
     2161 aaaatctgtt tgcattgtag gtgagaatat gacggttgtt tccatagctg ttgtggggat
     2221 tgtggcagtg cttctggcag tttccctaaa aggaatgaag ggggaatacg gaacctttct
     2281 ggtccttgca gcagggtttt ttatcttttt ttatggggta gggaagctta gcacaattct
     2341 tgataccatg aaagaaatcc agtcctacat aaaaatcaac agcgtctatc tgtccaccct
     2401 ggtaaagatg ataggaatta cctacattgc agagtttgca tcaggaatct gtaaagatgc
     2461 aggctacggt gcagtgggaa cgcagattga gatatttgga aagctttccg tactggcagt
     2521 cagtatgcca attcttctgg cactgattga aactcttcag gtatttttat catgacagga
     2581 ggaaggaaca tgaaaagaag gtttttattt cttctgtgtc ttctgtgttc tttttcccgg
     2641 cagtacttcc tggggagcgg aggctccggc aaaagagcag gaaacagagg tcacccaggg
     2701 gcttgatctg gatcagtatg atctgactga cattcagaaa tttctggatc agagaaatga
     2761 taatcagggg ctgaatcttt cctttcagga tttaatgaaa agtcttatgg atgggaagtt
     2821 aaatgatgta ctcagccaga ctggaaaagc gcttaaaaaa atgcttatgg gcgaaatcga
     2881 aagcagcggg catatgatgg gccagattct tgtacttggc attatcgggg ctgtcttttc
     2941 taatttttcc agtgtattta caggaagtca gatctccgaa acaggttttt ttgttactta
     3001 tttattatta ttcacctatc tggcagccag cttttttacc agcattacca ttgtaagcgg
     3061 cgtggttaac cagatactgg gatttatgag agtgctgatg cctgcgtatt ttctggcggc
     3121 ggcatttgca ggaagcagtg ccaccgcagt ggcatcttat gaatttaccc tggttatttt
     3181 atctgcttcc cagtggcttt taggaacggt actgttatca ctgatccgtg tatacgctct
     3241 tctggttatg gctggtcata ttgcaaaaga agacatgctg tcaaagctga ctgaactgtt
     3301 agagcaggtg gtttcctgga gcttaaagac tttagtcgga ctggtactgg gattccagat
     3361 gatccagtcc atggtgcttc cttatgtgga ttccttaaag acgggagcag ttcagaagct
     3421 ggtgggtgcg attccaggca ttggacaggg ggtcagctct gtggcccaga tggtattagg
     3481 ctctggtgta ctgattaaga ataccattgg agcagccggt atcctgattc tcattattct
     3541 aaccatcgtt cccattctaa agctagttat cttaatgctg ttttatcaat gtgtggcagc
     3601 ccttttgcag ccagtctgtg ataaacggat tgtatcctgc atttccgata tggcaaaggg
     3661 acataaaatg cttttgtccg tcgcgatatc ctgcatgatt ttattcatcg ttaccatagc
     3721 acttgtgtgt gcttctacca atgtaaccta ttattccggg tgagtggcat ggatcaatta
     3781 tatgaatgga tcagaaatat cacttattat ctgatcttta tcactgtggt agaaaatctt
     3841 ctgccgaata aaaagtatga aaaatacgtc aaattctttg ccggtatggt gctgattctt
     3901 cttgtgttaa agcccgttac aggcggatta agactggatg ataaactggc ctactcattt
     3961 gaagcaataa gccttaaaaa ggaagcaggt gagttaaccg gaaaagtttc aaaaatggag
     4021 gaaaccagac tgaaacgaat gatttccggt tatgaaaaag cagtgggaac tgatttggag
     4081 accatggcgc agacagcagg ttattcctgc aaagaaacca gtgcagaaat taacagtgat
     4141 cagtccagtg accggttcgg tcatgtggta aaggtttccc ttgtgctcac ctctgggaaa
     4201 gaggaaatgc aggcagagga agcagcttct gttggcagta aaatggatcc tgttaagaag
     4261 gttgaggaaa taaaaacatt aaaataactg gaaaagaaac ggatcaaagt gctgaaataa
     4321 agccggaaga tggaaaaaaa gaagagcaga aagaaaattc cagtctatct ggtcttagaa
     4381 ggaggattgc ggaatattat ggattggagg aacaggatat tgaaattcaa atggaagatg
     4441 ggtaaggata aattgttaat cctgctggcg gttggaatga tgatcctgat ccttacattt
     4501 ccttctggtc ccggaattgc aaagaaagtg gaaaagactg cgcagaaaga taataataag
     4561 caatccactt tacagcaggg aattgtaaca cagcctgcag acggagatgc tgccgcttcc
     4621 gtttccgctg accgcactta tgaagaagaa ctggaagcca gggtgaaaaa aatattaaaa
     4681 accgtggatg gagtcggtca ggttgatgtg ctcattgtag taaaatcttc aggggaaaag
     4741 gtactgagag tggataaaga taactcaaat tcctccaccg aagaaaatga cacatcagga
     4801 gggtcgagaa agattacaag cgcagaatta aaagagagta ccattcttac cggccaggga
     4861 gaaaatacgg ctcccattgt ggagaaagaa atacgtcctg aaatagaagg aattattatc
     4921 agtgcccagg gcggaggcag tcctacggta aaagctgaaa tttccagtgc catggaagca
     4981 ttatttaacc tgcccccaca taaaataaaa gtattaaaga gggtggaata aaaaggagtg
     5041 tcaggtatga aaagttggaa aacaagcaat gaacccaatg tcccgaaaac cccaaagaaa
     5101 atggatatga agaaactgtt tcgaagaaac cagattatca ttacaactct ggcagtcatg
     5161 attgctgccg ccggatattt aaattatgcc ggaaagcagg atgcagcctc cggaaccgat
     5221 gtgtatgaag ccggaatgac agacatttcc gaagaagaca ttttagcaga gaaccaggca
     5281 ttaaatggca gtgattccag caaggatatc gccagtctgg atcaggatgc cgcagatatt
     5341 gacaaactgg cagcagccga ttctgcgggc agcgcagata aaacagcagc cggaagtacc
     5401 caggtagcag ctgggaacga aacagctcaa agtcttgcag caggtgagac ccaggcagca
     5461 gccagccttg cagaaggtga aacacaggca gctgataccg gtcttgacaa tcctggtgag
     5521 gctgttttaa ccagcggtat gaacgtagca gattacatat caagcgtgca gttaaacaga
     5581 gaacaggtaa gggccagaaa taaagaaact ctgatgaatt taatcaacaa tccaaacatt
     5641 gaagaggctg ccaaacagca ggcaattcag cagatggttg atatgacagc gattgccgag
     5701 aaggagaatg cagcagaaac ccttcttctt gcaaaaggat ttgcggatcc tgttgtcagc
     5761 atctcaaacg gaaaagttga tgttgtcatc aatgccgcca gcatttcaga tccccagcgt
     5821 gcccagattg aagatatcgt aaagagaacc gaagtgggag cagagagcat tgttattact
     5881 cttatgaaac tggaagagta aaaagccttt gcaaacagtt gacaattttg ctataatgat
     5941 ttcagaaccc agaaatacta aacaggaggg aatcgaagtg gcagaattgg aaaacagaaa
     6001 tactcataaa gtatatgaaa aagataaaat cggcgaagtt cagatcgcag atgatgtagt
     6061 cgcgatcatc gcaggtcttg ctgcgacgga agtagatggt gtggattcca tggcaggcaa
     6121 catcaccaac gaactggtgg cgaagctggg catgaaaaac ttatccaaag gtgtcaaagt
     6181 ggaactgaca gaggaacatg tttccgtaga tttgtcctta aacataaaat atggttacag
     6241 tatcccaaac gtcagtgaaa aagtccagga aaaggtgcag aatgccattg agaacatgac
     6301 tggcctttcc gttcttgatg tgaacatccg gattgccggt gttgcactgg atgagaacaa
     6361 ataattttat aatttacagc gggaataacc ggtcatacgg ttcattcccg ctgttttttt
     6421 taccgaaaag atgaaattat ccttgtaatg atatagggca ggatttataa tggacgatat
     6481 tgtgaatcga aagattctta aggacaggtg ttttttaaat atgatgaatc gtagaaacgg
     6541 ggggcactat ccgccgggtt tgctgatcct ggcaagtctt gtgatatctg tgattatcgg
     6601 aatctgcgtt ttaaacttct attattttca tgaagtggaa cagagtctgt ttcagcagac
     6661 ccacagagat ctaaagcaga agaatgataa agcactgaat tatatagaaa ccatgataca
     6721 gaccaaattc gaatggctgg aattgtttgc attttactgt gatctgccca atggatcagg
     6781 caacgaacag tggtggcagc aggttcagga ttacgaaaaa gaagattcca ggtttggtgt
     6841 tgcagatacg tcaggtactc tttattatgg taatcgtgaa acccaggaca tttcttcccg
     6901 tgactattat aaaaaagcgt tagacggtga aaagaatata tccggattgc tgaaagaaag
     6961 ctttaagggc caggacagta tcattttaac cgtccccatt atcagagacg gcgttgttaa
     7021 gggtgcggcg tgtctggagt tgtcttccga taagctgaaa cagtatttaa gcgatacgga
     7081 tttaagccgc tacggtgcca atatgatatt tcaaaaaaga tggagagatt ctgatttcct
     7141 gtacggcatg tgaaaatcag atttctctgt ttgagatgct taaaacaagg aaatttggac
     7201 cgggtcagac attttcggag atggaagcag gaatcagcca ggggctgtcc ggttatatta
     7261 cttattatga aaatgattac cgccgccttc tttattacca gcccatgggg attaatgact
     7321 ggtatatggc gacctttgtg gagacggaag gatatgagaa tacgtttcat aaaatcaaat
     7381 ccctttctgc aaaatttata gcaggatcaa ccttcctttt attttgcgga gtgatcctga
     7441 tccttttaat tgttcacacc agaaaaaaag aagaaaagag actgcaaaag gattatttaa
     7501 caggagttta taccagagaa acagcaagaa aactggtgga gcatggatta aaagccggcg
     7561 gaaaaaaccg cttttatgca tgtatgttcc ttgacatcga tgattttaag aaaataaacg
     7621 atacctttgg tcaccataaa ggggatctgg ttctggttca ggcgggacag atattaagtg
     7681 cctgcaccag acaggaggat gtaattgtcc ggttcggagg agatgaattc tgtatctggc
     7741 tttatggaat gagaggaaga aaacagcccg aagcaattgc ccagcgtatt attaatgcgt
     7801 ttcatgtttc cggaaaagta catgctagta ttggaattac tttgattgga gagaatgaaa
     7861 ctgaatacga tgccatctta aaacgggcag atcaggcatt atacgaggca aagggaaaag
     7921 gcaaaaacca gtatgctgtt tccaattaac attccatact ggcacgcaaa agtttctatt
     7981 gtataaaaaa ccctgttaag gtataataat tccaaacgtc cagttaaatc accaatagga
     8041 ggaccatggt aaatgaccag aagtaaaatg cgtgaacact gctttaagat gcttttctgc
     8101 gctgattttc accccgcaga ggagaaactt tggcagatta atcaatattt tgaggaacca
     8161 aaagaggatg atctcaatac agaaggtgtg gaagagatta ttcatgatgt ggatatgagt
     8221 gaagagaatt ctgctttctt aaaagaaaag acagaagcaa ttatgaataa aattccggaa
     8281 ctggatgaaa aaatcaatga agtagcagaa ggctggaaaa caaaacgtat gggaagagca
     8341 gaattaacca ttcttcgcct tgctctttat gaaatcttat tcgatgatga ggtgccggaa
     8401 aaggtagcca tcaacgaagc agtagaactg ggtaaaaaat acggcggaaa cgaagctccg
     8461 gctttcatta acggagttct tgcaaagctg gtgtaagtat ggcaggtgtc tattcagtaa
     8521 cccagattaa tggttatatt aaaaatatgt ttgcccagga ttttgccctg aaccggattt
     8581 ccatcaaagg ggaagtatcc aactgcaaat atcatacttc cggtcatatc tattttacct
     8641 taaaagacaa agcctcttct atttcggcag tgatgtttgc cggatccgga aaaggtcttt
     8701 cctttcatct ggaagatgga cagcagatca ttgcaaaggg aagcgttgag gtttatgaaa
     8761 gagacggaag atatcagctt tacgcccagg aaattacgaa agagggaatg ggagatttat
     8821 tcgaacgttt tataaagctt cgtaatgaac tggaagagat ggggatgttt tcccctgaat
     8881 acaagaaaga aattccagga tatgcaaggc gtatgggtgt ggtaactgcc cccacaggcg
     8941 ctgccatccg ggatattatg aatatatccg ccaggcggaa cccctttgtg cagctctatc
     9001 tttaccccgc tcttgtgcag ggaagcggtg ctgcagaaag tattgtaaaa ggaattgaga
     9061 ccttagacca gatgggactt gatgttctca ttgtgggaag agggggcggc tccattgaag
     9121 acttatgggc ttttaatgaa gaagcggttg ccagagcaat ctttaactgc tccgtaccgg
     9181 taatttctgc agtcggtcat gaaaccgatg tgaccattgc ggattatgtt gcggacatgc
     9241 gtgcacccac cccttcggca gctgcggagc tggcagtatt tgattataaa caatttaaag
     9301 aacagacaga gagcatccgc tatacattaa aaaaccatga tggagcgaaa gctggagcgg
     9361 tatcgtttct ctgtgaacca atatggatta aagttaaagt actttgatcc gaagcgaagt
     9421 attcgtgaac agcgccagcg tcttcatgat ctggaagaga agctgaaaag ccagctttta
     9481 atacagatag aggcagagcg caggcaactt gacgaatccg ctgttaagat gagtcagctg
     9541 atggagaagc agactttaag agacaggcac aggctggaac ttcttgccgg acgccttcat
     9601 gcctgttccc cgctggcaaa aataggcggc ggctacggtt ttatcactga ttcagggaat
     9661 cacaggattg attctgtcat gcaggtaaag gccggagatg ctcttcgtct taaattgcgg
     9721 gatggaaata tccatactct ggtatcagaa gtggagccac taacagtgtc agaggcagaa
     9781 aaataaggag aaacaggctt atggcagtaa aaaaagaaag aaccattgaa gagaccttaa
     9841 aggacttgga ggagctgatc aaaaagctgg aaagcggaga gagctcttta gaggaatcct
     9901 ttgaatatta tgagacaggt atgaaacttg ttaagtcctg caatgataaa atagataaag
     9961 tggaaaaaga taattgtact ggaggaaaat ggtgaagaac atgaactttg aggatgagtt
    10021 actgaaacgg accaaagatg tgtgggagat tgtggaaagt tttcttccgg ctgtagaggg
    10081 acatcagagc actgttcttg aggcaatgga ttacagtata cgggcaggcg ggaagcggct
    10141 gcgtcctatg ctgatgaggg agacatactg cctttttgga ggtatgggaa aagagataga
    10201 accatttatg gcagccatag agatgattca tacctcttct ctgattcacg atgatcttcc
    10261 ctgtatggat aacgataccc ttcgcagagg acttcctact gcctgggtga aatttggata
    10321 tgatatggcg gtgctggcag gtgacggtct tttaatttat tccatggaga cggcagcaaa
    10381 ggcacttgat ttttcggaca ggccggaccg ggtcgcaaga gctatgggaa ttcttgcgca
    10441 aaagacaggt atattcggga tgataggcgg ccagacggta gatgtggaac tggctggtaa
    10501 gccgattcca ggcgaaaaac tggactttat ctaccggtta aagactggag cgcttttaga
    10561 ggcgtccatg atgatcggag cactccttgg cggagccagt gaagacgagt taaaggcagt
    10621 ggaagagatg gcatcaaaaa tcggacttgc ttttcagatt caggatgata ttcttgatgt
    10681 aacaagcaat gccgaagtat tgggaaaacc cgtattcagc gatgaaaaga acaataaaac
    10741 cacttatgta acattggagg gaatggaaca ggcgaagatg gctgtggaga gaatttccgc
    10801 agaagcagtt gcctgccttc aaaagctgcc gggagagaac cgctttctgg aagaactgat
    10861 ccggatgctg gttaaccggg aaaaataaag gattaaaaaa ttatgatgct ggaactgatt
    10921 aacggacctg acgatataaa gaaattctcc aaacaggagc tgaccatatt aagtcaggaa
    10981 atccgtgatt ttttaataga aaagataagt accacaggcg gtcatctggc ctccaattta
    11041 ggagtagtgg agctgaccat ggcaatttat cttgcttttg atctgccaat ggacaaaatt
    11101 gtctgggatg tagggcatca gtcttatacc cataagatct taagcggaag aagaatggaa
    11161 tttgatgaac tgcgccagta cggcggcatg agcggttttc caaagagaaa ggaaagcccc
    11221 tgtgacgcct ttgataccgg gcacagttct acttctattt ctgccggact gggacttgcc
    11281 caggcaagag atgtgctggg ggaaggtcat tttgtagttt cagtcatcgg tgacggttcc
    11341 ctgacgggag gcatggcata tgaagcactg aataatgcag cccagatgaa gaaaacttcg
    11401 ttattattct caatgacaat aatatgtcca tatccgaaaa tgtaggcggc atgtcctcct
    11461 atctaaacag tatacggact ggaaatggat atctggattt aaagaaacat gttaccaata
    11521 cgctttcaag gattccggtt atcggagaac ctctgattga gaagatcagc cggactaaaa
    11581 atggcattaa gcagcttttg attccgggaa tgctgtttga aaatatgggt attacctatt
    11641 taggaccggt tgacggccat aatttaaagg ctctgacaag aaccctgaaa gaggcgaaga
    11701 aactggatca caccgtactg gttcatgtga ttaccaaaaa aggaaaagga tatgctccgg
    11761 cagaaaaaaa tccgtccaag tttcacgggg tggatccctt tgatatcctg actggcaagc
    11821 ctaaaaaaga aaagaagaat ttaagctata cagatgtctt ttctaaaaca atctgccgtc
    11881 tggcaaaaga tgataaaaag attgtggcgg taaccgctgc gatgccggat ggaaccggat
    11941 tgaaacggtt ctcaaacctc tatccggacc gcttttttga tgtggggatt gcagaagagc
    12001 atgcagtgac ttcggcggca ggaatggcag caggcggttt gaaaccggta gtggctgttt
    12061 attcctcttt tttgcagaga gggtttgatc aggtacttca tgatgtgtgt attcagaatc
    12121 ttcctgttgt attcgcagtt gaccgggcag gactggtagg cagcgatggt gagacccatc
    12181 agggaatttt tgatctgtcc tttttaagtg ccatacccaa tatgagcatt tttgcaccga
    12241 aaaatgactg ggagctggca aacggactgg agttcgcatt ttcccttgac agtcccattg
    12301 cggtacgtta tcccagaggg ggaagcctat caggggctgg aagattttaa atcacccatt
    12361 gaatacggga aaggcgaaat catttatgaa gaaacggaca ttgcccttct ggcagtagga
    12421 agcatggtaa gtacaggaga gcatgtaagg cagaaactga aagcggaagg ctggaactgt
    12481 actcttgcta acgggcgctt tatcaagcct tttgataaaa aactgattga tcacctggca
    12541 aaaaatcact ggctgattgt gaccatggaa gagaatgttc ttcagggcgg atatggaagc
    12601 ctgatcaatg gttatataca tgaacactat ccaaaagtaa aggtactaaa cattgccctg
    12661 ccggatgcat acgtggagca cggcaacgtt tctctgcttc gaaaaggact tggaattgac
    12721 agcgattcca taatctggcg catgaaaaag gaataccttg atatggagcg gcagaacagg
    12781 gaatggaagg ataagatgaa gtaaaatgaa agaacggttg gatgtgctat tagtaaaaca
    12841 gggacatgca gagtccagag aaaaggcgaa agccatcatc atgtccggga tcgtatttgt
    12901 gaatggtgac aaagaggaca aagcaggttc cacatttgaa gaaactgcac ttattgaggt
    12961 aagaggcagc actttaaaat atgtaagccg gggcggatta aagcttgaaa aagccatgac
    13021 ccattttaat gttacgctgg aagggaaggt ctgcatggat gtaggctctt ctaccggagg
    13081 atttacggac tgcatgcttc aaaacggcgg ggttaaagtc tatgcggttg acgtggggca
    13141 tgggcagctt gcgtggaagc ttcgcaacga tgaacgggtg gtctgtatgg aaaagaccaa
    13201 catccgttat gtaacgccag aggatattaa ggacaggatt gaattttcat ccattgatgt
    13261 atcatttata tcccttacaa aagttctttt gccggttaaa aaccttttgt cagaagacgg
    13321 agagatcgtt tgtctgatta agcctcagtt tgaagcaggc cgtgaaaagg tgggcaaaaa
    13381 gggtgtagta agagacaaat ctgttcatct ggaagtaatc cagtcagtga tctcttatgg
    13441 aatcagcatt ggatttgaaa ttctgcacct ggagttttct cctattaagg gtccggaagg
    13501 gaatatagaa tatctgcttc atttaaaaaa tcatcccgct ggggagagct ttcctgagta
    13561 cagtgttaat ccggaaaaag tggtggaaga agctcacaaa gacttaagcg aaggcaaata
    13621 aggagtctga aatgaaacat ttttacgtaa tcatgaatcc ggataaaaaa ggagcccgtg
    13681 aaaccgcgga gcagatccgg gaatttctca ctgccagggg agcagtctgt ctgatcggca
    13741 gaggagaaaa ggtagaatgg caggcaggaa agcattacac tgactcagcc atggtgccga
    13801 aagaaacgga atgcgtgatt actctggggg gagacggaac gctaattcag gcagccagag
    13861 atttatccgg acgcaattta cccattcttg gaatcaacag agggactctg gggtatttaa
    13921 cccaggtttc cagaacagaa gacattgaat cggcgctgtc agccctgttt ttcgatgatt
    13981 ataagctgga agagcgtatg atgctaagcg gaagtgcatt cagagacgga aaagagattt
    14041 accgtgatat cgctctaaac gagatcgtca taacacgagg cgaatacttg aagatgcttc
    14101 agtttaaagt atatgtaaat ggggaattct taacggagta cagagcggac gggctgatag
    14161 cagctacgcc cactggatct actgcctata atttatcggc aggcgggccg attatagtcc
    14221 ctgattccaa gatgctggtg cttacaccca tctgttccca tgccctcaat gccagaagta
    14281 ttgtgctttc tgcagaagat aaaatcagga ttgaaattat aggacaggaa ggaatcagcc
    14341 aggcagcggt atttgacgga gattcggcag tcagtctttt acctggggac tggattgata
    14401 tagagcgtgc ccagatcaag actatattag taaaattaaa aaatttatca ttttagataa
    14461 tctaagaaac aaaatggcgg gtatataagg aggtgacacg atgaaactgg aaagacatag
    14521 caaaatagta gaactgattg ggaagtatga aattgaaacc caggaagaac tggcggatta
    14581 tttaaatcag gctggttttg cagtaacaca ggcaactgta tccagagata tcagagagtt
    14641 aaagctgaca aaggtgcagt cagaatcagg aaaacagcgt tatatggtac ttcagaatca
    14701 gggatcattc agcgataaat acatccggat tttaagagat ggttatttat ccatggatat
    14761 ggcacagaat atactggtaa ttaagactgt atccggaatg gccatggcag tggcagcagc
    14821 gttagatgcc cttcatttca gcgaaatggt aggctgtatc gcaggagacg ataccatcat
    14881 gtgtgccatc agaactgtag atgacaccat acttatgatg gacaaactga aaaaactcat
    14941 tacgggataa aggaggcaag ccatgctttc agaattacac gtaaagaact tagcattaat
    15001 tgaaaaagct gatgtggagt ttaaagaggg actgaatgtc ttaaccggtg aaacaggtgc
    15061 cggaaaatcc ataattatcg gctcggtaac cattgcctta ggaggcaagg tgccaaagga
    15121 tatgatcaga aacggggcag aatacgccta catagagctg atctttaccg tcagcgatga
    15181 aagaaagatc catctgttaa aagaaatgga tgtatttccc gaccaggacg gcattgtaat
    15241 tatttctaaa aaaatcatgc cgtctaaaag tttaagcaaa attaatgatg aaacggtaac
    15301 tgcagggaaa ctgcgggata tcacaggtct tctcatcgat attcatggac agcatgaaca
    15361 tcagtcttta ctctacaagt cagtacatct ggagatcctg gaccgttacc aggagaaaaa
    15421 gtcccacgag ctaaaaatga atattgctaa gacctacaga gaatacgtcc ggttaaagga
    15481 acggcttgcg tcatttcagc ttgatgaaga taccagactg cgggagatgg attttattcg
    15541 ctatgagata gaagagattg agaatgccgg gttaagagag ggagaagagg aagaactatc
    15601 ctcaaagtac cgtctgtttc ttcatggaaa aaaaattatg gaaagtcttt ctgtggctta
    15661 tgatgcagtg aattccgatg tcacaggacg ggctttaaaa gaggtggaag cagcggctgt
    15721 ctatgatgaa cgcctgctta ccatcagaga ccagcttttt gatgtggact ccattttaag
    15781 cgatatcaat catgaaatca cctcttatct ggaggactta tcttttgatg aagaagacta
    15841 tcagaaaata gaagaccgtc ttgacttaat tcataatatg gaagctaagt atggaaaagg
    15901 tgttgacttg attttcaaaa atctggaaga aaagaaaatc aggctgagtg aactggaaga
    15961 ttatgaccgt ttaaagcagg agacagaaaa agagattcat ttaactgagg ataaattaga
    16021 agatttatgc ggacaattat cctgtatgag gaaagaaact gcaaaagaac tgacggaaaa
    16081 aatcaaagaa ggcctacatg atttaaattt tattgatgtt aattttgata tggaatttgg
    16141 cagactggat cactatacgg caaacggttt tgatgaagcg gaatttatga tttccgccaa
    16201 tcccgggagg ccttaaagcc attaaaatcc gtggcttccg gcggtgagat gtcaagaatc
    16261 atgctggcaa tcaaaacagt tctggctgat tctgatgata ttccaaccct tatttttgat
    16321 gaaattgata ccggtatcag cggaagaacc gcacagaagg tatcagaaaa gctttcttat
    16381 atagcagcca atcaccaggt aatctgtatc acccatctgc cccagattgc agccatggca
    16441 gatgctcatt ttgaaattaa aaaatcggca gaggctggaa aaaccaccac aggaatccag
    16501 gcactgcagg atgaggaaat tgtggaagaa cttgcaaggc tgcttggcgg agcagagatt
    16561 acggatgcag taaaagtaaa tgccagagaa atgaagcgtc ttgcggccga aaaaaagtta
    16621 aaaaagtcat aactggtcca aacaccaaaa ttaaatgaat tcaactaaaa tatgacagag
    16681 tgtttttaac gaattctcac atactatgag aggagtcaaa aaattgtctc ctttattctt
    16741 ctaaggaggt atgtgagatg gcaaaaaaga aatttcataa actaattgta agggcgttct
    16801 ggatcagcct gatcttttgt atcggattta cctggttcta tatgaaacgg gttattccgg
    16861 acaaattgaa catcgttgca gcagaagaag aacagtttca tttctccatg ccttttgatg
    16921 tgacgctgca ttccgacagc gaggaagtgg tattgaacaa tggttccaat attccttctg
    16981 atcaaattca cttacaggtg aatcagccgt tttccgtgta ttccaagaat ctgggaagct
    17041 ataagctggg attaaagctg ttcggctgga tacagttaaa ggatattcag gtaaatgtag
    17101 ttgataccaa atacgctgtt ccttgcggta ctccagttgg aatttattta aaatcaaatg
    17161 gagtcatggt aatcggtaca ggagacgtaa cgaaaaaaga tggaatggtt gtagagcctg
    17221 catttggagt tttacaatca ggggattatc tggaatcaat caatggcgtc ccacttagta
    17281 acaaggaaac actgatgaat gagctgaata aaataggcgc atcagaagct gttttaaagg
    17341 tcagaagagg cgatgataac attgatatta agatgaatcc catagaaaca gaggatggat
    17401 cctataaact gggagtatgg gttcgtgatg acactcaggg aataggaacc atgacctatg
    17461 tggatatgaa cggacaattt ggtgcactgg gccacggaat cagtgacagt gataccggga
    17521 atgtagtcca gatcacagat ggttctctct atgaaacagc aatactggga attgaaaaag
    17581 gaacctttgg gaaaccaggt gtcatgagcg gaatcatcta ttatggtcct ggatccaact
    17641 tgggaacaat taaagaaaat acagaagaag gtattttcgg tacagtgaat gaccgcttta
    17701 aacagggagt ctctcaggat gccgtaccca tcggttatcg gcaggatgtg aagaagggaa
    17761 aagcctatat acgaagtgat gtctctggaa aagtgcgtga ttacgaaata gaaatccagc
    17821 gggttgatta ctccaccact cataaaagca agggaatggt aattaaagtt acggaccccg
    17881 atcttctggc attgaccggg ggaattgttc agggtatgtc cggtagtccg atcttacagg
    17941 acggaaagct gataggagct gttacccatg tttttatcca ggactcaaca agaggctacg
    18001 gtatctttat cgaaaatatg atcaatcatt aacaatattt gccaggaaat aaaaggacag
    18061 aatcgtaaga gtcttacggt gccgtccttt tctttttctg tttatttttt aaatctaata
    18121 aatccataac tgttcaggat atggaagtac ttggatagac gttcgtatag tggagatgct
    18181 tcctctccaa actgttcctt tactaaggca gaaaggctaa gaatatcccg gctgccatca
    18241 ataagacacc atacaaagct tcccattgga tccagatgaa tgtggcttac ttttggttta
    18301 tgaaaaaatc tctgggcgaa ccggtgaaag acgcctttgt tttccacgtc cagtgtcacc
    18361 agaccgcttt cttcaacaga ccagtgaata tgttctgtgc gaatgggaat ccggtctaaa
    18421 tagttttcgc tttttttatc tgtctgattc atttcttcac tttcttccag attgtaaatt
    18481 taagcaggct taagatgaca agcccaaata aaatcagact tccaaaatcc agaacaaaag
    18541 aaggaagatt tagttttgag gacagatcca gagccttgtc caaaccaaat accgctaaaa
    18601 gcgcaagcag gataccaacc aggccttcac ctgcaatcat tccggaacag taaagcattc
    18661 cgtcgctgat taactgatct ttttcttttt tgtcttcttt tttcattctg tcaaagtata
    18721 aacgtaccag tccgcccacc ataatgcatg catttaactg gacaggaaga tacacgccaa
    18781 tggcgaacgg aagaacggga atacccagga tttccacagc tgcggctaag aagactccca
    18841 taaataccag cgcccaggga agattgttat ccataacacc ttcaatgatc atcttcatca
    18901 gcatggcctg aggtgcaccc agctgttcag aaccaaagcc ccatgctgca tttaacagat
    18961 ataaaacccc gccaatggca aaggcagcag caaccacacc gatcagctca ccaatctgct
    19021 gctttttcgg agtagctcca agcaggtaac ctgtttttaa gtcctgagag gtatcaccag
    19081 caatggcagc aacgatgcag atgatggaac cgatggcaat tgcgcctttc attccatgga
    19141 ttccgttatc tccagtggct tttaatgtga tggtggcgat aaggagcgtt gcaatggcca
    19201 tacccgatac cgggttattg ctgcttccta ccagacctac cattctggaa gatacagtgg
    19261 caaagaaaaa tccaaagatt acaacaagga tggctcctac cagacttacc ggaatgaccg
    19321 gaaccagcca gacaataatg gtaagaatgg cgatacctcc aagaattgtc tgaaagctta
    19381 aatcctgttc ggtacgaagt ccgctgcctg cattgttctg accaagtccc tgaatggcat
    19441 cacggaatgt gcggataatt aatggaagag attttacaag gctgataata ccgcctgctg
    19501 caagagctcc tgcaccaatg taacggatgt aagttcccca gatgccgccg gctcctttgg
    19561 cagcaaacag ctctccaatg ggaacagtac cgggatacag ggttaaatcg gctccaaaaa
    19621 agaacaatcg cagggataaa taccagccag ctgataattc ctcccgcaaa catataggaa
    19681 gatatccttg gtccgcagat atatcccacg ctcatgactg ccggataaat ctgtgttcca
    19741 atctcacctg cataaccctt cacacggaag gatacttccc ctggaaccag tttgatccca
    19801 tcaatgataa acttgaatac agcggcaaag cccattccgg caaatacagt ggaagcattg
    19861 gcaccaccct cttcaccagc taaaagaacc tctgcacacg cggttccttc cggataagga
    19921 agaattccgt gctcctttac aatcaatgca tttcgaagcg gaaccatgaa aaggacaccc
    19981 agaataccgc cgatgagaga aatcagtgtg atttccagaa taccaggtgt ttccattttc
    20041 ccttctgctg cccataaaaa aagagcaggc agtgtaaaga tagctcctgc agccagagat
    20101 tctcctgcag aaccaactgt ttgaacgatg ttgctttcca gtatggagtt tttcttcatc
    20161 atgactctga tcacacccat ggagatgact gcggcaggaa tcgatgcgga gatggtcata
    20221 ccaacacgga gacccagata ggcattagct gcaccgaaga caacagccag aatgataccc
    20281 agaacaatag aggtaacagt aaattccggt gtgacctttt cagccggtat gtacggtctg
    20341 aaatcgtttt gttctttcat ttgtatttcc ccctaaaatt tttatacagt accaattata
    20401 tccatttatt taagatgggg caagtgttat tgtgcttttt tgataattaa aaataataaa
    20461 ataatatttt ttatcagcat aatgcttaaa ttatcattga taaaaaatag atctaatctc
    20521 tatgaaaagc tttaaatccc ttttcttatg tgttagtata agagatagta gcattgtttt
    20581 tgatgttttt ttgacgacaa gtagtttcaa ttttcaacac cggtaaaaaa aagggggaaa
    20641 cgtggattca gaccgggcaa gaccttttct tcattcgaca gtgggacagt tttgacagaa
    20701 tcccatattc gataaaataa acgtttttcc tgtcgaataa tgcgatagaa tcacgttgcg
    20761 gcataagcta gggcaagatt ataatggcta tacactagtt ttcatttatt ttgaagaatc
    20821 aggaggagag tatcaatatg tcagaaatca gtattgctat tgcagatgat aacctgcaga
    20881 ccttaaat
//
