LOCUS       NZ_CP070896            20546 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Lacrimispora xylanisolvens strain ASCUSBR21 chromosome, complete
            genome.
ACCESSION   NZ_CP070896
VERSION     NZ_CP070896.1
KEYWORDS    RefSeq.
SOURCE      Lacrimispora xylanisolvens
  ORGANISM  Lacrimispora xylanisolvens
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Lacrimispora.
REFERENCE   1  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Hungatella xylanolytica ASCUSBR21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) R&D, Ascus Biosciences, 6450 Lusk Blvd E209,
            San Diego, CA 92121, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAY-2019
            Assembly Method                   :: Canu v. 2.1.1
            Assembly Name :: Hungatella xylanolytica ASCUSBR21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017084465.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,443
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 686
            CDSs (without protein)            :: 686
            Pseudo Genes (ambiguous residues) :: 0 of 686
            Pseudo Genes (frameshifted)       :: 599 of 686
            Pseudo Genes (incomplete)         :: 95 of 686
            Pseudo Genes (internal stop)      :: 24 of 686
            Pseudo Genes (multiple problems)  :: 30 of 686
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:11:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1302361
            Orig. end                         :: 1322907
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20546
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="4"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20546
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20546
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10546](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10546)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            249..887
                     /locus_tag="JWH23_RS07350"
     CDS             249..887
                     /EC_number="2.7.7.65"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /locus_tag="JWH23_RS07350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diguanylate cyclase"
                     /protein_id="WP_277408602.1"
                     /transl_table=11
                     /translation="MHWFQKKENIISDDKQTPGTEQGSDSSVKLERDACLHHLTHALSS
                     NERGVVLKLYIENFKQLNQLLGYEYCENLLSQILAYLKESCGNNVYHYIGVEYMIFLDH
                     YTQGQAMNLADEIAQRFDHAWKIGSTDCICSVQMGLCAYPGHAETTEQMLKCLDLAVTR
                     AGEGGPNQAVMFDSTLQNQLRRRQTIALYLKTALEKMKWRFFTVLPLAL"
     gene            851..1599
                     /locus_tag="JWH23_RS07355"
                     /pseudo=""
     CDS             851..1599
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /locus_tag="JWH23_RS07355"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="EAL domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEILYRPSFSVVTGTFLRAELSMRIFIKGLGLIGAGEFMTVAEDS
                     GQIRALLYYALEKVCQCIRQALDDGCEFESIALPVSPVLFLQEDFIDEVARVMEAYEVP
                     QGKLALELRESALTMGYLNFNIILQKLSDMGVELILNEFGSGYSSVSASLDLPVHTVKL
                     ERLFIWQLETNENPDILYRD"
     gene            complement(1657..2106)
                     /locus_tag="JWH23_RS07365"
     CDS             complement(1657..2106)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723102.1"
                     /locus_tag="JWH23_RS07365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CBS domain-containing protein"
                     /protein_id="WP_104436368.1"
                     /transl_table=11
                     /translation="MNILFFLTPKNEVAYIYEDDSLRQALEKMEYHKYSAIPVINRNGK
                     YVGTITEGDMLWGIKNKFNLSLKEAENVTVAAIDRRSDNRPVFADSTMEDLVDMALNQN
                     FVPVVDDQKNFIGLVTRKDIIRYFYKKSQEVKKSDEIKVRTIVSN"
     gene            complement(2217..2717)
                     /locus_tag="JWH23_RS07370"
     CDS             complement(2217..2717)
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /locus_tag="JWH23_RS07370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_349772939.1"
                     /transl_table=11
                     /translation="MIQPKPQPKKKHRQQKPQQTNAAKSLVLNFNDSTRMTWPVRGNVI
                     LDYSMDKTIYFPTLDQYKCNPGVVIQGDVSTPVVAPANAKVQEIGSNEEIGNYVVLNMG
                     NKYTAVCGQLKELKVTANEYVKEGQVLGYIAEPTKYYSVEGANLFFEVTHEDKAIDPLD
                     HMQ"
     gene            complement(2632..2970)
                     /locus_tag="JWH23_RS31125"
     CDS             complement(2632..2970)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS31125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_349772940.1"
                     /transl_table=11
                     /translation="MKEKISQLFKDKVFLVLLVLGLLTIVAAAGVITVQRGNGGGESPY
                     LKVPDQSNMIVEESVPQDTQVAVAGDSNATKNAEEEMKAVDSAKATAEKETQAAKAAAD
                     KCRQVTGT"
     gene            complement(3011..3931)
                     /locus_tag="JWH23_RS07375"
     CDS             complement(3011..3931)
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602937.1"
                     /locus_tag="JWH23_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="WP_277408605.1"
                     /transl_table=11
                     /translation="MGKKILMACILAFLFPYIITLAWTGKIEEKKEMPITLSGKRVILD
                     RKGGETSMDVEEYLPGVVAKQMPADYGKEALRAQAIIARTYIYGKMNDQKEVKESDLHM
                     DYLEQKQMEKMWGSEAFVTSYQAIEDAVRSTTGTVITCNDKLIEPLFHRASNGMTRAGD
                     SNHPYLQPVECKKDVEAEGFLTILQFSKEDFADKINKISGDGSVKADQIPQSIQIVQRD
                     DSGYVVQIQIGTHTLTGDQVQYALSLPSPSFEFEDYEGGVRAVCRGIGHGYGFSQYGAK
                     CKSEEGWTAEKILAYFYKNIVLKSE"
     gene            complement(4033..6487)
                     /locus_tag="JWH23_RS07380"
                     /pseudo=""
     CDS             complement(4033..6487)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564757.1"
                     /locus_tag="JWH23_RS07380"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSMGFDKETFKKSVLFNMKNVFRKTVDEATKEQMYQAVAYAVKDV
                     IIDEWIATHKEYEKQDVKNPVLPVHGVFNGSCSWQQHHKFHGTAGCKGSVRGTWV"
     gene            complement(6509..9624)
                     /gene="ileS"
                     /locus_tag="JWH23_RS07385"
                     /pseudo=""
     CDS             complement(6509..9624)
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ileS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602934.1"
                     /locus_tag="JWH23_RS07385"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="isoleucine--tRNA ligase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYRKVPTDLNFVEREKEIEKFWEEHDVFKKSMDNRKEAETYTFYD
                     GPPTANGKPHIGHVLTRVIKDMIPRYRTMKGYDVPRKAGWDTHGLPVELEVEKKLGLDG
                     KEQIEEYGLEPFIKYCKESVWQYKGMWEDFSKTVGFWADMDDPYVTYDNNFIESEWWAL
                     KQIWEKGLLYKGFKIVPYCPRCGTPLSSHEVAQGYKDVKERSAIVRFKVKKRGCIYPCM
                     DHNSMDPAFQRWTLCESV"
     misc_feature    9644..9657
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.13"
                     /tool="antismash"
     gene            complement(10001..10546)
                     /locus_tag="JWH23_RS07390"
     CDS             complement(10001..10546)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="JWH23_RS07390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_277408606.1"
                     /sec_met_domain="AgrB (E-value: 1.7e-33, bitscore: 114.1,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRWEETFVEKLVSNEIINEKEADLYQFGIECLVLKLIHCISYLC
                     IAACFQMVPQLIVIGCVLIPLRRNAGGYHAKTKMGCYIFSCCYIAIILFLSNAQINQVL
                     WWGALALSDAVLFFKSPADNENKRLDEKEVVHYRRKARLILILANAGCLVLTAFHHCYA
                     GNLFICGITAAAFLLVLS"
     gene            complement(10793..11449)
                     /locus_tag="JWH23_RS07400"
     CDS             complement(10793..11449)
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414054.1"
                     /locus_tag="JWH23_RS07400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="WP_277408608.1"
                     /transl_table=11
                     /translation="MNKRESIVRRIAKELKDGDVVNLGVGMPTMVANYIPDDVHIMLQA
                     ENGILGVGPNADQEDKTLDCIDAGGNYVTTVKGASYFDSSFSFCIIRGGHVDVTVLGAL
                     EVDEKGNVANWMIPGKKVPGMGGAMDLVVGAKKMILAMEHVSKEGAPKILKKCRLPLTA
                     VNVVKTIVTDMAVIDVVPDKGLVLKEIAPGLTVEDVQNATEAHLNVAPDLKVIHV"
     gene            complement(11453..12109)
                     /locus_tag="JWH23_RS07405"
     CDS             complement(11453..12109)
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414055.1"
                     /locus_tag="JWH23_RS07405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CoA transferase subunit A"
                     /protein_id="WP_277408609.1"
                     /transl_table=11
                     /translation="MNKLISAGEAVKKVKDGDAIMIGGFLAGGHPENLVNALLNTSTAS
                     DLAIISNDTGTTELSIYQLIKSGRVKRIFASYIGSNPETGRLLMTGEAKVQLFPQGTLA
                     EKIRAGGAGLGGVLTPVGLGTVVEEGKRKIEIGEKNYLLEMPLKANVAFIKAQKADETG
                     NLIINGSSRNFNVVMATAADYVVAEVDEYVKAGEIDPNDVTVPGIFIDNIVKVGQ"
     gene            complement(12128..12547)
                     /locus_tag="JWH23_RS07410"
     CDS             complement(12128..12547)
                     /EC_number="4.1.1.4"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF002614.0"
                     /locus_tag="JWH23_RS07410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetoacetate decarboxylase"
                     /protein_id="WP_277408610.1"
                     /transl_table=11
                     /translation="MKSGDFPKKWGQPHLRVEDTETLTGTLIYNNILVAMGTMPFKYNI
                     LDKKAVAKNLGKMQVNLKLIPDADGSPKIAQLVAYNLEDIQVKEAWSGPARLHLIPHVN
                     APVADLPVKACMGGTHIISDLTLPYGRVIHDYLSE"
     gene            complement(12501..12872)
                     /locus_tag="JWH23_RS07415"
     CDS             complement(12501..12872)
                     /EC_number="4.1.1.4"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF002614.0"
                     /locus_tag="JWH23_RS07415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetoacetate decarboxylase"
                     /protein_id="WP_277408611.1"
                     /transl_table=11
                     /translation="MKSETIISLQSMPAASASYGRPPCRFINREYFTVSYESDPDLIKA
                     AVPEPLEPDGSNLVLYEWIKMPDSSGLGTYEESGIVIPCTYHGEACNFTAQMYLNNGPA
                     ILAGREIWGFPKEVGPAPP"
     gene            complement(12902..14085)
                     /locus_tag="JWH23_RS07420"
                     /pseudo=""
     CDS             complement(12902..14085)
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124: acetyl-CoA acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908915.1"
                     /locus_tag="JWH23_RS07420"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="acetyl-CoA C-acetyltransferase"
                     /pseudo=""
                     /sec_met_domain="ketoacyl-synt (E-value: 5e-05, bitscore:
                     21.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIALISPVRTAVGSFNGTLSTLNASELGTAVIQSCLEQSKLES
                     SLIDAVYLGNVLQAGIGQSPARQAAVNAGIPVEIPASTINTVCGSGLHTVALAYDSILA
                     EQNSIVLAGGMESMSNAPYLLKKARNGYRMGNGELVDSLVADALTCAINHYHMGITAEN
                     VAEKYNVTRKEQDEFSYNSHMKAAIAKNAKIFDSQIVPITVKKKKEEICFSEDEHIKPD
                     TTLEKLSGLRTVFKTDGTVTAGNASPISDGAAAMLVASEDKCREYDLKPMAYIKGYSLV
                     GVDPAYMGIGPVKAVSTLLKNRD"
     gene            complement(14318..15340)
                     /locus_tag="JWH23_RS07425"
     CDS             complement(14318..15340)
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="JWH23_RS07425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_277408612.1"
                     /transl_table=11
                     /translation="MILILTVCETAGVVMLEYILLKFQIHGIQPVMIKSMELTFSKLVV
                     LIVYYLIITRLWREDFAVKYTITQCLIQVIIILFSMTNIAVIIVVIPKITNISEYILIL
                     INMGCILFADLYFLYLARFVEENNQLKVKLKLLEQQSLLQYEFYEDQKEKYNESIIILH
                     DVYKRLNLIEEMYRTNEHKAALNYAKEIGRLLIPLSLEDYTNNPILNVILNDKKRIAIF
                     HKIKFNLEIGVVDLSFMEPIEVTTIFGNLLDNAIDACDQVPQDRFIKVKLDKYNDFTVI
                     NITNSTAPIERWHRGKPVSKKGKNHGIGLLNVENIIKKYNGSMVLEEENRIFSCKIIFN
                     "
     gene            complement(15636..16379)
                     /locus_tag="JWH23_RS07430"
     CDS             complement(15636..16379)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="JWH23_RS07430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_277408613.1"
                     /transl_table=11
                     /translation="MLLIAICDDNKSDSVRIAKFVKGYMKSVSIPFQIITFKSGEELLE
                     FHGKFDLIFLDIALTGMNGIQIGKTIWEMNRNVKVIYTTSYKQFCRQAVNYVHAFAFLE
                     KPVTKINVERQLQEIIQYIQEESGKLEIVNFEILEVTEAGELESRIKSFEIKDIFYFEY
                     INRKIKIKLENEEYFFIDKMKNLTERMQKHNFEACHQCILVNLRHIKNIKGYEVLLNNN
                     DKLPVSQKKSADFRKKLNNFIQSNV"
     misc_feature    16988..17001
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.67"
                     /tool="antismash"
     misc_feature    17115..17129
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.11"
                     /tool="antismash"
     gene            17259..18722
                     /locus_tag="JWH23_RS07435"
     CDS             17259..18722
                     /EC_number="6.3.4.21"
                     /GO_function="GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602928.1"
                     /locus_tag="JWH23_RS07435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="WP_277408614.1"
                     /transl_table=11
                     /translation="MSQRNLTLLTDLYELTMMQGYFKEKDANETVIFDAFYRSNPGGNG
                     YAICAGLEQVIEYIKELEFNSEDVDYLRSTGLFEEDFLEYLLHFRFSGDIYAIPEGTVI
                     FPREPLVKVIAPIMEAQLVETALLTIINHQSLIATKTARVVFAAEGDGVMEFGLRRAQG
                     PDAGIYGARAAMIGGCVGTSNVLAGKMFNVPVKGTHAHSWIMSFPDELSAFRSYAKLYP
                     KACILLVDTYDTLKSGVPNAIKVFKEMKEANIPLTSYGIRLDSGDLAYLSKKAKKMLTE
                     AGFPDAIISASNDLDETLINSLKIQGATINSWGVGTNLITSKDCPSFGGVYKLAAVLDR
                     KTGEFNPKIKLSENAEKITNPGNKGIKRIYSRETGKIIADLICLEGEVYDENHSLLLFD
                     PIETWKKTHLAPNTYTMRDLLIPVFQKGKCVYESPAVMDIQAYCKKELDTLWDESRRLV
                     NPHEVHVDLSKPLWNMKNQLLDNYHFQES"
     gene            complement(18956..19138)
                     /locus_tag="JWH23_RS07440"
     CDS             complement(18956..19138)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004826.1"
                     /locus_tag="JWH23_RS07440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6472 family protein"
                     /protein_id="WP_104436381.1"
                     /transl_table=11
                     /translation="MAKKSSCEYCMYYQYNEDYEYYECGINLDEDEMMRFLSESHQSCP
                     HFKYGDDYSIVKKQM"
     gene            complement(19234..20043)
                     /locus_tag="JWH23_RS07445"
     CDS             complement(19234..20043)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /locus_tag="JWH23_RS07445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyltransferase"
                     /protein_id="WP_277408615.1"
                     /transl_table=11
                     /translation="MLSGLFILDHGTENILAFYKKRFCTIVIPFGFYAGIYYYYYNVYL
                     GQEKLGFFAHIKAWLFGWLTEATPMAPHMWFMYVILGLYLCAPFLSRMLKAMTDHELRI
                     FLAMILMIQTLFVYLQPLGFLPGEFFQYMIFKGWLIYFVLGYILKRLYQNTRYFPFLAA
                     GLAGFALTMVQKFYTPGFTPGIHDLAPTMIAMASAVFLFFERYGTVSFPVFTNACGLIS
                     RHSYGMYLIHYLVLGQFSAAIIEKTWIRHFYIPKIICLTALTFSFLW"
     gene            complement(20055..20207)
                     /locus_tag="JWH23_RS07450"
     CDS             complement(20055..20207)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS07450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277408616.1"
                     /transl_table=11
                     /translation="MERKNREIQYDYLRVIAVIAIIMVHSIPAQAANDTQQLLVQCLCL
                     CFCLL"
     misc_feature    complement(20279..20292)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
     tRNA            20354..20425
                     /anticodon=(pos:1322748..1322750,aa:Glu,seq:ctc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="JWH23_RS07455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Glu"
     gene            20354..20425
                     /locus_tag="JWH23_RS07455"
ORIGIN
        1 cacctctgtc cgtctgtttc caggaagagg gtttctcccc ggtatccgga ctgtttctgt
       61 ccatattgaa ttcattaatt ttcacctctg ttgtttgtct ttccaacgct gtattaatat
      121 gcatatttcc tattattctc acatttgaca gaagtaatgc cttttttccc gtttccaatt
      181 atttcttgct ttcctctctt ttctatacta aaataagaac agacaaccaa gaacaggagg
      241 caatcccaat gcactggttt caaaaaaaag agaatattat ttctgacgat aaacagacac
      301 caggaaccga acagggcagt gattcttcgg ttaaactgga acgtgatgcc tgcttacatc
      361 atcttaccca cgcactcagt tccaatgaac ggggcgttgt tttaaaacta tacatagaga
      421 attttaagca gctcaatcaa ttattgggtt acgaatattg tgaaaatctg ctgagtcaga
      481 ttcttgccta tttaaaggaa tcatgcggaa ataatgtcta tcattatatc ggcgtggaat
      541 atatgatctt tttggatcat tatacccagg gacaggctat gaatcttgca gacgaaatcg
      601 cccagagatt tgaccatgcc tggaagattg gcagcacaga ctgcatctgt tctgtacaga
      661 tgggactttg tgcctatccc ggccatgcag aaaccacgga gcagatgtta aaatgcctgg
      721 atctggcagt gaccagagct ggtgagggcg gccccaatca ggcagttatg tttgacagta
      781 ctctgcaaaa ccagcttagg cggcggcaga ccatcgctct ttatttaaag acggctctgg
      841 aaaagatgaa gtggagattc tttaccgtcc ttcctttagc gttgtaacag gaacattttt
      901 aagagcagaa ttatccatgc ggatatttat aaaaggtctg ggattaatcg gagctggaga
      961 attcatgact gtggcagagg attccgggca gatccgtgca ttgctttatt atgctctgga
     1021 aaaggtctgc cagtgtatca gacaggcact cgatgatggc tgtgaatttg aatccatagc
     1081 acttcctgtc tctcctgtgc tatttttgca ggaggacttt attgatgaag ttgcacgggt
     1141 tatggaggca tatgaggtgc cacagggtaa gcttgcgctg gaactgagag aaagtgcact
     1201 gaccatggga tatttaaact ttaatattat tcttcagaag ctttcagata tgggagtgga
     1261 actgatactc aatgaattcg gctctggtta ttccagtgtt tcggccagcc tggatcttcc
     1321 cgttcacact gtaaagttag aacggctctt tatctggcag cttgagacca atgaaaatcc
     1381 agatatatta tacagggatt aattcgcatg gcaaaggatc ttcacctgtc cataatcgca
     1441 gaaggcgtgg aaacggaaaa tcaggtgact gttttaacag aggcaggctg cagctatcag
     1501 cagggatttt attattccac tcccttaaag caggagaatc tgttgaggat tttaggaaag
     1561 aaccgggatg aatcagctcc ttatctgtct gaggattaaa aatatgggat gaaaaaagcg
     1621 ctttcggcta aaaaccgaaa acgcttttct atctgcttaa ttacttacaa ttgtcctgac
     1681 cttgatctcg tcggactttt ttacttcctg ggacttcttg taaaagtagc gtatgatatc
     1741 cttacgggtt actaacccga tgaaattctt ctgatcatcc accaccggta caaaattctg
     1801 gttcaaagcc atatcaacca ggtcttccat tgtggaatct gcaaaaacgg ggcggttatc
     1861 cgatcttctg tcaatggcgg ctaccgtcac attctccgct tccttcagac ttaaattgaa
     1921 tttattcttt attccccaga gcatgtcccc ttctgttatt gttccaacat atttgccgtt
     1981 cctgttgatg acaggtatgg cggagtactt gtgatactcc atcttctcca gcgcctgacg
     2041 cagagaatca tcttcataaa tataagccac ttcattctta ggtgttaaaa aaaacaatat
     2101 gttcatagcc acactccccg tttgatgtac ggacttagta tagcagatga gtcttaatca
     2161 cgtatgaaca aattattatt tttttataac tttttcctaa tatttcctga gcatgattat
     2221 tgcatatggt caagaggatc aatggctttg tcttcatggg tcacttcaaa gaataggttt
     2281 gcgccttcta cggaataata ctttgtgggt tctgcaatat aacccagcac ctgaccttcc
     2341 ttcacatatt catttgctgt taccttcagt tcttttaact gaccgcagac agccgtatac
     2401 ttgttgccca tgtttaaaac aacgtagtta ccaatttctt cattggaacc gatttcctgc
     2461 acttttgcat tggcgggggc gactaccgga gtgctcacat ctccctgaat taccacgcct
     2521 ggattgcatt tatactgatc cagggttggg aagtagatgg tcttatccat actgtagtcc
     2581 aaaataacat ttccgcgtac cggccaggtc attctggtgg agtcgttaaa attaagtacc
     2641 agtgacttgg cggcatttgt ctgctgcggc ttttgctgcc tgtgtttctt tttcggctgt
     2701 ggctttggct gaatcaacag ccttcatttc ctcttccgca ttttttgttg cattggaatc
     2761 tcctgcaaca gctacctgtg tatcctgggg gactgattct tcaacaatca tattgctctg
     2821 atccggtact ttcagataag ggctttctcc tccaccattt cctctttgaa cggtaatgac
     2881 tcctgctgca gctactatag tcagcaggcc tagaaccaac aggacaagga atactttatc
     2941 cttgaacaat tgacttattt tctccttcac gtttatcacc tcgataatat tcttaccaag
     3001 agtgaaagag ttattcagat ttcaaaacaa tatttttata aaaataagca agaatttttt
     3061 ctgccgtcca tccttcctca cttttacatt ttgcaccgta ctggctaaag ccatatccat
     3121 gtccgatccc ccggcagacc gctctcactc cgccctcata atcttcaaat tcaaaggaag
     3181 gcgatggcag ggaaagtgca tactgaacct gatctccggt gagggtatgg gtaccgatct
     3241 ggatctgtac cacgtaaccg gaatcatccc gctgaacaat ctgaatgctt tggggaatct
     3301 gatctgcctt tacagaacca tctcctgaga tcttgttgat tttatccgca aagtcttctt
     3361 tgctgaactg aagaattgtt aaaaaaccct ctgcctccac atcctttttg cattccaccg
     3421 gctgtaagta tgggtggttt gaatcaccag ctcttgtcat tccgttgctg gcacgatgaa
     3481 acaatggttc aattaattta tcgttacagg tgatcaccgt acctgtggta gagcgtaccg
     3541 catcttcaat tgcctgatag ctggtgacaa aggcctcgct tccccacatt ttctccatct
     3601 gcttctgttc taaataatcc atatggagat ccgattcctt tacttctttc tgatcattca
     3661 ttttaccata aatataggtc ctggcgatga ttgcctgggc ccggagtgct tctttcccgt
     3721 aatctgccgg catctgcttt gctaccactc ctggaagata ttcctccaca tccatggatg
     3781 tttcaccgcc ttttctgtct aagatcacac gttttccact gagtgtaatg ggcatttctt
     3841 ttttctcctc aattttcccg gtccacgcca gagttataat atatggaaac aaaaatgcca
     3901 gtatacatgc cattaaaatc tttttaccca tcttcccgcc gcctttcttt cagtctgaac
     3961 ccttgttcaa tatatgcgaa aaagcgcccc ggaatcactt ccggaacgct ttttcagaaa
     4021 ccttcatatg gatcagagtt ctacatttac cttttccaga tgccagatgt ctctgacata
     4081 ttcaagaatg gtacggtctg atgtaaattt accgctgctt gctacattta aaatggcagc
     4141 ttttgcccac caggattcat ctctgtatgc ctgatcgatc cgcttttgtg cctctccata
     4201 agaaggaaga tcctttaaaa taaagtacgt atcggcacgg tcactgctca tggtgttaag
     4261 cagggaatta taaatatcac ggaacagctc cggatcctgt ggggaataat agccattaat
     4321 cagctgcatc aaaactctgc ggatctcata gttattgttg aaaatctcca tgggatcgta
     4381 tccgccttcc cgctcataac ggatcacctc atcggatgta agaccgaaaa taaatgcatt
     4441 ctccgctccc acttcctcca cgatctccac attggcacca tccatggttc caagtgtcag
     4501 ggcaccgttt aacatgaatt tcatattgcc ggttccggag gcttctttgc tggcagttga
     4561 tatctgttca ctgacatctg ctgcagcaaa tatgatttct gcattggaaa ccttgtagtc
     4621 ttcaataaat acaactttta ttttgccgcc gatgcttctg tcattgttaa ttacctctgc
     4681 cacggaatta atcagcttga tggtaagttt cgccctgcga taaccggcag cggctttggc
     4741 tccgaaaata aaggtccgtg gaaccatatc cagattagga ttatccttca gctgattata
     4801 cagatacatc acatgaagaa tgttcatcag ctgtcgttta tactcgtgaa gacgctttac
     4861 ctgcacatca aatatggatc tgggatctac ctctatgccg ttatgttcct taatatatct
     4921 tgccagccgg accttgttct gatatttgat atccataaat tcctgctgac atttgggatc
     4981 atctgcgtaa acagccaggc ggtgaatatg aggcagattg gtgatccatt catttccgat
     5041 tttctttgtt acccactcag acaaaagagg atttccatgt aaaagaaaac gtctctgggt
     5101 gataccattg gttttattat taaatttctc cggcatcatc tcatagaaat ctttcagctc
     5161 ctgattcttt aagatctccg tatgaagtct ggctacaccg ttaacggaaa agcctccgac
     5221 aatggcaaga ttggccattc gtacctgacc gtcataaaca atggccattt tacggacctt
     5281 atcctggttt cccggataca tctggctgat cttctgctga aaacggcggt tgatctcttc
     5341 cacaatctga taaattctgg gaaggagtct ggagaacagc tcgatgggcc atttttccag
     5401 agcctctgac ataatggtgt ggttggtata agcacaggtc ctggtagtaa tctcccatgc
     5461 ctcatcccag ctcaatccgt agtcatcaag gagaatccgc atcagctccg gtacagccac
     5521 cgtcgggtgg gtatcgttta actgaaatac gttcttctca tagaatttat ggatgtcatc
     5581 atggttttcc atatacttta tgactgctct ctgcacgctg gcagagataa agaagtactg
     5641 ctgctttaag cgaagctcct tgcctgcata gtggttgtca ttgggataga ggacttcaac
     5701 aatggtttta gccaggttct cctgctccac tgccttttgg tagtccccct tgtcaaagga
     5761 atccaggctg aaggtgttaa tggcctgggc atcccagata cgcagagtat ttactacgtt
     5821 attgccatat cctatgatgg gcatgtcgta aggaaccgcc aggacggact gatagccctc
     5881 ctggacaaac cgctccttac cattctcccg taccaccttt acatatcccc cgaatttaac
     5941 ttccgtggca tattctgccc ttcggatctc aaagggatat ccgttcttca gccagtcatc
     6001 cgggatttca acctggtaac cgttctcgat tttctgtttg aacatgccgt aacgatatcg
     6061 gattccacag ccgtaagccg gatatcccag tgttgccagt gaatcaagaa agcatgccgc
     6121 gaggcggcca agccctccgt ttccaagagc cggatccggc tcctggtctt caattgcatt
     6181 taagtcaaac ccaagttcct ctaacacttc ctttacatcc ggctgtgcca tgaaacttat
     6241 gatgctgttg ccaagagcac gacccattaa aaactccatg gacaggtaat acagggtttt
     6301 ttacatcctg cttttcgtac tctttgtggg ttgcgatcca ttcatctatg atcacatctt
     6361 ttactgcata ggcgactgcc tggtacatct gctctttggt tgcctcatct actgttttac
     6421 gaaacacgtt cttcatatta aacaagacac tttttttaaa ggtttcctta tcaaatccca
     6481 tgctcattcc acaagacctc ctgactgttt actggatctt ctccacgcct aaggttacat
     6541 tctctccgtt aatattccat tccttcacat aaccggcagc ttcatttaat atgatatcgt
     6601 ctgcaaggac ctcgttctta acgtcgtttt catgagcctt taaaatactc tcgatttttt
     6661 cattgtcttt gctgtatacc cggatgtggt ctaccacctc aaaaccagct tctttacgca
     6721 tcgtctgaat cttgctgatc agctcgcgta caaatccttc tgctaaaagc tcaggggtta
     6781 aattggtatc cagaacaacc gtaatgccgt tgtctccctc tgatacatat ccttccatct
     6841 gggcggtatc aatgagtaaa tcttcttttg taagcactac ttccgttccg tcaaaattaa
     6901 aggtaagagc acctttttca ttgagggtat ccatagcctc attgccgttt atctctgtaa
     6961 gcgcattttt aatgctgcct aactgcttgc cgtactttgg accaacggtc tttaactgtg
     7021 gtttaaagct gtaagaagtg aagtccctta catcctcttt aaatacaact gttttcacat
     7081 tcagctcttc ttcaataatc tcctgataga agacaggaag tgtatgagga gcctttacaa
     7141 acatctgacc gattggctgg cggtttttga tatttgcagt atttctggct gcacggccca
     7201 ggactacaat ttttaaaacc tcatccatat tatcttccag ctgtttgtcg atgaaggttt
     7261 cattggctgc cggataatcg cataagtgaa tgctttccgg agcggaactg tccacactgc
     7321 aaaccagatt ctgataaatc tgttctgtca taaatggaat aagaggagca gcaatcttgc
     7381 tcacgtcaac cagggcggta taaagggtca tataggcatt gatcttatcc tgctccattc
     7441 ccttagccca gaaacgctct ctgcttctgc gtacatacca gttactcata tcatccacaa
     7501 attcctgcaa tgctctggct gattccggaa tctggtaatt gccaaggtaa gaatccacct
     7561 gtttgattaa ggtatttaac ttggataaca gccatttatc cataactggc agcttctcat
     7621 aatccagttt gtatctggta gggtcaaatt catcaatatt cccgtagagc acaaagaatg
     7681 cataggtgtt ccacaaagtt cccatgaatt tacgctgtcc ttccataact gcttttccat
     7741 ggaaccggtt tggaagccat ggtgcactgt ttacatagaa ataccagcgg attgcatcag
     7801 ctccataggt ctccagtgct tcaaacggat ctaccgcatt gcccttggat ttactcatct
     7861 tctgaccgtt ttcatcctga acatggccaa gtacgattac attcttatat ggtgcctgat
     7921 tgaaaatcag ggttgaaatg gcaagaaggg agtaaaacca tcctcttgtc tgatccactg
     7981 cttctgaaat gaagtctgca gggaactgtt tctcaaataa atccttattc tcaaatggat
     8041 agtgatgctg tgcaaatggc atggaaccgg aatcgaacca gcagtcaatt acctctggta
     8101 cacgcttcat ctgtttgccg cactgaggac aggtaatggt aaccccatcg atataaggac
     8161 ggtgaagctc gatctcatcc gggcagttgt cagacatact ctttaattcc tcgatgctgc
     8221 cgatggaatg ctgatgtccg cactcacatt cccatacatt taaaggagtt ccccaataac
     8281 ggtttctgct cacaccccag tcctgaacat tctcaagcca gtcaccgaaa cggccttttc
     8341 caatgctctc aggaatccag ttaatagtat tgttattgcg gattaaatcg tcttttactg
     8401 ctgtcatctt aataaaccag gattctcttg catagtagat gagaggagtg tcacatctcc
     8461 agcagtgtgg gtagctatgc tcaaataccg gagcggcaaa caaaagaccc tctctcttct
     8521 aagtctgtta aaataagggg atccgctttc ttacagaacg tgccagccca ttttgtttct
     8581 tctgtcatct ctcctgcacc atttaccagc tgcacgaaag gaaggtcgta ttttcttcca
     8641 accttggaat cgtcctcacc gaaagcaggt gcaatatgaa ccacaccggt accatctgtc
     8701 aaagtaacgt aattgtcaca ggtaacgtaa aacgcctttt tgtttggttt tacaaaatca
     8761 aagagaggct catattcctt aaattcaaga tctttaccta caaatctctc taatacctta
     8821 aattcttctc ccaatacctt ctcacagagt gcctctgcca tataataggt atattcgccg
     8881 ctttgtacct taacataggt ctcagacgga ttcacacaaa gtccaacgtt ggaaggcagg
     8941 gtccatggag ttgtggtcca tgcaaggata aatgcatcct cgttttttca ctttaaatct
     9001 aacaattgca gagcgctctt ttacatcttt atagccctgg gcaacctcat ggctggataa
     9061 aggagttccg cagcggggac aatagggaac aattttaaag cccttgtata aaagcccttt
     9121 ctcccagatc tgttttaaag cccaccattc ggactcaata aaattattat cataggtaac
     9181 atagggatcg tccatatctg cccagaaacc tacagtcttt gaaaaatctt cccacattcc
     9241 tttgtactgc catacacttt ccttgcagta tttgataaat ggttccagac cgtattcctc
     9301 aatctgttcc ttgccgtcaa gtcccagctt cttctcaacc tcaagctcta ccggcagtcc
     9361 atgagtatcc cagccagctt tacggggaac atcatatcct ttcatggtcc ggtatctggg
     9421 gatcatatcc ttaataaccc gggttaaaac gtgaccgatg tggggttttc cattggcagt
     9481 aggaggtccg tcatagaagg tataagtttc tgcttccttc cggttatcca tactcttttt
     9541 aaacacgtca tgttcttccc aaaacttttc aatttccttt tctctctcca caaagtttaa
     9601 atctgtaggg acttttctgt acatctttat aacctccatt aaaattttta atcaaaaaaa
     9661 cctccatcct gtaatcagga cgaaggctga attcattctt cgttatacca cctaataccg
     9721 catactgaca tatgctattc tttaacgcag aaactacgtc ctgacctact aaatgtattc
     9781 agccggcaac tccggagtga tgttcgaatc cataatactc tcaccaggtt tccaccctcc
     9841 ccggctctct gtagatttca cacggttcta ctgtctccat caaagtcttt ctatgtctac
     9901 taattatagg gagcgaaaaa agaaatgtca aggttatttt tgtattcagc tgctgcagtt
     9961 taacaaaaag gagggtatcc cctccctttt gcatcctgca ttatgaaagt accagtaaaa
    10021 atgccgctgc agtgatacca catatgaaaa gatttcccgc atagcagtgg tgaaacgcag
    10081 tcagtaccag gcagcctgca ttggccagga tcagaatcaa tcttgctttt cttctgtagt
    10141 gtaccacttc tttctcatcc agtcttttat tctcattatc tgccggactc ttaaaaaaca
    10201 gaaccgcatc cgacaaggcc agtgcacccc accagagaac ctgattgatt tgagcgtttg
    10261 acaggaaaag aattatggct atatagcaac aggaaaatat gtaacacccc attttagtct
    10321 tcgcatgata accgcctgca tttcttctaa gcggtataag gacacagcca ataacaatta
    10381 attgcggcac catctgaaaa caggcagcaa tgcataaata ggaaatacag tggatcagtt
    10441 ttaaaaccag acactcgatt ccaaactgat ataaatctgc ctctttttcg ttgataattt
    10501 cattggatac cagtttttct acaaatgttt cttcccatct tatcatctgg attttcatcc
    10561 tagaattttc ttaatttttt tgcgttatcc ggtaatttag gctggtagcc gataaaaaag
    10621 caggaactgt taacctctgt ttttgctgaa tttaaaatgg cttttgatgc aattttaaat
    10681 gatagttttt tcaattggga tttcatagga atgccttctt tctattttta atttttatta
    10741 ctatatgact tgttttcccg tattatacgg ctaactgaac cgttacagga ttttatacat
    10801 gaatcacctt taaatccggt gctacgttaa ggtgggcttc tgttgcattc tggacatctt
    10861 ccacggtcag acccggggca atttccttta atacaagccc cttgtcagga accacatcaa
    10921 ttacagccat atcagtgaca atggtcttta ccacattcac tgctgtcagc ggaagcctgc
    10981 attttttcag tattttcgga gctccctctt tgcttacatg ctccatggct aaaatcattt
    11041 ttttcgctcc aaccaccagg tccatagcgc ctcccatacc aggtactttt ttgcctggaa
    11101 tcatccaatt cgccacgtta cctttttcat caacctccag cgcacccaga actgtaacat
    11161 ctacgtggcc tccgcggata atgcaaaatg aaaacgaact gtcaaaatag ctggcgccct
    11221 ttacagttgt tacgtagttt cctcctgcgt caatgcagtc cagagtttta tcctcctgat
    11281 ctgcattggg tcccacaccc aatattccat tttcggcctg cagcataata tgtacatcgt
    11341 ctggtatgta gtttgcaacc attgtaggca ttcccacacc aaggtttaca acatctccgt
    11401 cttttagttc ttttgctatt ctccttacaa tcgattctct tttattcata atttactggc
    11461 ccacctttac aatattatct ataaaaattc cgggaacagt tacatcattg ggatcaattt
    11521 cccccgcttt tacgtattca tccacctctg cgaccacata atccgcggcg gtagccatta
    11581 ctacattaaa gttgcgggag gagccgttga taatcagatt tccagtctca tctgccttct
    11641 gggcttttat aaatgccaca tttgccttga gcggcatttc aagcagataa tttttctctc
    11701 cgatctctat tttcctcttt ccttcttcta caactgtacc cagcccgact ggtgtcagaa
    11761 ctcctcccag accagctccg cctgcacgta ttttttcagc aagggttccc tggggaaaca
    11821 actgaacttt ggcttcccca gtcataagaa gtcttccggt ttccggattt gacccaatgt
    11881 aggaagcaaa gatccgtttc actcttccgc tttttatcag ttgatatatg gaaagctccg
    11941 ttgttccggt atcattggat atgatcgcca ggtcactggc tgtactggta tttagaagtg
    12001 catttaccag attttcagga tgacctcctg ccagaaatcc cccaatcata atagcatctc
    12061 catctttgac ttttttcacc gcttctcccg ctgatattaa tttgttcata tagaatggcc
    12121 tcctttttta ttcacttagg taatcatgga ttacgcgtcc atatggtaag gttaaatctg
    12181 agatgatatg cgttccgccc atgcaggctt ttacaggcag atctgccaca ggcgcattca
    12241 catggggaat taaatgaagt cttgcaggcc cggaccatgc ctcctttacc tgaatatctt
    12301 ccagattgta agccaccagc tgggcaatct ttggacttcc gtctgcatcc ggaatcagct
    12361 ttaaattaac ctgcattttc cccaggttct tagcaactgc ttttttatcg agaatattat
    12421 atttaaaggg catggttccc attgctacaa gaatattatt gtaaattaag gtaccggtta
    12481 atgtctcggt gtcctcaact ctaaggtggg gctggcccca cttctttggg aaatccccag
    12541 atttcacgtc ctgcaagaat ggcaggaccg ttatttaaat acatttgtgc tgtaaaatta
    12601 caggcttctc catggtatgt acatggaatt acaatcccgc tttcttcgta ggttccaaga
    12661 cctgaggaat cgggcatctt gatccattca tacaaaacaa gattgcttcc atccggttcc
    12721 agcggttccg gcaccgccgc tttaatcaga tccggatctg attcatagct gactgtaaaa
    12781 tattccctgt ttataaaacg gcacggaggg cgcccataac tggcgcttgc tgcaggcatg
    12841 gactgcaggg agataatcgt ttctgatttc ataaacatgt tcctttctat gtaaaagttg
    12901 tttataagtt agcattttca accagcatgg caattcccat acccccgccg atgcacaatg
    12961 atgccacacc gtaacgagcg gatctgcgca tcatttcatg taccagtgta accagtatcc
    13021 gggcaccgct tgctcctaga gggtgtccga ttgcaactgc cccgccgtta acattcacct
    13081 ttgacatgtc aacaccaagt tcctttgtta ctgccactga ctgtgccgca aatgcctcat
    13141 ttaactccag taaatcgata tcatgaatgg ttaatccctg tttttcaaaa gtgtagaaac
    13201 tgcctttacc ggccctatgc ccatatatgc aggatcaacg cccaccagcg aatagccttt
    13261 gatataagcc attggtttta aatcatactc tctgcattta tcttcggatg ccacaagcat
    13321 ggcagctgca ccatcactga ttggggatgc atttccggct gtaactgttc cgtccgtttt
    13381 aaatacagtt cttaacccgg aaagcttttc aagtgtggta tctggtttga tatgttcatc
    13441 ttctgagaag cagatttctt ctttcttctt tttaacggtt attggaacaa tctggctatc
    13501 aaagatctta gcattttttg ctattgctgc cttcatatgg ctgttatagg aaaactcatc
    13561 ctgctctttt ctggttacat tgtatttttc tgctacattt tctgccgtaa ttcccatatg
    13621 ataatgattt atggcacagg taagagcatc ggcgaccaga ctgtctacca gttctccatt
    13681 acccattcga taaccgtttc ttgctttttt caaaagataa ggtgcatttg acatgctttc
    13741 cattccacca gctaaaacaa tgctgttttg ttccgccaga attgaatcat aagccagcgc
    13801 aaccgtatgc agcccggagc cgcagaccgt atttatggtg gaggcaggaa tctcaaccgg
    13861 aataccagca tttacagcag cctgtctggc agggctttga ccaattcccg cctgaagcac
    13921 atttcccaga tatactgcat caatcagtga actctctaat ttactttgtt ccaggcagga
    13981 ctgaatgacc gctgttccca gttctgatgc atttaaggtt gaaagagtac cgttaaagga
    14041 accaacagca gttcttacgg ggcttattaa agctattttt ttcattttat ttgctccttt
    14101 ctattctcat ttcttgttta cagccacatt ctcctacttc ttattgtggt tttcaactaa
    14161 tgtcgtacac cttacagttt ttatgtataa tctacaactt tttgaatgtt tcattcgtga
    14221 aacatcaggt gtaaataatg atcagaaaac aaaaagaaag cacaccggac tttttttata
    14281 ccagtccgtg tgctttctat tcaacaaata atatatctta attaaatatt attttacagg
    14341 agaagatcct gttttcttct tccaaaacca tacttccatt atactttttt attatatttt
    14401 ccacatttaa aagaccaatt ccatgatttt tccctttttt tgaaactggc ttgccacgat
    14461 gccatctttc tatgggggct gtgctgttgg taatatttat aactgtaaaa tcattatatt
    14521 tatcaagctt aactttgata aatctgtcct gtggcacctg gtcacatgca tcgatggcgt
    14581 tgtccagcag atttccaaat attgtcgtta cttcaattgg ctccataaag gaaagatcaa
    14641 caacgcctat ttccagattg aactttattt tatgaaagat ggcaatacgc ttcttatcat
    14701 ttaatattac atttaaaata ggattattgg tatagtcctc taaagacaga ggaatcagta
    14761 atcttcctat ttctttggca taattaagtg ctgctttatg ttcatttgtc cgatacatct
    14821 cctcgatcag attcagacgc ttatatacat catggagtat tataatggac tcattatact
    14881 tttctttctg atcttcataa aattcatact gcagcaaaga ctgttgctcc aacaatttta
    14941 attttacttt tagctggttg ttttcttcta caaatcttgc cagatataaa aaataaagat
    15001 ctgcaaataa gatgcacccc atattaatga gtatcagaat atattctgat atatttgtaa
    15061 ttttggggat taccacgatg attacagcta tattggtcat actgaacaat ataattataa
    15121 cttgtatcag gcattgggta attgtatact taacagcaaa atcttcccgc cagagtctgg
    15181 tgataatcag ataatacaca atgaggacga ctaacttgga aaaagttagt tccatacttt
    15241 taatcataac aggctgaatg ccgtgaatct gaaatttcag aagtatgtat tccagcatta
    15301 ccaccccagc cgtttcgcag acagttaata ttaaaatcaa aaccgggact tcaaagatcc
    15361 ggtatattaa ttttttattg tcattgccat agaaaatatt atttattaaa ctaaacagca
    15421 caatccagat tattatatta aggactgggt gccccaattc atttatcatt acaactataa
    15481 aaaatgcggc tgtctttaat aatatgttaa aaaaaatcgg ttatttattt tccgttcatt
    15541 tctttcttct ataaattgaa atagaataat gatattaatc atacatgaaa tgaaggttcg
    15601 aatgtaagaa tcatataatt ttccattttt ttctcctata cattactctg tatgaaatta
    15661 tttagttttt tcctgaaatc cgctgatttt ttctgggaaa caggaagttt atcattatta
    15721 tttaacagaa cctcgtaacc ttttatattt ttaatatgtc ttagatttac cagaatgcat
    15781 tgatggcagg cttcaaaatt gtgcttttgc attctttccg ttaaattttt cattttatct
    15841 ataaaaaaat attcttcatt ttcaagtttt attttaattt ttctatttat atattcaaag
    15901 taaaaaatgt ctttgatttc aaaacttttt attctgcttt ctaattcacc agcctctgtg
    15961 acttctaaaa tttcaaaatt aacaatctct aattttccag attcttcctg aatgtattgt
    16021 ataatctcct gaagctgacg ttccacattg atctttgtaa cgggtttctc caaaaaggca
    16081 aacgcatgaa cataattgac agcctgtctg caaaattgtt tataactagt ggtatagatg
    16141 actttaacat tccggttcat ttcccatatt gttttaccaa tctgaattcc attcattccg
    16201 gttaaggcaa tatcaagaaa tatcagatca aacttaccat gaaattcaag cagctcttca
    16261 cctgacttaa aagttataat ctgaaatgga attgatacgg atttcatata accttttaca
    16321 aattttgcta ttctgactga atcactttta ttatcatcac aaatggcaat aagaagcatt
    16381 aataccctcc tcttctgata ttttatattg aagtaataaa acgcttacat tctatatcct
    16441 ttttatgtac atttcaaccc aaccgccaaa attgtacatt ttgagtgcta atatttacat
    16501 atttgcctct aaattgtgat gacgtctatg aaacaaaatc agagcgggta ccgaattctt
    16561 tcctcaattc gatacccgct ctacacgttt tcatccatca gttaattcat gcctctcttt
    16621 agatttttga taatgctttt ctggatgatt ctctatatga acttgttttc gcttccctta
    16681 ctgatccctt atgcaatctc tattattctg cttcttattt tcattcttct atcttgtcag
    16741 ggaaccattg ggtttgggtc gctggaaata accagcgaaa ttactttaat atagttctga
    16801 ccaatcatag gcacgtcaat gaattgccgg acccttctgc catcttccat gtcagcttaa
    16861 ctgctggaat tcactggctt ttataaagtg gggaaacgtc ttatcttttg cctgagcact
    16921 ttgtcagtca acaggcgtta aactgttttt ggtggaatcg acctcctttg gctctgtctt
    16981 atgaattatt ttttaaaaaa gggtgtaact gttaatcttt tgtagatgat attattataa
    17041 tgcatagcgc tttattttgc aagttatttt ataaattttt aatcttttat ttttctattt
    17101 actaattgtg tatgtttatt ctgataattt attctattta gacactttaa ttgtttattt
    17161 gcactccata gagattgtta catttttatc aatgtaattg tttttttgta aacatatggt
    17221 ataatgtgcg aaaaggattt gtaaaaagga gaaaaattat gagccagcgc aatttaactt
    17281 tattaacaga tctctatgag ctaaccatga tgcagggtta tttcaaagag aaagatgcca
    17341 acgagaccgt tatattcgat gccttttacc gcagcaaccc aggcggaaac gggtacgcga
    17401 tctgcgctgg tctggaacag gtgattgaat atattaaaga actggaattt aacagtgagg
    17461 atgtggacta tttaagatcc accggactgt ttgaggaaga ctttttagag tacctgcttc
    17521 attttagatt ttccggagat atttacgcca taccggaagg aaccgtcata tttcccagag
    17581 aacctctggt aaaagttatc gcccccatta tggaagccca gctggtggag acagcgctgc
    17641 tcaccattat taatcaccaa agcctgattg ccacaaagac cgcccgtgtg gtatttgctg
    17701 cggaaggtga cggagtaatg gaatttggtc tgcgccgtgc ccagggacct gatgcgggta
    17761 tctacggtgc ccgcgctgcc atgatcggag gctgtgtggg cacctccaat gtcctggcag
    17821 gaaagatgtt taacgtcccg gttaagggga ctcatgcaca cagctggatc atgagctttc
    17881 ctgacgaact atctgcattt cgcagttacg caaagcttta tccaaaagcc tgcattctgc
    17941 tggtagacac atacgatacg ctgaaatccg gtgttcccaa tgcaattaaa gttttcaagg
    18001 agatgaaaga agcaaatatt ccactgacca gttatggaat ccggttagac agcggagacc
    18061 ttgcctatct ttccaaaaag gccaagaaaa tgctaacgga agcaggattt cctgatgcca
    18121 tcatttctgc ctccaatgac cttgatgaga ctctgatcaa cagcttaaag atccagggtg
    18181 caaccataaa ctcctgggga gtgggtacta atttaatcac ctccaaggat tgtccttcat
    18241 ttggaggagt gtataaactg gcagctgttc tggaccggaa gactggagaa tttaatccca
    18301 agattaagct ttcagaaaat gcagaaaaga tcaccaatcc tggcaacaaa ggaatcaaac
    18361 gaatttacag cagggaaacc ggaaaaatta ttgctgattt aatatgtctg gaaggcgaag
    18421 tatatgatga aaatcattca ctccttctct tcgatccgat tgagacatgg aaaaaaacac
    18481 acctggcacc aaatacttat accatgaggg atttactgat tcctgtattc cagaagggca
    18541 aatgcgtcta tgaatctcct gccgtaatgg acatccaggc ttattgtaaa aaagagctgg
    18601 ataccctctg ggatgagtca agacgtcttg ttaatcctca tgaggttcac gttgacctgt
    18661 ctaagccgct ttggaacatg aaaaaccagc tgttggacaa ctatcatttt caggaatcct
    18721 gattatatta aggcataaaa aacggcagtc cttttacagg ctgccgtttt cctgttattg
    18781 ttcacattct ggtcacaata ttgttacatg ccggacaaac tctcttcata ttttactcat
    18841 atacttaaat catcagaaaa agatgttgcc aacatacttt gagataagaa tttttttcat
    18901 aattgccggt attcctcgta ggggataccg gctcctccct ttttaggggg cttttttaca
    18961 tctgcttctt tacgatagaa taatcatccc catatttaaa gtgagggcag ctttgatggg
    19021 attcacttaa aaaacgcatc atctcatcct catctaaatt aattccacac tcataatatt
    19081 catagtcttc gttatattga taatacatac aatattcaca actgcttttc tttgccatta
    19141 ttgatctcct ttatctgtca tttccccgtt tccatatcaa tttcggaatc ctttttaaca
    19201 gtttcattac cgtttcatcc aataaccagg ctattaccag agaaatgaaa atgtcagtgc
    19261 cgtcaggcag ataattttag gaatgtaaaa atgtctgatc catgttttct ctatgattgc
    19321 agcggagaac tgtcctaaca ccagataatg aattaaatac ataccatagc tgtgtctgct
    19381 gatcaatccg catgcgtttg taaatacagg gaatgatacc gttccatatc gttcaaaaaa
    19441 taaaaagaca gctgatgcca tggctatcat agtgggtgcc agatcatgaa tacctggtgt
    19501 aaatccaggt gtataaaact tctgaaccat ggtcagcgca aatcctgcaa gtcctgctgc
    19561 caggaaaggg aaatacctgg tgttttgata taaacgcttt aatatataac caagcacaaa
    19621 gtaaatcagc cagcctttaa atatcatata ttgaaaaaac tctcctggta aaaaaccaag
    19681 cggctgcaga taaacaaaca aagtctgaat cataagaatc atggccagaa aaatcctcag
    19741 ctcatgatct gtcatagcct ttagcattcg tgataaaaag ggcgcacaca aatacaaccc
    19801 cagaatcacg tacataaacc acatgtgtgg agccatgggc gtagcttctg tcagccaccc
    19861 aaaaagccat gctttgatat gcgcaaagaa cccaagcttc tcctgcccca ggtatacatt
    19921 gtaatagtaa taataaattc cggcatagaa accaaatgga atcacaatgg tgcaaaaccg
    19981 ttttttataa aatgccagga tgttctctgt accatgatca agaataaaca aaccactgag
    20041 cataaaatag attcctacaa aagacaaaag cacaggcata agcattgcac taaaagctgc
    20101 tgcgtatcat tggctgcctg ggcaggaatg gaatgcacca taataattgc aataactgca
    20161 attacccgca gataatcata ctgaatttcc cgatttttcc gttccatacg ttatcttaag
    20221 cctccttttt tctattgcaa agtataacat aaaaaatctg cttttgacaa tttttttatt
    20281 tttttgaaaa aacagtagac aaaccagaat acctgtgata ttataatgga gctgtcagag
    20341 atgacaggga aacggcctat tggtcaagcg gtcaagacgc cgccctctca cggcggaaac
    20401 ccgggttcga ttcccggata ggtcataggt aaaaagcgag ttttcaatta gaagactcgt
    20461 ttttttttgt tgagatatta atatattttg cataaaaaaa aacatgggca ggacattttt
    20521 aacgtctcgt acccatgtct tttcta
//
