LOCUS       NZ_CP070260            10945 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces californicus strain FDAARGOS_1210 chromosome.
ACCESSION   NZ_CP070260
VERSION     NZ_CP070260.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces californicus
  ORGANISM  Streptomyces californicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 624.55x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016906265.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:02:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,147
            Genes (coding)                    :: 7,032
            CDSs (with protein)               :: 7,032
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 36 of 115
            Pseudo Genes (incomplete)         :: 97 of 115
            Pseudo Genes (internal stop)      :: 12 of 115
            Pseudo Genes (multiple problems)  :: 27 of 115
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:25:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7832621
            Orig. end                         :: 7843566
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="32"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5945
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(472..485)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 21.12"
                     /tool="antismash"
     gene            716..1504
                     /locus_tag="I6J40_RS33935"
                     /old_locus_tag="I6J40_33925"
     CDS             716..1504
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060653.1"
                     /locus_tag="I6J40_RS33935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33925"
                     /product="VOC family protein"
                     /protein_id="WP_030116997.1"
                     /transl_table=11
                     /translation="MTASERTTPVHGTPCWVSLMTRDLRAAQDFYGAVLGWTFRSGSLG
                     EGFSVAHLGALPVAGLGQIAPGLPTTVSWTPYFAVADVDTAAARVRERGGTIAVGPIRL
                     GPGRGAVAADREGAGFGFWEGRTPPWSLGRDDAPASLELRTSHAFDSAIFYAEVFGWAS
                     REPGGCDVGYACLQDAVVVRQGGRTVATLRGGGDASSADPRLRPRWHVRFRVADAEAAG
                     EAARAAGGSAGPSVDSPADGTEAVIRDPEGGIFTVCAHPG"
     gene            complement(1526..2386)
                     /locus_tag="I6J40_RS33940"
                     /old_locus_tag="I6J40_33930"
     CDS             complement(1526..2386)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971205.1"
                     /locus_tag="I6J40_RS33940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33930"
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="WP_030190240.1"
                     /transl_table=11
                     /translation="MPHHAARPLLRRRRTLAALACGIAVATGAGVLLSHASATGTTAGA
                     VPEVVPPTAHVRFDYQLGGAYPPPAGVGAVSRDRGARPADGLYNVCYVNAFQTQPDALD
                     WWETNEPDLLLRDGDGEPVEDDAWGEALLDTSTATKRSRLAKIVGGWIDGCATSGFQAV
                     EPDNLDSYERSDGLLTPEHNAAFAKLLADRAHAAGLAIGQKNTTDLLERRKEIGFDFAV
                     VEECGRYDECGDFADAYDDRVFVVEYADRDFARACASVGTRASVVRRDLDVVPAGRPGY
                     VHRTC"
     gene            complement(2425..3762)
                     /locus_tag="I6J40_RS33945"
                     /old_locus_tag="I6J40_33935"
     CDS             complement(2425..3762)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060651.1"
                     /locus_tag="I6J40_RS33945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33935"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_030190242.1"
                     /transl_table=11
                     /translation="MAGPVTRRSPARRRLRRLQLRLTAAYTLITVVGLACLSWLVIRTD
                     DRARQDAEYDEMRRRASVAASLVYYEDGRIRLDGLHDDEATAGTPQILVLEGRPGADPV
                     TVFRGRAAPFAVPAGPVAAVARAAMADEGPARADARDRSGRPVRLLGVPFHHDATDEVA
                     GAAVAVGDPERGADEHRSLVLSLVVGAGALTALAALTGHVLSGRSMRPAWEALEQQERL
                     LADAAHELRTPVAVMRGSVELAAGAPGGLDAHLPRIRRAAERMSDVVENLLTRGRLEAA
                     VDAVRAEPLRLDQLVEEVCAELPESAPRPRLRLAESVVEADAALVRVAVRNLLDNAVRH
                     GRAAEAPEGDGLLVTVRGPEVAVADRGPGVDPARLPELTERFRSPGGGTGIGLSLVRRV
                     AEAHRGMLAVRPRPGGGAEFVLRLAPARPRRGSRGGPHDSLMIGRS"
     gene            complement(3762..4436)
                     /locus_tag="I6J40_RS33950"
                     /old_locus_tag="I6J40_33940"
     CDS             complement(3762..4436)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="I6J40_RS33950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33940"
                     /product="response regulator transcription factor"
                     /protein_id="WP_030117000.1"
                     /transl_table=11
                     /translation="MRVLLVEDDEDLREMTAAGLRDGGFAVDCAADWPEADVLLHVNAY
                     DCVVLDRMVPSGDTLAPLEAGRRAGWSVPVLCLTALDSLDERVRGLESGADDYLAKPFA
                     MRELVLRVRGLSRRAPVRLPSFLRCADVVMDVARHEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTVLLEHCWDEMADPVSNVVDAVIAGLRRKLGSPALVHTVRGQGFLLSARTE
                     PS"
     misc_feature    4728..4743
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.87"
                     /tool="antismash"
     misc_feature    complement(4733..4747)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.23"
                     /tool="antismash"
     misc_feature    complement(4734..4747)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.06"
                     /tool="antismash"
     misc_feature    4734..4748
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.52"
                     /tool="antismash"
     misc_feature    complement(4735..4748)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    4735..4749
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.82"
                     /tool="antismash"
     misc_feature    4910..4923
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.2"
                     /tool="antismash"
     gene            5001..5945
                     /locus_tag="I6J40_RS33955"
                     /old_locus_tag="I6J40_33945"
     CDS             5001..5945
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="I6J40_RS33955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33945"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_030190244.1"
                     /sec_met_domain="AfsA (E-value: 6.1e-24, bitscore: 82.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPVRAMDTEADVTHPVGIDMVHRTRSQDAFPRGWMRL
                     EHDRFSVTAVLPHDHAFFAPLAGDRHDPLLVAEAMRQAAMLAFHAGYGVPMGHHFLLSG
                     LDFVCHLEHLGVGGGPAEIGVEVYCSDLRWRAGLPVAGRVTWAVHRDGGLAATGVGVTR
                     FISPEVYRRMRGDAPAEGIAIPRAAPVRADLAGRARTEDVVLSETDRDGWWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAACLAAGPGGIVPAEVASRFHRYAEFDSPCLIGAVVR
                     PGPAEGTVTARVSGRQNGELVFTAVLTGPRVHG"
     gene            5961..6818
                     /locus_tag="I6J40_RS33960"
                     /old_locus_tag="I6J40_33950"
     CDS             5961..6818
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972658.1"
                     /locus_tag="I6J40_RS33960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33950"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_030190246.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAAQLAAAGPVRILARRPERLGAFGNRAEVV
                     AGAYADRQALGRALRGVTSVFLVTNSPTEPDDERVAAAAAEAGVRHLVKLSMMAVEEPD
                     ASDLITRLQRENERTIRESGVPWTFVRPRTFMSNTLSWAAGIRSDGVVRALHGDAPVAC
                     VDPRDVAAVAVAALTGTGHEGRAYAVSGPEAITAREQTAQLSRALGRPLRFEELGVAAA
                     RTALLAKYPRPVAEAFLESAERQRAGAKAGVVSTVRQLTGRSARPYNVWSADHADAFTG
                     RDDR"
     misc_feature    complement(6899..6914)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.96"
                     /tool="antismash"
     gene            7129..7827
                     /locus_tag="I6J40_RS33965"
                     /old_locus_tag="I6J40_33955"
     CDS             7129..7827
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032569.1"
                     /locus_tag="I6J40_RS33965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33955"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_078866655.1"
                     /transl_table=11
                     /translation="MTKQERAARTRQALIRSAAAVFEQHGYAQARLAQISSGAGVSTGA
                     LHFHFDNKAAVAQAVVAEASRRLRELSAAVRRGTDNALQALVDTSHALLERLRDDPVSR
                     AGFRLSCDGDEAAVPDLRLEWHERVRELLDEAAATGALAEGVSREDTAAATVAVTAGFE
                     ALGRNDSAWLAPPMLTGFWNLLLPRIAAAETVAHVDPSGARAAEPALRVPGPALARPAR
                     GAVEPAAGVN"
     misc_feature    7678..7680
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7856..8785)
                     /locus_tag="I6J40_RS33970"
                     /old_locus_tag="I6J40_33960"
     CDS             complement(7856..8785)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="I6J40_RS33970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33960"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_032745765.1"
                     /transl_table=11
                     /translation="MDDQPEAASPEPLDRRAELSEFLRTRRAKLQPQDVGLPEFGRHRR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLTHLARP
                     ARHKKKRRPARAQRVRTGLLYLLESMEGVPAYVTGARSDILAWNPMAAAVFGDWGALPP
                     GERNWARLVFLSPAYRDLFVNWDSKASDMVSYLRLYAGCHPDDPELSALVGELSVKSEE
                     FRRLWATHNVKEKGHGTKLVRHPLAGDLTLSYETLNLPDDEEQHLVTYHAEPGSASAEG
                     LRLLASWGADAGRRDLFDDAGRGPAGGR"
     misc_feature    8793..8807
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.66"
                     /tool="antismash"
     gene            8948..9988
                     /locus_tag="I6J40_RS33975"
                     /old_locus_tag="I6J40_33965"
     CDS             8948..9988
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-268). E-value: 8.5e-13.
                     Score: 40.0. Matches aSDomain:
                     nrpspksdomains_I6J40_RS33975_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="I6J40_RS33975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33965"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_030190249.1"
                     /sec_met_domain="ADH_N (E-value: 6.5e-29, bitscore: 98.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.9e-20, bitscore:
                     71.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGPGVTAFKVGDRVGVGCMVDSCGQCDACRAGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHIVVRDAFVVSIPEGIALDEAAPLLCAGITT
                     YSPLKRWGVGPGKKVAVVGLGGLGHMAVKIAHTLGAEVTVLSQTLRKKDDGLKLGADHY
                     YATGDPATFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPISLNLFSL
                     IGGDKAIAGSMIGGIGETQEMLDFCAAHGLGAEIEVIEASRVNEAYERVLASDVRYRFV
                     IDAATI"
     aSDomain        9005..9751
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS33975_PKS_ER.1"
                     /evalue="8.50E-13"
                     /label="I6J40_RS33975_PKS_ER.1"
                     /locus_tag="I6J40_RS33975"
                     /protein_end="268"
                     /protein_start="19"
                     /score="40.0"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGPGVTAFKVGDRVGVGCMVDSCGQCDACRAGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHIVVRDAFVVSIPEGIALDEAAPLLCAGITTYSPLKRWGVGPGKKVAVVG
                     LGGLGHMAVKIAHTLGAEVTVLSQTLRKKDDGLKLGADHYYATGDPATFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVG"
     gene            complement(10095..10472)
                     /locus_tag="I6J40_RS33980"
                     /old_locus_tag="I6J40_33970"
     CDS             complement(10095..10472)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515163.1"
                     /locus_tag="I6J40_RS33980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_33970"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_199807297.1"
                     /transl_table=11
                     /translation="MSTPELSTPERAAASGRRIAHPRPEEIRLEAVLHALADPVRLRIA
                     RELANGHEDMACIAFDLPVSKSTSTHHFKVLREAGVIRQHYDGTSRMSRLREDDLEALF
                     PGLLAAVLEGARRSAAPPDPA"
     misc_feature    10512..10528
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.33"
                     /tool="antismash"
     misc_feature    complement(10535..10550)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.15"
                     /tool="antismash"
ORIGIN
        1 ggtccgtgat cacgtgcacc aggatctggt gcagggtcac ccgcgcccgc tcctcgggcc
       61 accaccgcac ccggcccacc gcgtcgaggg gcaggtcggc gatggtggcg tccgcgtgcc
      121 gcgcgatccg gcggtagagg tcgatgacgt cctcgcgcga ctcgtcggcc ctcgcccaca
      181 tgtcctcgtt ggcctcggcg tcgtccgaga acgcgtgcgg cgtcccggtg aacggccggt
      241 cgaacgtctc gccgaaatac ccgagctcca cccccgccgc gtgcttgacc agcccgagga
      301 ggttggtccc ggtcggcgtc agagggcggc ggacgtcgta ctcggaaagc ccttcgagct
      361 tccacagcag agtgtcgcgg gcggaccgca gatagcgctg gagatcagcc ttcggctgcg
      421 cggggttcat acggggaagt ctgccagcgg ccactgacac cggccgcggc gtcacggagc
      481 gtggccgccg gggccgcccg ggtccggccg accgcgtacc cggccgttcg ggcgcgccgc
      541 ccgggtggcg gcggcgaccc gggcggcgta ggtatggagg cgtgccgttc ccgacggcgt
      601 gcgacgcccc ggagcgcccc gatccggtga cggccaatca ccaccagacc cacccgccgg
      661 gccgtggccg ggccccccgg cggccgacct gtgggaacga ggagacggat tcccgatgac
      721 cgcgtcagag cggaccaccc cggtgcacgg cactccgtgc tgggtcagcc tcatgacccg
      781 ggacctgcgg gcggcccagg acttctacgg ggccgttctc gggtggacct tccgctccgg
      841 ctcgctcggc gaggggttct ccgtggccca cctcggcgcc ctgcccgtcg ccgggctcgg
      901 gcagatcgcc cccggcctgc ccaccaccgt ctcctggacc ccctacttcg cggtcgcgga
      961 cgtcgacacc gccgccgccc gggtccgcga acggggcggc acgatcgccg tcggcccgat
     1021 ccggctgggc cccggccggg gagcggtggc cgccgaccgg gaaggagccg gattcggctt
     1081 ctgggaggga cgcacgccac cctggtcgct cggccgcgac gacgccccgg cctccctcga
     1141 actgcggacg agccacgcct tcgactccgc gatcttctac gccgaggtgt tcggctgggc
     1201 ctcccgggag cccggtggat gcgacgtcgg ctacgcctgc ctccaggacg cggtcgtggt
     1261 ccgccagggc ggccgcactg tggccaccct gcgaggcggc ggtgacgcct cgtccgccga
     1321 cccccggctg cggccgcgct ggcacgtccg tttccgggtc gcggacgcgg aagcggccgg
     1381 cgaggcggcc cgagcggccg gcggctccgc cggcccgtcg gtggactcgc ccgccgacgg
     1441 gaccgaagcg gtgatcaggg accccgaagg cggcatcttc acggtctgcg cccatccggg
     1501 atagcgccac gccccgcgac ggggctcagc aggtgcggtg gacgtacccg ggacgcccgg
     1561 ccggcaccac gtcgaggtcc cggcggacca cggacgcccg cgtccccacg gacgcgcacg
     1621 cccgcgcgaa atcgcggtcg gcgtactcga cgacgaacac ccggtcgtcg tacgcgtcgg
     1681 cgaagtcgcc gcactcgtcg tatcggccgc actcctccac gacggcgaag tcgaacccga
     1741 tctccttccg ccgctccagc agatcggtgg tgttcttctg gccgatcgcc agcccggccg
     1801 cgtgcgcgcg gtcggccagc agcttcgcga acgccgcgtt gtgctccggg gtcagcaggc
     1861 cgtcggaccg ctcgtaggag tccaggttgt ccggctccac cgcctggaaa ccggacgtgg
     1921 cgcagccgtc gatccagccg ccgacgatct tcgcgagccg ggagcgcttg gtcgcggtgg
     1981 aggtgtcgag gagcgcctcc ccccacgcgt cgtcctcgac cggctcgccg tcgccgtcgc
     2041 gcagcagcag gtcgggctcg ttcgtctccc accagtccag ggcgtcgggc tgggtctgga
     2101 acgcgttgac gtagcagacg ttgtagaggc cgtcggccgg cctggcgccg cggtcgcggg
     2161 agacggcccc gaccccggcg ggcgggggat aggcgccacc gagctggtag tcgaagcgca
     2221 catgcgccgt gggcggcacg acctcgggca ccgccccggc cgtggtcccc gtggcggacg
     2281 cgtgggacag gagcacaccc gctcccgtcg cgaccgcgat tccgcaggcg agggcggcga
     2341 gggtgcgcct gcggcggagc aggggacggg cggcgtggtg gggcatgcgt actcccgggc
     2401 gtggacgtcg gaccttcccc cgcatcatga ccggccgatc atgagcgaat cgtgagggcc
     2461 gcccctgctt ccgcgccgcg ggcgggcggg cgcgaggcgc agcacgaact ccgcgccgcc
     2521 gccggggcgg ggccggacgg cgagcatccc ccggtgcgcc tccgccacgc ggcgtaccag
     2581 cgagaggccg atccccgtcc ctccgcccgg cgaacggaac cgttccgtca gttccggcag
     2641 ccgggccgga tccaccccgg gccctcggtc ggccaccgcg acctcgggcc cccgcacggt
     2701 caccagcagc ccgtccccct ccggtgcctc cgccgcgcgg ccgtgccgca cggcgttgtc
     2761 cagcaggttc cgcacggcga cccgcaccag ggccgcgtcg gcctccacca cggactccgc
     2821 cagccgcagc cgagggcggg gcgcgctctc cggcagctcc gcgcagacct cctcgacgag
     2881 ctggtccagc cgcagcggct cagcccgcac cgcgtccacc gctgcctcca gccgcccccg
     2941 ggtcagcagg ttctccacga cgtcggacat ccgctcggcc gccctccgga tccgtggcag
     3001 atgggcgtcg agaccgcccg gagcgccggc cgccagctcc accgaaccgc gcatcaccgc
     3061 gaccggggtc cgcagctcgt gcgccgcgtc cgccagcagc ctctcctgct gctccaacgc
     3121 ctcccaggcc ggccgcatgc tgcgtcccga caggacgtgg ccggtcagcg ccgccagcgc
     3181 cgtcagcgca ccggccccga cgaccaggga gaggaccaga ctccggtgct cgtccgcgcc
     3241 gcgttccggg tcgccgacgg cgacggccgc gcccgccacc tcgtcggtgg cgtcgtggtg
     3301 gaagggcacg cccaggaggc gcaccggccg tcccgagcgg tcgcgcgcat cggcccgcgc
     3361 cgggccctcg tcggccatcg ccgcgcgggc caccgccgcc accggcccgg ccggcacggc
     3421 gaacggggcg gcccgccccc ggaagacggt gaccgggtcg gcgccgggcc gcccctccag
     3481 caccaggatc tgcggagtgc ccgcggtcgc ctcgtcgtcg tggaggccgt ccagccggat
     3541 ccggccgtcc tcgtagtaga cgagcgaggc ggcgacggag gcccggcggc gcatctcgtc
     3601 gtactcggcg tcctggcgcg cgcggtcgtc ggtacggatc accagccagg acaggcaggc
     3661 gagcccgacg acggtgatca gggtgtaggc ggcggtcagc cgcagctgga gccggcgcag
     3721 ccgccgcctg gccggggagc ggcgcgtgac gggccctgcc atcaggaggg ttccgtgcgc
     3781 gccgacagca ggaagccctg cccgcgcacc gtgtggacca gagcgggcga cccgagtttg
     3841 cgccgcaggc ccgcgatgac ggcgtccacc acattggaga ccgggtccgc catctcgtcc
     3901 cagcagtgct ccagcaggac ggtacgggtg acgacggcgt cccggtggac gaggagctgc
     3961 tggaggacgg cgtactcctt cgggctcagc gacagcagca ccccaccgcg ccgcacctcg
     4021 tggcgagcca cgtccatgac cacgtccgcg cagcgcagga acgacgggag ccggacggga
     4081 gcccggcggc tcaggccgcg tacgcgcagt accagctcgc gcatcgcgaa cggcttggcc
     4141 aggtagtcgt ccgccccgct ctccagcccc cgcacccgct cgtccaggga gtccagagcg
     4201 gtgaggcaga gcaccggcac cgaccacccg gcccgccggc ccgcctccaa cggggcgagg
     4261 gtgtcgccgg agggcaccat ccggtccagc accacgcagt cgtaggcgtt gacgtgcagc
     4321 agcacgtccg cctccggcca gtccgccgcg cagtccacgg cgaagccgcc gtcccgcagc
     4381 ccggccgcgg tcatctcccg cagatcctcg tcgtcctcca ccagcagcac tcgcacgggg
     4441 aggacgcctc cgattcgtgg tccgggacaa accccgcgcc ggggcgggcg ggggagaccg
     4501 gccatcctag gcggtccgtc cggcgcgaac ggacccggcc ggggaccgat ggccaccgct
     4561 cacgtgatgg cccgtacgcg ggagaaagcc gacccggcgt cggttttcgg acggcacgcc
     4621 ggacccggcg ggcccgccgg gtccggtccc ctttacccgg accagggcac cgcgtgtcgc
     4681 ggggtattcc tcggaaaggg aaaagcgaag ggtgtttctt ttctccgtgc tcgctttttt
     4741 gaaatattcc cggtgaatgc cgggcgccgc cattcgcttt tcttgctcga cggctgtggt
     4801 cgcgtttctt tgccggccgt cgggcccccg ctccagcccg gtcatctcgt ctccgtcgca
     4861 gggcaggggc ctggaagggg gtctccggac ccgggggccg agcctgtcga ggatagaaaa
     4921 aaaaccgcgt cgtctgtatc ttatcaacgc acgtgcatcg caggaacggc ttgccattca
     4981 catgacccct ggggggagtt gtgcccgaag cagcagtttt gatcgatccg gtgcgggcga
     5041 tggataccga ggccgacgtg acgcatcccg tggggatcga catggtgcat cgaaccaggt
     5101 cgcaggatgc ctttccgcgt ggctggatgc gcctggagca cgaccgtttc tcggtgaccg
     5161 cggtccttcc gcacgatcac gcgttcttcg cccccctggc cggcgaccgc cacgacccgc
     5221 tcctggtcgc ggaagcgatg cggcaggcgg ccatgctcgc cttccacgcc gggtacggcg
     5281 tgccgatggg acatcacttc ctgctgtcgg gcctggactt cgtgtgccac ctggagcatc
     5341 tgggcgtcgg tggcggcccc gccgaaatcg gtgtcgaggt gtactgctcc gacctcaggt
     5401 ggcgtgccgg gctgccggtg gcggggcgcg tgacctgggc ggtgcaccgc gacggcgggc
     5461 tggcggcgac gggcgtgggg gtgacacgct tcatcagccc cgaggtctac cggcggatgc
     5521 gcggagacgc ccccgccgaa ggcatagcga taccgcgcgc ggccccggtc cgcgccgacc
     5581 tggcgggccg agccaggacc gaggacgtgg tgctgtcgga gacggaccgg gacgggtggt
     5641 gggagctgag ggtggacacc cggcacccga cactgttcca gcgccccaac gaccacgtcc
     5701 cgggcatgct cctcctggag gcggcgcggc aggccgcctg cctggcggcg gggcccggag
     5761 gaatcgttcc cgcggaggtg gccagccggt tccaccgcta cgccgagttc gacagcccgt
     5821 gcctgatcgg cgcggtggtc cggccggggc ctgccgaggg tacggtgacg gcccgggtgt
     5881 ccgggcgcca gaacggcgaa ctcgtcttca ccgcggttct caccggcccg cgggtccacg
     5941 gctgagaggg cggcacgcag gtgattctcg tgacaggcgc gaccggggcg gtgggccgag
     6001 aggtcgccgc gcagctcgct gcggcgggcc ccgtgcggat tctcgcgcgc cgtcccgagc
     6061 gcctcggggc gttcgggaac agggccgagg tcgtggcggg ggcgtacgcg gaccggcagg
     6121 ccctcggccg ggcgctgcgg ggtgtcacct cggtcttcct ggtcaccaac agccccaccg
     6181 agcccgacga cgagcgggtc gccgcggcgg cggccgaggc gggcgtgcgc cacctggtca
     6241 agctgtccat gatggcggtg gaggagccgg acgcgtccga cctcatcacc cggctccagc
     6301 gggagaacga gcggacgatc cgtgaatcgg gggtcccctg gacgttcgtc cgcccccgaa
     6361 cgttcatgtc caacacgctg tcctgggcgg ccggcatacg ctccgatggg gtggtgcgcg
     6421 cgctccacgg cgacgcgccg gtcgcctgcg tggacccgcg cgatgtcgcc gcggtcgccg
     6481 tggccgccct caccggcacg ggccacgagg gcagggcgta cgccgtgtcg gggccggagg
     6541 cgatcacggc gcgggagcag actgcccagc tctcccgggc gctggggcgg cctctgcgct
     6601 tcgaggagtt gggcgtggcc gccgcgcgta ccgcgctgct cgcgaagtac ccgcgtcccg
     6661 tcgccgaggc gttcctggag agcgcggagc ggcagcgggc aggggccaag gcgggggtcg
     6721 tctccacggt gcggcagctg acgggtcgct cggcccggcc ctacaacgtc tggtcggccg
     6781 accacgcgga cgcgttcacc gggcgagacg accgatagcc gggggagtgg gacgcgggcg
     6841 cccaatgacg catcatccgg acgcccgctg acgtggcatc acagaccgag cggaaaagtc
     6901 tgatgatgcg catttacctt ttccgcttcg tctgagctcc gctcacgttc cgcttctggc
     6961 gagcggggcg ggtgtggccg gaaagtgtcc gggtgtgcac gtcgaattct cctgttccct
     7021 ggaaaaccga ccggtccgtt ggttggatga ctccatgtcg ccctcccacc gctccggcgt
     7081 ggagccctcc gccgggcccg gccccgcccc tctccgggga cggttccggt gaccaaacag
     7141 gagcgcgccg cccgcacccg ccaggcgctg atccgctccg ccgccgccgt cttcgagcag
     7201 cacggttacg cgcaggcccg gttggcgcag atcagctccg gcgcgggcgt cagcacgggc
     7261 gcgttgcact tccacttcga caacaaggcc gccgtggcgc aggcggtggt ggcggaggcg
     7321 tcccggaggc tgcgcgagtt gtcggccgcg gtccgccgag ggacggacaa cgcgctgcaa
     7381 gccctggtcg acacctcgca cgccctgctg gaacggctgc gggacgaccc ggtgagccgg
     7441 gccgggttcc ggctgagctg cgacggcgac gaggccgccg tccccgacct gaggctggag
     7501 tggcacgagc gggtacggga gctgctggac gaggccgccg ccacgggcgc gctcgccgag
     7561 ggcgtctccc gtgaggacac ggccgccgcc accgtcgcgg tgacggcagg gttcgaggcg
     7621 ctcggccgga acgactccgc gtggctggcc ccgcccatgc tgaccggatt ctggaactta
     7681 ctgctgcccc ggatcgccgc cgccgagacc gtggcccacg tggacccgtc gggcgcccgc
     7741 gcggcggaac cggcgctccg cgtgcccggc cccgccctcg cgaggcccgc caggggcgcg
     7801 gtggagccgg ccgcgggggt gaactgaccg gcggctccga cagggagccc gcccctcagc
     7861 gcccgcccgc cgggccccgg cccgcatcgt cgaacagatc cctccggccc gcgtcggcac
     7921 cccagctcgc cagcagacgc agaccctcgg ccgacgccga gccgggctcc gcgtggtacg
     7981 tcaccagatg ctgctcctcg tcgtcgggga ggttcagcgt ctcgtacgac agcgtcaggt
     8041 ccccggccag gggatgccgg acgagcttcg tgccgtggcc cttctccttg acgttgtgcg
     8101 tcgcccacag ccgtcggaac tcctcgctct tgaccgagag ctcgcccaca agcgccgaca
     8161 gctccgggtc gtcggggtgg cagcccgcgt acagccgcag gtagctgacc atgtccgacg
     8221 ccttggagtc ccagttcacg aacaggtccc ggtaggcggg cgagaggaac accagacgcg
     8281 cccagttgcg ttcgccgggc ggcagcgctc cccagtcgcc gaacaccgcg gccgccatcg
     8341 gattccaggc gaggatgtcg gagcgcgccc cggtcacgta cgcgggaacg ccctccatgc
     8401 tctccaagag gtacagcagc ccggtccgca cccgctgcgc cctcgccggg cggcgcttct
     8461 tcttgtgccg cgccggcctc gccagatgtg tcagatgcgc gtgctcggcg tcggtgagcc
     8521 gcagcgcgcg ggcgatcgcg tccagcacct ccgccgacac gttgcgtccg ttgccctgct
     8581 ccagacgcgt gtagtacgcc acggacaccc cggccagctg cgccagctcc tcccggcgca
     8641 gacccggtac ccggcgatgc cgcccgaact ccggcagtcc cacgtcctgc ggctggagct
     8701 tggccctccg ggtgcgcagg aactcgctga gctcggcgcg ccggtccagc ggctcggggg
     8761 aggcggcctc cggctgatcg tccataccct ccattattcc gccgattccc ggccgcgggc
     8821 cggtgagcct gtccccgtca gtggtaggac cgccggacgt aggcagagcg gtggcctggg
     8881 tgaacggcgc tcagggtgtg acgctcgaag acgtacccac cgcaccaccc agaggagaac
     8941 ctccggcatg accaccgttg ccgcctacgc cgctccccgt gccaaggccc cgctggagcg
     9001 caccacgatc gagcgccgcc cggtgggcga gttcgacgtc ctgatcgaca tcaagttcgc
     9061 cggtatctgc cattccgaca ttcaccaggc ccgcgacggc tggggcgagg gcatcttccc
     9121 gatggtgccc ggacacgaga tcgccgggat cgtcgccgag accgggcccg gggtgaccgc
     9181 gttcaaggtc ggcgaccgcg tcggcgtcgg atgcatggtc gactcctgcg gacagtgcga
     9241 cgcctgccgc gcgggccggg agcagcactg cgccgagggg aacacccaga cgtacaacgc
     9301 gctcgaccgc agcggcgagc ccacgtacgg cggctactcg acgcacatcg tcgtgcgcga
     9361 cgcgttcgtc gtctcgatcc ccgagggcat cgccctcgac gaggccgcgc ccctgctgtg
     9421 cgcgggcatc accacgtact ccccgctcaa gcgctggggc gtcggccccg gcaagaaggt
     9481 ggctgtcgtc ggtctgggcg gcctcggcca catggccgtc aagatcgcgc acacgctcgg
     9541 cgccgaggtc accgtcctct cccagacgct gcggaagaag gacgacgggc tcaagctcgg
     9601 cgccgaccac tactacgcga ccggcgaccc ggcgaccttc gaggaactgg ccgggacctt
     9661 cgacgtcatc ctctccacgg tctcggcccc gctggacttc ggcgcctacc tcggcctgct
     9721 gcggaccgac gggaccctgg tgaacgtcgg cgccccggag gagccgatct cgctcaacct
     9781 cttctcgctc atcggcggtg acaaggcgat cgccggctcc atgatcggcg ggatcgggga
     9841 gacccaggag atgctggact tctgcgccgc gcacgggctc ggcgcggaga tcgaggtgat
     9901 cgaggcgagc cgggtgaacg aggcgtacga acgcgtgctc gcgagcgatg tccgctaccg
     9961 cttcgtgatc gacgccgcga ccatctgagg cggtggccgc ggagtgccac acccgtacgc
    10021 cccgagccgt cgaccggctc ggggcgtacg ggttgagggc tcgggcgcgg tccggctcgt
    10081 agaggggcgc gtcgtcaggc gggatccggc ggggcggccg agcggcgcgc cccttccagc
    10141 acggccgcca gcagccccgg gaagagcgcc tccagatcgt cctcgcgcag ccggctcatc
    10201 cgggaggtcc cgtcgtagtg ctggcggatg acgcccgcct cgcgcagcac cttgaagtgg
    10261 tgcgtgctgg tggacttgct caccggcagg tcgaacgcga tacaggccat gtcctcgtgt
    10321 ccgttcgcca actcccgcgc gatgcgcagc cgtaccgggt cggccagcgc gtgcaggacc
    10381 gcctccagac ggatctcctc cgggcgcgga tgggcgatcc gacgtccgga cgctgcggcg
    10441 cgctcggggg tggacagctc aggggtggac atggggcctc ctcgggtccg gcctccaaag
    10501 tacgaatatc gacgtagttt gacaaccgcc gtactacgat ggttatcgtt gtttcctcgg
    10561 cgccgctcgc ctgcggcgac cccccgaccc gaggagttcc accggtgagt gccctgttca
    10621 cccccctgac cctgcggtcc ctggagatcc ccaaccgcgt gtggatgtct ccgatgtgca
    10681 tgtactccgc tgcccacgag gggccggagg caggcgttcc gaccgacttc cacctcaccc
    10741 atctcgcggg ccgcgccgcc ggcggcgcgg ggctcgtgat ggcggaggcg accggggtgc
    10801 gccccgacgg ccggatcagc ccgtgggacc tgggcctgtg gaacgaccgc cagcaggagg
    10861 cgttcacccg catcgcggcg gcgatcgcct cccacggatc ggttccggcg atccagatcg
    10921 cccatgccgg gcggaaggcg tccac
//
