LOCUS       NZ_CP070896            20291 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Lacrimispora xylanisolvens strain ASCUSBR21 chromosome, complete
            genome.
ACCESSION   NZ_CP070896
VERSION     NZ_CP070896.1
KEYWORDS    RefSeq.
SOURCE      Lacrimispora xylanisolvens
  ORGANISM  Lacrimispora xylanisolvens
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Lacrimispora.
REFERENCE   1  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Hungatella xylanolytica ASCUSBR21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) R&D, Ascus Biosciences, 6450 Lusk Blvd E209,
            San Diego, CA 92121, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAY-2019
            Assembly Method                   :: Canu v. 2.1.1
            Assembly Name :: Hungatella xylanolytica ASCUSBR21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017084465.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,443
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 686
            CDSs (without protein)            :: 686
            Pseudo Genes (ambiguous residues) :: 0 of 686
            Pseudo Genes (frameshifted)       :: 599 of 686
            Pseudo Genes (incomplete)         :: 95 of 686
            Pseudo Genes (internal stop)      :: 24 of 686
            Pseudo Genes (multiple problems)  :: 30 of 686
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:11:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3911481
            Orig. end                         :: 3931772
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20291
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RRE-containing"
                     /region_number="8"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..20291
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20291
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10291](+)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10291
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     misc_feature    complement(255..269)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.53"
                     /tool="antismash"
     gene            1010..2383
                     /locus_tag="JWH23_RS21690"
     CDS             1010..2383
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212868.1"
                     /locus_tag="JWH23_RS21690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nitrogenase component 1"
                     /protein_id="WP_277406173.1"
                     /transl_table=11
                     /translation="MLELTPKEIMENRHMTINPCKTCEPVGAMFCALGAEKCMPHSHGS
                     QGCCSYHRTVLSRHFKEPAIASSSSFTEGASVFGGRSNITTAVKNVFDIYKPDIMAIHT
                     TCLSETIGDDVGSYIMDMEVPEGKTVLYASTPSYEGSHIQGFSNMMIGFMRYMTKKTGT
                     PNGKVAIFPGWVNPGDDRELKKIAGLMGAEYIYFPDHEGILDHPMDGKSEYFHENAGTK
                     AKDIAAMGDCEKLISMGIYCSLEPSEYLKKEYKVPFSNIPVPVGVKLTDQYILELQKFT
                     KKEVPKELEDERGRLVDLMLDSHQYVYKKKAAIYGDPDIVYGFTALCLELGMSPKYIIT
                     GTPKAHWEKEIKELMDQYGVDESDYVVKADTDLFYLHQLIKNESVDLLLGSSYGKQIAK
                     AEDIPLVRMGFPVLDRYGNPIQATVGYAGAIRYTEKIVDAMMDRQDRDAADEDFDVVM"
     gene            2468..3823
                     /gene="nifE"
                     /locus_tag="JWH23_RS21695"
                     /pseudo=""
     CDS             2468..3823
                     /GO_function="GO:0005488 - binding [Evidence IEA]"
                     /GO_process="GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /gene="nifE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355186.1"
                     /locus_tag="JWH23_RS21695"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="nitrogenase iron-molybdenum cofactor biosynthesis
                     protein NifE"
                     /pseudo=""
                     /transl_table=11
                     /translation="METENSNRNILDARKSSIVVKDGCSGMGGGISCDSNSVSGSVSQR
                     ACVYCGARVVLNPITDAWHIVHGPIGCSSYTWDIRGSLTSGEDIYRNSFSTDLQETDII
                     FGGIRKLEAAVDELMEKEDNVKLIFIYATCIVGVIGDDVEALCKMKSEQYHIPVIPVKS
                     PGFSGNKSTGYRMACDAIMNVLLPHKSEEKRTAINILGDFNLAGEMWIIKNYFKEIGID
                     VISGFTGDTCYEDMIEAPKAALNIVQCAGSSTYLANRMEEEFGIPFIKVSFWNRRHHQF
                     LNQDGGSTSK"
     gene            3842..6475
                     /locus_tag="JWH23_RS21700"
                     /pseudo=""
     CDS             3842..6475
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170523.1"
                     /locus_tag="JWH23_RS21700"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="nitrogenase component 1"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSEVKPLVNLNINPCKMCMPMGSATAFYGLKNCMTILHGSQGCA
                     TYIRRHMATHYNEPVDIASSSLTEQGTVYGGSENLKKD"
     gene            6489..6797
                     /locus_tag="JWH23_RS21705"
     CDS             6489..6797
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429473.1"
                     /locus_tag="JWH23_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2Fe-2S ferredoxin"
                     /protein_id="WP_277406174.1"
                     /transl_table=11
                     /translation="MVQPKYHVFICTSCRINGTQKGMCYQKGAVDLIQSFMQEIEDRDL
                     SGEVVINNTGCFGICDKGPVVVVYPEGVWYGNVSEDDVERIFDEHFEGGNPVGDLMI"
     gene            6846..7829
                     /locus_tag="JWH23_RS21710"
     CDS             6846..7829
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277406175.1"
                     /transl_table=11
                     /translation="MIKIIDATLTMLEEYPLKKELVYEFARTLESLCIWGAVLTPRVYQ
                     LMQGDLPQGITWYMELPSPSESVKYPGIHFFFSSFCDGTGKFIRNIQVNDVSELRGLEC
                     ARPDNRLRLTGLDDLLIYESRDIYERGMKLLIQASPILYPENSCYCASAIAADYLQNHH
                     NGTVMTTFTGIGNKAATEQVILAMRVVERYKPNNSFEKLANLRDIFEAMTNTQILGHTP
                     VIGRKIFYIESGLHVDGVLKKPSNYESFPPELVGASRKVILGKHSGGTSIRYKLKQYQM
                     EDCYPVEHLLEMVKRLSIKKGGEVTDEEFLTMMSECDKYEKANQNS"
     misc_feature    6884..6898
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.68"
                     /tool="antismash"
     gene            7804..8631
                     /locus_tag="JWH23_RS21715"
     CDS             7804..8631
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /locus_tag="JWH23_RS21715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="homocitrate synthase"
                     /protein_id="WP_277406176.1"
                     /sec_met_domain="HMGL-like (E-value: 1.3e-63, bitscore:
                     213.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRRIKIVDTTLRDGEQCPGVVFSPSDKVRLALFLDSIGVSEIEA
                     GIYDVGTEGVHYISEIMKKKKQAAVSLWSKLNPENVEESARQKPDLIHIGTPVSYAQIY
                     NKLGKNKKWIENSLQECIEVARNYNIPITVGLEDASRADVAFLVSLIRLMRQKGVETIR
                     LADTVGVLFPRRAGELVREIKQTGIMIEVHEHNDFGMAVANSMVMALSGADMVDGTLLG
                     IGERAGNCNLYEFVHSAARKFDLNVSSADIRKGENLLKSILNGGKKNDSEDQI"
     gene            8644..9999
                     /locus_tag="JWH23_RS21720"
     CDS             8644..9999
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS21720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277406177.1"
                     /transl_table=11
                     /translation="MAEGILNLCRVLSCEGQVMVNGVYSFAASEVAGKLEGTDLFFNQG
                     IRLAAAAIGEINQAAGCGTGESARLAAQLLAYCERKSASGISPVLLARELKAAASALEE
                     FVGKNAIPLPDINTDLIEGITKDQKTAQMIVEGSEAGELVVKESMSGETSFEIIKGMRL
                     DGGLTVGAEGTVWDSLVLIVKTPITSFSSIYPLLQKTGQQNLFILADGIEGEALTLLSA
                     NVKQNRIHVRAMKAPGIGRRKEDLFEDAAAYTDTMVFDGIYPCQMEEISPAMLGRVKAV
                     TAAGSYTIIKGNADNNRICRRIDGIKERIGDPKTNYYDKQMLRGRIAGLMGCAPVIYAG
                     GYTTVQAKEEKRRIESALAYYQTVKQFGVLKKDDLINFQAGSEAEKFLTESMKKCFQNE
                     EISAYLMILMIQKICGLIVMWLTTGAVMVSTGYDREDLELIKSGVDIERLRS"
     gene            10001..10291
                     /locus_tag="JWH23_RS21725"
     CDS             10001..10291
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /locus_tag="JWH23_RS21725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_104434811.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 3.2e-08,
                     bitscore: 32.0, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7.2e-08, bitscore: 31.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIRKVKLKPGFALMELGGEFCIFDERDSQNGSLDGLPSVNEICI
                     FLWNSLERGAGVEELITLLSEEKNIDEEDARLELEEFLAKLIHGNVVEING"
     aSDomain        10016..10279
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_JWH23_RS21725_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.40E-10"
                     /identifier="RREFam002.1"
                     /label="JWH23_RS21725"
                     /locus_tag="JWH23_RS21725"
                     /protein_end="93"
                     /protein_start="5"
                     /score="32.0"
                     /tool="antismash"
                     /translation="VKLKPGFALMELGGEFCIFDERDSQNGSLDGLPSVNEICIFLWNS
                     LERGAGVEELITLLSEEKNIDEEDARLELEEFLAKLIHGNVVE"
     gene            10307..10840
                     /locus_tag="JWH23_RS21730"
     CDS             10307..10840
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021122.4"
                     /locus_tag="JWH23_RS21730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Fe-only nitrogenase accessory AnfO family
                     protein"
                     /protein_id="WP_277406178.1"
                     /transl_table=11
                     /translation="MEKIAVISKDQELVPFLYCNLVEIFEKKGEQWQAVHTASFSPITG
                     NTIEKLRKETEAIMSLAKDAKAVLCRELSGIPFSVFNQKGYCIFCSEKADQDTLDGMIK
                     DIEESDEKRRRKEEMIHNAGPVETQIPGIYFLDLVQLQTECPEVSSKKGTFALPFQYSI
                     SRITACLCPHSPMA"
     misc_feature    complement(10855..10870)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.27"
                     /tool="antismash"
     gene            10934..11491
                     /locus_tag="JWH23_RS21735"
     CDS             10934..11491
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS21735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277406179.1"
                     /transl_table=11
                     /translation="MQNYMVLDSGTLQTPYGILGGVDSFLCHPTGELSGVTLSEKNMIV
                     TQAGELVPAYTENGRRKKKPSVEFDKQGMVVSVALEEQQEVLTPIGELPVELIKFYPTG
                     ELHRIFILDGQISGFWTEEDERELNISLTFDLGFTEFQAMINGLCFYKSGDIKSITLFP
                     GGKDCHTDSHWNGGNQGGTFLI"
     gene            11472..11921
                     /locus_tag="JWH23_RS21740"
     CDS             11472..11921
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS21740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277406180.1"
                     /transl_table=11
                     /translation="MGLSLYESGNLKSVEPAEPVLVSTPIGKIAAYDPDQIGINADSNS
                     LSFTESGEIKGLITCDYSIYVQTEDGVMRKYSPHIKPHPLYDEVLTVSGIKVEFFPESG
                     EVLIENDRYDLESCGFTIEPFARPGMHCSPSDCANCSLCNKAMRE"
     gene            complement(11973..13424)
                     /locus_tag="JWH23_RS21745"
     CDS             complement(11973..13424)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1185: carbon
                     starvation protein A"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771604.1"
                     /locus_tag="JWH23_RS21745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carbon starvation CstA family protein"
                     /protein_id="WP_277406181.1"
                     /transl_table=11
                     /translation="MISFFLCLLLLIAGFFFYSKITEKVFGPDDRITPALSMTDGNDYV
                     PMGTPRIFLIQLLNIAGLGPIFGALAGACWGPSVFLWITFGTLLGGGVHDYMIGMMSMR
                     HKGASVSELTGSYLGTAMKQIMRVFSVILLVLVGVTFSTGPANLLAMLTPHVLDSRFWL
                     VVVLVYYFIATFVPIDKVIGKIYPFFGICLIVMALGVGGAILFKGYHIPEITLANLHPA
                     GTPIWPTMFISVACGAISGFHATQSPLMARCLTNEADGRKVFYGAMVAEGVIALIWAAA
                     GVAFYDGTGGLLTALGNGQSSVVYEICFQLLGPVGAVIAMIGVIACPISSADTAYRSAR
                     LTLADWFKLDQKPAKNRLLLTIPLLAVGSVLTQFDVQIVWRYFSWSNQTLAMIALWTAS
                     VYLFRKKRFYWITVLPATFMSAVSCTYILMAKEGLQISTTIAYPIGIIFAAACFGTFFY
                     TTVLGKGRHATASSSETEYAAAKSD"
     gene            complement(13533..13913)
                     /locus_tag="JWH23_RS32365"
     CDS             complement(13533..13913)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="JWH23_RS32365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_349772567.1"
                     /transl_table=11
                     /translation="MQTGFGSFFENDLCLPAHKFPINVNRTIILLDIRQIVYIETCCRS
                     CIIHTVSRDYTENQLLGEYEKRLSSCGFYRIHKSYLINLNYISEMFPWANNSMAVKMQG
                     FEKEILPVGREKIKQFKQIIGV"
     gene            complement(13796..14254)
                     /locus_tag="JWH23_RS32370"
     CDS             complement(13796..14254)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="JWH23_RS32370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator"
                     /protein_id="WP_349772568.1"
                     /transl_table=11
                     /translation="MKIAVIDDERPARKELIHQILSFLPESEIMEGDSGNAALELFGHH
                     YFDILFLDISLNDMDGTTLAAAARRMMPHAQIIFATAFSQYAVQAFELGVNNYILKPFD
                     PERLRCILEKCRQDLDHSLKMIYASQHTSFPLMSTEPLYCLISDRSYT"
     gene            complement(14235..14660)
                     /locus_tag="JWH23_RS21755"
     CDS             complement(14235..14660)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="JWH23_RS21755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_277406182.1"
                     /transl_table=11
                     /translation="MEKARFEEKLHIEIITDEKTNCLIPAFILQPLVENAIRYGFDKSA
                     NRYVSICACMEGAFIRISVSDKGPGIPETIIKHLYEGEPYGGVGLENVHKRLKSIYGQE
                     HGLHIERSDSGSDIFFTIPVSPESKYEGGLNYENCCD"
     gene            complement(14641..15957)
                     /locus_tag="JWH23_RS21760"
     CDS             complement(14641..15957)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /locus_tag="JWH23_RS21760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="WP_277406183.1"
                     /transl_table=11
                     /translation="MSSAMFFSLLLNIGLLVLIATLLTKIPIVRSMLLYDMHSAGCGIM
                     LAILFGLISIISTYTGVRTQGAIVNTRVIGVLAAGLLGGPYVGIGAALIGGIHRYLFDI
                     GGFTAAACAVSTLVEGLAGSLFSSRFKSGKMGSSSVFILTVFVEFLQMMIILLISRPFS
                     AAVQLVKLISLPMMTMNALGMLVFLGTFNMVFLEEDSQFAERMRLALGIVEQSLPHLRK
                     GLYSIKDMQAAAEIIFQSTSCAAILITDTEKIISKKANPELKDMNEDTVLLPILASIHD
                     RKPTIFHYARKGEPLYSILKNHIIVAAPIIEMNQVMGSLTMIVKKHWQQSQSDLNFASE
                     LARLFSTQFELSELEYQKKLRRKAELKALQSQVNPHFLYNSLNTISYVCRDNPDRAREL
                     LLTLSSYYRQTLEHNSYMHTLKTELMHITRYLGIGKSKI"
     gene            16130..16465
                     /locus_tag="JWH23_RS21765"
     CDS             16130..16465
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445300.1"
                     /locus_tag="JWH23_RS21765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phage holin family protein"
                     /protein_id="WP_277406184.1"
                     /transl_table=11
                     /translation="MMKLFIKYISIVLAIYLMSFIFNSVYIGSLGALLIMGMVLLLVNL
                     IIKPFLMLITLPANLLTLGLFSFIVNAWTIMIADSLVENISMGGFLNSLIAAFIITILN
                     NLFQDKN"
     gene            complement(16530..17492)
                     /gene="asrC"
                     /locus_tag="JWH23_RS21770"
     CDS             complement(16530..17492)
                     /GO_function="GO:0016002 - sulfite reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asrC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017352819.1"
                     /locus_tag="JWH23_RS21770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfite reductase subunit C"
                     /protein_id="WP_277406185.1"
                     /transl_table=11
                     /translation="MDINTKELKKNAFRITKHRGLTASRVRIPGGYLEARFLSMIQNIA
                     DTYGNGTVHITSRQGFEIPGIPFEDIPKVNALLQPIIEGLGINQEKSGEGYSAAGTRNI
                     TACIGNNVCPYACYNTADFAKKIEKAIFPNNLHFKVALTGCPNDCAKVRMHDFGIMGMT
                     LPHYDADRCVNCGACVKACKRKSTGALETVNCKVTRNHETCIGCGECVLACPTRAWTRS
                     QEKFYRLTLLGRTGKKNPRLGEDFIKWADEDSIFKIILNTYDYVAKYIDTTAPGGKEHI
                     GYIVDRTGFEEFYRWAMKDVTLPEEADVYKPLYWKGVKY"
     gene            complement(17508..18302)
                     /gene="asrB"
                     /locus_tag="JWH23_RS21775"
     CDS             complement(17508..18302)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0016002 - sulfite reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asrB"
                     /gene_functions="other (smcogs) SMCOG1094: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964822.1"
                     /locus_tag="JWH23_RS21775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="WP_277406186.1"
                     /transl_table=11
                     /translation="MNHNEYLPFRSEIKEVIRHTDIEYTFRMAYHGDVKPGQFFEVSIP
                     KFGEAPISVSGIREDSVDLTIRRVGKVTGEIFERYQGDSLFLRGPYGNGFDMENFRGKE
                     LLVIAGGTGLSPVKGVVDYFSSHMEETKGMTLIAGFKTPKDILFLEDFGKWKQAMEVIL
                     TVDCADDDTACQIGLVTQYIPKLKLKNKEETTAIVVGPPAMMRFTTQGLLDIGLKEENI
                     WISHERKMCCGIGKCGHCKIDDTYVCLDGPVFPFTKGRKLLD"
     gene            complement(18292..19310)
                     /gene="asrA"
                     /locus_tag="JWH23_RS21780"
                     /pseudo=""
     CDS             complement(18292..19310)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0016002 - sulfite reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826433.1"
                     /locus_tag="JWH23_RS21780"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGYILNTKEVNEVFQNWKKDSVIYAPVLIKNGGAFSDTDSVHYGE
                     IQSINEIVFDQKSDFSFKEILLPISETLFYFTEDSVKEPEAPVKQAIIFLRSCDLHAVK
                     RLDTMYLHNGAEDPYYKRLRDRVKFILMGCKDSFESCFCVDMGTNRSDDYHASMELWED
                     GVRVDNKNPEWDELFAANCSKQADVTPSYVTETETQVNIPDSLSLDVMKSSMWKQYDSR
                     CINCGRCNFVCPTCTCFTMQDIFYTDNGKVGERRRVWASCMTDGFTDVAGGGSYRQNNG
                     QRMRFKVLHKVYDYKKRNGIPMCVGCGRCDDVPRSTSHFPTVSISWRMP"
     misc_feature    complement(19299..19319)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.65"
                     /tool="antismash"
     gene            complement(19312..20080)
                     /locus_tag="JWH23_RS21785"
                     /pseudo=""
     CDS             complement(19312..20080)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964820.1"
                     /locus_tag="JWH23_RS21785"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="formate/nitrite transporter family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFQEEYSMLAKAAENKSKFLKKNPVGYWVAAMLAGIYVGFGILLI
                     FSAGGMISSQPYVKIIMGASFGIALSLVVMAGAELFTGNNLVMAGGMFSGTVTLGEAVK
                     LWVVCLLGNWAGSILLALIFWGAGLAKGPVGEFMANAAAAKNGNPLHSSLFQSGTLQHL
                     SLSCYLVQLPLQIRERKINHGVLVSFCVYNKRL"
     misc_feature    complement(20174..20190)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.17"
                     /tool="antismash"
ORIGIN
        1 cggaaaagga gaagaggcgc caaagggaaa atacagcgtc aatatcctgg gtgaatacaa
       61 catcggaggt gatggatggg aacaggagcg gatcttaaag aaaatcggat acgaagtagt
      121 ttctgtattt accggagacg gttcctatga aaccttaaaa aattcccatc tggcagattt
      181 aaatctggtc atgtgtcacc ggtccattaa ttacatagcg gaaatgatga agacaaaata
      241 tggagttgac tggctgaaag tgaacttcat cggagtaggg gccacctcaa aatcactgcg
      301 tatgatggct aaatatttta acgatccgga gcttacaaag cggacagagg aagtgattgc
      361 agaggaactg gcagaaatta atgaggatat ggaatattac aagtccaaat taaagggaaa
      421 aacagccgga atctttgtag gaggctcccg ttcccatcac taccagatgc ttttaaggga
      481 ttttggtatg gaaaccacca ttgccggtta tgaatttgcc caccgggatg attacgaagg
      541 acgggatgtt ataccccaga tcaaggcaga tgcagattct aaaaacatag aagaaatttc
      601 ggtggagaga gctgttggat atgatgacag atactctgtg gaagagttaa ccggtctgcg
      661 aaaccagggg gtagagctgg atacctacaa tggaatgata aaagatatga aggaaggcta
      721 tatggtagtg gacgattaca acatgtttga aaccgaccag ctgattgaag agtataagcc
      781 agacattttc ttttccggaa tcaaagataa atacgccata cagcatggag gagtcgtgtc
      841 ccgccagatg cattcctatg attacttccg gaccctatgc aggctttcgt ggagcagtaa
      901 attttggaag agatgtaacc atgagccttt ataccaacgc ctggtcgctg gtaaagccgc
      961 cgtggaagac cataccaatg ttagaaggaa cgttaggagg tgcaggctga tgcttgaatt
     1021 aacaccaaaa gaaattatgg aaaaccgcca catgacaatt aatccctgca aaacctgtga
     1081 gcccgtaggt gctatgttct gcgcactggg tgccgaaaag tgtatgcctc attcccatgg
     1141 atcacaggga tgctgttcct atcacagaac ggtgttaagc cggcatttta aagagcctgc
     1201 cattgcttcc tcgagctcct ttacagaagg tgcctctgta ttcggaggac gcagcaatat
     1261 caccacagct gtgaaaaatg tgtttgacat ctataaaccg gatattatgg caatccatac
     1321 cacctgtctt tctgaaacca ttggtgatga tgttggttcc tacattatgg atatggaagt
     1381 gccggaaggg aaaaccgttc tttatgccag taccccttcc tacgaaggct cccatattca
     1441 ggggttctcc aacatgatga ttggtttcat gcggtatatg acaaagaaga ccgggacccc
     1501 caatggaaaa gtagccatct tcccaggctg ggtcaatccc ggagatgaca gggagttaaa
     1561 gaagattgca gggttaatgg gggcagaata tatttatttt cctgatcatg aggggatact
     1621 ggatcaccct atggatggaa agagtgaata ttttcatgag aatgcaggaa ccaaggccaa
     1681 agatattgcg gcaatgggag attgtgaaaa gctgatctcc atgggaatct actgttctct
     1741 ggaaccgtcg gagtatttaa aaaaggaata taaagttcct ttctccaata taccagttcc
     1801 ggtcggcgtc aagctgactg atcagtacat attggaactt cagaaattta caaagaaaga
     1861 agttccgaaa gagctggaag atgagagggg cagactggtg gacttaatgc tggattccca
     1921 ccagtatgtg tataagaaga aagccgccat ttatggagat cctgatattg tgtatggctt
     1981 caccgcctta tgtctggaac tgggcatgag ccccaaatac atcataaccg gtacaccaaa
     2041 agcacactgg gagaaagaga tcaaagagct gatggatcag tatggtgtag atgaaagcga
     2101 ttatgtggta aaggcagata cggatttgtt ttatctccat cagctgatta aaaatgagtc
     2161 tgtagatctt cttttaggtt ccagctatgg aaagcagatc gcaaaggcag aagacattcc
     2221 tctggtacgt atgggatttc ccgtactgga ccgttatgga aatcccatac aggcgacggt
     2281 aggatatgca ggtgcaatcc ggtatacgga aaagatcgta gacgccatga tggaccggca
     2341 ggaccgggat gcggcagatg aagattttga tgtagttatg taaatcagca gtgaaatgga
     2401 accagggagg gagccgaccc ctccctattt ctgtttaaat ttgcgggtct ttgaaaggag
     2461 tatgtttatg gaaacggaga acagcaaccg gaatatattg gacgcaagga aaagttccat
     2521 tgttgtaaaa gacgggtgca gcggcatggg gggcggaatt tcatgtgaca gcaacagtgt
     2581 atccggttcc gtgtcccagc gggcctgtgt atactgcgga gccagagttg ttttaaatcc
     2641 cattaccgat gcctggcata ttgtacatgg tcccatcgga tgttccagct atacatggga
     2701 tatcagggga tctttaacca gtggggagga tatttacaga aacagctttt ccacggattt
     2761 gcaggaaaca gatattatat ttggaggaat cagaaagctt gaggcagcag tcgatgagct
     2821 gatggagaaa gaagacaatg tgaagctgat ctttatctat gccacctgta ttgttggtgt
     2881 aatcggagac gatgtggaag ccttgtgcaa gatgaaatca gagcagtatc acattccagt
     2941 gattccggta aagtctcccg gtttttccgg gaataaatcc accggctatc ggatggcatg
     3001 tgatgcaatt atgaatgtgc ttctgcccca taaaagtgaa gagaagagaa ctgccattaa
     3061 catattaggg gattttaatc tggcagggga aatgtggatt atcaagaatt attttaagga
     3121 aatcggcatt gatgtgatct caggttttac cggtgatacc tgctatgagg atatgattga
     3181 agcgccgaag gctgctttaa atatagtcca gtgtgcaggt tccagcacct atctggcaaa
     3241 ccgtatggaa gaggaatttg gaattccctt tatcaaggtc agtttttgga atcgaagaca
     3301 ccaccaattc cttaatcagg acggcggaag cacttcaaaa tgaactggca agaagaaaag
     3361 cagtggaatt ttgcaggagg aaggcggaag agctggataa tttcttaggc cgttaccgga
     3421 aaaacctgga ggggaaaaag gctgctatct acgtaggcgg tggatttaaa gcgatctccc
     3481 ttatacgtca gtttcaggca atggggatgg aaacggtggt ggtcggaacc cagacaggga
     3541 aaaaagaaga ttatgaagtg attgagagtc tggtgaaatc cgatactgta attttagacg
     3601 atgccaatcc ggcagagttg gaaaccttta tgaaagaaaa gggagcggat attctggtag
     3661 gaggtgtgaa ggaaagacct cttgcctata agctgggaat tgcattctgt gatcacaatc
     3721 atgagagaaa gtttccttta gccggttttg aaggggggtg gtcaatttca ccatggaaat
     3781 caataaaagc ataaacagtc cggtatgggg ctatatcagc tgacagaaag gcggaatgac
     3841 gatgaagagt gaagtaaaac ctctggtgaa tctaaatatc aatccctgta aaatgtgtat
     3901 gcccatggga agtgcaacgg cattctacgg tctgaagaac tgcatgacca tccttcatgg
     3961 ttcacagggg tgtgctacct acatccggcg tcatatggcg actcactaca atgaaccggt
     4021 ggatattgct tcctcctccc tgactgagca gggaacagtc tatggcggca gtgaaaattt
     4081 aaaaaaggat taaaaaattt aattgagctt taccaccctg aaattatcgg tgtcatgaca
     4141 acatgcctgg cagaaaccat aggagaggat gttccgggga tcttgcagtc ttttctggaa
     4201 gaaaatccag ggtatgagga cattacgctg attccctgcc attctcccgg atatggtgga
     4261 agccagtatg aggggtattt cgcagctctt tctgccattg tctcttccat taagatggag
     4321 acagaaaaga atcatctggt aaatgtaatc acggcaccca taagcccggc agataccaga
     4381 tatttaaaaa ggatgttctc cctctttgga attgatgccg ttctcctacc tgacttatcc
     4441 gataatttag acggagccca cgaccccagt tacaaccgcc ttcctcatga gggaacggat
     4501 ctggcacagt taaagaagat gggtggagca aaatttacat tagaactgac cgcatttcac
     4561 cataagaatt ctgttggcga atatttatct gaaacatatg gagttcctta cgagcggctg
     4621 cccctgccag tgggattaag ggatacggat gcatttttaa atacactgtc ccgtatttcc
     4681 ggtaaagaaa tccctgatga attaaagaaa gagagagagg gcgctattta gatgccatga
     4741 ttgattctca taaataccat tcttcaggaa gagcggctgt attcggggaa ccggatctgg
     4801 tatactccac cgtgcgcatg atgtgtgaat gcggagtcat gccagtagtc tgtgcagcgg
     4861 gatcggcaag tccggaactt gacggactga tcagaccgga gatttctgaa cttgccggtc
     4921 tgtacctggt agatgagtat aaaattgcag ataaagcgga ttttaaggat attgaggaga
     4981 tgattgttac ctataaggcc aatatccttg caggcagttc tgacggaagg cgtattgcag
     5041 aagatttaca ccttccccct ggttcgaaga ggatttccca tacatgatca tgtaggggga
     5101 cagcggctta aaatgctggg ttatgaggga tcggtggaat atatggatga gatcgcaaat
     5161 cagctgatcg acacggttga aactaccttt cgggagactt tatatgatac atattataaa
     5221 gatacagcca ttgatcagaa aaaggaacca caatctgcgg aagagatcat gaagcgcaag
     5281 acaaaggagc acccctgcta taattgccag gctcatcagt tcgccagaat ccatcttccg
     5341 gtagcgcctg cctgcaacgt tcagtgcggc tactgtgtaa gaaaatttga ctgccccaat
     5401 gaaagccgtc cgggagtcac ttcttctatt ctgacgcctg aggaagcact taaccgctat
     5461 ctgtcagtga aggagaaaat gcccaactta accgtagtgg ggatagcagg accaggggaa
     5521 gccctggaaa actgggagaa tgtaagaaag gtatttgaac tcataagaga acaggacccc
     5581 gaagtaacct tctgtctttc caccaatgga ctgaagcttc cccaatatgc gctggaacta
     5641 aaggaactgg gcgtttccca tgtaaccgta accatgaatg cggtggatcc ccatatcagc
     5701 ggacagatgt accagtatat ccggtatatg ggcagggaat acaccggtga cgcagcagga
     5761 gctcttatgg tagcaaacca gattgcggga atcaggcagt taattgccaa tggaatattg
     5821 gtaaaagtta atattgtgac agtgaaggga atcaatgaca gccatgtgcc agaggttgta
     5881 aagactgtca aatcactggg atgctttatt actaatatta tgcagatgat tccggtaaag
     5941 ggcagcgcgc tggaacatgt ggagccggtt tcaaacaaac agttaaatga gatcagaaag
     6001 cagtgtgaag agatcatgcc ccagatgtat cactgtcagc actgccgggc agatgcagtt
     6061 ggaaccctga ccgatgataa atccatacag ttaaaggggt ttgtggcgga ggaaaggcct
     6121 gttatcgttc ctgtttcaaa aaacctccgt tttgcagtag cttccaaaag cggtgtggta
     6181 gttgataccc actttggtca tgcaaaggaa ttttatctgt atgactaccg taacggggaa
     6241 gtccggttcg caggaaaacg aaatgtggag cagtactgtc aggggggaag aagaatgcgg
     6301 ggaaaaagag gataaaatag aacggattat agctgctcta aatggctgca gcggagtcat
     6361 tgccatgcgt atcggtatgg aaccagccaa aaagctgaca aaactgggga ttaagccggt
     6421 gaatacctat gacatgattg aagatgcagt aaaaaaagcg gcggaagcca tgtaagagga
     6481 ggagaaagat ggtacagcca aaatatcatg tatttatctg cacaagctgc agaatcaacg
     6541 ggacacaaaa agggatgtgt taccagaagg gcgcggttga cttaatccag tcttttatgc
     6601 aggagattga agacagggat ctatccggag aggtagttat aaacaatacc gggtgctttg
     6661 gaatctgtga taaaggtcct gttgttgtgg tttatccgga aggagtctgg tatggcaatg
     6721 tatcagagga tgatgtggaa cgcatatttg atgagcattt tgaaggagga aatccagttg
     6781 gtgatttaat gatttaatac ctgtaatgtt gcggaagaaa gcacaggaaa aaggaggggc
     6841 tgctgttgat taaaatcatt gatgccacac tgacgatgct ggaggaatat ccgttaaaaa
     6901 aggagctggt gtacgaattt gcccggacat tagagagtct ttgcatatgg ggagccgtgt
     6961 taacaccaag agtttatcag ctgatgcagg gggatttgcc tcagggaatc acctggtata
     7021 tggaacttcc cagtccatcg gaaagcgtta aatatcccgg gattcatttc tttttttcct
     7081 ctttttgcga tggtacagga aagtttatac gtaatataca agtcaatgat gtgtcagagt
     7141 taagggggct ggaatgtgcc aggccagata accggttaag gcttacgggg ctggacgatt
     7201 tactgattta tgaaagccgt gatatttatg agcgggggat gaagctgtta atccaggcgt
     7261 ctcccatcct gtatccggaa aactcctgct attgtgcttc tgccattgcg gcggattatt
     7321 tacaaaatca ccataacgga acggtgatga ctacctttac cgggatcgga aacaaggcgg
     7381 caacggaaca ggtcattctt gccatgaggg tggtggaacg ttataaacca aacaacagct
     7441 ttgagaaact ggccaacttg cgggatatct ttgaagccat gactaacaca cagatactgg
     7501 gtcatacccc tgttattgga agaaagatct tttatatcga atctggtctg cacgtggatg
     7561 gagttttgaa aaagccatct aattatgaat cctttcctcc cgagcttgtg ggagcatcaa
     7621 gaaaggtgat cctgggaaaa cattcggggg gaacatccat tcggtataag ctgaagcagt
     7681 accagatgga agactgttat ccggtggagc atcttttaga aatggtaaag cgtctcagta
     7741 ttaaaaaggg cggggaagta actgatgaag agtttttaac aatgatgagt gagtgtgata
     7801 agtatgaaaa ggcgaatcaa aatagttgat accactcttc gggatggcga acagtgtccg
     7861 ggtgttgtat tctctccttc tgataaagtg cgtctggctt tgtttttaga cagcatcggt
     7921 gtttctgaaa tcgaggcagg gatttacgat gtgggtacag agggcgtcca ttatatcagc
     7981 gaaattatga aaaagaagaa acaggcagct gtctctctgt ggtctaaatt aaaccctgaa
     8041 aatgtggaag agtcagccag acaaaagccg gatttaattc atatcggcac tcctgtatct
     8101 tatgcgcaga tttataataa attggggaaa aataagaaat ggattgagaa ttcccttcag
     8161 gaatgtatag aagtcgccag gaactataac attcccatta cggtaggtct ggaagatgcg
     8221 tcaagggcag atgtggcttt ccttgtatct ttaattcgtc tgatgcggca aaagggagtg
     8281 gagaccattc gccttgcgga tacggttggg gtgctgtttc ccagacgggc aggggagctg
     8341 gtgcgggaaa taaagcagac aggaattatg atcgaggttc atgaacataa tgactttgga
     8401 atggcagtgg ccaattccat ggttatggcg ctttccgggg ctgatatggt agatgggaca
     8461 ctgcttggaa tcggagagcg tgccggtaac tgcaaccttt acgaatttgt ccatagtgca
     8521 gcccgtaagt ttgatttaaa tgtaagcagt gcagatatta gaaaagggga aaatctctta
     8581 aaatccatat tgaatggagg gaagaagaat gattcggaag atcaaatttg aagatggcag
     8641 aaaatggcgg aaggaatatt aaatctctgc cgggttctct cctgtgaggg gcaggtaatg
     8701 gtaaatggag tttacagttt tgctgcctct gaggtagccg gaaagcttga gggaacagat
     8761 ttgtttttta accagggaat ccgtctggca gccgcagcca ttggtgaaat caatcaggca
     8821 gcaggttgcg gaaccgggga gtccgcccgt ctggcagccc agcttctggc ttactgtgaa
     8881 aggaaatccg catctggaat cagtccggta cttcttgcca gggaactaaa agccgcagca
     8941 tcagcgttgg aagaatttgt tggtaaaaat gcaattcccc tgccagacat aaacacagat
     9001 ttaatagaag gaatcaccaa agaccagaag actgctcaaa tgattgtgga aggatcagag
     9061 gctggggaac tggttgtaaa agaatccatg tccggggaaa ccagttttga aataataaaa
     9121 ggaatgcgtc ttgatggagg tttgacagtg ggagcggaag gaacggtctg ggattccctt
     9181 gttctgattg taaaaacccc catcacttcc ttctcaagca tctatcccct tcttcaaaag
     9241 accggacagc agaatttatt tattctggct gacggaatag agggggaggc cctgactctt
     9301 ttaagtgcaa atgtaaaaca gaaccgaatt catgtaagag ccatgaaggc gccgggaatt
     9361 ggcaggagaa aagaggacct ttttgaggac gctgcggcgt atacagatac catggttttt
     9421 gacggtattt atccttgtca gatggaagaa atttcaccgg ccatgctggg gcgggtaaaa
     9481 gcagtgacag ctgccggttc ttatacaatt ataaaaggaa atgcggataa caaccggata
     9541 tgcaggcgga ttgatggaat taaagagcgg ataggggatc ctaaaaccaa ttattacgat
     9601 aagcagatgc tgcggggcag aattgcagga ttaatggggt gtgcgccagt catctacgcg
     9661 ggtggatata ccacggttca ggcgaaggag gaaaaacgga gaattgagtc tgcattagcc
     9721 tattatcaga ctgtaaagca gtttggagta ctgaaaaaag atgatttaat caattttcaa
     9781 gccggatcag aagcagaaaa gttcctgact gaatccatga agaaatgttt tcagaatgag
     9841 gaaatcagtg catacctgat gattctcatg attcaaaaga tctgtgggct gattgtcatg
     9901 tggctgacaa ccggagcggt catggtgagt accggatatg acagagaaga tttagaactg
     9961 ataaaaagcg gagtggatat agaaaggtta aggagctgat atgaacatca gaaaagtaaa
    10021 gctgaagcct ggatttgcat taatggaact gggaggggaa ttctgtattt tcgatgaaag
    10081 agacagtcag aatggttcct tagacggtct gccatcggta aatgagatct gcatcttctt
    10141 atggaacagc ctggagaggg gagcgggggt ggaggaatta atcactctgt tatctgagga
    10201 aaaaaacatt gatgaagaag atgcaaggct tgagctggag gagtttcttg caaaactgat
    10261 ccatggtaat gtggttgaaa ttaatgggta aaggaggagc aggcaggtgg aaaaaatagc
    10321 ggtgatcagc aaagatcagg aactggttcc ttttttgtat tgcaacctgg tggaaatttt
    10381 tgaaaagaag ggggaacagt ggcaggcggt gcataccgct tccttttctc ccataacagg
    10441 aaatacaatt gaaaagctga gaaaagaaac agaagcaatc atgtctctcg cgaaagatgc
    10501 aaaagcagtt ttatgcagag agctttcagg aatcccattt tccgtattca accaaaaagg
    10561 ttattgcatc ttttgttcag aaaaagcgga ccaggacact ttagacggaa tgataaagga
    10621 tatagaagaa agtgatgaaa aacgaagaag gaaagaagag atgattcaca atgccggacc
    10681 tgtggaaacg cagatacctg gaatctactt tctggatttg gtgcagcttc aaacggagtg
    10741 cccggaggta tcatccaaaa aaggcacttt tgcccttcct ttccaatact ccatttctcg
    10801 aattacagct tgtctgtgcc cacattcccc catggcttga aacagacggt tcctatgaaa
    10861 aaaaagtggg taatggaaat gggaaagtac atataaccat aaccagaaaa cagtgttagc
    10921 aggaggtgga aaaatgcaaa attatatggt tttagactct gggacacttc agactcctta
    10981 tggaattctg gggggagtag attcctttct ctgtcatcca accggtgagt tatcaggagt
    11041 aaccctgtcg gaaaaaaata tgattgttac ccaggcagga gaattggttc cggcttatac
    11101 ggaaaacggc agaagaaaaa agaagccgtc tgtagaattt gacaagcagg gaatggtggt
    11161 tagtgtggcg ttagaagagc agcaggaggt tcttactccc attggagaac ttccggttga
    11221 actgattaag ttttacccca ctggagagct tcaccggata tttatcctgg atggacagat
    11281 aagcggtttc tggacagagg aagatgaacg ggagcttaac atttccctga cctttgatct
    11341 tggttttacg gaatttcagg caatgattaa tggtctttgt ttttacaaaa gcggagatat
    11401 aaaaagtatt acactttttc cggggggaaa ggactgccat acagactccc attggaatgg
    11461 tggaaaccaa ggtgggactt tccttatatg agtcgggaaa cttaaaatca gtagaaccgg
    11521 cagagcctgt cctggtatct actcccattg gtaaaatagc cgcttacgat ccggatcaga
    11581 tcggaatcaa tgctgacagc aattctcttt cgtttacgga aagcggagag ataaaaggtc
    11641 tcatcacctg tgattactcc atctatgttc aaactgagga tggagtgatg agaaagtatt
    11701 ctccccacat aaagccccat cctctctatg atgaggtact cactgtatcc gggattaaag
    11761 tggagttttt tcctgaatcg ggagaagtgt tgattgaaaa tgacagatat gacctggaat
    11821 cctgcggatt taccatagaa ccgtttgcac ggcccggaat gcactgcagc ccttctgact
    11881 gcgcaaactg ttccctgtgc aacaaagcaa tgagggaata aagaacgaaa ctgccaaggc
    11941 tgaatccctt ggcagttttg ttctttgaat gcctaatctg attttgctgc agcatactct
    12001 gtctcactgg aggaagcagt ggcatgcctg ccttttccaa gaacagtcgt atagaaaaag
    12061 gttccaaaac aggcagctgc aaaaattatt ccaattggat aagcaatggt ggtggatatc
    12121 tgaagtcctt cctttgccat caatatgtag gtacaggaga ctgcggacat aaaggttgca
    12181 ggaagcactg taatccagta aaagcgtttt ttacggaaga gataaacgct tgcagtccat
    12241 aatgcgatca tagccagtgt ctggttgctc caggagaaat acctccatac gatctgtaca
    12301 tcaaactgag tcagtacaga gccaactgcc agaagcggta tggtaaggag caggcggttt
    12361 ttcgccggtt tctggtcgag cttaaaccag tctgccagag tcagacgtgc actccggtaa
    12421 gccgtatctg cagatgagat ggggcatgca ataactccaa tcatggcaat aacagcacca
    12481 accggtccaa gaagctgaaa gcagatttca taaacaacac tggactgccc atttccaaga
    12541 gccgttaaaa gaccgccagt tccatcgtaa aatgcaactc ccgctgcagc ccagatgagt
    12601 gcaatcacac cttctgcgac catggctcca taaaatacct tccggccgtc tgcctcattg
    12661 gtaagacagc gggccatcaa aggagactgt gtggcatgaa atcccgatat ggcgccgcag
    12721 gcaacagaga taaacatggt gggccagata ggtgtcccgg ctgggtgaag attggccagg
    12781 gtgatttccg gaatgtgata tcctttaaaa agaatggctc cgcctacacc cagtgccatt
    12841 actatcaggc agataccaaa gaacggatag atttttccaa tcaccttatc aatagggaca
    12901 aaggtggcaa taaaatagta gaccagtaca acaacaagcc agaatctgga atccagtaca
    12961 tgaggtgtca gcatcgctaa gagatttgct ggcccggtag aaaaggtcac ccctaccaat
    13021 actagaagaa ttactgaaaa tacccgcatg atctgcttca tggcagtgcc aagataggaa
    13081 ccggtcagtt ctgatacact ggcgccctta tgccgcatgg acatcatgcc tatcatataa
    13141 tcgtgtacac caccccctaa aagagtaccg aaggtgatcc ataaaaatac actgggtccc
    13201 cagcaggctc cggcaagtgc accaaagatg ggccccagtc ctgcaatatt taacagctgg
    13261 attaaaaaaa tccgtggggt ccccatggga acgtagtcgt taccatctgt catggaaaga
    13321 gctggtgtta ttctgtcatc aggtccgaaa actttctctg tgattttgct gtagaagaaa
    13381 aatcctgcta tcagcagtaa cagacataaa aagaaactta tcatttgcct acctcctatt
    13441 attaaacttc tcattaccac taattataat gaaatgtgca caataataaa tggaaaagga
    13501 ataaaaagca ggtatttatg cataaacagc agttaaacac caatgatctg cttaaactgt
    13561 ttgatctttt ctcttcctac gggcagaatc tctttttcaa atccctgcat tttaactgcc
    13621 atactgttat ttgcccatgg aaacatttca gatatgtaat ttaagttgat cagataactt
    13681 ttgtgaatcc ggtaaaatcc acaggaggaa aggcgtttct catattcacc taacagctgg
    13741 ttctccgtat aatccctgga gacggtatga ataatgcagc ttcggcagca ggtttctatg
    13801 tatacgatct gtctgatatc aagcagtata atggttctgt tgacattaat gggaaacttg
    13861 tgtgctggga ggcataaatc attttcaaag aatgatccaa atcctgtctg catttttcca
    13921 gaatacagcg taaccgttcc ggatcaaagg gttttaaaat atagttattt acacccagtt
    13981 caaatgcctg gactgcatat tgggaaaatg cggttgcaaa gataatctgg gcatgaggca
    14041 tcattctcct tgctgcagcc gccagtgtgg ttccatccat atcatttaaa ctgatatcca
    14101 gaaagagaat atcaaaataa tgatggccaa ataattcaag ggctgcatta ccactgtctc
    14161 cttccatgat ttcgctttca ggcaggaagg ataaaatctg gtggatcagt tctttacgtg
    14221 cagggcgttc atcatcaatc acagcaattt tcataattca atcccccttc atattttgat
    14281 tctggagaga ccggaatggt aaaaaagatg tcagaaccgg aatcagaacg ttcaatatga
    14341 agcccgtgct cctgtccata aatgcttttt aaccgtttat ggacattttc cagtcctact
    14401 cccccgtaag gttctccttc atacagatgc tttatgattg tttctggaat tcccggtcct
    14461 ttatcggaaa cggagatcct tataaatgct ccctccatac aggcgcagat ggaaacatag
    14521 cgatttgcgc ttttatcaaa accatagcgg attgcatttt ccaccagggg ctgaaggatg
    14581 aacgccggaa tcaggcagtt tgttttttcg tccgttatta tttcgatatg aagcttttct
    14641 tcaaatcttg ctttttccaa ttcccagata tctggtaata tgcataagct ctgtcttaag
    14701 tgtgtgcatg taggaattat gctccagtgt ctgtctgtaa taggaagaca aagtcagaag
    14761 cagttctctt gctctgtccg gattgtcccg gcagacgtag gagatggtgt taagagaatt
    14821 ataaaggaaa tggggattga cctgagactg cagagctttt aattctgcct tacgccgtaa
    14881 ttttttctgg tattccagtt cagacagctc aaactgagtg gaaaaaagcc ttgcaagttc
    14941 cgaggcaaaa tttaaatcag actgggactg ttgccagtgc ttttttacta tcatggtcag
    15001 acttcccata acctgattca tttcaataat gggagcagcc acaataatat ggtttttcaa
    15061 aatagaataa agtggttccc cttttctggc ataatgaaaa atggttggtt tcctgtcgtg
    15121 gatggacgcc aggattggga gaagaaccgt atcttcattc atatccttca gttccggatt
    15181 ggccttcttg cttattattt tttcggtatc tgttataagt attgcggcgc agctggtgga
    15241 ctgaaaaata atttcagcag cagcctgcat atctttgata ctgtataaac ctttgcgaag
    15301 atggggaaga ctttgttcca cgattccaag agccagccgc atccgttcag caaactgact
    15361 gtcttcctcc aaaaacacca tattaaaagt gcccagaaat actaacattc caagtgcgtt
    15421 catggtcatc atggggagtg agatcagctt tacaagctgg actgctgcag aaaagggccg
    15481 tgaaataagc agaataatca tcatctgaag aaattctaca aaaaccgtga gaataaaaac
    15541 actggagctg cccatttttc cggacttaaa ccggctggag aacaaggagc ctgcaagccc
    15601 ctcaaccaga gtggagacag cacaggctgc cgcggtaaat ccaccgatgt caaacagata
    15661 acggtgaatt cctccaatta aggctgcacc gattcccaca tatgggccgc ctaaaaggcc
    15721 ggcagccagc actccgatta cccgggtgtt gacaattgct ccctgggtgc gaacccctgt
    15781 gtaggttgat ataatgctga ttaaaccgaa taagatggct agcatgattc cgcagccggc
    15841 ggaatgcata tcataaagga gcatgctcct tacaatgggg attttggtaa gaagggtagc
    15901 gatcagaacc agaagaccga tatttaaaag aagactgaaa aacatagcac ttgacataag
    15961 tttccccctt atactggctg ttaaaattct gttataatta taacagatat ttgaaagaaa
    16021 tacagtggga gtcatggaag atagaaagta acttgcattt atagtcagga atcatatata
    16081 atttaacatc aggaaccgat gcaggcaaaa tagagtttac tggaggaaaa tgatgaaact
    16141 atttataaag tatatttcaa ttgtattagc catttatctg atgtcgttta tatttaactc
    16201 ggtttatata ggaagtttag gcgcactgct tattatgggc atggtgcttt tgcttgtgaa
    16261 cttaataata aaaccgtttc ttatgctcat taccttaccc gccaatttgc tgacgttagg
    16321 cttattcagc tttattgtaa atgcatggac gatcatgatc gccgattccc ttgttgaaaa
    16381 tataagtatg ggcggctttc ttaattcgct gattgcagct ttcataatta caattttaaa
    16441 taatctgttt caggataaaa attaggcaac aggataaaag catgaccgga atccacgagt
    16501 atgggttccg gtcatgcttt tattgttttc taatatttga cgcccttcca gtaaagcggt
    16561 ttataaacat ctgcttcttc cggcaatgtc acatccttca tagcccaacg gtagaattcc
    16621 tcaaatccgg tccggtccac aatatatccg atatgttcct ttccgccggg agctgtggta
    16681 tcaatgtatt ttgccacata atcatatgtg tttaaaataa ttttaaagat actgtcctca
    16741 tctgcccatt tgataaaatc ctcgcccaaa cggggatttt tctttccggt acgtccaagc
    16801 agggtaaggc ggtaaaactt ctcctgactt ctggtccatg ctctggtggg gcaggcaagt
    16861 acacattccc cgcagccgat gcaggtttca tgattgcggg ttaccttaca gtttactgtt
    16921 tccagagccc cggtagattt ccgcttgcat gctttcacac aggcgccgca gttgacacag
    16981 cggtctgcat cgtagtgggg gagtgtcatt cccataatac cgaaatcatg catccgcacc
    17041 ttggcacagt cgttggggca tccggttaat gctaccttaa aatggagatt gtttgggaaa
    17101 atggctttct ctattttctt tgcaaaatcc gccgtattat agcaggcata agggcagacg
    17161 ttgttgccga tgcatgcggt tatatttctt gttccggcag cgctgtagcc ctctcccgat
    17221 ttttcctggt taatacccag tccttcaata atcggctgca ggagggcatt gacctttgga
    17281 atatcctcaa atgggattcc agggatctca aaaccctgtc tggaggtaat gtgtacagtg
    17341 ccattgccgt aagtatcggc aatattctga atcatagata aaaatctggc ttccagataa
    17401 ccgcctggaa tccggacgcg ggaggctgta agacctctgt gtttcgtaat ccggaaagca
    17461 ttttttttca gttcctttgt gttaatatcc attagacgtt cccctttcta atccaagagt
    17521 ttgcggcctt tggtaaatgg aaagacaggt ccgtccaggc acacataggt gtcatcaatt
    17581 ttacagtgtc cgcatttgcc gattccgcag cacattttcc gttcatggga tatccagata
    17641 ttttcttcct tcagtccaat atcaagaagt ccctgagtgg taaaccgcat catggctgga
    17701 ggccccacca caattgcagt ggtttcttct ttgtttttca gctttaattt tggtatatac
    17761 tgggtgacca gtccgatctg gcaggccgtg tcatcatctg cacaatcgac agtcagaatg
    17821 acttccatcg cctgcttcca tttgccgaag tcctccagaa agagaatgtc tttgggtgtc
    17881 ttaaatccgg cgatgagtgt catgcccttt gtttcttcca tatgactgga aaaataatca
    17941 actacaccct ttacaggaga aagtccggtt ccgcctgcta tgaccagaag ctcttttccc
    18001 cggaaattct ccatatcaaa tccattgccg taaggtcctc ttaaaaacag gctgtctccc
    18061 tgataacgtt caaagatttc tcctgtcacc ttacctaccc ggcgaatggt taaatccacg
    18121 gaatcctccc ggatgccact gacagagatg ggcgcttctc cgaactttgg aatggaaacc
    18181 tcgaaaaact gacccggttt tacgtctcca tgataagcca tgcggaatgt gtattcaatg
    18241 tccgtatgac ggattacctc cttgatttca gaacggaaag gaagatattc attatgattc
    18301 atttccattt acctccttca tggcatcctc cagctgattg atacagtggg aaaatgagat
    18361 gtactccggg gcacgtcgtc gcaccggccg cagcctacgc acataggaat accattgcgc
    18421 ttcttgtagt catacacttt gtgaagaacc ttaaagcgca tgcgctgtcc gttattctgg
    18481 cgatagcttc cgcctcctgc tacatcagta aaaccatctg tcatacagga agcccatact
    18541 ctgcggcgtt ctccgacttt accgttatcg gtatagaaaa tatcctgcat ggtaaaacag
    18601 gtacaggttg gacatacaaa attacaccgt ccgcagttga tgcagcggct gtcatattgt
    18661 ttccacatgg atgacttcat aacatctaag gaaagagaat cagggatgtt gacctgtgtc
    18721 tcagtctcgg ttacgtaaga aggggttaca tctgcctgtt ttgaacagtt ggcagcaaat
    18781 aactcatccc attccggatt tttattatca actcttactc cgtcttccca cagttccatg
    18841 gaagcatggt agtcgtctga acgattggta cccatatcaa cacagaaaca gctctcaaag
    18901 gagtctttac accccataag tatgaatttt acccggtccc gaagacgttt ataataaggg
    18961 tcttcagccc cgttatggag atacatggta tctaagcgtt tcactgcgtg aagatcgcag
    19021 ctgcgcagaa aaataattgc ctgcttcaca ggggcttccg gttcctttac cgaatcttcc
    19081 gtaaaataaa ataaggtctc cgagatggga agaagtattt ctttaaaaga aaagtctgac
    19141 ttctggtcaa atacgatttc gttaatggac tggatttctc catagtgaac agagtccgta
    19201 tcggaaaaag cacctccatt ttttatcagc acgggagcat agatgacgga atcctttttc
    19261 cagttttgaa atacttcatt aacctctttt gtattaagga tatatcccat gctaagcctc
    19321 cttttttccg gaaatcagat aatagggaac agcagtaaat aaaattccgc ctgccatatt
    19381 gccaagagtt acaaccagaa tattataaaa atatccggaa agggaaacag ccgcaccgtt
    19441 agggcttaag actcctaccg tgagcagagt catgtttgca atgctgtgct catagccgct
    19501 tgttataaac gcaaaaagac accagaacac catgattaat tttccgctct ctgatttgca
    19561 gcggaagctg caccagatag caagacaaac taagatgttg caaagtaccg ctctgaaaaa
    19621 gagaggaatg aaggggattg ccattttttg cagcagcagc atttgccatg aattccccta
    19681 ctggtccctt tgccagcccg gcaccccaga aaataagagc aaggagaatg gaaccggccc
    19741 agtttccaag taaacagacc acccaaagtt tgacagcctc cccaagtgtg acggtaccgc
    19801 tgaacattcc gcctgccatt actaaattat tgccggtaaa cagttctgct cctgccatta
    19861 caacaagact gagggcgatg ccaaaggatg cacccataat aatcttgacg tagggctgac
    19921 tggaaatcat ccctccggca gagaagatca atagaattcc aaaacccaca taaatacctg
    19981 ccagcatggc cgcaacccag tatccaacag gattcttttt cagaaacttt gatttgtttt
    20041 ctgccgcttt tgctaacatt gaatattctt cctgaaacat aaacaacctc ctttgtttat
    20101 tttttatcaa actaaccaca aaacaagtat aatcaataaa taaaaaaaat accgtgatct
    20161 aaatcacggt gacaggttat tttaatacaa gtctcgttga ccttatggat acttaatgtt
    20221 aaaattaatc tgattttaat taaaattaag tatagaagca ggtagttaaa tggagaagaa
    20281 agttacaatg c
//
