LOCUS       NZ_CP070496            14018 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Natronoglycomyces albus strain DSM 106290 chromosome, complete
            genome.
ACCESSION   NZ_CP070496
VERSION     NZ_CP070496.1
KEYWORDS    RefSeq.
SOURCE      Natronoglycomyces albus
  ORGANISM  Natronoglycomyces albus
            Bacteria; Actinomycetota; Actinomycetes; Glycomycetales;
            Glycomycetaceae; Natronoglycomyces.
REFERENCE   1  (bases 1 to 3937850)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Natronoglycomyces albus gen. nov., sp. nov, a haloalkaliphilic
            actinobacterium from a soda solonchak soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3937850)
  AUTHORS   Sorokin,D.Y., Khijniak,T.V., Zakharycheva,A.P., Boueva,O.V.,
            Ariskina,E.V., Hahnke,R.L., Bunk,B., Sproer,C., Schumann,P.,
            Evtushenko,L.I. and Kublanov,I.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2021) Bioinformatics, Leibniz Institute DSMZ,
            Inhoffenstr. 7B, Braunschweig 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-MAY-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name :: 871_Haloglycomyces_alkaliphilus_DSM106290
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 620.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016925535.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 05:55:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,443
            CDSs (total)                      :: 3,382
            Genes (coding)                    :: 3,339
            CDSs (with protein)               :: 3,339
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 2 of 43
            Pseudo Genes (incomplete)         :: 40 of 43
            Pseudo Genes (internal stop)      :: 2 of 43
            Pseudo Genes (multiple problems)  :: 1 of 43
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:08:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2383326
            Orig. end                         :: 2397344
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070496.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..14018
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..14018
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14018
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:9018](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..9018)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1520..2710
                     /locus_tag="JQS30_RS10120"
                     /old_locus_tag="JQS30_10110"
     CDS             1520..2710
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="JQS30_RS10120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10110"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_213170166.1"
                     /transl_table=11
                     /translation="MLSTFGFDKATESVYLAMLENPSAGVTDLASILNQTEETIRASLD
                     TLTESALVTAENERISPVDPEIGLAALLSRQQTQVSMMQQQVEASRLAIAQLLSKYGAK
                     SSENTNVETIIGLAAIHERIKELSDACQAEALILAPTGRLQSKTVERSEPTDQAMLNRG
                     VRMKTVYLDSVRNDSYTMEHIEIEMAAGGEVRTAPSLPLRMIIFDRQTALIPLDDSTCE
                     IGALALRNRVIIQALVTLFMTVWSEAKPATKQRTPRCDPLTDQEKHILRLWAQGNTDAS
                     AARKMRVSIRTVRRLSDKLTARLGARSRFQLGALAVSQSLIGEADIVLYAHFGLNCRME
                     LFTKNGHNYVGVRASARTPTLMLCRRFTNRPTRFHSCARQPDMVPPLLPHLALSRR"
     gene            complement(3434..3688)
                     /locus_tag="JQS30_RS10125"
                     /old_locus_tag="JQS30_10115"
     CDS             complement(3434..3688)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JQS30_RS10125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JQS30_10115"
                     /product="hypothetical protein"
                     /protein_id="WP_213170167.1"
                     /transl_table=11
                     /translation="MQMIVCLLKGAFQDLEPDCIKRSVLSAQSPQDLVEHVYVRVSERV
                     EVVVYTGVEGHQEAQVRIEQLIRRAVAPLDDYTCWALEM"
     gene            complement(3947..4912)
                     /locus_tag="JQS30_RS10130"
                     /old_locus_tag="JQS30_10120"
     CDS             complement(3947..4912)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /locus_tag="JQS30_RS10130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10120"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_213170168.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.3e-08, bitscore:
                     31.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHVCVIGGTRFVGKLLVQTLIDEGHDVTIITRGQSADDLGDSVR
                     RLHADATDAEQLTSAVAGRSFDAVIHQVCYTPNAALAAVAAFGDRVGKYVMTSTIEVYN
                     PSSFGQAAPQIGGFALEEELDTRTYGYDTELPWHEQAYIAQNYGEGKRQAEAALWENAP
                     VPVVIPRAAHVFAEVGDFTGRFQFHVDRVLARQPILTHVDAGLTSVVLALDVARFLAWA
                     ATSTVEGTYNVCSVKPVSVADMCAVIEDVSGVKAIIEERDQLSDAERRSPFSYPSDFTM
                     SPHKAMEAGFAFMPTHRWLSRLASVAVANGRARHILERAG"
     gene            complement(5001..6788)
                     /locus_tag="JQS30_RS10135"
                     /old_locus_tag="JQS30_10125"
     CDS             complement(5001..6788)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="JQS30_RS10135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10125"
                     /product="YcaO-like family protein"
                     /protein_id="WP_213170169.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-28, bitscore: 97.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEVDSLAIYAVGTESLVVAPLGRQWRIDASPDDVLSSTVLAHLA
                     ALAGPAVRIRFDPHDVLVSSTDELLGGVHRATTGGRRAEDVTGWFFAGHWYRMRRDHAA
                     LRTAVDCLRATIPDADLAAVLAGPAHRPAKPSIERAPTPKPGPGVLQRWENGTWVDHLL
                     DPAPLVDPVTGILKRIVARPAHPALPLGFVHWHAELPHLSSVDPHWQPDPLAPAGYLTG
                     ADPAARAIADDQPGLLSGLAHYCGAYLGQGQRHYASLTQLREAGEDALDLRDWEPHDPA
                     LHEEPGFPFIRFDIDQKLWWMAGKRGDKRCWVPLSLVHAGWMQADLPGLVAHNYHNLVG
                     LQAGNTWDEAVERACAHVVASDAVALWWAGGDRLPLAAVPPTVKQAWGECELKLTLLKV
                     PSTMKVPVVLAVVDDPAADIVTLGFAAHRDPDTAAELAVVESLIQHASAQDLNSPESLI
                     RDQGVAGLVPYDERRRYRDAFGIDLRGLIDPMCHVQRALDPRVARAVRHRCLPSRDVTP
                     APVCDSVRAALAEQHELILLDVATDRVRSAGMHVARAVVAGLARIQPAAFPLDMTARFS
                     RLARQCAWSRQHREPYPGW"
     gene            complement(6781..9018)
                     /locus_tag="JQS30_RS10140"
                     /old_locus_tag="JQS30_10130"
     CDS             complement(6781..9018)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /locus_tag="JQS30_RS10140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10130"
                     /product="YcaO-like family protein"
                     /protein_id="WP_213170170.1"
                     /sec_met_domain="YcaO (E-value: 1.2e-54, bitscore: 183.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 1.2e-73, bitscore:
                     245.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPTERTTTSLPTRPRVEIRALGSGRTLLTTPDDSSFTIDAPADL
                     VFRLIDAHVTGAASPLLPPQWYDSAEAIFSVLARGGALGEPVDERDMDDFSFDPGEEGD
                     ARAGDLLLLGDPAVIAALQGAIAQNSSVKRGVHSVSDADWQRVIHRAFRTGELLLYCVR
                     GGDDATVVAFDRLCSDARTPWVAIEVTRSTVWVGPHVRPGIGANYEDFAARRLAAAFDP
                     VAHRALRTPSVTGDCGLPPHRLGPALTAAVRALDDGLDGDVVLEINAAGEVERHPVLPL
                     PDSVISRSRPHKPTDLIDRVTGLVQRTRDVTHHRSIPAALKTAQSDVCNMRRVSRWANN
                     TSCQGSAFADVDQARASAVGEAVERYCGNLLDTQPVTFGSYKQLSRTSSMRVLDPDELV
                     LYSEAQYDAPGFPFVRFTPDLEVHWVPGHSLTLDEPVLVPASMVYVNWFTAGYSAAPVT
                     NFCPFAGIAAGHNYDYAVTSALEEIIERHATMVWWLNAQPLDAVAPDEALAALWSGVAP
                     EYGQEPSLIMLDNEFAVPVTAGVLHNRVDQLLNVGFACRDTAASAASKAWTEACTLQEG
                     SRDLLDVDGAHWDAMARGDLNSRSFKPWRADRRYLDSYRSDMRDCDDLMVQQQVLLDPR
                     AIEKVDPLLRRPVGRTLDSIPSLPERSPAAYRSALEAVGHEVIIVDLTSSDIASTGMSV
                     VRALVPGTVGNAPAAFPFLGRGVVQELAVTLGWRQRPLTESDLNYFPLPHA"
     gene            complement(9320..11122)
                     /locus_tag="JQS30_RS10145"
                     /old_locus_tag="JQS30_10135"
     CDS             complement(9320..11122)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="JQS30_RS10145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10135"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_213170171.1"
                     /transl_table=11
                     /translation="MSSDRPTSAQLLRPVLRLLEAAFADRGLFWRSTASMSAYQFAAAA
                     AAGLSTFIATIVVADPHGAQYVPLLIFALIVAVTVNGVFIWVESWLSHVLAYRILGRLR
                     MRLHDAIERISPGGMKHRRAGEVAGSTMSDVENLEWFYAHTVGAAINAALGPLLVTVAL
                     VVLTGPAGLIVLAGVAAYLAVPWLLGGLQARQGRAVRAELGELKAITLEGAEGLREILS
                     LGLDEYQKRRVLATTRRVQRRKLAFALRSGAEGALADVVVALTTVSFLLVLATRVQDNA
                     LNPLVFPAAIVLLAAAFAPATGVFAMFQRLGDMSAAAQRVLDVLDASAPVTDPAQPREP
                     TTSSGALRFADVNFGYGDTPVLRELDLDIEPGQTVALVGESGAGKTTIAHLLVRFWDPT
                     SGHILLDGVSLRDMTQEQVRQRVALIGQHPYLFRGSVRSNLHIAAPQATESELWAALED
                     AQLAPTVRSWPQGLDQLVGEGGATMSGGQRQRLAIAQAFLRSPSVLVMDEAVAHLDAAQ
                     EQQLASALARVRHNRTTVVIAHRVSTIRHADRVVFLDHGRIVATGTHDHILATCEQYRS
                     VLAHAEAPELAAQSSSEKPRGDNK"
     gene            complement(11119..12882)
                     /locus_tag="JQS30_RS10150"
                     /old_locus_tag="JQS30_10140"
     CDS             complement(11119..12882)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="JQS30_RS10150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JQS30_10140"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_213170172.1"
                     /transl_table=11
                     /translation="MIHLRLLPLARSAYAVMGLAVLVGLSLSTVAIAQALVTADIFAGI
                     ISGTPIGDLTLSLVLLSAALLARPLLVFAREMTVHLVATRVKTAIRERMLTAMFAAGPI
                     ALGRERSGTQQSHLVDGVENLEPYFGRYIPQVLITAVTSLAVVGALVLIDPVVGFVVGA
                     VAILVPFVPRLWDRLLAKRGDSHWGAYAKLNAEVVDSLRGMTTLKAFNAARRRRTQLQE
                     SSDSLLAATLHQLRISLLESGLTGLFLIAGPAIALIVGVTRVQSGAVDPLQLFAITLLA
                     FEVFRPFRELSNHWHAGYSGVSAGRRIRDLLQAGASDAPTEPTTPLTSANGGHETKAAI
                     EARSLTFTYPHAHTAALRDVDLTIDAGSTVALVGPSGSGKSTVASLLLRLAVPQHGQLL
                     IGGVPTSSLTQKQCAEIVALVSQSPVLFSGSVRENLQLVAPEADEATLKRAIDIAGAND
                     IVTDERGGLDAPVGDGGGLLSGGQKQRLAIARAVLKDAPILILDEASSALDARREESVF
                     ASLADSLTRTGGEPITTIIIAHRLASIRHVDTVFVLREGQVVERGTATELLQSDGLFAA
                     LHRAQNEQVSV"
     misc_feature    12965..12985
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.95"
                     /tool="antismash"
     misc_feature    12998..13013
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.87"
                     /tool="antismash"
     misc_feature    complement(13000..13014)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.72"
                     /tool="antismash"
     misc_feature    complement(13001..13016)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.57"
                     /tool="antismash"
     misc_feature    13027..13042
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.87"
                     /tool="antismash"
     misc_feature    13027..13045
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.82"
                     /tool="antismash"
     misc_feature    complement(13029..13043)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 26.44"
                     /tool="antismash"
     misc_feature    complement(13030..13045)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.41"
                     /tool="antismash"
ORIGIN
        1 caccatttcc ttccagtggg gcttgattgg ttagcaccaa ctgtcacaag aaatctggcg
       61 aggcgtaagt ggttacttgt ggcaacgaaa gctatgggca tttatgccca aagatgccga
      121 tgggcacaat gagctttttc aacctggaga acttgctgtt caacggtgtc tcagccccgc
      181 ttgtctactc agcgcccatc ttgcacagca gcaccttcgt aaactaccag cctcagtcgc
      241 accacacctg tggaatcggg agccaccgaa ctgtcaggat tgaaaaggct taatgaacga
      301 ggcacgttgt tcgactaaga gctcctatca ggtttgtttc cgtggcgagg atggtgtgct
      361 gtcagtgcgt ggggaggcgg aggcccttgt tcatacccag cgggtatggg caagggccga
      421 caacgcggcc aggcgcgaca tcactccacc gcagcaggaa gagaaacctg ataggagctc
      481 taagcctctg gaaattcgag acgcagcgcc acgcaagcgt gaggtgccat gtgattgcac
      541 tcgcggtgac cgctttcagc ggtatggatc tctctggcac atcgattcac caggcataag
      601 agggttggaa atgctgattg actgaggtgt tctcatcggg tttctggttt gagttgcttg
      661 aggatgttgc tggcgcggcg gggtcacagt ggaggttata tgggaggtgc caattctcta
      721 gtttcgcgaa ggtggcatag gtgtcgcctt gccgcaatca catcagagta ccccaagctt
      781 gctgacctgc atctagacgc ctatatgggg gaaccgatgg gccccgatgt ggccggctac
      841 aacgttcaag gcccgactcg acaatggcca cacagcctaa ggggccaaca acaccagttc
      901 caggtgggat ggtttgttta gtcgccaact gttctaccag gaacttgtcc catgccctac
      961 aacagcacca cacaccaagc ctccacacaa ccacctcaag ctggatggga aagcatcact
     1021 gatgcataag tttcttgttt ggtcaatggt tgggccgcaa ggcaatccac ttgcacggcc
     1081 gcatatgcac tgggcgacac ggtcgaagtg tcagtttgtt caaaacctca cctgtaacag
     1141 cagaacgttc tcacactagt ccctcattac tcacagccaa cttctacacc tacacgaccg
     1201 cccttcccca ccgaaataaa cacccccacc ctcaacccta tgctcgttac aagggtgtgc
     1261 gagcaacacc ctcttcgagg gatcaatgac agcccaaaga gggataagac cctcagtcca
     1321 agaagtgaaa tcattcctgt cgcctacgtg gccaatgata aagtaggcta tatctaatac
     1381 tgcgcgagcc gatgccccat caaacttcgc gcatcaatta ccctgcacaa catcgatcag
     1441 agcggtgaag cgcccatgac cttctcacat ttgaaggtca ccgcaaaccc tcgttggtta
     1501 gcgcccctgg agatatagcg tgcttagcac cttcggcttc gacaaagcca ccgaatcagt
     1561 ctacctagcg atgctggaga accccagcgc tggagtaacg gatctcgcct ccatcctcaa
     1621 ccaaaccgaa gagactatac gtgcctcgct cgacacattg acagagtcgg cactcgtcac
     1681 cgccgagaac gagcgcatat caccggtcga tcctgagatc ggcctggcgg ccctactatc
     1741 tcgacaacaa actcaggtct cgatgatgca gcagcaagtc gaagccagcc gcctagctat
     1801 agctcaactg ctgagcaagt acggagctaa gtcgtctgag aacaccaatg tggaaaccat
     1861 catcggtcta gccgcaatcc acgaacgcat taaggagctt tccgacgctt gccaagcgga
     1921 agctttgatt cttgccccga ccggtcgcct gcaatcaaag accgttgaaa gatccgaacc
     1981 caccgatcag gccatgctca accggggcgt ccgtatgaag accgtctacc tggacagcgt
     2041 ccgcaacgac agctacacga tggaacacat cgaaatagaa atggccgcag gcggcgaggt
     2101 ccgcacagct ccatccctac cgctgcgcat gatcatcttc gaccgtcaaa cagcactcat
     2161 ccccctcgac gacagcacat gcgaaatcgg agccctggcg ttgcgaaaca gggtcatcat
     2221 ccaggcattg gtgaccttgt tcatgaccgt atggagtgag gccaaaccgg ccaccaagca
     2281 gcgcacgcca cgttgtgatc cactgaccga ccaggagaaa cacatactac gcctctgggc
     2341 ccaaggaaac accgacgcat ctgcggcgcg caaaatgcgt gtctccattc gtaccgtccg
     2401 acgcctctcc gataagctca ctgccagact cggagctcgt agccggttcc agctgggggc
     2461 gttggccgtc tctcaatccc ttatcggcga agcagatatt gtattatacg cccattttgg
     2521 gcttaattgc cgaatggagc ttttcactaa aaacgggcat aactatgtgg gggttcgggc
     2581 atctgcccga acccccacat taatgctttg tcgtaggttt actaatcgtc ccacccggtt
     2641 ccattcgtgt gcgcggcagc cagatatggt tccaccgcta cttcctcacc ttgctctatc
     2701 gcgccgttga tagacgcggc cgagaagaac aaaaatgcta gggcaacagc ggtacgtcgc
     2761 attcaggaga tcctttcata aggggtgcct actaccccca aagctagaca ccttccagcc
     2821 actccgaaag aggtcaaaag tgctcggccc gcacgtccgc aaaagtaacc taggaaccct
     2881 aatttttgtc aataaaggga aatatagctc attcagattc atttggaagc gcatacatag
     2941 agccaatgag cccggacaac accaatggaa acactcggcg aaaagtttca tatatagatt
     3001 tgacatcgct ttggtcacca tctatgaaaa tctcctacca cgcagcaaat aagtatatgc
     3061 accaatgcaa tatattctgc tttcataaga gctcctatca ggtttgtttc cgtggcgagg
     3121 atggtgtgct gtcagtgcgt ggggaggcgg aggcccttgt tcatacccag cgggtatggg
     3181 caagggccga caacgcggcc aggcgcgaca tcactccacc gcagtaggaa gagaaacctg
     3241 ataggagctc taagggcacg tatgcaaaaa tgcaagctca aggccacagg agacgaagtt
     3301 tacgcaggta aacgagccgg cgagaatgcc gaaagcttcc tttacataag cttcaagtgt
     3361 gctcgcctat gcatttaggc gagcacacac gacaaaaagg ccgaggctaa tgcctcggcc
     3421 ttgaactgga gtatcacatc tccagggccc agcacgtgta atcgtcgagt ggggccactg
     3481 cacggcgtat tagctgttca atacggacct gagcctcttg gtgcccttct actccggtgt
     3541 aaaccacgac ctccacgcgc tccgaaaccc gcacgtaaac atgctcgact agatcctgcg
     3601 gagattgcgc cgacaacacg cttcgtttta tgcaatcggg ttcaagatct tgaaacgcac
     3661 ccttgagtag acaaacgatc atctgcatgg gattggtttc cgtcgtatca gatcgacaag
     3721 tcggcggcta ctcttggaca ccacgggact cctgttgtca cggcaattac aaagaacatc
     3781 tgtataaact ttctaaacca cttagctgtt acggttaggt catgaccaaa taatacggtt
     3841 tgtccccgct tcacgtcagt ggtcataagc tgccctgcca cagtcgaaca gttgtgacct
     3901 ccccaggccc gacggagaat cgacctcctg cgcggctact aggtgttcag cctgcacgct
     3961 ccagaatatg ccgtgcccgc ccattggcga ccgctactga tgccaatcgc gacaaccaac
     4021 gatgcgttgg cataaacgcg aaccctgctt ccatagcctt atgcggcgac attgtgaagt
     4081 cactcggata ggagaacggc gaacgcctct ctgcatcgga gagctgatcg cgttcttcga
     4141 tgatcgcctt cactccagaa acgtcctcaa tgacagcgca catatcggcc acactcaccg
     4201 gcttcacaga gcacacgttg tatgtcccct caacagtgga cgtggccgcc caggccagaa
     4261 accgggccac gtctagagct agaacaactg aggtcaaccc cgcatcaaca tgggtgagaa
     4321 tcggctgtcg cgcgagcacg cggtcaacat ggaactggaa acgacccgtg aagtccccaa
     4381 cttcggcgaa cacgtgcgcg gcccggggaa tcaccaccgg taccggtgcg ttctcccata
     4441 gagcggcctc ggcctggcgc ttaccctccc cgtaattttg ggcaatgtag gcctgttcgt
     4501 gccagggcag ttcggtgtcg taaccgtagg ttcgcgtatc gagttcctcc tccaacgcga
     4561 agcccccgat ctgcggcgcg gcctggccaa acgaggaggg attgtatacc tcgatcgtgg
     4621 aggtcatgac atacttgccc acccgatcgc cgaaggccgc cacggcggcc aatgccgcat
     4681 tcggtgtgta gcagacctgg tggatcaccg cgtcgaagga gcgtccggca acagcggagg
     4741 tcagttgctc ggcgtcggtg gcgtcggcgt ggaggcgtcg cactgagtca cccaggtcat
     4801 cggcgctttg tcctctggtg atgatcgtga cgtcatgacc ttcgtcgatg agagtttgca
     4861 ccaggagctt gccgacgaat ctcgttccgc caatgacgca tacgtgggcc acaagagtgt
     4921 cctttcgggt gtttgcgttg aactctgcgg acgcaacagg ggtgggaccg cagtcagtct
     4981 aactgacggt aaagcctgtt tcaccaaccc ggatatggct cccgatgttg tcgggaccag
     5041 gcacattgac gcgctagacg actaaaacgg gcggtcatat ccaaagggaa agcggcgggt
     5101 tgtatacggg ctaggccggc aaccacggcg cgggcaacgt gcattcccgc agagcggacg
     5161 cggtcggtgg ccacgtcaag caggatcagc tcgtgttgtt ctgccaatgc agcgcgcaca
     5221 ctgtcgcaaa cgggtgctgg ggtcacgtcg cgtgagggga gacagcggtg gcggacggcc
     5281 cgggcaaccc ggggatcaag cgcccgctgc acgtggcaca tggggtcgat gaggccgcgc
     5341 agatcgatgc cgaaggcatc gcggtagcgg cggcgctcat catagggcac cagcccagcc
     5401 acgccctggt ctctgatcag actttcgggt gaattgaggt cctgggccga ggcatgttgg
     5461 atgagcgatt cgaccacggc tagttcggcg gcagtatcgg ggtcgcggtg agcggcgaaa
     5521 ccaagcgtca cgatgtcggc ggcaggatca tcgaccacag ccaagacaac tgggactttc
     5581 atagtggagg gaactttcag cagcgttagc ttcagttcgc actctcccca agcctgcttg
     5641 accgtcggtg ggacggctgc cagcggcaat cgatcaccac cagcccacca cagggccacc
     5701 gcgtcgctgg ccaccacgtg ggcacaagcg cgctccacgg cctcatccca agtgttgccg
     5761 gcttgaaggc cgacaaggtt gtgatagtta tgcgccacca ggcccggcaa atcagcttgc
     5821 atccaaccgg cgtggacgag gctgagggga acccagcaac gtttgtctcc tcgttttccc
     5881 gccatccacc acagcttttg gtcaatatcg aagcggatga aggggaagcc gggttcctcg
     5941 tgcagggctg ggtcatgcgg ttcccaatca cgtaggtcaa gggcatcttc tccggcctct
     6001 cgcaactgcg ttagagaggc atagtggcgt tggccttggc ccaggtaggc cccgcagtag
     6061 tgggccaagc ccgacagcag gccaggttgg tcatccgcaa tggctctggc agcggggtcg
     6121 gccccggtga ggtatccggc cggagcgagc ggatcgggtt gccagtgtgg gtcaacgctg
     6181 ctcaggtggg gtagttcggc gtgccagtgc acgaatccca gtggcagagc cgggtgcgct
     6241 ggccgagcca cgatgcgttt gaggatgccc gtcactggat cgaccagcgg cgctggatct
     6301 agaaggtggt cgacccaggt gccattttcc caccgttgca aaacccctgg tccaggcttg
     6361 ggagtcggtg cacgctcgat tgatggtttc gccgggcggt gggctggccc agccaacacc
     6421 gccgccaagt ccgcatcggg gatcgtcgcc cgaagacagt caacggcagt gcgcaatgcc
     6481 gcgtggtcgc gtcgcatccg gtaccagtgt ccggcgaaga accacccggt gacgtcctct
     6541 gcgcggcgtc cgccagtagt cgcgcggtga acgccaccga gcagttcatc tgtgctggag
     6601 accagcacgt cgtgggggtc gaaccgtatc ctcacggcgg gcccggctag tgcggccagg
     6661 tgggccagga ctgttgagga gagcacatcg tcaggggagg cgtcgatgcg ccattgccgt
     6721 ccaagtgggg cgaccaccaa cgattcggtt ccgaccgcgt atatggcgag ggaatctacc
     6781 tcaggcatgt ggaagcggga aatagttgag atccgactcg gtcaagggcc tctgacgcca
     6841 gccaagagtg accgccagct cctgcacaac gccacggccg aggaagggga aggccgccgg
     6901 agcgttgccg acggtgccgg gcaccaacgc acgcaccacc gacatgccag tgctggcgat
     6961 gtcagacgac gttaggtcca caatgatcac ctcgtgtccg accgcctcga gcgcacttcg
     7021 atatgcggcc gggctgcgtt caggcagcga cggtatggaa tccaaggtgc gcccgaccgg
     7081 tcggcgcaga agtggatcga ccttctcaat cgcccggggg tcgagcaaca cctgttgctg
     7141 caccatgagg tcgtcgcaat cgcgcatgtc gctgcgatag gaatcgaggt atcgccggtc
     7201 ggcccgccag ggtttgaacg aacggctgtt gaggtcaccg cgagccatcg cgtcccagtg
     7261 cgctccatct acgtcgagga ggtcgcggct tccctcttgc aatgtgcagg cctcggtcca
     7321 ggctttggag gcggccgagg ccgcggtgtc gcggcaggcg aacccaacgt tgagcagctg
     7381 gtcaaccctg ttgtgcagga caccggccgt caccggcacc gcgaactcgt tgtcgagcat
     7441 gatgagcgaa ggctcttgcc cgtactcggg agcgaccccg ctccacagtg ccgcgagtgc
     7501 ctcatcgggg gccacggcgt ccagtggttg cgcgttgagc caccacacca tggtcgcgtg
     7561 ccgttcgatg atctcttcga gtgccgaggt taccgcgtag tcatagttgt gtccggccgc
     7621 gatcccagcg aagggacaga aattggtcac cggcgcggcg gagtatccgg cggtgaacca
     7681 attgacatag accatgctgg cgggcactag caccggttcg tccagagtga gcgaatgccc
     7741 cggtacccag tggacttcca gatcaggggt gaaccgcaca aacggaaatc ccggggcgtc
     7801 gtattgcgcc tcggagtaca agaccagttc atcggggtct agcacccgca tggagctggt
     7861 gcgagacagc tgtttatagg acccgaaggt gaccggttgg gtgtccaaaa ggttgccgca
     7921 gtatcgttcc acagcttcgc ctacagcgct ggcccgtgcc tggtcgacat cggcgaacgc
     7981 ggagccttgg cacgaggtgt tgttggccca tcgcgatacc cgacgcatgt tgcacacgtc
     8041 gctttgcgct gtcttgagcg ctgcggggat gctccggtgg tgcgtgacgt ccctagtgcg
     8101 ttgcactagt ccggtcacgc ggtcgatcag gtcggtcggt ttatgcggtc gcgaccgcga
     8161 aataacacta tctggcagtg gcaacaccgg atgccgttcc acctcacctg cggcgttgat
     8221 ttcgaggaca acgtcgccgt ccaatccatc gtccaaagct ctcaccgctg cggtgagagc
     8281 cgggcccaac cggtgaggcg gtagcccaca gtcgccagtg accgatggag tacgcaatgc
     8341 tcgatgagca accgggtcga aggcggcggc aagtcggcga gcagcgaagt cttcgtaatt
     8401 ggccccaatg ccagggcgta cgtgcgggcc gacccataca gtcgaacggg tgacttcgat
     8461 cgctacccag ggggtgcgag catccgaaca gaggcggtcg aaagccacca ctgtggcgtc
     8521 atcgccccca cgcacgcaat agaggagcaa ctcgccggtc cgaaaggctc ggtggatgac
     8581 gcgctgccag tctgcgtcgg aaacgctatg gacaccccgt ttgaccgagg agttctgcgc
     8641 gatggcacct tgcaacgcgg caatcacggc cgggtcgccc aacagcaaca ggtcgccagc
     8701 gcgggcgtcg ccttcctcac cggggtcaaa ggagaagtca tccatgtcgc gttcgtcgac
     8761 gggctcccct agcgcaccac cgcgggctag gacgctgaag atcgcctcgg cgctgtcgta
     8821 ccactgaggt ggcagcagcg gtgatgccgc gcctgtgaca tgagcgtcga tgagccgaaa
     8881 taccaggtca gctggtgcgt cgatggtgaa cgaactgtca tcgggtgtcg ttaggagcgt
     8941 gcgaccggag ccgagggcac ggatctcgac gcgagggcgg gtggggagcg aggtggtggt
     9001 tctctcggtc ggcgacatgg tttctcctgg ggtggaagga atcgacggct gggtggcggg
     9061 tgcctcgctg accaagacac ccgcccttac gtgtgggccg tatcgatgcg cttccactgg
     9121 ggtgggatcg acattgtcaa tccggcgacc ccatggggct catgtcaacg accagtgggt
     9181 taggaccagg cgaaagcggt cgagacgtgg ccgtggttgt tcgcgttggg ggccaggatc
     9241 tggttctcgg agcgctccgt ggccacctcg gtgacggtga tcttcttttg catctttcat
     9301 ctcctctcaa ggggtctttt catttgttat cgccacgcgg tttctccgat gaggactgcg
     9361 cggcgagttc gggggcctcc gcatgggcga gcacggaccg gtactgctca caggtggcca
     9421 ggatgtggtc gtgggtgccg gtggcgacga tgcggccgtg gtcgaggaaa acgacccggt
     9481 cggcgtggcg gatggtggag acgcggtggg cgatcacgac ggtcgttcgg ttgtggcgta
     9541 cccgggccaa ggcgctggcg agttgttgtt cttgggcggc gtccaggtgg gcgacggcct
     9601 cgtccattac caggacgctg ggtgaacgta gaaatgcttg ggcgatggcg aggcgctggc
     9661 gttgtccgcc cgacatggtc gccccgcctt ctccgactag ctggtcgagc ccctgtggcc
     9721 aagagcgaac ggtcggggcc agttgggcgt cttccagggc cgcccatagc tcggattcgg
     9781 tcgcctgggg cgcggcgatg tgtaggttgg atcgaacact gccacggaag aggtaggggt
     9841 gttggccaat gagggcgacg cgttggcgaa cctgttcttg cgtcatgtca cgcagggaga
     9901 caccatcgag caggatatgt ccggaggtgg ggtcccaaaa gcgcacgagt aggtgcgcga
     9961 tggtggtctt tcccgccccg gactcaccga cgagggccac ggtctgtccg ggctcgatat
    10021 ccaggtccag ttcacgcagc acaggcgtgt cgccatagcc gaagttgaca tcggcgaacc
    10081 gtagggctcc gcttgaagtg gtgggttcgc gcggctgagc ggggtcggtc actggtgcgg
    10141 aggcgtctag gacgtcgagt actcgctgcg cggccgccga catgtccccc aggcgttgga
    10201 acatcgcgaa tactccggtc gcgggagcga aggcggcggc taggaggacg atcgcggcgg
    10261 ggaacaccag tgggtttagc gcgttgtctt gcacgcgggt ggccaagacg agcaggaagc
    10321 tgacggtggt gagggcgaca accacgtcgg ctagtgctcc ttcggctccg gatcgcagcg
    10381 caaaggcgag tttgcgccgt tgtactcggc gggttgtggc gagtactcgc cgcttctggt
    10441 attcgtcgag gcccagggag agtatttccc gcagcccttc ggcaccttcc agtgtgatcg
    10501 ctttgagttc gcctagttcg gcgcgcacgg ctctgccttg gcgagcttgc agtccgccca
    10561 gcagccaggg tacggccagg taggcggcta ctccggccaa gacgatgagc ccggctggtc
    10621 ccgtgagcac gacgagggcg acggtgacca agagcggccc gagggcggcg ttgatggccg
    10681 cgccgacggt gtgggcgtaa aaccactcca ggttctccac atcggacatg gtggagcccg
    10741 cgacttctcc ggctcgacgg tgcttcatac cgccggggct gatcctttcg atggcatcgt
    10801 gcagacgcat ccgcaatcgg ccgaggatgc ggtaggcgag cacatggctg agccaggatt
    10861 cgacccagat gaacactccg ttgactgtga cggccactat tagggcaaag atcagcagcg
    10921 gcacgtattg tgccccgtgt ggatcggcta cgacaatggt ggcgataaac gtgcttagtc
    10981 cagcagcggc cgcggcggcg aactggtagg ccgacatact ggccgtggaa cgccagaaca
    11041 ggccccggtc ggcaaaggcg gcctccagta gtcgcaggac cggtcgcaga agttgcgcgc
    11101 tcgtggggcg gtcgctactc atacactcac ctgttcgttt tgggcgcggt gcaaggcggc
    11161 gaagagcccg tcgctttgca gcagttccgt ggcggttccg cgttccacca cctgtccctc
    11221 tcgcagaacg aacacagtgt cgacgtgccg gatcgaggca agccggtgcg cgatgatgat
    11281 agtggtgatg ggctcgccgc cggtacgagt cagggagtcg gccaaggagg cgaagacgga
    11341 ttcctcccgg cgggcgtcta gtgcgctcga ggcctcatcg aggatgagga tcggcgcgtc
    11401 tttgaggacg gcccgggcga tggcgaggcg ttgtttttgc ccgcccgaca gcagccctcc
    11461 gccatcgccc actggggcat ctagcccgcc tcgttcgtcg gtcacgatgt cgttcgcgcc
    11521 agcgatgtca atggctcgct ttagagttgc ctcgtcggcc tcgggggcga ccagctggag
    11581 gttttcgcgc acgcttccgg agaaaagtac tggtgactgt gacaccagcg ccacgatttc
    11641 ggcgcattgt ttctgggtga ggctggaagt ggggactccg ccgatgagca gctggccgtg
    11701 ttggggcact gccaggcgca gcaacaggct ggccaccgtt gatttgcccg atccggatgg
    11761 gccgaccagg gccaccgtgc tgcctgcgtc gattgtcaag tcaacgtcgc gaagcgctgc
    11821 tgtgtgcgcg tgtgggtagg tgaaggtcag gctgcgcgct tcgatggcgg ctttggtttc
    11881 gtggcccccg ttggccgaag tcaggggggt ggtcggctcg gtgggcgcgt cgctggcccc
    11941 ggcttggagt aggtctctga tgcggcgtcc agcggagacg ccgctgtatc cggcgtgcca
    12001 gtggttggat agttcgcgga agggtcggaa gacttcgaag gccagcaggg tgatcgcgaa
    12061 caactgcaac ggatcgacgg ctccgctttg cactcgtgtg acgccgacga tgagggcgat
    12121 cgcgggtccg gcgatgagga acagtccggt gaggcccgat tccagcagcg agatgcgcag
    12181 ttggtgcagg gtcgcggcaa gcaggctgtc gctcgactct tgcagttgcg ttcgtcgacg
    12241 gcgggccgcg ttgaatgctt tgagggtcgt catgccacgt agggagtcca ctacttcggc
    12301 attgagtttg gcgtatgccc cccagtggga gtcaccgcgt ttggcgagga ggcggtccca
    12361 taggcgggga acgaagggca ccagtatcgc cacggctccg acgacgaacc ccaccactgg
    12421 gtcgatgagg accagtgcgc ccaccacagc cagggatgtc acggctgtga tgaggacttg
    12481 ggggatgtat cgcccgaagt acggttctag gttttcgact ccatcgacta gatgtgactg
    12541 ttgggttccg ctgcgttctc ggcctagggc gatcggtcca gcggcgaaca ttgcagtgag
    12601 catccgttcg cggatggcgg ttttgacgcg ggtggcgacc aggtgcacgg tcatttcgcg
    12661 ggcgaagacc aagagcggcc gggccaggag ggcggcgctg aggagcacta gcgacagcgt
    12721 caggtcgcct atgggggtgc cggagatgat gccagcgaag atgtcggcgg tgactagggc
    12781 ctgggcaatg gccacggtgc tgagcgatag accgacgagg acggccagcc ccatgaccgc
    12841 ataggcgctg cgggccaggg gaagtaggcg cagatgaatc acgtcgactc gctttcgatc
    12901 accgggtttg gtctctaggg caccggctgt gccggggctt ggggtgcggt cgcactcgcg
    12961 acccttttcg tcacacaggc gcgtacaccg cccacttaat gaagatgatt atcgttatgc
    13021 tagcttattg aaaatgattg tcaataacgg ggtggaagaa aattcttcct cgcctggccg
    13081 cccccaccac tgcgccacgg cctccccagc acaacgcaca gccacgcatt gaaggcggac
    13141 cagactccat gacagccaca gtcgcgcgcc ccacgcccca agcggcacag aaaaagaccc
    13201 cctcgcaaac gccaggacaa cgccaacacc ggcgcgccat cgtctggacc gtggccctgg
    13261 ccggcgccct cgtcgtctcc gccatcgtca ccatcgggct cggaccggtc cccatcccgc
    13321 cagacgtagt cgcccgcatc ctcgcccacc acctcttcgg aatcggggaa gtgacctgga
    13381 cagcctcgga cgacaacatc gtgtggctct tgcgaatccc ccgagtccta ctgggaatct
    13441 gcgtcggcgc gatcctctcc atcgccggag tcgctgtgca atcactcgta cgcaacccac
    13501 tagccgaccc ctacctcctg ggcatctcct caggtgcctc cgccggagcc gccgccagca
    13561 tcctcttcgg cgtcggcgcc ggagcactcg cgctcaccgg cagcgcattc ctcggcgccg
    13621 tcgccgccat cgtcctcgtc ttcgccgcct gccgcatggg cggacaactc atccccgccc
    13681 gcctagtctt cgccggcatc gccgtcggct tcgccctcac cgccgtcacc aacttcttgg
    13741 tcttcaccag cgactcccga gatggagccc gggccgtcat gttctggatg ctcgggtccc
    13801 taagccaagc gcgctggcaa tccgtaccca tcgccgccgc cgcgctcctc atcgccctag
    13861 ccaccctgct gtggtgggcc cgccgactcg acgccatcgc catcggcgac gacgcggccc
    13921 gcagcctcgg caccaacccc tcaaccttcc gcgccggatg cgccctgctg gtgtgcttcg
    13981 ccgtcgccgc cgccgtctcc gtctccggcg caatcggc
//
