LOCUS       NZ_CP070499            11236 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Natronosporangium hydrolyticum strain DSM 106523 chromosome,
            complete genome.
ACCESSION   NZ_CP070499
VERSION     NZ_CP070499.1
KEYWORDS    RefSeq.
SOURCE      Natronosporangium hydrolyticum
  ORGANISM  Natronosporangium hydrolyticum
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Natronosporangium.
REFERENCE   1  (bases 1 to 5699854)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Natrosporangium hydrolyticum gen. nov., sp. nov, a haloalkaliphilic
            actinobacterium from a soda solonchak soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699854)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) Bioinformatics, Leibniz Institute DSMZ,
            Inhoffenstr. 7B, Braunschweig 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-MAY-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: 999_Micromonospora_sp_DSM106523
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 580.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016925615.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 06:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,131
            Genes (coding)                    :: 5,046
            CDSs (with protein)               :: 5,046
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 18 of 85
            Pseudo Genes (incomplete)         :: 72 of 85
            Pseudo Genes (internal stop)      :: 4 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:11:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2844976
            Orig. end                         :: 2856212
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070499.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11236
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11236
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11236
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6236](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6236
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            76..237
                     /locus_tag="JQS43_RS12560"
                     /old_locus_tag="JQS43_12495"
     CDS             76..237
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JQS43_RS12560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JQS43_12495"
                     /product="hypothetical protein"
                     /protein_id="WP_239679254.1"
                     /transl_table=11
                     /translation="MTDSTKAPDPLFEEFDMEVYGRTSPNNMPFFASFVELTDDEQAEV
                     DEALALSE"
     gene            498..1655
                     /locus_tag="JQS43_RS12565"
                     /old_locus_tag="JQS43_12500"
     CDS             498..1655
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JQS43_RS12565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JQS43_12500"
                     /product="hypothetical protein"
                     /protein_id="WP_239679255.1"
                     /transl_table=11
                     /translation="MLGLGYGAGLRAVRASAPAADLFAVDRDPATVGLCRALYQVYFPS
                     IRFRMQVADAAQLMRDSVECFDLICVDLYDGASHPALVFDAGFWEDVRSRVADTGVVLV
                     NCWGLPEHLRPFAGASPQRALGEVLRTVWEEVRYLPARRNTTFVLTSTNVSLCIDEVTA
                     WPGLSAADRAVLATAACRLRHARTVPQGDAGGIAPARTQAAIDAEMARRWPAMLEVVGD
                     AAAAAGLPPAVASGRQVVLDPEIAVPVLRTLLDREAAEAAFIPTVAASLTFESDFRAGW
                     FGEWIADTHLDLAEHSPRWFYSTGLPQAMSMLANPYAPDWPWRDRLIEAAMRLSRAESV
                     SGTSLETTRQTSAMVVGGPPARTDAPESVPAAPYRRKELKDAEDN"
     gene            1639..2265
                     /locus_tag="JQS43_RS12570"
                     /old_locus_tag="JQS43_12505"
     CDS             1639..2265
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /locus_tag="JQS43_RS12570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12505"
                     /product="DUF6445 family protein"
                     /protein_id="WP_239679256.1"
                     /transl_table=11
                     /translation="MQKTIEVVDDFYVDVDRVRELAISMGDWANPKQEAGITYDFETYN
                     SFFNDQLVRVMRDLVGAEIHVDPKRMAFGVFGVFGADSHVDFTTHYDETDWSAIVYLVP
                     DDRCEGGLSFYRHKPSGLFGPPTEEQARQIGYDSRDAWLREHYYPDKMHPERWEETTHV
                     AMRYNRLILLRGSVLFHRASKGFGTTPDSGRLTQRFFFNDANRRD"
     gene            2266..2934
                     /locus_tag="JQS43_RS12575"
                     /old_locus_tag="JQS43_12510"
     CDS             2266..2934
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /locus_tag="JQS43_RS12575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12510"
                     /product="DUF6445 family protein"
                     /protein_id="WP_239679257.1"
                     /transl_table=11
                     /translation="MRKRLVVVDGFYADPDAVRQLALNSAFNSADRYNYPGWQSNKALR
                     TDALQARIEGILGRGIDVDPDRLTWGGFRLITDETGERTKVHADSAVDWAGMVYLTPDA
                     PSSAGTGFFRHRETGRERPPTDREARRLGFADADEFEERVVRRDMADLSKWEIVSWVGP
                     TYNRLVLFRGCEFYHAPLGGLGDRPANARLTHNFFFNERIEVGTVGRVLGKEVVSGGVR
                     "
     gene            2931..3590
                     /locus_tag="JQS43_RS12580"
                     /old_locus_tag="JQS43_12515"
     CDS             2931..3590
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /locus_tag="JQS43_RS12580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12515"
                     /product="DUF6445 family protein"
                     /protein_id="WP_239679258.1"
                     /transl_table=11
                     /translation="MRQAVTEVPHSLRGEVHIIDDFYEKPDAVRRLALDAEFVGFDGRA
                     NFPGGESVKAYWSPQHRDRFAELVGKAIDIDPSQWVFGKFRLALANETGRTKVHVDFVD
                     WTAVIYLSLDTDCSGGLGIYRHRETGLERVPRGSDLAAYRCSSLEEFDSRYIMRDSLHD
                     DRWELLHQVDIRYNRCVLFRGSRLFHGITRTFGGGSADGRLTQNFFFMDAVEGSSA"
     gene            3587..4273
                     /locus_tag="JQS43_RS12585"
                     /old_locus_tag="JQS43_12520"
     CDS             3587..4273
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1286: Pentapeptide
                     repeat protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /locus_tag="JQS43_RS12585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12520"
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_239679259.1"
                     /transl_table=11
                     /translation="MRSLLDMARAAVDLPGSPESYRLVTLDALGHTASGLELVSGTEVA
                     VADLRSVLGRSATELSRHRLSLQRLGVVAIEGEAARTVALLDGWRLIRSHGRQADLASA
                     DLRGADLTGADLIGSDLSYADLQGAWLDSANLRGARLRCTDLQGASLRCASMFGADLTE
                     ADLRRCDLRHSDLREATCAGVALRGADTWAAYVWEVDFSSAFLEGCEIGRADYRGADSK
                     PAGARS"
     gene            4318..5034
                     /locus_tag="JQS43_RS12590"
                     /old_locus_tag="JQS43_12525"
     CDS             4318..5034
                     /GO_function="GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /locus_tag="JQS43_RS12590"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on a
                     cobalamin-like analog.; Presence of a B(12)
                     (cobalamin)-binding domain implies dependence on cobalamin
                     itself, in one of its several forms, or in some unusual
                     lineages, dependence on a cobalamin-like analog.; Presence
                     of a B(12) (cobalamin)-binding domain implies dependence on
                     cobalamin itself, in one of its several forms, or in some
                     unusual lineages, dependence on a cobalamin-like analog.;
                     Presence of a B(12) (cobalamin)-binding domain implies
                     dependence on cobalamin itself, in one of its several
                     forms, or in some unusual lineages, dependence on a
                     cobalamin-like analog; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12525"
                     /product="cobalamin-dependent protein"
                     /protein_id="WP_239679260.1"
                     /transl_table=11
                     /translation="MIRAVQHATPPEAVADSGAGSELRVVLVCMPWALLDMPSLAVSTL
                     VPVATETAKTAQVDVVYANLRWCDYLTERTGGRIGAAQYQQVVEGYYHALGEWIFSPAL
                     YGADDPMDTPFYLTARANGADLDDACEMYRLAPGFVMALATELVDAGYDVVGLTSTFDQ
                     NMPSLALARAVKERSPRVITVMGGANCDDEQGAALHRSFPAVDFVVRGEGELTFLGYSG
                     PSASAMWSRRRPTRPR"
     gene            5001..6236
                     /locus_tag="JQS43_RS12595"
                     /old_locus_tag="JQS43_12530"
     CDS             5001..6236
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="JQS43_RS12595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12530"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_239674591.1"
                     /sec_met_domain="TIGR03975 (E-value: 3.4e-113, bitscore:
                     377.3, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4e-11, bitscore: 41.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEAETYQAALAGIDGLCWRATDGEPVTNPQQAPAVPVAAIPPAD
                     LTHFFEQFARATSVSDIDPKIQLEGARGCWWGEKHHCTFCGLNGSLMGFRAKSADRLLA
                     EITDAVRRHRTLDVVFADNIMDMGYIRDLVPKLSELDWDLRLFFEAKSNLSFAQLRALA
                     DGKVTQLQPGVESLSSNVLRLMRKGVAAWQNVRMLRDCRTLAIYPGWNILYGFPGETAD
                     DYAEVVRQMSFLRHLTPPEGCYRVVLTRFSPYFQDPSLGLINRGPSALLSKVYRLPKQE
                     LAQLGYLFESEKAGIRGEDVQALEAATRDWHTNHAGRRLVAIPDGSALRIVDERSPSPS
                     EHVLKDPVETTAFHATLRGRSAAAVHRQLEAAGLSVSGEQVADLIHSWHEQGWMFRDGD
                     YFVALPTGLSYD"
     misc_feature    5985..5987
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6284..6303
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.34"
                     /tool="antismash"
     misc_feature    complement(6764..6779)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            6810..7313
                     /locus_tag="JQS43_RS12600"
                     /old_locus_tag="JQS43_12535"
     CDS             6810..7313
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /locus_tag="JQS43_RS12600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12535"
                     /product="insulinase family protein"
                     /protein_id="WP_239674592.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 1.8e-18, bitscore:
                     65.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEVRFSVLSNGLRVVADRHPIVPLVAVNLWYAVGSRHERRGEHG
                     LAHLLEHLMCLGAARHSDYPRRLQAVGGGHFNATTNQDRTTYFQTAPPDFLPEVLRYEA
                     DRMASLGDSIDQERLDSQRRVVRNEARQSHDLRLAEALLSAAFPVGHPYHHPICQARVG
                     HGTG"
     misc_feature    6846..6848
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7362..8760
                     /locus_tag="JQS43_RS12605"
                     /old_locus_tag="JQS43_12545"
                     /pseudo=""
     CDS             7362..8760
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611928.1"
                     /locus_tag="JQS43_RS12605"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12545"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTMSNADLEPRAERPKRRVFTNEYKLAIVAEYDALTESGARGALL
                     RREGLYHSHLIEWRKARDAGALSGPTPRPGRKPQRSREQVELERLRRDNERMAAELART
                     KAALDVVGKAHALLGMLSESAEPTRENRSSR"
     gene            8814..9581
                     /locus_tag="JQS43_RS12610"
                     /old_locus_tag="JQS43_12550"
     CDS             8814..9581
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /locus_tag="JQS43_RS12610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12550"
                     /product="insulinase family protein"
                     /protein_id="WP_239674593.1"
                     /sec_met_domain="Peptidase_M16_C (E-value: 3.4e-18,
                     bitscore: 64.5, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLEQLTPGDVAGFHHRYYGPGNATLTLVGDIDPASAVRLAEAC
                     FGDLAPRIPPELPSMSPAGNRSQQAVVLESSNLPDDRVHLGFRIPPVGDPEHDVVTVLG
                     AVLGLGRGSLLARSLVEGGLVLPAGELMTVWRCSGDSSLLVAGLRAAPGVKAEEVEAAA
                     RDVMEAAWSQMVEDDLQRASALVVAQVLRHMETVSGRADFLSEMTTVFGDPLAATGFVH
                     RIRAVSLDDLRDTARRLLVSDDAFVVKCVARRD"
     misc_feature    9528..9530
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9600..10901
                     /locus_tag="JQS43_RS12615"
                     /old_locus_tag="JQS43_12555"
     CDS             9600..10901
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /locus_tag="JQS43_RS12615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS43_12555"
                     /product="insulinase family protein"
                     /protein_id="WP_239674594.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 2.8e-08, bitscore:
                     32.1, seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M16_C (E-value: 1.4e-14,
                     bitscore: 52.8, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAWPRCLWRDQTLMVNLPRRATAAATLVVESGAAADPPGRAGLA
                     TLLARLLQAGTPDMDAELAVGLERLGARFATHAGWDALRLTVEAPRSSLAAAVALMARH
                     LRTAPGASLSGTQLQQHASWVERRWARPAVVGEAAFREAALRGRCRMPLEGTPATARAL
                     ETVDVERAHGALSAVQRSLLMAGDLTSAEWDEVLGAAGDLGAPHETRQQHARRVEEPPF
                     GQTAPRLVVVDRPGSGSSLVSLGWRLPSYSRDGHAALILYLSALTGAHGSRLTRALRER
                     ESYTYSIGSKLDLSRHSGCVAISFRVSRAATASAVRAAVAAIERLSRHGFTANELRVAR
                     AYKAGRMTANLQTPRDVCHMMASLVESGRPADDIFALRDEILRLSRARLNSLARQTLSL
                     GRLVVVVVGDASRVRDGLAELPLARIATFHPSSP"
ORIGIN
        1 gcctcaaccc gtggaactcg ctgtcggcag tcacggcccc gggccacgga acgtggccca
       61 gataggaggt tgaaaatgac cgactcgacg aaggcgccag accccctgtt cgaggagttc
      121 gacatggagg tctatggacg tacctcgccg aacaacatgc cgttcttcgc gtcgttcgtg
      181 gaacttacgg acgacgagca ggcagaggtc gatgaggcgc tggcactctc cgagtagacg
      241 gcgttcgtga gtcacacgta tgccgcggag gtacagtcgc tggccacggc cggctgacac
      301 ctgaagatgc cgcggggact gtagtcgccg tcggacggtc cccgcggcgt accagtaagg
      361 actgtgtcgt gtatagttcg acgccgctgc tgttcgatac cgtctccaca ctgatcgccg
      421 gggaccgtgt cttttctggg aaccaacccg atgactgtgt cctcggtggg ctcctgacta
      481 atgtcgactc tgcctgcatg ctcggcctcg ggtacggcgc tgggctgcgg gccgtacgcg
      541 ccagcgctcc agccgctgat ttgttcgcgg tggatcgtga tccagccacc gtcggactct
      601 gccgtgcgct ctatcaagtc tacttcccct cgattcgctt ccgcatgcag gtggcggacg
      661 cagcgcagct gatgcgagac agcgtggagt gcttcgactt gatctgcgtc gatctctacg
      721 atggcgcctc gcatcccgcg ctggttttcg acgccggttt ctgggaggac gtccggtcgc
      781 gcgtcgccga caccggcgtg gtcctggtga actgctgggg gcttccggag cacctacgac
      841 cgttcgcggg tgcctcgccc cagcgggctc tcggcgaggt tctgcgtacg gtctgggagg
      901 aggtccggta cctaccggcg cggcgaaaca cgacattcgt gctgaccagt acgaacgtca
      961 gcctctgtat cgacgaggtg accgcgtggc ccggtctgtc ggccgccgac cgagccgtgc
     1021 tcgccactgc ggcgtgccgg ctgcggcacg cccgcacagt tccacaaggc gacgccggtg
     1081 gcattgcgcc agcgaggacc caggcggcga tcgacgccga gatggctcgc cggtggcccg
     1141 cgatgctgga ggtggtgggt gatgccgctg cggcggcggg attgccaccg gcagtggcct
     1201 ccggacggca agtggttctc gaccccgaga tcgccgtccc ggtgctgcga acgctgctcg
     1261 accgtgaggc tgcggaggct gcgttcattc ccaccgtcgc cgcttcgctc acgttcgaat
     1321 cggacttccg cgctggctgg ttcggggagt ggatcgctga cacacacctt gatcttgccg
     1381 agcattcgcc aaggtggttc tactccactg gcctgcctca ggcgatgtcc atgctggcga
     1441 acccgtacgc ccctgattgg ccttggcggg accggctcat cgaggccgct atgcggctca
     1501 gccgggcgga gtctgtctcg gggacttcat tggagacgac tcgtcagacg tcggcgatgg
     1561 tcgtcggcgg cccgcccgcc cggaccgacg cacccgagag cgtgcctgcg gcaccgtacc
     1621 ggaggaagga gctgaaggat gcagaagaca attgaagtag tcgacgactt ctacgtcgac
     1681 gtggaccggg tacgagagct agcgatctcc atgggcgact gggcgaatcc gaagcaggag
     1741 gcgggcatca cctacgactt tgagacatac aattcgttct tcaacgatca gctagtccgg
     1801 gtcatgcgcg acctggtggg tgcggaaatc catgttgacc ccaagcggat ggcgttcggc
     1861 gtgttcgggg tcttcggcgc cgactcgcat gtcgatttca ccacccacta tgatgagacg
     1921 gactggtccg ccatcgtcta ccttgtgccc gatgaccgct gcgaaggtgg gctttcgttc
     1981 tatcggcaca aaccctctgg tctgttcgga ccgccaactg aggagcaggc ccggcagatc
     2041 gggtacgaca gccgtgacgc gtggctgcgc gagcactact atccggacaa gatgcatccg
     2101 gagcgatggg aggagaccac ccacgtcgcc atgcggtaca accggctgat cctgctccgg
     2161 ggctcggtcc ttttccaccg tgccagcaag ggtttcggga cgacgccgga cagcggtcgc
     2221 ctgacccagc gattcttctt caacgacgcc aaccggaggg actagatgcg caagcgattg
     2281 gttgtggtcg acggtttcta cgcggaccct gacgcagtcc ggcagctcgc actgaactcg
     2341 gcattcaata gcgctgaccg gtacaactat ccaggctggc agagtaacaa ggcgcttcgc
     2401 acggacgcgc tccaggcccg catcgagggg attctcggcc gtgggatcga tgtcgacccg
     2461 gacaggctca cgtggggcgg cttccgtctc attaccgatg agactggcga aagaaccaag
     2521 gtgcatgcgg actccgccgt cgactgggct ggcatggtct atctcacgcc cgacgcgcca
     2581 tcctcggccg gcaccgggtt cttccgacac cgggagaccg ggcgtgagcg tccaccgacc
     2641 gaccgtgaag cccgccggct cggcttcgcc gacgccgacg agttcgagga acgtgtggtt
     2701 cgccgtgaca tggctgatct gtcgaagtgg gagatcgtaa gctgggtcgg gccgacttac
     2761 aaccgactgg tgttgttccg ggggtgcgag ttctaccatg ccccgctggg tggactcggt
     2821 gatcggccag caaacgcccg gctcacccac aacttcttct tcaacgagcg catcgaggtg
     2881 gggacggtcg gccgagtgct cggcaaggag gtcgtgtccg gaggtgtcag atgaggcaag
     2941 cggtgacaga ggtgccgcac tctctgcgcg gcgaggtcca catcatcgac gacttctacg
     3001 agaagcctga cgccgtacgc cgactggccc tcgacgccga gttcgtcggc ttcgacggac
     3061 gggcgaactt tccgggaggg gagagcgtca aggcgtactg gtcacctcaa caccgcgacc
     3121 gcttcgccga gctggtcggg aaggccatcg acatcgaccc atcgcagtgg gtgttcggca
     3181 agttccgact cgcgctcgcc aacgaaacgg ggcgtacgaa agtacatgtc gacttcgtcg
     3241 actggaccgc ggtgatatat ctcagcctcg acaccgactg ttccggcggg ctcggtatct
     3301 atcgacaccg tgagaccggg ttggagcggg taccgcgcgg tagcgacctc gccgcttacc
     3361 gctgttcgtc gctggaggag ttcgacagca ggtatatcat gcgtgacagc ctgcacgacg
     3421 accgctggga actgctccat caggtcgaca tccggtacaa ccggtgcgtt ctcttccgcg
     3481 gctcccggtt gttccacggc atcacccgga cgttcggcgg cgggtcggcc gacgggcggc
     3541 tgacccagaa cttcttcttc atggacgccg tcgaggggag ctccgcatga ggtcgctgct
     3601 ggacatggca cgagcggcgg tcgacctgcc ggggtcgccg gagtcatacc gattggtgac
     3661 cctggacgcg ttggggcaca ccgccagcgg gctggaactc gtcagcggaa cggaggtcgc
     3721 ggtggccgac ctgcgttcgg tactgggacg ctccgccacg gagctgtcgc gccatcggct
     3781 gtcgctgcag cggctcgggg tggtggcaat cgagggtgag gcggcgcgga cggtggccct
     3841 gcttgacggt tggcgcctca tccggtcgca cggccgtcag gccgatctgg cctctgccga
     3901 cctgcgcggc gcggatctca ccggcgcgga tctcatcgga agcgatcttt cctatgccga
     3961 ccttcagggg gcgtggctgg acagcgcaaa tctccggggt gcccgtctgc ggtgcacgga
     4021 cctgcagggc gccagcttgc gctgtgccag catgttcggc gccgacttga ccgaggccga
     4081 cctgcgccgc tgcgacctgc gccatagcga tctcagagag gcaacgtgtg ccggggtggc
     4141 gctgcgtggc gcggacacct gggcggctta cgtctgggaa gtcgacttct cctccgcgtt
     4201 tctggaggga tgcgagatcg ggcgtgccga ctaccggggt gccgactcga agccggccgg
     4261 cgcgcggtca taatgccgtt ccccgacctg acgacttctc ctggaatgga ggtatgaatg
     4321 atacgagccg tccagcacgc cacgcctccg gaggcggtgg ctgactccgg agcgggcagc
     4381 gagttgcgag tagtcctggt ctgcatgccg tgggcattgc tggacatgcc gtcgctcgcc
     4441 gtcagcaccc tggtaccagt cgcgaccgag accgccaaga cggcgcaggt ggatgtcgtc
     4501 tacgccaatc tgcggtggtg cgactacctc accgagagga ccggtgggcg tatcggggcg
     4561 gcgcagtacc agcaggtcgt agaagggtat taccacgcgc tgggcgagtg gatcttctcc
     4621 cctgcgctct acggcgcgga tgaccccatg gatacgccgt tctacctcac cgcccgtgcc
     4681 aacggcgccg acctcgacga cgcctgtgag atgtaccgcc ttgcgccggg gttcgtgatg
     4741 gctctggcaa cggagctggt ggacgccggg tacgacgtgg tgggcctgac ctccacattc
     4801 gatcagaata tgccatccct ggcactggca cgggccgtga aagagcgtag tccaagggtg
     4861 attaccgtga tgggtggggc taactgcgat gacgagcagg gagcagcgct gcaccgcagt
     4921 tttcccgccg tcgactttgt cgtacgaggt gagggggagc tgacgttcct cggctactcc
     4981 gggccgtcag cgtccgcaat gtggtcgagg cggagaccta ccaggccgcg ctagccggca
     5041 ttgacggact gtgctggcgg gccactgacg gcgagccggt gacgaaccca cagcaggcac
     5101 cggccgttcc ggtcgcggcc atcccgccgg ccgacctaac ccacttcttc gagcagttcg
     5161 cacgtgccac ctccgtgtcc gacatcgatc cgaagatcca actcgaaggt gcccggggct
     5221 gttggtgggg agagaaacac cattgcacct tctgcgggct gaatggatct ctcatgggct
     5281 tccgggccaa gagcgccgat cgactgttgg cggagatcac tgacgctgtg cggcgccacc
     5341 gcacgctgga cgtcgtattc gcggacaaca tcatggacat gggctacatc cgtgacctgg
     5401 tccccaaact cagcgagctg gactgggacc tgcggttgtt cttcgaagca aagtcgaacc
     5461 tgtcgttcgc ccagttgagg gctctcgccg atggcaaggt gacccagctc cagcccggtg
     5521 tggagtcgtt gagcagcaac gtcctgagac tcatgcgcaa gggagtggcc gcgtggcaga
     5581 acgtgcgtat gctgcgggac tgccggacgc tggcgatcta cccgggctgg aacatcctct
     5641 acggcttccc cggagagaca gcagacgact acgccgaggt ggtgcgtcag atgtcgttcc
     5701 tgcgtcatct caccccgccg gaggggtgtt accgcgtggt tctgacccgt ttcagtccgt
     5761 actttcaaga tcccagcctc ggactgatca accggggtcc gtcggcgctg ctgtcgaagg
     5821 tgtaccggct tcccaagcaa gagctggcac agctcggtta cctgttcgaa agtgagaagg
     5881 ccggtatccg cggcgaggat gtccaggcgc tggaggccgc taccagggac tggcacacca
     5941 atcacgccgg tagacggttg gtagccatac cagatgggtc agcattacgg atcgtcgacg
     6001 agcgctcccc ctccccatcg gagcatgtgc tcaaggaccc ggtggaaacc accgctttcc
     6061 acgcgacact gcggggacgt tcggccgcgg cggtacaccg gcagctcgaa gcggcgggct
     6121 tgtcggtcag cggtgagcag gtggccgatc tgatacacag ctggcatgag caaggctgga
     6181 tgttccgcga cggcgactac ttcgtcgcct tgccgaccgg gctcagctat gactgatggt
     6241 atccgacacc gagcattgac ccacgccacg gtgagatccg cgctcgtggt cgactggccc
     6301 tcgacccgcc ttcacacccg ccagcagttg gcggcatacg ccatggcggg catgcggggt
     6361 ctacagatcg ccgacactgt tgagctggac gacagcgatc cgctccccac cgcgaagttc
     6421 atccggcttc tcgttgatgc cgccggggtc ggcttgcgtg ccttctggtg tgggaccgta
     6481 gcccgtcccc tgacagcgca acttcgccat ctggacccgc cgcgcagcag atccggcgta
     6541 ccggcctggc cggttccgag gcggccgctg ttgacgatgc gtcgaggccc gggcttcgtc
     6601 caggtcgagg acctacgggt tactccgacg gcgcggtacg tgatctcaga gccggccgcc
     6661 gtgtccgtgc tcagaaccct cgagaccccc gggcccgcac cggacgagcg agcgctcgcg
     6721 acactgcagg gcttggcagc aggcggactg gtcctccagc tccgggacac ctgggtgaca
     6781 gtcgccgtgc gattcggtta cgcgcgggca tgagcgaggt acgattctcc gttctcagca
     6841 acggattacg ggtggtggcg gatcgtcatc cgatcgtgcc gctggtcgct gtgaacctgt
     6901 ggtacgcggt cggctcccga cacgagcgcc gtggcgaaca cggcctcgcc catctactgg
     6961 aacatctgat gtgcctgggc gcagcacgtc atagtgacta ccctcgtcgc ctgcaggcag
     7021 tggggggtgg ccatttcaac gccacgacaa accaggaccg gacgacatac ttccagaccg
     7081 ctccgcccga cttccttccg gaggttctgc gctatgaggc tgaccggatg gccagtctcg
     7141 gcgactcgat cgaccaggaa cggctcgact cacagcgtcg cgttgtccgc aacgaggcgc
     7201 ggcagagcca tgacctacgc ctcgccgagg cgctcctgag cgccgccttc ccggtcgggc
     7261 acccctacca ccaccctatc tgtcaggctc gggtgggaca tgggacaggg taatctcctg
     7321 aactgaccat cgagcagggc gagacaggac catcgagagc gatgaccatg agtaatgccg
     7381 atctggagcc gcgggctgag cgtcccaagc gcagggtgtt caccaacgag tacaagctgg
     7441 cgatcgtggc tgagtacgac gcgctgaccg agtcgggtgc ccgcggggca ctgctacgcc
     7501 gcgagggcct gtaccactcc catctgatcg agtggcgcaa agcgcgcgat gccggtgcgt
     7561 tgtccgggcc gacgccgagg ccgggacgca agccccagcg cagccgggag caggtcgaac
     7621 tggagcggct acgccgtgac aacgaacgaa tggccgcgga gctggcgcgt accaaagccg
     7681 cgcttgacgt ggtgggaaaa gcacacgcgc tcttggggat gctctccgag agcgcggagc
     7741 cgacgcgcga aaacaggtcg agcaggtgat cgaggaagcg tttgtcgagt tggcgccggt
     7801 caccgggatc aagcaggcat gtcggctgtt gggtaagccg cgttccagcc actaccgcag
     7861 gttacggcca gcgccagcgg gtcaggcccg gccgccgcgg ccggctccgc ccaatgcgtt
     7921 gagccgcgct gagcgggatc aggtggctgc ggtgctgcac tcggagcggt tcgccgacaa
     7981 gtccccggcg caggtgtggg ccaccctgct cgacgaaggt gtctacctgt gttccgagtc
     8041 gaccatgtac cgcatcctgc gcgaacgcgg cgaggtgcgg gagcggcgcc gccaggccca
     8101 gcacccgccg cgggtgcgcc cggagttggt cgcccacggg cccaacgaaa tttggtcctg
     8161 ggatatcacg aagttgcatg gtccgcatcg gggtgtgtac tacgacctgt acgtcatgat
     8221 cgacatctac tccaggtatg tggtcgggtg gcaggtcgcc gcccgcgagt ccggtgagct
     8281 ggctgaagag ttcatcgccg aggcgatcgg ccgcatcggt gtcgcgccca gggcggtgca
     8341 cgctgaccgt ggcactgcca tgaccagcaa accggtcgcc gcgctgccga cctgaacatt
     8401 gaccgtagcc actcccgacc gaaggtgtcg aacgacaatc cgttttcgga ggcgcagttc
     8461 aagactctca agcattgtcc ggcgttcccc ggccggtttg gatccatcgc tgacgcccgc
     8521 gcgttctgcg ccgagttctt cacctactac aaccatgatc atcgacaccg cgggatcggg
     8581 ctgcacaccc ccgcgtcggt ccacttcggc actgtcgaga agatccgcga ggaacgggcc
     8641 aaggtcctcg acgccgccta cgccgccaac ccggaccggt tccgtagccg acccacgcca
     8701 ccgaccatcc ccggagcggc atggatcaac gaaccaccag cgaccacaca gacatcgtga
     8761 gtaacacgtg tctcaacgag cttgacagct tccgccacac cacgatgggt gctatgaagc
     8821 agcttgagca gctgacgccg ggcgatgtcg ctgggtttca ccacaggtat tacggcccgg
     8881 ggaacgccac gctgactctc gtaggggata ttgatcccgc gtcggcggta cggctggccg
     8941 aggcatgctt cggcgacctg gctcctcgta tcccgccgga actgccgtcg atgtcgccag
     9001 ccggcaaccg ctcacagcag gccgttgtgc tcgaatcttc caatctgccg gacgaccgcg
     9061 ttcatctggg gtttcgaatc ccgcccgtcg gggatcccga acatgacgtg gtcaccgtct
     9121 tgggggcggt gctcggcctg ggccggggca gcttgctggc gagaagcctc gtcgaaggcg
     9181 gcttggtcct gccggccggc gagctgatga cggtctggag gtgcagcggc gacagttcac
     9241 tactggtggc tggactgagg gcggcacctg gggtcaaggc ggaggaagtg gaagcagccg
     9301 cccgcgatgt catggaggct gcctggtccc agatggtcga ggacgacctg caacgggcga
     9361 gcgcactggt ggttgcgcag gtcctgcgtc acatggagac agtcagtggg cgcgccgact
     9421 tcctcagtga gatgacgacg gtcttcggcg acccgctcgc cgctacgggg ttcgtccacc
     9481 ggatccgcgc cgtctcgctt gacgatcttc gcgataccgc tagacggtta ctcgtcagtg
     9541 acgatgcgtt cgttgtcaag tgcgtggcga ggcgggactg accagagtgg agggcacatt
     9601 tgatcgcgtg gccgcgctgc ttgtggcgag accagaccct gatggtgaac cttccccgtc
     9661 gagccaccgc cgccgccacc ctcgtagtgg agtcgggcgc tgccgccgac ccgcccggcc
     9721 gcgccggcct ggcgaccctg ctggcacgac tgcttcaagc cggtacgccg gacatggatg
     9781 ccgaactggc cgtcggtctg gaacgcttgg gagcccgatt tgctacccat gccggctggg
     9841 acgctctacg cctcaccgtg gaggcgcccc ggtcgtccct tgcagccgca gtggctctga
     9901 tggcccgcca tctgcgtacc gcgcccggag catcgctgtc gggcacccag ctacagcagc
     9961 acgccagctg ggtcgagcgg cgatgggcgc gccccgcggt cgtgggggag gccgcgttcc
    10021 gagaggcggc cctgcgtggg cggtgccgga tgcccctgga gggcacgccg gccacggcca
    10081 gggctttgga gacggtggat gtcgaacgtg ctcacggtgc cctgtcggcc gtgcaacggt
    10141 cgctgctgat ggccggggac ctgacgtcag cggagtggga cgaggtgctt ggtgccgccg
    10201 gtgaccttgg cgcaccccac gagacccgtc agcagcacgc ccggcgggtg gaggaaccac
    10261 cgttcggcca aaccgcccct cggctcgtcg tggttgatcg gccgggatcg ggttcgagcc
    10321 tggtgtccct tggatggcga ctgccgtcgt acagccgtga cgggcatgcc gcgctgatcc
    10381 tgtacctttc cgcactgacc ggtgcccacg gctcgcggct cacgcgggcg ttgcgggagc
    10441 gggaaagcta cacgtactcg attggcagca aactcgacct gtcgcgccac agcgggtgtg
    10501 tcgctatctc gtttcgggta tcccgcgccg caacagcgtc ggccgtgcgg gcggccgtcg
    10561 ccgcgatcga gcggttgtca cggcacggct tcaccgcgaa cgaacttcgc gtggcgcgcg
    10621 catacaaggc cggtaggatg acggcgaact tgcagacccc tcgagacgtg tgccacatga
    10681 tggccagtct ggtggagtcg ggccggccgg ctgacgacat cttcgcgctg cgcgacgaga
    10741 tcctacggtt gtctcgggcg cggctgaatt cgcttgcccg ccagacgctg tcattgggcc
    10801 ggctggtggt cgtggtagtc ggagacgcct cccgggtcag ggacggtctc gccgaactcc
    10861 cgctggcgcg aatcgccacc tttcacccat cctcaccgtg atgctcgccg cgacgctgac
    10921 ggggcggccc ggacccggac caccccgtca gcttgctatt tgttggtgga tgggcttatc
    10981 cgagggtcca ttgctggttg gagccgccgc tgcagatcca gagctggacc cgggagccgt
    11041 tggcggtccc gaagtcggac acgtccacgc acagcccgct aaggacgttg gcgacgctgc
    11101 cgttggagtt gaggttccat tgctggttgg ctccgccgtg gcaggtccag atgatcacgg
    11161 ccgtgccgtt ggcggtgccg ttacggcacg gtcgaactac ccgccgtcct cgtcccttac
    11221 atgggcaaga aggtct
//
