LOCUS       NZ_CP070896            20650 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Lacrimispora xylanisolvens strain ASCUSBR21 chromosome, complete
            genome.
ACCESSION   NZ_CP070896
VERSION     NZ_CP070896.1
KEYWORDS    RefSeq.
SOURCE      Lacrimispora xylanisolvens
  ORGANISM  Lacrimispora xylanisolvens
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Lacrimispora.
REFERENCE   1  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Hungatella xylanolytica ASCUSBR21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) R&D, Ascus Biosciences, 6450 Lusk Blvd E209,
            San Diego, CA 92121, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAY-2019
            Assembly Method                   :: Canu v. 2.1.1
            Assembly Name :: Hungatella xylanolytica ASCUSBR21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017084465.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,443
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 686
            CDSs (without protein)            :: 686
            Pseudo Genes (ambiguous residues) :: 0 of 686
            Pseudo Genes (frameshifted)       :: 599 of 686
            Pseudo Genes (incomplete)         :: 95 of 686
            Pseudo Genes (internal stop)      :: 24 of 686
            Pseudo Genes (multiple problems)  :: 30 of 686
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:11:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 380996
            Orig. end                         :: 401646
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20650
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="2"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20650
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20650
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10650](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10650)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            complement(1879..2899)
                     /gene="pheS"
                     /locus_tag="JWH23_RS02110"
                     /pseudo=""
     CDS             complement(1879..2899)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pheS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442524.1"
                     /locus_tag="JWH23_RS02110"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKDQLEKIKQEALKQIEASDALEKLNDIKVAYLGKKGELTSVLKS
                     MKDVTPEDRPKIGQMVNEAREAIEGKLDAAMTALMKKAREEQLKKEVIDVTLPAKRNRV
                     GHTHPNTIALEEVERIFVGMGYEVIEGPEVEYDHYNFEKLNIPKNHPARDEQDTFLYQ"
     misc_binding    complement(2955..3198)
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
     misc_feature    2995..3011
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.57"
                     /tool="antismash"
     gene            complement(3273..4334)
                     /locus_tag="JWH23_RS02115"
     CDS             complement(3273..4334)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="JWH23_RS02115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="WP_277407774.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 4.6e-12,
                     bitscore: 44.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVKNNYIIIPAYEPDWRLIHLLKELNERLNAVFVVVNDGSSREK
                     DEIFCEAENFAVVLHHDKNYGKGRAVKTALSFIKNQEYAGRIVIADADGQHTPEDIALL
                     LGVKEGERNVLITGSRSFQGEIPFRSRLGNQFTRATFWLLSGKWLKDTQTGLRAFHSSL
                     IPMFLEISGDRYEYETNMLMTCVKENIRIVEIPIETIYLEGNKSSHFRAIKDSARIYMT
                     MLKFVSSSFLSFCMDYLLFGIFLSLTSGLGDITADAVSNIMARIVSASFNFFVNRNYVF
                     RYKGSLLQSALGYAALALSILAVNTAMLLWLVRDWGMGGLEAKIVVEVSIFFVNFLVQK
                     FFIFKKRPVHTAL"
     gene            complement(4420..6492)
                     /locus_tag="JWH23_RS02120"
     CDS             complement(4420..6492)
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /locus_tag="JWH23_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_277407775.1"
                     /transl_table=11
                     /translation="MKRRVGIRSIGIKMLLGILPVVVLALALLAKLSESTSRELIEQQS
                     NSTMESELKANVNGIDNYLNAVETTAMNISHIVGASYQSTDLDTYGKAIADIISGNDLI
                     LGSGIWFEPNVYDPQQKYVGPYWYKAGNKVTLTNEYSNESYDYFNQAYYKSVSGGTKDA
                     IITDPYFDPTLNMMMASCTAPIYDSSNRFIGAVTVDMQLSDIDELIKSIKVGKNGTAVL
                     ISGEGVYLCGADAEKVSKGVNMTQDENASLSAAAAQMVANDKGKTVYTEGGETYNLYYD
                     TVPGVGWIIMIKMPQSELAEPIVALNEKLAKISAAALVLCIIAVLFQVGSITSGLKKVR
                     KFAGSLADGDFTVQPLKSRRKDELGQMSSSLNNMFQSNKGVIEQISDQAGDITDASIHL
                     NKVSTGLLVQFSDVKTYMSDVDKAMMSSSAATEELNASLAEVNSSVGVLASETEKSSKM
                     AGEIMERAKDIEKKSREAYDNAIVISQEREQDLQMAVQHAKVVERIGDMARLISNTASQ
                     INLLSLNASIEAVRAGEQGRGFAVVAREIGKLAKDTSEIVKEIEGTITDVQNAFSMMLD
                     GSQSMLLFLRDTVTPDYDNFVNVGQQYGRDAVTVEEISNEVEEMATNISQVMQEVRDAI
                     QNVSESVQTTAGNSSMVLSTIEKAYEVVQDMSKMSEKQERIAEELKTVVSGFKLK"
     misc_feature    complement(6544..6557)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.28"
                     /tool="antismash"
     gene            6840..7151
                     /locus_tag="JWH23_RS02125"
     CDS             6840..7151
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314911.1"
                     /locus_tag="JWH23_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_104437610.1"
                     /transl_table=11
                     /translation="MDQDYINKRITELRLKKGVAEHKMSLELGHSRSYVQSISSGRSLP
                     SMAEFLAICEYLSVTPKDFFDNDNPNPPLVRDTIDKLKSLPESDLLLILTMINRLQSK"
     gene            complement(7244..7408)
                     /locus_tag="JWH23_RS02130"
     CDS             complement(7244..7408)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407776.1"
                     /transl_table=11
                     /translation="MTKDQTFQDLYGYFFGMINRGDLDELYVRALEAIPSIVSLDEIND
                     VKNHQVSAV"
     gene            complement(7405..8481)
                     /locus_tag="JWH23_RS02135"
     CDS             complement(7405..8481)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS02135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407777.1"
                     /transl_table=11
                     /translation="MEQLRIYLDSGEMFYPTQCFIDKRVNQHFTAVVTGVVNSDKDNLI
                     LRESKEVLLTITFVNEEKKEIPIFKGRIERRYEIEGDGSVYWNLFAVSLTMDLDREKHI
                     RTFQGEGQNYLQIIGSVLDGYENTTVVLSKEMRETSDGLAVQYMETDWQFLIRLAAKKN
                     QILVADSVHNNICFHFGRIKRKGRKCFNSDQYRIKCYDDYEKGETVEYLIKSRKCLHLC
                     DCVMIEDSAYYIYSMSMNFSGGKVEFDYVLRTRSGFLVPAKEHLSLAGLMIMGNVKNTG
                     ENAVQIKLLSEKDETYGKPICFPFSSVYTSPEGMGLYVTPRPGDPIRLYLPDGWEEHAY
                     AIGCVPAGDTAEERKEII"
     gene            complement(8468..9058)
                     /locus_tag="JWH23_RS02140"
     CDS             complement(8468..9058)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS02140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407778.1"
                     /transl_table=11
                     /translation="MCEEEKLINDRHLERINRFTSIETGIYVEGELLQFCEAKMFDNEV
                     GIFLPSTFKDMKPEDAKKKYFSEQRPEIIKTNEDGTVNFSFSMVEREIKPEQLADVIQE
                     FYLVLKRFQPMSVCLEDGTESTHNVPFAWMEFISTALDDNLFNTLIIYPVGRKLLMVMF
                     SCPFEKRLDWSRCLSEIRKSITIYSKENEIGAT"
     gene            complement(9080..9550)
                     /locus_tag="JWH23_RS02145"
     CDS             complement(9080..9550)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS02145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407779.1"
                     /transl_table=11
                     /translation="MVQNKRNNESYYQYRASCSIRKEDGTEEIKNYELETDILYGDLHF
                     WNRLYYLKKSQNMLFDFASREPGIVKCRILQERLVLIPRFTYKSQKTVCRKGSLIKRLL
                     LIIEVIVLLLFVMTRASAAGEQEQGIYAKGQELSYIAGHGKENMGQYILDKD"
     gene            complement(10001..10489)
                     /locus_tag="JWH23_RS02150"
     CDS             complement(10001..10489)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="JWH23_RS02150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_277407780.1"
                     /sec_met_domain="AgrB (E-value: 6e-36, bitscore: 122.1,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQRISEDIVSWQIKKNLLKDDQRALYLYAYEVLINQIINIVVAV
                     FIAVIMRAPMPVFVFLASYIPLRSYCGGYHARTNGVCTVVSAILILIVCLLEKYIVGTV
                     ALILPIAGFLISGILIFKFAPVPDRNKLLDEEETSRYRSKKQTVLVNGSNCRCDILVC"
     gene            complement(10489..10650)
                     /locus_tag="JWH23_RS02155"
     CDS             complement(10489..10650)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342437.1"
                     /locus_tag="JWH23_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_242980208.1"
                     /sec_met_domain="TIGR04223 (E-value: 2.8e-11, bitscore:
                     41.8, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKMLNALKGKVSMVKVMNTCALLVAAYTINVTCFWAHHQPEVPE
                     EAKMLRKF"
     misc_feature    10644..10658
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.91"
                     /tool="antismash"
     gene            complement(10793..11221)
                     /locus_tag="JWH23_RS02160"
     CDS             complement(10793..11221)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="JWH23_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_349773164.1"
                     /transl_table=11
                     /translation="MQEEINIVIYSSNPVFNAILSERVSKAKELDIQLSIFIEKNLNLD
                     FISDSDMISMFGNLLDNALEAAAKCEAGKRIVNIKLFMGTNYFLIFHIENSFKVKVKKE
                     GDKLLSTKEDSKHHGLGVGIVTSLAEKYGGSLNLSQKR"
     misc_feature    11351..11365
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.5"
                     /tool="antismash"
     gene            complement(11683..12072)
                     /locus_tag="JWH23_RS02165"
     CDS             complement(11683..12072)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS02165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407781.1"
                     /transl_table=11
                     /translation="MGNDLLSINRLLFCGADVYMMCRFFAAIYPFKTFGMKRHIISLVI
                     TLLIFVENAIGSVGLNFITIPLFCYIYALLLFKVSFTNGIAYTIIYFAFVGGKEVLFEL
                     LYRFFSYNLPFYIPPWFTSGGVYFF"
     gene            complement(12074..12850)
                     /locus_tag="JWH23_RS02170"
     CDS             complement(12074..12850)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="JWH23_RS02170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_277407782.1"
                     /transl_table=11
                     /translation="MFEIAICDDSIGDRVRLTERIEKNMSESDKFRIHEYNSGISLLAA
                     IKNIRFSIIFLDVQMNEMNGEETAVRVRQLDNNVILVFYTGFAEPSPRSFEVQPYRYIM
                     KNMTDEVMDRYIRDSLDKMIELGQAPLLSANVNRERLFLRADDIVYIEKYKKNTRVHIS
                     ESSIRIYGIKDLDSSDVPDIRVSERLETIYERLKKYGFGYPHDSYIINFKYLIYCTSKI
                     LRLKDVDTMFQITRSKAQEFHMLKEEFMLSKYKDGI"
     gene            complement(13001..14746)
                     /locus_tag="JWH23_RS02175"
     CDS             complement(13001..14746)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777223.1"
                     /locus_tag="JWH23_RS02175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_277407783.1"
                     /transl_table=11
                     /translation="MNIKKHKSTIKRILNKIAKYKWSVMASLFCAFLTVVFTLYVPILI
                     GRAIDCIVGAGEVDFSAMGMILLQIAVVILITAAAQWVMSHINNQITYRVVKDIRTQAF
                     NKLEILSLKYIDSHAHGDIISRIIADIDQFSDGLLMGFTQLFTGVLTILGTLFFMFFIN
                     PVIAALVVLVTPLSLFVASFIAKRTYSMFRMQSQTRGELTSLVEEMLGNQKVVKAFAYE
                     DEAQKKFEAINEDLRIWGLKATFFSSITNPATRFVNSLVYACVGIAGAFAAVNGILSVG
                     QLSSFLSYANQYTKPFNEISGVVTELQNALASAARVFELIDEEIEVPDDSNAMVLDDAK
                     GEVVLEHVDFSYNPSVPLIEDMNLHAGQGKRIAIVGPTGCGKSTVINLLMRFYDVNAGS
                     IKVDGNDVRHITRKSLRSEYGMVLQETWLKTGTIRENIAYGKPDASEEEIITAAKEAHA
                     HSFISRMPQGYDTIISEDGGNLSQGQKQLLCIARVMLCLPPILILDEATSSIDTRTELK
                     IQKAFAKMMEGRTSFIVAHRLSTIKEADVILVMRDGHIIEQGTHEDLLSKNGFYAQLYN
                     SQFAV"
     gene            complement(14748..16478)
                     /locus_tag="JWH23_RS02180"
     CDS             complement(14748..16478)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603788.1"
                     /locus_tag="JWH23_RS02180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_277407784.1"
                     /transl_table=11
                     /translation="MKKLLKYLNEYKLESILGPLFKLLEASFELLVPLVMARVIDIGIK
                     NHDLHYILSMGGLLVLLGIIGLACSITAQYFAAKAAAGFGTSLRNDLFAHINSLSYQEI
                     DTVGTATLITRMTSDVNQVQSGVNLFLRLFLRSPFVVFGAMIMAFTIDFKAALIFVVSI
                     PVLSVVVFGIMIISMPLYKKVQKQLDRVLLTARENLGGVRVIRAFNRQNDEISRFDEEN
                     GMLVKFQVFVGKISALMNPLTYVIINLAIVVLINTGANRVESGIITQGQVVALVNYMSQ
                     ILVELVKLANLIITISKALACANRISSVFSMEPGIRETNHDIVKEEADVPKVEFDNVSL
                     FYQGAKAESLAGIEFQAMKGETIGIIGGTGSGKTTLVNLIPRFYDVSKGNVKVGGIDVK
                     NYSLSQLRNMVGIVPQRAVLFKGSIRENMQWGKRDASDEEIYRALTIAQAKDFVDEKGE
                     GLDFIIEAGGKNLSGGQRQRLTIARAVVKDPQILILDDSASALDFATDARLRKAIKEET
                     KDMTVFLVSQRASTIRNADRIVVLDDGQMAGCGKHGDLMESCEVYREICLSQLSEKEVQ
                     "
     gene            16594..17481
                     /locus_tag="JWH23_RS02185"
     CDS             16594..17481
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603787.1"
                     /locus_tag="JWH23_RS02185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_277407785.1"
                     /transl_table=11
                     /translation="MVPINSKQLYYFTVISETGSFTAAAKKLGISQPPLSKQIMLLEEE
                     LGVKLFERGAKKTELTEAGTYLYFKARDILSLMDSVSDDLKHFPSSARGLLKLGVISSA
                     GTVLVDFLKHFTADHPGVRFEVTEGNTYELLEKMKNGLVECSIIRTPFNPEGLECVYGN
                     KEPIMAVGIPEFFPEISDDKVRITGLTGKPLIYYRRFDSIISLAFQNSGIEPDIFCRND
                     DARTCLQWAKAGLGIALVPQSICDIKVQDNLMFKEIDSPDTVTQIAAVYKKNGYVSNIA
                     REFVNSFGCQNGLL"
     gene            complement(17478..18367)
                     /locus_tag="JWH23_RS02190"
                     /pseudo=""
     CDS             complement(17478..18367)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779909.1"
                     /locus_tag="JWH23_RS02190"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="radical SAM protein"
                     /pseudo=""
                     /sec_met_domain="PF04055 (E-value: 6.1e-13, bitscore: 48.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELIPAKTIVTKTKDSGWFGIDYNMNIYRGCSHGCIYCDSRSDCY
                     GIDRFDQVRVKEDALRIIRDDLRRKVKNGVVGTGAMSDPYNPLEKELELTRHALELIDA
                     FGFGAAIATKSNLLNRDIDILESIADHSPVLLKVTVTTTDDALAAKLEPRVSRPSDRLS
                     LIAALKDRGLFAGILLMPVLPFLEDHEENIRSIINAAKETGADFIYPAFGVTLRNNQRE
                     WYYDRLRELFPGEHLDENI"
     gene            18472..19302
                     /locus_tag="JWH23_RS02195"
     CDS             18472..19302
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603784.1"
                     /locus_tag="JWH23_RS02195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_277407786.1"
                     /transl_table=11
                     /translation="MIRLLLRCKQIYDKFTRDEMTVYAAQASFFTIIAAFPFIMLLLAM
                     IQFIPSITKANLLQVLITIIPSSLEIDSVIVTVIEDLYTHTPAAILSATAIAAIWSASR
                     GMLSIERGLNRVFGQENKRSYLMTRFVCACYTLVFIIICLLTLVLLVLGGSIQSFMNHH
                     FPILGEITRYVISFRTMLLFILTLCFAMLYTYVPEKKQQFMKQLPGAVFSTLGWIGFSF
                     AFSIYFSNFSNYSVMYGSLTAIVLLMLWLYSCISILFFGAEINYYYSENWKDGV"
     gene            complement(19357..20287)
                     /gene="cysK"
                     /locus_tag="JWH23_RS02200"
                     /pseudo=""
     CDS             complement(19357..20287)
                     /GO_function="GO:0004124 - cysteine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /gene="cysK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603766.1"
                     /locus_tag="JWH23_RS02200"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="cysteine synthase A"
                     /pseudo=""
                     /sec_met_domain="PALP (E-value: 5.3e-58, bitscore: 195.3,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNIKKSAMELIGNTPLLELSNYEKVHGLNACVIAKLEYFNPAGS
                     VKDRAALAMIEAGEKNGTIKPGATIIEPTSGNTGIGIASIAAMKGYRAILTMPETMSVE
                     RRNLLKAYGAEIVLTEGTKGMRGAIEKAEELQKEIEGSVIMGQFDNPANPRIHRETTGP
                     EIWADTDGKVDVFVAGIGTGGTITGVGEFLKGKNPDIRIIGVEPAGSPILSGGKPGPHG
                     IQGNWSWFCSNRTQQGCIR"
ORIGIN
        1 cgtaaaagcc cagagtaaac tgcattcttt catctggaag ttcagtaaga ggaaggctct
       61 ttggcagata aatattagcc agctcataaa ggcggacatt ttttattacg tctgttgtag
      121 tttgtagaca gtgaatttaa cattccgtga agaggagttg ttctcattac actgaagtcc
      181 tcgccaaggg gatttaaaat attcacagta acccgaagaa cactgtcctg aggaagaagg
      241 agacggtcaa aaactttagg gctttcaaag gaatatgtca ttccctgaga gaatccgcag
      301 aattctgcta cttctcttgc cagctcctcc actcttaatt tataggagag tttaccagtc
      361 gtcgcttcac cggtaggaag ggaaaccggg atcttatcat aaccataaaa tcttgctgtc
      421 tcctctgcca gatccgccat acattctaaa tcctgacgga atgtaggaac aatgatctca
      481 ccggtttttt cgtcatattc cagttccaga cgcttgtaat actcaagcat cgtctctgct
      541 gtaatatctg tacccagaag acggttcatt ctctccggtt caaatgcaac acgttttgcc
      601 tctctcttct cccggataaa tatcaatcat tccgccaact acttcacctg cgccaagctc
      661 ctcaatcagc tggcatgctc ggtcgattgc agcctttgca ttgttgggat caaggccctt
      721 ttcgaatttg ccggaagcat cggttcttaa accaactttc ttagaggaaa gacggatatt
      781 ggttccatca aagcaggcgc tttcaaatac catggtctga acttcatcgg taattttaga
      841 attttctcct cccatgatac cggcaatacc cactgccttc tccccgtcac gaatcatgag
      901 aacggtgctg tccagttttc tttcctggcc gtctaaggtc tggaaggtct ctccatcttc
      961 tgcacatctg acaacgatct catgacctgc cagctgttca taatcaaatg catgcattgg
     1021 ctgaccgaat tcttccatta catagttggt gatatcaaca atgttattga ttggtctgat
     1081 tccgcaggct gcaagtcttc tctgcatcca gacaggtgaa ggggcaatct taatattttt
     1141 accattcttg cacagtaacg ggaacaaaga cggctgtctt ctacggttac tttcagataa
     1201 tcgtgaacat cctcaccgtt gcctgtagca gttactacag gaggaacaaa ggttttgtta
     1261 aatgtagctg ctgcctctct ggcaatcccg atcacgctgt aacagtcaac acggtttgat
     1321 gttacttcat attcaaatac agaatcatga aggccaagaa gctctactgc atcacttccc
     1381 ggtacggaag tctctggaag aatatagatt ccgctttctg gagcatccgg atacatttcg
     1441 tttgtaaatc ccagttcttc aatggagcac atcattccac aggattcaat gcctcttaac
     1501 ttgccctttt tgatcttaat tccatcttcc ggaagaggac cgccgtcatg gcctcctgca
     1561 acctttccac cgtctaaaac aaccggtacc atatctcctg ttttaacgtt tgatgcgccg
     1621 gttacgatct gaacggtctc agaacctaca ttcacctggc agacaatcag tttatctgca
     1681 tccggatgac gttcaatcga taagatatga ccaactacaa tcttttctaa atttttatcc
     1741 aggcattcat agccctcaac cttcgttccg gtcagtgtca tggcatctgt atattcctga
     1801 ggtgtcactt ccaaatccgg tacatatgct ttaacccatg ataatggtgt attcatcaat
     1861 ctatctccct tcctgtttct agaactgctt taagaatctg atgtcatttt catataataa
     1921 tctcatgtca tcaatctcat attttaacaa ggcaattctc tccaagccca ctccgaatgc
     1981 aaatccggaa tattcattgg aatcgattcc gctcatggca agcacgttgg ggtgtaccat
     2041 tccgcagcct aaaatctcaa tccagccaga tcctttacag aaacggcagc cctttcctcc
     2101 acatttaaag caggtaacat ccatttccgc acttggctca gtaaacggga aatgatgggg
     2161 gcggaatttc acctttgtct caggtccaaa taactctctg gcaaattcag ccagggttcc
     2221 ttttaaatct gcaaatgtta tattcttatc gattaccagt ccctcaatct gatggaacga
     2281 aggagaatgg gttgcatcta cctcatcaga acggaacact ctgcctggtg ccagcatacg
     2341 gatgggaagc tttccctgct ccatggtacg aacctgaaca ggagaagtct gacttcttaa
     2401 aacaatgttg tcactgatat aaaaaagtat cctgctcatc tcttgccgga tgatttttcg
     2461 gaatattcag cttttcaaag ttataatggt catactctac ttccgggcct tcaatcactt
     2521 cataacccat gcccacaaag attctctcca cctcttccaa tgcgatggtg ttagggtggg
     2581 tatggccaac ccggttcctc ttcgccggca gcgtgacatc gattacttct ttcttcagct
     2641 gctcttctct tgcttttttc atcagcgcag tcatggctgc atccagctta ccttcaattg
     2701 cctctctggc ctcattcacc atctgcccaa tcttaggtct gtcttccgga gtaacgtcct
     2761 tcatgctctt taacacgctg gtcagttctc ccttttttcc gagataagca actttaatgt
     2821 cgttcagttt ctccagcgca tcggaagctt caatttgctt taacgcttct tgtttgattt
     2881 tttctaattg atctttcatc atgctctcct tcattgattc attccctgat acattaaaaa
     2941 aagctccatc cctgtatagg gacgaagctg tgttcgcggt accaccctga ttcctgcatc
     3001 gaaagcaggc actcggcata cgtaacgtgt acggacgtca tgacctacac gcctgcatat
     3061 taaataaatt cacaggtttc agccatgctg cttcagtggg aaattcgaac tcttaattga
     3121 acttaaaagg gctttcagcc ggtgacccct tctctctgat agaaattaag attctacttg
     3181 cactttcata gcatttcata ttttaatgta atattgtcac cattataata agataaaatt
     3241 aaaatgtcaa gacattttta aacctgttcc tcctataaag cggtatgaac cggtctcttt
     3301 ttaaaaataa aaaatttctg aaccagaaag ttcacaaaaa agatggagac ctccaccaca
     3361 atttttgctt ctaatccacc cattccccaa tctcttacca gccacaacag catggcggta
     3421 ttgacagcca gtatggaaag agccagcgcg gcatagccca gggcagactg caacaggctt
     3481 cctttgtaac gaaatacata attgcggttt acaaagaagt taaatgaagc gctcacaatt
     3541 cttgccatga tgttgctgac agcatctgca gttatatctc ccagcccgga agtcagggat
     3601 aaaaaaatgc caaacagcag ataatccatg cagaaactga gaaacgagga tgagacaaat
     3661 ttcaacatgg tcatataaat acgtgcagag tccttgatcg cccgaaagtg ggaagattta
     3721 ttcccttcca gatatatagt ctcaatggga atttccacta tccggatatt ctcctttaca
     3781 caggtcataa gcatatttgt ttcatactca taacggtctc ctgatatttc cagaaacatt
     3841 ggaattaagc tgctgtggaa tgctcttagt ccggtctggg tatctttcag ccattttcct
     3901 gacagcagcc agaacgtagc tcttgtaaac tgattgccca gcctgcttcg aaaaggtatc
     3961 tcgccctgaa aactgcggct gcctgtaatc agcacattcc tctctccttc tttcactccc
     4021 aataaaagag cgatatcttc cggagtatgc tgtccgtcag catctgctat gactattctt
     4081 cctgcatatt cctgattttt aataaaagac agagcggttt tcaccgctct cccttttccg
     4141 tagtttttat catggtgcaa cacaacagca aagttttctg cttcgcagaa tatctcgtct
     4201 ttttcccggc tgctcccgtc atttacaaca acaaacactg cgttcagccg ttcattcagt
     4261 tcttttaaca gatgaatcaa acgccagtcc ggctcataag ccgggatgat gatataatta
     4321 ttcttcactt ccatggtatc cctcattttt caattgtttg ataaacaact gcataggaaa
     4381 aggataccca ttatgtcgaa aaataataat atgaaaactt tattttaatt taaatccgga
     4441 aaccaccgtc ttaagctcct ctgcaatccg ctcctgcttc tctgacatct ttgacatgtc
     4501 ctgtacaact tcgtatgctt tttcaatcgt actaagaacc atgctgctgt tgcctgccgt
     4561 agtctggacg gattcggata cattctgtat ggcatcccgc acttcctgca ttacctggct
     4621 gatgttggtt gccatctcct ccacttcatt ggagatttct tcaacagtaa ctgcgtccct
     4681 gccatattgc tgacctacat tcacaaaatt gtcgtaatca ggagtcacag tatctcttaa
     4741 gaacagcagc atagactgtg atccgtctaa catcatggaa aatgcattct ggacatctgt
     4801 tatggtgccc tctatttcct ttacaatctc agaagtgtcc ttggcaagtt taccgatttc
     4861 tctggcaaca actgcgaagc ctctgccctg ttctcctgct cttaccgctt caatcgaggc
     4921 attaagtgac agaagattaa tctgggatgc cgtattggaa ataagacgag ccatatcgcc
     4981 gatccgttcc accaccttag catgctggac agccatctgg agatcctgct ctcgttcctg
     5041 tgaaatcaca atggcattgt cataagcttc cctgcttttt ttctcaatgt cttttgcccg
     5101 ttccatgatc tcgccggcca tcttgctgct cttttctgtc tcgctggcaa gtactcctac
     5161 cgatgagtta acctctgcaa gtgatgcatt cagctcttct gttgctgcac tggaagacat
     5221 catcgcctta tctacatcac tcatatatgt tttcacatcg ctgaactgaa caagcagacc
     5281 agtggatact ttatttaaat gaatgctggc atcggtaata tcacctgcct ggtcggaaat
     5341 ctgctcgatc acgcctttat tactttggaa catattgttt aatgagctgc tcatctgtcc
     5401 cagttcatct ttacgtctgg attttaacgg ctgtactgta aaatcaccat ctgcaagtga
     5461 acctgcaaac ttgcggacct tcttaaggcc tgatgtaata cttccaacct gaaacagaac
     5521 tgcaatgata cataaaacca aagctgctgc gctgatttta gccagctttt catttaaggc
     5581 aacaatgggt tctgccagtt cagattgagg catcttaatc atgatgatcc aacccacacc
     5641 aggaaccgta tcataataga ggttataagt ttctccaccc tctgtataaa ccgtctttcc
     5701 tttgtcgttt gctaccatct gggctgctgc tgcggaaagt gaggcattct catcctgagt
     5761 catattcaca cctttactca ctttttctgc atctgcgccg caaagataaa caccttcacc
     5821 ggaaattagt actgcagttc cgttcttacc aactttaatc gatttaatca gttcgtcaat
     5881 gtcgctaagc tgcatatcta ccgtaactgc tccaatgaaa cggttgctgc tgtcataaat
     5941 aggagctgtg cagcttgcca tcatcatgtt gagcgtgggg tcaaaataag gatctgttat
     6001 aattgcatcc tttgtccctc ctgatacgga tttataatac gcctgattaa aatagtcata
     6061 gctctcattg ctgtattcat tggttaaagt caccttattc ccagccttat accagtaagg
     6121 ccctacatac ttttgctggg gatcatatac atttggttca aaccagatac cgctgccaag
     6181 tattaaatca ttgccagata taatatctgc tatcgctttg ccgtatgtat caagatcagt
     6241 agactgataa gaggccccta ctatatgaga aatattcatc gctgtggtct ctaccgcatt
     6301 caaataattg tcaatgccgt ttacattcgc tttcagttct gattccatag tggaattact
     6361 ctgctgctca atcaactctc tgcttgtaga ttcactgagc ttcgctaata gtgccagtgc
     6421 cagcactacg acgggtaaaa tgccaaggag catctttatc ccaatgcttc tgattcctac
     6481 tcttcttttc ataattttat ctcctgtagt tcggacttcg acctcatact gcatgtttcg
     6541 acattttagt taaaaaacat cataacacac taaggtaaaa tttactaatt acttttgtca
     6601 atattatgta tgcctgattc cgctttttct attgcatttt tacttatgta catataaact
     6661 gattatcaga acttttatat taatacaaac tcctttcctc cctgcacttt ttacataata
     6721 ttaacagttt ttaattatac tattggtatt caaattatgc aatatatgaa ttttacatat
     6781 tttaatcgtg tattttacaa attttattat cataaaatat agtacgagag gtgactataa
     6841 tggatcagga ctatatcaat aaaagaatta cagaattacg tcttaaaaag ggagtggcag
     6901 aacataagat gagcctggag cttgggcaca gcagaagtta cgtacaaagt atctccagtg
     6961 gcagaagtct cccttccatg gcggagtttc tggcaatctg tgagtactta agcgtaacgc
     7021 cgaaagattt tttcgataat gataatccca acccgccctt agtcagggat acaatagaca
     7081 agcttaaatc tttgccggaa agtgatttac tgctgatctt aaccatgatc aaccggcttc
     7141 agtcaaaata ggcgcaaaca aatcttttat aacagatcat ttcattgtcc ccgtgcagaa
     7201 gtacggggat tttgaggtac aaagggacgc gctgtttagt tttttaaact gctgacacct
     7261 gatgattttt aacatcattg atctcatcca gggaaacaat ggagggaatt gcctccaaag
     7321 cccttacata taattcatcc agatctcctc tgtttatcat tccaaaaaaa tatccgtata
     7381 gatcctgaaa cgtctgatct tttgtcatat aatctccttc ctttcttcag ccgtatctcc
     7441 ggccggaacg caaccaattg catacgcatg ttcttcccag ccatctggta aatataaccg
     7501 aatgggatct cctggtctgg gcgtaacata caagcccatt ccttccggtg acgtatatac
     7561 agaagaaaac ggaaagcaaa tgggctttcc ataggtttca tctttctcac tcagcagctt
     7621 tatctgaacc gcgttctctc ctgtattttt aacattcccc atgatcataa gtcctgcaag
     7681 acttagatgc tccttggccg gaaccagaaa acccgaccgg gtgcggagta cataatcaaa
     7741 ttccacctta cctccgctga aattcatact catggagtat atgtaataag cgctgtcttc
     7801 tatcatcaca caatcacata agtgcaggca ctttctgctt ttaatcagat attcaacagt
     7861 ctcacctttt tcatagtcat cgtaacattt aattcggtac tggtcagaat taaaacactt
     7921 tctgcctttt ctttttattc ttccaaaatg aaaacagatg ttattatgaa cactgtctgc
     7981 caccagaatc tgatttttct ttgcagcaag acgtattaaa aactgccagt ctgtctccat
     8041 atactgtaca gccaggccat cagacgtttc ccgcatttct ttacttaaaa ccacagtcgt
     8101 attctcatat ccgtcaagca ccgatccaat tatctgtaga taattctgtc cctcaccctg
     8161 aaacgtcctt atatgctttt ctctgtcaag atccatagtc agggacactg cgaacagatt
     8221 ccaataaaca cttccatccc cctcaatttc gtatcttctt tcgattctgc ctttgaaaat
     8281 gggtatttct ttcttttcct cattcacaaa agttatagtc agcaaaacct cctttgattc
     8341 tctaagtatc agattatctt tatctgaatt taccactccg gttacaacag ctgtaaaatg
     8401 ttgatttact cttttatcaa taaaacattg tgtcggataa aacatctccc cggaatccaa
     8461 atagattcta agttgctcca atttcattct ccttactata tatcgtaata ctcttgcgga
     8521 tttcgcttaa gcagcggctc cagtccagcc ttttctcaaa cggacagcta aacatcacca
     8581 tcagcagctt tctgcccacc gggtaaataa tcaatgtatt aaatagatta tcatcaagag
     8641 ctgtactgat aaattccatc catgcaaaag gcacattatg ggttgactcc gtcccatctt
     8701 ccagacaaac actcattggt tgaaatcgct ttaaaacaag ataaaattcc tgtattacat
     8761 ccgcaagctg ttctggctta atctctcttt ctaccatact gaaggagaaa ttaacagtgc
     8821 catcttcatt tgtttttata atttcaggac gctgctcaga aaaatacttt ttctttgcat
     8881 cctccggttt catatcctta aaggtggagg gaagaaaaat gcccacttcg ttatcaaaca
     8941 tctttgcttc acaaaactga agcaattctc cctccacata aatccctgtt tcaatactgg
     9001 taaaccggtt aatccgttca agatgcctgt cattaatcag tttctcttct tcacacatga
     9061 ttcttcctct ctggtttgtt caatctttat ctaatatata ctgccccatg ttctcctttc
     9121 catggcctgc aatataagac agttcctgac cctttgcgta aataccctgt tcttgctctc
     9181 ctgcagcaga tgctctggtc ataacaaaaa gcagtaatac aatcacttca attatgagaa
     9241 gcagtctttt gattaatgat ccttttctgc atacagtctt ttgcgattta taagtaaatc
     9301 ttggtattaa aaccagcctt tcctgtaaga ttctgcattt tacaattcct ggttcacggg
     9361 atgcaaagtc aaacagcata ttctgacttt ttttcagata atataaacga ttccaaaaat
     9421 ggagatctcc gtataaaata tctgtttcca attcgtaatt ttttatttcc tctgttccat
     9481 cctctttacg gattgagcag cttgctctgt attggtaata gctttcattg tttcttttat
     9541 tctgcaccat cagccctcct ttcgttccga gaactatatc acttatattt acattctaca
     9601 tataactgaa agaaattcaa ccgaagtgct aaaattgtac acattgagct ccaaaatttg
     9661 catgcgggac ctgttgaaac agctttttat ttaagaatag taagaaatat tatgcttata
     9721 atgttctatt taagggattg agaagataga agctgaggcg gcattgagca ccgtctcaat
     9781 tgaccagatt tcagaattat aatgataata taagaaatac aaaaaagaga atgatgtctt
     9841 agggattgga attcgaatca aatctataat agaaaaacga gactgctgcc aggcaacagt
     9901 ctcgttttcc tagttcactt tactattctt cagcattcca tatacaagca taacggacag
     9961 cagcatatgg cttattgcca gtacaacaca gactctcata ttaacaaacc agaatatcgc
    10021 acctacaatt acttccgtta accagaactg tctgcttttt tgaccggtaa cggcttgtct
    10081 cctcttcgtc gagaagcttg ttcctgtcag gaacaggtgc aaacttaaag atcagaattc
    10141 cagaaataag aaaccctgca attggcaata taagagccac tgtcccgact atgtacttct
    10201 caagcagaca aacaatcaga ataaggattg ctgaaactac agtacagact ccattggttc
    10261 ttgcgtgata cccgccgcaa tatgacctta atggtatata gctggcaagg aacacaaaca
    10321 ctggcatagg tgcacgcatg attaccgcta tgaacacagc cacgactatg tttataatct
    10381 ggttaattaa tacctcatag gcgtaaagat ataaagcgcg ctgatcatct tttaaaagat
    10441 tctttttaat ctgccagctg actatgtctt ctgaaatccg ttgtaacatt agaacttcct
    10501 gagcatctta gcctcttctg gcacttctgg ctgatgatga gcccagaaac aggttacatt
    10561 aattgtataa gctgcaacca gtaatgcgca tgtgttcatc accttcacca ttgatacttt
    10621 tccttttaaa gcatttaaca tttttctcat tttgagacct ccttttttct ttcataatat
    10681 aacagtaatt tacgtttttc aagtcaagtg ccaaaattat acacgttgag tgccaaaatt
    10741 tgcacacagc ccttacgaaa acgcagaaat cgttaatact gtatcgaata aattatcttt
    10801 tttgagaaag atttaatgac cctccatact tctctgccaa cgatgttacg attcctactc
    10861 ccaaaccatg atgttttgaa tcttcttttg ttgacagcag tttatccccc tcctttttga
    10921 ctttcacttt aaaactattt tcaatatgga atatcagaaa ataattagtt cccataaata
    10981 gttttatatt tacaattcgc tttcccgcct cacacttcgc agctgcctcc agcgcattgt
    11041 caagcagatt accaaacatt gaaatcatat cggaatctga tatgaaatcc agatttaaat
    11101 ttttttctat aaatatagat aactgtatat caagttcctt tgcctttgaa acacgttcag
    11161 ataaaatagc attaaatact ggattgctgc tataaattac aatattaatc tcttcttgta
    11221 tttttaccct ttaattcatc aataatttca actattttat tattttcccc atttaaagct
    11281 aataaacgaa agctgttaaa ataagagtga acatcatgca taaagcagcg gtaatggtca
    11341 tttattttaa aatattctga aaatgatcat tttccatctc tcttttaacc gaataaagtt
    11401 ctgcatattt tattttattc atcatatccg tatatctctc caggatgata aatatagctg
    11461 catttgatag atataacaac accgctccac cgcacataaa aaattgcaaa acaatagtct
    11521 taggaaaatc catatataaa aaagctgctt gaaatagcaa gggataaaat tggcagaatc
    11581 agtaaatacc acgaaaatcc attattattc cttatgtttt tttttttaat atacttttca
    11641 atataaatta aaaaaataga atacctacaa tatattctaa tattaaaaaa aatatactcc
    11701 acctgatgtg aaccatggag gtatataaaa aggcagatta tatgaaaaga aacgatataa
    11761 tagttcaaaa agaacttctt ttcctcccac aaaagcaaaa taaattatgg tataagcaat
    11821 tccatttgta aatgaaactt taaataatag caaagcataa atataacaaa aaagtggaat
    11881 tgtaataaaa ttcaggccca cactgccaat tgcattttca acaaaaatca ataatgttat
    11941 aaccaaacta attatatgac gcttcatacc aaaagtttta aaaggatata tggcggcaaa
    12001 aaatctgcac atcatataaa catctgcacc acaaaataaa agcctgttta ttgacaacaa
    12061 atcatttccc atatcaaatg ccatccttat attttgataa cataaattct tctttcagca
    12121 tatgaaactc ctgcgccttg cttctggtta tctgaaacat ggtatcaaca tccttcagcc
    12181 gcaatatctt actggtgcag tagatcaaat atttaaaatt tataatatag ctgtcgtgtg
    12241 gatagccaaa accatatttt ttaaggcgct cgtaaattgt ttccagtctt tctgaaacgc
    12301 ggatatccgg aacgtcactg ctatctaaat cttttattcc atagattctg attgaacttt
    12361 cagagatatg gactctggtg tttttcttat atttttcaat gtaaacaata tcatctgctc
    12421 ttagaaacag ccgctcccgg tttacattgg cagaaagcag gggtgcctgt cccaattcga
    12481 tcattttatc aagagaatcc cttatgtaac gatccatgac ttcatcagtc atattcttca
    12541 taatataacg atatggctga acttcaaaac tacgcggcga aggttccgca aaacccgtat
    12601 aaaaaaccag tatcacatta ttatccagct gacgaaccct gactgccgtt tcttcaccat
    12661 tcatttcgtt catctgaaca tcaagaaata taatagaaaa tctgatgttc tttatcgctg
    12721 ccaaaaggct gattcctgaa ttgtattcat ggattcggaa tttatcgctt tccgacatgt
    12781 ttttttcaat tcgttctgtt aaacgcactc tgtcgccgat actatcatca caaatagcaa
    12841 tttcaaacat actttttccc ctcttactaa atttctttct attagatatc gtactatctc
    12901 tcctatttta taagaaatat gtcaataata caagaaaagg taccagaaaa atacaaattt
    12961 catattttcc cggtgcctta tttgtaatta tcctgccgaa ttataccgca aactggctat
    13021 tgtaaagctg agcataaaaa ccatttttag aaagaagatc ctcatgagtt ccctgttcaa
    13081 taatatgacc atctctcatg acaagtatga catctgcctc tttgattgtg gaaagacgat
    13141 gtgcaacaat aaaactggtg cgcccttcca tcattttcgc aaaagccttt tgtatcttta
    13201 attccgtcct tgtatcaatg gatgaagtgg cttcatcaag aatgagaatt ggcggcaggc
    13261 atagcataac tctggcaatg cacaaaagct gcttctgtcc ctgggataaa tttcctccgt
    13321 cctctgatat gattgtatca tatccctgag gcattcgtga aataaagctg tgggcatggg
    13381 cttcttttgc cgctgtaata atttcttctt ccgaagcgtc cggcttcccg tatgcaatat
    13441 tttctcttat ggtacctgtc tttaaccagg tttcctgaag aaccattcca tattcagatc
    13501 ttaaactttt tctggttatg tgccgcacat cgtttccatc aacctttatc gatcctgcat
    13561 tgacatcgta aaaacgcata agcaggttta taacagtact ttttccgcag cctgtgggac
    13621 caacaatggc aatcctcttt ccctgccctg catgaagatt catatcttca atcaaaggta
    13681 cactgggatt gtaggagaag tcaacatgct ccagtacaac ctcacccttt gcatcatcca
    13741 gtaccatcgc attgctgtca tctggtactt ctatttcttc atcaattaat tcaaaaaccc
    13801 tggcagcaga tgcaagtgcg ttctgaagct ctgttaccac tcctgaaatc tcattaaacg
    13861 gctttgtata ctgattggca taacttagaa agctggacag ctgccccact gataaaatgc
    13921 cattgactgc ggcaaatgct cctgcaattc ctacacacgc atatacaagg ctgtttacaa
    13981 accgggtcgc cgggtttgtg atggaagaga aaaatgtggc tttcagcccc catatacgca
    14041 ggtcttcatt gatggcttca aatttcttct gggcttcgtc ttcataagca aaggctttca
    14101 ccactttctg attgcctaac atctcttcca ccagtgaggt cagctctcct ctggtctgag
    14161 actgcatccg aaacatgctg taagttcttt ttgcaataaa gctggccaca aacagggaca
    14221 gcggtgtaac tagcacaacc agggcagcaa tcaccggatt tataaaaaac ataaagaata
    14281 atgttccaag gattgttaaa actccagtga aaagctgggt aaatcccatg agcagaccgt
    14341 ctgaaaactg atcaatatca gcaatgattc tgctgatgat atcaccatgt gcatgggaat
    14401 caatatactt taaagaaaga atctccagtt tgttaaatgc ctgagttctg atatccttta
    14461 ctacacggta ggtgatctga ttgttaatat ggctcatcac ccactgtgct gcagcagtaa
    14521 tcagtataac tacagcaatt tgcaaaagta tcattcccat ggcgctaaag tctacttctc
    14581 ctgcccctac aatacagtca attgcacgtc ctattaatat gggaacatac aaggtgaaaa
    14641 ccacggtaag aaatgcgcag aacagagatg ccataacact ccatttatac tttgcaatct
    14701 tatttaaaat ccgcttaatt gtggacttgt gcttttttat gttcatatta ctgcacctcc
    14761 ttttcagaca gctgggaaag gcagatttca cggtagactt cacaggattc cattaaatca
    14821 ccatgctttc cgcagcctgc catctgcccg tcgtctagaa ccacgattcg gtcagcattt
    14881 ctgattgtgg aagctctctg ggataccaga aatactgtca tgtcttttgt ttcttcttta
    14941 attgctttac gaaggcgggc atctgtggca aaatccagag cggaagcgct gtcatctaaa
    15001 ataagaatct gaggatcttt gacaactgct ctggctatgg taagacgctg acgctgtcct
    15061 ccggataagt tcttacctcc tgcctctata ataaagtcaa gaccttcacc cttctcatcc
    15121 acaaaatcct tagcctgggc aatggtaaga gcacggtaaa tctcttcgtc agaagcatct
    15181 ctttttcccc actgcatgtt ttccctgatg cttcctttaa ataatacggc tctttgagga
    15241 acgattccaa ccatatttct cagctggctg agagaataat ttttaacatc aattcctccc
    15301 actttaacat ttcccttaga tacatcataa aatcggggaa ttaaatttac cagtgtggtt
    15361 ttacccgaac cggttccgcc tataattcca attgtttcac ctttcatagc ctgaaattca
    15421 attccggcca gtgattctgc ttttgcaccc tgataaaaaa gactgacatt atcaaattcc
    15481 accttgggta catctgcttc ttctttcaca atatcgtgat ttgtctccct gattcccggt
    15541 tccatggaaa atacggaact gatccggttg gcacaggcca gcgcttttga tatggtgata
    15601 attaagtttg ccagtttcac cagttccacc agaatctgag acatataatt caccagagcc
    15661 accacctgac cctgggtaat aatacccgat tcaacccggt ttgcaccagt gttaatcaaa
    15721 actacaatcg caaggttaat tatgacataa gtaagaggat tcatcagtgc agagatcttt
    15781 cctacaaaca cctgaaattt taccagcatg ccattttctt catcaaaccg gctgatttca
    15841 tcattctggc ggttaaaggc gcgaattaca cggactccgc ctaaattttc tcttgcagtc
    15901 agaagaaccc ggtccagctg cttttgtacc ttcttatata aaggcatact tataatcata
    15961 atgccaaata ctacaacaga aagtacaggt atggaaacca caaatataag agcagcctta
    16021 aaatctatgg tgaatgccat gatcattgca ccgaatacca cgaaggggga acggagaaac
    16081 aaccgcaaaa acagattgac ccctgattga acctgattca catcgctggt cattcgtgta
    16141 atcagtgtgg cagtccctac ggtatcaatt tcctgataag acaagctgtt gatatgggca
    16201 aataaatcat ttctcagtga ggtaccaaat ccggcagctg cctttgccgc gaagtactgg
    16261 gcagtaatgg agcaggcaag ccctataatg cctaacagca ccagcaatcc gcccatagaa
    16321 agaatatagt gcagatcgtg attcttaatt ccaatatcaa tgactctggc cataaccaaa
    16381 ggcactaaaa gctcaaagct ggcctccaga agtttaaaca aaggccccag aatactttcc
    16441 agcttatact catttagata tttaaggagc tttttcattt tccttttcct ccgtaatcaa
    16501 tactttttta gaataatgcc ttgtattatt ataccatttt gatataataa tacaagtact
    16561 aattgaaaat cttatgaata gaggatgtac catatggttc cgattaattc aaaacagctt
    16621 tattatttta cagtcatttc agaaaccggc agttttacag ctgccgcaaa gaaactggga
    16681 atatcccagc cgcctttaag caagcagatc atgcttttgg aagaggagtt gggagttaaa
    16741 ctttttgaac ggggagcgaa gaaaacagaa cttacagaag caggcactta tttatatttt
    16801 aaggcaagag atattttgtc tctgatggac tccgtatcag atgatttaaa acacttcccc
    16861 tcttctgcca gaggcctttt aaagctgggt gtcatctcct cagcgggaac agttcttgtg
    16921 gattttctga aacattttac agctgatcat ccaggtgtcc ggtttgaagt gacagaagga
    16981 aacacctatg agctgctgga gaagatgaaa aacggactgg ttgagtgttc tattattagg
    17041 acacctttta atccggaagg tctggaatgt gtctatggta acaaagaacc gattatggca
    17101 gtgggaattc cagaattttt tccggagata tcagatgata aagtaagaat taccggatta
    17161 acgggaaagc ctctgattta ttaccgcaga tttgattcca tcatatctct tgcatttcaa
    17221 aacagcggca tagaaccgga tatattctgc cgtaatgacg atgccagaac ctgtcttcaa
    17281 tgggcaaaag ccggacttgg gatcgcactg gtgcctcaat ccatctgtga tattaaggtg
    17341 caggataacc ttatgtttaa ggagattgat tctcctgata cagtcactca gattgctgca
    17401 gtttataaaa aaaatggcta tgtttcaaac atagccagag aatttgtaaa tagttttggt
    17461 tgtcagaacg gcttgcttta atcaaacagg cttaactggg ttatctggta ttttttctta
    17521 taagcatgaa tgatatccgg cattttatac agaatcctgt tgcgctcgca ctcttcagaa
    17581 aatatttgcc ataatttgcg ggctttagga ctggtacaat gataattgtt cttatactgt
    17641 ttcatatatt ttcgtcaaga tgttccccgg gaaacaactc ccttaagcgg tcgtaatacc
    17701 attctctttg attgttacga agtgtaaccc caaaagccgg atagataaaa tcggcaccgg
    17761 tttcttttgc ggcatttatg attgaacgta tattctcctc atgatcctct aaaaaaggaa
    17821 gcactggcat gaggagaatc cctgcaaaca gcccccgatc ctttaacgcc gcaatcagag
    17881 ataaccggtc agaaggtctg gatactcttg gttctaattt agccgccagg gcatcatcag
    17941 tggttgtaac cgtaactttt aacagtacag gagaatgatc tgctatactt tccagtatat
    18001 caatgtctct gttaagcaga ttgctttttg tggcaatcgc tgcgccaaag ccaaaggcat
    18061 caattaattc cagagcatgt ctggtaagtt ccaattcctt ttccaatgga ttatagggat
    18121 cactcatagc cccagtgcca accactccat tttttacctt tcgtcttaaa tcatcccgga
    18181 taattcgcag ggcatcctct ttgacccgaa cctgatcaaa ccggtctatt ccatagcagt
    18241 ccgaacggct gtcacagtaa atacacccat ggctgcagcc cctgtaaata ttcatattat
    18301 aatcaatccc gaaccacccg gaatcctttg tttttgttac aattgttttt gcaggaatga
    18361 gttccatgca tccgtcctct ctttcttttt ctgccaaaaa aggtgatccc ttgatatttt
    18421 ctttctgcca tgttaatatg cttatacgag ataagaaggg aagagtttat tatgatacgt
    18481 ttgttattgc gctgcaagca gatatacgac aaatttacca gagatgaaat gactgtctat
    18541 gcagcacagg catcattttt taccataatt gctgcttttc cgtttattat gctgttatta
    18601 gcaatgattc agtttatccc atctattaca aaagcgaacc tgctccaggt tttaatcaca
    18661 atcattccaa gctccttaga gattgacagc gtcatcgtta ccgtaattga agatctttat
    18721 actcatactc ctgcggcaat tttatctgcc actgccattg ccgctatctg gtccgcttcc
    18781 agaggcatgt tgagcataga gcgtggattg aaccgtgttt tcggccagga gaacaagcgg
    18841 agctatctaa tgacccgttt tgtctgtgcc tgttatacgc ttgtattcat cattatctgt
    18901 cttctgacgc ttgtacttct ggtactggga ggttccatac agtcatttat gaaccaccat
    18961 tttcctatcc ttggtgagat cacccgatat gttatcagct ttcgcaccat gcttttattt
    19021 attctcacct tgtgttttgc catgctttat acctatgtac cagaaaagaa acagcaattt
    19081 atgaagcagc tcccgggtgc cgtcttttct acccttggct ggatcggttt ctcctttgct
    19141 ttttctatct attttagtaa tttcagcaac tattcagtga tgtacggaag cctgactgcc
    19201 atcgtccttt taatgttatg gctttattcc tgcatctcca tcctgttttt cggagccgaa
    19261 atcaattatt attattcaga gaactggaaa gatggagtat aatttaggaa agccagtgat
    19321 tcccttaaga atccctggct ttttgatacg ggcgatctaa gaaaaaaggg gcgttgacaa
    19381 atatctgtca cctgtgtcag gaagaagcac tacgattgtt ttaccttgga attcaggccg
    19441 tttggctact tcttttgcag cccatacagc tgcggcagag gagataccca cgagaattcc
    19501 ttctgtctta gcaatctctt ttccggtttc atatgcatcg ttgttctcta ccggtataat
    19561 ttcatcgtat acatccttgt tgagtacggt tggaacaaaa ccagctccaa ttcccctgga
    19621 ttccatgagg gcctggcttt ccgcctgaaa gaatgggtga tcctgccggt tccactccaa
    19681 taatcctgat atccgggttc ttgcctttta aaaattcccc aacaccagtg attgttcctc
    19741 cagtaccaat tcctgcaaca aatacatcga cttttccatc tgtatccgcc catatttcag
    19801 gtccggttgt ttcacggtgt attcttggat tagcaggatt atcaaactgt cccatgataa
    19861 ctgaaccctc tatctccttt tgcagctctt ctgctttttc aattgcgcct ctcattcctt
    19921 ttgttccctc tgtaagcaca atttcagccc cgtacgcttt taaaagattc cttctttcta
    19981 cactcatggt ctctggcata gtaaggattg cgcggtagcc tttcatggca gcaatgctgg
    20041 caattccgat cccggtattc ccggaagtag gttcaataat agtggcacct ggtttaattg
    20101 taccattttt ttctccagcc tcaatcatgg ctaatgctgc tctgtctttc acactgcctg
    20161 ccggattaaa atattccagc tttgcaatga cacaagcatt cagcccatgc actttttcat
    20221 aattggaaag ttccagcaaa ggagtattgc caatgagttc catagcactt tttttgatat
    20281 ttcccataat caacctcttt tctcatacgt ttatagatcc cgcgtatgtg tcctgattat
    20341 attttatctg caggcatgta ttattatgat aaaccgtaaa tcttgcggta cttttctttc
    20401 aggtaatcaa tgaagtattc cggagtaaag tcctccccgg ttgccgcctt tagaatctca
    20461 cggcttgtct tcatctttcc gtactgatga acatggcttc gcagatattc tttgatcaca
    20521 tccatcttcc cgtctcttaa aagctgatca aaatccattt ccttcttcat atggaaataa
    20581 agctgtgctc caaaggcatt gcctaatgca taggagggga aataaccaaa aagtccctgt
    20641 gaccagtgaa
//
