LOCUS       NZ_CP070496            10429 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Natronoglycomyces albus strain DSM 106290 chromosome, complete
            genome.
ACCESSION   NZ_CP070496
VERSION     NZ_CP070496.1
KEYWORDS    RefSeq.
SOURCE      Natronoglycomyces albus
  ORGANISM  Natronoglycomyces albus
            Bacteria; Actinomycetota; Actinomycetes; Glycomycetales;
            Glycomycetaceae; Natronoglycomyces.
REFERENCE   1  (bases 1 to 3937850)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Natronoglycomyces albus gen. nov., sp. nov, a haloalkaliphilic
            actinobacterium from a soda solonchak soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3937850)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) Bioinformatics, Leibniz Institute DSMZ,
            Inhoffenstr. 7B, Braunschweig 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-MAY-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name :: 871_Haloglycomyces_alkaliphilus_DSM106290
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 620.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016925535.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 05:55:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,382
            Genes (coding)                    :: 3,339
            CDSs (with protein)               :: 3,339
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 2 of 43
            Pseudo Genes (incomplete)         :: 40 of 43
            Pseudo Genes (internal stop)      :: 2 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:08:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2891640
            Orig. end                         :: 2902069
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070496.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10429
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="11"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10429
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10429
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5429](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5429)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    292..302
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.05"
                     /tool="antismash"
     gene            complement(330..1616)
                     /gene="eno"
                     /locus_tag="JQS30_RS12445"
                     /old_locus_tag="JQS30_12435"
     CDS             complement(330..1616)
                     /EC_number="4.2.1.11"
                     /GO_function="GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="eno"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1268: mandelate racemase/muconate lactonizing enzyme"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551095.1"
                     /locus_tag="JQS30_RS12445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12435"
                     /product="phosphopyruvate hydratase"
                     /protein_id="WP_213170571.1"
                     /transl_table=11
                     /translation="MALIDTIRAREILDSRGNPTVEVEVLLSDRTLHRAAVPSGASTGA
                     FEAVELRDNDPNRYLGKGVEQAIENINGPITEALIGFEASDQRFIDQKLLELDGTADKS
                     NLGANAILGVSMAVAKAAATSANLPLYAYLGGPNAHILPVPMMNILNGGAHADSNVDIQ
                     EFMIAPIGAATFAEAVRYGAEVYHALKAVLKSQGLSTGLGDEGGFAPDLSSNRAALDLI
                     VEAIQKAGFTVGTDVALAMDAAASEFYKDGAYVFEGEKKTSQDMADYYEQLLADYPIVS
                     IEDPLDEEDWAGWASLTAKIGDKVQLVGDDIFVTNPERIRRGIAEKSANGLLVKVNQIG
                     SLAETFDAMDLAHRAGFGCMMSHRSGETEDTTIADLAVATGCGQIKAGAPARSERVAKY
                     NQLLRIEGDLGEAARYAGAGAFPKFTPQS"
     gene            2138..2797
                     /locus_tag="JQS30_RS12450"
                     /old_locus_tag="JQS30_12440"
     CDS             2138..2797
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JQS30_RS12450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JQS30_12440"
                     /product="hypothetical protein"
                     /protein_id="WP_213170572.1"
                     /transl_table=11
                     /translation="MSAFPRAGRLVSIVALAAVATLSACSDPNGNGGGDPRQDYQVLDE
                     LSKRATSGIDHSYTADYMVGSGSDQLRLVRDAEGDRMAIGLMDELHVFTPAYTAYCQAG
                     ECTREDEHGLDLAEWMRDLTPQLLPAQWDASYWLSALDADSSADIDYHDTRLAGELADC
                     IEVAGAVESPVSAFELCLTTHGVIASLTAVVDGEELRVKLTSIANSVDEEYFTQVK"
     gene            complement(2914..3825)
                     /gene="thpD"
                     /locus_tag="JQS30_RS12455"
                     /old_locus_tag="JQS30_12445"
     CDS             complement(2914..3825)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566348.1"
                     /locus_tag="JQS30_RS12455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12445"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_213170573.1"
                     /sec_met_domain="PhyH (E-value: 4.3e-28, bitscore: 96.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQDLYPTRVSHRPAMIPRKLDPTVYGQAEDGPLNRQQLTGHSQ
                     DGFTSIDKLLTDSELRSAQQELRSLLTDPRLDGDERVVREKNSQEIRSIFDIHHISEEF
                     RRLIDDERLAGVARQILGSQVYIHQSRVNYKPGFGGNSFYWHSDFETWHAEDGMPRMRA
                     VSLSIALTDNYDYNGSLMIMPGSHRTFVSCVGDTPEGYYRTSLQEQEIGTPDTASLRAL
                     ADRHGIRQFTGPAGSGLWFDANCMHGSNGNITPYPRSNLFIVFNSVENALVEPFAAPQP
                     RPTYLGARTVEPIGSPTKAMAS"
     gene            complement(4102..5004)
                     /gene="thpD"
                     /locus_tag="JQS30_RS12460"
                     /old_locus_tag="JQS30_12450"
     CDS             complement(4102..5004)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013021015.1"
                     /locus_tag="JQS30_RS12460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12450"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_213170574.1"
                     /sec_met_domain="PhyH (E-value: 8.1e-29, bitscore: 99.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVHDMYPTRGSREPALLYRHDDPTVYGKPEDGMLTAEQLAHHEA
                     AGYTDIEQLLNDAEVEKYAEEMDRLLQETQKSATSADQGDDEHVVREDERQEVLSIFDV
                     HRNSELFAELISDERVAGVARQILGSEVYIHHSRISYKPGLVGTPFYWHSDFETWHAED
                     GMPRMRAVTLSLALTDNTEHNGSVMVMPGSHKVFVSCVGDTPDEHYKSALQDQRIGTPD
                     DVSLGMLAEKYGIQQLNGPAGSATWIDCNLMRGTNGNITPFARANLVVAFNSVENECGE
                     PYRADEARPDFLARDVEPI"
     gene            complement(5001..5429)
                     /locus_tag="JQS30_RS12465"
                     /old_locus_tag="JQS30_12455"
     CDS             complement(5001..5429)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013021014.1"
                     /locus_tag="JQS30_RS12465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12455"
                     /product="ectoine synthase"
                     /protein_id="WP_213170575.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.6e-52, bitscore:
                     172.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRRLEDLVGSENDVLGNDRDKAEGTWRSRRLLLAKDKVGFSLH
                     DTVLYAGTSTTMCYANHIEAVYVIEGAGELADHETGETHKLAPGTMYLLNGGERHTLTA
                     HEDLRCVCVFNPPVTGREVHDENGVYPLLTEDDEEATS"
     gene            complement(5430..6686)
                     /gene="ectB"
                     /locus_tag="JQS30_RS12470"
                     /old_locus_tag="JQS30_12460"
     CDS             complement(5430..6686)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 6.2e-74.
                     Score: 241.0. Matches aSDomain:
                     nrpspksdomains_JQS30_RS12470_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290748.1"
                     /locus_tag="JQS30_RS12470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12460"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_213170576.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.9e-76, bitscore:
                     253.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVFEKVESEVRSYCRSWPTVFTTASGSRLKDESGKSYIDFFAGA
                     GSLNYGHNHPVIKQALLDYLAEDNIIHGLDKFTVAKRDFLETFDEIILTPRQLDYKVMF
                     PGPAGNNAVEAALKLARKYTGRETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLNNV
                     ATMPFDNYMDGQMPDFLWLRSLLEDSGSGLDQPAAVIVETVQGEGGINVASDEWLRGLQ
                     EVCREYGMLLIVDDIQMGCGRTGPFFSFERAGIQPDIVTISKSLSGLGMPFALTMMKRE
                     LDVWEPGEHNGTFRGFNPAFVTATAALEHFWRTDELANSVHQTGEWLDGQLKELITEHA
                     SLNMSTRGQGLARGLVFENPEHASKVCSEVFQRGLLMESAGAEDEVVKFLPPLTTSDAE
                     FKEGIGILADAVEAVGRNS"
     aSDomain        complement(5616..6620)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JQS30_RS12470_Aminotran_3.1"
                     /evalue="6.20E-74"
                     /label="JQS30_RS12470_Aminotran_3.1"
                     /locus_tag="JQS30_RS12470"
                     /protein_end="357"
                     /protein_start="22"
                     /score="241.0"
                     /tool="antismash"
                     /translation="TTASGSRLKDESGKSYIDFFAGAGSLNYGHNHPVIKQALLDYLAE
                     DNIIHGLDKFTVAKRDFLETFDEIILTPRQLDYKVMFPGPAGNNAVEAALKLARKYTGR
                     ETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLNNVATMPFDNYMDGQMPDFLWLRSL
                     LEDSGSGLDQPAAVIVETVQGEGGINVASDEWLRGLQEVCREYGMLLIVDDIQMGCGRT
                     GPFFSFERAGIQPDIVTISKSLSGLGMPFALTMMKRELDVWEPGEHNGTFRGFNPAFVT
                     ATAALEHFWRTDELANSVHQTGEWLDGQLKELITEHASLNMSTRGQGLARGLVF"
     gene            complement(6807..7574)
                     /gene="ectA"
                     /locus_tag="JQS30_RS12475"
                     /old_locus_tag="JQS30_12465"
     CDS             complement(6807..7574)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /locus_tag="JQS30_RS12475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12465"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_213170577.1"
                     /transl_table=11
                     /translation="MPLGENSDTGKGAQHYTADRQTAYSSADEFGDEAGEPEKRPTSPR
                     NQGVENDATASASYGGTSRGTVHQNPAFENPAVQSGAEPGPNSTATDQPQLTFDQPTAD
                     DGAAIWHLVGEIGNLDLNSRYFYVLWGRDFAADSVVVRDGDRIAGFIVGYRRPESVDTL
                     FVWQVAVSPHYRRQGLARRMLDHLWNRLRPLGVNFVESTVTPDNKASMRLFESFAEGHN
                     AELTRDVLFSEQMLGEGHEPEVLHRIGPVPSDA"
     misc_feature    7671..7688
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(7738..7751)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.79"
                     /tool="antismash"
     gene            complement(7877..9004)
                     /locus_tag="JQS30_RS12480"
                     /old_locus_tag="JQS30_12470"
     CDS             complement(7877..9004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018810744.1"
                     /locus_tag="JQS30_RS12480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_12470"
                     /product="MazG family protein"
                     /protein_id="WP_213170578.1"
                     /transl_table=11
                     /translation="MTPTDADDARRPSTHDSSLSEGGAASRADAAGPGLKTVVHWLVTS
                     PRLPAGLLSAEAWDTVRSSVTVVAPAEHPQIEALCASGVDVALAGSAAEALELLEDGGV
                     WLASTAPVDSDSDKSIDAVLVEEFAASTASGEISLEILYGSWDPPGARLLDLVEIIDQL
                     RVDCPWQSAQGHRSLAPYLLEETYEALDAITDENRDALREELGDVLFQPLLHARIASEH
                     ASEPFSIDDVASDLIDKLIRRHPHVFGEADPANLFQLWDEAKAAEKPERRDDPATGVSE
                     QLPALAYAAKVMDRFAMAGRPIEVPVLPESASRLMLDEVGTGIMLLAVIRVALQNGIDP
                     ELALRNTMRFVVASGGQVRRLPGNDGDGDSGEQHQ"
     misc_feature    complement(9005..9021)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.09"
                     /tool="antismash"
     gene            complement(9010..9669)
                     /locus_tag="JQS30_RS12485"
                     /old_locus_tag="JQS30_12475"
     CDS             complement(9010..9669)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JQS30_RS12485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JQS30_12475"
                     /product="hypothetical protein"
                     /protein_id="WP_213170579.1"
                     /transl_table=11
                     /translation="MRHTSATKRRLLTMAGLGLTATLALTGCQVEQGAAVFVGDERIDE
                     EVVDSYIDEHLQARGFDLAEQGHADFSEERQSLVAAVTWVELGRQMGLDSEGILTPAWS
                     DPGTIEQLFTEESVYRGQIVSALLEDHIPDQITDEHLQSVQNDLTEHGLHGEINPEYQH
                     LPLLVALSLASEDLSEQVGDYDIKINPRYGDVRLELLPEEILAESPELAGLYVLEF"
     misc_feature    complement(9849..9864)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.6"
                     /tool="antismash"
     misc_feature    9851..9869
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.87"
                     /tool="antismash"
ORIGIN
        1 ccccgggacg gttggggcgg cggcgtgtac tcacgagacc tagcttatcg aaggtggagg
       61 gcgcatggag cgaggtcggc tgccgggggc gcaaccggcg catcatcggc gggtctcacc
      121 ggcgaggtta tccactgccg agcagtcgag ccctccggtg tgctcgtgcg ggcgcccgtt
      181 gagatggcgt cgtgacggtt gccgttgcac ggaggtgggc ccgggtgagt tatctcccgg
      241 gcccactcgc gtggtgtgac gagaagcctc ttgggttcag gcttagctca ttgccatgtc
      301 acttcatgac cggcgtaagg ccatggagcc tagctttgcg gcgtgaactt ggggaaggca
      361 ccggcgccgg cgtagcgggc cgcctcgccc aggtctccct ctatgcgtag caactggttg
      421 tatttggcga ctcgttccga gcgcgccgga gcgccggcct tgatctgtcc acatccggtg
      481 gccacggcca ggtcggcaat cgtggtgtct tcggtctcgc ccgaacggtg gctcatcatg
      541 cagccgaaac cagcccggtg ggccaggtcc atcgcgtcga atgtctcggc cagcgaaccg
      601 atctggttaa ccttcactag cagtccgttc gcgcttttct cggcgatgcc gcggcggatg
      661 cgctcggggt tggtgacaaa gatgtcatct cccaccagct gcaccttgtc gccgatcttc
      721 gctgtcaggc tcgcccagcc ggcccagtct tcctcgtcca gtgggtcctc gatcgacacg
      781 atcgggtagt ccgccagcag ctgctcgtag tagtcggcca tgtcctggga ggttttcttc
      841 tcgccctcaa agacgtaggc gccgtccttg taaaactccg aggcggccgc gtccatagcc
      901 agcgcgacat cggttccgac agtgaatccg gctttttgga tggcttcgac gatgaggtcc
      961 agcgccgccc ggtttgagga caggtccggc gcgaagccac cttcgtcgcc cagcccggtc
     1021 gagagtccct gactcttcag gactgctttg agggcgtggt agacctcggc tccatagcga
     1081 acggcctctg cgaaggtggc ggcgccgatc ggcgcgatca tgaattcttg gatgtcgacg
     1141 ttggagtcgg cgtgtgcccc gccgttgagg atgttcatca tcgggacggg gagaatgtgc
     1201 gcgttcggtc cgcccaagta ggcgtacagc ggcaggttgg cactggtcgc ggcggccttc
     1261 gccaccgcca tcgacacgcc caggatcgcg tttgctccca ggttggattt gtcggcggtg
     1321 ccgtccagtt ccagcagctt ctggtcaatg aaacgctggt cagaggcctc gaagccgata
     1381 agagcctcgg tgatggggcc gttgatgttc tcgatagcct gctcgacgcc cttgccgaga
     1441 taacggttcg ggtcgttgtc gcgcagctcg acggcctcga atgcgccagt ggaggcgcct
     1501 gaggggaccg cggcccggtg cagagtgcgg tcggagagca gaacttccac ctcgacggtg
     1561 ggattgccgc gcgaatccag gatttcccga gctcgaatgg tgtcgatgag agccactatt
     1621 tctcctaaga atagggtcaa tttaggtcga gccagcccca ctctgcgctg gcctcatgct
     1681 cgcccacacc cgatttgcga aatggttgac gcgggcgcgg ttcgggcgag atcacttcgc
     1741 cctagactac gctgcggccg gtggcactcc cgggttaagg tggggaaaaa tcactgattg
     1801 atcaccatga ggctgatgtt tacgcgggcg aacctgcgcc atgacgtcgc cattggattc
     1861 cgtgccgcca ctttgtggcc ggtgagactt tgtcggcgac ctgagccgcc attgggccgc
     1921 ggaaacatga ctgagaactc acacctttcc ggcgttggca accgagcgct gtttggatcg
     1981 cgtcatgttt gtggaccggt taaacggccg cacacggcca gttcactcga cgctgggtat
     2041 gtcgggggcc tggcgtcgtt tgcgtgaatg tccaatcgca cttgtctacg tcgcaccgct
     2101 gtcgacctcg atcgtcccca cacttggagc ttcccctatg tctgcattcc cccgggctgg
     2161 ccgcttggtg tccattgtcg cgttggcggc ggtggctacc ctcagcgcct gttctgaccc
     2221 caatggcaat ggcgggggcg atccgcgcca ggattatcaa gtcctggatg agctaagcaa
     2281 acgagcaacg agtggaatag accactccta taccgctgac tacatggtgg gttctggtag
     2341 tgaccagctg cgcctggtgc gtgacgccga gggtgaccgg atggcgatag gtctgatgga
     2401 tgaactgcat gtgtttaccc cggcctatac cgcgtattgt caggcgggcg aatgcactcg
     2461 tgaggatgag catggcctgg acctagccga atggatgcgc gacctcaccc ctcagttgct
     2521 acctgcccag tgggacgcca gctactggct gtcagccctg gacgcggact ccagtgcgga
     2581 tatcgattac cacgacacac gtcttgctgg tgagctggct gactgcatcg aggtggcggg
     2641 agccgtggag tcgccggtga gcgcctttga actgtgtttg acgacccatg gcgtcatcgc
     2701 cagcctcacg gcggtagtcg atggtgagga gctgcgagtc aaattgacta gcatcgccaa
     2761 ttccgtcgac gaagaatact tcacgcaggt caagtaaggt ttgaagcccc agccggtggg
     2821 gtgggccagg agagcctggc ccaccccacc ggcttttctt ccccggtttt ggcgacgcta
     2881 gccgcgttca gcaccacagt ggcgcgtgcg ttctcacgag gccatggcct tcgtcgggct
     2941 gccgatcggc tccactgtgc gcgcccctag ataggtcggt cgaggctgcg gggcggcgaa
     3001 gggttccacc agcgcgttct cgacgctgtt gaagacgatg aaaaggtttg agcgcggata
     3061 tggcgtgatg ttgccgttgg aaccatgcat gcagttggca tcgaaccaca gccccgatcc
     3121 tgctggcccg gtgaattgtc gaatgccgtg gcggtcggcc agggcccgca acgaggcggt
     3181 gtctggggtt ccgatctcct gctcttgcag cgaggtgcgg tagtagccct caggggtgtc
     3241 accgacgcag gagacgaacg tgcggtggct gcccggcatg atcatgagcg acccgttgta
     3301 gtcgtagttg tccgtcagcg ctatcgacag actgaccgca cgcatgcgtg gcataccgtc
     3361 ctcggcgtgc caggtttcga aatcggagtg ccagtaaaag gagtttccgc cgaatccggg
     3421 cttgtagttg acgcggcttt ggtgaatgta cacctgtgaa cccaggattt gcctcgccac
     3481 gcccgcgagc cgctcatcgt cgatgaggcg gcggaactcc tcggaaatat ggtgaatgtc
     3541 gaaaatcgag cggatttcct gggagttctt ctctcgcacc actcgctcgt caccgtcgag
     3601 cctggggtca gtgagaaggc ttcgcaactc ctgttgggca gaacgtagtt cggagtcggt
     3661 cagcagcttg tcgatgctag tgaaaccgtc ttgggagtgg ccggtgagtt gttgccgatt
     3721 tagggggccg tcttcagcct gcccgtagac ggttgggtcg agtttgcgcg ggatcattgc
     3781 cggtcggtgg cttactcgcg ttggataaag gtcttgagta gtcatcgtgc tctcctcaaa
     3841 agtctttggt cacggtcgcg tgtttgccgt tgtgtcctgg ttcctccttt cgacgtcgtt
     3901 tgcgctgtca ctgtggagtt atatcggatg gcactgaggt gacatcggtc tatctacact
     3961 gtcacacacg ccgaagggcc ccactcggtc ttatccacag cagtgaagat tgctgctcaa
     4021 agaccgggta ggaccctcgg tatccctgac tggctctcct gggcgccacc gcgcccagta
     4081 cgtgccatat ccggttatgt actaaattgg ctcgacgtca cgggcgagaa agtccgggcg
     4141 ggcctcatcg gctcgatagg gctcgccaca ttcgttctcg acgctattga acgccaccac
     4201 caagttggca cgggcaaacg gcgtgatgtt gccgttggtg ccgcgcatca gattgcagtc
     4261 aatccacgtt gccgaacctg ccgggccgtt gagctgttgg ataccgtact tctcagccag
     4321 catccccaaa ctcacgtcgt cgggcgtccc gatccgctga tcttgcaacg cggacttgta
     4381 gtgttcatcg ggcgtgtctc cgacgcagga gacgaacacc ttatgcgaac cgggcatgac
     4441 catcaccgaa ccgttgtgct cggtgttgtc agtcaaggcc aacgacaacg tcaccgcgcg
     4501 catacgcggc atgccgtctt cggcgtgcca agtctcaaag tcagagtgcc agtagaacgg
     4561 cgtaccgacc aaacctggct tatagctaat gcggctgtgg tggatgtaca cctcagagcc
     4621 cagaatctgc ctggccactc cggcgacgcg ctcatcggag atcagctcgg cgaaaagctc
     4681 agagttgcgg tgcacatcga agatcgaaag cacctcttgg cgttcgtcct cacgcaccac
     4741 atgctcatcg tcaccctgat cggcgctggt ggcagacttc tgggtttctt ggaggagacg
     4801 gtccatctcc tcggcgtact tttccacctc ggcgtcattg agcagttgtt caatgtcggt
     4861 gtaacctgcg gcctcgtgat gagccagctg ttcagcggtc aacatcccgt cttcgggctt
     4921 gccgtagacg gtcgggtcat cgtgccgata cagcagtgct ggctcgcggc tgccacgggt
     4981 ggggtacata tcgtggacgg tcatgaggtg gcctcctcgt cgtcctctgt tagcagcggg
     5041 taaacgccgt tttcgtcgtg cacctccctt cccgttacag gtgggttgaa tacacagacg
     5101 caacgcagat cctcgtgcgc ggtcagcgtg tggcgttcac ccccgttgag cagatacatc
     5161 gttcccgggg ccaacttgtg tgtttccccg gtctcgtgat cggcaagctc gccagcgcct
     5221 tcaatgacgt agacggcctc gatgtggttg gcatagcaca tggtcgtgga ggttcccgcg
     5281 tatagcacag tgtcgtgcag cgagaatccg accttgtcct tggccagtag tagacgccgg
     5341 gagcgccagg tgccctcggc cttgtcgcgg tcgttaccca gaacatcgtt ctcggagccg
     5401 acgaggtctt ccaaacgacg gacgatcatc taggagttcc tccctacggc ttcgacggcg
     5461 tcggcaagga tgccgatgcc ttccttgaac tcggcgtccg atgtcgtcag cggcggcagg
     5521 aacttcacga cctcgtcttc ggctccggcc gactccatga gaagtccgcg ctggaagacc
     5581 tcggaacaca ccttggaggc gtgctcgggg ttttcgaaga ccagtccgcg cgccaggcct
     5641 tgtccacggg tggacatgtt gaggctggcg tgttcggtga tgagttcctt cagctggccg
     5701 tcgagccatt caccggtttg gtgaacgctg ttggctagct cgtcggtgcg ccagaagtgc
     5761 tccagcgccg ccgtggcggt gacgaaggcg gggttgaatc cgcggaaggt tccgttgtgt
     5821 tcgcccggct cccatacgtc cagttcccgc ttcatcatgg tcagggcaaa tggcataccc
     5881 agaccggaga gggacttgga gatggtgacg atgtcgggtt gaataccggc gcgttcgaag
     5941 gagaagaacg gcccggtgcg tccgcaaccc atttggatgt cgtccacgat caggagcatg
     6001 ccgtattcgc ggcacacttc ctgtagccct cgcagccatt cgtccgaggc gacgttgatg
     6061 ccgccctcgc cctgaacggt ttcgacgatg acagccgctg gctggtccag gcctgaaccg
     6121 gagtcctcca gcaggcttcg tagccatagg aagtcgggca tctgcccgtc catgtagttg
     6181 tcgaagggca tggtggccac gttgttcagc ggcacacccg cgcctccacg cttcatggag
     6241 ttaccggtga cggcgagcgc ccccagagtc atcccgtgga agccgttggt gaagctgatg
     6301 actgtctcgc ggccggtgta tttacgggcg agtttcagtg ccgcttcgac ggcgttgttg
     6361 ccagccggtc ctgggaacat aaccttgtag tcgagctgac gcggagtcag gatgatctcg
     6421 tcgaaggtct ccaggaaatc gcgcttggcg acagtgaact tgtccaggcc gtggatgatg
     6481 ttgtcctcgg ccaggtagtc caggagtgct tgtttgatga ccgggtggtt gtgcccgtag
     6541 ttgagcgaac cggccccggc aaagaaatcg atgtatgatt tgccgctttc gtcctttaga
     6601 cggctgcccg acgcggtggt gaagacggtc ggccagctac ggcagtatga acggacttct
     6661 gattcgactt tttcgaatac ctgcatagtc tcttagggtc ggcctatcga gctgtctctg
     6721 cttcccaggg cagtgcaagc ggttgcctgg accgccgctg agcgcttacc cgggcggcaa
     6781 aacgtgggac ccgggtaggg cgatgtttaa gcgtcggacg gaaccgggcc gatcctatgc
     6841 aggacctccg gttcatgccc ttcgcctagc atctgctcag agaacagcac gtcacgcgta
     6901 agttcggcgt tatgcccttc ggcaaacgac tcaaacagcc gcatggacgc tttgttgtcc
     6961 ggagtcaccg tcgattccac gaagttgact cctagcgggc gtagccggtt ccacaagtgg
     7021 tcgagcatac gccgcgccaa accttggcga cgatagtgag gagacaccgc aacttgccag
     7081 acgaacaggg tgtcgaccga ttcagggcgt cggtatccga cgatgaagcc cgcgatgcgg
     7141 tctccgtcgc ggaccaccac ggagtcggcg gcgaaatcgc ggccccacag cacgtagaaa
     7201 taacgtgagt tgaggtcgag attgccgatt tctccgacaa gatgccagat agctgctcca
     7261 tcgtcagcgg ttggctgatc gaaggtgagc tgaggctggt cggtcgccgt tgagttcggg
     7321 cctggttcgg caccgctttg gacggctggg ttctcaaagg cggggttctg gtgaaccgtg
     7381 cctctagaag tgccgccata ggaggcactg gcagttgcgt cgttctccac gccctggttc
     7441 ctcggtgagg tagggcgctt ttcgggctcg cctgcttcgt caccgaactc gtcggcggag
     7501 gaataggcgg tctggcgatc agcggtgtag tgctgagcgc cttttccggt gtcactattt
     7561 tcgccaagtg gcatgtgtac gaaaatagca aatttttgcc cgctgggtaa cccctagact
     7621 cagagttgtc gagtcaaaca cacttatcgg tatgcgattt gaccagcaaa aagttatttc
     7681 ttcgttatgg gtctaaaatg gaacgaatgc tcggcgagca ctattcacat acatgcataa
     7741 gattcgatct acagtgattg cactggaact tcgccgtcct ttcgggcgca ccagagtgat
     7801 ctctagacag cccggcgaaa ggtctccgat gccactcagt ggcgtcacgc cgtggacaga
     7861 ttggctatga tgcagctcac tgatgctgct cgccactatc gccatcccca tcgttgccgg
     7921 gcaggcgacg cacctgcccc ccagaggcca ccacaaaacg catcgtattg cgcaaggcca
     7981 gctcagggtc gatgccgttc tgcagggcaa cccgaatcac cgccagcaac atgatccccg
     8041 tacccacctc gtcgagcatc aatcgcgaag ccgactcggg caagaccgga acctcaatgg
     8101 gccgccccgc catcgcaaag cgatccatga ccttcgccgc ataagccaaa gcgggcagct
     8161 gctcagagac cccagtagcc ggatcgtccc tacgctcagg tttctcggcg gccttggcct
     8221 catcccacag ctggaacaaa ttcgccggat cggcctcgcc aaaaacgtga ggatggcgac
     8281 gaatcagctt atcgatgagg tcagaggcca catcgtcgat actgaacggc tccgaagcat
     8341 gctccgaggc gatccgagcg tgcaacagcg gctgaaacaa cacgtcgccc agctcctcgc
     8401 gcaaggcgtc ccggttctca tcggtgatag cgtcaagcgc ctcgtaggtc tcctccagca
     8461 aatacggagc cagagaccga tgcccttgcg cactctgcca agggcagtca acacgcaact
     8521 ggtcaatgat ctccaccaag tccaacaacc gcgcccccgg cggatcccaa gaaccataga
     8581 ggatctccaa gctaatctca ccggatgcgg tgctcgcggc gaactcctcg accaaaaccg
     8641 cgtcaatgct cttgtcggaa tcggaatcca ccggagcggt gctagccagc cacacgccac
     8701 catcttccag cagctccaac gcctccgccg ctgagcctgc cagcgccacg tccactcccg
     8761 aggcgcacaa tgcctcaatc tgaggatgtt cagccggcgc tacgaccgtc acggaggagc
     8821 gaaccgtgtc ccacgcctcg gcagacagca ggcccgcagg caaacgcggg gaggtgacca
     8881 gccagtggac gaccgtcttc agcccaggcc cggccgcgtc cgcacgcgag gccgcgccac
     8941 cttcggacaa cgacgaatcg tgagtcgaag ggcggcgcgc gtcatcggcg tcggtgggag
     9001 tcatcggtcc taaaactcca agacgtacag gccagccagc tcagggcttt cggcaaggat
     9061 ctcctcgggc agaagctcca gccgcacatc gccgtaacga gggttgatct tgatgtcata
     9121 gtcgccgact tgctcactca ggtcctcgct ggccagggag agcgccacca aaagcggaag
     9181 atgctggtat tcggggttga tctccccgtg cagaccatgc tcggtcaagt cgttctgcac
     9241 cgactgcaag tgttcgtcgg tgatctggtc gggaatgtgg tcttccagca gggcggaaac
     9301 aatctgcccc cgatatacgg actcttcagt gaacagctgc tcaatcgtgc cagggtccga
     9361 ccacgcgggc gtgagaatcc cctccgagtc caaacccatc tggcggccca attccaccca
     9421 agtgacggcc gcgactaggc tctgccgctc ttcggaaaag tccgcgtggc cctgctcggc
     9481 cagatcgaaa ccgcgcgctt ggaggtgctc gtcgatatag ctgtcgacga cttcctcatc
     9541 aatacgctca tcgccgacga agaccgccgc gccctgttcg acctgacatc cggtcaaggc
     9601 gagggtggcg gtgagtccga ggcccgccat ggtcaggagt ctgcgcttgg tcgcggaagt
     9661 gtgccgcatc tgaaccgcct ttgtactcgt gcacgtgttt gcgagtgcac tgattttgtg
     9721 aggtgcattg ccgtcgatga acacatcagg gccgggctcg ggccacttag ccccgcctcg
     9781 tctggaggtg aggcgcgaca gccgacggca ccgtttggat gagccctgtg tgtatcgggg
     9841 ttctgtgctg tgagggtagt cgcacctact gcgctcgtcc cctgaggggc cgccaccggt
     9901 gaggatctca cgctttaatg ccccgatcac caagatcggt gccgcgtcgt gagttcacca
     9961 gtgatacgtc caaggtgcgg ggtcatacga tcggtggcga ttccatttta tcccactgtg
    10021 ggcattgagc gcctggcatt attccgccgc cgtctgcgct cagctcggcc gcccatcacc
    10081 gtgccgccgt tagttggcca caggttgact gtggtcgctg ggctggggaa acacatcggt
    10141 gatgagatcg gcgcaccatt gcaagagcgg caggccttcc attggcgtgc cacccacccg
    10201 ggccgtcgac gggcgcttga tggaaatgag cccggcggtg tccttgtaca cggcttccgg
    10261 gtagtgccgt ttcagccgca tttgcttgga atcgggcagc gtcacccgtg agaaacgcaa
    10321 gttgcgtccc tggagtgcca cctcttgcag cccgaagcga cgggccaaaa ggcggaagtt
    10381 cgccacatgc agcaagttct ccaccgagtc gggcagggct ccgtagcgg
//
