LOCUS       NZ_CP070896            20471 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Lacrimispora xylanisolvens strain ASCUSBR21 chromosome, complete
            genome.
ACCESSION   NZ_CP070896
VERSION     NZ_CP070896.1
KEYWORDS    RefSeq.
SOURCE      Lacrimispora xylanisolvens
  ORGANISM  Lacrimispora xylanisolvens
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Lacrimispora.
REFERENCE   1  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Hungatella xylanolytica ASCUSBR21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633035)
  AUTHORS   Gaffney,J.R. and Embree,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) R&D, Ascus Biosciences, 6450 Lusk Blvd E209,
            San Diego, CA 92121, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAY-2019
            Assembly Method                   :: Canu v. 2.1.1
            Assembly Name :: Hungatella xylanolytica ASCUSBR21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017084465.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,443
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 686
            CDSs (without protein)            :: 686
            Pseudo Genes (ambiguous residues) :: 0 of 686
            Pseudo Genes (frameshifted)       :: 599 of 686
            Pseudo Genes (incomplete)         :: 95 of 686
            Pseudo Genes (internal stop)      :: 24 of 686
            Pseudo Genes (multiple problems)  :: 30 of 686
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:11:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 29728
            Orig. end                         :: 50199
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20471
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20471
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20471
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10471](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10471
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            740..970
                     /gene="acpP"
                     /locus_tag="JWH23_RS00200"
     CDS             740..970
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (3-69). E-value: 8.5e-17.
                     Score: 53.2. Matches aSDomain:
                     nrpspksdomains_JWH23_RS00200_PP-binding.1"
                     /codon_start=1
                     /gene="acpP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725969.1"
                     /locus_tag="JWH23_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_104436156.1"
                     /sec_met_domain="PP-binding (E-value: 6.2e-15, bitscore:
                     54.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding_2 (E-value: 0.00014, bitscore:
                     20.5, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFEKLQKIIAEVLNIEPEEVTMNSTFVDDLGADSLDVFQIVMGI
                     EEEFDIEIPTEVAENIVSVSDAVEQVKNALN"
     aSDomain        749..946
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JWH23_RS00200_PP-binding.1"
                     /evalue="8.50E-17"
                     /label="JWH23_RS00200_PP-binding.1"
                     /locus_tag="JWH23_RS00200"
                     /protein_end="69"
                     /protein_start="3"
                     /score="53.2"
                     /tool="antismash"
                     /translation="EKLQKIIAEVLNIEPEEVTMNSTFVDDLGADSLDVFQIVMGIEEE
                     FDIEIPTEVAENIVSVSDAVE"
     misc_feature    complement(752..766)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.21"
                     /tool="antismash"
     gene            1150..1840
                     /gene="rnc"
                     /locus_tag="JWH23_RS00205"
                     /pseudo=""
     CDS             1150..1840
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0004525 - ribonuclease III activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnc"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865052.1"
                     /locus_tag="JWH23_RS00205"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ribonuclease III"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSRSMKELEERIGYRFSDKHLMTQAMTHSSYANEHRLNKTECNER
                     LEFLGDSVLEVVSSDCLYHKYPQKPEGDLTKKFVPALFANLPLLIVQK"
     misc_feature    complement(1967..1982)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
     gene            2040..2147
                     /locus_tag="JWH23_RS00210"
     CDS             2040..2147
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /locus_tag="JWH23_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_277410019.1"
                     /transl_table=11
                     /translation="MVFEFHNGITGIVGPNGSGKSNVADAVRWVLGEQK"
     gene            2134..3207
                     /locus_tag="JWH23_RS00215"
     CDS             2134..3207
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407525.1"
                     /transl_table=11
                     /translation="MNKSKAVKKFQYAGCYFFSGTEMRKPQGFAYVAITLDNSDHHLSI
                     DYDQVTVSRRVYRSGESEYMINGSACRLKDINELFYDTGIGKEGYSIIGQGQIDKILSG
                     KPEERRELFDEAAGIVKFKRRKAIAQKKLEDEKQNLVRVSDILSELEKQVGPLAKQSEA
                     AKEYLRLKEELKKYDVNQFLMETEGLRFQMKEVDEKEVIVSRDLEEAKNASEGIREQYE
                     TLDTYLSQLDESILVSRNEKNNSDIETGNLQGRINVLKEQINTEQMNAEHIAGRVRSIH
                     SEIDMKKSQASSYEEERSVIAESVKTAMEQLKQGETVLSAEDEKIHLLEQRIESGKGSI
                     IELLNEKSHLKCQTAAL"
     gene            3131..5551
                     /gene="smc"
                     /locus_tag="JWH23_RS00220"
     CDS             3131..5551
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="smc"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /locus_tag="JWH23_RS00220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chromosome segregation protein SMC"
                     /protein_id="WP_349772803.1"
                     /transl_table=11
                     /translation="MNLVRGALLSFSMKKATLNAKQQRYETMLEQVNVRRSEVLQKLLK
                     YKSDESEQDQQLLVLQKEADEIDAGIFKYNELQEQSESRAGELEHEVKRLGKNLNDRQQ
                     EYHTNSTKLESLRNLAERYEGYGGSIRRVMEVKDRVHGIHGVVADLITVSKKYEVAIET
                     ALGGSIQNVVTDSDQTAKQLIEYLKKNKYGRATFLPLTSIGNKNGFNQEKALSEPGVLG
                     LANSLVEADSQYQGLLNYLLGRVVVVDTIDHAIALAKKFQYSLRIVTLEGELLSAGGSM
                     TGGAFKNTSNLLGRKREMEELEAACEKALKETGQIETELVMNEGLLSECKEELETIRSE
                     KQKLYLRQNTVKINLRRIEDKKAEIIESYGDLELENNQLEVQKKEIGENRQELILAVEK
                     LAVRSQDIDGDLESLNADLEKAKADREKFSRELSEAQLAASSLKQKDDFELENIRRVKD
                     EISRLEEELFKLSQGSSGSGLIIEEKQKEIESIKARIAEISSARETLDEAINEKLAKKE
                     MKSKEQKELFQKREEFTQRISLLDKELFRLQSQKEKMEERMDNHVNYMWNEYELTFSKA
                     EALRNEEWTSLPDIKKMIGSLKEEIRKLGNVNVNAIEDYKEVSERYEFMKTQHDDLITA
                     EGTLLKIIDELDTGMRKQFEEKFREIRVEFDKVFKELFGGGRGALELVEDEDILEAGIQ
                     IVSQPPGKKLQNMMQLSGGEKALTAIALLFAIQNLKPSPFCLLDEIEAALDDSNVDRFA
                     KYLHKLTKFTQFIVITHRRGTMVSADRLYGITMQEKGVSTLVSVNLIEEDLES"
     gene            5563..6503
                     /gene="ftsY"
                     /locus_tag="JWH23_RS00225"
                     /pseudo=""
     CDS             5563..6503
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005047 - signal recognition particle
                     binding [Evidence IEA]"
                     /GO_process="GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="ftsY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775637.1"
                     /locus_tag="JWH23_RS00225"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="signal recognition particle-docking protein FtsY"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGEKKGFFGKLVAGLQKTRDNIIAGVDSIFNGFSAIDDEFYEEIE
                     EILIMGDLGIQTTTSIVEDLRKK"
     gene            6507..7730
                     /gene="ilvA"
                     /locus_tag="JWH23_RS00230"
     CDS             6507..7730
                     /EC_number="4.3.1.19"
                     /GO_function="GO:0004794 - threonine deaminase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ilvA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604196.1"
                     /locus_tag="JWH23_RS00230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine ammonia-lyase"
                     /protein_id="WP_277407527.1"
                     /sec_met_domain="PALP (E-value: 5.9e-77, bitscore: 257.5,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTLEKFEEASEVVGKVTLETKLIYSEYFSAQTGNKVYFKPENMQ
                     YTGAYKVRGAYYKISTLTEEEKSKGLITASAGNHAQGVAYAAKLAGIPATIVMPTTTPL
                     MKVNRTKGYGAEVILEGDVFDEACNHAYKLAEERGLTFVHPFNDLDVATGQGTIAMEII
                     KELPTVDYILVPIGGGGLCTGVSVLAKMLNPKIKVIGVEPAGANCMQESIRQGKVVSLP
                     TVNTIADGTAVKCPGDKLFPYIQENVDSIITIEDSELIVAFLDMMENHKMVVENSGLLT
                     VAALKHIDVDNKKIVSILSGGNMDVITMASIVQLGLIQRDRIFTVSILLPDKPGELAKV
                     SALLAKEQGNVIRLEHNQFISINRNTAVELRITLEAFGTDHKNAIMAALTSQGYRPKLV
                     KSKGTYGD"
     gene            complement(7785..8525)
                     /locus_tag="JWH23_RS00235"
     CDS             complement(7785..8525)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="JWH23_RS00235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_277407528.1"
                     /transl_table=11
                     /translation="MIHIYLCEDDKRQLLRWTSIIEKYLLMNSTESLLYCATSYPDDLL
                     KVRKDSNITGLYFLDIDLRANKNGIQLAEEIRKYDPRGYIVFVTTHSEMAYLTFQYKVE
                     AMDFIVKDKVSDLPEQIYSCIKNAETKYRNQLDSSDRLLSIKVDKASLVMDQDDIVAIT
                     TSEEYHKIIIHTLNGFRQMSGSLKELSASLNSHFCQCNRSTIVNLKHVIKYSREDALLY
                     MNNKESYSVSVRLLSKVHKSLKCL"
     gene            complement(8522..9454)
                     /locus_tag="JWH23_RS00240"
     CDS             complement(8522..9454)
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="JWH23_RS00240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_277407529.1"
                     /transl_table=11
                     /translation="MLGWYFHKKLNLSKYLTNRHLLKAIFTDLFILVFFYIFNFSYGEQ
                     LGYNYGVIALNGILFLLLFAITVFLMYSIYKATMGELSYKNRMSQFENLRTYTDRLEMS
                     YGTMRKFKHDYMNILITMSGFMEDNDLQGLKKYFGEKIIPISHSFTESDTKLGTLSNIK
                     DTALKSLLSSKFIYSMETGIKAEIELTEPITNLYMDALDLSRIIGIFLDNAIEATAETN
                     EKELHFCMFYKEDDLYIVIKNTALPPDHSLYKLREQGVSTKGENRGVGLYNVSMVLNHY
                     DNVVWNTIYKEPYFTQELILMHYGNKEQL"
     misc_feature    9700..9713
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.11"
                     /tool="antismash"
     misc_feature    9784..9795
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 23.05"
                     /tool="antismash"
     gene            10001..10471
                     /locus_tag="JWH23_RS00245"
     CDS             10001..10471
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="JWH23_RS00245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_277407530.1"
                     /sec_met_domain="AgrB (E-value: 4.4e-24, bitscore: 83.4,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALFGGDKVIISRFSAALCCLLNKVNPRDEDDNITIQYGVELILD
                     NLLKLLFIQLLGFLFGMGVETFIILFVFCSLRLQAGGIHAKTNAGCFFGMVTVWVLSLS
                     GTIVFKLSIFNVLVIYVFSLIIFICLVPKSKNITYFTRSAILKKKNYINFFC"
     gene            10594..10743
                     /locus_tag="JWH23_RS00250"
     CDS             10594..10743
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS00250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="WP_277407531.1"
                     /transl_table=11
                     /translation="MKKEIVKGLQKVLLKVSVNSVEKSIPATMYEREIPKAVLKKHETE
                     AKNN"
     gene            10939..11127
                     /locus_tag="JWH23_RS00255"
     CDS             10939..11127
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS00255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407532.1"
                     /transl_table=11
                     /translation="MKTKMFRKFLGLEREIPHYSVLNDDTSSDSKRMIIPREYLIEVIT
                     TIPERRVVIEHLDVYQE"
     gene            complement(11196..12941)
                     /locus_tag="JWH23_RS00260"
     CDS             complement(11196..12941)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780434.1"
                     /locus_tag="JWH23_RS00260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="WP_277407533.1"
                     /transl_table=11
                     /translation="MAFDGIVMANLTAEMKDRLEGGKISKIAQPEKDELLFTVKNQKNT
                     WKLLISASASLPLVYFTENNKQSPLTAPNFCMLLRKHIGNGRIMRISQPGLERILCMEI
                     EHLDELGDRRTKKLMIEIMGKHSNIIFCNEDDMILDSIKHISAQVSSIREVLPGRKYFI
                     PQTMDKKDPLTITEEEFISTFRHAAAPVQKSLYLNLTGISPIIGHELCHLASIDGDHSA
                     NELSETELLHLYRMFSFMIEDIKKGSFKPNIVYKNEEPVEFAAVSLTCYEGSDYEAKTF
                     ESISTLLETYYASKNAVTRIRQKSVDLRKIVQTALERNYKKYDLQEKQLKDTEKKDKYK
                     VYGELLNTYGYELTGGEKKLTCLNYYTNEEITIPLDDQLSARENAQKYFDKYNKLKRTF
                     EAVTGQISETRQEIDHLESISAALDIALLEEDLVQIKEELMEYGYVKHRRSGDKKPKIT
                     SKPFHYISSDGFHMYVGKNNYQNEELTFKVASGNDWWFHAKGIPGSHVIVKSEGKELPD
                     RTFEEAGALAAYYSKGRENDKVEIDYIQKKQIKKVAGAAPGFVIYHTNYSMVAEPKIDS
                     EAVNS"
     gene            13082..13960
                     /locus_tag="JWH23_RS00265"
     CDS             13082..13960
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545127.1"
                     /locus_tag="JWH23_RS00265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="WP_277407534.1"
                     /transl_table=11
                     /translation="MIKSMTGFGRYETVTDEYKISVEMKAVNHRYLDLSIKMPKKFNFF
                     EAGIRNLLKNYIQRGKVDVFISYEDYTENKLCLKYNSALAAEYMGYFAKMEEQFGIQND
                     IKVSALARCPEVLIMEEVPEDEDQMWKLLSETVEEAAKRFVETRLTEGENLKTDLLGKL
                     NLMNDILEFIEERSPKILVEYRAKLEDKVKELLAGAAIDEGRIASEVTIYADKICVDEE
                     TVRLRSHIEHTRSELEAGGIVGRKLDFIAQEMNREANTILSKASDLEVSDKAIILKTEI
                     EKVREQIQNIE"
     gene            13973..14593
                     /gene="gmk"
                     /locus_tag="JWH23_RS00270"
     CDS             13973..14593
                     /EC_number="2.7.4.8"
                     /GO_function="GO:0004385 - guanylate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="gmk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775024.1"
                     /locus_tag="JWH23_RS00270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="guanylate kinase"
                     /protein_id="WP_277407535.1"
                     /transl_table=11
                     /translation="MNEHGMLVVVSGFSGAGKGTLMKELLNRYENYALSISATTRAPRT
                     GEVDGKEYFFVTEEHFNDMIEKEELIEYAQYVNHSYGTPKEYVLNQMKKGKDVILEIEI
                     QGALKVKERFPEAILLFVMPPNAQELKRRLIGRGTESMEVINARLRRASEEAQGMEAYD
                     YILINDEIDTCVEEMHLMIQVQHKRASNNMAFLSQIREELKNI"
     gene            14620..14874
                     /gene="rpoZ"
                     /locus_tag="JWH23_RS00275"
     CDS             14620..14874
                     /EC_number="2.7.7.6"
                     /GO_component="GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA]"
                     /GO_function="GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rpoZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780437.1"
                     /locus_tag="JWH23_RS00275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="WP_104436169.1"
                     /transl_table=11
                     /translation="MLHPSYSDLINVVNSEVEPGDAPVVQSRYSIVIAAAKRARQIIGG
                     SDTEIPGAGKKPLSVAVEELYEGKVKILSEADATEEDDN"
     gene            14944..16284
                     /gene="rimO"
                     /locus_tag="JWH23_RS00280"
                     /pseudo=""
     CDS             14944..16284
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0008172 - S-methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /gene="rimO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604173.1"
                     /locus_tag="JWH23_RS00280"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKLLCISLGCDKNLVDTEMMLGLLNRDGYTFTDDEYEADVIVINT
                     CCFIGDAKEESVNTILEMAQRK"
     gene            16268..16810
                     /gene="pgsA"
                     /locus_tag="JWH23_RS00285"
     CDS             16268..16810
                     /EC_number="2.7.8.5"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507219.1"
                     /locus_tag="JWH23_RS00285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="WP_277407536.1"
                     /transl_table=11
                     /translation="MNLPNKLTVLRVIMIPFFVLFLLLDGGNNVTYRYIAAAVFIVASF
                     TDLLDGKIARKYNLVTNFGKFMDPLADKLLVCAGLVCFTALGQLPAWIVIVIISREFII
                     SGFRLVASDNGVVIAAGYWGKFKTASQMVMSVLLILNIPALSIVTNAFIWIAAILTVVS
                     LVDYIAKNYRVLTEGSM"
     gene            16814..18058
                     /locus_tag="JWH23_RS00290"
     CDS             16814..18058
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604171.1"
                     /locus_tag="JWH23_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence/damage-inducible protein A"
                     /protein_id="WP_170823594.1"
                     /transl_table=11
                     /translation="MVVELISVGTEILLGNIVNTNTQYLAEKCALLGLSMYHQVTVGDN
                     RERLFAVLETALNRSDVVILTGGLGPTEDDLTKEVCAEVMGFDLVEDEHTRERISKYFK
                     NNIYKEIPDNNWKQAMIPQGATVLDNSNGTAPGLIMEKDGKTAILLPGPPNELKPLFTE
                     QVFPYLQSRQPEVIRSQMVKICGYGESQVEDKLIDLINTQTNPTIATYAKTAEVHLRVT
                     AKAANEEEAKTLLKPVVKEIKNRFDNAVYTTKEEETLEMAVVRLLKKHELTVTTAESCT
                     GGLIAGRLVNVPGASEVFREGFITYSNKAKRKYLDVSKGTLKKYGAVSEQTAKEMATGG
                     VFASDADACIAVTGIAGPDADGEKPVGLVYIACYMKDKVKVEEYHFKGNREKIREQSVV
                     KALDFLRRSILSNYH"
     misc_feature    complement(16837..16851)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 20.65"
                     /tool="antismash"
     misc_feature    complement(16837..16852)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.89"
                     /tool="antismash"
     gene            18081..18947
                     /locus_tag="JWH23_RS00295"
     CDS             18081..18947
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407537.1"
                     /transl_table=11
                     /translation="METRLMKKQMTAAYQYSYVRLVLNYYTLLETVAEEAPQTQQMTDY
                     LKRLNSLIGECISGKEVNKDLLALRKDMTREVEVLTSYADCFQIFEYILNRMERKFISS
                     GKTNDGDEAFLKRLMGFITDTSDSAVMNGRIKQIMGQLPVRLTKQKFYSLLMDGLSVYL
                     GSPRENLRDMMYTLRTESMVSLPEGMDAGHKDIYEILQQFKRMDYRDMTFESYEEASAK
                     LSYASQVLTDESGFYVMVQDVINDLCVLMFARQDAVIDVSEEEFYRSVISSIQVKLDQE
                     DFTLGRR"
     gene            18934..19413
                     /locus_tag="JWH23_RS00300"
     CDS             18934..19413
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JWH23_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_277407538.1"
                     /transl_table=11
                     /translation="MGEDEFLHQLTQLEGRQEVYYERYLKVEVLNEESADDGDYIRSMN
                     VDRLLSGSSFADLIIEEGRETLSGEENAIVDKSYLDQQAEAYIQELEELFAEAPKPVVR
                     AVMAKILSDLPVYFNSIDEIQEYVRGSLSSCLDRAEKETCMELLEELMDYENKLV"
     gene            19394..19714
                     /locus_tag="JWH23_RS00305"
     CDS             19394..19714
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780442.1"
                     /locus_tag="JWH23_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_104436174.1"
                     /transl_table=11
                     /translation="MKISWYNRLYVGEKAKKKRYRIIQGIRKGKPGLDIYVITPASNGN
                     NILDIFPAAEIISPFYKEKEFFILGIASDYWEAMEVAGRVIHDLYRKTEGFNLSDFLKS
                     DR"
ORIGIN
        1 tttgtatcgg ccggtagctc aggcgccata ctggttggcg gacaggtgat agtcggacgg
       61 atcaagggtg tagaaagacc accgcttgca ccccttattc caacagaaaa aggcgtttcc
      121 ctgctgatag actgcggcgc taatgtggat gccagaccct ctcatctggt acagtttgcc
      181 caaatgggct ccttatatat ggagcatgtt gtaggcatta agaatccaac agttgccatt
      241 gttaacatcg gcgcagagga agaaaaggga aatgcgctag tgaaagagac atttccgctg
      301 ttaaaggatt gtaaggatat tcattttacc ggaagcattg aggccagaga gattccgcat
      361 ggcggtgcag atgtcatagt atgtgaggca tttgtgggca atgtaatcct gaagttgtat
      421 gaaggagtgg gcgcgacctt gattaacaag gtaaagagtg gtatgatggg tagcttcagg
      481 agcaaaatcg gtgctctgct tgtaaaacct gctttgaaag agactttaaa atcatttgat
      541 tcttcccggt atggcggcgc tccgctttta gggctgaatg gtctggtggt aaagactcat
      601 ggtaactcca aagcagcaga aataaaaaac tccatacttc aatgcgtaac cttcaaagaa
      661 cagggaatta atgataagat aagagaaagt cttaaaatga aagaagaaaa tcaagaatag
      721 gagagaggaa ggaaaacaaa tggaattcga aaaattacag aaaattattg cagaggtatt
      781 aaatattgag ccggaggaag ttactatgaa ctccacattc gtggatgacc tgggtgctga
      841 ctcccttgat gtgtttcaga tcgttatggg cattgaagag gaatttgata ttgagattcc
      901 aactgaagtg gcagagaaca ttgtaagtgt cagcgacgca gtagaacagg tcaagaatgc
      961 attaaattaa tagatgtggg tgctgctagg atcgtttggt ctgaccgcag caccttcttt
     1021 cataataggg ggtaatcggg tacccctcta tgtccaaaat acgtggacaa taataaggaa
     1081 caccctacgg gtacccctct atgtccaaaa tacgtggaca ataataagga aataggaaag
     1141 aggaatgaga tgagcagaag tatgaaagaa ctggaagaac ggatcggtta tcgtttttcg
     1201 gacaagcatc ttatgacaca ggccatgacc cacagctcct atgccaatga acaccgactg
     1261 aataaaacgg aatgtaatga gagacttgaa ttcttaggtg attcggttct ggaagtggtg
     1321 tccagtgatt gtctgtatca taaatatcca caaaagccag agggagattt aacaaaaaaa
     1381 ttcgtgccag cattgtttgc gaacctaccc ttgcttattg tgcagaagtg ataaagcttg
     1441 gggaatatct ccttttagga cggggagagg aagcaacagg ggaagaggga gagcttcaat
     1501 tgtttcggat gcaatggaag cactgatagg agccatttat ctggatggtg gttttgctaa
     1561 tgcaaaaaga gtttatcctt cgtttcatca tgaatgatct ggaacataag cagctctttt
     1621 ttttgatagc aagactatct tgcaggagat tgtacaaagc cagacggaag aggcgttaac
     1681 gtatgaactc ttaaaagagg aaggcccgga tcataataag atatttgaga cgcaggtaaa
     1741 aatcggggat gcgattatcg gacagggaac tggaagaaca aagaaagctg cggagcaggt
     1801 tgcagcttat cacggaatat taaagctgcg tggacaataa taaggtaaca cccttcgggt
     1861 atccctttat gtccaaaagc gtggacaata ataagataac acccttcggg tatcccttta
     1921 tgtccaaaga acgtggacaa taatatggaa aagaaagggc agctggtgat ggctgtcagc
     1981 gtggagtatg tatttaaaaa agtatagaga ttcaggggtt taaatctttt gccaacaaaa
     2041 tagtattcga gttccacaat ggaattacag gcattgtagg ccccaacgga agcggtaaga
     2101 gcaatgttgc tgatgcggta cgctgggtac tgggtgaaca aaagtaaagc agttaagaag
     2161 ttccagtatg caggatgtta ttttttttcg ggaacggaaa tgagaaaacc ccagggattt
     2221 gcctatgtgg ccataacgct tgacaattcg gatcaccacc tatccattga ctatgatcag
     2281 gtgacagtat caagaagagt ctaccggtct ggcgagagcg aatatatgat caatggaagt
     2341 gcctgccggt taaaagatat taatgagctt ttttatgata ccggtattgg taaggaaggt
     2401 tattccatta tcggacaggg tcagattgat aaaatcctaa gcggaaaacc ggaagaacgc
     2461 agagagcttt ttgatgaagc tgcgggaatt gttaaattta aacgccgtaa agccattgcc
     2521 cagaaaaaac tggaggatga aaaacagaat ctggttcgtg tcagtgatat tctttcggaa
     2581 ctggaaaaac aggttggtcc tttggcaaaa cagtctgagg cagcaaaaga atatttaaga
     2641 ttaaaggaag aactgaaaaa gtatgatgta aatcagttcc tgatggaaac agaaggactg
     2701 cgctttcaga tgaaggaagt ggatgagaaa gaagtaattg tcagccgtga tctggaagaa
     2761 gctaaaaacg catcagaggg catcagagag cagtatgaga cccttgatac atatttatcc
     2821 cagttggatg aatctatttt agtaagccgc aatgaaaaaa acaactctga tatagagact
     2881 ggaaacttgc agggacgcat caatgttcta aaagagcaga tcaatacaga acagatgaat
     2941 gcggagcaca ttgcaggcag ggtgaggtcc attcactccg agatagatat gaaaaaatcc
     3001 caggcgtcct cctatgaaga ggaacgttct gtcattgcag aatcagtaaa gactgctatg
     3061 gagcagttaa agcagggaga aacggtactt tcagcagaag acgaaaaaat tcatcttctg
     3121 gaacaaagga ttgaatctgg taaggggagc attattgagc ttctcaatga aaaaagccac
     3181 cttaaatgcc aaacagcagc gttatgagac catgcttgag caggtaaatg tacgaagatc
     3241 tgaagtactc caaaaactcc tcaaatataa aagtgatgaa tcagaacagg atcaacagct
     3301 actggttctt caaaaagaag cggatgaaat tgatgctggt atttttaagt acaatgaact
     3361 tcaggaacaa agcgaaagca gagccgggga actggagcat gaagttaaac gtcttgggaa
     3421 gaatttaaac gacagacagc aggagtacca caccaattct accaagctgg aatctcttcg
     3481 aaatcttgcc gaacggtatg aaggatatgg aggcagcata cgccgggtca tggaagtaaa
     3541 ggaccgggtg catggaattc acggtgttgt ggcggattta attactgttt ctaaaaagta
     3601 tgaagtcgcc attgaaacag cgctgggtgg aagtatccaa aacgttgtta ccgactcaga
     3661 ccagacagca aagcagctga ttgagtattt aaagaaaaac aaatacggaa gagctacatt
     3721 tctccctctt accagcattg gtaataaaaa cggtttcaat caggaaaaag cccttagcga
     3781 accaggagtt ttaggacttg caaattctct ggttgaagcg gattctcagt accagggact
     3841 tttaaactat cttcttggaa gagtggttgt tgtagatacc attgaccatg caatcgcact
     3901 tgctaagaaa tttcagtatt ctttaagaat tgttacactg gaaggagaat tgttaagtgc
     3961 cggaggttcc atgactggtg gtgctttcaa aaacaccagc aatttactgg gacgaaagcg
     4021 ggaaatggag gaactggaag cagcctgtga aaaggcgctg aaagaaaccg ggcagattga
     4081 gacagagctg gtcatgaacg aagggctttt aagtgaatgt aaagaagagc ttgaaaccat
     4141 ccgaagtgag aagcagaagc tttatttaag acagaatacg gttaaaataa atctgcgcag
     4201 gattgaagat aaaaaagcag agattataga atcctacggt gatttagaac tggaaaataa
     4261 ccagctcgag gtacagaaaa aagagattgg cgaaaacagg caggaattaa tccttgcagt
     4321 ggaaaagctt gcagttcgga gccaggatat agacggtgac ttagagagtc tgaatgctga
     4381 tcttgaaaaa gcaaaagccg acagagaaaa attctccaga gaattgtcag aagcccagct
     4441 ggcagcttcc agtctgaagc aaaaagatga ttttgagctg gaaaatatcc gacgggtaaa
     4501 agatgagatt agtagattag aagaggaact gtttaagctg tctcagggat ccagcggatc
     4561 tggcctgatt attgaagaaa aacaaaagga aatagaatct attaaagcca gaattgcaga
     4621 gatcagttca gccagagaaa cactggatga ggcaattaat gagaaactgg ctaaaaaaga
     4681 gatgaaatca aaagagcaga aagaactgtt ccaaaaacga gaagaattca cccagcgtat
     4741 cagtctgctg gataaggaac tgttccgcct tcagagccag aaagagaaaa tggaagagcg
     4801 tatggataac cacgtaaact atatgtggaa tgagtacgaa ctgacatttt caaaagcgga
     4861 agctttaaga aatgaagaat ggacctctct gccggacatt aagaagatga tcggttcctt
     4921 aaaagaagag atacgaaagc taggcaatgt caatgtaaat gcgatcgaag attataaaga
     4981 agtttcagag cggtatgaat ttatgaagac acagcatgac gatctgatta ctgccgaggg
     5041 aaccttatta aaaattatcg atgagcttga tactggaatg agaaaacagt ttgaagaaaa
     5101 attccgggaa atcagagtgg agtttgataa agttttcaag gaactgttcg gtggcgggcg
     5161 tggtgcactt gaacttgtgg aagatgagga tattctggag gcgggaatac agattgtttc
     5221 ccagccgcct ggcaaaaagc ttcagaatat gatgcagctc tccggtgggg aaaaggcact
     5281 gacggccata gcactgcttt ttgcaattca gaatttaaag ccatctccgt tttgtcttct
     5341 tgatgagatt gaggccgcgc tggatgactc caatgtggat cgttttgcaa aatatttgca
     5401 taagttaaca aaatttacac agtttattgt tattacacac aggcgcggta ccatggtgtc
     5461 tgctgatcgt ctgtatggta tcaccatgca ggaaaaaggc gtctcgacgc ttgtatctgt
     5521 taatctgatt gaagaagatt tagaaagtta aggaggccga caatgggaga aaaaaaggga
     5581 ttctttggaa aactggtagc tggactgcaa aagacaagag ataatattat cgcaggtgtg
     5641 gattccattt tcaacggttt ttcggccatt gatgatgagt tttatgagga aattgaagaa
     5701 attctgatta tgggtgatct gggtatccag acgactacat caattgtgga agacttgaga
     5761 aaaaagtaaa agaaaaaaac atcaaggatc cgtcagaatg taaacagctt cttatggaca
     5821 gtatcatgga acagatggat ttgggagaaa atgcatatga atttgagagt cgtcgctccg
     5881 ttttattaat aatcggagtg aacggagtgg gtaaaaccac ttctgtaggt aagctggcag
     5941 gacaaatgaa agacgatgga aaaaaagtga tcgttgcagc tgccgacaca ttccgcgcag
     6001 ctgcaattga acagctgaca gaatgggcga gaagggcaaa cgtggatatt attgctcagc
     6061 aggaaggctc tgacccggcg gcagttgtat atgatgcagt tgctgcagca aaatccagac
     6121 aggcggatat attaatctgt gatacggcag gacgtctcca caataaaaag aacctgatgg
     6181 aagagctgaa aaaaattaac cggataattg acaaagaata tccagaagca tatcgggaaa
     6241 ctcttgtggt cttagatgga actaccggcc agaatgctct tgtgcaggcg aaacagttca
     6301 tggaagtggc tgatattacc ggaattattc ttactaagct ggatggtacg gcaaaaggag
     6361 ggattgcggt agcaattcag tccgagctgg gaattccggt gaaatatatt ggtgtcgggg
     6421 agaagattga tgacttgcag aaattcaatg caaaagaatt tgtaaatgca ttgtttcacg
     6481 tagaagagaa agaagaggcg taaattatgt tgacattgga aaagtttgag gaagcatcag
     6541 aagtcgttgg caaggttacc ctggaaacaa agcttatata cagtgagtat ttttcagctc
     6601 agactggtaa taaagtgtat tttaagccgg aaaatatgca atatacaggg gcctataaag
     6661 taagaggtgc ttattataaa atcagcactc tgacagagga agaaaaatcc aaaggtttga
     6721 ttactgcctc tgccggcaat catgcacagg gtgttgccta tgctgcaaag cttgccggga
     6781 ttcccgctac cattgtcatg cctacaacaa ctcctctgat gaaggttaac cggacgaaag
     6841 gatacggagc agaagtgatt ctggaagggg atgtctttga tgaagcctgc aatcatgcct
     6901 ataagctggc agaagaaaga ggacttacct ttgtacatcc gtttaatgat ttagatgtgg
     6961 caaccggaca gggtaccata gcaatggaaa tcatcaaaga acttcctaca gttgattata
     7021 ttctggttcc aatcggagga ggcggtcttt gcacaggcgt ttccgtattg gcaaagatgt
     7081 taaatcccaa aattaaggta attggtgtgg agcctgcagg tgcaaactgc atgcaggagt
     7141 caattcgcca ggggaaagta gtatctcttc caactgtcaa tacaatcgca gacggtacag
     7201 ctgttaaatg tcctggagac aaactttttc cctacatcca ggaaaatgtg gacagtatta
     7261 taacaattga agattcagaa ctgatcgtag catttttaga tatgatggaa aatcataaga
     7321 tggtggttga aaactccgga cttcttacgg tcgccgcttt aaagcatatt gatgtggata
     7381 ataaaaagat tgtatctatc ttaagcggcg gaaatatgga tgtcattaca atggcctcta
     7441 tcgtacagct gggactgatt cagagagacc gtatcttcac cgtctccatt ctgcttcccg
     7501 ataagccagg agagctggct aaagtatctg cccttcttgc aaaagagcag ggcaatgtca
     7561 tccgtctgga acataaccag tttatcagca ttaaccggaa tacagcggta gaattaagaa
     7621 ttacactgga agcttttggt acagatcata agaatgctat aatggcagct cttacttctc
     7681 aggggtatcg gccaaagctg gtgaaatcta aaggaacata tggggactaa tgaaagaaac
     7741 aaaaagaagc agaaactgga cgtactccgg ttctgcttct tttattatag acattttagt
     7801 gatttatgta ccttggacag cagtcgtaca gatactgaat aagattcttt attattcata
     7861 tacaaaagtg catcttctct tgaatatttt atcacatgtt ttaaattaac tattgtggat
     7921 cggttgcatt gacaaaaatg tgagttcaaa gaagcactca gttctttgag ggaaccagac
     7981 atttggcgga aaccattcaa agtatgaata attattttgt gatactcttc acttgtagta
     8041 atggcaacaa tatcatcctg atccatgacc aatgatgctt tatcaacctt tatggacaaa
     8101 agacggtcag atgaatctaa ttgattccga tattttgttt ccgcattctt tatgcaggaa
     8161 tagatctgtt caggaagatc gcttacttta tcttttacaa taaagtccat ggcttctact
     8221 ttgtattgaa aggtcagata tgccatttca ctatgtgttg ttacaaagac aatatatcca
     8281 cgtggatcat atttgcggat ttcttctgcc aattggattc cattcttatt cgctcttaaa
     8341 tctatatcca gaaaatacag gccggtaata ttagagtctt ttcttacctt taataaatca
     8401 tccggataag atgtagcgca atatagcaat gactccgttg agttcattaa taaatatttt
     8461 tctataatag atgtccagcg taataattgt cttttatcgt cttcgcaaag atatatatga
     8521 atcataattg ctccttgtta ccataatgca tcagaatcag ttcctgagta aaatatggtt
     8581 ctttataaat tgtgttccat accacattat cataatgatt cagtaccatg gaaacattat
     8641 acaaaccgac gcctctgttt tcaccttttg ttgatacccc ctgttcgcgg agcttatata
     8701 aagaatgatc aggaggaaga gcagtatttt taataacaat ataaagatcg tcttctttat
     8761 aaaacataca aaaatgcaac tctttttcgt ttgtttctgc agttgcctca atggcattgt
     8821 ccaaaaaaat gccaataatc cgggataaat caagagcatc catatacaaa ttagtaattg
     8881 gttctgttaa ttcaatttct gctttaattc ctgtttccat ggaataaata aatttagagg
     8941 aaaggaggct ttttaatgct gtgtccttta tatttgataa agtgccaagt tttgtatctg
     9001 attccgtgaa agaatgactg attggtatta ttttttctcc gaaatatttt ttcaatccct
     9061 gcaaatcatt atcttccata aatccggaca ttgttataag aatattcata taatcatgtt
     9121 tgaactttcg cattgtgcca taggacattt caagcctgtc tgtataagtt ctaagatttt
     9181 caaattgcga catacggttt ttataggaaa gctcccccat agttgcttta tagatggaat
     9241 acattaaaaa aacagtgatt gcaaacagca ataaaaatag tattccattc aaagcaatta
     9301 caccataatt gtatcctaat tgttcaccgt atgaaaaatt aaaaatataa aagaacacta
     9361 aaataaaaag gtcagtaaag atggccttta aaagatgacg attggttaaa tactttgaca
     9421 aattaagctt tttatgaaaa taccaaccca gcattttagt agttactccg caaaatacac
     9481 agtaaaatat tgaaaaaata attgtcagat tgtcccgtac taataattct gccatatcca
     9541 tacgaagaaa ataacttgtc agccacatga taatataatt aaaagtgact gagtataagt
     9601 aaccaaaaag aaagcatgag gaagagaaaa agtcaaattt agaaaagacc atggctataa
     9661 tcagtattcc tccataagtt aaaaaagtgc ttatttgacc aaacattcga ttgaacaggt
     9721 atactgtcag gaatgagtat aaaaataaaa tcagtttctt aaaaatgtta agtttatgag
     9781 gaagtaaatg aacgcatgaa aaataactgc ataggcttcc tgttaatgaa taaattaagt
     9841 aagtcatatt atgccacctc atctgccggg tactattatt ctacaattca tatgacaaaa
     9901 agtaaagtga aattgtattc aaaagtcaag aaattgacgc ttaggaataa aaaaacaacg
     9961 ttaattgcaa aaacatttgt aagttagatt ttatggtaaa atggcattgt ttggtggtga
    10021 taaagtgatt atttcgagat tttcagctgc tctctgttgc ttattaaata aggttaatcc
    10081 cagagatgaa gatgataata ttacaataca atatggagtt gagctgatat tagataattt
    10141 attaaagctt ttgttcattc agcttctagg ttttttattc ggtatgggag ttgaaacatt
    10201 cataatttta tttgtatttt gttctttgcg tctgcaggct gggggaattc acgcaaaaac
    10261 aaatgcaggt tgtttttttg gtatggtgac agtttgggtt ctgtctttat caggaacaat
    10321 tgttttcaaa ttaagcatat ttaatgtact ggttatctat gtgtttagcc tgattatttt
    10381 tatatgtctg gtaccgaaaa gcaaaaatat tacttatttt actcgctcag ctatattaaa
    10441 gaaaaaaaat tatatcaatt ttttttgtta accctttagt attgattgct atttacaatg
    10501 aaaatctgag agagttgatc atttatccgg ttgctttgga agtactaaca ttacttcctc
    10561 caaataaaga tgttatgaaa ggagatttaa aaaatgaaga aagaaattgt taaaggatta
    10621 caaaaggttt tgcttaaggt ttctgttaat tctgttgaaa aaagcattcc agcaactatg
    10681 tatgaaagag aaattccaaa agctgttctg aaaaaacatg agactgaggc aaagaataac
    10741 taaatttaaa tgtttctttc gtagtactat ttattagcct aatatttata atctcaatca
    10801 tgtgatttta ttgtcattag gcaggggagt atgaccgtaa ttatatcata tgaattggtc
    10861 atctatgagt gttaatatat tgaaatttat atatctaaat gttttactgt gcgtatgatt
    10921 aattgggaga aagtacagat gaaaacaaaa atgtttcgaa aatttcttgg attggaacgt
    10981 gaaattcctc attactctgt tcttaatgat gatacatcaa gtgacagtaa gagaatgatc
    11041 attccaaggg aatatctaat agaagtaata acaacaattc ctgaacggcg ggttgttatt
    11101 gaacatttag atgtttatca agaataatac atcggaaagt actaaaaagc agataagaag
    11161 agagttaagc tttcttctta tctgcttttt aaggtttaac tattcactgc ttccgaatct
    11221 atctttggtt ctgccaccat agaataattt gtatgataga ttacaaaacc gggagcggct
    11281 cccgcaactt ttttaatctg ttttttctgt atataatcta tctcaacctt atcgttttct
    11341 cttcctttag aatagtaagc agccagagca ccagcctctt caaaggtgcg gtcgggaagt
    11401 tcttttcctt ctgatttgac aatgacgtgg gagccgggaa ttcctttggc atgaaaccac
    11461 cagtcattac cacttgcaac cttaaaagtt aactcctcat tctgatagtt gttctttcct
    11521 acatacatat ggaagccgtc agatgaaata taatgaaaag gtttgctggt tattttcggt
    11581 tttttgtctc cgcttctgcg atgcttgaca tagccatact ccataagttc ttctttaatc
    11641 tgcacaagat cttcttccag caaagcaata tccagtgctg cactgatgga ctccaggtgg
    11701 tcaatttcct gccgggtttc actgatctgc cctgtaacgg cttcaaacgt tcgttttaat
    11761 ttattgtatt tgtcaaaata tttctgagcg ttttccctgg cagaaagctg gtcgtctaag
    11821 ggaatggtaa tctcctcatt ggtataatag ttaaggcagg tgagcttctt ttcacctcct
    11881 gtcagttcat aaccgtaggt atttaaaagc tctccatata ctttatactt atcctttttc
    11941 tccgtatctt ttaactgctt ttcctggaga tcgtattttt tgtaattacg ttccagagcc
    12001 gtctgtacaa tcttccttaa atcaacggat ttctgacgaa ttcttgttac tgcatttttg
    12061 gaagcatagt aggtctctaa aagagtactg atggattcga aggttttggc ttcgtaatcg
    12121 cttccttcat agcaggtgag tgaaacagct gcaaattcta ccggttcctc attcttataa
    12181 acaatatttg gtttaaatga tccttttttg atatcttcta tcataaaaga gaacatgcgg
    12241 taaagatgta agagttctgt ctctgacagc tcattggcag agtgatctcc atcaatagaa
    12301 gccagatgac aaagttcatg gccaatgatg ggactgatac cggtcagatt taaataaaga
    12361 ctcttttgta ctggtgcggc cgcatgcctg aaagtagaaa taaattcttc ttcggtaatg
    12421 gtcaggggat ctttcttgtc catggtctgg ggaataaaat attttctccc gggcaatacc
    12481 tcacgaatgg aacttacctg ggcagagatg tgtttaatac tgtccagaat catatcatct
    12541 tcattgcaga agatgatgtt actgtgtttt cccataattt ctatcatgag ttttttcgtc
    12601 cgtctgtcgc ccagttcatc taaatgttca atttccatgc aaaggattcg ttccagacca
    12661 ggctggctga ttctcatgat tctgccattg ccaatatgtt ttcttagaag catacagaaa
    12721 ttaggagcag tcagaggact ttgcttgttg ttttccgtga aataaaccag tggtaagctg
    12781 gcactggctg aaattaaaag tttccatgta tttttttgat ttttaacagt aaacagcagt
    12841 tcatcttttt ccggctgggc gattttagag atctttccgc cttccagccg gtctttcatt
    12901 tctgccgtga gatttgccat aacaattcca tcaaatgcca ttgtttgtgt cctccggtaa
    12961 ggtttataga tggagatagt ataccacaga aacacggtta tgtaaaattt aaaaccattt
    13021 gacttgtttt ttattatgac ttataatggt ttatatctat ggcgaaagag aggcatttgt
    13081 aatgattaag agcatgacag gtttcggcag gtacgaaacc gtcacggatg aatataaaat
    13141 ttccgtcgag atgaaggctg taaatcacag atatttggat ttaagcataa agatgccgaa
    13201 aaagtttaat ttctttgaag cagggatcag aaatctgtta aagaattata ttcagcgcgg
    13261 taaagtagac gtttttatca gttatgaaga ttatacggaa aacaagctgt gtttaaagta
    13321 taacagtgcg ctggctgctg aatacatggg atattttgca aagatggaag agcagttcgg
    13381 aattcagaac gatatcaagg tttctgcact cgccagatgt ccggaagtac ttattatgga
    13441 agaagtaccg gaagatgagg atcagatgtg gaagcttctt tcagaaacag tagaagaagc
    13501 tgcaaagcgt tttgtggaaa caagattaac agaaggcgaa aacttaaaga cggatcttct
    13561 tggtaagctg aacctgatga atgatattct tgaatttatt gaagagagat ctccaaagat
    13621 tcttgtggaa tacagggcaa agctggaaga taaggtgaaa gagcttctgg caggagctgc
    13681 cattgatgag ggcagaattg cttcggaagt aaccatttat gcagataaga tctgtgtgga
    13741 tgaagagacg gttcgtttaa gaagtcacat tgaacataca cggtcagaac tggaagctgg
    13801 aggaattgtg ggcagaaaac tggatttcat cgcccaggag atgaatcggg aagccaatac
    13861 cattctttca aaggccagtg atttagaggt ttcagataag gcaattatat taaagactga
    13921 aattgagaaa gtacgggaac agatccagaa tattgagtag gagaatcgca gtatgaacga
    13981 acatggcatg ctggtagttg tatccggttt ttctggtgcc ggaaagggca cacttatgaa
    14041 agagcttctg aaccggtatg agaattatgc gttgtctatt tccgcaacca caagagcgcc
    14101 cagaacgggt gaagtggatg gaaaagaata tttttttgtt acagaagaac attttaacga
    14161 tatgattgaa aaggaagaat tgattgaata tgcacagtac gtcaatcatt cttatggcac
    14221 tcccaaggaa tatgttttaa accagatgaa aaaggggaaa gatgttattc ttgaaataga
    14281 aattcaggga gcgctaaaag taaaggaacg gtttccggaa gcaatcctgc tgttcgtcat
    14341 gcctcctaat gcacaagagc tgaaacgccg tctgatcggc agaggtacag aaagtatgga
    14401 ggtaattaat gccagacttc gcagggcttc agaagaagca cagggcatgg aggcttatga
    14461 ttatatttta attaatgatg agatcgacac ctgcgtggag gagatgcatc ttatgatcca
    14521 ggtacagcat aagcgcgctt ccaataatat ggcattttta tcccagatcc gggaagaatt
    14581 aaagaatata taaactggtt tgagaaaggg gaatggaaga tgttacatcc atcttattca
    14641 gatttgatta atgtagtaaa cagtgaggtt gagccgggag atgctccggt tgtacagagc
    14701 cgttattcta tcgtgattgc cgcagcaaag agagcaagac agatcatcgg aggctctgac
    14761 actgagattc cgggagccgg caagaagccg ctttcagttg ccgtagaaga gttatatgag
    14821 ggcaaagtga agattttaag cgaagcggac gcaacagaag aagacgataa ttaagaagag
    14881 ggccgtctcc gttatggtag atggcctttt ctttcaggaa aaacgaatga caggagttta
    14941 aacatgaagt tattatgcat atcactgggc tgtgataaga atctggtaga tacggagatg
    15001 atgttaggcc ttttaaatag agacggttat acttttacgg atgacgaata tgaggcagat
    15061 gtcattgtta tcaatacctg ctgttttatt ggcgatgcca aggaagaaag tgtcaatacc
    15121 attttagaga tggcacagag aaaatagacg gcaactgtaa ggcactgatt gttacaggct
    15181 gcctggcaca gcgttataag caggagatta ttgatgagat accggaagta gatggtattc
    15241 tgggaacatc aacctatgac gagatatcca atgttttaaa gaaggcattg ggaggcgagg
    15301 gatccgtatc ctgtttccat gaactggatt tacttcccca gacagaaaca gacagaatcc
    15361 ttaccacggg aggccattat gcctttttaa aaattgcaga aggatgtgac aagcattgta
    15421 cttactgtat catcccgact ttaagaggca attatcgcag tgttcccatg gaaaatctga
    15481 ttaaagaagc agagaaactg gctgataagg gcgtaaagga actgatttta gtagcccagg
    15541 agaccacttt atacggtgtg gacttatatg gaaaaaaatc tcttcctgaa ttattaaaga
    15601 aactttgcca gatatcaggg attcaatgga ttcgcatcca gtactgttat ccagaggaga
    15661 ttacagacga gctgattgaa gttatgaaag aagaaagcaa aatctgccgt tacttagata
    15721 tgccgattca gcatgcaagc gaccggatct taaaacgcat gggaagaaga acatcaaaag
    15781 agcagctaaa ggacatcatt aacaagcttc gcagtgaaat tcctgatatt gcactgagga
    15841 ctacactgat ctcccggatt tccgggagaa acagaggaag atcacgaaga actgatggaa
    15901 ttcgtggatg agatggagtt tgaacgtctt ggcgtattcg cctattccgc agaagaagat
    15961 acaccagcag cagaatttcc tgatcaggtt ccacaggaga ttaaagagga tcgcagagat
    16021 gcgattatgg agcttcagca ggagatttcc cttgaccttt cccagtccat ggttggaaaa
    16081 accctggaag taatgattga gggaaaagtt gctgacgaga atgcatatgt agcaagaacc
    16141 tatatggatg gcccgggagt ggatggtatg atttttgttc agacaggtga ggaactgatg
    16201 tctggggatt ttgcaaaaat ccgtgttacc ggagcgatgg agtatgactt aataggagag
    16261 ttggaagatg aatttaccga ataaactgac tgttttaagg gttatcatga ttccgttttt
    16321 tgtacttttt ttactgcttg acggcggtaa caacgtgact taccgctata tagctgcggc
    16381 tgtatttatt gttgcaagct ttaccgattt gctggatggc aagattgcaa gaaagtataa
    16441 tctggtaact aattttggaa aatttatgga tcctcttgcg gataaacttc tggtatgtgc
    16501 aggtttggtt tgcttcacag cacttggaca gcttccggcc tggatcgtga ttgtaattat
    16561 cagccgtgag tttattatca gcggattccg cctggttgct tccgataacg gagttgtgat
    16621 tgcggcaggc tattggggga aatttaagac agcttcccag atggtgatgt cggttctgct
    16681 gattttaaac atacctgcat tatcgattgt taccaatgct tttatctgga ttgcggcaat
    16741 actcacagtg gtttcacttg ttgattatat agcaaaaaat tatcgcgtac ttacagaagg
    16801 gagtatgtaa accatggtag ttgaactgat ttcagttgga acagaaattc ttcttggaaa
    16861 catcgttaat acaaacaccc agtatcttgc agaaaaatgt gcccttttgg gactctccat
    16921 gtaccatcag gtgacggttg gagataacag ggaacggctc tttgcagtac ttgagacagc
    16981 actgaatcgt tctgatgtgg taattcttac cggtggtctc ggaccaacag aagatgatct
    17041 gaccaaggaa gtatgcgcag aggtaatggg ctttgatctg gttgaagatg aacataccag
    17101 agagcggatc agtaaatatt ttaaaaacaa tatatataaa gaaatcccgg ataataactg
    17161 gaaacaggcg atgattcccc agggggcaac agtgcttgat aacagcaacg gaactgcccc
    17221 gggacttatt atggagaagg acgggaaaac agccatactg cttccaggac ctcctaatga
    17281 gttaaagccg ttatttaccg agcaggtatt cccttacctg caaagccgcc agccggaagt
    17341 aatccggtcc cagatggtta aaatatgcgg ctatggtgag agtcaggtgg aagataagct
    17401 gattgattta attaataccc agaccaatcc taccattgcc acttatgcca agacagcaga
    17461 ggttcattta agagtgactg ctaaggctgc caatgaggag gaagcaaaaa cccttcttaa
    17521 acctgttgta aaagagataa aaaaccggtt tgataatgct gtatacacaa ccaaggaaga
    17581 agaaaccctt gagatggcag ttgtgcggct tttaaagaag cacgaactga ctgttacaac
    17641 ggcggaatcc tgtacaggcg gtctgattgc tggcagactg gttaatgtcc caggtgcatc
    17701 agaggttttc agggaaggct ttattaccta ttccaacaaa gcaaaaagaa aatatttaga
    17761 tgtcagcaag ggcaccttaa aaaaatatgg ggcagtcagt gaacagacag ccaaagagat
    17821 ggcaaccggt ggtgtatttg cttccgatgc agatgcctgt atcgcagtga ctggtattgc
    17881 cggtccggac gcagatggag aaaagcctgt aggactggtc tatattgcct gctatatgaa
    17941 ggacaaagta aaggtggaag aataccattt taaaggtaac cgtgaaaaaa tcagggagca
    18001 gtctgtggta aaggctcttg atttcctgag gagatctatt ttatccaatt atcattaata
    18061 cccccgagag gagataacac gtggaaaccc gtttgatgaa aaagcagatg actgctgctt
    18121 atcagtattc ttatgtaagg cttgtattaa actactatac attactggag accgttgctg
    18181 aagaagcacc ccagacccag cagatgactg attacttaaa acggttaaac agcctgatag
    18241 gagaatgtat ttccggaaag gaagtaaaca aggatctgct ggcgctgagg aaggacatga
    18301 ctcgcgaagt agaggtactg acctcctatg ccgactgttt tcagattttt gagtatattt
    18361 taaatcgcat ggaacgaaaa tttatttctt ccggcaaaac aaatgacgga gacgaagcat
    18421 ttttaaaaag gctgatggga tttattactg acaccagtga ctccgctgtt atgaacggac
    18481 ggatcaaaca gattatggga cagctcccgg tccggctgac aaaacagaaa ttttacagtc
    18541 ttcttatgga tgggctttct gtttatcttg gctctccaag ggagaattta cgggatatga
    18601 tgtataccct aaggacggag tccatggttt ctcttccaga aggtatggat gctggtcata
    18661 aggacattta tgaaatttta cagcagttta agcgtatgga ttaccgggat atgacctttg
    18721 aaagctacga agaagcttct gcaaaattgt cctatgcaag tcaggtgctt acagatgagt
    18781 ctggttttta tgtgatggtt caggatgtga ttaatgattt atgtgtattg atgtttgcca
    18841 ggcaggatgc tgtcattgat gtcagtgaag aggaatttta ccgatctgta ataagcagta
    18901 ttcaggtaaa actggatcag gaagatttta cccttgggag aagatgaatt tctccaccag
    18961 cttacccagt tagaaggcag acaggaagtt tactacgaac gttatttaaa agtagaagtt
    19021 ttaaacgaag agtctgctga tgatggggac tatattcgtt ccatgaatgt agacaggctg
    19081 ttatctggaa gcagctttgc tgatcttata atagaagaag gcagagagac tctttctggg
    19141 gaagaaaacg caatcgttga taagtcttat ttagatcagc aggcagaagc ttatattcag
    19201 gagctggaag aactattcgc agaggcaccc aaaccggtgg ttcgagctgt catggccaag
    19261 atattatcgg atctgcctgt ttactttaat tcgattgatg aaatccagga atacgtaaga
    19321 ggcagtcttt caagctgctt agatcgcgct gaaaaagaaa catgcatgga attattggaa
    19381 gagctgatgg attatgaaaa taagctggta taaccgttta tacgtcggag agaaagcgaa
    19441 gaagaaacga taccggatca ttcagggaat caggaagggg aaacccggat tggatattta
    19501 tgtgattaca cctgcctcaa atgggaataa tatacttgat atctttccag ctgctgaaat
    19561 aatatctcca ttttataagg aaaaagagtt ttttatcctt ggcattgctt ctgattactg
    19621 ggaggcaatg gaagtggctg gacgtgtgat tcatgattta taccggaaaa cagaaggatt
    19681 taatctgtct gattttttga aaagtgaccg gtaagatgtg aggtgttcat gcttcatgta
    19741 attttattta ttttaaagct gttagggaat tctgatcctg atccttttag gacttattat
    19801 tctggcagtt cttctggtgc tgtttgtacc ggtccgttac cgtggagaag gatcttattt
    19861 tgaaaaagtg aagggaaata tgaaagtttc ctggctgcta catatccttt ctgtcacggt
    19921 tcggtatgaa gaagaagttg tcattgtgat ccgcttgttt ggatttcaaa ttatgagacc
    19981 aaagaaaatg gatgaagagc taaaagaggc agaagaaatc atggttcagg caatggaaat
    20041 aaaagagccg gagaccataa aagaagcacg gcaattaggt caggatgtga aaagaactgt
    20101 ccagggagaa cctctgtccc ggcagccagt acaaaaagag agcagacaga cagaacaaac
    20161 acaatcagag catatttcct ggcttcagag tttttatatt aagataaaga agaaaataat
    20221 ccatattctg gaaaaaatta agtacttata ccggaaattt tgtgatacac tgaagaacat
    20281 gaaagagaag aaggatgaaa tacaggcttt tctttccaac agcgaaaatc agaagaccgc
    20341 aaagctgctt tacagacagg tgaaacgtct gatccgtcat attttccctg ttaaggggca
    20401 tggtaacgtg acatttggtt ttgaagatcc ggcactgacg ggacaggtac ttatggcagc
    20461 cagtgtactg t
//
