LOCUS       NZ_CP070260            10399 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces californicus strain FDAARGOS_1210 chromosome.
ACCESSION   NZ_CP070260
VERSION     NZ_CP070260.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces californicus
  ORGANISM  Streptomyces californicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 624.55x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016906265.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:02:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,147
            Genes (coding)                    :: 7,032
            CDSs (with protein)               :: 7,032
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 36 of 115
            Pseudo Genes (incomplete)         :: 97 of 115
            Pseudo Genes (internal stop)      :: 12 of 115
            Pseudo Genes (multiple problems)  :: 27 of 115
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:25:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6520457
            Orig. end                         :: 6530856
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            47..1105
                     /locus_tag="I6J40_RS28190"
                     /old_locus_tag="I6J40_28180"
     CDS             47..1105
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 1.8e-23.
                     Score: 75.0. Matches aSDomain:
                     nrpspksdomains_I6J40_RS28190_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070339.1"
                     /locus_tag="I6J40_RS28190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28180"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_030117377.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.6e-17, bitscore:
                     59.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLAPIPAAAAEFAPETTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRIGAGSFDAVEAARTGFARLVRVHPDRVATGASVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRMRYAPLDGLAAAVRPTTALVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDATQAAGWLPVDAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQETLPRLFA
                     GWVSAGPPWAGNYGPLEHLAPTARGFDEPPAFLSYHGAEHSLALLAEVGADTLHAHATG
                     LAARLRAGLTGLGRAVVPGESAIVSVPGLAGREPALAEAGIAVSAPVGNLRISCHLYNT
                     EADIDRVLEFLA"
     aSDomain        104..1087
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS28190_Aminotran_5.1"
                     /evalue="1.80E-23"
                     /label="I6J40_RS28190_Aminotran_5.1"
                     /locus_tag="I6J40_RS28190"
                     /protein_end="347"
                     /protein_start="19"
                     /score="75.0"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRIGAGSFDAVEAARTG
                     FARLVRVHPDRVATGASVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRMRY
                     APLDGLAAAVRPTTALVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDATQAAGWLPV
                     DAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQETLPRLFAGWVSAGPPWAGNYGPLEHL
                     APTARGFDEPPAFLSYHGAEHSLALLAEVGADTLHAHATGLAARLRAGLTGLGRAVVPG
                     ESAIVSVPGLAGREPALAEAGIAVSAPVGNLRISCHLYNTEADIDRV"
     gene            complement(1133..1708)
                     /locus_tag="I6J40_RS28195"
                     /old_locus_tag="I6J40_28185"
     CDS             complement(1133..1708)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488166.1"
                     /locus_tag="I6J40_RS28195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28185"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_030196338.1"
                     /transl_table=11
                     /translation="MTTGRATPAEAACTDAPDAQRAPAPDTACADAPNACAVAPPAAAL
                     GGPVSHAVSRVARLHRIAAGKVLKGVGLYPGQELLMTHLWDRGAVRQTELIRALELDPS
                     TVTKMLQRLEQTGHVRRRPDPADRRAVLVEATDDSCALHTAVETAWGRLEEQTLAGLET
                     AERDELVRLLAKVEENLCQETADCPDRA"
     gene            1873..2943
                     /locus_tag="I6J40_RS28200"
                     /old_locus_tag="I6J40_28190"
     CDS             1873..2943
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="I6J40_RS28200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28190"
                     /product="alkene reductase"
                     /protein_id="WP_030117379.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTPLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLVITEGIQPSVVGQGYPSTPGLHSAEQVASWRAVTDAVHAAGGRIFAQIMHTGR
                     IGHPVLLPDGLVPVGASPVTAEGQVYTHAGPKDFVEPRELTDAEVRATVDDFVTAARNA
                     VEAGFDGVELHGANGYLIHQFLAPNTNLRTDAWGGSDEARTRFAVEVVKAVAAAIGPER
                     TGLRISPGNPYNGIDEPAPEATYTALVREIEPIGIAYLHILEAVPIRELTLALRKAFSG
                     AVVLNAHTEGPTGPGDHTVIDDGIADLISYGALFLANPDLPARLKAGGPFNTPDPATFF
                     GGDAKGYTDYPALDAV"
     gene            complement(2919..3953)
                     /locus_tag="I6J40_RS28205"
                     /old_locus_tag="I6J40_28195"
     CDS             complement(2919..3953)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="I6J40_RS28205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28195"
                     /product="VOC family protein"
                     /protein_id="WP_030196341.1"
                     /transl_table=11
                     /translation="MTSRVRHITIDCADAYALAGFWAEVLGAPLSDDDLPGDPEALVEA
                     PGAAVLFVQVPDGKRTKNRVHLDVQPQDRSRDEEVERLLALGATLVADHRKPNGRGWAT
                     LADPEGNEFCVECSAAERAALTGTRLPVTADDVTSAVRLAVDVLAGAPADRWEEPAGSL
                     TWTCWETAEHLSDDLFAYAVQLGPRTPSLEREVPYRWAPERQGGPSNAVFADREAGPAG
                     LLATLEASGALLASMARTTPPGVRSYHGYGVSDPEGFAAMGVVEALVHTHDLARGLGLE
                     WSPPRDLCGRALARLFPDAPEGGDRWAVLLWATGRAELPGHPRRTAWRWDGRPLADQTA
                     SSAG"
     gene            complement(4101..4994)
                     /gene="thpD"
                     /locus_tag="I6J40_RS28210"
                     /old_locus_tag="I6J40_28200"
     CDS             complement(4101..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="I6J40_RS28210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28200"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_030117381.1"
                     /sec_met_domain="PhyH (E-value: 5.5e-38, bitscore: 130.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPISAKDLQGY
                     EHDGFLTVDQLITPDEVSVYRAELDRLVSDPAVRADERSIVEKQTQNVRSVFEVHKLSE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENEAQEPFAAP
                     IRRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="I6J40_RS28215"
                     /old_locus_tag="I6J40_28205"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="I6J40_RS28215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28205"
                     /product="ectoine synthase"
                     /protein_id="WP_007450483.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.7e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5489..6751)
                     /gene="ectB"
                     /locus_tag="I6J40_RS28220"
                     /old_locus_tag="I6J40_28210"
     CDS             complement(5489..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 7.1e-72.
                     Score: 234.2. Matches aSDomain:
                     nrpspksdomains_I6J40_RS28220_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="I6J40_RS28220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28210"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_030120055.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.6e-76, bitscore:
                     254.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGITPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALQAYWADGQMEKQTLARGEQIEQTLL
                     AICAEEPTAHFRGRGLVWGMEFEDPARASAVCARAFRLGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARSVRETA"
     aSDomain        complement(5663..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS28220_Aminotran_3.1"
                     /evalue="7.10E-72"
                     /label="I6J40_RS28220_Aminotran_3.1"
                     /locus_tag="I6J40_RS28220"
                     /protein_end="363"
                     /protein_start="28"
                     /score="234.2"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALQAYWADGQMEKQTLARGEQIEQTLLAICAEEPTAHFRGRGLVWGMEFE"
     gene            complement(6968..7498)
                     /gene="ectA"
                     /locus_tag="I6J40_RS28225"
                     /old_locus_tag="I6J40_28215"
     CDS             complement(6968..7498)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="I6J40_RS28225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28215"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_078580606.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSKVLDLNSSY
                     SYLLWCRDFAATSAVARGENGEPIAFVTGYLRPDRPQTLVVWQVAVDQAHRGKGLAAAL
                     LDALTSRVAADQGLSSVETTITPDNTASERLFTSYAQRHDVGLEREVLFDGDLFPEETH
                     LPEVLYRIGPFTT"
     misc_feature    7509..7523
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7523..7535)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7654..7669)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7664..7679
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7664..7681)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7739..7755
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.98"
                     /tool="antismash"
     misc_feature    complement(7770..7788)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.05"
                     /tool="antismash"
     misc_feature    complement(7775..7788)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.75"
                     /tool="antismash"
     misc_feature    complement(7791..7805)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    7797..7810
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.67"
                     /tool="antismash"
     gene            complement(7808..8914)
                     /locus_tag="I6J40_RS28230"
                     /old_locus_tag="I6J40_28220"
     CDS             complement(7808..8914)
                     /NRPS_PKS="Domain: Aminotran_5 (17-286). E-value: 1.1e-29.
                     Score: 95.5. Matches aSDomain:
                     nrpspksdomains_I6J40_RS28230_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123620.1"
                     /locus_tag="I6J40_RS28230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28220"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_030196344.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.9e-23, bitscore:
                     79.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTAARFAAIERRVAALLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGATVVDLAVPFHTAVTADQVAEALAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERMAANPAAPRRSYLSLLDWKERWIDGGRTALPHAP
                     AQLEMLGLEACVARIEEEGAETVMARHASAAAATRAGALALGGGLEPYVREARDAAPVA
                     TTLRVPAGTDATALVAHAVAADPVLPLVAGGGALAKEMIRVNHYGPDATRGTVLSSLAA
                     VGAALADAGVRVDIEAARRAVSETWTSR"
     aSDomain        complement(8057..8863)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS28230_Aminotran_5.1"
                     /evalue="1.10E-29"
                     /label="I6J40_RS28230_Aminotran_5.1"
                     /locus_tag="I6J40_RS28230"
                     /protein_end="286"
                     /protein_start="17"
                     /score="95.5"
                     /tool="antismash"
                     /translation="AIERRVAALLATEQDVVITQGEALLPLEGCIRGGARPGSTALNVV
                     TGPYGQTFGNWLRDCGATVVDLAVPFHTAVTADQVAEALAAHPEIDFVSLVHAEAATGN
                     TNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVS
                     VSERAWERMAANPAAPRRSYLSLLDWKERWIDGGRTALPHAPAQLEMLGLEACVARIEE
                     EGAETVMARHASAAAATRAGALALGGGLEPYVREARDAAPVATTLRV"
     gene            9019..10119
                     /locus_tag="I6J40_RS28235"
                     /old_locus_tag="I6J40_28225"
     CDS             9019..10119
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="I6J40_RS28235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_28225"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_030117386.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.6e-18, bitscore:
                     65.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVDGRITYERPPGADRA
                     ETVRGWALPGLVDAHCHVGLDRHGPVDAATAERQALTDREAGTLLVRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREAKRGDGWVKLVGDWIDRS
                     VGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLRDLVEAGIDCVEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRRLYERRYDTVRAAYDAGIPV
                     YVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPALEEGAPADLVV
                     YDEDPRADVRVLAAPRHVVLEGRVVG"
ORIGIN
        1 actccgcgtg attgacggtg ccgcacgggg gacacaggct ggtcgtatga cgagcctcgc
       61 gcccatcccc gcggcagccg ccgagttcgc gccggagacc acctacctca acacctccag
      121 ctgggggctg ctgccccgcc ggacgatcgc cgccgtcaag gccctggccg acgagaacgc
      181 ggcgggccgc cggatcggcg cgggcagctt cgacgcggtg gaggcggcgc ggaccggctt
      241 cgcccggctc gtcagggtcc atccggaccg ggtcgccacc ggcgcctccg tcaccgtgca
      301 cgtcggcctg atcgccgctt cactgccgcc cggcgccgaa gtcctgtgcc ccgagggcga
      361 gttctcctcc gtggtcagcc cgttcgcgct ccgcggcgat ctgcggatgc ggtacgcccc
      421 gctcgacggc ctcgccgccg ccgtacgtcc cacgaccgcg ctggtcgcct tctccgccgt
      481 gcagtcggcg gacggccggg tggccgacct cgccgccgtc cgcgcggccg ccgccgccca
      541 cggggcccgg acgctgctgg acgccaccca ggcggcgggc tggctcccgg tggacgccgg
      601 cgcgtacgac tacaccgtca ccggcggctt caagttcctg ctctgcccgc gcggcacgtc
      661 gttcctcacc gtcacggagg aggcgcagga gaccctgccg cgcctcttcg cgggctgggt
      721 ctccgccggt ccgccctggg cgggcaacta cggtccgctg gagcacctcg ccccgaccgc
      781 ccgggggttc gacgagccgc ccgccttcct ctcataccac ggggccgagc actccctcgc
      841 cctgctcgcc gaggtcggcg ccgacacgct gcacgcccac gccacgggcc tcgccgcccg
      901 gctgcgcgcc gggctcaccg ggctggggcg cgcggtggtc ccgggggagt cggccatcgt
      961 ctccgtaccc gggctggcag gccgtgagcc cgccctggcg gaggcgggga tcgcggtctc
     1021 ggccccggtc ggcaacctcc ggatctcctg ccacctctac aacacggagg ccgatatcga
     1081 ccgggtgctg gagttcctgg cctgatccgt acggaaggcc ccggggcgcg cgtcaggcgc
     1141 ggtcggggca gtccgccgtc tcctggcaga ggttctcctc gaccttcgcc agcagccgga
     1201 ccagctcgtc ccgctccgcc gtctccagac ccgcgagcgt ctgctcctcc agccggcccc
     1261 aggccgtctc gacggccgtg tgcagcgcgc agctgtcgtc ggtcgcctcg accaggaccg
     1321 cccgccggtc ggccgggtcg gggcggcggc gcacgtgccc ggtctgctcc agtcgctgga
     1381 gcatcttcgt gacggtggac ggatcgagtt ccagcgccct gatcagctcc gtctggcgga
     1441 ccgccccccg gtcccacagg tgcgtcatca gcagctcctg ccccgggtag aggcccaccc
     1501 ccttgagcac cttgcccgcc gcgatccggt gcagccgggc gacccgggag acggcgtggc
     1561 tgacgggccc gccgagcgcc gccgccggag gcgccacggc gcaggcgttc ggcgcgtcgg
     1621 cgcacgccgt gtccggcgcg ggggcgcgct gcgcgtccgg cgcgtcggta caggcggctt
     1681 ccgcgggggt ggcgcggccg gtggtcatgg cgggctcctg aggtcctcgg gcggagagcg
     1741 gcggctctcg ggcggcggct tccgatgatg cccgacggtt tcccacgact ttaccttggt
     1801 cggccaatga atgggctaca gtggctcccg gcggcgatcg ttggccgacc acatactttc
     1861 tcaggagccc tcatgaccac cgctttcgac ccgatcgacc tggccggcac cccgctcgcc
     1921 aaccgcatcg ccctggcccc gatgacccgt agccgggccg gcgagggcgg caccgccacc
     1981 gagctgaccg ccacgtacta cgcccagcgc gcctcggccg gactcgtgat caccgagggc
     2041 atccagccct ccgtcgtcgg ccagggctac ccctccacgc ccggcctgca cagcgccgag
     2101 caggtcgcct cctggcgcgc ggtcaccgac gccgtgcacg ccgcgggcgg ccggatcttc
     2161 gcccagatca tgcacacggg ccgcatcggc caccccgtcc tgctgccgga cggtctcgtc
     2221 cccgtcggcg cctccccggt cacggccgag gggcaggtct acacccacgc cggaccgaag
     2281 gacttcgtcg agccgcgcga gctgaccgac gccgaggtcc gcgcgaccgt cgacgacttc
     2341 gtcaccgccg cccgcaacgc cgtggaggcc ggcttcgacg gggtcgaact gcacggcgcc
     2401 aacggctacc tcatccacca gttcctggcg cccaacacca acctgcgcac cgacgcctgg
     2461 ggcggctccg acgaggcccg cacccgcttc gccgtcgagg tcgtcaaggc cgtcgccgcc
     2521 gcgatcggcc ccgagcgcac cggactgcgc atctcgccgg gcaacccgta caacggcatc
     2581 gacgagcccg ccccggaggc cacgtacacc gcgctggtcc gggagatcga gccgatcggc
     2641 atcgcctacc tgcacatcct ggaggccgtc ccgatccgcg agctgaccct cgcactgcgc
     2701 aaggcgttct ccggggccgt cgtcctcaac gcgcacaccg agggccccac gggccccggc
     2761 gaccacacgg tgatcgacga cggcatcgcc gacctgatct cctacggggc gctcttcctc
     2821 gccaacccgg acctgcccgc ccggctcaag gcgggcggcc cgttcaacac ccccgacccg
     2881 gccaccttct tcggcggcga cgccaagggc tacaccgact acccggcgct cgacgccgtc
     2941 tgatccgcca ggggccgccc gtcccaccgc cacgcggtgc ggcgcgggtg gcccggcagc
     3001 tcggcgcggc cggtggccca cagcaggacg gcccaccggt cgccgccctc cggcgcgtcc
     3061 gggaagagcc gggccagcgc ccggccgcac agatcccggg gcggggacca ctccagaccc
     3121 agcccccgcg ccaggtcatg ggtgtgcacc agggcctcca ccacccccat cgcggcgaag
     3181 ccctccgggt cggagacccc gtacccgtgg tacgagcgca ctcccggcgg cgtcgtccgg
     3241 gccatcgagg ccagcagcgc cccgctcgcc tccagcgtgg cgagcagccc ggcgggcccg
     3301 gcctcccggt cggcgaagac cgcgttggac ggtccgccct gccgctcggg agcccaccgg
     3361 tagggcacct cccgctccag cgacggcgta cgggggccca actgcacggc gtacgcgaac
     3421 agatcgtcgc tcaggtgctc cgccgtctcc cagcaggtcc aggtcaggct gccggccggc
     3481 tcctcccagc ggtccgccgg ggccccggcc agcacgtcca ccgcgagccg cacggcggac
     3541 gtcacgtcgt cggcggtcac cggcagccgg gtcccggtca gcgccgcccg ctcggcggcg
     3601 gagcactcca cgcagaactc gttgccctcg gggtcggcca gcgtcgccca gccgcgcccg
     3661 ttcggcttgc ggtggtcggc caccagggtc gcgccgagcg cgagcagccg ctccacctcc
     3721 tcgtcccggg agcggtcctg cggctggacg tcgagatgga cgcggttctt ggtccgtttc
     3781 ccgtcgggca cctggacgaa gagcacggcc gcgccggggg cctcgaccag cgcctccggg
     3841 tcaccgggca ggtcgtcgtc ggagagcggc gcgccgagca cctcggccca gaagccggcg
     3901 agcgcgtagg cgtcggcgca gtcgatcgtg atgtggcgta cgcgagaagt catggcgcac
     3961 accctccccg ccgactcccg tcccggtcca ccgagttacg cacggggggc cgccgggccg
     4021 tacacgcgcc cccgcggcgg cgtacgcgcg aaggcccggg acggcgtacc gtcccgggcc
     4081 ccactcgcct gtgcgtacgg ctacctcacc ggggtgaagt cgcgcgcccc gatgaactcg
     4141 gggcggcgga tcggagccgc gaagggctcc tgcgcctcgt tctccacgct gttgaagacg
     4201 atgaagacgt tgctgcgcgc gtacggcgtg atgttgtcgc ccgagccgtg catggcgttg
     4261 cagtcgaacc acgtcgccga gccggccttg cccgtgaaga gcttgatgcc gtggcggtcg
     4321 gccatcttcg tcagcgcctc gtcggacggg gtgccggcgt cctgcatctg gagcgacttc
     4381 ttgtagttgt ccttcggcgt ctcgcccgcg cagccgagga acgacttgtg cgaaccgggc
     4441 atgatcatca gcccgccgtt ggtgtcgaag ttctcggtca gcgcgatcga cacggacacc
     4501 gtccgcatgt tcggcagacc gtcctccgcg tgccaggtct cgaagtccga gtgccagtag
     4561 aagcccgtgg ccccgaagcc cggcttcacg ttgatccgcg actggtggac gtagacgtcc
     4621 gagccgagga tctggcgggc gcggcccacc acccgctcgt cgcggaccag cccggcgaag
     4681 acctcgctga gcttgtggac ctcgaagacg gaccgtacgt tctgggtctg cttctcgacg
     4741 atcgagcgct cgtcggcgcg gaccgccggg tccgagacga gccggtcgag ctcggcccgg
     4801 tagacggaga cctcgtccgg ggtgatgagc tggtcgacgg tgaggaatcc gtcgtgctcg
     4861 tagccctgga ggtccttggc gctgatcgga cccggtgcgc ccggcgcgga ccagatgacc
     4921 gggtcctggc ggggagtggt catctcggcg gcgccgcgcg aggggtacag atcggcgcgt
     4981 acttcggtgg tcatggtggt tcaggcctcc tcggtcagca gggggtagac gccgttctcg
     5041 tcatgatcct cccgtccggt cacggggggg ttgaagacac acacgcagcg gaagtccgtc
     5101 ttgggccgca tggtgtgccg ctcgtgcccg tccagcaggt acatggtccc gggcgtgatc
     5161 cagtgcgtct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagcacg
     5221 gcctcgatgt ggttcgcgta ccacatggag gtctccgtac ccgcgtagag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcac tgaacgatcg gacgatcacg
     5401 gtgagctgtc gcctttctct tctctgcgcg ggccccggac ctcgcggtcc ggggcccctc
     5461 cttcttcgcc tgccccggca gcgccgggtc aggccgtctc gcggacggaa cgggccagcg
     5521 tgcgcaggcc ctcgtccagt tcctcggggg tgatggtcag cggcgggagc agcttgacga
     5581 cctcgctctg cgggccggag gtctccagca ggagccccag ccggaaggcg cgggcgcaga
     5641 ccgcggaggc gcgcgccggg tcctcgaact ccatgcccca caccaggccg cggccgcgga
     5701 agtgcgcggt cggctcctcg gcgcagatgg ccagtagcgt ctgctcgatc tgctcgcccc
     5761 gggccagggt ctgcttctcc atctggccgt ccgcccagta cgcctgcagc gcggcggcgg
     5821 ccgtgacgaa ggcggggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga
     5881 tgtccagctc cggcttgaac aggcagagcg acatcggcag gccgtagccg ctgatggact
     5941 tcgacagggt gacgatgtcc ggggtgatcc cggcctcctc gaaggagaag aagccgccgg
     6001 tgcggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcctgg cggtggcaca
     6061 ggtcctggag cgcgcgcagc cactcggcgc gggccacgtt gatgccgccc tcgccctgga
     6121 ccgtctccac gatcacggcc gcgggcttgt tcagcccgga gccctggtcc tccagcagcc
     6181 gctcgaacca gaggaagtcc gggacctggc cgtcgaagta gttgtcgaac ggcatcgggg
     6241 tgccgtgcac cagcgggatg ccggccccgg cgcgcttgaa cgcgttgccg gtgacggcga
     6301 gcgagcccag cgacatgccg tggaaggcgt tggtgaacga gacgacggac tcgcggccct
     6361 tgaccttgcg ggccagcttc agcgccgact cgacggcgtt ggtgccggtc gggccgggga
     6421 acatcacctt gtacggaagg tcgcgcgggc gcaggatcac gttctggaag gtctccagga
     6481 acgcgcgctt ggcggtggtc gccatgtcca ggccgtgggt gatgccgtcg cgctcgatgt
     6541 agtcgatcag cgctcgtttc agcaccgggt tgttgtggcc gtagttgagc gagccggccc
     6601 cggcgaagaa gtccaggtag gagtggccgt cctcgtcggt cagccgggct ccctgcgcgc
     6661 ggtcgaagac ggcgggccag ccgcggcagt agctccggac ctcggactcc agggtctcga
     6721 agacgctcag tgcgggcggg gtgatggtca cagcggatct cctgcgtgag ggggtgtgac
     6781 gggcggtggt ctggtgctcc ccgccgcacg gatgcggccg ggaccgcgcc gggtggtcgg
     6841 ccggcgcgaa aagtcgtggg accggggcgc acgggtcggg tgcgccggcc ggtgggcctg
     6901 cggatcggtg gatcggtgga tcggacggcg tacgggtcgg gtacgccggt gcccggaacg
     6961 ggacggctca ggtggtgaac gggcctatcc ggtagagcac ctcgggcagg tgcgtctcct
     7021 cggggaacag gtcgccgtcg aagaggacct cccgctccag tccgacgtcg tggcgctggg
     7081 cgtaggaggt gaacagccgc tcggaggcgg tgttgtccgg ggtgatcgtc gtctccaccg
     7141 acgacagtcc ctggtcggcg gcgacccggg aggtcagcgc gtccagcaga gcggcggcca
     7201 gtcccttgcc ccggtgggcc tggtcaacgg ccacctgcca tacgacgagc gtctgggggc
     7261 ggtcgggcct gaggtagccc gtcacgaagg cgatgggctc gccgttctcg ccccgggcga
     7321 cggcggacgt cgcggcgaag tcacggcacc acagcaggta gctgtacgag gagttcaggt
     7381 ccaggacctt ggagtcgcgg gcgatgcgcc agatcgcggc tccgtcctcc actcgcggtg
     7441 catcgatact gaggaattcg cttcgggcac gggcaaaatc tgctggtgcg gcggtcatgc
     7501 gaattgaatt tacccagcaa atttcgaaat tgcatcgtcg caaggggttg gtcggagcgc
     7561 ctgcctgtgt tatcaggcgt gcgtgagaac ccgcgcgatg cggaggtggt ttcatgcgct
     7621 ttgtcccgaa ttaatcgggc ataatgccgc tcgtgtggag tcggtcacag ggttgtaact
     7681 ctcgtggaac ctgtccgaat cacgaagttc gcggccagaa aaaactgacc cgtttaggtg
     7741 acgggaaagc gggcagaaga atgtgggaag ctgcttttct ataaagcagc ttcccacatt
     7801 atgaatatca gcggctcgtc caggtttccg aaacggcccg acgggcagcc tcgatatcga
     7861 cccgaacacc cgcgtccgcc agcgccgcgc ccacggccgc gagcgaggag agcaccgtgc
     7921 cccgcgtcgc gtccgggccg tagtggttga cccggatcat ctccttcgcc agcgccccgc
     7981 cgcccgcgac gagcggcagc acggggtccg ccgccaccgc gtgcgcgacc agcgcggtcg
     8041 cgtccgttcc ggccgggaca cgcagcgtcg tggccaccgg cgcggcgtcc cgcgcctcgc
     8101 ggacgtacgg ctccaggccg ccgcccagcg ccagcgcccc ggcccgggtc gccgcggccg
     8161 ccgaggcgtg gcgggccatc acggtctccg cgccctcctc ctcgatccgc gccacacacg
     8221 cctccaggcc cagcatctcc agctgcgccg gcgcgtgcgg aagcgcggtc cgcccgccgt
     8281 cgatccagcg ctccttccag tccaggaggg agaggtagga acggcgcggc gcggccgggt
     8341 tggcggccat ccgctcccag gcccgctcgc tcaccgagac cgcggagacc ccggcgggcc
     8401 cgcccatggc cttctgcgcg ccgatcacgc acaggtcgac gccccaggcg tccggcagca
     8461 gcggctcggc gcccaccgag gcgaccgcgt ccagcatgaa cagcgccccg tgcgcccgga
     8521 ccacctcgcc gatctccgcg accgggttgg tgttgccggt ggccgcctcc gcgtggacca
     8581 gcgagacgaa gtcgatctcc gggtgcgcgg cgagcgcctc ggcgacctgg tcggcggtca
     8641 cggcggtgtg gaagggcacg gcgaggtcga cgacggtcgc gccgcagtcc cgcagccagt
     8701 tgccgaaggt ctgcccgtac ggtccggtga ccacgttcag cgcggtcgac ccggggcgcg
     8761 cgccgccgcg gatgcagccc tccaggggca gcagggcctc gccctgggtg atgacgacgt
     8821 cctgctcggt ggccaggagc gcggccaccc gccgttcgat ggccgcgaaa cgcgcggcgg
     8881 tcagcggggg gaggtcgagg aaggggtgcg tcacggcggg gctctcttcg gtgtcgttcg
     8941 tcgggggcgg gcctgggcgg tccgccgaca tgttcccggg ccctggcagc ctaccgacgg
     9001 cctcgtacag tgcggcatat gagtgatcag gaggagcagc gggtgctgcg ggtgaagggt
     9061 cgggtcctgg tcggcccgga cgaggtgcgg gacgaactgt gggccgtgga cggccggatc
     9121 acgtacgagc ggccaccggg cgcggaccgg gccgagacgg tacggggctg ggcgctgccc
     9181 ggcctggtcg acgcgcactg ccacgttggc ctcgaccggc acgggcccgt cgacgccgcg
     9241 accgccgaac gccaggcgct gaccgaccgg gaggccggca cgctgctcgt ccgggacgcc
     9301 ggatcgccct ccgacacccg gtggatcgac gaccgcgagg acctgccgaa gatcatccgg
     9361 gccggccgcc acatcgccag gacccgccgc tacatccgca actacgccca cgagatcgag
     9421 cccggcgacc tcgtcgcgta cgtggcgcgg gaggcgaagc gcggcgacgg ctgggtcaag
     9481 ctggtggggg actggatcga ccgctccgtc ggggacctga ccgcgtgctg gccgcgcggc
     9541 gaggtggagg cggccatcgc cgaggcgcac cggctggggg cccgggtcac cgcgcactgc
     9601 ttcgccgagg agtcgctgcg cgatctggtg gaggcgggca tcgactgcgt cgagcacgcc
     9661 acgggcctca ccgaggacac catcccgctc ttcgccgaac gcggggtggc gatcgtcccg
     9721 acgctggtca acatcgccac cttccccgac ctggcggcgg gcggcgaggc gaagttcccc
     9781 cgctggtcgg cccatatgcg gcggctgtac gagcgccgct acgacaccgt gcgcgccgcg
     9841 tacgacgcgg gcatcccggt ctacgtcggc accgacgcgg gcggttccct ggcgcacggc
     9901 ctggtcgccg gcgaggtggc cgagctggtc aaggcgggca tcccgccgct ggaggcgctg
     9961 tccgccaccg cgtggggggc gaggcgctgg ctggggcggc cggcgctgga ggagggcgct
    10021 ccggcggacc tggtcgtgta cgacgaggac ccgcgcgcgg acgtgcgggt gctggcggcc
    10081 ccgcggcacg tcgtgctcga agggcgggtg gtcgggtgac ccggaacggc ggcgccgtcg
    10141 ggtgagcgga cgcggcgcgc tgcggggagc cgtgttgacg agcggtgacg gacgcgcctc
    10201 cggctcgttg ttcttcctcc gcgcgccgcc ccaggcgcgg tctccggacg gcttgtacga
    10261 ccgatgccgg gtgaatcgag tgacgacagg gaacgcccgt gccgaaagat tcgaatgtga
    10321 gcgctaaaac caccctttgg ggtgaactca cgccaggtgt ccgccagttc acccacagtg
    10381 cgtaaagatt cacggagtc
//
